CN101575807A - Method for preparing lining material by organism colliquefaction - Google Patents
Method for preparing lining material by organism colliquefaction Download PDFInfo
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- CN101575807A CN101575807A CNA2009101118438A CN200910111843A CN101575807A CN 101575807 A CN101575807 A CN 101575807A CN A2009101118438 A CNA2009101118438 A CN A2009101118438A CN 200910111843 A CN200910111843 A CN 200910111843A CN 101575807 A CN101575807 A CN 101575807A
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- pseudomonad
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- shoe lining
- suspension liquid
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Abstract
The invention discloses a method for preparing lining material by organism colliquefaction, including the following steps: (a) organism pseudomonad suspending liquid up to the standards is put into a plastic can; (b) lining textile material is put into the plastic can to be soaked for 48 hours in the ratio that 1kg organism pseudomonad suspending liquid soaks 1-2 meters of the lining textile material when the water temperature of the organism pseudomonad suspending liquid is 20-28 DEG C and the room temperature is 25 DEG C, so as to lead the organism bacteria suspending liquid to form even and stable colony substance system; (c) the soaked penetrative lining textile material is naturally dried for 24 hours at room temperature of 15-30 DEG C, is roasted in an oven at 40 DEG C for 12 hours, and is made into the lining material containing organism. The lining material of the invention contains substances which can degrade pathogenic bacteria and decompose odor substance hidden in shoe cavity, thus achieving antibacterial and deodorant effect.
Description
Technical field
The present invention relates to a kind of method of preparing lining material by organism colliquefaction.
Background technology
Pin is one of the most flourishing position of human body distal end sweat gland, also is the abundantest position of nerve ending simultaneously, and the position of motion, frictional force maximum.When pin sweat generates heat under the motion and the effect of frictional force, the pore diastole begins perspire.The skin of pin can be breathed, and constantly discharges carbon dioxide gas, and along with the increase of ambient temperature, the discharge capacity of carbon dioxide gas also increases, and pin has the sensation of feeling suffocated, and the sweat that goes out of the high more pin of temperature is just many more like this.Beriberi (pin is smelly) is because the eccrine secretion of health is vigorous, the sweat gland secretion thing bacterium, mould divide take off produce dirty smelly.Perspiration impels bacterium to breed easily.Traditional shoe lining material is made by some cotton kind fabrics usually, has absorbing sweat and ventilative effect.But because service time is long, the perspiration of pin still can cause a large amount of breedings of bacterium and produce stink, particularly for the vigorous people of some sweat secretions, soon, suffers from diseases such as beriberi easily because bacterial reproduction is many.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide the manufacture method of the shoe lining material of a kind of fused organism, organism among the present invention is by absorption, decomposition and transformation, prevent the generation of harmful substance ammonia and amine, degrade germ in some potential footwear chamber, make concentration and the toxicity reduction or the disappearance of the pollutant in the footwear chamber, decompose the material that causes stink, reach the effect of antibacterial and deodouring, antiviral, the anti-infective factor that organism contains, can activate the immune system of body, the stress reaction process of strengthening body can suppress pin sweat and keep foot salubrious.
For reaching above-mentioned purpose, the present invention adopts following technical scheme:
The manufacture method of the shoe lining material of a kind of fused organism may further comprise the steps: (a) select the biological pseudomonad suspension liquid that is up to the standards for use, leave in the plastic tank; (b) 25 ℃ of room temperatures, during water temperature that biological pseudomonad suspension liquid is 20 ℃～28 ℃, ratio in the biological pseudomonad suspension liquid immersion of 1kg 1-2 rice shoe lining textile material, the shoe lining textile material is put into plastic tank to be soaked, soak time is 48 hours, makes the biological bacteria suspension liquid form uniform and stable bacterium colony substance system; (c) the shoe lining textile material that will soak infiltration enters 40 ℃ interior the baking 12 hours of baking oven after drying 24 hours naturally under 15 ℃～30 ℃ room temperature conditions, manufactures the shoe lining material of fused organism.When the shoe lining textile material is immersed in the biological pseudomonad suspension liquid, be full of living things system between the fiber of fabric, in biological bacteria suspension liquid volatilization process, biological substance is attached on the fiber of fabric with the fungus strain form, and firmly and not come off, between fabric, form complete biological chain.With the temperature of pin, sweat and the pin discharge carbon dioxide gas that pin goes out is the energy, relies on the effect of organism enzyme to transmit decomposition, to obtain the carrying out required energy of vital movement, so just can keep the bactericidal action of long period.The water content of the shoe lining material of the fused organism of making wants≤5%; Organism is attached to effective viable bacteria content 〉=1,000,000,000 on the material/gram.
