CN101596545B - Method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil - Google Patents
Method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil Download PDFInfo
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- CN101596545B CN101596545B CN2009100724089A CN200910072408A CN101596545B CN 101596545 B CN101596545 B CN 101596545B CN 2009100724089 A CN2009100724089 A CN 2009100724089A CN 200910072408 A CN200910072408 A CN 200910072408A CN 101596545 B CN101596545 B CN 101596545B
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Abstract
The invention provides a method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on the leaf face of morel to restore cadmium-polluted soil and relates to a method for restoring soil by morel, solving the problem that the existing method for restoring polluted soil by the morel has low biological mass, low restoring rate, long consumed time and hard mechanization operation and easily causes the secondary pollution of the soil. The method comprises the following steps: 1, morel seeds are cultured in the soil after being soaked in dioxan aminoethyl alcohol carboxylic ether; 2, morel seedlings which grow to four leaves are transplanted into cadmium-polluted soil, and then the dioxan aminoethyl alcohol carboxylic ether is sprayed and applied to the leaf face of the morel; and 3, the morel is harvested after being ripe. The method adopts leaf face spraying and applying, has simple operation and high mechanization degree, avoids the secondary pollution of the soil, enhances the growth rate of the morel, increases the extraction quantity of part of cadmium on the ground and has the advantage of high restoring rate; and the invention can realize high biological mass and the extraction of high cadmium in the soil by spraying and applying the dioxan aminoethyl alcohol carboxylic ether on the leaf face of the morel and can quicken the governing progress of cadmium pollution in the soil.
Description
Technical field
The present invention relates to a kind of method of black nightshade rehabilitating soil.
Background technology
Nearly ten years, soil pollution caused the public's extensive concern, and ever-increasing pollution has caused the large tracts of land soil can not farming and influenced human beings'health.And the most difficult with heavy metal pollution in the soil pollution, because of heavy metal pollution is that a class can not be degraded by biology, and along the pollutant of food chain enrichment, the ecological risk height is wherein especially with the harm maximum of mercury and cadmium.Traditional improvement method mainly adopts physics or chemical method, but has expense height, equipment complexity, to the repairing effect difference of large-area pollution and the shortcoming of secondary pollution, and is abandoned by people gradually; Compare the deficiency of traditional improvement technology, phytoremediation technology is low, simple to operate with cost, advantages of environment protection and gaining great popularity, but along with going deep into of research, the weak point of phytoremediation is also found by people gradually, at present the successful method of phytoremediation cadmium pollution soil method is the black nightshade recovery technique, but black nightshade repair have that the biomass that extracts plant is low, poor growth, repairing efficiency is long and be difficult for the mechanization operation and cause secondary pollution problem.So, be difficult to large-area applications in practice and be difficult to satisfy the demand of quick repairing polluted soil.
Summary of the invention
The present invention seeks to have in order to solve existing black nightshade rehabilitating soil method that biomass is low, repair rate is low, length consuming time and be difficult for the mechanization operation and easily cause secondary pollution problem, and provide a kind of method of spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil.
The method of spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil realizes according to the following steps: one, black nightshade seed is soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 1～20mg/kg and handles 20～30h, plant in Cd then
2+Concentration is in 0～0.28mg/kg soil, cultivates; Two, will grow black nightshade seedling replanting to 4 leaves to Cd
2+Concentration is in the soil of 0.29～100mg/kg, and the 5th day, the 20th day, the 40th day and the 60th day after transplanting is respectively the applying dioxan aminoethyl alcohol carboxylic ether of 1～20mg/kg to the black nightshade leaf surface spraying concentration; Three, treat to gather in after the black nightshade maturation, get final product the cadmium pollution in the rehabilitating soil.
The present invention utilizes plant growth regulator applying dioxan aminoethyl alcohol carboxylic ether (DA-6) that the hyperaccumulative plant black nightshade is carried out foliage-spray, makes the hyperaccumulative plant black nightshade accumulate high biomass; Because adopt the mode of foliage-spray in the present invention, with make the present invention have workable, expense is cheap, do not destroy soil physico-chemical property, do not cause the advantage of secondary pollution; The present invention uses the hyperaccumulative plant black nightshade will realize that high-biomass reaches the extraction to high cadmium in the soil, can improve the repair rate of soil greatly, also can quicken the process that Cadmium Pollution in Soils is administered.
