CN107760753A - It is a kind of to utilize the method for being pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermenting and producing butanol - Google Patents
It is a kind of to utilize the method for being pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermenting and producing butanol Download PDFInfo
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- CN107760753A CN107760753A CN201711285336.7A CN201711285336A CN107760753A CN 107760753 A CN107760753 A CN 107760753A CN 201711285336 A CN201711285336 A CN 201711285336A CN 107760753 A CN107760753 A CN 107760753A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a kind of using the method for being pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermenting and producing butanol, comprise the following steps：(1) the sugared high temperature anaerobic bacterium of pyrolysis of activation is inoculated into the fermentation medium containing xylan and fermented, obtain zymotic fluid；(2) clostridium acetobutylicum of activation is inoculated into the zymotic fluid that step (1) obtains, fermenting and producing butanol.Clostridium acetobutylicum bacterium mud is added when being pyrolyzed sugared high temperature anaerobic bacterium M5 and having cultivated 60h, butanol yield highest, reaches 8.34g/L, this, which is also, co-cultures obtained highest butanol yield when currently with xylan being sole carbon source.The cost of industrial production butanol is this method reduce, while solves the problems, such as that the sugared high temperature anaerobic bacterium of pyrolysis can but butanol yields poorly using hemicellulose, there is important application value.
Description
Technical field
The invention belongs to field of microbial fermentation, is related to butanol fermentation, and in particular to one kind utilizes and is pyrolyzed sugared high temperature anaerobic
The method that bacterium and clostridium acetobutylicum co-culture fermenting and producing butanol.
Background technology
As fuel, butanol have energy density it is big, it is high to the stability of water, can be directly used for internal combustion engine, convenient transportation
The advantages that, in today that energy crisis is increasingly serious, butanol has vast potential for future development as fuel.The production method of butanol
Mainly there are acetaldehyde condensation method, propenecarbonyl synthetic method and fermentation method etc..As ballooning oil prices and resource accelerate exhausted, fermentation
Method production butanol receives extensive attention, is increasingly becoming one of study hotspot of bioenergy.
Traditional fermentation method produces butanol mainly using grain or other starchiness agricultural byproducts as raw material, is sent out through hydrolysis
Zymotic fluid, butanol is then obtained in the presence of zymophyte, bacterial strain is mostly solvent clostridium, such as visits formula clostridium, clostridium acetobutylicum
Deng.Because it can not be carbon source using lignocellulosic, its production cost greatly improves, and is unfavorable for industrial production.Grain straw
In main component be cellulose and hemicellulose, the wherein key component of hemicellulose is xylan, can be using xylan as only
One carbon source production butanol can substantially reduce production cost, and can also turn waste into wealth.
Thermophilic anaerobic bacillus be report at present uniquely hemicellulose can directly be utilized by integral biological process engineering
The thermophilic type wild strain of element production biological butanol, but its butanol yield is relatively low, report yield is generally below 0.1g/L.
CN106995790A, which discloses one plant, to be the pyrolysis sugar high temperature anaerobic bacterium that carbon source produces butanol directly using lignocellulosic
Strain M5 (Thermoanaerobacterium thermosaccharolyticum), butanol yield is up to 1.32g/L, but its
Butanol yield is still in reduced levels.It is gram sun and solvent clostridium such as clostridium acetobutylicum is traditional production solvent clostridium
Property bacterium, movable in both ends circle rod-short, amphitrichous, its butanol yield is higher, typically can reach 10g/L or so, but its
Can only be by the use of monose as carbon source, such as glucose, xylose etc., cost is higher, leverages its industrial production.At present to utilizing
Lignocellulosic is that the report that raw material is co-cultured is less.
The content of the invention
Goal of the invention：It is produced into solve traditional butanol fermentation process using grain or other starchiness byproducts as raw material
The problem of this is too high, is utilized the invention provides one kind and be pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermenting and producing
The method of butanol.