The ratio that described biological pseudomonad suspension liquid soaks the shoe lining textile material is the ratio that the biological pseudomonad suspension liquid of 1kg soaks 2 meters shoe lining textile materials.
The preparation method of described biological pseudomonad suspension liquid is: biological pseudomonad original bacteria liquid contains viable bacteria amount and is not less than 3,000,000,000/ml, with the raw material of the culture medium ratio by 1: 100, after the thin up stirring, 26 ℃～30 ℃ of water temperatures; Between the pH in 7.0 to 8.0, leave standstill 5 days after, cultivate and to make biological pseudomonad suspension liquid.
Described pseudomonad is many thalline mixed liquor, comprises red pseudomonads R hodopseudomonas, red spirillum Rhodospirillum, Candida pulcherrima Candidapulcherrima, shadow yeast Spororbolomyces roseus, Rhodotorula RhodotorulaHarrisom.
Biological pseudomonad kind is a kind ofly to make the energy and make carbon source with carbon dioxide or small organic molecule with light, makes hydrogen donor with hydrogen sulfide etc., carries out fully from nourishing one's nature or photoheterotrophy growth but a quasi-microorganism of non-oxygen-production.
The diversity of biological pseudomonad kind physiology type makes it become one of flora the most complicated in the bacterium.Under different natural environments, it can show different biochemical functions, as fixed nitrogen, solid carbon, dehydrogenation, sulfide oxidation etc.Biological pseudomonad kind mechanism is a kind of anaerobic processes basically; Owing to there is not the photochemical reaction system, so the photosynthesis process is not made hydrogen donor with water, the photodissociation of water does not take place, do not discharge molecular oxygen yet; The hydrogen donor of reduction CO2 is sulfide, molecular hydrogen or organic matter.
Pseudomonad is because the diversity of its physiology monoid, the uniqueness of carbon, nitrogen metabolism approach and photosynthesis mechanism and be subjected to people's attention.Pseudomonad contains antibacterium, antiviral material, the pathogenicity and the inhibition pathogenic growth of these materials energy passivation pathogen.The activity of photosynthetic bacteria simultaneously can promote beneficial microbe propagation such as actinomyces, suppresses pernicious bacteria growths such as filamentous fungi, thereby suppresses some generation of planting disease effectively and spread.
The test stone of the biological pseudomonad suspension liquid that is up to the standards is:
The monad culture medium: sodium dihydrogen phosphate, malic acid, sodium acetate, NaOH, ammonium chloride, magnesium chloride, calcium chloride are molten, distilled water, growth factor solution and trace element solution, measure between the pH in 6.5 to 7.0.
The colonial morphology check: degerming in gnotobasis, is filled the plate plate, thick about 3-5mm after handling.Get photosynthetic bacteria liquid after solidifying and do plate streaking, the plate anaerobism is cultivated illumination 3-5 days, 30 ℃ of room temperatures grew red bacterium colony after 3-5 days, and observing colonial morphology is straw hat shape or garden shape, smooth surface, and few protuberance, neat in edge takes on a red color.
Get one of original bacteria liquid and put (lid cover plate) (16 * 40) observation bacterium form size and motion state under high power lens on the slide glass.
One of bacterium liquid in the cultivation is made smear, air dry, alcolhol burner flame is fixed, through just dying (crystal violet), mordant dyeing (Lu Shi iodine liquid), decolouring (95% ethanol), redye (sarranine dye liquor) after, oily mirror (16 * 100) is observed, thalline takes on a red color.
