CN101579059A - Feed additive for increasing milk cow dairy food quality and milk cow anti-heat stress - Google Patents
Feed additive for increasing milk cow dairy food quality and milk cow anti-heat stress Download PDFInfo
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Abstract
Feed additive for increasing milk cow dairy quality and milk cow anti-heat stress relates to a feed additive. The additive comprises calcium conjugated linoleic acid, selenium and vitamin E, the ratio of the additive is 15-50mg selenium of and 25,000-750,000 IU of vitamin E to each 1 Kg of calcium conjugated linoleic acid. The invention has the advantages that the yield of milk fat of milk cows, the contents of unsaturated fatty acid in milk fat and the selenium and VE in milk are increased, thus improving the dairy quality, the heat stress reaction of milk cows can also be alleviated, thus increasing the milk production , furthermore, the active oxygen in the heat stress milk cows can be reduced to decrease peroxidation reaction, thus reducing the injure of heat stress on milk cows and increasing the anti-oxidation ability of milk cow organism. The invention has the advantages of simple production process, no requirement of special equipment, safe and simple operation and no side effect.
Description
Technical field
The present invention relates to animal feed additive, be specifically related to a kind of feed addictive that is used to improve milk cow dairy food quality and anti heat stress for milk cow.
Background technology
CLA (is called for short CLA, below with) have anticancer, anti-oxidant, strengthen immunity, reduce fat deposition, promote growth and multiple beneficial such as angiocardiopathy preventing and diabetes in the biological function of human health, national research council (NRC) was classified CLA as unique a kind of animal sources aliphatic acid with antitumaous effect in 1996, the ruminant product is the main source of CLA in the human foods, wherein, the cla levels in the butterfat is the abundantest.Therefore, improve the content of CLA in the fat of milk cow, improve milk quality, significant to human health.Document " Kelly etc., J Nutr, 1998:881-885) " report is arranged, in dairy cow diet, directly add the content that vegetable fat or seed can improve CLA in the milk, can improve about 3 times usually.But when having reported again in the document " Zhou Qingan etc., Chinese oil, 2002:77-79 " if directly having joined grease in the dairy cow diet, feed feed intake of milk cow and cellulose digestion rate are descended.Its reason has: 1. grease is coated on cellulose surface, has limited cellulosic digestion; 2. grease is to the effect of cellulose-decomposing bacterium toxigenicity; 3. grease is applied the bag of microorganism species, has reduced the activity of microorganism surface enzyme; 4. the cation in LCFA and the cud forms insoluble compound, influences the needed ionic strength of microbial activities, or influences the pH value of cud because of the change of ion concentration.Therefore the content that how to improve CLA in the cow's milk of milk cow is a problem that is worth research.
Reported that at document " Giesy etc., J Dairy Sci, 2002:2023-2029 " directly adding calcium conjugated linoleic acid in feed can improve the relative amount of CLA in TFA in the butterfat, thereby can improve dairy food quality.But though the problem of existence is to have improved the relative amount of CLA in the butterfat, it can reduce butterfat percnetage simultaneously.Every ox is added calcium conjugated linoleic acid 50g or 100g every day, and butterfat percnetage descends 29% or 34%, thereby shows finally that the content of CLA remains unchanged or even decreases in the cow's milk.
In addition, milk cow is subjected to can producing physiological responses to heat stress when high temperature stimulates, promptly by acting on the caused general indication that comprises reaction of body nonspecific defense and specificity obstacle of HPA system.Heat stress can cause that the metabolism of milk cow gets muddled, production performance descends, milk production reduces, and milk composition changes, immunity weakens etc., and serious heat stress can cause the milk cow heatstroke, even dead.Heat stress also can cause the variation of anti-oxidant index in the cow serum, causes the unbalance of body antioxidant system.So the ability that how to improve anti heat stress for milk cow also is the problem that needs in the present raising dairy cattle solve.
Summary of the invention
The purpose of this invention is to provide a kind of milk cow milk-quality that both improved, the ability of anti heat stress for milk cow is improved, thereby improve the feed addictive of its output of milk.