Technical scheme：A kind of utilize of the present invention is pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermentation life
The method for producing butanol, comprises the following steps：
(1) the sugared high temperature anaerobic bacterium of pyrolysis of activation is inoculated into the fermentation medium containing xylan and fermented, obtained
To zymotic fluid；
(2) clostridium acetobutylicum of activation is inoculated into the zymotic fluid that step (1) obtains, fermenting and producing butanol.
When the enzyme activity of zytase reaches 0.3-0.8U/mL in step (1) described zymotic fluid, by the acetone-butanol shuttle of activation
Bacterium is inoculated into the zymotic fluid that step (1) obtains.Now there is a certain amount of xylose in zymotic fluid, and zytase and xylobiose enzyme
Active highest, can continuous degradation xylan be xylose, and clostridium acetobutylicum can utilize the obtained xylose production fourth of degraded
Alcohol.Inoculation time is too early, and the enzyme activity of zytase is relatively low, does not utilize the degraded of follow-up xylan；Inoculation time too late, then can be led
Cause bacterial strain M5 incubation times long, zytase and xylobiose enzyme secretion amount are reduced, and the enzyme previously secreted out of is at 55 DEG C
Enzyme activity declines, and so as to cause the enzyme activity of enzyme system to decline, and the xylose that Partial digestion obtains also is utilized by bacterial strain M5, so as to reduce
The carbon flow of clostridium acetobutylicum, ultimately result in butanol yield reduction.Therefore, the inoculation time of clostridium acetobutylicum is to co-culture
The extremely crucial step of fourth of fermenting.
Preferably, when the enzyme activity of zytase reaches 0.5-0.6U/mL in step (1) described zymotic fluid, by the third of activation
Ketone Clostridium acetobutylicum is inoculated into fermenting and producing butanol in the zymotic fluid that step (1) obtains.
The inoculum concentration of the sugared high temperature anaerobic bacterium of pyrolysis of step (1) described activation is the 1-10% of fermentation medium volume；Institute
Stating the fermentative medium formula containing xylan is：0.5-2.0g/L NaCl, 0.5-2.0g/L K2HPO4, 0.5-2.0g/L
KH2PO4, 1.0-5.0g/L dusty yeasts, 0.2-1.0g/L MgCl2·6H2O, 0.1-0.6g/L NH4Cl, 0.01-0.05g/L
CaCl2·2H2O, 0.5-2.0g/L FeCl2·4H2O, 0.1-0.5g/L KCl, xylan 30-90g/L, solvent are water, regulation
PH to 6.0-6.5, lead to nitrogen 10-20min, 121 DEG C of sterilizing 15min；The fermentation condition is：50-65 DEG C of fermentation temperature, fermentation
Time is 36-72h, and fermentation pH is 5.5-8.5 (adjusting pH every 12-24h), rotating speed 0-120rpm, can be left to ferment, can also stir
Mix fermentation.
Preferably, xylan concentration is 60g/L in the fermentation medium containing xylan.
Preferably, step (1) described fermentation condition is：55 DEG C, fermentation time 60h of fermentation temperature, fermentation pH are 7.5, are turned
Fast 120rpm.
The activation culture based formulas of the sugared high temperature anaerobic bacterium of pyrolysis is 0.5-2.0g/L NaCl, 0.5-2.0g/L
K2HPO4, 0.5-2.0g/L KH2PO4, 1.0-5.0g/L dusty yeasts, 0.2-1.0g/L MgCl2·6H2O, 0.1-0.6g/L
NH4Cl, 0.01-0.05g/L CaCl2·2H2O, 0.5-2.0g/L FeCl2·4H2O, 0.1-0.5g/L KCl, xylan 30-
90g/L, solvent are water, adjust pH to 6.0-6.5；Activation condition is：Sugared high temperature anaerobic bacterium will be pyrolyzed to connect with inoculum concentration 5%v/v
Kind 50-65 DEG C, 0-120rpm activation 48-96h, pH to 5.5-8.5 is adjusted every 12h into activation medium.
