CN101538543A - Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof - Google Patents
Composite microbial inoculum for treating micro polluted source water under low temperature condition and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a composite microbial inoculum for treating micro polluted source water under the low temperature condition and a preparation method thereof, in particular to a composite microbial inoculum and a preparation method thereof, aiming at solving the problems that the existing microbial inoculum has low biological activity and slow growth velocity under the low temperature condition; when organic matter and ammonia nitrogen are treated in the water, organic and toxic substances are difficult to degrade, and nitrite and nitrate are accumulated; and secondary pollution for the environment is caused by the generated N2O. The product composite microbial inoculum is made of acinetobacter lwoffiiSRA10, acinetobacter calcoaceticus PHEA-2, bacillus megatherium X2 and fluid nutrient medium. The method comprises: 1. activation treatment is carried out on raw strain; 2. accumulation culture; 3. after being mixed according to the volume ratio, the strain is added into the fluid nutrient medium; finally, the composite microbial inoculum is obtained. The obtained composite microbial inoculum has high biological activity and fast growth and can be used for treating the micro polluted source water with remarkable effect under the low temperature condition; furthermore, the nitrite and the nitrate are not accumulated, and no N2O is generated, so that the composite microbial inoculum is safe and reliable.
Description
Technical field
The present invention relates to a kind of composite fungus agent and preparation method thereof.
Background technology
In recent years, along with the increase of China's industrial expansion and agrochemicals, the drinking water source is subjected to severe contamination, and is development trend; Micro-polluted source water is meant and is subjected to Organic pollutants, makes the index of some projects surpass hygienic standard.Pollutant kind contained in this class water is more, character is complicated, and trade effluent and sanitary sewage that its water quality is mainly entered influence, and shows as ammonia nitrogen, total phosphorus, colourity, organism equal size at the rivers waterhead area and exceeds standard.On the lake and reservoir water source, show as the eutrophication of reservoir and water body in lake, and give birth in certain period algae evil, cause water quality deterioration, stench pressing when rotting.At present, biologic treating technique is simple to operate because of having, operation is convenient, it is little that subsequent treatment process is influenced, and operational and administrative expenses increases few, thereby is used widely.But because micro-polluted raw self water quality characteristic, biological activity is low under cold condition, the speed of growth is slow for feasible existing microbial inoculum; Have organic, Toxic difficult degradation when organism and ammonia nitrogen in treating water, nitrite and Nitrate Accumulation phenomenon have harm to human body in the water, and can produce N
2O causes secondary pollution to environment.
Summary of the invention
The objective of the invention is that biological activity is low under cold condition, the speed of growth is slow in order to solve existing microbial inoculum; Have organic, Toxic difficult degradation when organism and ammonia nitrogen in treating water, nitrite and Nitrate Accumulation phenomenon in the water produce N
2O causes secondary pollution problem to environment, and composite fungus agent for the treatment of micro polluted source water under low temperature condition and preparation method thereof is provided.
Cold condition is removed the composite fungus agent of micro-polluted source water down and is made by Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2, bacillus megaterium (Bacillusmegaterium) X2 and liquid nutrient medium; Wherein Acinetobacter lwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; Wherein Acinetobacter lwoffiiSRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 being inoculated in respectively and being cultured to bacterial count in the liquid nutrient medium is 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacterlwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed.
The preparation method of the composite fungus agent for the treatment of micro polluted source water under low temperature condition realizes according to the following steps: one, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2 and bacillus megaterium (Bacillus megaterium) X2 are inoculated on the solid medium respectively and activate; Two, single bacterium colony of activating of picking is inoculated in respectively on the liquid nutrient medium, is that 15～20 ℃, hunting speed are enrichment culture 1～3 day under the aerobic condition of 150～170r/min in temperature; Three, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are inoculated in respectively to be cultured to bacterial count in the liquid nutrient medium be 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacter lwoffiiSRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed, and promptly gets the composite fungus agent for the treatment of micro polluted source water under low temperature condition; Wherein Acinetobacterlwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation in the step 1, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; Wherein solid medium is that every 1000mL soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 1
2HPO
4, 1～5g KH
2PO
4, 2% agar, the NH of 3～10g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 2
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0.
