CN105223316A - A kind of method of quick discriminating donkey-hide gelatin quality - Google Patents
A kind of method of quick discriminating donkey-hide gelatin quality Download PDFInfo
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- CN105223316A CN105223316A CN201510666413.8A CN201510666413A CN105223316A CN 105223316 A CN105223316 A CN 105223316A CN 201510666413 A CN201510666413 A CN 201510666413A CN 105223316 A CN105223316 A CN 105223316A
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Abstract
The method of a kind of quick discriminating donkey-hide gelatin quality of the present invention, chooses the qualified Donga donkey-hide gelatin of quality as standard reference sample; By the donkey-hide gelatin sample dissolution after pulverizing in distilled water, then be placed in sample bottle and seal; Be placed in by sample bottle in the sample disc of electric nasus system, the analytical parameters of setting electric nasus system, carries out analytical test by electric nasus system; Gathered the sense of smell finger print information of donkey-hide gelatin sample by electric nasus system, the analysis time of each sample is 120s; The sensor array of electric nasus system gathers the smell that donkey-hide gelatin sample distributes, and the data separate SPSS Software of Data Statistics of collection is carried out hierarchial-cluster analysis to it, realizes the quick discriminating to donkey-hide gelatin quality.Inventive samples pre-treatment is simple, without the need to any organic solvent, natural pollution-free, has cheapness, the feature such as quick, objective.
Description
Technical field
The invention belongs to detection analysis field, relate to a kind of donkey-hide gelatin, specifically a kind of method of quick discriminating donkey-hide gelatin quality.
Background technology
" donkey-hide gelatin, herbal classic is top grade.Great scape is said: ' going out Donga, therefore named donkey-hide gelatin ' ".Donkey-hide gelatin has nearly 3000 history in China, with ginseng, pilose antler being called " nourishing Triratna ", begins to be loaded in China's pharmacy the earliest classical---and the Shennong's Herbal of the Eastern Han Dynasty, is respectfully called enrich blood " panacea " by ancient Chinese medicine doctor.The traditional Chinese medical science thinks that donkey-hide gelatin taste is sweet, property is put down, and enriching yin of enriching blood, moisturizes the effects such as hemostasis, and in treatment is not sick, effect is remarkable.Current clinical medicine research shows, donkey-hide gelatin has to nourish blood enriches blood, beautifying face and moistering lotion, delays to calculate old, improves immunity, replenishes the calcium and the effect of inhibition tumor cell.
Well water and Donga underground water are same water sources, the top grade donkey-hide gelatin of the most applicable refining.Therefore Donga donkey-hide gelatin becomes the leading enterprise of domestic donkey-hide gelatin industry, and its quality is also superior.And donkey-hide gelatin quality in the market dragons and fishes jumbled together, very different, more have illegal retailer to palm off donkey-hide gelatin of an established trade mark and fake and sell, work the mischief to the health of consumer, cause puzzlement to the operation of dealer.Therefore, in the urgent need to realizing the discriminating of donkey-hide gelatin quality, and differentiate particularly important fast.
Electronic Nose be development in recent years get up a kind of analysis, identification and detection of complex smell and volatile ingredient instrument, be that simulated animal olfactory organ develops a kind of high-tech product.The Electronic Nose Technology response time is short, detection speed fast, unlike Other Instruments, as gas chromatograph, high performance liquid chromatograph need complicated preprocessing process; It is wide that it measures scope of assessment, and it can detect various different types of food, and can avoid personal error, reproducible.It in a lot of fields especially food service industry play more and more important effect.
Hierarchial-cluster analysis (HCA) carries out cluster so that the distance of distance is close and distant for standard.First be polymerized to class apart from close sample (or variable), be polymerized to class after distance is mutually far away, process goes on always, finally all samples (or variable) is polymerized to a class.In order to reflect above Hierarchical Clustering process intuitively, whole categorizing system can be drawn as a Cluster tendency (dendrogram).Clearly can be found out the accumulation process of whole sample by Cluster tendency, thus the classification to whole sample can be drawn.Hierarchial-cluster analysis (HCA) model result is directly perceived, concisely.
