CN101525325A - Method for extracting anthocyanin from cowberry - Google Patents
Method for extracting anthocyanin from cowberry Download PDFInfo
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- CN101525325A CN101525325A CN200810017611A CN200810017611A CN101525325A CN 101525325 A CN101525325 A CN 101525325A CN 200810017611 A CN200810017611 A CN 200810017611A CN 200810017611 A CN200810017611 A CN 200810017611A CN 101525325 A CN101525325 A CN 101525325A
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Abstract
The invention relates to a method for extracting anthocyanin from a cowberry. The method comprises the following steps: taking cowberry fruits, crushing the cowberry fruits, and squeezing the fruits into juice; centrifugating the juice to obtain fruit residues and fruit juice respectively; controlling the temperature of the operation process to less than or equal to 60 DEG C; soaking the obtained fruit residues in a 1 to 10 percent acid-water solution at an operation temperature of between 30 and 50 DEG C; soaking, stirring and extracting the fruit residues for 2 to 3 times, and soaking and stirring the fruit residues for 1.5 to 2 hours every time to obtain an extracting solution; mixing the extracting solution finally obtained and the fruit juice to form a mixed liquor, centrifugating the mixed liquor at a high speed, taking a supernate to carry out ultrafiltration by inorganic film ultrafiltration equipment to obtain a film filtering liquid; purifying resin; controlling the temperature of the operation process to less than or equal to 60 DEG C; adsorbing the film filtering liquid finally obtained to saturate by the resin; washing the resin adsorbed to saturate by water; removing impurities from the resin until washing water shows colorless; desorbing the macroporous absorbent resin by 30 to 50 percent ethanol or methanol, and collecting an effluent liquid; and concentrating and drying the effluent liquid to obtain the anthocyanin.
Description
Technical field
The present invention relates to a kind of method of from plant, extracting anthocyanidin, relate in particular to a kind of method of from cowberry, extracting anthocyanidin.
Background technology
The existing method of extracting anthocyanidin from cowberry is to adopt macroporous adsorbent resin and the common realization of two kinds of methods of ion exchange resin, use the alcohol immersion cowberry and then extract anthocyanidin, the notification number that its specific implementation step can be announced referring on August 4th, 2004 is the Chinese patent file of CN1517343A.This method has the cost height, and residual impurity is more, and adopts ion exchange resin treatment, because ion exchange resin treatment is comparatively complicated, regenerative power is poor, so the comparatively loaded down with trivial details shortcoming of operating process.
Summary of the invention
The invention provides the method for the extraction anthocyanidin that a kind of technology is simple, cost is low, anthocyanidin purity is high.
Technical scheme of the present invention is as follows:
A kind of method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverizes: get cranberry, pulverize, fruit is squeezed the juice; Centrifugation obtains pomace and fruit juice respectively;
2] extract: red-tape operati process temperature≤60 ℃, the gained pomace under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, is extracted 2～3 times through soaking and stirring, soaking and stirring 1.5～2h obtains extracting solution at every turn;
3] filter: red-tape operati process temperature≤60 ℃, the extracting solution and the step 1 that finally obtain] in the fruit juice that obtains mix, the formation mixed solution behind the high speed centrifugation, is got supernatant liquor and is carried out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtains membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
4] resin purification: red-tape operati process temperature≤60 ℃; With step 3] the membrane filtration liquid that finally obtains with resin absorption to saturated; Clean being adsorbed to saturated resin water, removal of impurities is colourless until water lotion; Ethanol with 30 ~ 50% or methyl alcohol are resolved macroporous adsorbent resin, collect effluent liquid; Concentrate effluent liquid and dry, obtain anthocyanidin.
The present invention has the following advantages:
1. easy handling.The equipment complete mechanicalization of using in whole technological process, operator are through can be on duty after training, and are therefore simple to operate.
2. anthocyanidin content height.The present invention adopts advanced mineral membrane ultrafiltration apparatus to carry out ultrafiltration, removes impurity such as a large amount of starch, protein, slilme, through the further separation and purification of macroporous adsorptive resins, can reach 50%, and quality product is improved greatly.