Wherein, sugared high temperature anaerobic bacterium (Thermoanaerobacterium thermosaccharolyticum), bacterium are pyrolyzed
Strain number is M5, has been stored in China typical culture collection center, and preservation date is on 2 27th, 2017, preserving number CCTCC
NO:M 2017072, preservation address are：Chinese Wuhan Wuhan Universitys, it has been disclosed that apply for a patent CN 106995790A in China
In.Xylose can be obtained by zytase degradation of xylan by being pyrolyzed sugared high temperature anaerobic bacterium M5, and in xylose isomerase and wooden ketone
3-P- glyceraldehyde is obtained in the presence of sugared kinases, hence into tricarboxylic acid cycle, obtains pyruvic acid, in a series of urging by enzymes
Change may finally obtain the products such as acetic acid, ethanol, butyric acid, butanol.And these enzymes all have stronger temperature tolerance, to work
Industry production has very high value.Bacterial strain M5 possesses the full gene from xylose to butanol, and itself generation without acetone, is
That reports so far uniquely directly can produce butanol and the wild strain generated without acetone, but its butanol yield using xylan
It is relatively low, optimize by culture medium etc., highest butanol yield only has 1.32g/L, still in reduced levels.In addition, bacterial strain M5
It can be grown at 50-65 DEG C, what it was secreted out of is respectively provided with higher temperature stability from xylan to the enzyme of production butanol.Institute
It is clostridium acetobutylicum (Clostridium acetobutylicum) to state clostridium acetobutylicum Classification And Nomenclature, and bacterial strain number is ATCC
824。
The vaccination ways of the clostridium acetobutylicum of step (2) described activation are as follows：By the clostridium acetobutylicum culture of activation
Liquid centrifuges, and the precipitation that centrifugation obtains is inoculated into the zymotic fluid that step (1) obtains, wherein the clostridium acetobutylicum culture activated
Liquid is identical with the fermentating liquid volume that step (1) obtains；The fermentation condition is：35-39 DEG C of fermentation temperature, fermentation time 60-
168h, fermentation pH are 4.5-7.0, rotating speed 100-200rpm.
Preferably, step (2) described fermentation condition is：37 DEG C, fermentation time 120h of fermentation temperature, fermentation pH are 5.5,
Rotating speed is 150rpm.
The activation culture based formulas of the clostridium acetobutylicum is 0.5-2.0g/L NaCl, 0.5-2.0g/L K2HPO4,
0.5-2.0g/L KH2PO4, 1.0-5.0g/L dusty yeasts, 0.2-1.0g/L MgCl2·6H2O, 0.1-0.6g/L NH4Cl,
0.01-0.05g/L CaCl2·2H2O, 0.5-2.0g/L FeCl2·4H2O, 0.1-0.5g/L KCl, xylose 30-90g/L, it is molten
Agent is water；Activation condition is as follows：Clostridium acetobutylicum is inoculated into activation medium with inoculum concentration 5%v/v, 37 DEG C,
150rpm activates 48-96h, and pH to 4.5-7.0, the clostridium acetobutylicum nutrient solution activated are adjusted every 12h.
Beneficial effect：Compared with prior art, technical advantage of the invention is as follows：
(1) present invention can utilize the pyrolysis sugar high temperature anaerobic bacterium M5 that xylan is sole carbon source growth by first cultivating,
By xylan degrading it is xylose in the case of high temperature anaerobic, but because the butanol that its production obtains yields poorly, therefore utilize butanol
Yield is higher but directly can not be co-cultured using the clostridium acetobutylicum of hemicellulose, to improve butanol yield；
(2) the addition clostridium acetobutylicum bacterium mud when being pyrolyzed sugared high temperature anaerobic bacterium M5 and having cultivated 60h, butanol yield highest,
Reach 8.34g/L, this, which is also, co-cultures obtained highest butanol yield when currently with xylan being sole carbon source.M5 is cultivated
During 60h, now in zymotic fluid zytase enzyme activity highest, zytase effectively degrades xylan, and obtains 18g/L's
Xylose；Hereafter, in co-culture system, zytase and xylobiose enzyme still have higher enzyme activity, can continually degrade
Xylan is xylose, and clostridium acetobutylicum can utilize the xylose production butanol that degraded obtains, and realize simultaneous saccharification and fermentation；
(3) cost of industrial production butanol is this method reduce, while solve the sugared high temperature anaerobic bacterium of pyrolysis to utilize half
Cellulose the problem of but butanol yields poorly, there is important application value.
