CN101482553A - Measuring apparatus and method for adhesive force between biological living body and object - Google Patents
Measuring apparatus and method for adhesive force between biological living body and object Download PDFInfo
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- CN101482553A CN101482553A CNA2008102032355A CN200810203235A CN101482553A CN 101482553 A CN101482553 A CN 101482553A CN A2008102032355 A CNA2008102032355 A CN A2008102032355A CN 200810203235 A CN200810203235 A CN 200810203235A CN 101482553 A CN101482553 A CN 101482553A
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Abstract
The invention discloses a measurement device and method for adhesion between biological living-body and object, belonging to the mechanic measuring instrument field. The measurement device comprises a transparent box capable of being opened, an adhesion measurement induction piece, rotary loading platform, a pick-up head, an electrodynamic trace sample injector, a temperature sensor, a humidity sensor, a computer and a displaying and printing device. The adhesion between biological living-body and various rough surfaces can be measured by the measurement device and method.
Description
Technical field
The invention belongs to the mechanical measuring instrument field, it relates to a kind of device for measuring force and method of testing of measuring adhesion between biological living and object.
Background technology
Measuring technique is considered to scientific research all the time, produces the effective means that makes a breakthrough.So the development of measuring technique is played crucial effects to the progress in fields such as research and production.At present, adhesion numerical value obtains by probe atomic force microscope (AFM), mechanics sensor quasi-instrument often between the acquisition object.But the probe atomic force microscope can not be measuring object with the biological living, and the selection of tested object has significant limitation, and according to the difference of test purpose, often needs to change some structure and realize that cost is than higher; The sample preparation that the mechanics sensor quasi-instrument is measured the biological living mechanical property requires than higher, especially the higher sensor of precision is easy in use be damaged, simultaneously, above-mentioned means of testing all can't be observed the dynamic behaviour of biological living in whole dynamometry process.
Summary of the invention
At above-mentioned defective, the invention provides a kind of device for measuring force and method of testing of measuring adhesion between biological living and object is the problem that biological living, instrument damage easily to solve the measurement thing.
The technical scheme of technical solution problem of the present invention is as follows:
A kind of device for measuring force of measuring adhesion between biological living and object, it comprises camera 2, temperature sensor 5, humidity sensor 6, electrodynamic type microsyringe 7, store memory has the computing machine 8 of Flame Image Process and data processing software, display 9, printer 10, worktable 11, but it is characterized in that it also comprises by the transparent casing 1 of open type that is placed on the outer cover 101 on the worktable 11 and the dodge gate 102 on outer cover 101 antethecas of being connected through the hinge is formed, its both common rotatable objective tables 3 of forming of first jacking gear that the first lift adjustment nut 322 that has bracing frame that first lift adjustment screw rod 321 that is linked to each other with the first lift adjustment nut 322 that has bracing frame by the loading round platform 31 that has ball bearing that links together with the first lift adjustment screw rod, 321 upper ends and its middle part that linked to each other with the ball bearing of the loading round platform 31 that has ball bearing by the upper end and middle part link to each other with the first lift adjustment screw rod 321 constitutes, level meter 413 belows that the leveling primary nut 412 that is sleeved on two ends, antenna formula pull bar 41 left and right sides by the magnetic sheet 411 that is packed in antenna formula pull bar 41 left ends belows is packed in antenna formula pull bar 41 centers are packed in antenna formula pull bar 41 right-hand members and by the reservoir 414 that conduit links to each other with electrodynamic type microsyringe 7 is formed and the antenna formula pull bar 41 that is supported on the second lift adjustment screw rod, 421 upper ends by the middle and lower part and the second lift adjustment nut 422 that has bracing frame that the second lift adjustment screw rod, 421 middle parts that link to each other with the second lift adjustment nut 422 that has bracing frame in the middle part of the upper end links to each other with antenna formula pull bar 41 middle and lower parts link to each other with the second lift adjustment screw rod 421 constitute its both common dynamometry induction pieces 4 of forming of second jacking gear.
