CN101531989A - Method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol - Google Patents
Method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol Download PDFInfo
- Publication number
- CN101531989A CN101531989A CN200910082045A CN200910082045A CN101531989A CN 101531989 A CN101531989 A CN 101531989A CN 200910082045 A CN200910082045 A CN 200910082045A CN 200910082045 A CN200910082045 A CN 200910082045A CN 101531989 A CN101531989 A CN 101531989A
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- China
- Prior art keywords
- pichia stipitis
- fermentation
- tree trunk
- inner ether
- growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention introduces a method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol. According to the method, 0.1% of anhydro-sugar is added in the process of culturing and fermentation of the tree trunk pichia stipitis, which can promote growth of the tree trunk pichia stipitis by 8% and increase production of the fermented glucose ethanol by 25%.
Description
Technical field
The invention belongs to microorganism culturing and alcohol production technical field, relate to one and promote the growth of pichia stipitis bacterium and the method for fermentation producing and ethanol.
Technical background
Inner ether sugar (levoglucosan, 1,6-dehydration-β-D-Glucopyranose) is a cellulose pyrolytic product, and it is mainly used in the intermediate product of chemosynthesis at present.Discover that most of eukaryotic microorganisms can utilize the energy and the carbon source of inner ether sugar as growth.Its mechanism is that eukaryotic microorganisms passes through a kind of inner ether sugar kinases, the inner ether sugar catalytic hydrolysis is become the 6-glucose 1-phosphate1-, and directly enter the glycolytic pathway utilization.The inner ether sugar kinases is an inducible enzyme.Korean (Hai Quoc Luyen et al.) finds that a spot of inner ether sugar can promote the growth of chlorella, and increment improves up 150%.
In the last few years, because fossil energies such as oil is in short supply, produced alcohol fuel by Mierocrystalline cellulose and extensively launched research just in the world.At present an important production method of cellulosic ethanol is to adopt cellulosic acid hydrolysis and the microbial fermentation technology that combines.Cellulosic acid hydrolysis products contains glucose and wood sugar etc., but the unfermentable wood sugar of S. cervisiae, and pichia stipitis bacterium (Pichia stipitis) can glucose fermentation and wood sugar producing and ethanol.Adopting the acid hydrolysis products production alcohol fuel of pichia stipitis bacterium fermented cellulose is an ideal method.
Yet, also do not have in the cultivation and fermenting process that is reported in the pichia stipitis bacterium at present, add other material with the growth of promotion pichia stipitis bacterium and the method for ethanol production.Be subjected to inner ether sugar can promote the inspiration of the growth of chlorella, we find that inner ether sugar can promote the growth of pichia stipitis bacterium, and can improve alcoholic acid output.
Summary of the invention
The purpose of this invention is to provide a kind ofly in the incubation growth and fermenting process of pichia stipitis bacterium, add inner ether sugar with the growth that promotes the pichia stipitis bacterium and the method for fermentation producing and ethanol.
1, inner ether sugar promotes the growth of pichia stipitis bacterium
In the YPD liquid nutrient medium, inoculum size is 1%, adds 0.1% inner ether sugar, and 30 ℃, 200 rev/mins, the increment of pichia stipitis bacterium can improve 8% than the sample that does not add inner ether sugar after 6 hours.
2, inner ether sugar promotes the fermentation producing and ethanol output of pichia stipitis bacterium
In the YPD liquid nutrient medium, inoculum size is 1%, adds 0.1% inner ether sugar, 30 ℃, and 130 rev/mins.After 72 hours, measure ethanol production than the fermentation that does not add inner ether sugar and improve 25%.
Claims (6)
1. in the YPD liquid nutrient medium, cultivate pichia stipitis bacterium (Pichia stipitis).
2. in 1 described method of claim the, add 0.1% inner ether sugar.
3. the described feature of claim 2 is the increment raising 8% from Pichia yeast.
4. in the YPD liquid fermentation medium, inoculate the pichia stipitis bacterium.
5. in 4 described methods of claim the, add 0.1% inner ether sugar.
6. the described feature of claim 5 is to improve 25% from Pichia yeast fermentation producing and ethanol amount.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910082045A CN101531989A (en) | 2009-04-22 | 2009-04-22 | Method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910082045A CN101531989A (en) | 2009-04-22 | 2009-04-22 | Method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101531989A true CN101531989A (en) | 2009-09-16 |
Family
ID=41102855
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910082045A Pending CN101531989A (en) | 2009-04-22 | 2009-04-22 | Method for promoting growth and fermentation of tree trunk Pichia stipitis to produce ethanol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101531989A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109294933A (en) * | 2018-11-20 | 2019-02-01 | 北京工商大学 | One Accharomyces cerevisiae and its method for co-culturing raising traditional fermented food quality with ester-producing yeast |
| CN114317297A (en) * | 2021-11-12 | 2022-04-12 | 广西科学院 | High-yield ethanol fermentation strain and mutation breeding method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275145A (en) * | 2008-01-23 | 2008-10-01 | 中国科学院研究生院 | Cloning method for Lipomyces Starkeyi microzyme anhydro-sugar kinase genes, gene sequence thereof and use thereof |
-
2009
- 2009-04-22 CN CN200910082045A patent/CN101531989A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101275145A (en) * | 2008-01-23 | 2008-10-01 | 中国科学院研究生院 | Cloning method for Lipomyces Starkeyi microzyme anhydro-sugar kinase genes, gene sequence thereof and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| 刘健等: "木糖发酵生产工艺的研究", 《工业微生物》 * |
| 刘继开等: "代谢木糖和葡萄糖的重组酿酒酵母的构建", 《可再生资源》 * |
| 张亚云等: "树干毕赤酵母发酵木糖生产乙醇", 《酿酒》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109294933A (en) * | 2018-11-20 | 2019-02-01 | 北京工商大学 | One Accharomyces cerevisiae and its method for co-culturing raising traditional fermented food quality with ester-producing yeast |
| CN109294933B (en) * | 2018-11-20 | 2022-10-04 | 北京工商大学 | Saccharomyces cerevisiae and method for improving quality of traditional fermented food by co-culturing same with ester-producing yeast |
| CN114317297A (en) * | 2021-11-12 | 2022-04-12 | 广西科学院 | High-yield ethanol fermentation strain and mutation breeding method thereof |
| CN114317297B (en) * | 2021-11-12 | 2022-11-08 | 广西科学院 | High-yield ethanol fermentation strain and mutation breeding method thereof |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20090916 |