CN101580554B - Anoectochilus roxburghii polyose, applications thereof in pharmacy and preparation method of anoectochilus roxburghii polyose - Google Patents
Anoectochilus roxburghii polyose, applications thereof in pharmacy and preparation method of anoectochilus roxburghii polyose Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000003814 drug Substances 0.000 claims abstract description 4
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- 239000000706 filtrate Substances 0.000 claims description 59
- 238000001556 precipitation Methods 0.000 claims description 44
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Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides an anoectochilus roxburghii polyose product, a preparation method and medical applications thereof. The anoectochilus roxburghii polyose is extracted from the anoectochilus roxburghii, the molecular weight thereof is between 1000 and 80000 daltons, and the solid state thereof is grey white or white. The product can be developed into drugs for reducing blood sugar and blood fat, and a health product. The preparation method is reliable and has easy operation, is suitable for large-scale production, does not pollute environment and has low cost.
Description
Technical field
The present invention relates to anoectochilus roxburghii polyose, relate in particular to this polysaccharide and preparation method and its medicinal use.
Background technology
Herba Anoectochili roxburghii is called golden silkworm, metal and stone pine, the careless lotus of tree etc., be that the plant floral leaf that the orchid family is opened the lip Cymbidium opens the herb of lip orchid, sweet, the property of distinguishing the flavor of is flat, effect with clearing heat and cooling blood, removing dampness and detoxicating can be treated diseases such as hemoptysis of pulmonary tuberculosis, diabetes, ephritis, urocystitis, myasthenia gravis, seminal emission, rheumatic and rheumatoid arthritis.
Herba Anoectochili roxburghii has been rich in plurality of active ingredients such as polysaccharide, trace element, cardiac glycoside, amino acid, organic acid, steroidal compounds, flavonoid compound and alkaloid.The total sugar content of Herba Anoectochili roxburghii is more than 32%, and wherein reducing sugar content is about 7%, and oligosaccharide content is about 11%, and polysaccharide content is about 14%.Recent study shows that the water-soluble polysaccharide of Herba Anoectochili roxburghii has important physiologically active, such as anti-inflammatory effect.
Summary of the invention
First purpose of the present invention is to provide a kind of anoectochilus roxburghii polyose.
Second purpose of the present invention also is to provide a kind of preparation method of anoectochilus roxburghii polyose.
The 3rd purpose of the present invention is to provide the medicinal use of the anoectochilus roxburghii polyose that the present invention relates to.
The invention provides a kind of anoectochilus roxburghii polyose, is to open the lip Cymbidium per nnial herb Herba Anoectochili roxburghii from the orchid family to extract, and its molecular weight is 1000～80000 dalton, solid-state being white in color or canescence.
Anoectochilus roxburghii polyose of the present invention is prepared by following method:
A. water is carried the Herba Anoectochili roxburghii pulverizing medicinal materials, and extracting in water is got extracting solution;
B. alcohol precipitation carries out alcohol precipitation through filtering the back with ethanol with the extracting solution that obtains in the steps A, isolates throw out and gets the Herba Anoectochili roxburghii Crude polysaccharides;
C. precipitate with the Herba Anoectochili roxburghii Crude polysaccharides water-soluble after, filter, filtrate is with adjusting PH with base value to 9.0～14.0, and then adds quaternary ammonium salt precipitate in filtrate, leaves standstill, centrifugal, suction filtration is got filtrate;
D. the alcohol precipitation filtrate that suction filtration obtains in step C adding ethanol carries out alcohol precipitation, taking precipitate;
E. the throw out washed among the step D of salt dissolves with chloride soln, regulates pH value to 3.0～7.0, leaves standstill, and centrifugal, suction filtration is got filtrate;
F. the refining filtrate that will obtain in the step e through alcohol precipitation refining or with the macroporous resin column bed make with extra care the aqueous solution;
G. add ethanol in the aqueous solution that alcohol precipitation obtains in step F and carry out alcohol precipitation, the taking precipitate drying gets anoectochilus roxburghii polyose.
In above-mentioned preparation method, the water of steps A is put forward process and can be adopted 40 ℃～100 ℃ water logging to steep more than the 30min, and filtered filtration residue adds water at 40 ℃～100 ℃ to be extracted once again, and extraction time is more than the 30min, leaching extracted twice liquid.
Alcohol precipitation in described step B, D and G, can adopt volumetric concentration is that 95% ethanol carries out alcohol precipitation, and through leaving standstill, isolating throw out after centrifugal.
