CN101492206A - Method for in situ restoring groundwater azotate pollution with corn stalk - Google Patents
Method for in situ restoring groundwater azotate pollution with corn stalk Download PDFInfo
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Abstract
The invention relates to a method for remedying the nitrate pollution of underground water in situ with maize straws, which is characterized in that maize straws or a maize straw hydrolysate serving as a denitrification carbon source and flora-enriched liquid rich in denitrifying bacteria florae are added in nitrate polluted underground water to remove nitrate nitrogen from underground water through bio-denitrification. The method realizes the harmless use of biomass maize straws and remedies the nitrate pollution of underground water effectively.
Description
Technical field
The present invention relates to a kind of restorative procedure of pollutent, being particularly related to the phreatic in-situ remediation method of a kind of azotate pollution, exactly is a kind ofly to utilize maize straw or/and maize straw hydrolyzed solution and denitrifying bacterium flora are removed and the method for prevention groundwater azotate pollution.
Background technology
The pollution of nitrate in groundwater was on the rise since the sixties in 20th century, owing to its stable in properties, solubleness height, moved with underground water transport, became many areas on the our times (especially lake drainage) the most general environmental factor of groundwater pollution.The excessive use of agrochemical, industrial sewage quantity discharged increase severely and the farmland big area is dirty irritates, and causes the azotate pollution in the underground water serious.
A large amount of nitrate in the underground water are harmful to human and animals and plants health, and the nitrate overwhelming majority that human body is taken in is from vegetables and tap water, higher concentration NO
3 -Picked-up can have a strong impact on human health, as baby's methemoglobin, blue baby syndromes, even carcinogenic.
The control groundwater azotate pollution is pressing issues, and biological denitrification is because of NO
3 -Can be changed into NO by denitrifying bacterium
2 -, NO, N
2O is until N
2, and the refuse that produces biosolids just usually is simple because of it, selectivity good and economical and effective has more environment and advantage economically than other method.
Denitrifying bacterium is the microorganism that carries out denitrification, can separate from physical environment.Most of heterotrophic denitrifying bacteriums need provide the organic carbon of capacity can finish biological denitrification, and the common organic carbon that lacks in the underground water, this just need provide additional carbon, the carbon source of using has the liquid carbon source at present, comprise methyl alcohol, ethanol, glucose, sucrose, acetate etc., solid carbon source is also arranged, as wheat straw, straw, cotton, newspaper and bark etc.In the face of what need to administer almost is endless azotate pollution underground water, and the liquid carbon source that adds produces secondary pollution because of excessive easily, and expense almost is a bottomless pit; And solid carbon source lacks flowability, and its main component is to be difficult to can not big area be repaired groundwater azotate pollution by direct Mierocrystalline cellulose, hemicellulose and the xylogen etc. that utilize of denitrifying bacterium.
Seriously regional at groundwater azotate pollution, the effluent seepage of refuse landfill, excessive use chemical fertilizer and farmland are dirty irritates, and its azotate pollution comprises by the face of land and infiltrating because of lithic drainage, comprises flowing into and outflow; The industry nitric wastewater discharges along irrigation canals and ditches, and its nitrate also comprises flowing into and outflow because of surface water.Inflow is meant the water that comes of the IA of will flowing through; Outflow is meant the water outlet through IA, and water outlet is exactly the underground water of azotate pollution.
Corn is main farm crop in autumn at northern area, and tradition is to adopt open incineration to handle maize straw, and this method pollutant atmosphere because of burning has destroyed the soil microorganisms flora in farmland, also is the waste of biomass energy simultaneously.
Summary of the invention
The present invention is for avoiding above-mentioned existing in prior technology weak point, provide a kind of economical and practical, effective maize straw original position of utilizing to repair the method for groundwater azotate pollution, make the biomass maize straw obtain innoxious utilization on the one hand, effectively eliminate groundwater azotate pollution on the other hand.
