CN101601414A - A kind of monkshood extract and its production and use - Google Patents
A kind of monkshood extract and its production and use Download PDFInfo
- Publication number
- CN101601414A CN101601414A CNA2009100878320A CN200910087832A CN101601414A CN 101601414 A CN101601414 A CN 101601414A CN A2009100878320 A CNA2009100878320 A CN A2009100878320A CN 200910087832 A CN200910087832 A CN 200910087832A CN 101601414 A CN101601414 A CN 101601414A
- Authority
- CN
- China
- Prior art keywords
- monkshood
- extract
- monkshood extract
- standby
- pulverized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of efficient, low toxicity, low-residual, with environment and with other biological harmonious monkshood extract agricultural chemicals; take steps such as lixiviate, diacolation or refluxing extraction in leaching process, it all has far reaching significance to environmental protection and the pesticide resistance that solves the evil mite.Compare with pesticides prepared by chemistry, the present invention has following characteristics and advantage: because monkshood is the plant of occurring in nature, so its extract is easy to decompose under field conditions (factors), to environment and ecosystem safety on every side, do not have problem of environmental pollution; Monkshood extract is a kind of multicomponent mixture, and Tetranychus cinnabarinus is had a plurality of action sites, therefore is difficult for developing immunity to drugs; Experiment showed, that monkshood extract has significant miticidal effect, has the chemical pesticide characteristics of high efficiency; Extraction of active ingredients is simple in the monkshood, very helps carrying out suitability for industrialized production.
Description
Invention field
The present invention relates to a kind of plant extracts, particularly a kind of monkshood extract and its production and use.
Technical background
Phytophagous mites have individual little, breeding is fast, population density is big, cause harm and heavily wait characteristics, is the pest group that is difficult to prevent and treat that generally acknowledges.Tetranychus cinnabarinus (Tetranychus cinnabarinus) is subordinate to arachnids, Acariformes, Tetranychidae, is a kind of important harmful mite that endangers fruits and vegetables and hothouse plants.Chemical pesticide is to the pest mite class in the control agricultural production, and cover crop is injury-free and ensure that agricultural economy value has played important function.But because the long-term a large amount of chemical insecticides that use have produced a series of negative effect, as pest resistance increase, natural enemy quantity reduces, environmental pollution is serious, human health is impaired.Therefore, efficiently, wide spectrum, the acaricidal development of safety have become the task of top priority.The research of seeking insecticide active substance from plant provides new reactive compound for the novel pesticide research and development, it is is further researched and developed pesticide new variety have great significance.
Summary of the invention
The object of the invention is to disclose a kind of monkshood, and (Herba Polygoni Avicularis, Polygonaceae) extract the present invention also aims to disclose this preparation method of extract, the present invention also aims to disclose the purposes of this extract.
The present invention seeks to be achieved by the following scheme:
Monkshood extract of the present invention is by a kind of being prepared from the following method:
A. monkshood is pulverized, standby, with 2-8 volume chloroform soaking at room temperature doubly, lixiviate 2-4 time, each 2-6 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 2-8 volume chloroform doubly, diacolation extracts 2-5 time, or continuous backflow extracted 4-10 hour, filters, and merges extract, and concentrating under reduced pressure gets monkshood extract of the present invention.
Monkshood extract of the present invention is preferably as follows a kind of being prepared from the method:
A. monkshood is pulverized, standby, with 5 volumes chloroform soaking at room temperature doubly, lixiviate 3 times, each 3-5 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 5 volumes chloroform doubly, diacolation extracts 3 times, or continuous backflow extracted 7 hours, filters, and merges extract, concentrating under reduced pressure, monkshood extract of the present invention.
The invention described above monkshood extract can also be prepared as the monkshood extract, concrete grammar is: the monkshood extract that takes by weighing 0.001 weight portion, add 0.1-0.5 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 1-3mg/ml.
The invention described above monkshood extract can also be prepared as the monkshood extract, method for optimizing is: take by weighing the monkshood extract of 0.001 weight portion, add 0.3 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 2mg/ml.
Wherein, weight portion and parts by volume are the relation of g/ml among the above-mentioned monkshood extract preparation method.