The technical scheme that is adopted is to achieve the object of the present invention: provide a kind of improve milk cow dairy food quality and anti-heat stress feed addictive, it is made up of calcium conjugated linoleic acid, nanometer selenium and vitamin E, it consists of: per 1 kilogram of calcium conjugated linoleic acid adds nanometer selenium 15～50mg and vitamin E 25,000～75,000IU, every cow head adds the feed addictive of 100～400g every day.
Calcium conjugated linoleic acid is as a kind of fatty acid calcium, owing in cud, do not dissolve, only just dissolved in abomasum and small intestine, so the not influence of activity to microorganism in the cud can not influence lumen fermentation and feed digestibility, simultaneously fat is effectively utilized, energy can be provided, thereby the cow heat stress relief reaction improves production performance, reduces respiratory rate and rectal temperature etc.But calcium conjugated linoleic acid in vivo with external all easily oxidations, can cause that also butterfat suppresses.
Selenium is the requisite a kind of trace element of human body and animal; the lasting health of body is relevant with keeping; it is a kind of very strong antioxidant; poisonous peroxide can be reduced to innocuous substance; pair cell and tissue have protective effect; make it to avoid the infringement of peroxide, especially cell membrane and organelle film have special protective effect.Lack selenium, can cause important electrolyte sodium, potassium, the metabolism confusion of calcium and the increase of phat fat peroxide in the body.
VE has another name called tocopherol, adds the butterfat inhibition (Pottier etc., J.Dairy Sci.89:685-692) that VE can prevent that the high unsaturated fatty acid daily ration from causing in dairy cow diet.VE also is a kind of strong antioxidant, and VE and selenium have share synergy, and both can resist " free radical " infringement to body jointly, prevents that unrighted acid is oxidized, thereby keeps the balance of body antioxidant system.Though contain VE and selenium in the premix of the milk cow forage of routine, the content of its VE and selenium is lower, is not enough to bring about tangible results.The present invention is with a certain amount of calcium conjugated linoleic acid, nanometer selenium and the VE three's while feed addictive as milk cow, add to and feed milk cow in the feed, can alleviate milk cow is suppressed by the butterfat that calcium conjugated linoleic acid causes, the content that improves butterfat percnetage and fat yield and improve selenium, VE and CLA in the milk, the ability of anti heat stress for milk cow is improved, thereby improve its output of milk.
The additive provided by the present invention that every cow head adds 100～400g every day just can obviously improve milk quality, and can effectively alleviate the physiological responses to heat stress of milk cow and improve the antioxygenic property of milk cow.
It is that per 1 kilogram of calcium conjugated linoleic acid adds nanometer selenium 30mg and vitamin E 50 that the preferred feed addictive of the present invention is formed, 000IU, and every cow head adds the feed addictive of 200g every day.
The advantage of cow feed additive provided by the present invention is to improve the content of selenium, VE and CLA in the content of unrighted acid in fat of milk cow output and the butterfat and the milk; improve dairy food quality; can also effectively alleviate the physiological responses to heat stress of milk cow; improve the output of milk; can also reduce the active oxygen in the heat stress milk cow body in addition and reduce peroxidization; thereby reduce the damage of heat stress, improve the oxidation resistance of milk cow body milk cow.And production technology is simple, need not special equipment, and is safe and convenient to use, has no side effect.
Further set forth technical scheme of the present invention and beneficial effect below by embodiment, but be not limited to following embodiment.
The specific embodiment
Embodiment 1
Take by weighing the calcium conjugated linoleic acid (product of middle mountain eureka natural drug Co., Ltd of 100kg, contain c9t11 CLA-Ca about 50% and t10c12 CLA-Ca about 50%) and the nanometer selenium (product of Guangzhou Bo Shiao group) and 2 of 1.5g, 500, the vitamin E of 000IU (product of Guangzhou Bo Shiao group) mixes, and promptly gets feed addictive 1 of the present invention.
