CN101544814B - Degradable blood vessel casting agent - Google Patents
Degradable blood vessel casting agent Download PDFInfo
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- CN101544814B CN101544814B CN200910061702XA CN200910061702A CN101544814B CN 101544814 B CN101544814 B CN 101544814B CN 200910061702X A CN200910061702X A CN 200910061702XA CN 200910061702 A CN200910061702 A CN 200910061702A CN 101544814 B CN101544814 B CN 101544814B
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Abstract
The invention relates to a degradable blood vessel casting agent. The casting agent consists of casting material and an organic solvent. 1ml of the casting agent contains 0.05 to 0.5g of the casting material. The casting material is one of polylactic acid, polyglycollic acid and polycaprolactone or the combination of any two; and the organic solvent is acetone, ethyl acetate, chloroform or 1,4-dioxane. The preparation and application process of the casting agent is simple and easily operated. The concentration of the casting agent is timely regulated as required; and the casting agent can be used for feeding a cast many times during casting so as to maintain the natural form of a blood vessel maximally. After being injected into the blood vessel or a blood vessel die, the casting agent can be completely solidified through a plurality of drying methods; and then a blood vessel cast is obtained after the corrosive removal of tissues and cleaning or after opening the blood vessel die. The blood vessel cast prepared from the casting agent has certain toughness and mechanical strength. The casting agent has good biocompatibility, easy molding, and easy percolation and dissolution, and is suitable for the study on the preparation of a hepatic tissue engineering support with a physiological shape blood vessel network.
Description
Technical field
The present invention relates to a kind of degradable blood vessel casting agent.Belong to technical field of bioengineering.
Background technology
The blood vessel casting technology is a technical skill of anatomy preparation of specimen, is widely used in the preparation of specimen of blood vessel network model in animal and human's body organ, has very high using value in medical teaching and scientific research.At present, with this manufacturing technology and computer combined utilization, synthesize the blood vessel network 3-D view and be applied to the tissue engineering bracket research field, but being applied to the preparation of tissue engineering bracket, the blood vessel network 3-D view that this method obtains also need special molding device to finish, so be subjected to certain restriction because of research complex process, cost are high.
Cast agent is that its thing is injected in blood vessel, and it is back with suitable solution corrosion tissue and removing, the weighting material casting mold sample that is only stayed to treat that cast agent solidifies.Used cast agent is used nitrocellulose (zylonite) to fill out usually and is done agent in the blood vessel casting technology, the blood vessel casting of preparation internal organs.Along with developing by leaps and bounds of chemical industry, a lot of novel cast agents have appearred, as tooth shedding, ABS engineering plastics, methyl methacrylate, polyvinyl chloride and chlorinated polyvinyl chloride etc.These cast agents be used for the vascular pattern sample the depositing of suitable long period of making, be difficult for degraded, and be beneficial to computer simulation compositing 3 d images etc., but also there are some problems in the preparation that is directly used in tissue engineering bracket.
So, develop a kind of novel good biocompatibility, be easy to the cast agent of blood vessel casting, and be very important with the preparation that simple method is directly used in tissue engineering bracket.
The aliphatic poly ester material is the good bio-medical material of a class degradable biocompatibility, easily is dissolved in the multiple organic solvent.This class material is used for blood vessel casting as a kind of cast agent, and with the vascular pattern of its acquisition and support body material by the combining method of infiltration forming and lyophilize/leaching technology, can prepare tissue engineering bracket with blood vessel network.The vascular pattern of using this cast agent casting mold is suitable for preparing the research of the liver tissue engineering scaffold with physiology shape blood vessel network most, but because of having the degradable performance after the cast agent curing, is not suitable for making the vascular pattern sample of prolonged preservation.
Summary of the invention
The object of the invention aims to provide a kind of degradable type cast agent, and satisfied preparation has the needs of the tissue engineering bracket of physiology shape blood vessel network structure, overcome blood vessel casting agent commonly used difficulty be directly used in problem such as preparation tissue engineering bracket etc.This cast agent good biocompatibility, technology are simple, processing ease and can in time regulate the cast agent strength of solution as required, can carry out repeatedly after-teeming in the casting mold, in the hope of keeping the blood vessel original appearance to greatest extent; Cast agent can reach completely solidified by multiple drying means after injecting blood vessel or blood vessel mould, and has certain toughness and mechanical strength; Vascular pattern that goes out with this cast agent casting mold and support body material can be prepared the liver tissue engineering scaffold with physiology shape blood vessel network by the combining method of infiltration forming and lyophilize/leaching technology.
