CN101525326A - Method for extracting anthocyanin - Google Patents
Method for extracting anthocyanin Download PDFInfo
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- CN101525326A CN101525326A CN200810017617A CN200810017617A CN101525326A CN 101525326 A CN101525326 A CN 101525326A CN 200810017617 A CN200810017617 A CN 200810017617A CN 200810017617 A CN200810017617 A CN 200810017617A CN 101525326 A CN101525326 A CN 101525326A
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- acid
- extracting
- cowberry
- anthocyanidin
- membrane
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- Separation Using Semi-Permeable Membranes (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for extracting anthocyanin from a plant. The method comprises the following steps: crushing the plant firstly, then extracting the plant, filtering the obtained material through inorganic film ultrafiltration equipment, and adsorbing an obtained film filtering liquid to saturate through resin until washing water shows colorless; adsorbing the macroporous absorbent resin by 30 to 50 percent ethanol or methanol, and collecting an effluent liquid; and concentrating and drying the effluent liquid to obtain the anthocyanin. The method has the advantages of easy operation, high content of the anthocyanin and low cost.
Description
Technical field
The present invention relates to a kind of method of from plant, extracting anthocyanidin.
Background technology
The extracting method of existing anthocyanidin is to adopt macroporous adsorbent resin and the common realization of two kinds of methods of ion exchange resin, and the notification number that its specific implementation step can be announced referring on August 4th, 2004 is the Chinese patent file of CN1517343A.Use the alcohol immersion cowberry and then extract anthocyanidin, but this method cost height, residual impurity is more, adopts ion exchange resin treatment, because ion exchange resin treatment is comparatively complicated, regenerative power is poor, so operating process is comparatively loaded down with trivial details.
Summary of the invention
The invention provides a kind of method of simple to operate, cost is low, purity is high extraction anthocyanidin.
Technical scheme of the present invention is as follows:
A kind of method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverize:
I) get cranberry, pulverize, fruit is squeezed the juice;
The centrifugation of ii) fruit being squeezed the juice obtains pomace and fruit juice respectively;
2] extract
I) under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, extract the gained pomace 2～3 times through soaking and stirring red-tape operati process temperature≤60 ℃, and each soaking and stirring 1.5～2h obtains extracting solution;
3] filter:
I) red-tape operati process temperature≤60 ℃,
Ii) with step 2] extracting solution and the step 1 that finally obtain] in step I i) fruit juice that obtains mixes the formation mixed solution;
Iii) with mixed solution behind high speed centrifugation, get supernatant liquor and carry out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtain membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
3] resin purification:
I) red-tape operati process temperature≤60 ℃;
Ii) with step 3] the membrane filtration liquid resin absorption that finally obtains is to saturated;
Iii) will be adsorbed to saturated resin water and clean, removal of impurities is colourless until water lotion;
Iv) resolve macroporous adsorbent resin, collect effluent liquid with 30～50% ethanol or methyl alcohol;
V) concentrate effluent liquid and dry, obtain anthocyanidin.
Above-mentioned steps 1] in acid in acid-aqueous solution be hydrochloric acid, sulfuric acid, citric acid or glacial acetic acid.
Above-mentioned steps 1] in acid in acid-aqueous solution be glacial acetic acid.
Above-mentioned steps 1] service temperature be 40 ℃, acid-concentration of aqueous solution is 10%.
Above-mentioned steps 2] in the membrane pore size of mineral membrane ultrafiltration apparatus be 50nm.
Above-mentioned steps 2] in the membrane pore size of mineral membrane ultrafiltration apparatus be 50nm.
The present invention has the following advantages:
1. easy handling.The equipment complete mechanicalization of using in whole technological process, operator are through can be on duty after training, and are therefore simple to operate.
2. anthocyanidin content height.The present invention adopts advanced mineral membrane ultrafiltration apparatus to carry out ultrafiltration, removes impurity such as a large amount of starch, protein, slilme, through the further separation and purification of macroporous adsorptive resins, can reach 50%, and quality product is improved greatly.
3. cost is low.Owing in leaching process, adopt acid extraction, avoid using alcohol, reduced pure consumption; And the use of ultra-filtration membrane, effectively remove macromolecular substance, the adsorptive capacity of macroporous adsorbent resin is increased, improved the yield of product, also reduce cost simultaneously.
