CN101584299B - Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture - Google Patents
Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture Download PDFInfo
- Publication number
- CN101584299B CN101584299B CN200910064654XA CN200910064654A CN101584299B CN 101584299 B CN101584299 B CN 101584299B CN 200910064654X A CN200910064654X A CN 200910064654XA CN 200910064654 A CN200910064654 A CN 200910064654A CN 101584299 B CN101584299 B CN 101584299B
- Authority
- CN
- China
- Prior art keywords
- medium
- culture
- milligrams
- tissue
- rapid propagation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the field of rapid propagation and tissue culture method of plant, more particularly relates to a culture medium of Prunus serrulata Lindl. and a method of rapid propagation and tissue culture. The culture medium consists of a large number of elements, micro elements, ferric salt, organic components, sycrose, agar and a plant growth regulating agent, a large number of elements thereof comprise: ammonium nitrate, potassium sulfate, monopotassium phosphate, magnesium sulfate heptahydrate, calcium nitrate tetrahydrate and calcium chloride dehydrate; the ferric salt thereof comprises: ferrous sulfate heptahydrate and dihydrate sodium ethylene diamine tetracetate; the plant growth regulating agent is 6-benzyladenine and has concentration of 0.5-1.0 milligram/liter; other components are the same as MS culture medium. The pH value of the culture medium is 5.6-5.8. The culture medium of rapid propagation and tissue culture and the method of rapid propagation and tissueculture, provided by the invention, are simple, convenient and easily feasible aiming at characteristics of Prunus serrulata Lindl.. The tissue culture seedling is free from malformation, elongated i n internode, enhanced in propagation coefficient, short in propagation period, which is about 50 days, good in culture effect and high in survival rate.
Description
(1) technical field
The invention belongs to the tissue culture and rapid propagation method field of plant, particularly a kind of medium of red autumnal leaves oriental cherry and tissue culture and rapid propagation method.
(2) background technology
Oriental cherry, the rose family, Prunus are famous ornamencal flower and tree in China, in North China, the most of areas of East China, south China, central plain area and northeast, northwest has widely the tradition custom of planting, many cities are decided to be it " city tree city flower ".The red autumnal leaves oriental cherry is a mutation of magnificent oriental cherry, and abroad through seed selection for many years, its proterties is stable, and its flower is very big, pale red, polyphyll, long stalk is arranged, in Henan mid or late March to April, bloom the middle ten days, the open or floral leaf of Hua Xianye is spent big and gorgeous with putting.Opening up leaf the early spring is peony, and 5 to July, leaf was a shiny red, high temperature and rainy Lao Ye in season gradual change darkviolet, and leaf is big and thick, meets red leaves late fall and becomes salmon pink.
Present red autumnal leaves oriental cherry is with grafting and cottage propagation, but reproduction coefficient is little, and rooting rate is low.In existing group culturation rapid propagating technology document, do not find system's report of red autumnal leaves oriental cherry tissue culture and rapid propagation method as yet.If tissue-culturing rapid propagation medium and tissue culture and rapid propagation method with reference to other woody plants carry out tissue-culturing rapid propagation to the red autumnal leaves oriental cherry; Can occur tissue cultivating seedling take out the leave sheet do not stretch, the deformity, present lotus throneization, internode is short and small; Thereby cause growth rate slow; Growth coefficient is little, becomes the seedling cycle long, becomes seedling of poor quality.
(3) summary of the invention
The object of the present invention is to provide a kind of red autumnal leaves oriental cherry tissue-culturing rapid propagation medium and tissue culture and rapid propagation method, design to the characteristics of red oriental cherry, simple and easy to do, growth rate is fast, becomes the seedling cycle short, and tissue-culturing rapid propagation is effective, and survival rate is high.
The technical scheme that the present invention adopts is following:
A kind of red autumnal leaves oriental cherry tissue-culturing rapid propagation medium; Form by macroelement, trace element, molysite, organic principle, sucrose, agar and plant growth regulator; The composition of its trace element, organic principle and sucrose, agar and concentration are with the MS medium, and the composition of its macroelement and every liter content are following: 500～550 milligrams in ammonium nitrate, 520～570 milligrams of potassium sulphates; 150～170 milligrams of potassium dihydrogen phosphates; 350～400 milligrams of epsom salts, 750～800 milligrams of four water-calcium nitrates, 90～100 milligrams of calcium chloride dihydrates; Its molysite form and every liter content following: 28～56 milligrams of ferrous sulfate heptahydrates, 38～76 milligrams of two water sodium ethylene diamine tetracetates; Said plant growth regulator is a 6-benzyladenine, and concentration is 0.5～1.0 mg/litre; The pH value of said medium is 5.6～5.8.