Technical solution problem of the present invention adopts following technical scheme:
The characteristics that the present invention utilizes the maize straw original position to repair the method for groundwater azotate pollution are in the underground water of azotate pollution, with maize straw or/and the maize straw hydrolyzed solution as denitrifying carbon source, add the enriching floras liquid that is rich in the denitrifying bacterium flora, by the nitric nitrogen in the biological denitrification removal underground water.
The characteristics of the inventive method also are:
Come water or/and on the path of water outlet, described is that cornstalk biological denitrification wall is set with maize straw as carbon source the phreatic of described azotate pollution.
Described maize straw hydrolyzed solution is to utilize cellulase, will be hydrolyzed into glucose or/and other monose through pretreated maize straw; The pretreatment process of described maize straw is steam explosion, wet oxidation, or with diluted acid, diluted alkaline, the ammoniacal liquor Chemical Pretreatment method to maize straw.
The addition means of described maize straw hydrolyzed solution and enriching floras liquid is in groundwater azotate pollution zone drilling well or hole, to pour into maize straw hydrolyzed solution and enriching floras liquid in described well or hole; Perhaps extract the underground water of azotate pollution out, with described maize straw hydrolyzed solution and enriching floras liquid with irritate back again after the underground water of extraction mixes.
Come water or/and on the path of water outlet described azotate pollution phreatic, be provided with respectively or biochemical treatment tank is set simultaneously, described biological denitrification carries out in biochemical treatment tank.
Shallow ground water and surface water come water or/and excavate ditch on the path of water outlet, described biological denitrification carries out in ditch.
When adding enriching floras liquid first, keep that denitrifying bacterium flora concentration is 10 in the water
2～10
5Individual/ml, the maize straw hydrolyzed solution of interpolation is 1～5 in the mol ratio C/N of nitrate nitrogen content in glucose carbon content and the water, and the pH value of adjusting the maize straw hydrolyzed solution simultaneously is 7.2～7.5.
Enriching floras liquid is the wetland soil that is rich in the denitrifying bacterium flora that comes from the physical environment, the mud on river, river, lake, ditch, the pool, the percolate of refuse landfill, mud, or one or more compounding substances in the sewage of sewage work, mud.
Compared with the prior art, beneficial effect of the present invention is embodied in:
1, the present invention utilizes the goal of the invention that maize straw provides carbon source and then realization original position to repair groundwater azotate pollution for the denitrifying bacterium flora from physical environment, add carbon source and denitrifying bacterium flora and nitrate nitrogen content in the water can be reduced to below the 10mg/L from about 50mg/L about one week, COD, nitrite nitrogen, colourity and the denitrification flora quantity in the water all can reach the groundwater quality standard simultaneously.
2, the inventive method is employed is wide material sources and cheap maize straw, make Mierocrystalline cellulose, hemicellulose and the xylogen of maize straw be hydrolyzed to the nutritive substance that bacterium is easy to utilize by the method for bio-transformation, not only avoid the problem of environmental pollution of open incineration stalk, also solved the problem of complex utilization of biomass energy.
3, the present invention adopts the pregnant solution of the denitrifying bacterium flora that contains in the anaerobic environment materials such as sewage, mud of percolate, mud, the sewage work of the mud be selected from wetland mud, river, river, lake, ditch, the pool, refuse landfill as the microorganism of adding, these are microbe-derived in physical environment, after the carbon source of being added runs out of, flora becomes feeble and die thereupon, is beneficial to the microbial ecological balance that keeps environment.
4, the present invention can adopt dischargings such as the waste water that is rich in organic carbon, nitrogen, phosphorus such as food factory, confectionery, Gourmet Powder Factory, Milk Products Plant, pulp mill the waste water preparation as the denitrifying bacterium enrichment medium, also solved the wastewater problem in these fields.
Description of drawings
Fig. 1 is the inventive method situation that cornstalk biological denitrification wall is repaired azotate pollution under different flow velocities.