Among described step B, D, the G behind the alcohol precipitation alcohol final volume concentration be 30%～95%.
Quaternary ammonium salt among the described step C can be selected hexadecyl TMA (TriMethylAmine) bromide and oxyhydroxide, cetyl pyridinium, iodate N-trimethyl chitin quaternary ammonium salt or chitosan hydroxypropyl-trimethyl ammonium chloride for use.
The alkali that adjust pH is used among the described step C can be sodium hydroxide, calcium hydroxide, potassium hydroxide, sodium bicarbonate or ammoniacal liquor etc.
Muriate in the described step e can be sodium-chlor or Repone K.
The acid that adjust pH is used in the described step e is hydrochloric acid, phosphoric acid, citric acid or sulfuric acid etc.
The macroporous resin of using in the described step F is AB-8 type, X-5 type, H107 type, S-8 type, D3520 type, D4006 type, D4020 type, NKA-9 type, XAD-2 type, XAD-3 type, HP type, SIP1300 type, SIP1400 type, HPD100 type, HPD300 type, HPD600 type, M3 type, ABD-4 type, DM-130 type, D101 type, D201 type, D301 type, 1300-type, 1300-66 type, 100 types, 500 types or 600 type macroporous resins.
Alcohol precipitation in the described step F is refining to be after the filtrate in the step e is concentrated into 3～12 times of thick anoectochilus roxburghii polyose weight, the adding volumetric concentration is 95% ethanol, make that alcohol volume content reaches 30%～95% in the solution, be settled out anoectochilus roxburghii polyose, again with the water heating for dissolving formation aqueous solution of anoectochilus roxburghii polyose with 3～12 times of amounts.
Macroporous resin bed in the described step F is refining to be that the filtrate in the step e is gone up the macroporous resin column bed, and water is eluted to the effluent liquid water white transparency, merges effluent liquid, concentrates, filter, the aqueous solution.
The application of anoectochilus roxburghii polyose of the present invention in hypoglycemic, the reducing blood-fat of preparation, hepatic also is provided.
The present invention adopts modern scientific method, described anoectochilus roxburghii polyose is separated, purifies, and prepared anoectochilus roxburghii polyose product safety is nontoxic, reliable preparation process, raw material is easy to get, the reaction conditions gentleness, the yield height, purity is good, environmental protection, good stability, also very low to preparation equipment and place requirement, be suitable for large-scale industrial production.
Result according to pharmacology, pharmacodynamic study shows that the prepared anoectochilus roxburghii polyose product of the present invention has tangible effect to cardiovascular systems, can be developed into hypoglycemic, reducing blood-fat, protects the liver the class medicine.
Embodiment
Below by specific embodiment the present invention is described in further detail, to help understanding content of the present invention.
Embodiment 1: the macroporous resin pre-treatment
Big pore resin model of the present invention has AB-8 type, X-5 type, H107 type, S-8 type, D3520 type, D4006 type, D4020 type, NKA-9 type, XAD-2 type, XAD-3 type, HP type, SIP1300 type, SIP1400 type, HPD100 type, HPD300 type, HPD600 type, M3 type, ABD-4 type, DM-130 type, D101 type, D201 type, D301 type, 1300-type, 1300-66 type, 100 types, 500 types or 600 type macroporous resins.The macroporous resin treatment principle of these models is very similar with step, so be that example is simply introduced with X-5 type macroporous resin in the following embodiments:
The pre-treatment of resin: spend the night with water or ethanol swelling, wet method dress post with ethanol or acetone wash-out, to reclaiming no white precipitate in back or muddy getting final product, washes with water to there not being the alcohol flavor then, and alternately wash-out is 2～3 times.Also usable acid, alkali soak, and then are washed to neutrality.
Regeneration of resin: non-adsorptivity impurity generally washes with water to be removed; Adsorptivity impurity can be removed (NaOH or HCl) with certain density acid or alkali lye.Add a certain amount of ethanol during preservation.
Embodiment 2: the anoectochilus roxburghii polyose preparation
Get the Herba Anoectochili roxburghii medicinal material, pulverize, get Herba Anoectochili roxburghii powder 1.0kg, add water 10.0L, 80 ℃ add the heat soaking extraction, and extraction time is 3.0 hours, and cooling is filtered, and gets filtrate 1.; Filter residue adds water 8.0L, and 80 ℃ of heating are extracted, and extraction time is 2.0 hours, and cooling is filtered, and gets filtrate 2., merges twice filtrate and gets filtrate 15.5L.In order to extract fully, water temperature needs proper extension extraction time when low in this step.