The present invention also provides and has utilized said red autumnal leaves oriental cherry tissue-culturing rapid propagation medium to carry out the method for tissue-culturing rapid propagation; Comprise sterilization, the sterilization of red autumnal leaves oriental cherry axillary bud stem section, the inoculation of axillalry bud stem explants, enrichment culture, culture of rootage, refining seedling, the transplanting of said medium, wherein carry out the dark place reason during the enrichment culture.
Said dark place is managed in the inoculation back and was carried out in the 4th～6 day, and the dark place reason time is 7～10 days.
The sterilization of the sterilization of said medium, red autumnal leaves oriental cherry axillary bud stem section, the inoculation of axillalry bud stem explants, enrichment culture, culture of rootage, refining seedling, transplanting can be carried out with reference to the tissue culture and rapid propagation method of other woody plants, also can carry out according to following method:
The sterilization of medium is at 120～125 ℃, 1.06～1.08kg/cm
2Pressure under carry out 15min.
The sterilization of red autumnal leaves oriental cherry axillary bud stem section is: with the running water flushing, scrub each stem section with liquid detergent more earlier, at last with flowing water flushing 30 minutes; Take on the superclean bench and sterilize, volumetric concentration is 75% alcohol sterilization 30 seconds, and mass concentration is 1% NaC10 sterilization 10 minutes, aseptic water washing 1 time, and using mass concentration again is 0.5% NaC10 sterilization 10～13 minutes, aseptic water washing 3～4 times.The lateral bud that said red autumnal leaves oriental cherry axillary bud stem section is sprouted by the red autumnal leaves oriental cherry then 4～May obtains.
The condition of said enrichment culture, culture of rootage is 25 ± 2 ℃ of temperature, intensity of illumination 2000Lx, and light application time is 12 hours/day; About about 7～10 days, the explant axillary bud sprouting of inoculation.
Concrete, enrichment culture is every at a distance from 25～30 days, from cultivating clip axillary bud stem section on the aseptic bud of growing thickly that produces, the repeated inoculation step is carried out enrichment culture, on vial, covers black cloth and carries out the dark place reason.
Culture of rootage for height on the medium more than the 1.5cm, the tissue culture plant inoculation of robust growth carries out culture of rootage in root media.7～10 days adularescent tips of a root grow behind the general access root media, can form complete root system after 20 days.
Said refining seedling, the tissue cultivating seedling of transplanting to taking root refine seedling through 1～2 day half-open glass cap refining seedling and 3～5 days standard-sized sheet glass cap in the greenhouse; Take out tissue cultivating seedling wherein, clean the medium that root system adheres to clear water, plant into the cave dish, transplanting medium is that the peat composed of rotten mosses and perlite mass ratio are 3～4: 1 mixture; 10～15 days of transplanting initial stage keep 25～28 ℃ of temperature, and relative moisture remains on more than 90%.
The composition of used its macroelement of medium of culture of rootage and every liter content are following: 810～830 milligrams in ammonium nitrate, 930～960 milligrams in potassium nitrate, 210～230 milligrams of calcium chloride dihydrates, 175～195 milligrams of epsom salts; Said plant growth regulator is the equal amount of mixture of IBA and NAA, and concentration is respectively 0.05～0.1 mg/litre; Other compositions and concentration are formed with said fast breeding culture medium.
The present invention helps the adjustment of red autumnal leaves oriental cherry tissue-culturing rapid propagation to the MS medium of universality.Adjustment is mainly reflected in three aspects: an aspect is the adjustment of total ion concentration, and total ion concentration is reduced to below 1/2 of pervasive medium.Be N: P on the other hand
2O
5: K
2The adjustment of O is with N: P
2O
5: K
2O was adjusted into 2: 1 by 1: 6: 1: 4. the third aspect is NH
4 +-N/NO
3 -The adjustment of-N is with NH
4 +-N/NO
3 --N was adjusted into 1: 2 by 1: 3.5.This adjustment makes red autumnal leaves oriental cherry tissue cultivating seedling leaf malformation disappear vane extension.