The invention provides a kind of efficient, low toxicity, low-residual, with environment and with other biological harmonious novel monkshood extract agricultural chemicals, it all has far reaching significance to environmental protection and the pesticide resistance that solves the evil mite.Compare with pesticides prepared by chemistry, the present invention has following characteristics and advantage:
(1) because monkshood is the plant of occurring in nature, so its extract is easy to decompose under field conditions (factors), to environment and ecosystem safety on every side, do not have problem of environmental pollution.(2) monkshood extract is a kind of multicomponent mixture, and Tetranychus cinnabarinus is had a plurality of action sites, therefore is difficult for developing immunity to drugs.(3) experiment showed, that monkshood extract has significant miticidal effect, has the chemical pesticide characteristics of high efficiency.(4) extraction of active ingredients is simple in the monkshood, very helps carrying out suitability for industrialized production.
Following experimental example or embodiment are used to further specify but are not limited to the present invention.
The Tetranychus cinnabarinus determination of activity of killing of experimental example 1 monkshood extract is tested
1, experiment material
(1) preparation of monkshood extract: obtain different solvents (benzinum, chloroform and methyl alcohol) monkshood extract by embodiment 1 described method;
(2) above-mentioned three kinds of monkshood extracts are prepared the monkshood extract of 2mg/mL according to embodiment 2 described methods;
(3) preparation of Tetranychus cinnabarinus: Kidney bean (Vigna unguiculata) is planted in the nutritive cube (diameter 10cm, high 10cm), after bean seedlings grow six true leaves, Tetranychus cinnabarinus (T.viennensis) is raised in inoculation, temperature: 27 ± 2 ℃, relative moisture: 60%, illumination: 16h: 8h.
2, become the test of mite contact toxicity
The two-sided tape that 3cm is long is pasted on slide one end, throws off the top scraps of paper.With No. zero consistent, the brightly painted active one-tenth mite of writing brush picking size, its back is bonded on the two-sided tape, guarantee that mite foot, mouthpart and pedipalp are free movable, every 30-40 head.Be stained with slide with the inspection of binocular anatomical lens, reject inertia, injured and sticking underproof mite for the examination mite.Be placed on the filter paper that is soaked with the monkshood extract being stained with the slide of handling the examination mite, make for the examination mite to keep required humidity, move into indoor (27 ± 2 ℃) then.After 24 hours, under the binocular anatomical lens, touch the examination mite with writing brush, motionless person is death with the mite foot.Experiment repeats 3 times, and 1% Tween-80 solution is contrast.
3, deal with data
Use Abbott ' s formula calculation correction lethality, the difference between relatively respectively handling with the method for Duncan.Linear regression is tried to achieve according to the probit value analytical method.Finish test data variance and Duncan ' s check with the DPSv3.01 professional version.Lethality=dead borer population * 100%/total borer population; Corrected mortality=(handling lethality-contrast lethality) * 100% (100%-contrasts lethality).
4, experimental result
Three kinds of monkshood extracts become the contact toxicity of mite to Tetranychus cinnabarinus
Adopt the slide infusion process to measure the contact toxicity of three kinds of monkshood extracts, the results are shown in Table 1 Tetranychus cinnabarinus.When the monkshood extract concentration is 2mg/mL, monkshood methanol extract liquid and chloroform extracted solution show higher contact toxicity to Tetranychus cinnabarinus, in addition, the highest to the corrected mortality of Tetranychus cinnabarinus in three kinds of monkshood extracts as can be seen from the table with the monkshood chloroform extracted solution, reach 75.77%.
Three kinds of monkshood extracts of table 1 become the contact toxicity of mite to Tetranychus cinnabarinus
Annotate: in the table in the same column data same letter be expressed as the not remarkable of 5% level.
5, experiment conclusion
Experimental result shows that the monkshood chloroform extract shows higher contact toxicity to Tetranychus cinnabarinus.When the monkshood extract concentration reached 2mg/mL, the monkshood chloroform extracted solution showed comparatively ideal miticidal effect.
Embodiment all can realize above-mentioned effect below the present invention.