Fig. 2 be in the inventive method the nitrated wall of cornstalk biological to the influence of nitrite content in the water.
Below by specific embodiment, the invention will be further described:
Embodiment
Concrete enforcement of the present invention is in the underground water of azotate pollution, or/and the maize straw hydrolyzed solution, adds the enriching floras liquid be rich in the denitrifying bacterium flora as denitrifying carbon source, removes nitric nitrogen in underground water by biological denitrification with maize straw.
In concrete the enforcement, is to come water or/and on the path of water outlet the phreatic of azotate pollution with maize straw as carbon source, construct permeable biological denitrification wall with maize straw, be that maize straw is cut into length is fragment below the 5cm, it mixes with the waterbearing stratum sand, adds cellulase simultaneously, and with the enriching floras liquid mixing that is rich in the denitrifying bacterium flora, make cornstalk biological reaction wall, this wherein:
The maize straw fragment is 2～4: 6～8 with the waterbearing stratum sand materials that mix by weight;
The consumption of the cellulase of the required interpolation of every gram dry straw is 25-30U;
Inoculum size is 1%-3%, promptly every hectogram dry straw inoculation denitrification flora pregnant solution 1-3ml, and the enrichment bacterial concentration is 10
4～10
7Between the order of magnitude.
The maize straw hydrolyzed solution is that technology is utilized cellulase routinely, will be hydrolyzed into glucose or/and other monose through pretreated maize straw, and every gram stalk needs the 25-30U cellulase in the hydrolytic process.
In maize straw, contain more poly-dextrose and polyxylose-pectinose-glucuronic acid, but be wrapped among hemicellulose and the xylogen, its crystalline structure is difficult to be degraded, enzyme and acid have been hindered to cellulosic effect, therefore must carry out pre-treatment to the maize straw raw material, the pretreated method of maize straw can be steam explosion, wet oxidation, or with diluted acid, diluted alkaline, the ammoniacal liquor Chemical Pretreatment method to maize straw.After steam explosion or wet oxidation or pre-treatment such as diluted acid, diluted alkaline or ammoniacal liquor with chemical method, xylogen is to cellulosic parcel in the releasing maize straw, thereby Mierocrystalline cellulose is come out, favourable enzymic hydrolysis, obtain more fermentable sugar, be glucose or/and other monose directly utilizes for microorganism easily.Stalk hydrolyzed solution good fluidity can go deep into Groundwater Flow, and the denitrification scope is wide, the effect that the reparation of groundwater azotate pollution original position is obtained.
At different environment, the addition means of maize straw hydrolyzed solution and enriching floras liquid can be for following several:
1, in groundwater azotate pollution zone drilling well or hole, perfusion maize straw hydrolyzed solution and enriching floras liquid in well or hole; Perhaps extract the underground water of azotate pollution out, with maize straw hydrolyzed solution and enriching floras liquid with irritate back again after the underground water of extraction mixes.
2, come water or/and on the path of water outlet azotate pollution phreatic, be provided with respectively or biochemical treatment tank is set simultaneously, biological denitrification carries out in biochemical treatment tank, and this biochemical treatment tank is equivalent to make up an artificial swamp.
3, shallow ground water and surface water come water or/and excavate ditch on the path of water outlet, biological denitrification carries out in ditch.
In above-mentioned three kinds of addition manners, when adding enriching floras liquid first, should keep that denitrifying bacterium flora concentration is 10 in the water
2～10
5Individual/ml, the maize straw hydrolyzed solution of interpolation is 1～5 in the mol ratio C/N of nitrate nitrogen content in glucose carbon content and the water, and the pH value of adjusting the maize straw hydrolyzed solution simultaneously is 7.2～7.5, makes the pH of the denitrification system of water body be in 6.5-7.5.In operational process,, promptly below the 10mg/L, thereby determine the consumption of best flora concentration and corn hydrolyzed solution so that the nitrate nitrogen content that flows out at last in the geographic underground water of azotate pollution is reduced to IDWS.