3. cost is low.Owing in leaching process, adopt acid extraction, avoid using alcohol, reduced pure consumption; And the use of ultra-filtration membrane, effectively remove macromolecular substance, the adsorptive capacity of macroporous adsorbent resin is increased, improved the yield of product, also reduce cost simultaneously.
Embodiment
A kind of method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverizes: get cranberry, pulverize, fruit is squeezed the juice; Centrifugation obtains pomace and fruit juice respectively;
2] extract: red-tape operati process temperature≤60 ℃, the gained pomace under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, is extracted 2～3 times through soaking and stirring, soaking and stirring 1.5～2h obtains extracting solution at every turn;
3] filter: red-tape operati process temperature≤60 ℃, the extracting solution and the step 1 that finally obtain] in the fruit juice that obtains mix, the formation mixed solution behind the high speed centrifugation, is got supernatant liquor and is carried out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtains membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
4] resin purification: red-tape operati process temperature≤60 ℃; With step 3] the membrane filtration liquid that finally obtains with resin absorption to saturated; Clean being adsorbed to saturated resin water, removal of impurities is colourless until water lotion; Ethanol with 30～50% or methyl alcohol are resolved macroporous adsorbent resin, and macroporous adsorbent resin is D101, AB-8, LSA-10, HPD-400, HP-20, ADS-17 or XAD-2.Collect effluent liquid; Concentrate effluent liquid and dry, obtain anthocyanidin.
Claims (7)
1. a method of extracting anthocyanidin from cowberry is characterized in that, comprises following steps:
1] pulverizes: get cranberry, pulverize, fruit is squeezed the juice; Centrifugation obtains pomace and fruit juice respectively;
2] extract: red-tape operati process temperature≤60 ℃, the gained pomace under 30～50 ℃ service temperature, with 1～10% acid-aqueous solution soaking, is extracted 2～3 times through soaking and stirring, soaking and stirring 1.5～2h obtains extracting solution at every turn;
3] filter: red-tape operati process temperature≤60 ℃, the extracting solution and the step 1 that finally obtain] in the fruit juice that obtains mix, the formation mixed solution behind the high speed centrifugation, is got supernatant liquor and is carried out ultrafiltration with the mineral membrane ultrafiltration apparatus, obtains membrane filtration liquid; Wherein the membrane pore size of mineral membrane ultrafiltration apparatus is 10nm, 20nm, 50nm or 100nm;
4] resin purification: red-tape operati process temperature≤60 ℃; With step 3] the membrane filtration liquid that finally obtains with resin absorption to saturated; Clean being adsorbed to saturated resin water, removal of impurities is colourless until water lotion; Ethanol with 3～50% or methyl alcohol are resolved macroporous adsorbent resin, collect effluent liquid; Concentrate effluent liquid and dry, obtain anthocyanidin.
2. a kind of method of extracting anthocyanidin from cowberry according to claim 1 is characterized in that: described step 1] in acid in acid-aqueous solution be hydrochloric acid, sulfuric acid, citric acid or glacial acetic acid.
3. a kind of method of extracting anthocyanidin from cowberry according to claim 2 is characterized in that: described step 1] in acid in acid-aqueous solution be glacial acetic acid.
4. according to claim 1 or 2 or 3 described a kind of methods of from cowberry, extracting anthocyanidin, it is characterized in that: described step 2] in the membrane pore size of mineral membrane ultrafiltration apparatus be 50nm.
5. according to claim 1 or 2 or 3 described a kind of methods of extracting anthocyanidin from cowberry, it is characterized in that: described macroporous adsorbent resin is D101, AB-8, LSA-10, HPD-400, HP-20, ADS-17 or XAD-2.
6. a kind of method of extracting anthocyanidin from cowberry according to claim 4, it is characterized in that: described macroporous adsorbent resin is D101, AB-8, LSA-10, HPD-400, HP-20, ADS-17 or XAD-2.