Realize the technical scheme of the object of the invention:
A kind of degradable blood vessel casting agent, it is made up of mold material and organic solvent, cast agent contains mold material 0.05～0.5 gram for every milliliter, wherein said mold material is any one or two kinds of mixing in degradation material poly(lactic acid), polyglycolic acid, the poly-second lactone, organic solvent is acetone, ethyl acetate, trichloromethane or 1, the 4-dioxane.
The molecular weight of described mold material is 10～800,000 D/mol.
The using method of degradable blood vessel casting agent of the present invention: before blood vessel casting, a kind of mold material or two kinds of mold materials mixing are dissolved in the organic solvent, be made into the cast agent that the quality volumetric concentration is 0.05～0.5g/ml, two kinds of mold materials mix by 5～95: 95～5 mass ratio, cast agent injects blood vessel, after seasoning, lyophilize or vacuum-drying completely solidified, with hydrochloric acid or aqueous sodium hydroxide solution corrosion structure, to organize again and remove and clean up, promptly get blood vessel casting; Perhaps cast agent is injected the blood vessel mould, after seasoning, lyophilize or vacuum-drying completely solidified, open mould and therefrom take out the solidified vascular pattern, promptly get blood vessel casting, described blood vessel mould is put into mould earlier-70～-10 ℃ and is placed 0.5～5h before injecting cast agent.Described blood vessel mould is that paraffin, gypsum, tetrafluoroethylene or stainless material are made.
The hydrochloric acid that above-mentioned corrosion structure is used or the concentration of aqueous sodium hydroxide solution are not strict with, and when the hydrochloric acid of usefulness or naoh concentration hanged down, the time that corrosion needs was long, and when concentration was high, the time that corrosion needs was just short.General hydrochloric acid mass concentration is 2%～30%, and the sodium hydroxide mass concentration is 2%～60% all can.
The advantage of degradable blood vessel casting agent of the present invention is as follows:
1. good consistency is arranged.The aliphatic polyester series material has excellent biological compatibility, can violent chemical reaction not take place and cause destroying original blood vessel pipeline with tissue.
2. cast agent concentration adjustability.Requirement according to casting mold preparation of specimen, select suitable organic solvent for use, be easy to regulate the aliphatic polyester series cast agent of different concns at normal temperatures, satisfy the requirement of blood vessel casting, in the hope of keeping the blood vessel original appearance to greatest extent, to pouring into the fine vascular link of easy omission, can carry out repeatedly after-teeming.Cast agent can adopt multiple exsiccant method to solidify, and places seasoning, lyophilize or vacuum-drying etc. as room temperature behind casting mold.
3. favorable mechanical performance and physicals are arranged.The aliphatic polyester series cast agent has excellent plasticity, makes the original appearance that can keep blood vessel when using preferably, and than being easier to enter the more tiny blood vessel of diameter.
Degradable blood vessel casting agent is applicable to the blood vessel casting of various different diameters.
Description of drawings
The rat liver part vascular tree model of Fig. 1 poly(lactic acid) cast agent preparation
Embodiment
Embodiment 1
Get 1.0 gram molecular weights and be 390,000 poly-D, L lactic acid places beaker, adds 10 milliliters of ethyl acetate, treats poly-D, after L lactic acid dissolves fully, promptly gets the poly(lactic acid) cast agent.Can be used as blood vessel casting uses.
With this cast agent rat is carried out live body liver vessel casting mold, after downcutting liver placement natural drying at room temperature then, place 25% hydrochloric acid soln corrosion 3 days, slowly wash with flowing water, remove cell and reticular tissue, soaked 1 day with suds then, promptly obtain milky rat liver vascular tree model.Its photo is seen Fig. 1.
Embodiment 2:
Get 2.0 the gram polyglycolic acids place beaker, add 10 milliliters of acetone, treat that polyglycolic acid dissolves fully after, promptly get the polyglycolic acid cast agent.Can be used as blood vessel casting uses.