Covering black cloth during the enrichment culture, to carry out the dark place reason be to utilize the adaptation reaction of plant under low light condition to improve length of internode, and it is short and small between the Sacura Festival to improve on the universality medium red autumnal leaves, the problem of newborn blade lotus throneization.
The inventive method is through culture of rootage, and rooting rate reaches more than 90%, transplants survival rate about 90% behind the refining seedling.
The present invention has following advantage with respect to prior art:
Red autumnal leaves oriental cherry tissue-culturing rapid propagation medium provided by the invention and tissue culture and rapid propagation method, to the characteristics of red oriental cherry, simple and easy to do.Tissue cultivating seedling does not have deformity, internode elongation, and reproduction coefficient improves.Breeding cycle is short, about 50 days.Culture effect is good, and survival rate is high.
(4) description of drawings
Fig. 1 utilizes MS medium and embodiment 1 method of quick propagating and cultivating to cultivate the red autumnal leaves oriental cherry of growth;
Fig. 2 utilizes embodiment 1 medium and cultural method to cultivate but enrichment culture is not done the red autumnal leaves oriental cherry of dark place reason growth;
Fig. 3 is the red autumnal leaves oriental cherry after the reason of dark place in the embodiment of the invention 1;
Fig. 4 trains the seedling of taking root for red autumnal leaves oriental cherry group in the embodiment of the invention 1;
Fig. 5 is the red autumnal leaves oriental cherry of transplant survival in the embodiment of the invention 1;
Fig. 6 is the Cheng Miao of red autumnal leaves oriental cherry in the embodiment of the invention 1.
(5) embodiment:
Below with specific embodiment technical scheme of the present invention is described, but protection scope of the present invention is not limited thereto:
Embodiment 1
Red autumnal leaves oriental cherry tissue-culturing rapid propagation culture medium preparation is by containing following substance classes in every liter and quality is dissolved in the distilled water formulated.
500 milligrams in ammonium nitrate, 520 milligrams of potassium sulphates, 150 milligrams of potassium dihydrogen phosphates, 350 milligrams of epsom salts, 750 milligrams of four water-calcium nitrates; 90 milligrams of calcium chloride dihydrates, 22.3 milligrams of four water manganese sulphates, 8.6 milligrams in zinc sulphate, 6.2 milligrams of boric acid, 0.25 milligram of Sodium Molybdate Dihydrate; 0.83 milligram of KI, 0.025 milligram of CoCL2,0.025 milligram of cupric sulfate pentahydrate, 28 milligrams of ferrous sulfate heptahydrates, 38 milligrams of two water sodium ethylene diamine tetracetates; 100 milligrams of inositols, 0.5 milligram in nicotinic acid, 0.5 milligram of puridoxine hydrochloride, 0.1 milligram of thiamine hydrochloride; 2 milligrams of glycine, 0.5 milligram of 6-benzyladenine, sucrose 30 grams, agar 6 grams.Sodium hydroxide with 1M is adjusted to 5.6 with medium pH.
The tissue-culturing rapid propagation of red autumnal leaves oriental cherry:
It is 8.5 centimetres that prepared culture medium is put into height, and bore is 5.5 centimetres a vial, 50 milliliters every bottle, cover tight bottle cap, and in 121 ℃, 1.06kg/cm
2It is for use after 15 minutes to sterilize.
Get the red autumnal leaves oriental cherry and the 4-5 month sprout branch then, be cut into and be with an axillary bud stem section, with the running water flushing, scrub each stem section with liquid detergent more earlier, at last with flowing water flushing 30 minutes.Take on the superclean bench and sterilize, volumetric concentration is 75% alcohol sterilization 30 seconds, and mass concentration is 1% NaC10 sterilization 10 minutes, aseptic water washing 1 time, and using mass concentration again is 0.5% NaC 10 sterilizations 10 minutes, aseptic water washing 3～4 times.
Explant after on superclean bench, will sterilizing is inoculated in the medium; Put it into that culturing room breeds and culture of rootage, condition of culture is 25 ± 2 ℃ of temperature, and cultivating light source is 28W straight pipe type power saving fluorescent lamp; Intensity of illumination 2000Lx, light application time is 12 hours/day.