Embodiment 2
Take by weighing the nanometer selenium and 5,000 of calcium conjugated linoleic acid and the 3.0g of 100kg, the vitamin E of 000IU mixes, and promptly gets feed addictive 2 of the present invention (raw material sources are the same).
Embodiment 3
Take by weighing the nanometer selenium and 7,500 of calcium conjugated linoleic acid and the 4.5g of 100kg, the vitamin E of 000IU mixes, and promptly gets feed addictive 3 of the present invention (raw material sources are the same).
Embodiment 4 feed addictives are to the influence test of cow heat stress
4.1 experimental animal:
The multiparity or 30～45 days the output of milk of calving close (16-17kg/ head/sky), body weight are 28 of the healthy china holstein cowses of 500kg.
4.2 test specimen:
Sample: embodiment 1,2 and 3 feed addictive
4.3 test method:
28 holstein cows are divided into 4 groups (1 control group and 3 test group), 7 every group at random.The control group A basal diet of feeding, experiment group B basal diet+200g the additive 1 (from embodiment 1 obtain) of feeding, the test group C basal diet+200g additive 2 (from embodiment 2 obtain) of feeding, the test group D basal diet+200g additive 3 (from embodiment 3, obtaining) of feeding.The daily ration composition is prepared with reference to NRC (2001).Basal diet is made up of silage corn, alfalfa hay and concentrate supplement, and thick smart in being 49: 51, basal diet prescription and trophic level are as shown in Table 1 and Table 2.
Table 1 basal diet is formed
aThe composition of every kg premix: NaCl, 400g; NaHCO
3, 200g; I, 0.1g; Fe, 3g; Mg, 20g; Cu, 2g; Mn, 3g; Zn, 6g; Se, 0.06g; Co, 0.02g; VA, 800,000IU; VD, 100,000IU; VE, 4,000IU.
Table 2 basal diet nutrient composition content
The feeding and management of experimental animal: milk cow takes drylot feeding bolt system to raise, and TRM (TMR) is fed 2 times every day, freely drinks water, duct type machine milking every day 2 times (7:00,19:00).8 weeks of test duration, 1 week of preliminary trial period, regular 7 weeks of examination phase.About 2.0m place hangs wet-and-dry-bulb thermometer to duration of test apart from ground in cowshed, every day is (8:00,14:00 and 20:00 survey and average for three times) record cowshed temperature and relative humidity regularly, by formula: THI=0.80Td+ (Td-14.4) RH+46.4 calculates humidity-temperature index, wherein Td is a dry-bulb temperature, RH is a relative humidity, and THI is a humidity-temperature index.
The test determination index and the method for sampling: the output of milk, milk composition (butterfat, lactoprotein and lactose) content, butterfat aliphatic acid composition, respiratory rate, rectal temperature and the anti-oxidant index of serum are arranged at the main testing index of duration of test.The anti-oxidant index of serum comprises superoxide dismutase, glutathione peroxidase, MDA, active oxygen and TAC.
The mensuration of the output of milk, respiratory rate and rectal temperature: the output of milk, respiratory rate and the rectal temperature of measuring the single test milk cow in experimental period weekly.
The collection of milk sample and milk composition and aliphatic acid are measured: weekly the test milk cow is got the milk sample once in experimental period, the milk sample divides three parts; A part is used infrared ray milk constituents analysis-e/or determining milk composition immediately, and another part is used the content of vitamin E and selenium in high performance liquid chromatography and the atomic absorption spectroscopy determination milk respectively.Third part-20 ℃ freezing preservation waits and measures butterfat aliphatic acid with capillary gas chromatography-mass spectrometry method after the off-test and form.
The collection of blood sample and anti-oxidant index determining: after the off-test to test milk bovine jugular vein sterile blood sampling, leave standstill the centrifugal 15min of 2000r/min behind the 30min, the serum that drawing the upper strata does not have haemolysis is put in-20 ℃ of refrigerators and is preserved, and uses the anti-oxidant index of colorimetric method for determining after the off-test.