The composite fungus agent that obtains among the present invention still has higher biological activity under low temperature (2～5 ℃) condition (dehydrogenase activity is 40～45mgTF/Lh); Show the trend that is quick growth through recording composite fungus agent production rate behind 8h.The composite fungus agent that the present invention obtains under cold condition (2～10 ℃) to micro-polluted source water in organism have stronger degradation capability (maximum material removal rate be 81%, average removal rate is 68%), simultaneously can degradation of ammonia nitrogen (maximum material removal rate is 86%, and average removal rate is 70%); The composite fungus agent environmental change resistivity to external world that the present invention obtains is strong, and the composite fungus agent that the present invention obtains is applied in the biological treatment system, can shorten the start time of biological treatment system, plays the ideal process effect in short duration; The composite fungus agent that the present invention obtains is easy to use, and operational administrative is simple, and effect is obvious, and water outlet nitrate and nitrites do not accumulate, and does not produce N
2O is safe and reliable.
SRA10 among the present invention in the composite fungus agent for the treatment of micro polluted source water under low temperature condition is Shandong luxuriant and rich with fragrance acinetobacter calcoaceticus Acinetobacter lwoffii, belong to acinetobacter (Acinetobacter), in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009.
Description of drawings
Fig. 1 handles the graphic representation of Song Hua River sewage to ammonia nitrogen removal frank for the composite fungus agent that embodiment 11 obtains treating micro polluted source water under low temperature condition, Fig. 2 handles the graphic representation of Song Hua River sewage to organic removal rate for the composite fungus agent that embodiment 11 obtains treating micro polluted source water under low temperature condition, among the figure
In influent ammonium concentration, figure
In water outlet ammonia nitrogen concentration, figure
In water outlet nitrite concentration, figure
Water outlet nitrate concentration, Fig. 3 obtain the composite fungus agent growth curve chart for the treatment of micro polluted source water under low temperature condition for embodiment 11.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the composite fungus agent of present embodiment treating micro polluted source water under low temperature condition is made by Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2, bacillus megaterium (Bacillus megaterium) X2 and liquid nutrient medium; Wherein Acinetobacterlwoffii SRA10 is in China Committee for Culture Collection of Microorganisms common micro-organisms center (Datun Road, Chaoyang District, Beijing City) preservation, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; Wherein Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 being inoculated in respectively and being cultured to bacterial count in the liquid nutrient medium is 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed.
Acinetobacter calcoaceticus in the present embodiment (Acinetobacter calcoaceticus) PHEA-2 belongs to acinetobacter (Acinetobacter), this bacterium is recorded in 3 phases of " China Environmental Science " calendar year 2001 " Acinetobacter calcoaceticus PHEA-2 Pyrogentisinic Acid's the degradation characteristic research " literary composition, and this bacterial strain can be buied by " Acinetobacter calcoaceticus PHEA-2 Pyrogentisinic Acid's degradation characteristic research " author place.
Bacillus megaterium in the present embodiment (Bacillus megaterium) X2 belongs to bacillus (Bacillus), this bacterium is recorded in " Agricultural University Of Shenyang's journal " 2006 4 phases 607～610 " bacillus megaterium is to aquaculture water ammonia nitrogen degradation characteristic research " literary composition, and this bacterial strain can be buied by " bacillus megaterium is to aquaculture water ammonia nitrogen degradation characteristic research " author place.
Luxuriant and rich with fragrance acinetobacter calcoaceticus in Shandong in the present embodiment in the composite fungus agent for the treatment of micro polluted source water under low temperature condition and Acinetobacter calcoaceticus are according to " the outstanding Bacteria Identification handbook of uncle is judged to be acinetobacter.
