CN101570725A - Method for starter propagation by utilizing smoked vinegar lees - Google Patents

Method for starter propagation by utilizing smoked vinegar lees Download PDF

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Publication number
CN101570725A
CN101570725A CNA2009101419401A CN200910141940A CN101570725A CN 101570725 A CN101570725 A CN 101570725A CN A2009101419401 A CNA2009101419401 A CN A2009101419401A CN 200910141940 A CN200910141940 A CN 200910141940A CN 101570725 A CN101570725 A CN 101570725A
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vinegar
smoked
smoked vinegar
poor
fermented
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CN101570725B (en
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宋春雪
张茜
李丽华
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Shanxi Jinlongyu Liangfen vinegar industry Co.,Ltd.
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SHANXI SUNSHINE INDUSTRIAL DEVELOPMENT Co Ltd
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Abstract

The invention relates to the food processing field, in particular to a method for starter propagation by utilizing smoked vinegar lees, which solves the problems that the quality of source of the starter propagation raw material is unstable, the quality of the thickness of bran is difficult to control, and the leftovers of the starter propagation industry-the smoked vinegar lees have large output and pollutes the environment. The method comprises the following steps: (1) firstly, black-koji mould stock seed is made into seed culture; (2) fresh smoked vinegar lees and nutrient fluid are mixed to form a mixing material; water is added into the mixing material for the adjusting the moisture of the mixing material to be 60-65%; the nutrient fluid comprises corn dextrin, glucose, magnesium sulfate and the like; (3) the black-koji mould stock seed is reinoculated; (4) the reinoculated material is fermented; (5) the fermented bran koji is laid to be thin and dried. The obtained bran-koji enzyme activity is increased by more than 30%; the fermented time is shortened by 6 to 9 hours; the skin of the fermented started is thin, and the distribution surface of mycelial is wide and uniform and has rich start smell. The method not only can solve the pollution of the vinegar lees to the environment, can save the production cost for vinegar making industries and has good economic and social benefits.