Embodiment 3
Get the poly-D of 0.5 gram, L lactic acid and 0.5 gram polyglycolic acid place beaker, add 5 milliliters of trichloromethanes, and 1,4 dioxane is treated poly-D, after L lactic acid and polyglycolic acid dissolve fully, and promptly poly-D, L lactic acid and polyglycolic acid cast agent can be used as blood vessel casting and use.
Embodiment 4
Get 0.5 gram molecular weight and be 390,000 poly-D, L lactic acid places beaker, adds 10 milliliters of ethyl acetate, treats poly-D, after L lactic acid dissolves fully, promptly get the poly(lactic acid) cast agent, and every milliliter of cast agent contains poly-D, and L lactic acid 0.05 restrains.Can be used as blood vessel casting uses
Embodiment 5
Get 2.5 gram molecular weights and be 390,000 poly-D, L lactic acid places beaker, adds 5 milliliter 1, and the 4-dioxane is treated poly-D, after L lactic acid dissolves fully, promptly get the poly(lactic acid) cast agent, and every milliliter of cast agent contains poly-D, and L lactic acid 0.5 restrains.Can be used as blood vessel casting uses.
With this cast agent rat is carried out live body liver vessel casting mold, downcut liver then and put cryogenic freezing, after carrying out lyophilize again, place 25% hydrochloric acid soln corrosion 3 days, slowly wash with flowing water, remove cell and reticular tissue, soaked 1 day with suds then, promptly obtain rat liver vascular tree model.
Claims (4)
1. degradable blood vessel casting agent, it is characterized in that, this cast agent is made up of mold material and organic solvent, cast agent contains mold material 0.05～0.5 gram for every milliliter, wherein said mold material is mixing any one or two kinds of in degradation material poly(lactic acid), the polyglycolic acid, organic solvent is acetone, ethyl acetate, trichloromethane or 1, the 4-dioxane; The molecular weight of described mold material is 10～800,000.
2. the using method of the described degradable blood vessel casting agent of claim 1, it is characterized in that, before blood vessel casting, a kind of mold material or two kinds of mold materials mixing are dissolved in the organic solvent, be made into the cast agent that the quality volumetric concentration is 0.05～0.5g/ml, two kinds of mold materials mix by 5～95: 95～5 mass ratio, cast agent is injected blood vessel, after seasoning, lyophilize or vacuum-drying completely solidified, with hydrochloric acid or aqueous sodium hydroxide solution corrosion structure, to organize again and remove and clean up, promptly get blood vessel casting.
3. the using method of the described degradable blood vessel casting agent of claim 1, it is characterized in that, before blood vessel casting, a kind of mold material or two kinds of mold materials mixing are dissolved in the organic solvent, be made into the cast agent that the quality volumetric concentration is 0.05～0.5g/ml, two kinds of mold materials mix by 5～95: 95～5 mass ratio, cast agent is injected the blood vessel mould, through seasoning, open mould after lyophilize or the vacuum-drying completely solidified and therefrom take out the solidified vascular pattern, promptly get blood vessel casting, the blood vessel mould is put into mould earlier-70～-10 ℃ and is placed 0.5～5h before injecting cast agent.
4. the using method of degradable blood vessel casting agent according to claim 3 is characterized in that, described blood vessel mould is that paraffin, gypsum, tetrafluoroethylene or stainless material are made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910061702XA CN101544814B (en) | 2009-04-21 | 2009-04-21 | Degradable blood vessel casting agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910061702XA CN101544814B (en) | 2009-04-21 | 2009-04-21 | Degradable blood vessel casting agent |
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| Publication Number | Publication Date |
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| CN101544814A CN101544814A (en) | 2009-09-30 |
| CN101544814B true CN101544814B (en) | 2011-10-19 |
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| CN200910061702XA Expired - Fee Related CN101544814B (en) | 2009-04-21 | 2009-04-21 | Degradable blood vessel casting agent |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106859814B (en) * | 2017-03-13 | 2018-05-08 | 上海市东方医院 | A kind of method of 3D printing manufacture artificial blood vessel |
| CN110689790A (en) * | 2019-10-21 | 2020-01-14 | 韦义萍 | Manufacturing method of rabbit pulmonary blood vessel model |
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- 2009-04-21 CN CN200910061702XA patent/CN101544814B/en not_active Expired - Fee Related
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Granted publication date: 20111019 Termination date: 20130421 |