The specific embodiment
The specific embodiment one: the method for present embodiment spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil realizes according to the following steps: one, black nightshade seed is soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 1～20mg/kg and handles 20～30h, plant in Cd then
2+Concentration is in 0～0.28mg/kg soil, cultivates; Two, will grow black nightshade seedling replanting to 4 leaves to Cd
2+Concentration is in the soil of 0.29～100mg/kg, and the 5th day, the 20th day, the 40th day and the 60th day after transplanting is respectively the applying dioxan aminoethyl alcohol carboxylic ether of 1～20mg/kg to the black nightshade leaf surface spraying concentration; Three, treat to gather in after the black nightshade maturation, get final product the cadmium pollution in the rehabilitating soil.
Soil is mixed by 10: 1 mass ratioes with the peat composed of rotten mosses by Harbin University of Science and Technology's flower nursery soil in the present embodiment step 1.
The mode of cultivating in the present embodiment all adopts open-air planting type.
Till the amount that at every turn sprays applying dioxan aminoethyl alcohol carboxylic ether in the present embodiment step 2 does not flow so that the positive and negative blade face of black nightshade is moistening.
The specific embodiment two: what present embodiment and the specific embodiment one were different is that soil picks up from the black earth of the degree of depth in 1～20cm in the step 1, and the basic fertility of soil is that effective potassium of the available phosphorus of the alkali-hydrolyzable nitrogen of 308.61mg/kg, 104.1mg/kg, 281.0mg/kg, 11.94% organic matter and concentration are the Cd of 0.28mg/kg
2+, pH is 7.0～8.0.Other step and parameter are identical with the specific embodiment one.
The specific embodiment three: what present embodiment was different with the specific embodiment one or two is that soil picks up from the black earth of the degree of depth in 4～5cm in the step 1.Other step and parameter are identical with the specific embodiment one or two.
The specific embodiment four: what present embodiment was different with the specific embodiment one or two is in the step 1 black nightshade seed to be soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 2～18mg/kg.Other step and parameter are identical with the specific embodiment one or two.
The specific embodiment five: what present embodiment was different with the specific embodiment one or two is in the step 1 black nightshade seed to be soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 15mg/kg.Other step and parameter are identical with the specific embodiment one or two.
The specific embodiment six: what present embodiment was different with the specific embodiment one or two is in the step 1 black nightshade seed to be soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 10mg/kg.Other step and parameter are identical with the specific embodiment one or two.
The specific embodiment seven: present embodiment and the specific embodiment four, five or six are different be in the step 1 processing time be 22～28h.Other step and parameter are identical with the specific embodiment four, five or six.
The specific embodiment eight: present embodiment and the specific embodiment four, five or six are different be in the step 1 processing time be 24h.Other step and parameter are identical with the specific embodiment four, five or six.
The specific embodiment nine: that present embodiment and the specific embodiment one, two, seven or eight are different is the Cd of soil in the step 1
2+Concentration is 0.1～0.2mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven or eight.
The specific embodiment ten: that present embodiment and the specific embodiment one, two, seven or eight are different is the Cd of soil in the step 1
2+Concentration is 0.15mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven or eight.
The specific embodiment 11: that present embodiment and the specific embodiment one, two, seven or eight are different is the Cd of soil in the step 1
2+Concentration is 0.05mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven or eight.
The specific embodiment 12: present embodiment and the specific embodiment nine, ten or 11 are different is that the concentration of applying dioxan aminoethyl alcohol carboxylic ether in the step 2 is 2～18mg/kg.Other step and parameter are identical with the specific embodiment nine, ten or 11.
The specific embodiment 13: present embodiment and the specific embodiment nine, ten or 11 are different is that the concentration of applying dioxan aminoethyl alcohol carboxylic ether in the step 2 is 5mg/kg.Other step and parameter are identical with the specific embodiment nine, ten or 11.
The specific embodiment 14: present embodiment and the specific embodiment nine, ten or 11 are different is that the concentration of applying dioxan aminoethyl alcohol carboxylic ether in the step 2 is 10mg/kg.Other step and parameter are identical with the specific embodiment nine, ten or 11.
The specific embodiment 15: that present embodiment and the specific embodiment one, two, seven, eight, 12,13 or 14 are different is the Cd of back soil in the step 2
2+Concentration be 10mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven, eight, 12,13 or 14.
The specific embodiment 16: that present embodiment and the specific embodiment one, two, seven, eight, 12,13 or 14 are different is the Cd of back soil in the step 2
2+Concentration be 25mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven, eight, 12,13 or 14.
The specific embodiment 17: that present embodiment and the specific embodiment one, two, seven, eight, 12,13 or 14 are different is the Cd of back soil in the step 2
2+Concentration be 50mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven, eight, 12,13 or 14.