4.4 result of the test
The interior temperature of experimental period cowshed is 27.5～33.3 ℃, 30.1 ℃ of average out to.Humidity-temperature index (THI) is 74.1～83.7, average out to 78.8.It has been generally acknowledged that THI surpasses at 72 o'clock and can produce physiological responses to heat stress to milk cow, therefore, this duration of test milk cow is in the heat stress state.
As can be seen from Table 3, the output of milk of 3 test group (B, C and D) milk cow all is significantly higher than control group A, but there was no significant difference between each test group.The butterfat percnetage of experiment group B milk cow extremely significantly is lower than other each groups, but butterfat percnetage and the control group A of test group C and test group D milk cow do not have significant difference.The fat yield of test group C and test group D milk cow is significantly higher than test group A and experiment group B.Protein ratio and milk sugar rate do not have significant difference between control group and test group.
Table 3 additive is to the influence of milk production of cow and milk composition
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
As can be seen from Table 4, C4:0, C6:0, C12:0 and C16:0 content of fatty acid in 3 test group (B, C and D) fat of milk cow significantly are lower than control group A, C8:0, C10:0, C14:0 and C14:1 content of fatty acid extremely significantly are lower than control group A, C18:1, C18:2, CLA c9t11 and CLA t10c12 (unsaturated) the content of fatty acid utmost point are significantly higher than control group A, and C16:1, C18:0, C18:3 and C20:0 content of fatty acid and control group A do not have significant difference, but do not have significant difference between 3 test group.
The influence that table 4 additive is formed fat of milk cow aliphatic acid
Lowercase c represents cis in the aliphatic acid, and t represents trans, and CLA is a CLA.
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05)
Compare with control group A, selenium and the VE content of test group C and test group D milk cow significantly increase (table 5), but do not have significant difference between 3 test group (B, C and D).
Table 5 additive is to the influence of VE in the milk and Se content
Annotate: the different lowercase alphabet differentials of colleague's shoulder motes different significantly (P＜0.05).
Compare with control group A, the respiratory rate of 3 test group (B, C and D) milk cow extremely significantly descends, and rectal temperature significantly reduces, but does not have significant difference between 3 test group, the results are shown in Table 6.
Table 6 additive is to the influence of milk cow respiratory rate and rectal temperature
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
As can be seen from Table 7, additive can improve superoxide dismutase and the glutathione peroxidase activity of milk cow, and improves TAC.Compare with control group A, the superoxide dismutase of test group C and test group D and glutathione peroxidase are active significantly to raise, and TAC extremely significantly raises.Additive can also reduce the content of MDA and active oxygen, compares with control group A, and the MDA of test group C and test group D and active o content extremely significantly descend.Except that the glutathione peroxidase activity of test group D milk cow is significantly higher than the test group C, other anti-oxidant indexs do not have significant difference between two groups.
Table 7 additive is to the influence of the anti-oxidant index of cow serum
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Conclusion: feed addictive of the present invention can improve selenium and VE content in the content of CLA in fat of milk cow output and the butterfat and the milk, thereby improves milk-quality; Can alleviate simultaneously the physiological responses to heat stress of milk cow, output of milk increase, respiratory rate and rectal temperature are reduced; But also can improve the antioxygenic property of heat stress milk cow, and help keeping of the normal body condition of milk cow self body, reduce the generation of disease, also bring more economic benefit for the milk cow producer.Result of the test shows that effect is obvious for test group C and test group D additive therefor (additive 2 and additive 3), but difference on effect is little between two test group.Therefore determine that additive 2 is an optimum formula.
Embodiment 5 is the additive amount test under heat stress
5.1 experimental animal:
Multiparity or calving 30～45 days, 28 of the output of milk is close, body weight is close healthy china holstein cowses.
5.2 test specimen:
Sample: feed addictive 2 obtains from embodiment 2.
5.3 test method:
28 holstein cows are divided into 4 groups (1 control group and 3 test group), 7 every group at random.The control group A basal diet of feeding, the experiment group B basal diet+100g additive 2 of feeding, the test group C basal diet+200g additive 2 of feeding, the test group D basal diet+400g additive 2 of feeding.Daily ration is formed the preparation with reference to NRC (2001), and basal diet is formed and trophic level is seen embodiment 4.