In concrete the enforcement, enriching floras liquid is taken from the wetland soil that is rich in the denitrifying bacterium flora in the physical environment, the mud on river, river, lake, ditch, the pool, the percolate of refuse landfill, mud, or one or more compounding substances in the sewage of sewage work, mud.
Denitrifying bacterium is the heterotrophism anaerobic, as all containing heterotrophic denitrifying bacterium in the anaerobic environment materials such as the sewage of the percolate of the mud on wetland mud, river, river, lake, ditch, the pool, refuse landfill, mud, sewage work, mud.Under the anaerobic condition with NO
x-N is an electron acceptor(EA), is electron donor with the organic carbon, and denitrifying bacterium utilizes the carbon source of maize straw as biological denitrification, under anaerobic or anoxia condition, utilizes the final receptor of nitrate as respiratory chain, and it is reduced into nitrous acid, NO, N
2O is until N
2Process, be called nitrate respiration or denitrification.NO in this process
3 -Or NO
2 -Replace oxygen as terminal electron acceptor, and produce ATP.When electronics when donor is transferred to acceptor, microorganism obtains energy, is used for synthetic new cellular material and keeps the vital movement of existing cell.
The denitrification course is: NO
3 -→ NO
2 -→ NO → N
2O → N
2
NO
3 -+ 5[H] (organic electronic donor) → 1/2N
2+ 2H
2O+OH
-
NO
2 -+ 3[H] (organic electronic donor) → 1/2N
2+ 2H
2O+OH
-
Annotate: [H] anyly can provide electronics, and can reductive NO
3 -, NO
2 -Be N
2Material, comprise organism, sulfide, H
+Deng.
Denitrification finally is reduced into NO, N with nitrate nitrogen
2O and N
2Remove from water, wherein the nitrous acid breathing is the center of denitrogenation, can increase, subtract other reaction before and after it.
In concrete the enforcement, utilize electromagnetic survey (Electro Magnetic is called for short EM) etc., can clearly verify the distribution and the trend of the geographic lithic drainage of azotate pollution of flowing through, i.e. the distribution of water and water outlet, trend.
The nutrient solution of enrichment of bacterial can also be with the waste water preparation of being rich in organic carbon, nitrogen, phosphorus, as the waste water preparation of dischargings such as food factory, confectionery, Gourmet Powder Factory, Milk Products Plant, pulp mill except the public chemical reagent prescription that is rich in carbon, nitrogen, phosphorus.
Embodiment:
Serious farmland district is an example with azotate pollution, and there are the face of land, lithic drainage in this farmland district, and upland water has the sanitary sewage from the Residential areas, and a plurality of surface flow inlets are arranged; The irrigation canals and ditches that there is industrial nitrogenous effluent discharging on the next door infiltrate the farmland by the face of land, because excessive use nitrogenous fertilizer is irritated with dirty, nitrate nitrogen content is higher than China three class water quality standard 20mg/L in this farmland local ground watering between 30-70mg/L; The downstream is the lake, and the farmland lithic drainage is by the groundwater feed lake.
Biology in situ is repaired underground water and is carried out according to following steps:
1, to the in-situ remediation method of farmland district lithic drainage azotate pollution:
A, (EMlectromagnetic Survey, EM) technology is carried out field survey, determines that this farmland district comes the distribution and the trend of water and water outlet with electromagnetic survey.And calculate this according to its scope (length and width, the degree of depth) and distinguish phreatic Total Water V.
B, on-the-spot water sampling, lab analysis come the concentration of nitrate nitrogen, nitrite nitrogen, COD, dissolved total content of organic carbon (TOC), colourity, denitrifying bacterium flora in water and the water outlet with active.Conventional chemistry and bioanalysis are adopted in test.