After 25 days, clip axillary bud stem section on the aseptic bud of sprouting from medium of growing thickly is inoculated in the medium of new preparation, constantly shoot proliferation.The stem section of clip height more than 1.5cm, be inoculated in and carry out culture of rootage in the root media; In every liter of root media with 810 milligrams in ammonium nitrate; 930 milligrams in potassium nitrate; 210 milligrams of calcium chloride dihydrates, 175 milligrams of ammonium nitrate, potassium sulphate, potassium dihydrogen phosphate, epsom salt, four water-calcium nitrate, calcium chloride dihydrates that substitute in the fast numerous group of training medium of epsom salt; Be all the 6-benzyladenine in the above-mentioned medium of mixture replacing of IBA and NAA of 0.05 mg/litre with concentration.7～10 days adularescent tips of a root grow after inserting root media, can form complete root system after 20 days.
In order to break the lotus throneization of the newborn blade of tissue cultivating seedling, easy and increase the tissue cultivating seedling length of internode apace, shorten into the seedling cycle, during enrichment culture, on vial, cover black cloth and carry out the dark place reason.The dark place is managed in the inoculation back and was carried out in the 6th day, and the dark place reason time is 10 days.
The tissue cultivating seedling of taking root refines seedling through 2 days half-open glass cap refining seedling and 3 days standard-sized sheet glass cap in the greenhouse; Take out tissue cultivating seedling wherein; Clean the medium that root system adheres to clear water, plant the cave dish into 72 holes, transplanting medium is the peat composed of rotten mosses: the perlite mass ratio is 3: 1 a mixture.10～15 days of transplanting initial stage keep 25～28 ℃ of temperature, and relative moisture remains on more than 90%.Transplanting survival rate is 92%.
From Fig. 1-6, can know, adopt the present invention that medium and tissue culture and rapid propagation method are provided, the newborn blade of the red autumnal leaves oriental cherry tissue cultivating seedling of turning out is leaf normal, and the leaf look dark green, and attitude stretches, no lotus throne phenomenon.Tissue cultivating seedling behind acclimatization and transplants, robust growth, cane enriches, newborn blade red autumnal leaves oriental cherry characteristic is obvious.When enrichment culture does not carry out the dark place reason, can find the problem that exists internode short and small.
Embodiment 2
Red autumnal leaves oriental cherry tissue-culturing rapid propagation culture medium preparation: contain 550 milligrams in ammonium nitrate in every liter, 570 milligrams of potassium sulphates, 170 milligrams of potassium dihydrogen phosphates; 400 milligrams of epsom salts; 800 milligrams of four water-calcium nitrates, 100 milligrams of calcium chloride dihydrates, 56 milligrams of ferrous sulfate heptahydrates; 76 milligrams of two water sodium ethylene diamine tetracetates, 1.0 milligrams of 6-benzyladenines; Other compositions and concentration are with embodiment 1; Medium pH is adjusted to 5.8.Remove the dark place reason time in the breeding method and be 7 beyond the highest heavens, other are with embodiment 1, and survival rate is 90%.
Embodiment 3
Red autumnal leaves oriental cherry tissue-culturing rapid propagation culture medium preparation: contain 525 milligrams in ammonium nitrate in every liter, 545 milligrams of potassium sulphates, 160 milligrams of potassium dihydrogen phosphates; 375 milligrams of epsom salts; 775 milligrams of four water-calcium nitrates, 95 milligrams of calcium chloride dihydrates, 42 milligrams of ferrous sulfate heptahydrates; 57 milligrams of two water sodium ethylene diamine tetracetates, 0.8 milligram of 6-benzyladenine; Other compositions and concentration are with embodiment 1; Medium pH is adjusted to 5.7.In the breeding method except the peat composed of rotten mosses: the perlite mass ratio is 4: 1, and other are with embodiment 1, and survival rate is 87%.
Embodiment 4
Be 1 day except half-open glass cap refining seedling in the breeding method, standard-sized sheet glass cap refining seedling is 5 beyond the highest heavens, and other parts of the composition of medium and cultural method are with embodiment 1, and survival rate is 90%
Claims (6)
1. red autumnal leaves oriental cherry tissue-culturing rapid propagation medium; Be made up of macroelement, trace element, molysite, organic principle, sucrose, agar and plant growth regulator, it is characterized in that, the composition of its trace element, organic principle and sucrose, agar and concentration are with the MS medium; The composition of its macroelement and every liter content are following: 500～550 milligrams in ammonium nitrate; 520～570 milligrams of potassium sulphates, 150～170 milligrams of potassium dihydrogen phosphates, 350～400 milligrams of epsom salts; 750～800 milligrams of four water-calcium nitrates, 90～100 milligrams of calcium chloride dihydrates; Its molysite form and every liter content following: 28～56 milligrams of ferrous sulfate heptahydrates, 38～76 milligrams of two water sodium ethylene diamine tetracetates; Said plant growth regulator is a 6-benzyladenine, and concentration is 0.5～1.0 mg/litre; The pH value of said medium is 5.6～5.8.
