CN101579375B - Extract of bupleurum, preparation method and application thereof - Google Patents
Extract of bupleurum, preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an extract of bupleurum and preparation method thereof, belonging to the field of traditional Chinese medicine. The preparation method is as follows: drying and grinding the stem and/or leaves of the bupleurum to obtain coarse powder of the bupleurum; adding 75-95% of alcohol solution with 8-10 times of weight for reflux extraction at the temperature of 75-85 DEG C for 1-3 times, carrying out extraction for 2-4h every time, combining extracting solution and filtering the extracting solution, recycling and concentrating ethanol until the relative density is 1.0-1.1; adding water with 8-10 times of volume into concentrated solution, placing the mixture for 12h and filtering the mixture, eluting the mixture with D101 macroporous resin column, collecting eluate and vacuum-drying the eluate at the temperature of 70 DEG C to obtain the extract powder of the bupleurum. The invention also discloses a pharmaceutical composition containing the extract of the bupleurum andapplication thereof in preparing drugs for treating liver and nerve diseases.
Description
Technical field
The invention belongs to a kind of Chinese medicine extract, be specifically related to a kind of Radix Bupleuri extract and preparation method thereof and pharmaceutical composition that contains this Radix Bupleuri extract and the application on preparation treatment liver, biliary diseases medicine.
Background technology
Cholestatic hepatitis belongs to traditional Chinese medical science jaundice category, can be caused by multiple reason, and its main feature is that hepatic bile is retarded by silt.Primary disease sickness rate height, prognosis is relatively poor, has a strong impact on people's work capacity and quality of life.
Cholestatic hepatitis is divided into acute and chronic two types by its clinical manifestation.But the general spontaneous recovery of acute cholestatic hepatitis, and chronic cholestatic hepatitis such as untimely treatment, part patient will develop into hepatitis interstitialis chronica, liver failure and hepatocarcinoma.
Doctor trained in Western medicine thinks that the pathogenesis of cholestatic hepatitis is because cholate forms and turns round bad, the moisture that makes bile emulsifying and enter in the fine bile duct reduces, cause bile to be difficult for discharging, bile forms the gallbladder bolt and cholestasis takes place in fine bile duct, common drug has glucocorticoid, phenobarbital, ursodesoxycholic acid, secretin, potenlin etc., but prolonged application can produce stronger side effect.
The glucocorticoid of super physiological amount has multiple pharmacological effect such as infection, antiallergic and inhibition immunoreation, is often applied to treat all kinds of inflammatory conditions.But unsuitable use or long-term heavy dose of use can cause multiple untoward reaction and complication, as hypercortisolism, bring out ulcer, osteoporosis, steroid diabetes etc., even threat to life; Sequela such as phenobarbital uses separately dizziness, drowsiness are taken for a long time and can be produced drug resistance, and drug withdrawal syndrome can appear in dependency and retention toxicosis after the drug withdrawal; The untoward reaction of ursodesoxycholic acid has symptoms such as constipation, diarrhoea, allergy, headache; Potenlin (Potenline) is glycyrrhizic acid (glycyrrhizin) preparation; its main component is glycyrrhizic acid monoammonium, L-cysteine and glycine; have multiple pharmacological effect such as jaundice eliminating, antiinflammatory, antiallergic, protection hepatocyte, promotion bilirubin metabolism, the clinical treatment of diseases such as viral hepatitis, epidemic hemorrhagic fever that are used for.The potenlin life-time service can cause symptoms such as severe allergic reaction, hypokalemia paralysis, digestive tract paralysis, gingival hemorrhage, lactogenic, mental symptom.
The mechanism of treatment by Chinese herbs cholestatic hepatitis is by increasing the hepatic bile flow, promoting what bile secretion and lax Oddi's sphincter were realized.Chinese medicine can pass through the effect of many target spots of multipath too many levels multisystem, and the body internal and external environment is coordinated, the performance promoting the function of the gallbladder to alleviate jaundice, protect the liver, immunomodulating, antiinflammatory and antiviral effect, essentially no untoward reaction, medical treatment cost is relatively low.Chinese medicine preparation with its determined curative effect, can regulate advantages such as allomeric function, toxic and side effects be little, accepted extensively and use by the patient.But up to the present, the preparation listing of the Chinese medicine and the natural drug of more satisfactory treatment cholestatic hepatitis is not arranged as yet.
Summary of the invention
The object of the invention is to provide a kind of Radix Bupleuri extract, and this extract has good therapeutic effect for hepatopathy.
The present invention's second purpose provides the preparation method of above-mentioned Radix Bupleuri extract.