Description

A kind of method of utilizing the smoked vinegar lees song
Technical field
The present invention relates to food processing field, be specially a kind of method of utilizing the smoked vinegar lees song.
Background technology
At present China's koji industry adopts be with wheat bran as major ingredient, remove thin part after wheat bran need being sieved usually, utilize the bran koji, mainly utilize its area big, the characteristics of good permeability.But because wheat bran is the by product of milling plant, raw material sources instability, and the thickness poor quality of wheat bran control are unfavorable for the growth of aerobic bacterial classification, need to sieve once more processing simultaneously, and production cost is higher.
China particularly Shanxi vinegar manufacturing enterprise is numerous, product category is more, correspondingly production technique is various, wherein smoked vinegar is with the high-quality jowar, barley, pea is made raw material, adopts Daqu, fast bent diastatic fermentation prolongs zymamsis, the inoculation of acetify high temperature is ignited, the smoked vinegar unstrained spirits that drenches is in conjunction with waiting special process, through steaming, ferment, smoked, drench, shine five steps, wherein smoking technique is the most key, the vinegar unstrained spirits that makes is inserted in the smoked cylinder, add a cover, smoke with fire the outside, from the raw material behind the vinegar unstrained spirits being put into the yellow of smoked cylinder, to smoking into chocolate, once darken once through six days six times inverted engine, smoking technique has increased color for the Shanxi vinegar, increased the fragrance of grain, promoted esterification process, color and luster is reddish brown shinny, and concentration is suitable, heavy flavour of vinegar fragrance, ester perfume (or spice) is pure, and continuous acid is tasty and refreshing, and contains multiple organic acid, amino acid, reducing sugar, alcohols, ester class etc.Remaining tankage are referred to as " smoked vinegar is poor " after adopting above-mentioned technology to make vinegar, on the one hand, a large amount of raw materials is utilized in the process of fermentation, on the other hand, also have some remaining nutritive substances not to be utilized, the basic substance that contains microorganism growth is as remaining starch, several mineral materials, amino acid and organic acid etc.
In system vinegar industry, the poor amount of the smoked vinegar that goes out every day is very big, but smoked vinegar is poor to be acid organism, dissolves naturally slowly, can directly produce serious pollution to environment, and it has characteristics such as calorific value is low, water capacity is big, content of cellulose height, having determined that the poor processing of smoked vinegar is quite difficult, is a great problem of system vinegar industry, up to the present, not seeing has any smoked vinegar is pickled with grains or in wine to carry out the effective ways of waste reclaimation, and also not seeing has the report that utilizes the smoked vinegar lees song.
Summary of the invention
The present invention comes the source quality instability in order to solve existing starter-making materials, thickness poor quality's control of wheat bran, problems such as processing once more, cost is higher and the poor output of the smoked vinegar of tankage of system vinegar industry is big, difficult, contaminate environment provide a kind of method of utilizing the smoked vinegar lees song.
The present invention adopts following technical scheme to realize: a kind of method of utilizing the smoked vinegar lees song, may further comprise the steps: (1) at first makes kind of a bacterium with the black-koji mould original seed, the method that described black-koji mould is made kind of bacterium by original seed is the known technology of this area, for example can expand numerous, the solid-state cultivation of secondary by the one-level test tube; (2) poor and add-on is that 2~10% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 60~65%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 1~2%, glucose 0.2~1%, sal epsom 0.02~0.08%, ammonium nitrate 1.0~2.0%, Repone K 0.2~0.6%, ferrous sulfate 0.005~0.01%, bean powder 0.5~1.5%, all the other are water, and described nutritive medium is rich in carbon, nitrogenous source and various trace elements, VITAMIN, with supply smoked vinegar poor in the deficiency of various nutritive ingredients, help the growth of black-koji mould, blastoprolepsis, mycelia is strong, enzyme is lived improve greatly, this nutrient solution prescription and proportioning are that the applicant is according to the poor nutritive ingredient of smoked vinegar, come through test of many times and summary of experience, the smoked vinegar that adds nutritive medium is poor, and it is best that its nutritive ingredient and content reach; (3) press the good aspergillus niger strain bacterium of 3~5% inoculation culture of mixture weight; (4) postvaccinal material is put into the ventilating fermentation pond and fermented about 40~50 hours, the temperature of fermentation is 30~35 ℃; (5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.
Through raw material being smoked the poor check of unstrained spirits, wherein nutrient composition content (referring to the over dry amount of substance) mean value: coarse ash 12.96%, crude fat 7.49%, crude protein 15.94%, robust fibre 30.5%; According to the moisture of 60-65%, its content is: coarse ash 5-8%, crude fat 3-5%, crude protein 7-11%, robust fibre 17-20%.
For further proof the method applied in the present invention can reach good effect, carried out following test, promptly utilize smoked vinegar poor production wheat bran and tradition to utilize wheat bran to produce wheat bran and compare experiment, specifically see following laboratory report:
One, test objective: utilize the poor contrast experiment who produces wheat bran of wheat bran and smoked vinegar
Two, test materials and equipment:
A, bacterial classification: aspergillus niger (uv-11)
B, substratum:
Bran mass: wheat bran, water; The poor substratum of smoked vinegar: smoked vinegar is poor, water, nutritive medium
C, testing installation: ventilating fermentation pond
Three, experimental procedure:
The activation of A, bacterial classification: the preservation aspergillus niger is transplanted the test tube slant, cultivated 2-3 days in 30 degree.Storage is standby after waiting to grow brown spore;
B, inoculation: the test tube slant that will cultivate 2-3 days takes a morsel under aseptic condition in spore access triangular flask wheat bran and the poor substratum of smoked unstrained spirits;
C, triangular flask wheat bran cultivate: in the incubators of 30 degree, cultivated about 3 days, treat that spore covers with after, standby
D, the cultivation of ventilating: under ventilation, stirring condition, fermented about 40-50 hour, measure enzyme and live.
Four, test-results
Through ten batches test, the result is as follows for the diastatic activity of its two kinds of raw materials (U/g):
Figure A20091014194000061
Five, experiment conclusion
Prove the contrast experiment of identical conditions that through two kinds of raw materials smoked vinegar is poor can produce wheat bran, and the enzyme work that enzyme work is done substratum than wheat bran has improved more than 30%.
In a word, the present invention utilize first can not reuse in the system vinegar smoked vinegar poor, add unique nutritive medium, according to the method fermentation of koji, the wheat bran enzyme work of gained has improved more than 30%, fermentation time has shortened 6-9 hour; The bent skin of Cheng Qu is thin, and mycelia distributes wide and even, and dense bent fragrance is arranged; This method is big to the poor digestion amount of the smoked vinegar of waste, not only can solve the poor pollution on the environment of vinegar, meet national requirements for environmental protection, and the wheat bran that makes is used to make vinegar (also can be used for industries such as wine brewing), can be system vinegar enterprise and saved a large amount of production costs, reach the purpose that turns waste into wealth, have good economic benefit and social benefit.
Embodiment
Embodiment 1:
A kind of method of utilizing the smoked vinegar lees song may further comprise the steps: (1) at first makes kind of a bacterium with the black-koji mould original seed; (2) poor and add-on is that 2% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 60%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 1%, glucose 0.2%, sal epsom 0.02%, ammonium nitrate 1.0%, Repone K 0.2%, ferrous sulfate 0.005%, bean powder 0.5%, all the other are water; (3) press the good aspergillus niger strain bacterium of 3% inoculation culture of mixture weight; (4) postvaccinal material is put into ventilating fermentation pond fermentation about 40 hours, the temperature of fermentation is 30 ℃; (5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.
Embodiment 2:
A kind of method of utilizing the smoked vinegar lees song may further comprise the steps: (1) at first makes kind of a bacterium with the black-koji mould original seed; (2) poor and add-on is that 10% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 65%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 2%, glucose 1%, sal epsom 0.08%, ammonium nitrate 2.0%, Repone K 0.6%, ferrous sulfate 0.01%, bean powder 1.5%, all the other are water; (3) press the good aspergillus niger strain bacterium of 5% inoculation culture of mixture weight; (4) postvaccinal material is put into ventilating fermentation pond fermentation about 50 hours, the temperature of fermentation is 35 ℃; (5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.
Embodiment 3:
A kind of method of utilizing the smoked vinegar lees song may further comprise the steps: (1) at first makes kind of a bacterium with the black-koji mould original seed; (2) poor and add-on is that 2% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 65%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 2%, glucose 0.2%, sal epsom 0.03%, ammonium nitrate 2.0%, Repone K 0.4%, ferrous sulfate 0.008%, bean powder 1.0%, all the other are water; (3) press the good aspergillus niger strain bacterium of 3% inoculation culture of mixture weight; (4) postvaccinal material is put into ventilating fermentation pond fermentation about 50 hours, the temperature of fermentation is 32 ℃; (5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.
Embodiment 4:
A kind of method of utilizing the smoked vinegar lees song may further comprise the steps: (1) at first makes kind of a bacterium with the black-koji mould original seed; (2) poor and add-on is that 7% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 63%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 1.5%, glucose 0.8%, sal epsom 0.05%, ammonium nitrate 1.5%, Repone K 0.4%, ferrous sulfate 0.009%, bean powder 0.9%, all the other are water; (3) press the good aspergillus niger strain bacterium of 4% inoculation culture of mixture weight; (4) postvaccinal material is put into ventilating fermentation pond fermentation about 45 hours, the temperature of fermentation is 34 ℃; (5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.