7. a kind of method of extracting anthocyanidin from cowberry according to claim 6, it is characterized in that: described macroporous adsorbent resin is D101, AB-8, LSA-10, HPD-400, HP-20, ADS-17 or XAD-2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810017611A CN101525325A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin from cowberry |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810017611A CN101525325A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin from cowberry |
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| CN101525325A true CN101525325A (en) | 2009-09-09 |
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| CN200810017611A Pending CN101525325A (en) | 2008-03-06 | 2008-03-06 | Method for extracting anthocyanin from cowberry |
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Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102731462A (en) * | 2011-12-25 | 2012-10-17 | 大兴安岭林格贝有机食品有限责任公司 | Method for purifying anthocyanosides in blueberry |
| CN102742701A (en) * | 2012-07-17 | 2012-10-24 | 北京绿源求证科技发展有限责任公司 | Edible eye caring tea granule for preventing asthenopia |
| CN102786506A (en) * | 2011-05-19 | 2012-11-21 | 长沙康隆生物制品有限公司 | Technology for rapidly preparing 25% anthocyanidins from cowberry |
| CN102783686A (en) * | 2012-07-04 | 2012-11-21 | 贵州省生物研究所 | Method for preparing blueberry juice beverage with high anthocyanin content |
| CN102807545A (en) * | 2012-08-08 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN103013936A (en) * | 2012-12-25 | 2013-04-03 | 浙江惠松制药有限公司 | Method for extracting anthocyanidin by using compound enzyme, and compound enzyme preparation thereof |
| CN103130762A (en) * | 2013-01-31 | 2013-06-05 | 宁波中药制药有限公司 | Method for extracting anthocyanin from indigo indicum |
| CN103130763A (en) * | 2013-02-26 | 2013-06-05 | 海南达川食品有限公司 | Method for preparing anthocyanin by using red-meat pitaya |
| CN103626733A (en) * | 2013-11-08 | 2014-03-12 | 天津大学 | Separation method of blueberry anthocyanin |
| CN103664856A (en) * | 2013-11-25 | 2014-03-26 | 宁波绿之健药业有限公司 | Cranberry extraction method |
| CN103665922A (en) * | 2012-08-31 | 2014-03-26 | 重庆还少堂生物技术有限责任公司 | Production process of blueberry extract with more than or equal to 25% of anthocyanin |
| CN104000260A (en) * | 2014-06-23 | 2014-08-27 | 陈智辉 | Method for preparing sea-buckthorn jaboticaba fruit vinegar carbonated beverage |
| CN104098633A (en) * | 2014-08-04 | 2014-10-15 | 湖南华诚生物资源有限公司 | Method for extracting anthocyanin from huckleberry |
| CN105294633A (en) * | 2015-11-13 | 2016-02-03 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing vaccinium vitis idaea anthocyanidin from vaccinium vitis idaea |
| CN106214731A (en) * | 2016-08-29 | 2016-12-14 | 盛林蓝莓集团股份有限公司 | Blueberry raw anthocyanidin biological extraction process |
| CN107440117A (en) * | 2017-08-04 | 2017-12-08 | 浙江绍兴东灵保健食品有限公司 | A kind of Bilberry fruit P.E production technology |
| CN109930395A (en) * | 2019-04-09 | 2019-06-25 | 中原工学院 | A kind of microbial staining method of cellulose-based fiber |
| CN110437197A (en) * | 2019-07-31 | 2019-11-12 | 湖南华诚生物资源股份有限公司 | A method of extracting anthocyanidin from butterfly Tofu pudding |
| CN114031952A (en) * | 2022-01-10 | 2022-02-11 | 罗麦(北京)营养食品研究有限公司 | Method for extracting anthocyanin from plant |
-
2008
- 2008-03-06 CN CN200810017611A patent/CN101525325A/en active Pending
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102786506A (en) * | 2011-05-19 | 2012-11-21 | 长沙康隆生物制品有限公司 | Technology for rapidly preparing 25% anthocyanidins from cowberry |