The feeding and management of experimental animal: milk cow takes drylot feeding bolt system to raise, and TRM (TMR) is fed 2 times every day, freely drinks water, duct type machine milking every day 2 times (7:00,19:00).In 8 weeks of test duration, in 1 week of preliminary trial period, just trying 7 weeks of phase.The assay method of humiture is seen among the embodiment 4 described under 4.3 in the duration of test cowshed.
Test determination index: the output of milk, milk composition (butterfat, lactoprotein and lactose) content, butterfat aliphatic acid composition, milk cow respiratory rate and rectal temperature are arranged at the main testing index of duration of test.The assay method of each index is seen among the embodiment 4 described under 4.3.
5.4 result of the test
The interior temperature of experimental period cowshed is 24.3～33.1 ℃, 29.4 ℃ of average out to.Humidity-temperature index (THI) is 74.4～85.1, average out to 79.3.It has been generally acknowledged that THI surpasses 72 and can produce physiological responses to heat stress to milk cow, therefore, this duration of test milk cow is in the heat stress state.
As can be seen from Table 8, the additive that adds various dose can improve the output of milk of milk cow, when additive capacity reached 200g (test group C and D), the output of milk was significantly higher than control group A, but between test group C and the test group D and experiment group B and other respectively organize there was no significant difference.The additive of various dose is to the not obviously influence of milk composition of test milk cow.
Table 8 various dose additive is to the influence of milk production of cow and milk composition
Annotate: the different lowercase alphabet differentials of colleague's shoulder motes different significantly (P＜0.05).
As can be seen from Table 9, compare with control group A, C4:0, C6:0, C12:0 and C16:0 content of fatty acid in test group C and the test group D fat of milk cow significantly reduce; C8:0, C10:0, C14:0 and C14:1 content of fatty acid extremely significantly reduce; C18:1, C18:2, CLA c9t11 and CLA t10c12 content of fatty acid extremely significantly raise.But except that CLA c9t11 extremely significantly raise, other content of fatty acid differences were not obvious between test group C and the test group D.And C16:1, C18:0, C18:3 and C20:0 content of fatty acid do not have significant change.
Table 9 various dose additive is to the influence of fat of milk cow content of fatty acid
Annotate: lowercase c represents cis in the aliphatic acid, and t represents trans, and CLA is a CLA; The different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Interpolation various dose additive sees Table 10 to the influence of heat stress milk cow respiratory rate and rectal temperature.When additive capacity reached 200g (test group C and test group D), the respiratory rate of milk cow extremely significantly descended, and rectal temperature significantly reduces, but there was no significant difference between test group C and the test group D.
Table 10 various dose additive is to the influence of milk cow respiratory rate and rectal temperature
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Conclusion: effect is obvious when this additive addition reaches 200g, can improve the output of milk, but to not obviously influence of milk composition; Influence the composition of butterfat aliphatic acid simultaneously, in the reduction, the content of SCFA, increase the content of long-chain unsaturated fatty acid; Can also lower respiratory rate and the rectal temperature of heat stress milk cow, alleviate the physiological responses to heat stress of milk cow.But addition is 400g and addition is that difference on effect is little between the 200g.Determine with addition 200g to be good for saving cost.
Only add calcium conjugated linoleic acid in embodiment 6 milk cow forages and add additive contrast test of the present invention
6.1 experimental animal:
Multiparity or calving 30～45 days, 21 of the close healthy china holstein cowses of the output of milk and body weight.
6.2 test specimen:
Sample: calcium conjugated linoleic acid (middle mountain eureka natural drug Co., Ltd, contain c9t11 CLA-Ca about 50% and t10c12 CLA-Ca about 50%), feed addictive 2 (obtaining) from embodiment 2.