But the transparent casing 1 of described open type can be made by transparent organic glass or transparent high polymer plastics, its shape can be rectangular parallelepiped or cube, length L is that 40-60cm, width W are that 30-50cm, height H are 30-50cm, places dodge gate 102 length L on outer cover 101 antethecas ' be that 30-50cm, width H ' they are 20-40cm.
A kind of method of testing of measuring the device for measuring force of adhesion between biological living and object is characterized in that realizing as follows:
[1] opens computing machine 8 preheatings ten minutes;
[2] but open the dodge gate 102 of the transparent casing 1 of open type, the second lift adjustment screw rod 421 of turn dynamometry induction pieces 4 second jacking gears is with the height of the regulating antenna formula pull bar 41 low 5-10mm of height to the left end magnetic sheet 411 of the position ratio antenna formula pull bar 41 that makes camera 2; The first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn makes the aspect ratio antenna formula pull bar 41 low 8-10cm of the loading round platform 31 that has rolling bearing;
[3] according to the desired measuring accuracy of adhesion between biological sample (A) and object, find the required length of antenna formula pull bar 41 of precision correspondence therewith by table 1, its value of the arm of force of then measuring the centreline space of the center line of reservoir 414 of antenna formula lever 41 right-hand members and the second lift adjustment screw rod 421 is L
pWith its value of the arm of force of the center line of measuring left end magnetic sheet 411 and the centreline space of the second lift adjustment screw rod 421 be L
d, and with L
p, L
dValue input computing machine 8;
Table 1
| The measuring accuracy (mg) of adhesion between biological sample (A) and object | 0.02-0.1 | 0.2-1 | 1.1-5 |
| The length (cm) of antenna formula pull bar | 40 | 30 | 20 |
[4] regulate the leveling primary nut 412 at two ends, antenna formula pull bar 41 left and right sides, whether the bubble of observing the level meter 413 at antenna formula pull bar 41 centers regulates the initial balance of antenna formula pull bar 41 in the center;
[5] with biological sample A, as fly class or cricket or spider or beetle, one that gets certain claims that its quality is M
dAnd with its value input computing machine 8, then this biological sample A is fixed on the existing sample preparation magnetic sheet, make magnetic sheet 411 adhesives of this sample preparation magnetic sheet and antenna formula pull bar 41 left ends below, biosome sample A is suspended on the magnetic sheet 411, thereby antenna formula pull bar 41 left ends is laid particular stress on and slightly downward-sloping;
[6] with object B, as sand paper or polishing paper or glass or plastics or colloid, be placed on the loading round platform 31 that has rolling bearing of rotatable objective table 3, the loading round platform 31 that turn has rolling bearing moves under the magnetic sheet 411 object B, and the first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn slowly rises to the loading round platform 31 that has rolling bearing to make with biosome A and just contacts;
[7] but regulate temperature sensor 5, humidity sensor 6 and make that ambient temperature and humidity is controlled at temperature 20-25 ℃, humidity 65-70% in the transparent casing 1 of open type; But close the dodge gate 102 of the transparent casing 1 of open type then immediately;
[8] open the function software of the control electrodynamic type microsyringe 7 of computing machine 8, each sample size of electrodynamic type microsyringe 7 is set, each sample size is light weight liquid such as alcohol or low density polyethylene, each sample size p is 0.02mg-2g, mouse is every to be clicked, with the sample size injection reservoir 414 of this setting;
[9] utilize camera 2 to take the whole loading process of biological sample A and be presented on the display 9, in this whole shooting process, can preserve arbitrarily one moment picture, machine 8 image processing softwares print through printer 10 after handling as calculated;
[10] action of clicking the mouse in the repeating step [8] when observing the moment that biological sample A separates with object B on the loading round platform 31 that has rolling bearing on the display 9, stops to click the mouse;
[11] arm of force L of the centreline space of the center line of the reservoir 414 of antenna formula lever 41 right-hand members by having imported computing machine 8 in the step [3] and the second lift adjustment screw rod 421
p, the center line of left end magnetic sheet 411 and the second lift adjustment screw rod 421 the arm of force L of centreline space
dAnd imported M in the computing machine 8 in the step [5]
d, and each sample size p that sets and click sum N calculate the adhesion numerical value F that biological sample A and object B should moments by following formula by computing machine 8
a
In the formula, M
pBe the gross mass of light weight liquid, M
p=p * N, g are acceleration of gravity, and its value is 9.8N/kg;
[12] repeated test step [1]-[11] are tested 20 biosome A of the same race altogether, and 20 data of acquisition machine 8 data processing softwares are as calculated handled, and obtain the adhesion mean value, standard variance between this kind biosome A and object B and are printed by printer 10.