Embodiment two: present embodiment and embodiment one are different is that the volume ratio of Acinetobacter lwoffiiSRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 is 2: 3: 2.Other is identical with embodiment one.
Embodiment three: what present embodiment and embodiment one were different is that the every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 3g, 5g, the Na of 0.9g by the Tryptones of 5g, the yeast of 3g
2HPO
4, 2g KH
2PO
4, 5g NH
4The water of Cl and surplus is formed, and the pH value is 7.0.Other is identical with embodiment one.
Embodiment four: the preparation method of the composite fungus agent of present embodiment treating micro polluted source water under low temperature condition realizes according to the following steps: one, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2 and bacillus megaterium (Bacillus megaterium) X2 are inoculated on the solid medium respectively and activate; Two, single bacterium colony of activating of picking is inoculated in respectively on the liquid nutrient medium, is that 15～20 ℃, hunting speed are enrichment culture 1～3 day under the aerobic condition of 150～170r/min in temperature; Three, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are inoculated in respectively to be cultured to bacterial count in the liquid nutrient medium be 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed, and promptly gets the composite fungus agent for the treatment of micro polluted source water under low temperature condition; Wherein Acinetobacter lwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation in the step 1, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; Wherein solid medium is that every 1000mL soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 1
2HPO
4, 1～5g KH
2PO
4, 2% agar, the NH of 3～10g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 2
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0.
Solid medium uses behind the sterilization 30min down at 121 ℃ in the present embodiment step 1.
Liquid nutrient medium in present embodiment step 2 and the step 3 uses behind the sterilization 30min down at 121 ℃.
The composite fungus agent that present embodiment obtains is 0～10 ℃ of preservation; The composite fungus agent total plate count that obtains is greater than 10
9CFU/ml.
Embodiment five: what present embodiment and embodiment four were different is that solid medium is that every 1000mL soaks the NaCl of the extractum carnis of powder, 3g, 5g, the Na of 0.9g by the Tryptones of 5g, the yeast of 3g in the step 1
2HPO
4, 2g KH
2PO
4, 2% agar, the NH of 5g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0.Other step and parameter are identical with embodiment four.
Embodiment six: what present embodiment and embodiment four were different is that the every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 3g, 5g, the Na of 0.9g by the Tryptones of 5g, the yeast of 3g in the step 2
2HPO
4, 2g KH
2PO
4, 5g NH
4The water of Cl and surplus is formed, and the pH value is 7.0.Other step and parameter are identical with embodiment four
Embodiment seven: what present embodiment and embodiment four to six were different is that hunting speed is 155～165r/min in the step 2.Other step and parameter are identical with embodiment four to six.
Embodiment eight: what present embodiment and embodiment four to six were different is that hunting speed is 160r/min in the step 2.Other step and parameter are identical with embodiment four to six.
Embodiment nine: present embodiment and embodiment four to eight are different is in the step 3 Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 to be inoculated in respectively that to be cultured to bacterial count in the liquid nutrient medium be 10
9Individual/mL, 10
9Individual/mL, 10
9Individual/mL.Other step and parameter are identical with embodiment four to eight.
Embodiment ten: what present embodiment and embodiment four to nine were different is in the step 3 Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 to be mixed by 2: 3: 2 volume ratio.Other step and parameter are identical with embodiment four to nine.