2. utilize the said red autumnal leaves oriental cherry of claim 1 tissue-culturing rapid propagation medium to carry out the method for tissue-culturing rapid propagation; Comprise sterilization, the sterilization of red autumnal leaves oriental cherry axillary bud stem section, the inoculation of axillalry bud stem explants, enrichment culture, culture of rootage, refining seedling, the transplanting of said medium; It is characterized in that, carry out the dark place reason during the enrichment culture;
Said propagation is every at a distance from 25～30 days, and from cultivating clip axillary bud stem section on the aseptic bud of growing thickly that produces, the repeated inoculation step is carried out enrichment culture, on vial, covers black cloth and carries out the dark place reason;
Said culture of rootage for height on the medium more than the 1.5cm, the tissue culture plant inoculation of robust growth carries out culture of rootage in root media;
The condition of said propagation, culture of rootage is 25 ± 2 ℃ of temperature, intensity of illumination 2000Lx, and light application time is 12 hours/day;
The used medium of enrichment culture is a fast breeding culture medium described in the claim 1;
The composition of used its macroelement of medium of culture of rootage and every liter content are following: 810～830 milligrams in ammonium nitrate, 930～960 milligrams in potassium nitrate, 210～230 milligrams of calcium chloride dihydrates, 175～195 milligrams of epsom salts; Said plant growth regulator is the equal amount of mixture of IBA and NAA, and concentration is respectively 0.05～0.1 mg/litre; Other compositions and concentration are formed with said fast breeding culture medium.
3. the method for utilizing red autumnal leaves oriental cherry tissue-culturing rapid propagation medium to carry out tissue-culturing rapid propagation as claimed in claim 2 is characterized in that, said dark place is managed in the inoculation back and carried out in the 4th～6 day, and the dark place reason time is 7～10 days.
4. the method for utilizing red autumnal leaves oriental cherry tissue-culturing rapid propagation medium to carry out tissue-culturing rapid propagation as claimed in claim 2 is characterized in that, the sterilization of said medium is at 120～125 ℃, 1.06～1.08kg/cm
2Pressure under carry out 15min.
5. the method for utilizing red autumnal leaves oriental cherry tissue-culturing rapid propagation medium to carry out tissue-culturing rapid propagation as claimed in claim 2; It is characterized in that; The sterilization of said red autumnal leaves oriental cherry axillary bud stem section is: with the running water flushing, scrub each stem section with liquid detergent more earlier, at last with flowing water flushing 30 minutes; Take on the superclean bench and sterilize, volumetric concentration is 75% alcohol sterilization 30 seconds, and mass concentration is 1% NaClO sterilization 10 minutes, aseptic water washing 1 time, and using mass concentration again is 0.5% NaClO sterilization 10～13 minutes, aseptic water washing 3～4 times.
6. the method for utilizing red autumnal leaves oriental cherry tissue-culturing rapid propagation medium to carry out tissue-culturing rapid propagation as claimed in claim 2; It is characterized in that said refining seedling, the tissue cultivating seedling of transplanting to taking root refine seedling through 1～2 day half-open glass cap refining seedling and 3～5 days standard-sized sheet glass cap in the greenhouse; Take out tissue cultivating seedling wherein, clean the medium that root system adheres to clear water, plant into the cave dish, transplanting medium is that the peat composed of rotten mosses and perlite mass ratio are 3～4: 1 mixture; 10～15 days of transplanting initial stage keep 25～28 ℃ of temperature, and relative moisture remains on more than 90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910064654XA CN101584299B (en) | 2009-04-15 | 2009-04-15 | Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910064654XA CN101584299B (en) | 2009-04-15 | 2009-04-15 | Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101584299A CN101584299A (en) | 2009-11-25 |
| CN101584299B true CN101584299B (en) | 2012-11-28 |
Family