The present invention's the 3rd purpose provides the pharmaceutical composition that contains above-mentioned Radix Bupleuri extract.
The present invention's the 4th purpose provides the application of above-mentioned Radix Bupleuri extract on the treatment hepatopathy.
Realize that technical scheme of the present invention is as follows:
A kind of Radix Bupleuri extract is prepared as follows:
(1) with Radix Bupleuri, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li, stem and/or leaf drying and crushing, the Radix Bupleuri coarse powder; 75～95% alcoholic solutions that add 8～10 times of weight reflux, extract, 1～3 time in 75～85 ℃ was extracted 2～4 hours at every turn, and merge extractive liquid, filters, and reclaimed ethanol and concentrated, and being concentrated into relative density is 1.0-1.1;
(2) in concentrated solution, add the water of 8～10 times of volumes, placed 12 hours, filter, obtain filtrate;
(3) filtrate that step (2) is obtained is by D101 type macroporous resin column, and flow velocity is 2-5ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain the Radix Bupleuri extract powder.
Described alcoholic solution is an alcoholic solution.
The preparation method of above-mentioned Radix Bupleuri extract, carry out according to following steps:
(1) with Radix Bupleuri, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li, stem and/or leaf drying and crushing, the Radix Bupleuri coarse powder; 75～95% alcoholic solutions that add 8～10 times of weight reflux, extract, 1～3 time in 75～85 ℃ was extracted 2～4 hours at every turn, and merge extractive liquid, filters, and reclaimed ethanol and concentrated, and being concentrated into relative density is 1.0-1.1;
(2) in concentrated solution, add the water of 8～10 times of volumes, placed 12 hours, filter, obtain filtrate;
(3) filtrate that step (2) is obtained is by D101 type macroporous resin column, and flow velocity is 2-5ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain the Radix Bupleuri extract powder.
The pharmaceutical composition that contains above-mentioned Radix Bupleuri extract, described pharmaceutical composition are that the Radix Bupleuri extract of effective dose mixes with adjuvant commonly used on the pharmaceutics, make acceptable forms on the pharmaceutics.
Radix Bupleuri extract of the present invention can add various conventional adjuvant required when preparing different dosage form, be prepared into any peroral dosage form commonly used as disintegrating agent, lubricant, binding agent etc. with the method for Chinese medicinal of routine, as pill, powder, tablet, capsule, oral liquid etc.
Described pharmaceutical composition contains the component of following percentage by weight: above-mentioned Radix Bupleuri extract 50-75%; PEG-400 15-60%; Glycerol 0.5-25%; Tween 80 1-15%; The preferred soft capsule of dosage form.
The application of above-mentioned Radix Bupleuri extract on preparation treatment liver disease drug.
The application of above-mentioned Radix Bupleuri extract on preparation treatment cholestatic hepatitis, cholecystitis or icterohepatitis medicine.
The application of above-mentioned Radix Bupleuri extract pharmaceutical composition on preparation treatment liver disease drug.
The application of above-mentioned Radix Bupleuri extract pharmaceutical composition on preparation treatment cholestatic hepatitis, cholecystitis or icterohepatitis medicine.
The main flavone compound that the said extracted thing contains in this extract as can be known through following methods analyst.
(1) get 9.9 kilograms of stem of Radix Bupleuri and leaf parts and be ground into coarse powder (20 order), 10 times of amount 85% alcohol refluxs twice, each 1 hour, merge extractive liquid, was concentrated into into rare extractum.Rare extractum is used petroleum ether (60～90 ℃) and ethyl acetate extraction successively, reclaims ethyl acetate, gets extractum.
(2) separating extractum separates with polyamide column chromatography, with water, 10% ethanol, 30% ethanol, 50% ethanol, 70% ethanol, 95% ethanol gradient elution, one bottle of collection of every 500ml, the polyamide film inspection is known, merge the close stream part of composition, obtain seven solvent elution parts, be respectively Fr.I, Fr.II, Fr.III, Fr.IV, Fr.V, Fr.VI, Fr.VII.Fr.II is concentrated, has yellow solid to separate out, filter yellow solid, the solution concentration after filtering is got extractum 2.71g, yellow solid is with ethyl alcohol recrystallization, Compound I I (236mg).Extractum is further purified separation with silica gel column chromatography, with chloroform-methanol-water gradient elution, get Compound I (10mg), Compound I I (40mg), Fr.V separates with Sephadex LH-20 column chromatography, methanol-water eluting with 1: 1, get compound IV (32mg) and chemical compound V (22mg), Fr.VI concentrates, and has yellow solid to separate out, filter yellow solid, yellow solid separates through Sephadex LH-20 column chromatography, uses methanol-eluted fractions, gets compound III (39mg) and chemical compound V (10mg).Fr.VII separates through Sephadex LH-20 column chromatography, uses methanol-eluted fractions, gets compound VI (32mg).