Summary of the invention
For above-mentioned technical matters of the prior art, the invention provides a kind of method of quick discriminating donkey-hide gelatin quality, the technical matterss such as method will solve the method testing cost costliness of HPLC of the prior art and sensory evaluation donkey-hide gelatin quality, detection time is long, experimental error is large of described this quick discriminating donkey-hide gelatin quality.
The invention provides a kind of method of quick discriminating donkey-hide gelatin quality, comprise the steps:
(1) the qualified Donga donkey-hide gelatin of quality is chosen as standard reference sample;
(2) by the donkey-hide gelatin sample dissolution after pulverizing in distilled water, then be placed in sample bottle and seal; (3) be placed in the sample disc of electric nasus system by sample bottle, the analytical parameters of setting electric nasus system, carries out analytical test by electric nasus system; The analytical parameters of described electric nasus system is: heat time 5 ~ 6min, heating-up temperature 40 ~ 50 DEG C; Well heater hunting speed 500 ~ 600rpm, vibration 3s, interval 1s; Sampling volume: 500 μ L; Sample introduction speed: 500 μ L/s; Data acquisition time: 120s; Lag time: 1080s;
(4) by the sense of smell finger print information of the donkey-hide gelatin sample of electric nasus system acquisition step (2), the analysis time of each sample is 120s;
(5) by the sensor array of electric nasus system to step 1) in the smell that distributes of donkey-hide gelatin sample gather, the data separate SPSS Software of Data Statistics of collection is carried out hierarchial-cluster analysis to it, described cluster analysis selects group distance as clustering method, Euclidean distance quadratic method as measuring distance method, and the formula of the method for described measuring distance is:
x
1krepresent the kth dimension coordinate of first point, x
2krepresent the kth dimension coordinate of second point, it is a point set that n ties up Euclidean space, its each point can represent (x (1), x (2), ... x (n)), wherein x (k) (k=1, 2...n) be real number, be called a kth coordinate of x, two point x and y=(y (1), y (2) ... y (n)) between distance d (x, y) formula is above defined as, Donga donkey-hide gelatin sample is as standard reference sample, then algorithm carries out Treatment Analysis to data accordingly, draw the tree-shaped analysis chart of corresponding HCA donkey-hide gelatin quartile length, by distance, cluster is carried out to donkey-hide gelatin sample.
Further, described electric nasus system is French AlphaMOSFOX4000, sensor model number is to organic compound fast-response probe TA/2, LY2/AA, P30/2, T30/1, gas-sensitive sensor T40/1, T40/2, P40/1, P40/2, the LY2/LG stronger to oxidability, to inflammable gas fast-response probe P10/1, P30/1, to toxic gas fast-response probe PA/2, LY2/gCT1, LY2/G, LY2/Gh, to inflammable gas fast-response probe P10/2, LY2/gCT, to aromatics fast-response probe T70/2.
Concrete, in step (2), after crushed, get 0.50g donkey-hide gelatin sample dissolution in the distilled water of 5.0mL80 DEG C, be placed in 10mL sample bottle and with silica gel pad sealing, leave standstill 10 ~ 15min.
The present invention selects group distance (Between-groupslinkage) to carry out cluster analysis (HCA) as clustering method, Euclidean distance quadratic method (SquaredEuclideanDistance) as measuring distance method, algorithm carries out Treatment Analysis to data accordingly afterwards, draws the tree-shaped analysis chart of corresponding HCA donkey-hide gelatin quartile length.The method not only simplify the operating process of traditional quartile length, saves the time, and analysis result is reliably objective, fully can reflect the quality characteristic of donkey-hide gelatin sample.