Subjective inspection: measure the original bacteria liquid 500ml after diluting 100 times, put in the triangular flask, detect by an unaided eye, fermented flavour is arranged to the red suspension body.The product that leaves standstill existing precipitation for a long time should vibrate or stir 10-20 sampling again after second earlier.
Total bacteria count measuring: get 0.5ml after will fully vibrating through the bacterium liquid after handling in the subjective inspection step and be diluted to 200ml with aqua sterilisa, the microlitre of therefrom peeking after stirring evenly fills plate and goes into (every big lattice 0.1mm3 in the counting chamber of blood counting chamber, put under 600 power microscopes and observe, simultaneously with red-cell count method note bacterium number, be every milliliter of total bacteria count with every lattice bacterium number average on duty 100,000,000 of 25 little lattice.Total plate count: be not less than 3,000,000,000/ml, viable count is not less than 2,000,000,000/ml;
Viable count is measured: the bacterium liquid after will handling in the total bacteria count measuring step dilutes 100000 times again, getting 1ml is added in the 10ml monad culture medium, mix the back and add 20g/1000ml concentration agar solution 10ml, solidify in the horizontalization ware, add glass cover 25-30 ℃ of temperature, under the gnotobasis, 60 watts of incandescent lamp irradiations, apart from bulb 12cm, observe the counting clump count after 1-2 days, calculate with following formula: the viable count=colony counting in every ml sample/25 * 100,000,000.
Described shoe lining textile material adopts a kind of in fine canvas, cotton dimension blended cloth, polyvinyl cloth, Li Xinbu, plain, Fu Rongbu or the plaids.
The invention has the beneficial effects as follows: the present invention is the biotechnology new breakthrough, the basic element of organism will be cultivated into, constantly carrying out from the inorganic environment to the biocoene, cyclic process from the biocoene to the inorganic environment again, the multiple flora of organism is fused in the shoe lining textile material, biocoene and inorganic environment effect constitute biological chain, interregional at regular hour and shoe lining textile material, circulate and mobile formed organic unity body, i.e. the natural biology catenary system of shoe lining textile material of interacting of energy by material between the multiple organism and between the inorganic environment.Fuse organism physiology type diversity, under different natural environments, can show different biochemical functions, can breathe, the variation of the condition that can conform and change the mode that it obtains energy in the shoe lining textile material.Fusing in important microbe-derived of the organism of shoe lining textile material is natural colouring matter, and this pigment is nontoxic, and is bright in luster, glossy, and the tool water proofing property.Organism is by absorption, decomposition and transformation, prevent the generation of harmful substance ammonia and amine, degrade germ in some potential footwear chamber, make the concentration of the pollutant in the footwear chamber and the process that toxicity reduces or disappears, decompose the material that causes stink, reach the effect of antibacterial and deodouring, antiviral, the anti-infective factor that organism contains, can activate the immune system of body, the stress reaction process of strengthening body can suppress pin sweat and keep foot salubrious.
The specific embodiment
Embodiment 1
The manufacture method of the shoe lining material of a kind of fused organism of present embodiment, may further comprise the steps: (a) prepare biological pseudomonad suspension liquid earlier, employing comprises red pseudomonads R hodopseudomonas, red spirillum Rhodospirillum, Candida pulcherrima Candidapulcherrima, shadow yeast Spororbolomyces roseus, the biological pseudomonad original bacteria liquid of Rhodotorula RhodotorulaHarrisom, contain the viable bacteria amount and be not less than 3,000,000,000/ml, use the monad culture medium, raw material was in 1: 100 ratio, after thin up stirs, 26 ℃～30 ℃ of water temperatures; Between the pH in 7.0 to 8.0, leave standstill 5 days after, cultivate and to make biological pseudomonad suspension liquid.Getting the biological pseudomonad suspension liquid of 1kg leaves in the plastic tank of 2kg capacity; (b) the shoe lining textile material adopts a kind of in fine canvas, cotton dimension blended cloth, polyvinyl cloth, Li Xinbu, plain, Fu Rongbu or the plaids.25 ℃ of room temperatures, during water temperature that biological pseudomonad suspension liquid is 20 ℃～28 ℃, ratio in 2 meters shoe lining textile materials of the biological pseudomonad suspension liquid immersion of 1kg, 2 meters shoe lining textile materials are put into plastic tank to be soaked, soak time is 48 hours, makes the biological bacteria suspension liquid form uniform and stable bacterium colony substance system; (c) the shoe lining textile material that will soak infiltration enters 40 ℃ interior the baking 12 hours of baking oven after drying 24 hours naturally under 15 ℃～30 ℃ room temperature conditions, manufactures the shoe lining material of fused organism.When the shoe lining textile material is immersed in the biological pseudomonad suspension liquid, be full of living things system between the fiber of fabric, in biological bacteria suspension liquid volatilization process, biological substance is attached on the fiber of fabric with the fungus strain form, and firmly and not come off, between fabric, form complete biological chain.With the temperature of pin, sweat and the pin discharge carbon dioxide gas that pin goes out is the energy, relies on the effect of organism enzyme to transmit decomposition, to obtain the carrying out required energy of vital movement, so just can keep the bactericidal action of long period.Water content≤5% of the shoe lining material of the fused organism of making; Organism is attached to effective viable bacteria content 〉=1,000,000,000 on the material/gram.