This experiment separates from the Radix Bupleuri stem and leaf and has obtained six chemical compounds, has identified the structure of these six chemical compounds, and they are 5,7,4 respectively '-trihydroxy-3 '-methoxyflavonol-3-O-rutinoside (I); 5,7,3 ', 4 '-tetrahydroxyflavonol-3-O-rutinoside (II); Isorhamnetin (III); 5,7,3 ', 4 '-tetrahydroxyflavonol-3-O-α-L-arabinofuranosyl glycosides (IV); Quercetin (V); Laccaic acid (VI).More than six chemical compounds be all from this plant, to separate first and obtain, wherein compound VI obtains for separating from the Bupleurum platymiscium first, also is to separate for the first time to obtain anthraquinone class composition from the Bupleurum plant.
Radix Bupleuri described in the present invention, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.L i, this strain Umbelliferae Bupleurum plant, identify that through the drug inspection office, Qinghai Province medical material is a Radix Bupleuri parvifolii, outward appearance is similar to Radix Bupleuri, its root pitchy has more sparse band and difference with it, mainly is distributed in provinces and regions such as Hebei, Shanxi, Shaanxi, Henan, Gansu, Qinghai, Inner Mongol.
Radix Bupleuri parvifolii is an oblong, and is how crooked, the tool branch that has.Long 5～10cm, diameter 0.2～0.8cm.The surface pitchy, coarse, bossed supporting root trace.Hole skin is not obvious.Many tool 2～5 branches of root head, there are residual stem foot and phyllopodium in the top; Downside tool two row are tuberculate adventitious bud.Matter is crisp, frangibility, and section shows sheet-like fiber or slightly smooth, skin zone's light brown, woody part is faint yellow, is radial, feeble QI perfume (or spice), mildly bitter flavor suffering.
Discrimination method: (1) this product cross section: phellem layer is a row cell surplus in the of 10～40, and the cork cell is flat, and major diameter is about 4 times of minor axis.The phellem layer inboard is 3～5 layers of tangential parenchyma cell that prolongs, and 5～10 in oil pipe is arranged, and interrupted the arrangement is a ring.Phloem is narrower, and wherein secretory tissue is more, includes yellow oil.Cambium layer is formed successive ring or not obvious by 3～5 layers of flat little parenchyma cell.Xylem vessel is radial arrangement.The wood strand broad, interfascicular is organized narrower; Wood fiber group is distributed in the outside of xylem more, is dispersed in or intermittently arranges ring formation.This product rhizome has marrow, and there is oil pipe at nearly xylem place.
Advantage that the present invention has and beneficial effect: (1) the present invention has therapeutical effect preferably for cholestatic hepatitis, cholecystitis, icterohepatitis etc.; (2) the present invention derives from the natural plants Chinese crude drug, to people's environmental sound; (3) preparation method of the present invention is simple, and cost is low.
The specific embodiment
Embodiment 1-3 is the preparation method of Radix Bupleuri extract, and embodiment 4-6 is the preparation of the soft capsule of pharmaceutical composition of the present invention, and embodiment 7 is the pharmacodynamic experiment of Radix Bupleuri extract of the present invention.
Embodiment 1
(1) stem and leaf 3000 grams with Radix Bupleuri (Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.L i) are dried and crushed into about 20 orders, 85% ethanol that adds 9 times of weight reflux, extract, 3 times in 85 ℃, the each extraction 3 hours, merge extractive liquid,, filter, reclaim ethanol and concentrated, being concentrated into relative density is 1.0-1.1;
(2) water of 9 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 5ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 600g.
Embodiment 2
(1) stem and the leaf 3000g with Radix Bupleuri is dried and crushed into about 20 orders, and 95% ethanol that adds 8 times of weight reflux, extract, 2 times in 80 ℃ was extracted 4 hours at every turn, and merge extractive liquid, filters, and reclaims ethanol and concentrates, and being concentrated into relative density is 1.0-1.1;
(2) water of 10 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 2ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 459g.