The present invention differentiates that the method for donkey-hide gelatin quality is the electric nasus system utilizing the sensor of 18 models to form, and with SPSS Software of Data Statistics for instrument, coupling system clustering methodology (HCA) realizes the quick discriminating to donkey-hide gelatin quality.Inventive samples pre-treatment is simple, without the need to any organic solvent, natural pollution-free, and does not recycle liquid chromatography, and gas chromatography mass spectrometry and sensory evaluation are as the method for donkey-hide gelatin Quality Identification.
The present invention compares with prior art, and its technical progress is significant.The method of qualification donkey-hide gelatin quality of the present invention, more cheap, more objective, faster, more fully more different donkey-hide gelatin, is easy to universal and promotes, having certain positive effect.Measurement result of the present invention objective reliable, solve in discrimination process accidentalia occurs and manpower is differentiated poor efficiency, the high cost differentiating to classify.
Accompanying drawing explanation
Fig. 1 is the hierarchial-cluster analysis figure of donkey-hide gelatin sample.
Embodiment
For better understanding the present invention, below in conjunction with accompanying drawing and instantiation mode, the present invention is described in further detail.
Embodiment 1
A method for quick discriminating donkey-hide gelatin quality, comprises following step:
Step 1): the donkey-hide gelatin sample (as shown in table 1) got after 0.50g pulverizing is dissolved in the distilled water of 5.0mL80 DEG C, is placed in 10mL sample bottle and with silica gel pad sealing, leaves standstill 10 ~ 15min, each donkey-hide gelatin sample revision test 3 times;
Step 2): the analytical parameters of setting electric nasus system:
The present invention's electric nasus system used is the FOX4000 Electronic Nose fingerprint analysis system of French AlphaM.O.S company.Analytical parameters is: heat time 5 ~ 6min, heating-up temperature 40 ~ 50 DEG C; Well heater hunting speed 500 ~ 600rpm, vibration 3s, interval 1s; Sampling volume: 500 μ L; Sample introduction speed: 500 μ L/s; Data acquisition time: 120s; Lag time: 1080s.
Step 3), by step 1) sample bottle be placed in sample disc, carry out analytical test by electric nasus system, the analysis time of each donkey-hide gelatin sample is 120s.
Donkey-hide gelatin sample is used in table 1 experiment
Step 4), sensor array is to step 1) in the smell that distributes of donkey-hide gelatin sample gather, the data separate SPSS Software of Data Statistics of collection is carried out hierarchial-cluster analysis to it, herein select group distance as clustering method, Euclidean distance quadratic method (
x
1krepresent the kth dimension coordinate of first point, x
2krepresent the kth dimension coordinate of second point, it is a point set that n ties up Euclidean space, its each point can represent (x (1), x (2), ... x (n)), wherein x (k) (k=1,2...n) be real number, be called a kth coordinate of x, two point x and y=(y (1), y (2) ... y (n)) between distance d (x, y) formula is above defined as .) carry out cluster analysis as measuring distance method, algorithm carries out Treatment Analysis to data accordingly afterwards, draws the tree-shaped analysis chart of corresponding HCA donkey-hide gelatin quartile length, as shown in Figure 1.In figure, by distance, cluster is carried out to donkey-hide gelatin sample, using No. 1 Donga donkey-hide gelatin as standard reference sample, give tacit consent to that it is best in quality.Immediate with it is No. 3 donkey-hide gelatin samples, is secondly 5,17, and No. 13 donkey-hide gelatin samples are 4,15,8,2 afterwards, 19,12, and No. 7 donkey-hide gelatin samples are No. 16 donkey-hide gelatin samples again, are finally 6,10,9,11,18, No. 14 donkey-hide gelatin samples.As can be seen from the above results, Electronic Nose coupling system cluster analysis (HCA) model successfully can differentiate the donkey-hide gelatin of different quality.