Advantage compared with prior art of the present invention is:
(1) the measuring accuracy difference required according to different biosomes adopted antenna formula Tiebar structure;
(2) eliminated the problem that Micro-force sensor damages easily in the dynamometry process;
(3) camera is housed and takes in real time, realize online observation, can observe the dynamic behaviour of biosome in whole mechanical meaurement;
(4) scope of measuring object is wide, can measure biological living, and cost is lower.
Description of drawings:
Fig. 1 is a kind of device for measuring force synoptic diagram of measuring adhesion between biological living and object
But Fig. 2 is the structural representation of the transparent casing of open type
Fig. 3 is the structural representation of rotatable objective table
Fig. 4 is the structural representation of dynamometry induction pieces
Fig. 1, Fig. 2, Fig. 3, among Fig. 4, but the transparent casing of 1-open type, the 2-camera, the rotatable objective table of 3-, 31-have the loading round platform of rolling bearing, the 321-first lift adjustment screw rod, 322-has the first lift adjustment nut of bracing frame, 4-dynamometry induction pieces, 41-antenna formula pull bar, 411-magnetic sheet, 412-leveling primary nut, the 413-level meter, 414-reservoir, the 421-second lift adjustment screw rod, 422-has the second lift adjustment nut of bracing frame, the 5-temperature sensor, 6-humidity sensor, 7-electrodynamic type microsyringe, the 8-store memory has the computing machine of Flame Image Process and data processing software, the 9-display, 10-printer, 11-worktable.
Embodiment:
A kind of device for measuring force of measuring adhesion between biological living and object, it comprises camera 2, temperature sensor 5, humidity sensor 6, electrodynamic type microsyringe 7, store memory has the computing machine 8 of Flame Image Process and data processing software, display 9, printer 10, worktable 11, it is characterized in that but it also comprises by the transparent casing 1 of open type that is placed on the outer cover 101 on the worktable 11 and the dodge gate 102 on outer cover 101 antethecas of being connected through the hinge is formed, its both common rotatable objective tables 3 of forming of first jacking gear that the first lift adjustment nut 322 that has bracing frame that first lift adjustment screw rod 321 that is linked to each other with the first lift adjustment nut 322 that has bracing frame by the loading round platform 31 that has ball bearing that links together with the first lift adjustment screw rod, 321 upper ends and its middle part that linked to each other with the ball bearing of the loading round platform 31 that has ball bearing by the upper end and middle part link to each other with the first lift adjustment screw rod 321 constitutes, level meter 413 belows that the leveling primary nut 412 that is sleeved on two ends, antenna formula pull bar 41 left and right sides by the magnetic sheet 411 that is packed in antenna formula pull bar 41 left ends belows is packed in antenna formula pull bar 41 centers are packed in antenna formula pull bar 41 right-hand members and by conduit (on the figure mark), the reservoir that links to each other with electrodynamic type microsyringe 7 414 is formed and is supported on the antenna formula pull bar 41 of the second lift adjustment screw rod, 421 upper ends by the middle and lower part, thus this place be designed to antenna formula purpose be can adjusting yoke length adapt to of the different requirements of different measured objects to measuring accuracy; Link to each other with antenna formula pull bar 41 middle and lower parts second jacking gear its both of the second lift adjustment nut, 422 formations that have bracing frame that the second lift adjustment screw rod, 421 middle parts that the middle part links to each other with the second lift adjustment nut 422 that has bracing frame link to each other with the second lift adjustment screw rod 421 of this antenna formula pull bar 41 and upper end is formed dynamometry induction pieces 4 jointly.