Embodiment
A kind of method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverize:
I) get cranberry, pulverize, fruit is squeezed the juice;
The centrifugation of ii) fruit being squeezed the juice obtains pomace and fruit juice respectively;
2] extract
I) under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, extract the gained pomace 2～3 times through soaking and stirring red-tape operati process temperature≤60 ℃, and each soaking and stirring 1.5～2h obtains extracting solution; Acid in acid-aqueous solution is hydrochloric acid, sulfuric acid, citric acid or glacial acetic acid; 10% glacial acetic acid is best at 40 ℃,
3] filter:
I) red-tape operati process temperature≤60 ℃,
Ii) with step 2] extracting solution and the step 1 that finally obtain] in step I i) fruit juice that obtains mixes the formation mixed solution;
Iii) with mixed solution behind high speed centrifugation, get supernatant liquor and carry out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtain membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
3] resin purification:
I) red-tape operati process temperature≤60 ℃;
Ii) with step 3] the membrane filtration liquid that finally obtains with resin absorption to saturated;
Iii) will be adsorbed to saturated resin water and clean, removal of impurities is colourless until water lotion;
Iv) resolve macroporous adsorbent resin, collect effluent liquid with 30～50% ethanol or methyl alcohol;
V) concentrate effluent liquid and dry, obtain anthocyanidin.
Claims (6)
1. a method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverize:
I) get cranberry, pulverize, fruit is squeezed the juice;
The centrifugation of ii) fruit being squeezed the juice obtains pomace and fruit juice respectively;
2] extract
I) under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, extract the gained pomace 2～3 times through soaking and stirring red-tape operati process temperature≤60 ℃, and each soaking and stirring 1.5～2h obtains extracting solution;
3] filter:
I) red-tape operati process temperature≤60 ℃,
Ii) with step 2] extracting solution and the step 1 that finally obtain] in step I i) fruit juice that obtains mixes the formation mixed solution;
Iii) with mixed solution behind high speed centrifugation, get supernatant liquor and carry out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtain membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
3] resin purification:
I) red-tape operati process temperature≤60 ℃;
Ii) with step 3] the membrane filtration liquid that finally obtains with resin absorption to saturated;
Iii) will be adsorbed to saturated resin water and clean, removal of impurities is colourless until water lotion;
Iv) resolve macroporous adsorbent resin, collect effluent liquid with 30～50% ethanol or methyl alcohol;
V) concentrate effluent liquid and dry, obtain anthocyanidin.
2. a kind of method of extracting anthocyanidin from cowberry according to claim 1 is characterized in that: described step 1] in acid in acid-aqueous solution be hydrochloric acid, sulfuric acid, citric acid or glacial acetic acid.
3. a kind of method of extracting anthocyanidin from cowberry according to claim 2 is characterized in that: described step 1] in acid in acid-aqueous solution be glacial acetic acid.
4. according to claim 1 or 2 or 3 described a kind of methods of from cowberry, extracting anthocyanidin, it is characterized in that: described step 1] service temperature be 40 ℃, acid-concentration of aqueous solution is 10%.
5. according to claim 1 or 2 or 3 described a kind of methods of from cowberry, extracting anthocyanidin, it is characterized in that: described step 2] in the membrane pore size of mineral membrane ultrafiltration apparatus be 50nm.
6. a kind of method of extracting anthocyanidin from cowberry according to claim 4 is characterized in that: described step 2] in the membrane pore size of mineral membrane ultrafiltration apparatus be 50nm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810017617A CN101525326A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810017617A CN101525326A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101525326A true CN101525326A (en) | 2009-09-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810017617A Pending CN101525326A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin |
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| CN (1) | CN101525326A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101879380A (en) * | 2010-06-25 | 2010-11-10 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN101712673B (en) * | 2009-10-14 | 2011-12-07 | 国家林业局林产工业规划设计院 | Method for extracting proanthocyanidins from raspberry |
| CN102807545A (en) * | 2012-08-08 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN109930395A (en) * | 2019-04-09 | 2019-06-25 | 中原工学院 | A kind of microbial staining method of cellulose-based fiber |
| CN109944098A (en) * | 2019-04-09 | 2019-06-28 | 中原工学院 | A kind of microbial staining method of protein fiber |
-
2008
- 2008-03-06 CN CN200810017617A patent/CN101525326A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101712673B (en) * | 2009-10-14 | 2011-12-07 | 国家林业局林产工业规划设计院 | Method for extracting proanthocyanidins from raspberry |
| CN101879380A (en) * | 2010-06-25 | 2010-11-10 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN101879380B (en) * | 2010-06-25 | 2012-07-18 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN102807545A (en) * | 2012-08-08 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN102807545B (en) * | 2012-08-08 | 2014-08-13 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN109930395A (en) * | 2019-04-09 | 2019-06-25 | 中原工学院 | A kind of microbial staining method of cellulose-based fiber |
| CN109944098A (en) * | 2019-04-09 | 2019-06-28 | 中原工学院 | A kind of microbial staining method of protein fiber |
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Open date: 20090909 |