Filtrate is concentrated into 7.0L, filters, and gets filtrate 6.7L.Filtrate is centrifugal, get supernatant liquor 6.0L, in supernatant liquor, add 95% ethanol (v%, following alcohol concn all refers to volumetric concentration), make the alcohol concn in the solution reach 70%, leave standstill, centrifugal, get precipitation, precipitation is that 95% ethanol and acetone are respectively washed twice with volumetric concentration, vacuum-drying gets Herba Anoectochili roxburghii Crude polysaccharides 242.31g.
The Herba Anoectochili roxburghii Crude polysaccharides of 200.00g is dissolved in the water of 2.0L, filter, filtrate is used the NaOH solution adjust pH to 12.5 of 10.0mol/L, and then to add mass concentration in filtrate be 5% CTAB (cetyl trimethylammonium bromide) solution, make the CTAB concentration in the solution reach 1.5%, leave standstill, centrifugal, get filtrate 2.3L.Filtrate adds 95% ethanol, makes the alcohol concn in the solution reach 70%, leaves standstill, and is centrifugal, get precipitation, precipitation is the dissolving of 5% NaCl solution with the 1.0L mass concentration, is 38% hydrochloric acid soln adjusting pH value to 5.5 again with mass concentration, leaves standstill, centrifugal, filter, get filtrate 0.8L.
Filtrate is gone up the macroporous resin column bed, and water is eluted to the effluent liquid water white transparency, effluent liquid 4.4L, be concentrated into 1.0L, filter, filtrate 0.8L.Filtrate adds 95% ethanol, makes the alcohol concn in the solution reach 70%, leaves standstill, and is centrifugal, gets precipitation, and precipitation respectively washes twice with 95% ethanol and acetone, and vacuum-drying gets anoectochilus roxburghii polyose 4.62g.
Assay:
1. polysaccharide is differentiated: phenolsulfuric acid reagent react (positive)
2. proterties: white powder
2. polysaccharide content: 92.94% (measuring) with the phenolsulfuric acid method
3. polysaccharide molecule flow measurement: 28961 dalton (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 3: the anoectochilus roxburghii polyose preparation
Get the Herba Anoectochili roxburghii medicinal material, Herba Anoectochili roxburghii powder 1.0kg is got in pulverizing, adds water 12.0L, and 90 ℃ of heating are extracted, and extraction time is 4.0 hours, and cooling is filtered, and gets filtrate 1.; Filter residue adds water 10.0L, and 90 ℃ of heating are extracted, and extraction time is 3.0 hours, and cooling is filtered, and gets filtrate 2., merges twice filtrate and gets filtrate 17.0L.
Filtrate is concentrated into 7.8L, filters, and gets filtrate 7.3L.Filtrate is centrifugal, get supernatant liquor 6.8L, in supernatant liquor, add 95% ethanol, make the alcohol concn in the solution reach 60%, leave standstill, centrifugal, get precipitation, precipitation respectively washes twice with 95% ethanol and acetone, and vacuum-drying gets Herba Anoectochili roxburghii Crude polysaccharides 254.22g.
The Herba Anoectochili roxburghii Crude polysaccharides of 200.00g is dissolved in the water of 3.0L, filters, filtrate is with the NaOH solution adjust pH to 13.0 of 10.0mol/L, and then the CTAB solution of adding 5% in filtrate, make the CTAB concentration in the solution reach 1.5%, leave standstill, centrifugal, get filtrate 2.8L.Filtrate adds 95% ethanol, makes the alcohol concn in the solution reach 60%, leaves standstill, and is centrifugal, gets precipitation, and precipitation is the dissolving of 5% Nacl solution with the 1.2L mass concentration, regulates pH value to 5.0 with 38% hydrochloric acid soln again, leaves standstill, and is centrifugal, and filtration gets filtrate 1.0L.
Filtrate is gone up the macroporous resin column bed, and water is eluted to the effluent liquid water white transparency, effluent liquid 5.0L, be concentrated into 1.2L, filter, filtrate 1.0L.Filtrate adds 95% ethanol, makes the alcohol concn in the solution reach 60%, leaves standstill, and is centrifugal, gets precipitation, and precipitation respectively washes twice with 95% ethanol and acetone, and vacuum-drying gets anoectochilus roxburghii polyose 4.55g.