Embodiment 3
(1) stem and the leaf 3000g with Radix Bupleuri is dried and crushed into 20 orders, and 75% ethanol that adds 10 times of weight reflux, extract, 2 times in 75 ℃ was extracted 3 hours at every turn, and merge extractive liquid, filters, and reclaims ethanol and concentrates, and being concentrated into relative density is 1.0-1.1;
(2) water of 8 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 4ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 735g.The preparation of embodiment 4 medicinal composition soft capsule agent of the present invention
Get the Radix Bupleuri extract 447g that embodiment 1 makes, PEG-400625.8g, Tween 80 23g, glycerol 57.7g mix homogeneously make capsule 's content; Rubber liquid gets by 42% gelatin, 20% glycerol, 37% water and 1% pigment brilliant black are mixed, is pressed into soft capsule in colloid mill.The heavy 0.50g of every capsules content, content is tan thick liquid, gas perfume (or spice), bitter in the mouth.
The preparation of embodiment 5 medicinal composition soft capsule agent of the present invention
Get the Radix Bupleuri extract 224g of embodiment 1 system, PEG-400358.4g, Tween 80 179.2g, glycerol 4.5g mix homogeneously, rubber liquid and preparation method thereof are pressed into soft capsule at last with embodiment 4 in colloid mill.The heavy 0.50g of every capsules content, content is tan thick liquid, gas perfume (or spice), bitter in the mouth.
The preparation of embodiment 6 medicinal composition soft capsule agent of the present invention
Get the Radix Bupleuri extract 650g of embodiment 3 systems, PEG-400270g, Tween 80 30g, glycerol 50g mix homogeneously, rubber liquid and preparation method thereof are pressed into soft capsule at last with embodiment 4 in colloid mill.The heavy 0.50g of every capsules content, content is tan thick liquid, gas perfume (or spice), bitter in the mouth.
Embodiment 7 Radix Bupleuri extracts of the present invention are to the choleretic effect of isothiocyanic acid-a-naphthalene ester (ANIT) rat model
ANIT is a kind of indirect hepatotoxic agent, mainly damages the stones in intrahepatic bile duct epithelial cell, causes that capillary tube hypertrophy and interlobular bile duct produce inflammation on every side, thereby causes bile duct obstruction, forms tangible obstructive jaundice, hyperbilirubinemia and bile secretion occur and reduces.
1), is subjected to the reagent thing
The Radix Bupleuri extract of embodiment 2 preparations; Positive drug is the Warsaw Livonal, German Warsaw pharmaceutical factory, production code member 10500.
2), animal
The Wister rat, 270-330g, male, the II level is purchased the animal center in Chinese biological goods calibrating institute.
3), experimental technique
Get the Wister rat, be divided into blank group at random, model group, the positive drug group (0.54/only/day), Radix Bupleuri extract powder consumption is divided into two dosage groups, high dose group 0.0324g//day, low dose group 0.0162g//day, 8 every group, be subjected to the reagent thing all to use water dissolution, each organizes rat gastric infusion every day 2 times, each 1ml, a continuous week.Blank group and model group are given the normal saline of equal volume.Irritate stomach after 3 days, except that the blank group, the equal lumbar injection 4%ANIT of all the other each treated animals vegetable oil solution carries out modeling by 0.1875ml/100g, continues gastric infusion according to dosage after the modeling.Before experiment finished, the animal fasting be can't help water 12 hours.Behind the last administration 1h, (ketamine: diazepam=1: 1) 0.25ml/100g anaesthetizes rat, opens abdomen and separates ductus choledochus with compound anesthetic, in nearly liver end sealing, liver end ligation far away is cut a V-shape breach at closed section towards the liver direction, inserts the plastic tube that bore is about 0.6mm, ligation is fixed, and draw stomach wall, bile picks up counting after flowing out, count per hour bile flow, the results are shown in Table 1.According to total bilirubin determination reagent kit (available from the BeiJing ShouYi clinical Medicine Science Center) time-and-motion study total bilirubin (TBIL) value, the results are shown in Table 2.
The rats'liver that ANIT brings out is damaged similar people's intrahepatic cholestasis pathological changes.By table 1 and 2 as can be seen, animal intrahepatic cholestasis pathological changes takes place, the modeling success.The T assay that records data shows that each group is compared with blank group, and significant difference (P＜0.01) is arranged.Compare with model group, Radix Bupleuri extract of the present invention has tangible promoting the function of the gallbladder to alleviate jaundice effect, improves the rat bile secretory volume, and can reduce the content of TBIL in the bile.High low dosage is compared, and high dose can significantly improve the rat bile secretory volume.
Table 1 Radix Bupleuri extract of the present invention is to the influence of rat bile secretory volume
P＜0.01, each group compares with the blank group, and significant difference is arranged.