| CN102731462A (en) * | 2011-12-25 | 2012-10-17 | 大兴安岭林格贝有机食品有限责任公司 | Method for purifying anthocyanosides in blueberry |
| CN102731462B (en) * | 2011-12-25 | 2015-02-11 | 大兴安岭林格贝有机食品有限责任公司 | Method for purifying anthocyanosides in blueberry |
| CN102783686A (en) * | 2012-07-04 | 2012-11-21 | 贵州省生物研究所 | Method for preparing blueberry juice beverage with high anthocyanin content |
| CN102742701A (en) * | 2012-07-17 | 2012-10-24 | 北京绿源求证科技发展有限责任公司 | Edible eye caring tea granule for preventing asthenopia |
| CN102807545A (en) * | 2012-08-08 | 2012-12-05 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN102807545B (en) * | 2012-08-08 | 2014-08-13 | 宁波杰顺生物科技有限公司 | Method for preparing procyanidine extracts in cranberries |
| CN103665922A (en) * | 2012-08-31 | 2014-03-26 | 重庆还少堂生物技术有限责任公司 | Production process of blueberry extract with more than or equal to 25% of anthocyanin |
| CN103013936B (en) * | 2012-12-25 | 2014-12-03 | 浙江惠松制药有限公司 | Method for extracting anthocyanidin by using compound enzyme, and compound enzyme preparation thereof |
| CN103013936A (en) * | 2012-12-25 | 2013-04-03 | 浙江惠松制药有限公司 | Method for extracting anthocyanidin by using compound enzyme, and compound enzyme preparation thereof |
| CN103130762A (en) * | 2013-01-31 | 2013-06-05 | 宁波中药制药有限公司 | Method for extracting anthocyanin from indigo indicum |
| CN103130762B (en) * | 2013-01-31 | 2016-08-03 | 宁波中药制药股份有限公司 | A kind of method extracting anthocyanidin from indigo fruit |
| CN103130763A (en) * | 2013-02-26 | 2013-06-05 | 海南达川食品有限公司 | Method for preparing anthocyanin by using red-meat pitaya |
| CN103626733B (en) * | 2013-11-08 | 2015-07-29 | 天津大学 | Separation method of blueberry anthocyanin |
| CN103626733A (en) * | 2013-11-08 | 2014-03-12 | 天津大学 | Separation method of blueberry anthocyanin |
| CN103664856A (en) * | 2013-11-25 | 2014-03-26 | 宁波绿之健药业有限公司 | Cranberry extraction method |
| CN103664856B (en) * | 2013-11-25 | 2018-11-06 | 宁波绿之健药业有限公司 | A kind of extracting method of mossberry |
| CN104000260A (en) * | 2014-06-23 | 2014-08-27 | 陈智辉 | Method for preparing sea-buckthorn jaboticaba fruit vinegar carbonated beverage |
| CN104098633B (en) * | 2014-08-04 | 2017-01-11 | 湖南华诚生物资源股份有限公司 | Method for extracting anthocyanin from huckleberry |
| CN104098633A (en) * | 2014-08-04 | 2014-10-15 | 湖南华诚生物资源有限公司 | Method for extracting anthocyanin from huckleberry |
| CN105294633B (en) * | 2015-11-13 | 2018-08-28 | 大兴安岭林格贝寒带生物科技股份有限公司 | A kind of industrial method preparing cowberry anthocyanidin from cowberry |
| CN105294633A (en) * | 2015-11-13 | 2016-02-03 | 大兴安岭林格贝寒带生物科技股份有限公司 | Industrial method for preparing vaccinium vitis idaea anthocyanidin from vaccinium vitis idaea |
| CN106214731A (en) * | 2016-08-29 | 2016-12-14 | 盛林蓝莓集团股份有限公司 | Blueberry raw anthocyanidin biological extraction process |
| CN107440117A (en) * | 2017-08-04 | 2017-12-08 | 浙江绍兴东灵保健食品有限公司 | A kind of Bilberry fruit P.E production technology |
| CN109930395A (en) * | 2019-04-09 | 2019-06-25 | 中原工学院 | A kind of microbial staining method of cellulose-based fiber |
| CN110437197A (en) * | 2019-07-31 | 2019-11-12 | 湖南华诚生物资源股份有限公司 | A method of extracting anthocyanidin from butterfly Tofu pudding |
| CN110437197B (en) * | 2019-07-31 | 2021-04-13 | 湖南华诚生物资源股份有限公司 | Method for extracting anthocyanin from sphenoidea |
| CN114031952A (en) * | 2022-01-10 | 2022-02-11 | 罗麦(北京)营养食品研究有限公司 | Method for extracting anthocyanin from plant |
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Open date: 20090909 |