The specific embodiment 18: that present embodiment and the specific embodiment one, two, seven, eight, 12,13 or 14 are different is the Cd of back soil in the step 2
2+Concentration be 100mg/kg.Other step and parameter are identical with the specific embodiment one, two, seven, eight, 12,13 or 14.
The specific embodiment 19: present embodiment and the specific embodiment 15,16,17 or 18 are different is that the water content that makes soil is 40%～95% to the soil management of feeding water.Other step and parameter are identical with the specific embodiment 15,16,17 or 18.
The specific embodiment 20: present embodiment and the specific embodiment 15,16,17 or 18 are different is that the water content that makes soil is 55% to the soil management of feeding water.Other step and parameter are identical with the specific embodiment 15,16,17 or 18.
The specific embodiment 21: what present embodiment was different with the specific embodiment 19 or 20 is that soil is fed water the employed water of management for not containing the water resource of cadmium ion.Other step and parameter are identical with the specific embodiment 19 or 20.
The specific embodiment 22: the method for present embodiment spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil realizes according to the following steps: one, black nightshade seed is soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 10mg/kg and handles 24h, plant in Cd then
2+Concentration is in the 0.1mg/kg soil, cultivates; Two, will grow black nightshade seedling replanting to 4 leaves to Cd
2+Concentration is in the soil of 10mg/kg, and the 5th day, the 20th day, the 40th day and the 60th day after transplanting is respectively the applying dioxan aminoethyl alcohol carboxylic ether of 10mg/kg to the black nightshade leaf surface spraying concentration; Three, treat to gather in the crops the cadmium pollution that gets final product in the rehabilitating soil after the black nightshade maturation; Wherein soil picks up from the black earth of the degree of depth in 10cm in the step 1, and the basic fertility of soil is that effective potassium of the available phosphorus of the alkali-hydrolyzable nitrogen of 308.61mg/kg, 104.1mg/kg, 281.0mg/kg, 11.94% organic matter and concentration are the Cd of 0.28mg/kg
2+, the pH value is 7.5.
Reduce significantly according to the soil pollutant cadmium content after the reparation of present embodiment method, the repair rate of soil strengthens the method that adopts foliage-spray simultaneously, has also avoided soil is caused secondary pollution.
The specific embodiment 23: present embodiment sprays application to the applying dioxan aminoethyl alcohol carboxylic ether of variable concentrations on the black nightshade blade face, and cadmium concentration (AC) and black nightshade ground cadmium extracted amount is as shown in table 1 in the spraying concentration of applying dioxan aminoethyl alcohol carboxylic ether (DA-6), the cadmium concentration in the soil, the black nightshade aerial part cadmium concentration/soil.
Table 1
DA-6-5 represents that the concentration of applying dioxan aminoethyl alcohol carboxylic ether is 5mg/kg in the table 1; DA-6-10 represents that the concentration of applying dioxan aminoethyl alcohol carboxylic ether is 10mg/kg; DA-6-15 represents that the concentration of applying dioxan aminoethyl alcohol carboxylic ether is 15mg/kg.
As can be seen from Table 1, under the black nightshade situation that cadmium concentration constantly raises on the ground, black nightshade is to the absorption Cd of aerial part
2+Ability is also in continuous improve.Pollution cadmium content in the soil reduces significantly like this, has repaired the pollution level of heavy metal-polluted soil.
When the cadmium concentration in the soil is 0.28mg/kg, foliage-spray applying dioxan aminoethyl alcohol carboxylic ether (is that the concentration of 5mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is that the concentration of 10mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is under the condition of 15mg/kg in the concentration of applying dioxan aminoethyl alcohol carboxylic ether), increased by 14.86%, 37.97% and 32.78% respectively through recording the black nightshade recovery rate.
When the cadmium concentration in the soil is 10mg/kg, foliage-spray applying dioxan aminoethyl alcohol carboxylic ether (is that the concentration of 5mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is that the concentration of 10mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is under the condition of 15mg/kg in the concentration of applying dioxan aminoethyl alcohol carboxylic ether), increased by 8.29%, 25.38% and 24.24% respectively through recording the black nightshade recovery rate.
When the cadmium concentration in the soil is 25mg/kg, foliage-spray applying dioxan aminoethyl alcohol carboxylic ether (is that the concentration of 5mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is that the concentration of 10mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is under the condition of 15mg/kg in the concentration of applying dioxan aminoethyl alcohol carboxylic ether), increased by 28.05%, 35.51% and 34.94% respectively through recording the black nightshade recovery rate.