6.3 test method:
21 cow heads are divided into 3 groups at random, 7 every group.The control group A basal diet of feeding, the experiment group B basal diet+200g calcium conjugated linoleic acid of feeding, the test group C basal diet+200g additive 2 of feeding.Daily ration is formed the preparation with reference to NRC (2001), and basal diet is formed and trophic level is seen embodiment 4.
The feeding and management of experimental animal: milk cow takes drylot feeding bolt system to raise, and TRM (TMR) is fed 2 times every day, freely drinks water, duct type machine milking every day 2 times (7:00,19:00).In 8 weeks of test duration, in 1 week of preliminary trial period, just trying 7 weeks of phase.
Test determination index: the output of milk, milk composition (butterfat, lactoprotein and lactose) and the anti-oxidant index of serum are arranged at the main testing index of duration of test.The anti-oxidant index of serum comprises superoxide dismutase, glutathione peroxidase, MDA, active oxygen and TAC.The assay method of each index is seen among the embodiment 4 described under 4.3.
6.4 result of the test
As can be seen from Table 11, there was no significant difference between the output of milk of experiment group B milk cow and the control group A, but the output of milk of test group C milk cow is significantly higher than control group A.The milk butterfat percnetage of experiment group B extremely significantly is lower than control group A, and difference is not obvious between the milk butterfat percnetage of test group C and the control group A.Protein ratio and milk sugar rate do not have significant difference between control group and test group.
Table 11 calcium conjugated linoleic acid and additive are to the influence of milk production of cow and milk composition
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
As can be seen from Table 12, the anti-oxidant index of experiment group B milk cow is compared with control group A does not have significant difference, significantly raise but superoxide dismutase of test group C milk cow and glutathione peroxidase are active, MDA and active o content extremely significantly descend, and TAC extremely significantly raises.
Table 12 calcium conjugated linoleic acid and additive are to the influence of the anti-oxidant index of cow serum
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Conclusion: additive of the present invention can be alleviated the butterfat inhibition that calcium conjugated linoleic acid causes, improves butterfat percnetage, thereby improves the trophic function of milk; Additive can also improve the antioxygenic property of milk cow simultaneously, helps keeping of the normal body condition of milk cow self body, reduces the generation of disease.
The contrast experiment of embodiment 7 milk cows under heat stress and non-heat stress condition
7.1 experimental animal:
Respectively select 14 of multiparity or 30～45 days the output of milk of the calving healthy china holstein cowses close with body weight in summer and winter.
7.2 test specimen:
Sample: feed addictive 2 (from embodiment 2, obtaining).
7.3 test method:
With summer 14 holstein cows be divided into 2 groups (heat stress control group A and heat stress experiment group B), 7 every group at random; With winter 14 holstein cows be divided into 2 groups (non-heat stress control group C and non-heat stress test group D), 7 every group at random.Two control groups (A and C) basal diet of feeding, two test group (B and the D) basal diet+200g additive 2 (from embodiment 2 obtain) of feeding.The daily ration composition is prepared with reference to NRC (2001).Basal diet prescription and trophic level are shown in table 1 and table 2 among the embodiment 4.
The feeding and management of experimental animal: milk cow takes drylot feeding bolt system to raise, and TRM (TMR) is fed 2 times every day, freely drinks water, duct type machine milking every day 2 times (7:00,19:00).Summer, test and winter, test all continued for 4 weeks, in 1 week of preliminary trial period, was just trying 3 weeks of phase.The assay method of humiture is seen among the embodiment 4 described under 4.3 in the cowshed.
Test determination index: the output of milk, milk composition (butterfat, lactoprotein and lactose) content, butterfat aliphatic acid composition, milk cow respiratory rate, rectal temperature and the anti-oxidant index of serum are arranged at the main testing index of duration of test.The anti-oxidant index of serum comprises superoxide dismutase, glutathione peroxidase, MDA, active oxygen and TAC.The assay method of each index is seen among the embodiment 4 described under 4.3.