Claims (1)

1, a kind of method of utilizing the smoked vinegar lees song is characterized in that may further comprise the steps:
(1) at first the black-koji mould original seed is made kind of a bacterium;
(2) poor and add-on is that 2~10% nutritive medium of the poor weight of fresh smoked vinegar mixes and is stirred into mixture fresh smoked vinegar, the moisture that adds water adjusting mixture then is 60~65%, described nutritive medium is made up of following raw materials by weight percent: corn dextrin 1~2%, glucose 0.2~1%, sal epsom 0.02~0.08%, ammonium nitrate 1.0~2.0%, Repone K 0.2~0.6%, ferrous sulfate 0.005~0.01%, bean powder 0.5~1.5%, all the other are water;
(3) press the good aspergillus niger strain bacterium of 3~5% inoculation culture of mixture weight;
(4) postvaccinal material is put into the ventilating fermentation pond and fermented about 40~50 hours, the temperature of fermentation is 30~35 ℃;
(5) at last wheat bran is promptly made in the thin airing in wheat bran shop that ferments.
CN2009101419401A 2009-06-10 2009-06-10 Method for starter propagation by utilizing smoked vinegar lees Active CN101570725B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101921685B (en) * 2010-01-11 2012-08-01 贵州省仁怀市茅台镇老伙记酒业有限公司 Production process of highly-fragrant health care liquor
CN103756918A (en) * 2014-01-16 2014-04-30 山西梁汾醋业有限公司 Protease generating strain amplification method and method for increasing content of amino-acid-state nitrogen in vinegar
CN112023066A (en) * 2020-09-04 2020-12-04 济南今朝酒业有限公司 Sterilization kettle and culture method of aspergillus niger applying same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1054362C (en) * 1996-04-29 2000-07-12 刘文治 Method for producing organic and inorganic mixed microbe fertilizer
CN1222307A (en) * 1998-11-25 1999-07-14 王燕军 Biological feed protein with high methionine content and its preparation
CN100443589C (en) * 2005-12-30 2008-12-17 湖南鸿鹰祥生物工程股份有限公司 Production of beta-amylase concentrated liquid with high conversion-rate and purity
CN101756012A (en) * 2008-10-21 2010-06-30 天津生机集团股份有限公司 Method for producing high-lysine fermented feed through fermentation of vinegar residue using two-step method
CN101875908A (en) * 2009-12-04 2010-11-03 山西三盟实业发展有限公司 Method for composite acetic acid bacterium culture and solid-state acetic acid fermentation
CN101791051B (en) * 2010-03-12 2012-06-27 山西三盟实业发展有限公司 Preparation method of compound microbial feed additive

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101921685B (en) * 2010-01-11 2012-08-01 贵州省仁怀市茅台镇老伙记酒业有限公司 Production process of highly-fragrant health care liquor
CN103756918A (en) * 2014-01-16 2014-04-30 山西梁汾醋业有限公司 Protease generating strain amplification method and method for increasing content of amino-acid-state nitrogen in vinegar
CN112023066A (en) * 2020-09-04 2020-12-04 济南今朝酒业有限公司 Sterilization kettle and culture method of aspergillus niger applying same

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Denomination of invention: Method for starter propagation by utilizing smoked vinegar lees

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