Embodiment 11: the preparation method of the composite fungus agent of present embodiment treating micro polluted source water under low temperature condition realizes according to the following steps: one, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2 and bacillus megaterium (Bacillus megaterium) X2 are inoculated on the solid medium respectively and activate; Two, single bacterium colony of activating of picking is inoculated in respectively on the liquid nutrient medium, is that 18 ℃, hunting speed are enrichment culture 2 days under the aerobic condition of 160r/min in temperature; Three, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are inoculated in respectively to be cultured to bacterial count in the liquid nutrient medium be 10
9Individual/mL, 10
9Individual/mL, 10
9Individual/mL, then Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 mix by 2: 3: 2 volume ratios, promptly get the composite fungus agent for the treatment of micro polluted source water under low temperature condition; Wherein Acinetobacter lwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation in the step 1, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.8 μ m, wide 1.4 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 2.0 μ m, wide be 1.2 μ m, ovalize, middle life or the life of inferior end; Solid medium is that every 1000mL soaks the NaCl of the extractum carnis of powder, 3g, 5g, the Na of 0.9g by the Tryptones of 5g, the yeast of 3g
2HPO
4, 2g KH
2PO
4, 2% agar, 5g NH
4The water of Cl and surplus is formed, and the pH value is 7.0; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 3g, 5g, the Na of 0.9g by the Tryptones of 5g, the yeast of 3g in the step 2
2HPO
4, 2g KH
2PO
4, 5g NH
4The water of Cl and surplus is formed, and the pH value is 7.0.
The composite fungus agent of the treating micro polluted source water under low temperature condition that present embodiment obtains purifies the river of gathering from waters, Song Hua River, Heilongjiang Province, under 2～8 ℃ condition, detect ammonia nitrogen content through the nessler reagent light-intensity method, as shown in Figure 1, move that ammonia nitrogen removal frank is that maximum material removal rate is 81% after 40 days, average removal rate is 68%, and the water outlet ammonia nitrogen concentration is 0～1mg/L; Through recording as shown in Figure 2, move that organic removal rate is that maximum material removal rate is 86% after 40 days, average removal rate is 70%, and nitrate-free and nitrite accumulation, does not produce N
2O and water outlet COD
MnBe 0～3mg/L.By Fig. 1 and Fig. 2 can be clear and definite find out that the composite fungus agent that the present invention obtains has the ideal treatment effect to micro-polluted source water under cold condition.
The composite fungus agent of the treating micro polluted source water under low temperature condition that present embodiment obtains is 3 ℃ in temperature, the pH value is 7, rotating speed be under the condition of 170r/min growth curve as shown in Figure 3, as can be seen from Figure 3 the speed of growth shows the trend that is quick growth behind the composite fungus agent 8h.
Claims (4)
1, the composite fungus agent for the treatment of micro polluted source water under low temperature condition is characterized in that the composite fungus agent for the treatment of micro polluted source water under low temperature condition is made by Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2, bacillus megaterium (Bacillus megaterium) X2 and liquid nutrient medium; Wherein Acinetobacter lwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; Wherein Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 being inoculated in respectively and being cultured to bacterial count in the liquid nutrient medium is 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacter lwoffiiSRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed.
2, prepare the composite fungus agent method for the treatment of micro polluted source water under low temperature condition according to claim 1, it is characterized in that the preparation method of the composite fungus agent for the treatment of micro polluted source water under low temperature condition realizes according to the following steps: one, Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) PHEA-2 and bacillus megaterium (Bacillus megaterium) X2 are inoculated on the solid medium respectively and activate; Two, single bacterium colony of activating of picking is inoculated in respectively on the liquid nutrient medium, is that 15～20 ℃, hunting speed are enrichment culture 1～3 day under the aerobic condition of 150～170r/min in temperature; Three, Acinetobacterlwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are inoculated in respectively to be cultured to bacterial count in the liquid nutrient medium be 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, 10
9～10
10Individual/mL, then Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 are by 1～3: 2～4: 1～3 volume ratio is mixed, and promptly gets the composite fungus agent for the treatment of micro polluted source water under low temperature condition; Wherein Acinetobacter lwoffii SRA10 is in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation in the step 1, and deposit number is CGMCC No.2889, and preservation date is on January 19th, 2009; Acinetobacter calcoaceticus PHEA-2 quarter butt or sphere, long 1.5～2.0 μ m, wide 1.0～1.5 μ m, no gemma and flagellum, the motion of twitching; Bacillus megaterium X2 is shaft-like, and terminal circle is single or is short chain and arranges, the motion of twitching, brood cell's length be 1.5～2.0 μ m, wide be 1.0～1.2 μ m, ovalize, middle life or the life of inferior end; Wherein solid medium is that every 1000mL soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 1
2HPO
4, 1～5g KH
2PO
4, 2% agar, the NH of 3～10g
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0; The every 1000mL of liquid nutrient medium soaks the NaCl of the extractum carnis of powder, 2～8g, 3～9g, the Na of 0.5～2g by the Tryptones of 2～7g, the yeast of 1～5g in the step 2
2HPO
4, 1～5g KH
2PO
4, 3～10g NH
4The water of Cl and surplus is formed, and the pH value is 6.0～8.0.