7.4 result of the test
The interior temperature of experimental period in summer cowshed is 25.3～32.9 ℃, 29.6 ℃ of average out to.Humidity-temperature index (THI) is 76.4～84.3, average out to 80.7.The interior temperature of experimental period in winter cowshed is 5.2～17.1 ℃, 13.6 ℃ of average out to.Humidity-temperature index (THI) is 30.4～64.3, average out to 57.8.It has been generally acknowledged that THI surpasses at 72 o'clock and can produce physiological responses to heat stress to milk cow, therefore, this test milk cow is in the heat stress state in summer, is in non-heat stress state in the winter time.
From table 13 and table 14 as can be seen, additive can significantly increase the output of milk of milk cow in heat stress phase and non-heat stress phase, but to the not obviously influence of butterfat percnetage, protein ratio and milk sugar rate.
Table 13 additive is the influence to milk production of cow and milk composition in the heat stress phase
Annotate: the different lowercase alphabet differentials of colleague's shoulder motes different significantly (P＜0.05).
Table 14 additive is the influence to milk production of cow and milk composition in the non-heat stress phase
Annotate: the different lowercase alphabet differentials of colleague's shoulder motes different significantly (P＜0.05).
From table 15 and table 16 as can be seen, additive can reduce the content of short chain and medium chain fatty acid (C4:0, C6:0, C8:0, C10:0, C12:0, C14:0, C14:1 and C16:0) in the fat of milk cow in heat stress phase and non-heat stress phase, but can increase the content of LCFA C18:1 and C18:2 in the butterfat.Simultaneously, c9t11CLA and t10c12CLA content all extremely significantly increased in heat stress phase and non-heat stress phase in the butterfat.
Table 15 additive is the influence to the fat of milk cow content of fatty acid in the heat stress phase
Lowercase c represents cis in the aliphatic acid, and t represents trans, and CLA is a CLA.
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05)
Table 16 additive is the influence to the fat of milk cow content of fatty acid in the non-heat stress phase
Lowercase c represents cis in the aliphatic acid, and t represents trans, and CLA is a CLA.
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05)
From table 17 and table 18 as can be seen, in heat stress period, additive can extremely significantly reduce the respiratory rate of milk cow and significantly reduce the rectal temperature of milk cow.But in non-heat stress period, additive has no significant effect respiratory rate and the rectal temperature of milk cow.
Table 17 additive is the influence to milk cow respiratory rate and rectal temperature in the heat stress phase
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Table 18 additive is the influence to milk cow respiratory rate and rectal temperature in the non-heat stress phase
From table 19 and table 20 as can be seen, additive is in can significantly the raise activity of superoxide dismutase and glutathione peroxidase of heat stress phase and non-heat stress phase; Extremely significantly reduce the content of MDA and active oxygen, and significantly improve TAC.
Table 19 additive is answered the influence of phase to the anti-oxidant index of cow serum in heat
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Table 20 additive is answered the influence of phase to the anti-oxidant index of cow serum in non-heat
Annotate: the different capitalizations of colleague's shoulder motes are represented difference extremely significantly (P＜0.01), different lowercase alphabet differentials different significantly (P＜0.05).
Conclusion: this additive can increase the content of CLA in milk production of cow and the butterfat in heat stress phase and non-heat stress phase, thereby improves milk-quality, but to not obviously influence of milk composition.Additive can reduce respiratory rate and the rectal temperature of milk cow period at heat stress, helps alleviating the physiological responses to heat stress of milk cow.But respiratory rate and rectal temperature in the non-heat stress phase to milk cow have no significant effect.In heat stress phase and non-heat stress phase, additive can improve the antioxygenic property of milk cow, helps keeping of the normal body condition of milk cow self body, reduces the generation of disease.
Claims (2)
1, a kind of feed addictive that improves milk cow milk-quality and anti-heat stress, it is characterized in that: form by calcium conjugated linoleic acid, nanometer selenium and vitamin E, it consists of: per 1 kilogram of calcium conjugated linoleic acid adds nanometer selenium 15～50mg and vitamin E 25,000～75,000IU, every cow head adds the feed addictive of 100～400g every day.