Foregoing be only the present invention conceive under basic explanation, and according to any equivalent transformation that technical scheme of the present invention is done, all should protection scope of the present invention be belonged to.
Claims (3)
1. differentiate a method for donkey-hide gelatin quality fast, it is characterized in that comprising the steps:
(1) the qualified Donga donkey-hide gelatin of quality is chosen as standard reference sample;
(2) by the donkey-hide gelatin sample dissolution after pulverizing in distilled water, then be placed in sample bottle and seal;
(3) be placed in the sample disc of electric nasus system by sample bottle, the analytical parameters of setting electric nasus system, carries out analytical test by electric nasus system; The analytical parameters of described electric nasus system is: heat time 5 ~ 6min, heating-up temperature 40 ~ 50 DEG C; Well heater hunting speed 500 ~ 600rpm, vibration 3s, interval 1s; Sampling volume: 500 μ L; Sample introduction speed: 500 μ L/s; Data acquisition time: 120s; Lag time: 1080s;
(4) by the sense of smell finger print information of the donkey-hide gelatin sample of electric nasus system acquisition step (2), the analysis time of each sample is 120s;
(5) by the sensor array of electric nasus system to step 1) in the smell that distributes of donkey-hide gelatin sample gather, the data separate SPSS Software of Data Statistics of collection is carried out hierarchial-cluster analysis to it, described cluster analysis selects group distance as clustering method, Euclidean distance quadratic method as measuring distance method, and the formula of the method for described measuring distance is:
k=1,2..n, x
1krepresent the kth dimension coordinate of first point, x
2krepresent the kth dimension coordinate of second point, it is a point set that n ties up Euclidean space, its each point can represent (x (1), x (2), ... x (n)), wherein x (k) (k=1, 2...n) be real number, be called a kth coordinate of x, two point x and y=(y (1), y (2) ... y (n)) between distance d (x, y) formula is above defined as, Donga donkey-hide gelatin sample is as standard reference sample, then algorithm carries out Treatment Analysis to data accordingly, draw the tree-shaped analysis chart of corresponding HCA donkey-hide gelatin quartile length, by distance, cluster is carried out to donkey-hide gelatin sample.
2. the method for a kind of quick discriminating donkey-hide gelatin quality as claimed in claim 1, it is characterized in that: described electric nasus system is French AlphaMOSFOX4000, sensor model number is to organic compound fast-response probe TA/2, LY2/AA, P30/2, T30/1, the gas-sensitive sensor T40/1 stronger to oxidability, T40/2, P40/1, P40/2, LY2/LG, to inflammable gas fast-response probe P10/1, P30/1, to toxic gas fast-response probe PA/2, LY2/gCT1, LY2/G, LY2/Gh, to inflammable gas fast-response probe P10/2, LY2/gCT, to aromatics fast-response probe T70/2.
3. the method for a kind of quick discriminating donkey-hide gelatin quality as claimed in claim 1, it is characterized in that: in step (2), after crushed, get 0.50g donkey-hide gelatin sample dissolution in the distilled water of 5.0mL80 DEG C, be placed in 10mL sample bottle and with silica gel pad sealing, leave standstill 10 ~ 15min.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106645606A (en) * | 2016-12-28 | 2017-05-10 | 东阿阿胶股份有限公司 | Evaluation method of sensory quality of ass-hide glue |
| CN107536850A (en) * | 2017-05-09 | 2018-01-05 | 东阿阿胶股份有限公司 | Excellent donkey-hide gelatin of a kind of odor qualities and preparation method thereof |
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| CN106645606A (en) * | 2016-12-28 | 2017-05-10 | 东阿阿胶股份有限公司 | Evaluation method of sensory quality of ass-hide glue |
| CN107536850A (en) * | 2017-05-09 | 2018-01-05 | 东阿阿胶股份有限公司 | Excellent donkey-hide gelatin of a kind of odor qualities and preparation method thereof |
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Application publication date: 20160106 |