Brief description of the drawings
Fig. 1 is the enzyme activity variation diagram of xylose concentration and zytase in zymotic fluid under optimization co-cultivation fermentation condition；
Fig. 2 is production concentration variation diagram in zymotic fluid under optimization co-cultivation fermentation condition.
Embodiment
Embodiment 1 is using xylose as utilization of carbon source clostridium acetobutylicum Clostridium acetobutylicum ATCC
824 production butanol
The single bacterium colonies of picking bacterial strain clostridium acetobutylicum Clostridium acetobutylicum ATCC 824 from flat board
It is inoculated into 5mL fermentation mediums, 37 DEG C, 150rpm culture 48h, new fermented and cultured is then inoculated into inoculum concentration 5%v/v
In base, 37 DEG C, 150rpm concussion and cultivates, the concentration of its various product is surveyed with GC after 12h adjusts pH to 5.5,120h, finally
Butanol yield is 8.97g/L.
Above-mentioned fermentative medium formula is 1g/L NaCl, 0.75g/L K2HPO4, 0.75g/L KH2PO4, 3g/L yeast
Powder, 0.5g/L MgCl2·6H2O, 0.3g/L NH4Cl, 0.015g/L CaCl2·2H2O, 1.5g/L FeCl2·4H2O,
0.3g/L KCl, xylose 60g/L, pH to 5.5 is adjusted, lead to nitrogen 10-20min, 121 DEG C of sterilizing 15min.
Influence of the different xylan concentration of embodiment 2 to final butanol yield
(1) sugared high temperature anaerobic bacterium will be pyrolyzed to be inoculated into activation medium with inoculum concentration 5%v/v, 55 DEG C, 120rpm activation
60h, pH to 7.5 is adjusted every 12h；
Activation culture based formulas is：1g/L NaCl, 0.75g/L K2HPO4, 0.75g/L KH2PO4, 3g/L dusty yeasts,
0.5g/L MgCl2·6H2O, 0.3g/L NH4Cl, 0.015g/L CaCl2·2H2O, 1.5g/L FeCl2·4H2O, 0.3g/L
KCl, xylan 60g/L, solvent are water, adjust pH to 7.5；
(2) the pyrolysis sugar high temperature anaerobic bacterium M5 of activation is inoculated into fermentation medium with inoculum concentration 5%v/v, 55 DEG C,
120rpm fermentation 60h, adjust pH to 7.5 every 12h, obtain zymotic fluid；
Fermentative medium formula is：1g/L NaCl, 0.75g/L K2HPO4, 0.75g/L KH2PO4, 3g/L dusty yeasts,
0.5g/L MgCl2·6H2O,0.3g/L NH4Cl,0.3g/L KCl,0.015g/L CaCl2·2H2O, 1.5g/L FeCl2·
4H2O, solvent are water, pH7.5, lead to nitrogen 10~20min, 121 DEG C of sterilizing 15min；Three groups of fermentation mediums are set, wherein wood
Glycan concentration is respectively 30g/L, 60g/L, 90g/L；
(3) clostridium acetobutylicum is inoculated into the activation medium of clostridium acetobutylicum with inoculum concentration 5%v/v, 37 DEG C,
150rpm activates 60h, and pH to 5.5 is adjusted every 12h；
Activation culture based formulas is：1g/L NaCl, 0.75g/L K2HPO4, 0.75g/L KH2PO4, 3g/L dusty yeasts,
0.5g/L MgCl2·6H2O, 0.3g/L NH4Cl, 0.015g/L CaCl2·2H2O, 1.5g/L FeCl2·4H2O, 0.3g/L
KCl, xylose 60g/L, solvent are water, adjust pH to 5.5；
(4) clostridium acetobutylicum activated is inoculated into the zymotic fluid that step (2) obtains with 10%v/v, 37 DEG C,
150rpm fermentation 132h, pH to 5.5 is adjusted every 12h.