When the cadmium concentration in the soil is 50mg/kg, foliage-spray applying dioxan aminoethyl alcohol carboxylic ether (is that the concentration of 5mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is that the concentration of 10mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is under the condition of 15mg/kg in the concentration of applying dioxan aminoethyl alcohol carboxylic ether), increased by 6.04%, 20.64% and 6.0% respectively through recording the black nightshade recovery rate.
When the cadmium concentration in the soil is 100mg/kg, foliage-spray applying dioxan aminoethyl alcohol carboxylic ether (is that the concentration of 5mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is that the concentration of 10mg/kg, applying dioxan aminoethyl alcohol carboxylic ether is under the condition of 15mg/kg in the concentration of applying dioxan aminoethyl alcohol carboxylic ether), increased by 13.84%, 20.95% and 15.60% respectively through recording the black nightshade recovery rate.
Claims (9)
1. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil is characterized in that the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil realizes according to the following steps:
One, black nightshade seed is soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 1～20mg/kg and handles 20～30h, plant in Cd then
2+Concentration is in 0～0.28mg/kg soil, cultivates; Two, will grow black nightshade seedling replanting to 4 leaves to Cd
2+Concentration is in the soil of 0.29～100mg/kg, and the 5th day, the 20th day, the 40th day and the 60th day after transplanting is respectively the applying dioxan aminoethyl alcohol carboxylic ether of 1～20mg/kg to the black nightshade leaf surface spraying concentration; Three, treat to gather in after the black nightshade maturation, get final product the cadmium pollution in the rehabilitating soil.
2. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 1, it is characterized in that soil picks up from the black earth of the degree of depth in 1～20cm in the step 1, the basic fertility of soil is that effective potassium of the available phosphorus of the alkali-hydrolyzable nitrogen of 308.61mg/kg, 104.1mg/kg, 281.0mg/kg, 11.94% organic matter and concentration are the Cd of 0.28mg/kg
2+, the pH value is 7.0～8.0.
3. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 1 and 2 is characterized in that in the step 1 black nightshade seed being soaked in the applying dioxan aminoethyl alcohol carboxylic ether that concentration is 2～18mg/kg.
4. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 3 is characterized in that the processing time is 22～28h in the step 1.
5. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 1 is characterized in that the Cd of soil in the step 1
2+Concentration is 0.1～0.2mg/kg.
6. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 5, the concentration that it is characterized in that applying dioxan aminoethyl alcohol carboxylic ether in the step 2 is 2～18mg/kg.
7. according to the method for claim 1,2,4 or 6 described spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil, it is characterized in that the Cd of soil in the step 2
2+Concentration be 10mg/kg.
8. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 7 is characterized in that the water content that makes soil is 40%～95% to the soil management of feeding water.
9. the method for spraying and applying dioxan aminoethyl alcohol carboxylic ether on leaf face of morel to restore cadmium-polluted soil according to claim 8 is characterized in that soil is fed water the employed water of management for not containing the water resource of cadmium ion.
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| CN101960956B (en) * | 2010-08-09 | 2012-02-08 | 农业部环境保护科研监测所 | Method for controlling cadmium accumulated in leaf vegetables in contaminated soils |
| CN104138897A (en) * | 2013-05-08 | 2014-11-12 | 中国科学院沈阳应用生态研究所 | Reinforcement restoration method using hyperaccumulation restoration plant for restoration of cadmium contaminated soil |
| CN104871850A (en) * | 2015-06-12 | 2015-09-02 | 上海交通大学 | Method for improving repairing efficiency of heavy metal polluted plants |
| CN107853096A (en) * | 2017-11-02 | 2018-03-30 | 成都金川田农机制造有限公司 | The method for planting of black nightshade |
| CN108380667A (en) * | 2018-02-06 | 2018-08-10 | 中国科学院华南植物园 | A method of it improving rehabilitation plant and absorbs Cadmium in Soil efficiency |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1724184A (en) * | 2004-07-23 | 2006-01-25 | 中国科学院沈阳应用生态研究所 | A kind of method of utilizing the plant of Solanaceae cadmium pollution soil repair |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724184A (en) * | 2004-07-23 | 2006-01-25 | 中国科学院沈阳应用生态研究所 | A kind of method of utilizing the plant of Solanaceae cadmium pollution soil repair |
Non-Patent Citations (2)
| Title |
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| T. Macek et al..ACCUMULATION OF CADMIUM BY HAIRY-ROOT CULTURES OF SOLANUM NIGRUM.《BIOTECHNOLOGY LETTERS》.1994,第16卷(第6期),第621-624页. * |
| Yuebing Sun et al..Effects of cadmium and arsenic on growth and metal accumulation of Cd-hyperaccumulator Solanum nigrum L..《Bioresource technology》.2008,(第99期),第1103-1110页. * |
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