Assay:
1. polysaccharide is differentiated: phenolsulfuric acid reagent react (positive)
2. proterties: white powder
2. polysaccharide content: 90.23% (measuring) with the phenolsulfuric acid method
3. polysaccharide molecule flow measurement: 27698 dalton (with reference to two appendix VH of pharmacopeia in 2005).
Embodiment 4: the anoectochilus roxburghii polyose preparation
Get the Herba Anoectochili roxburghii medicinal material, pulverize, get Herba Anoectochili roxburghii powder 1.0kg, add water 10.0L, 80 ℃ add the heat soaking extraction, and extraction time is 3.0 hours, and cooling is filtered, and gets filtrate 9.0L; Filter residue adds water 8.0L, and 80 ℃ of heating are extracted, and extraction time is 2.0 hours, and cooling is filtered, and gets filtrate 7.0L, merges twice filtrate and gets filtrate 16.0L.In order to extract fully, water temperature needs proper extension extraction time when low in this step.
Filtrate is concentrated into 7.0L, filters, and gets filtrate 6.5L.Filtrate is centrifugal, get supernatant liquor 6.0L, in supernatant liquor, add 95% ethanol (v%, following alcohol concn all refers to volumetric concentration), make the alcohol concn in the solution reach 70%, leave standstill, centrifugal, get precipitation, precipitation is that 95% ethanol and acetone are respectively washed twice with volumetric concentration, vacuum-drying gets Herba Anoectochili roxburghii Crude polysaccharides 253.63g.
The Herba Anoectochili roxburghii Crude polysaccharides of 200.00g is dissolved in the water of 2.0L, filter, filtrate is used the NaOH solution adjust pH to 12.5 of 10.0mol/L, and then to add mass concentration in filtrate be 5% CTAB (cetyl trimethylammonium bromide) solution, make the CTAB concentration in the solution reach 1.5%, leave standstill, centrifugal, get filtrate 2.5L.Filtrate adds 95% ethanol, makes the alcohol concn in the solution reach 70%, leaves standstill, and is centrifugal, get precipitation, precipitation is the dissolving of 5% NaCl solution with the 1.2L mass concentration, is 38% hydrochloric acid soln adjusting pH value to 5.5 again with mass concentration, leaves standstill, centrifugal, filter, get filtrate 1.1L.
Filtrate is concentrated into 0.7L, adds 95% ethanol, make that ethanol content reaches 70% in the solution, leave standstill, centrifugal, get precipitation.Precipitation adds 95% ethanol again with 1.0L water heating for dissolving, makes that ethanol content reaches 70% in the solution, leaves standstill, and is centrifugal, gets precipitation.Precipitation adds 95% ethanol again with 1.0L water heating for dissolving, makes that ethanol content reaches 70% in the solution, leaves standstill, and is centrifugal, gets precipitation, and precipitation respectively washes twice with 95% ethanol and acetone, and vacuum-drying must anoectochilus roxburghii polyose 4.61g.
Assay:
1. polysaccharide is differentiated: phenolsulfuric acid reagent react (positive)
2. proterties: pale powder
2. polysaccharide content: 91.61% (measuring) with the phenolsulfuric acid method
3. polysaccharide molecule flow measurement: 31722 dalton (with reference to two appendix VH of pharmacopeia in 2005).
Experimental example:
Test of pesticide effectiveness result
A, anoectochilus roxburghii polyose reduce the effect of mouse blood sugar
Get mouse and be divided into 5 groups at random, the administration group is respectively anoectochilus roxburghii polyose (get the anoectochilus roxburghii polyose that embodiment 2 makes, proterties is that white powder, polysaccharide content are 92.94%, polysaccharide molecular weight is 28961 dalton) 80,160,240mg/kg; Control group is a physiological saline, continuous 4d, animal fasting 2h before the last administration, 3h eye socket rear vein beard is got blood after the administration, use the determination of glucose oxidase serum glucose level, the results are shown in Table 1, by statistical procedures, show anoectochilus roxburghii polyose 160mg/kg and 240mg/kg successive administration 4d after glucose level be starkly lower than control group.