In incubation, its butanol yield is measured by sampling every 12h.When xylan concentration is 60g/L, co-cultivation obtains
Butanol concentration highest, reached 2.61g/L；When xylan concentration is 30g/L, the butanol concentration for co-culturing to obtain only has
1.53g/L；Butanol concentration when 90g/L xylan concentration is also slightly below 60g/L xylans, is 2.37g/L.
The different vaccination methods pair of 3 clostridium acetobutylicum Clostridium acetobutylicum ATCC of embodiment 824
The influence of butanol yield
Method is with embodiment 2, the difference is that the concentration of xylan is 60g/L in fermentation medium, step (3) has activated
Clostridium acetobutylicum nutrient solution is put into 4 DEG C of centrifuges, rotating speed 6000rpm, 10min, then outwells supernatant, by bacterium mud
Add in the zymotic fluid of step (2), wherein the volume for activating good clostridium acetobutylicum nutrient solution is identical with the volume of zymotic fluid；
Then 37 DEG C are continued to cultivate, rotating speed 150rpm, and pH to 5.5 is adjusted every 12h, and ferment 132h；
In incubation, sampled every 12h and determine its butanol yield.When the vaccination ways co-cultivation with embodiment 2,
Butanol yield only has 2.61g/L；When the vaccination ways co-cultivation with the present embodiment, butanol yield has reached 8.34g/L；Therefore adopt
It is more excellent with the vaccination ways of the present embodiment.
Influence of the different inoculation time of the clostridium acetobutylicum of embodiment 4 to final butanol yield
Method with embodiment 3, unlike 4 groups of experiments are set in step (2), fermentation time is respectively 36h, 60h,
84h, 108h.
In incubation, sampled every 12h and determine its butanol yield.60h has been cultivated when being pyrolyzed sugared high temperature anaerobic bacterium M5
When add clostridium acetobutylicum bacterium mud, butanol yield highest, reach 8.34g/L, it currently with xylan is sole carbon that this, which is also,
Obtained highest butanol yield is co-cultured during source, sees Fig. 1, Fig. 2.In this system, the zytase of bacterial strain M5 secretions is effectively
Xylan is degraded, and obtains about 18g/L xylose, hereafter, in co-culture system, zytase and xylobiose enzyme still have
Higher enzyme activity, can degradation of xylan be continually xylose, and clostridium acetobutylicum can utilize the xylose that degraded obtains
Produce butanol.Inoculation time is too early, and xylanase activity is relatively low, does not utilize the degraded of follow-up xylan, and is inoculated with too late, then can lead
Cause bacterial strain M5 incubation times long, zytase and xylobiose enzyme secretion amount are reduced, and the enzyme previously secreted out of is at 55 DEG C
Enzyme activity declines, and so as to cause the enzyme activity of enzyme system to decline, and the xylose that Partial digestion obtains also is utilized by bacterial strain M5, so as to reduce
The carbon flow of clostridium acetobutylicum, ultimately result in butanol yield reduction.
Claims (9)
1. a kind of existed using the method for being pyrolyzed sugared high temperature anaerobic bacterium and clostridium acetobutylicum co-cultivation fermenting and producing butanol, its feature
In comprising the following steps：
(1) the sugared high temperature anaerobic bacterium of pyrolysis of activation is inoculated into the fermentation medium containing xylan and fermented, sent out
Zymotic fluid；
(2) clostridium acetobutylicum of activation is inoculated into the zymotic fluid that step (1) obtains, fermenting and producing butanol.