Mensuration nitrate nitrogen, COD, dissolved total content of organic carbon (TOC) are intended to calculate to determine to add how many maize straw hydrolyzed solutions according to C/N, for example calculation result need be added the dissolved total organic carbon of 1000kg as the denitrification carbon source, and dissolved organic carbon (with glucose meter) content is 6～8g/L in the corn hydrolyzed solution that cellulase hydrolysis obtains, and the corn hydrolyzed solution total amount that then should add is 1000*180/72/ (6～8)=312.5～416.7m
3The rest may be inferred by analogy for it.
Measure the concentration and the active pregnant solution that is intended to determine to add for the first time how many denitrifying bacterium floras of denitrifying bacterium flora, make denitrifying bacterium flora concentration satisfy 10
2～10
5Individual/ml.
C, the trend of learning according to electromagnetic survey in the farmland district of coming water and water outlet select 4～8 points to adopt conventional drilling or easier high pressure geology directly to hole.
Determine to add the efficient denitrifying bacterium pregnant solution of purifying or/and contain the amount of the surrounding material pregnant solution and the above-mentioned maize straw hydrolyzed solution of denitrifying bacterium flora according to the water yield and water sample analysis data, in hole (well), inject the efficient denitrifying bacterium pregnant solution of purifying or/and contain the surrounding material pregnant solution and the above-mentioned maize straw hydrolyzed solution of denitrifying bacterium flora with the pressurization injection method, make denitrifying bacterium concentration reach 10
2-10
5Individual/ml, C/N is between 1～5.Perhaps extract underground water out, mix the efficient denitrifying bacterium pregnant solution of purifying, make denitrifying bacterium concentration reach 10 or/and contain the surrounding material pregnant solution and the above-mentioned stalk hydrolyzed solution of denitrifying bacterium flora
2-10
5Individual/ml, C/N between 1～5 after again the pressurization irritate back in the hole (well).
Nitrate nitrogen after the random sampling analyzing and processing, nitrite nitrogen, COD value, colourity and pH value make every index reach national groundwater quality standard.
2, the shallow table lithic drainage reparation that causes that the irrigation canals and ditches sewage effluent is infiltrated the farmland:
A, (EMlectromagnetic Survey, EM) technology is carried out field survey, determines that this farmland district comes the distribution and the trend of water and water outlet with electromagnetic survey.And calculate this according to its scope (length and width, the degree of depth) and distinguish phreatic Total Water V.
B, on-the-spot water sampling, lab analysis come the concentration of nitrate nitrogen, nitrite nitrogen, COD, dissolved total content of organic carbon (TOC), colourity, denitrifying bacterium flora in water and the water outlet with active.Conventional chemistry and bioanalysis are adopted in test.
Mensuration nitrate nitrogen, COD, dissolved total content of organic carbon (TOC) are intended to calculate to determine to add how many maize straw hydrolyzed solutions according to C/N, for example calculation result need be added the dissolved total organic carbon of 1000kg as the denitrification carbon source, and dissolved organic carbon (with glucose meter) content is 6～8g/L in the corn hydrolyzed solution that cellulase hydrolysis obtains, and the corn hydrolyzed solution total amount that then should add is 1000*180/72/ (6～8)=312.5～416.7m
3The rest may be inferred by analogy for it.
Measure the concentration and the active pregnant solution that is intended to determine to add for the first time how many denitrifying bacterium floras of denitrifying bacterium flora, make denitrifying bacterium flora concentration satisfy 10
2～10
5Individual/ml.
C, the irrigation canals and ditches of industrial discharge nitrogenous effluent are infiltrated the farmland by the face of land, can come 20～30 meters of water to excavate ditch in distance, filling or/and enrichment contains the surrounding material and the above-mentioned maize straw hydrolyzed solution of denitrifying bacterium flora, makes denitrifying bacterium concentration reach 10 by the efficient denitrifying bacterium of purifying in ditch
2-10
5Individual/ml, C/N is between 1～5.