2, the feed addictive of raising milk cow milk-quality according to claim 1 and anti-heat stress is characterized in that: per 1 kilogram of calcium conjugated linoleic acid adds nanometer selenium 30mg and vitamin E 50,000IU, and every cow head adds the feed addictive of 200g every day.
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| CNA200910040632XA CN101579059A (en) | 2009-06-29 | 2009-06-29 | Feed additive for increasing milk cow dairy food quality and milk cow anti-heat stress |
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| CNA200910040632XA CN101579059A (en) | 2009-06-29 | 2009-06-29 | Feed additive for increasing milk cow dairy food quality and milk cow anti-heat stress |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101965926A (en) * | 2010-10-20 | 2011-02-09 | 西南大学 | Daily ration for milking cow producing high-conjugated linoleic acid (CLA) milk |
| CN102406064A (en) * | 2010-09-26 | 2012-04-11 | 山东六和集团有限公司 | Feed for meat duck high in carcass quality |
| CN102550849A (en) * | 2010-12-14 | 2012-07-11 | 内蒙古伊利实业集团股份有限公司 | Functional milk cow feed additive capable of resisting heat stress and improving milk quality |
| CN101731481B (en) * | 2010-02-01 | 2012-09-26 | 河北农业大学 | Milk cow premix compound and preparation method thereof |
| CN103027197A (en) * | 2013-01-06 | 2013-04-10 | 杭州世博生物技术开发研究所有限公司 | Mineral feed additive containing nano-selenium and preparation method and applications thereof |
| CN104431362A (en) * | 2014-12-08 | 2015-03-25 | 湖南泰丰动物药业有限公司 | Feed additive capable of improving oxidative stress of dairy cows and application method of feed additive |
| CN107549484A (en) * | 2017-09-28 | 2018-01-09 | 淮北市渭水源农业有限公司 | A kind of feed addictive of anti heat stress for milk cow and preparation method thereof |
| CN107743923A (en) * | 2017-11-18 | 2018-03-02 | 堵美娟 | A kind of cultural method for reducing ox heat stress oxidative damage |
| CN112868917A (en) * | 2021-01-23 | 2021-06-01 | 北京市畜牧总站 | Selenium-rich feed composition for dairy cows and application thereof |
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2009
- 2009-06-29 CN CNA200910040632XA patent/CN101579059A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731481B (en) * | 2010-02-01 | 2012-09-26 | 河北农业大学 | Milk cow premix compound and preparation method thereof |
| CN102406064A (en) * | 2010-09-26 | 2012-04-11 | 山东六和集团有限公司 | Feed for meat duck high in carcass quality |
| CN101965926A (en) * | 2010-10-20 | 2011-02-09 | 西南大学 | Daily ration for milking cow producing high-conjugated linoleic acid (CLA) milk |
| CN102550849A (en) * | 2010-12-14 | 2012-07-11 | 内蒙古伊利实业集团股份有限公司 | Functional milk cow feed additive capable of resisting heat stress and improving milk quality |
| CN102550849B (en) * | 2010-12-14 | 2013-11-13 | 内蒙古伊利实业集团股份有限公司 | Functional milk cow feed additive capable of resisting heat stress and improving milk quality |
| CN103027197A (en) * | 2013-01-06 | 2013-04-10 | 杭州世博生物技术开发研究所有限公司 | Mineral feed additive containing nano-selenium and preparation method and applications thereof |
| CN104431362A (en) * | 2014-12-08 | 2015-03-25 | 湖南泰丰动物药业有限公司 | Feed additive capable of improving oxidative stress of dairy cows and application method of feed additive |
| CN107549484A (en) * | 2017-09-28 | 2018-01-09 | 淮北市渭水源农业有限公司 | A kind of feed addictive of anti heat stress for milk cow and preparation method thereof |
| CN107743923A (en) * | 2017-11-18 | 2018-03-02 | 堵美娟 | A kind of cultural method for reducing ox heat stress oxidative damage |
| CN112868917A (en) * | 2021-01-23 | 2021-06-01 | 北京市畜牧总站 | Selenium-rich feed composition for dairy cows and application thereof |
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Open date: 20091118 |