2. according to the method for claim 1, it is characterised in that step (1) the sugared high temperature anaerobic bacterium Classification And Nomenclature of pyrolysis
To be pyrolyzed sugared high temperature anaerobic bacterium (Thermoanaerobacterium thermosaccharolyticum), bacterial strain number is M5,
China typical culture collection center is stored in, preservation date is on 2 27th, 2017, and preserving number is CCTCC NO:M
2017072；Step (2) the clostridium acetobutylicum Classification And Nomenclature is clostridium acetobutylicum (Clostridium
Acetobutylicum), bacterial strain number is ATCC 824.
3. according to the method for claim 1, it is characterised in that the enzyme activity of zytase reaches in step (1) described zymotic fluid
During to 0.3-0.8U/mL, the clostridium acetobutylicum of activation is inoculated into the zymotic fluid that step (1) obtains, fermenting and producing butanol.
4. according to the method for claim 3, it is characterised in that the enzyme activity of zytase reaches in step (1) described zymotic fluid
During to 0.5-0.6U/mL, the clostridium acetobutylicum of activation is inoculated into fermenting and producing butanol in the zymotic fluid that step (1) obtains.
5. according to the method for claim 1, it is characterised in that the sugared high temperature anaerobic bacterium of pyrolysis of step (1) described activation
Inoculum concentration is the 1-10% of fermentation medium volume.
6. according to the method for claim 1, it is characterised in that step (1) described fermentation condition is：Fermentation temperature 50-65
DEG C, fermentation time 36-72h, fermentation pH are 5.5-8.5, rotating speed 0-120rpm.
7. according to the method for claim 1, it is characterised in that the fermented and cultured basigamy containing xylan described in step (1)
Fang Wei：0.5-2.0g/L NaCl, 0.5-2.0g/L K2HPO4, 0.5-2.0g/L KH2PO4, 1.0-5.0g/L dusty yeasts, 0.2-
1.0g/L MgCl2·6H2O, 0.1-0.6g/L NH4Cl, 0.01-0.05g/L CaCl2·2H2O, 0.5-2.0g/L FeCl2·
4H2O, 0.1-0.5g/L KCl, xylan 30-90g/L, solvent are water, adjust pH to 6.0-6.5.
8. according to the method for claim 1, it is characterised in that the inoculation of the clostridium acetobutylicum of step (2) described activation
Mode is as follows：By the clostridium acetobutylicum medium centrifugal of activation, the hair that obtained precipitation will be centrifuged is inoculated into step (1) and obtains
In zymotic fluid, wherein the clostridium acetobutylicum nutrient solution activated is identical with the fermentating liquid volume that step (1) obtains.
9. according to the method for claim 1, it is characterised in that step (2) described fermentation condition is：Fermentation temperature 35-39
DEG C, fermentation time 60-168h, fermentation pH are 4.5-7.0, rotating speed 100-200rpm.
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| CN111073877A (en) * | 2019-05-20 | 2020-04-28 | 南京工业大学 | Xylanase with excellent temperature stability and pH tolerance and application thereof |
| CN111269869A (en) * | 2020-02-10 | 2020-06-12 | 南京工业大学 | Construction method of recombinant clostridium acetobutylicum and application of recombinant clostridium acetobutylicum in preparation of butanol through semi-fiber fermentation |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109536565A (en) * | 2018-12-20 | 2019-03-29 | 南京工业大学 | A method of succinic acid is produced using the sugared high temperature anaerobic bacterium of pyrolysis and Actinobacillus succinogenes mixed fungus fermentation |
| CN111073877A (en) * | 2019-05-20 | 2020-04-28 | 南京工业大学 | Xylanase with excellent temperature stability and pH tolerance and application thereof |
| CN111269869A (en) * | 2020-02-10 | 2020-06-12 | 南京工业大学 | Construction method of recombinant clostridium acetobutylicum and application of recombinant clostridium acetobutylicum in preparation of butanol through semi-fiber fermentation |
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