Embodiment 2
The manufacture method of the shoe lining material of a kind of fused organism of present embodiment, may further comprise the steps: (a) prepare biological pseudomonad suspension liquid earlier, employing comprises red pseudomonads R hodopseudomonas, red spirillum Rhodospirillum, Candida pulcherrima Candidapulcherrima, shadow yeast Spororbolomyces roseus, the biological pseudomonad original bacteria liquid of Rhodotorula RhodotorulaHarrisom, contain the viable bacteria amount and be not less than 3,000,000,000/ml, use the monad culture medium, raw material was in 1: 100 ratio, after thin up stirs, 26 ℃～30 ℃ of water temperatures; Between the pH in 7.0 to 8.0, leave standstill 5 days after, cultivate and to make biological pseudomonad suspension liquid.Getting the biological pseudomonad suspension liquid of 1kg leaves in the plastic tank of 2kg capacity; (b) the shoe lining textile material adopts a kind of in fine canvas, cotton dimension blended cloth, polyvinyl cloth, Li Xinbu, plain, Fu Rongbu or the plaids.25 ℃ of room temperatures, during water temperature that biological pseudomonad suspension liquid is 20 ℃～28 ℃, ratio in 1 meter shoe lining textile material of the biological pseudomonad suspension liquid immersion of 1kg, 1 meter shoe lining textile material is put into plastic tank to be soaked, soak time is 48 hours, makes the biological bacteria suspension liquid form uniform and stable bacterium colony substance system; (c) the shoe lining textile material that will soak infiltration enters 40 ℃ interior the baking 12 hours of baking oven after drying 24 hours naturally under 15 ℃～30 ℃ room temperature conditions, manufactures the shoe lining material of fused organism.When the shoe lining textile material is immersed in the biological pseudomonad suspension liquid, be full of living things system between the fiber of fabric, in biological bacteria suspension liquid volatilization process, biological substance is attached on the fiber of fabric with the fungus strain form, and firmly and not come off, between fabric, form complete biological chain.With the temperature of pin, sweat and the pin discharge carbon dioxide gas that pin goes out is the energy, relies on the effect of organism enzyme to transmit decomposition, to obtain the carrying out required energy of vital movement, so just can keep the bactericidal action of long period.Water content≤5% of the shoe lining material of the fused organism of making; Organism is attached to effective viable bacteria content 〉=1,000,000,000 on the material/gram.
Claims (5)
1, the manufacture method of the shoe lining material of a kind of fused organism is characterized in that may further comprise the steps: (a) select the biological pseudomonad suspension liquid that is up to the standards for use, leave in the plastic tank; (b) 25 ℃ of room temperatures, during water temperature that biological pseudomonad suspension liquid is 20 ℃～28 ℃, ratio in the biological pseudomonad suspension liquid immersion of 1kg 1-2 rice shoe lining textile material, the shoe lining textile material is put into plastic tank to be soaked, soak time is 48 hours, makes the biological bacteria suspension liquid form uniform and stable bacterium colony substance system; (c) the shoe lining textile material that will soak infiltration enters 40 ℃ interior the baking 12 hours of baking oven after drying 24 hours naturally under 15 ℃～30 ℃ room temperature conditions, manufactures the shoe lining material of fused organism.