Table 1 anoectochilus roxburghii polyose reduces the effect of mouse blood sugar
Annotate: compare * P＜0.05, * P＜0.01 with the normal control group
B, anoectochilus roxburghii polyose prevention effect on hyperlipemia in mice
It is some to get Kunming mouse, is divided into 6 groups at random, as table 2 and table 3.Gastric infusion is 10 days continuously, and blank group gives physiological saline, and other groups give corresponding medicine respectively.With last administration pneumoretroperitoneum injection in 1 hour egg-nog 0.5ml/25g.The physiological saline of blank group abdominal injection 0.5ml.Posterior orbit was got blood (fasting be can't help water 12 hours) in 20 hours, and 2500 rev/mins of low-temperature centrifugations are got two indexs of supernatant serum measurement TC and TG.
The effect of the hypercholesterolemia of table 2 anoectochilus roxburghii polyose prevention mouse
Experimental result shows that dosage is that the anoectochilus roxburghii polyose group of 0.240g/kg is suitable with the effect of Simvastatin group, so anoectochilus roxburghii polyose has the effect of highly significant to the hypercholesterolemia to the prevention mouse.
The effect of table 3 anoectochilus roxburghii polyose prevention mouse triglyceride
Experimental result shows that dosage is that the anoectochilus roxburghii polyose group of 0.240g/kg is suitable with the effect of Simvastatin group, so anoectochilus roxburghii polyose has the effect of highly significant to the triglyceride to the prevention mouse.
The liver protection effect of C, anoectochilus roxburghii polyose
Get 50 of mouse, be divided into blank group, model group, the basic, normal, high dosage group of anoectochilus roxburghii polyose at random.Each sample sets mouse gastric infusion every day given the test agent, blank group and model group are all irritated stomach equal-volume distilled water, continuous 30 days.In testing the 31st day with each treated animal fasting overnight 16 hours, a control group and a sample sets are once injected on an empty stomach and are given 0.1%CCl
40.1mL/10g body weight, blank group abdominal injection equal-volume Viscotrol C, each sample sets continues to be tried thing.Fasting 12h before the blood sampling, the animal eye socket is got blood behind the 24h.Survey ALT, AST with automatic clinical chemistry analyzer, and win liver, fix, do check pathological section with 10% formaldehyde solution.
The liver protection effect of table 4 anoectochilus roxburghii polyose
The table 5 test mice result of histopathologic examination
The table 6 test mice result of histopathologic examination
Anoectochilus roxburghii polyose has the effect of protecting liver as can be seen from Table 4, and table 5 and table 6 also can embody anoectochilus roxburghii polyose and have tangible liver protection effect simultaneously.
Claims (11)
1. the preparation method of an anoectochilus roxburghii polyose, described anoectochilus roxburghii polyose is to open the lip Cymbidium per nnial herb Herba Anoectochili roxburghii from the orchid family to extract, its molecular weight 1000～80000 dalton, solid-state being white in color or grey, the preparation method comprises following process:
A. water is carried the Herba Anoectochili roxburghii pulverizing medicinal materials, and extracting in water is got extracting solution;
B. alcohol precipitation carries out alcohol precipitation through filtering the back with ethanol with the extracting solution that obtains in the steps A, isolates throw out and gets the Herba Anoectochili roxburghii Crude polysaccharides;
C. precipitate with the Herba Anoectochili roxburghii Crude polysaccharides water-soluble after, filter, filtrate is with adjusting PH with base value to 9.0～14.0, and then adds quaternary ammonium salt precipitate in filtrate, leaves standstill, centrifugal, suction filtration is got filtrate;
D. the alcohol precipitation filtrate that suction filtration obtains in step C adding ethanol carries out alcohol precipitation, taking precipitate;
E. the throw out washed among the step D of salt dissolves with chloride soln, regulates pH value to 3.0～7.0, leaves standstill, and centrifugal, suction filtration is got filtrate;
F. the refining filtrate that will obtain in the step e through alcohol precipitation refining or with the macroporous resin column bed make with extra care the aqueous solution;
G. add ethanol in the aqueous solution that alcohol precipitation obtains in step F and carry out alcohol precipitation, the taking precipitate drying gets anoectochilus roxburghii polyose.
2. preparation method as claimed in claim 1, it is characterized in that: it is to adopt 40 ℃～100 ℃ water logging to steep more than the 30min that the water in the described steps A is put forward process, filtered filtration residue adds water at 40 ℃～100 ℃ to be extracted once again, and extraction time is more than the 30min, leaching extracted twice liquid.