Embodiment
Embodiment 1: the preparation of monkshood extract of the present invention
Monkshood is pulverized, standby, with the chloroform soaking at room temperature of 5 times of weight, lixiviate 3 times, each 3-5 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention.
Embodiment 2: the preparation of monkshood extract of the present invention
Take by weighing the monkshood extract among the 0.001g embodiment 1, add the 0.3ml Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 2mg/ml.
Embodiment 3: the preparation of monkshood extract of the present invention
With the monkshood pulverizing, standby, add 3 volumes chloroform doubly, diacolation extracts 3 times, filters, and merges extract, and concentrating under reduced pressure gets monkshood extract of the present invention.
Embodiment 4: the preparation of monkshood extract of the present invention
With the monkshood pulverizing, standby, add 5 volumes chloroform doubly, continuous backflow was extracted 7 hours, filtered, and merged extract, and concentrating under reduced pressure gets monkshood extract of the present invention.
Claims (10)
1, a kind of monkshood extract is characterized in that this extract is prepared by a kind of method in the following method:
A. monkshood is pulverized, standby, with 2-8 volume chloroform soaking at room temperature doubly, lixiviate 2-4 time, each 2-6 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 2-8 volume chloroform doubly, diacolation extracts 2-5 time, or continuous backflow extracted 4-10 hour, filters, and merges extract, and concentrating under reduced pressure gets monkshood extract of the present invention.
2, monkshood extract as claimed in claim 1 is characterized in that this extract is prepared by a kind of method in the following method:
A. monkshood is pulverized, standby, with 5 volumes chloroform soaking at room temperature doubly, lixiviate 3 times, each 3-5 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 5 volumes chloroform doubly, diacolation extracts 3 times, or continuous backflow extracted 7 hours, filters, and merges extract, concentrating under reduced pressure, monkshood extract of the present invention.
3, monkshood extract as claimed in claim 1 or 2 is characterized in that this preparation method of extract also comprises the steps:
Take by weighing the monkshood extract of 0.001 weight portion, add 0.1-0.5 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 1-3mg/ml.
4, monkshood extract as claimed in claim 3 is characterized in that the step that this preparation method of extract also comprises is:
Take by weighing the monkshood extract of 0.001 weight portion, add 0.3 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 2mg/ml.
5,, it is characterized in that this method is a kind of in the following method as the preparation method of the arbitrary described monkshood extract of claim 1-4:
A. monkshood is pulverized, standby, with 2-8 volume chloroform soaking at room temperature doubly, lixiviate 2-4 time, each 2-6 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 2-8 volume chloroform doubly, diacolation extracts 2-5 time, or continuous backflow extracted 4-10 hour, filters, and merges extract, and concentrating under reduced pressure gets monkshood extract of the present invention.
6, the preparation method of monkshood extract as claimed in claim 5 is characterized in that this method is a kind of in the following method:
A. monkshood is pulverized, standby, with 5 volumes chloroform soaking at room temperature doubly, lixiviate 3 times, each 3-5 days, with leaching liquor difference concentrating under reduced pressure, merge, promptly get monkshood extract of the present invention;
B. monkshood is pulverized, standby, add 5 volumes chloroform doubly, diacolation extracts 3 times, or continuous backflow extracted 7 hours, filters, and merges extract, concentrating under reduced pressure, monkshood extract of the present invention.
7, the preparation method of monkshood extract as claimed in claim 5 is characterized in that this method also comprises the steps:
Take by weighing the monkshood extract of 0.001 weight portion, add 0.1-0.5 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 1-3mg/ml.
8, the preparation method of monkshood extract as claimed in claim 7 is characterized in that the step that this method also comprises is:
Take by weighing the monkshood extract of 0.001 weight portion, add 0.3 parts by volume Tween-80, stir, when extract with after tween combines fully, use distilled water diluting, being made into concentration is the monkshood extract of 2mg/ml.
9, as the application of the arbitrary described monkshood extract of claim 1-4 in the preparation miticide.