Table 2 Radix Bupleuri extract of the present invention is to the influence of TBIL in the rat bile
Unit: g
| Blank | Model | Positive | High dose | Low dosage | |
| Meansigma methods | 0.027 | 0.243 | 0.24 | 0.208 | 0.199 |
| Standard deviation | 0.003 | 0.027 | 0.018 | 0.023 | 0.035 |
P＜0.01, each group and blank group contrast have significant difference.
Claims (3)
1. the preparation method of a Radix Bupleuri extract is characterized in that, matrix preparation technology is as follows:
(1) stem and leaf 3000 grams with Radix Bupleuri (Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li) are dried and crushed into 20 orders, 85% ethanol that adds 9 times of weight reflux, extract, 3 times in 85 ℃, the each extraction 3 hours, merge extractive liquid,, filter, reclaim ethanol and concentrated, being concentrated into relative density is 1.0-1.1; Described Radix Bupleuri, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li, it is Umbelliferae Bupleurum plant, identify that through the drug inspection office, Qinghai Province medical material is a Radix Bupleuri parvifolii, outward appearance is similar to Radix Bupleuri, its root pitchy has more sparse band and distinguishes with Radix Bupleuri;
(2) water of 9 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 5ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 600g.
2. the preparation method of a Radix Bupleuri extract is characterized in that, matrix preparation technology is as follows:
(1) stem and the leaf 3000g with Radix Bupleuri is dried and crushed into 20 orders, and 95% ethanol that adds 8 times of weight reflux, extract, 2 times in 80 ℃ was extracted 4 hours at every turn, and merge extractive liquid, filters, and reclaims ethanol and concentrates, and being concentrated into relative density is 1.0-1.1; Described Radix Bupleuri, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li, it is Umbelliferae Bupleurum plant, identify that through the drug inspection office, Qinghai Province medical material is a Radix Bupleuri parvifolii, outward appearance is similar to Radix Bupleuri, its root pitchy has more sparse band and distinguishes with Radix Bupleuri;
(2) water of 10 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 2ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 459g.
3. the preparation method of a Radix Bupleuri extract is characterized in that, matrix preparation technology is as follows:
(1) stem and the leaf 3000g with Radix Bupleuri is dried and crushed into 20 orders, and 75% ethanol that adds 10 times of weight reflux, extract, 2 times in 75 ℃ was extracted 3 hours at every turn, and merge extractive liquid, filters, and reclaims ethanol and concentrates, and being concentrated into relative density is 1.0-1.1; Described Radix Bupleuri, the Latin name is called Bupleurum smithii Wolffvar.Pavuifolium Shan et Y.Li, it is Umbelliferae Bupleurum plant, identify that through the drug inspection office, Qinghai Province medical material is a Radix Bupleuri parvifolii, outward appearance is similar to Radix Bupleuri, its root pitchy has more sparse band and distinguishes with Radix Bupleuri;
(2) water of 8 times of volumes of adding in concentrated solution was placed 12 hours, filtered, and obtained filtrate;
(3) filtrate that step 2 is obtained is by D101 type macroporous resin column, and flow velocity is 4ml/min, and water elution reacts negative judgement water elution terminal point with Molishi; 70% ethanol elution is collected eluent, and TLC detects rutin and terminal point no longer occurs judging, reclaims ethanol, and 70 ℃ of vacuum dryings obtain Radix Bupleuri extract powder 735g.
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| CN102283910B (en) * | 2011-08-05 | 2013-04-17 | 浙江省中医药研究院 | Chinese medicinal composition with anti-depression effect and preparation and preparation method thereof |
| CN103340913A (en) * | 2013-07-18 | 2013-10-09 | 苏州市天灵中药饮片有限公司 | Method for extracting medicinal components of radix bupleuri |
| CN103479691B (en) * | 2013-09-30 | 2015-09-02 | 四川德培源中药科技开发有限公司 | Fat-reducing liver-protecting Chinese medicine composition |
| CN104523785A (en) * | 2014-05-28 | 2015-04-22 | 湖北荆山天然药业股份有限公司 | Extraction method of general flavone in honewort |
| CN105832785A (en) * | 2015-01-15 | 2016-08-10 | 邓学峰 | Pharmaceutical composition containing radix bupleuri extract and preparation thereof |
| CN106176851A (en) * | 2016-07-22 | 2016-12-07 | 南京正宽医药科技有限公司 | The process of preparing Chinese medicine extracting method of a kind of Radix Bupleuri decoction pieces and Radix Bupleuri compositions |
| CN110812376A (en) * | 2019-11-21 | 2020-02-21 | 宁夏职业技术学院 | Process for extracting total flavonoids from aerial parts of radix bupleuri cultivated in Ningxia |
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