Nitrate nitrogen after the random sampling analyzing and processing, nitrite nitrogen, COD value, colourity and pH value make every index reach national groundwater quality standard.
3, to the lithic drainage reparation in the serious farmland of azotate pollution district
A, (EMlectromagnetic Survey, EM) technology is carried out field survey, determines that this farmland district comes the distribution and the trend of water and water outlet with electromagnetic survey.And calculate this according to its scope (length and width, the degree of depth) and distinguish phreatic Total Water V.
B, on-the-spot water sampling, lab analysis come the concentration of nitrate nitrogen, nitrite nitrogen, COD, dissolved total content of organic carbon (TOC), colourity, denitrifying bacterium flora in water and the water outlet with active.Conventional chemistry and bioanalysis are adopted in test.
Mensuration nitrate nitrogen, COD, dissolved total content of organic carbon (TOC) are intended to calculate to determine to add how many maize straw hydrolyzed solutions according to C/N, for example calculation result need be added the dissolved total organic carbon of 1000kg as the denitrification carbon source, and dissolved organic carbon (with glucose meter) content is 6～8g/L in the corn hydrolyzed solution that cellulase hydrolysis obtains, and the corn hydrolyzed solution total amount that then should add is 1000*180/72/ (6～8)=312.5～416.7m
3The rest may be inferred by analogy for it.
Measure the concentration and the active pregnant solution that is intended to determine to add for the first time how many denitrifying bacterium floras of denitrifying bacterium flora, make denitrifying bacterium flora concentration satisfy 10
2～10
5Individual/ml.
C, come water or/and on the path of water outlet in the serious farmland of groundwater azotate pollution district, be provided with or be provided with simultaneously maize straw reaction wall respectively, to wherein adding cellulase or inoculation cellulase producing bacteria, and add respectively or add simultaneously by the surrounding material pregnant solution that contains the denitrifying bacterium flora or/and the denitrifying bacterium pregnant solution of purifying makes denitrifying bacterium concentration reach 10
2-10
5Individual/ml, C/N is between 1～5; Or be provided with or be provided with simultaneously efficient denitrifying bacterium by purifying respectively or/and enrichment contains the surrounding material of denitrifying bacterium flora and the biochemical treatment tank that above-mentioned maize straw hydrolyzed solution is formed, make denitrifying bacterium concentration reach 10
2-10
5Individual/ml, C/N is between 1～5.
Nitrate nitrogen after the random sampling analyzing and processing, nitrite nitrogen, COD value, colourity and pH value make every index reach national groundwater quality standard.
Go out to be provided with on the water route experiment of maize straw reaction wall below by the serious farmland district of simulated groundwater azotate pollution:
1, the removal situation of nitric nitrogen in the water outlet, under different flow velocity situations, water inlet nitrate nitrogen content is 50mg/L, and after the maize straw reaction wall that the sand layer of process 60-70cm and 10cm are thick successively, the content of nitrate nitrogen over time as shown in Figure 1 in the water outlet.
General underground water flow velocity is tens centimetres of every days, and experimental result shows that the denitrifying bacterium flora concentration of interpolation is 10
2-10
5In the time of between individual/ml, the maize straw reaction wall that is provided with has only 10cm thickness, be about to through 120h that nitrate nitrogen content is reduced to below 10 by 50mg/L in the water, and the slow more removal effect of flow velocity is good more, prolongation denitrification meeting in time continues, and nitrate concentration also can further reduce in the water outlet.
2, the changing conditions of nitrite in the water outlet, behind the biological denitrification through maize straw reaction wall, nitrite nitrogen content with the variation of flow velocity as shown in Figure 2 in the water outlet.