3, the preparation method of the composite fungus agent for the treatment of micro polluted source water under low temperature condition according to claim 2 is characterized in that hunting speed is 155～165r/min in the step 2.
4,, it is characterized in that Acinetobacter lwoffii SRA10, Acinetobacter calcoaceticus PHEA-2 and bacillus megaterium X2 mix by 2: 3: 2 volume ratios in the step 3 according to the preparation method of the composite fungus agent of claim 2 or 3 described treating micro polluted source water under low temperature condition.
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| CN103103140A (en) * | 2011-11-09 | 2013-05-15 | 浙江商达环保有限公司 | Aerobic phosphorus removing bacterial stain for sewage treatment and application thereof |
| CN108723084A (en) * | 2018-04-12 | 2018-11-02 | 江苏世邦生物工程科技有限公司 | Microorganism formulation and its preparation method and application for low temperature soil reparation |
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| CN1657606A (en) * | 2005-01-28 | 2005-08-24 | 中国科学院南京土壤研究所 | Method for repairing soil at high-efficient power of bacterial to emulsify petroleum |
| CN101182093B (en) * | 2007-12-07 | 2010-06-23 | 陈五岭 | Microbe harmless treatment method for oil-gas field waste slurry |
| CN101255404B (en) * | 2008-02-26 | 2010-12-15 | 新疆农业科学院微生物应用研究所 | Bacteria for producing ephedrine and ephedrine produced thereby |
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| CN103103140A (en) * | 2011-11-09 | 2013-05-15 | 浙江商达环保有限公司 | Aerobic phosphorus removing bacterial stain for sewage treatment and application thereof |
| CN102794296A (en) * | 2012-08-01 | 2012-11-28 | 中国石油天然气股份有限公司 | Complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and restoring method thereof |
| CN102794296B (en) * | 2012-08-01 | 2013-07-31 | 中国石油天然气股份有限公司 | Complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and restoring method thereof |
| CN108723084A (en) * | 2018-04-12 | 2018-11-02 | 江苏世邦生物工程科技有限公司 | Microorganism formulation and its preparation method and application for low temperature soil reparation |
| CN109609431A (en) * | 2018-12-29 | 2019-04-12 | 哈尔滨工业大学 | A method of promote fast-growth and activity under the motionless bacterium low temperature in Harbin to improve |
| CN110982732A (en) * | 2019-11-13 | 2020-04-10 | 重庆理工大学 | Salt-tolerant high-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification composite microbial agent and preparation and application thereof |
| CN110982732B (en) * | 2019-11-13 | 2022-12-30 | 重庆理工大学 | Salt-tolerant and high-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification composite microbial agent and preparation and application thereof |
| CN112813005A (en) * | 2021-02-09 | 2021-05-18 | 黑龙江大学 | Biological agent for strengthening treatment of humic acid in water and preparation method and application thereof |
| CN112813005B (en) * | 2021-02-09 | 2023-01-06 | 黑龙江大学 | Biological agent for strengthening treatment of humic acid in water and preparation method and application thereof |
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