2, the manufacture method of the shoe lining material of fused organism as claimed in claim 1, it is characterized in that: the ratio that described biological pseudomonad suspension liquid soaks the shoe lining textile material is the ratio that the biological pseudomonad suspension liquid of 1kg soaks 2 meters shoe lining textile materials.
3, the manufacture method of the shoe lining material of fused organism as claimed in claim 1, it is characterized in that: the preparation method of described biological pseudomonad suspension liquid is: biological pseudomonad original bacteria liquid, contain the viable bacteria amount and be not less than 3,000,000,000/ml, with the raw material of monad culture medium ratio in 1: 100, after thin up stirs, 26 ℃～30 ℃ of water temperatures; Between the pH in 7.0 to 8.0, leave standstill 5 days after, cultivate and to make biological pseudomonad suspension liquid.
4, the manufacture method of the shoe lining material of fused organism as claimed in claim 3, it is characterized in that: described pseudomonad is many thalline mixed liquor, includes red pseudomonad, red spirillum, Candida pulcherrima, shadow yeast, Rhodotorula.
5, the manufacture method of the shoe lining material of fused organism as claimed in claim 1 is characterized in that: described shoe lining textile material adopts a kind of in fine canvas, cotton dimension blended cloth, polyvinyl cloth, Li Xinbu, plain, Fu Rongbu or the plaids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101118438A CN101575807B (en) | 2009-05-25 | 2009-05-25 | Method for preparing lining material by organism colliquefaction |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009101118438A CN101575807B (en) | 2009-05-25 | 2009-05-25 | Method for preparing lining material by organism colliquefaction |
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| CN101575807A true CN101575807A (en) | 2009-11-11 |
| CN101575807B CN101575807B (en) | 2011-03-30 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103637463A (en) * | 2013-11-15 | 2014-03-19 | 青岛亨达股份有限公司 | Manufacturing method for shoe with lining made of blood bacterium resisting material |
| US9193940B2 (en) | 2006-08-11 | 2015-11-24 | Novozymes Biologicals, Inc. | Bacteria cultures and compositions comprising bacteria cultures |
| US9982382B2 (en) | 2011-02-15 | 2018-05-29 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
| CN109837762A (en) * | 2019-01-16 | 2019-06-04 | 泉州华光职业学院 | A kind of preparation method of antibacterial and deodouring textile material |
-
2009
- 2009-05-25 CN CN2009101118438A patent/CN101575807B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9193940B2 (en) | 2006-08-11 | 2015-11-24 | Novozymes Biologicals, Inc. | Bacteria cultures and compositions comprising bacteria cultures |
| US9982382B2 (en) | 2011-02-15 | 2018-05-29 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
| US10577738B2 (en) | 2011-02-15 | 2020-03-03 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
| US10968556B2 (en) | 2011-02-15 | 2021-04-06 | Novozymes Biologicals, Inc. | Mitigation of odor in cleaning machines and cleaning processes |
| CN103637463A (en) * | 2013-11-15 | 2014-03-19 | 青岛亨达股份有限公司 | Manufacturing method for shoe with lining made of blood bacterium resisting material |
| CN109837762A (en) * | 2019-01-16 | 2019-06-04 | 泉州华光职业学院 | A kind of preparation method of antibacterial and deodouring textile material |
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| Publication number | Publication date |
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| CN101575807B (en) | 2011-03-30 |
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Effective date of registration: 20110617 Address after: 362211 Fujian city of Jinjiang province Chendai Andou Industrial Zone No. 143 Patentee after: Jinjiang Jinwei Sports Products Co.,Ltd. Address before: 2, 350000, Yonghe garden, No. 410 Pu Pu Avenue, Cangshan District, Fujian, Fuzhou, 405 Patentee before: Lin Shuqi |
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Granted publication date: 20110330 Termination date: 20130525 |