3. preparation method as claimed in claim 1, it is characterized in that: the alcohol precipitation in described step B, D and G, be that to adopt volumetric concentration be that 95% ethanol carries out alcohol precipitation, and through leaving standstill, isolating throw out after centrifugal, the final volume concentration of alcohol is 30%～95% behind the alcohol precipitation.
4. preparation method as claimed in claim 1 is characterized in that: the quaternary ammonium salt among the described step C is selected hexadecyl TMA (TriMethylAmine) bromide and oxyhydroxide, cetyl pyridinium, iodate N-trimethyl chitin quaternary ammonium salt or chitosan hydroxypropyl-trimethyl ammonium chloride for use.
5. preparation method as claimed in claim 1 is characterized in that: the muriate in the described step e is sodium-chlor or Repone K.
6. preparation method as claimed in claim 1 is characterized in that: the macroporous resin of using in the described step F is AB-8 type, X-5 type, H107 type, S-8 type, D3520 type, D4006 type, D4020 type, NKA-9 type, XAD-2 type, XAD-3 type, HP type, SIP1300 type, SIP1400 type, HPD100 type, HPD300 type, HPD600 type, M3 type, ABD-4 type, DM-130 type, D101 type, D201 type, D301 type, 1300-type, 1300-66 type, 100 types, 500 types or 600 type macroporous resins.
7. preparation method as claimed in claim 1, it is characterized in that: the alcohol precipitation in the described step F is refining to be after filtrate in the step e is concentrated into 3～12 times of thick anoectochilus roxburghii polyose weight, the adding volumetric concentration is 95% ethanol, make that alcohol volume content reaches 30%～95% in the solution, be settled out anoectochilus roxburghii polyose, again with the water heating for dissolving formation aqueous solution of anoectochilus roxburghii polyose with 3～12 times of weight.
8. preparation method as claimed in claim 1 is characterized in that: the macroporous resin bed in the described step F is refining to be that the filtrate in the step e is gone up the macroporous resin column bed, and water is eluted to the effluent liquid water white transparency, merges effluent liquid, concentrates, filter, the aqueous solution.
9. the anoectochilus roxburghii polyose of described method preparation one of among the claim 1-8, its molecular weight is 1000～80000 dalton, solid-state being white in color or canescence.
10. the application of the described anoectochilus roxburghii polyose of claim 9 in the medicine of preparation reducing blood-fat.
11. the application of the described anoectochilus roxburghii polyose of claim 9 in the preparation hepatic.
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| TWI379688B (en) * | 2009-10-05 | 2012-12-21 | Univ China Medical | Anoectochilus spp. polysaccharide extracts and pharmaceutical compositions for stimulating growth of advantageous bacteria, stimulating release of granulocyte colony-stimulating factor, modulating t helper cell type i, and/or modulating t helper cell typ |
| CN102552692B (en) * | 2012-01-19 | 2013-09-11 | 漳州瑞祥堂生物科技有限公司 | Anoectochilus formosanus compound alcohol-relieving and liver-protecting capsule and preparation method thereof |
| CN102768254B (en) * | 2012-06-19 | 2014-05-21 | 福建金草生物集团股份有限公司 | Method for establishing Anoectochilus roxburghii fingerprint, and Anoectochilus roxburghii fingerprint and Anoectochilus formosanus Hay fingerprint |
| CN105315381A (en) * | 2014-07-22 | 2016-02-10 | 漳州市亚热带园艺技术有限公司 | Anoectochilus roxburghii polysaccharide extraction and preliminary purifying method and preparation method for oral liquid of anoectochilus roxburghii polysaccharide |
| CN104404100B (en) * | 2014-10-13 | 2020-04-14 | 皖西学院 | Method for producing anoectochilus roxburghii polysaccharide by suspension culture cells |
| CN105085696A (en) * | 2015-07-13 | 2015-11-25 | 福建省亚热带植物研究所 | Method for extracting Anoectochilus roxburghii polysaccharides |
| CN107337741B (en) * | 2017-06-17 | 2019-07-19 | 福建中医药大学 | A kind of preparation method and application of beautiful flower bud Shorthairy Antenoron polyoses extract |
| CN107412254B (en) * | 2017-06-17 | 2020-06-26 | 福建中医药大学 | Application of anoectochilus formosanus polysaccharide extract |
| CN112759662B (en) * | 2021-01-18 | 2021-12-28 | 福建农林大学 | Separation and purification method of tremella polysaccharide |
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