As shown in Figure 2, denitrifying bacterium flora from physical environment can utilize maize straw to carry out biological denitrification as carbon source, begin for some time the accumulation that (24-48h) has nitrite nitrogen in the water outlet, carrying out along with denitrification, nitrite nitrogen is eliminated, nitrite nitrogen is eliminated substantially fully behind 120h, also can reach portable water quality standard when flow velocity higher (40cm/d), and the slow more then final outflow water of flow velocity water quality is good more.
3, the growing state of denitrifying bacterium flora in the water outlet, after maize straw reaction wall adds denitrifying bacterium enriching floras liquid, with Seignette salt denitrifying bacterium substratum, under the flow velocity of 20cm/d, adopt the gradient dilution Plating to measure denitrifying bacterium flora quantity in the water outlet, the result is as shown in table 1.
Denitrification flora quantity in the water outlet of table 1 reaction wall
By table 1 as seen, the 24-48 h growth after inoculation of denitrifying bacterium flora is rapid, and 48 h reach the stable of quantity, enter decline phase behind 96h, and quantity reduces rapidly.After carbon source runs out of, lack the nutritive substance of bacterial growth in the water, its quantity also can reduce significantly.Lithic drainage and soil have complicated microorganism ecological environment in addition, exist between the flora and vie each other, the growth cycle that adds the denitrifying bacterium flora is short, therefore as long as time expand, the denitrifying bacterium flora that is added can reduce to the nature level, can therefore not cause phreatic bacterial contamination.
4, other as COD, colourity and pH value by experiment detected result all can reach the groundwater quality standard.
Claims (8)
1, utilize the maize straw original position to repair the method for groundwater azotate pollution, it is characterized in that in the underground water of azotate pollution, with maize straw or/and the maize straw hydrolyzed solution as denitrifying carbon source, add the enriching floras liquid that is rich in the denitrifying bacterium flora, by the nitric nitrogen in the biological denitrification removal underground water.
2, method according to claim 1 is characterized in that coming water or/and on the path of water outlet, described is that cornstalk biological denitrification wall is set with maize straw as carbon source the phreatic of described azotate pollution.
3, method according to claim 1 is characterized in that described maize straw hydrolyzed solution is to utilize cellulase, will be hydrolyzed into glucose or/and other monose through pretreated maize straw; The pretreatment process of described maize straw is steam explosion, wet oxidation, or with diluted acid, diluted alkaline, the ammoniacal liquor Chemical Pretreatment method to maize straw.
4, method according to claim 1 is characterized in that the addition means of described maize straw hydrolyzed solution and enriching floras liquid is, in groundwater azotate pollution zone drilling well or hole, and perfusion maize straw hydrolyzed solution and enriching floras liquid in described well or hole; Perhaps extract the underground water of azotate pollution out, with described maize straw hydrolyzed solution and enriching floras liquid with irritate back again after the underground water of extraction mixes.
5, method according to claim 1 is characterized in that coming water or/and on the path of water outlet described azotate pollution phreatic, is provided with respectively or biochemical treatment tank is set simultaneously, and described biological denitrification carries out in biochemical treatment tank.
6, method according to claim 1, it is characterized in that shallow ground water and surface water come water or/and excavate ditch on the path of water outlet, described biological denitrification carries out in ditch.
7,, when it is characterized in that adding enriching floras liquid first, keep that denitrifying bacterium flora concentration is 10 in the water according to claim 4,5 or 6 described methods
2～10
5Individual/ml, the maize straw hydrolyzed solution of interpolation is 1～5 in the mol ratio C/N of nitrate nitrogen content in glucose carbon content and the water, and the pH value of adjusting the maize straw hydrolyzed solution simultaneously is 7.2～7.5.
8, method according to claim 1, it is characterized in that enriching floras liquid is the wetland soil that is rich in the denitrifying bacterium flora that comes from the physical environment, the mud on river, river, lake, ditch, the pool, the percolate of refuse landfill, mud, or one or more compounding substances in the sewage of sewage work, mud.
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2009
- 2009-03-09 CN CN2009101163147A patent/CN101492206B/en not_active Expired - Fee Related
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