10, the application of plant monkshood in the preparation miticide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910087832A CN101601414B (en) | 2009-06-26 | 2009-06-26 | Monkshood extract, preparation method thereof and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910087832A CN101601414B (en) | 2009-06-26 | 2009-06-26 | Monkshood extract, preparation method thereof and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101601414A true CN101601414A (en) | 2009-12-16 |
| CN101601414B CN101601414B (en) | 2012-10-10 |
Family
ID=41467436
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910087832A Expired - Fee Related CN101601414B (en) | 2009-06-26 | 2009-06-26 | Monkshood extract, preparation method thereof and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101601414B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783095A (en) * | 2014-02-27 | 2014-05-14 | 江苏省激素研究所股份有限公司 | Traditional Chinese medicine pesticide |
| CN106472591A (en) * | 2016-09-22 | 2017-03-08 | 关慧明 | A kind of ecology insecticidal bactericide and preparation method thereof |
-
2009
- 2009-06-26 CN CN200910087832A patent/CN101601414B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103783095A (en) * | 2014-02-27 | 2014-05-14 | 江苏省激素研究所股份有限公司 | Traditional Chinese medicine pesticide |
| CN103783095B (en) * | 2014-02-27 | 2016-08-31 | 江苏省激素研究所股份有限公司 | A kind of Pesticide with Chinese medicinal material |
| CN106472591A (en) * | 2016-09-22 | 2017-03-08 | 关慧明 | A kind of ecology insecticidal bactericide and preparation method thereof |
| CN106472591B (en) * | 2016-09-22 | 2020-02-18 | 关慧明 | Ecological insecticidal bactericide and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101601414B (en) | 2012-10-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103392745B (en) | Environmentally-friendly pesticide synthesized by utilizing oleander extract and preparation method thereof | |
| CN104855412A (en) | Dispersible oil suspending agent containing spirotetramat and dinotefuran | |
| CN108041083B (en) | Plant source pesticide synergist and application thereof | |
| CN105613606A (en) | Plant-source aphicide prepared from Cinnamomum camphora (L.) Presl. essential oil | |
| CN101336644A (en) | Plant insecticide prepared by humulus and its preparing process horehound | |
| CN101601414B (en) | Monkshood extract, preparation method thereof and use thereof | |
| CN104705349B (en) | The preparation at white lotus parthenium extract different activities position and its application as acaricidal activity material | |
| CN101584349A (en) | Castor bean extractive and preparation method and application thereof | |
| CN101986844B (en) | Application of asarum extract in preventing and controlling agricultural mites | |
| CN112106789B (en) | Application of delphinium ethyl acetate extract in preventing and treating diamond back moth | |
| CN112471179B (en) | Application of filicina sibirica in pest and mite killing, biological control agent and preparation method thereof | |
| CN101411341A (en) | Pokeberry extract and novel use thereof | |
| CN101584348A (en) | A kind of inula flower extractive and its production and use | |
| CN104705350B (en) | A kind of white sand parthenium extract and its preparation method and application | |
| CN101584347A (en) | Dichroa febrifuga extractive and preparation method and application thereof | |
| CN101919416B (en) | Method for extracting insecticidal active material from traditional Chinese medicine feather cockscomb and application thereof | |
| CN103548884B (en) | Chinese pistache kills Tetranychus cinnabarinus agent and preparation method thereof | |
| Taha et al. | The first study for the Acaricidal activity of alcoholic extracts of Adiantum capillus-veneris and Funaria hygrometric against Argas persicus | |
| CN102578094B (en) | Use of (+)- epiafzelechin as pesticide | |
| Wang et al. | Acaricidal activities of Wikstroemia chamaedaphne extracts against Tetranychus urticae and Tetranychus cinnabarinus (Acari: Tetranychidae) | |
| CN100553456C (en) | A kind of cimicifuga foetida extract and new purposes thereof | |
| CN1281137C (en) | Application of yangtao plant resource in pesticide | |
| CN103651444A (en) | Pesticide combination | |
| CN105767024A (en) | Plant-sourced acaricide containing acalypha australis extract and preparation method thereof | |
| CN103548886B (en) | Use of Ferula bungeanaemulsion oil or microemulsion as insecticide and acaricide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121010 Termination date: 20130626 |