ID=41368897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910064654XA Expired - Fee Related CN101584299B (en) | 2009-04-15 | 2009-04-15 | Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101584299B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010144A (en) * | 2015-07-28 | 2015-11-04 | 福建世纪金花科技有限公司 | Double cherry tissue culture propagation method |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217534B (en) * | 2011-04-19 | 2012-07-25 | 南京林业大学 | Cerasus campanulata somatic cell embryogenesis method |
| CN104496616A (en) * | 2014-11-24 | 2015-04-08 | 柳州市天姿园艺有限公司 | Preparation method of cymbidium faberi soilless culture nutrient solution |
| CN104838900A (en) * | 2015-06-10 | 2015-08-19 | 苏州苏农园艺景观有限公司 | Method for cultivating red-leaf cherry blossom |
| CN104973959A (en) * | 2015-06-25 | 2015-10-14 | 中国科学院武汉植物园 | Sightseeing greenhouse succulents improvement matrix and preparation method thereof |
| CN105325291B (en) * | 2015-09-25 | 2017-08-11 | 江苏农林职业技术学院 | The method for tissue culture of F. pendula Bean |
| CN105494100A (en) * | 2015-12-30 | 2016-04-20 | 江汉大学 | Tissue culture and rapid propagation method of oriental cherries |
| CN106396832A (en) * | 2016-06-22 | 2017-02-15 | 殷佩琼 | A plant rooting liquid and a preparing method thereof |
| CN106538392B (en) * | 2016-12-08 | 2019-04-19 | 上海杉一植物科技有限公司 | A kind of white oriental cherry tissue culture and rapid proliferation method |
| CN114027191A (en) * | 2021-11-12 | 2022-02-11 | 中国科学院东北地理与农业生态研究所 | Amelanchier in-vitro plant differentiation culture medium and culture method thereof |
-
2009
- 2009-04-15 CN CN200910064654XA patent/CN101584299B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 孟月娥等.日本晚樱组培快繁技术研究.《中国农学通报》.2006,第22卷(第10期),第264-266页. * |
| 李艳敏等.‘红叶樱花’的组织培养和快速繁殖.《植物生理学通讯》.2008,第44卷(第6期),第1163页第3-4节. * |
| 王慧娟等.樱花组培苗的移栽技术研究.《河南农业科学》.2006,(第11期),第99-101页. * |
| 邹娜等.观赏樱花繁殖技术研究进展.《西南林学院学报》.2007,第27卷(第6期),第42-46页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010144A (en) * | 2015-07-28 | 2015-11-04 | 福建世纪金花科技有限公司 | Double cherry tissue culture propagation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101584299A (en) | 2009-11-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101584299B (en) | Culture medium of rapid propagation and tissue culture of Prunus serrulata Lindl. and method of rapid propagation and tissue culture | |
| CN102499090B (en) | Method for isolated culture of Haworthia succulent plants | |
| CN102577952B (en) | Tissue culturing method for quercus virginiana | |
| CN101578960B (en) | Method for reducing aerial root of tissue culture seedling of hydrangea | |
| CN106857256B (en) | The method for improving beautiful dew breeding potential based on callus induction Regeneration Ways | |
| CN102124954A (en) | Induced rapid propagation culture medium for somatic embryos of leaves in vitro of photinia x frasery | |
| CN100381045C (en) | Idesia polycarpa Idesia tissue culture method | |
| CN101347099B (en) | Method for quickly breeding Louisiana cypress in-vitro | |
| CN102010247B (en) | Nutrient solution for potato detoxified seedling reproduction | |
| CN102210267B (en) | Method for regenerating rose into complete plant | |
| CN104012417A (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN103098712B (en) | Davallia mariesii breeding method | |
| CN104396759B (en) | The method that ash tree tissue cultures is bred fast | |
| CN105532400A (en) | Pear tree cutting seedling-raising method | |
| CN109328959B (en) | Method for cultivating new variety of rhododendron | |
| CN103039366A (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| CN102057871B (en) | Method for breeding cold-resistant eucalypt Xiang eucalypt No.1 variety suitable for north latitude 26 to 30 degrees | |
| CN106718764A (en) | A kind of propagation method of jonquil | |
| CN105532475A (en) | Cultivation method of borneol cinnamomum comphora with high borneol content | |
| CN103651140B (en) | A kind of gametophytic method of rapid propagation in vitro short moon moss and substratum thereof | |
| CN112042540A (en) | Rapid propagation method of hydrangea macrophylla | |
| CN102577942A (en) | Tissue culture method of callistemon hybridus 'golden ball' | |
| CN101999318B (en) | Method for efficiently propagating Chinese ash by tissue culture | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN103843539B (en) | A kind of muehlewbeckia complera stem segment cuttage propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121128 Termination date: 20150415 |
|
| EXPY | Termination of patent right or utility model |