But the transparent casing 1 of described open type can be made by transparent organic glass or transparent high polymer plastics, its shape can be rectangular parallelepiped or cube, length L is that 40-60cm, width W are that 30-50cm, height H are 30-50cm, places dodge gate 102 length L on outer cover 101 antethecas ' be that 30-50cm, width H ' they are 20-40cm.
A kind of method of testing of measuring the device for measuring force of adhesion between biological living and object is characterized in that realizing as follows:
[1] opens computing machine 8 preheatings ten minutes;
[2] but open the dodge gate 102 of the transparent casing 1 of open type, the second lift adjustment screw rod 421 of turn dynamometry induction pieces 4 second jacking gears is with the height of the regulating antenna formula pull bar 41 low 5-10mm of height to the left end magnetic sheet 411 of the position ratio antenna formula pull bar 41 that makes camera 2; The first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn makes the aspect ratio antenna formula pull bar 41 low 8-10cm of the loading round platform 31 that has rolling bearing;
[3] according to the desired measuring accuracy of adhesion between biological sample (A) and object, find the required length of antenna formula pull bar 41 of precision correspondence therewith by table 1, its value of the arm of force of then measuring the centreline space of the center line of reservoir 414 of antenna formula lever 41 right-hand members and the second lift adjustment screw rod 421 is L
pWith its value of the arm of force of the center line of measuring left end magnetic sheet 411 and the centreline space of the second lift adjustment screw rod 421 be L
d, and with L
p, L
dValue input computing machine 8;
Table 1
| The measuring accuracy (mg) of adhesion between biological sample (A) and object | 0.02-0.1 | 0.2-1 | 1.1-5 |
| The length (cm) of antenna formula pull bar | 40 | 30 | 20 |
[4] regulate the leveling primary nut 412 at two ends, antenna formula pull bar 41 left and right sides, whether the bubble of observing the level meter 413 at antenna formula pull bar 41 centers regulates the initial balance of antenna formula pull bar 41 in the center;
[5] with biological sample A, as fly class or cricket or spider or beetle, one that gets certain claims that its quality is M
dAnd with its value input computing machine 8, then this biological sample A is fixed on the existing sample preparation magnetic sheet, make magnetic sheet 411 adhesives of this sample preparation magnetic sheet and antenna formula pull bar 41 left ends below, biosome sample A is suspended on the magnetic sheet 411, thereby antenna formula pull bar 41 left ends is laid particular stress on and slightly downward-sloping;
[6] with object B, as sand paper or polishing paper or glass or plastics or colloid, be placed on the loading round platform 31 that has rolling bearing of rotatable objective table 3, the loading round platform 31 that turn has rolling bearing moves under the magnetic sheet 411 object B, and the first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn slowly rises to the loading round platform 31 that has rolling bearing to make with biosome A and just contacts;
[7] but regulate temperature sensor 5, humidity sensor 6 and make that ambient temperature and humidity is controlled at temperature 20-25 ℃, humidity 65-70% in the transparent casing 1 of open type; But close the dodge gate 102 of the transparent casing 1 of open type then immediately;
[8] open the function software of the control electrodynamic type microsyringe 7 of computing machine 8, each sample size of electrodynamic type microsyringe 7 is set, each sample size is light weight liquid such as alcohol or low density polyethylene, each sample size p is 0.02mg-2g, mouse is every to be clicked, with the sample size injection reservoir 414 of this setting;
[9] utilize camera 2 to take the whole loading process of biological sample A and be presented on the display 9, in this whole shooting process, can preserve arbitrarily one moment picture, machine 8 image processing softwares print through printer 10 after handling as calculated;
[10] action of clicking the mouse in the repeating step [8] when observing the moment that biological sample A separates with object B on the loading round platform 31 that has rolling bearing on the display 9, stops to click the mouse;
[11] arm of force L of the centreline space of the center line of the reservoir 414 of antenna formula lever 41 right-hand members by having imported computing machine 8 in the step [3] and the second lift adjustment screw rod 421
p, the center line of left end magnetic sheet 411 and the second lift adjustment screw rod 421 the arm of force L of centreline space
dAnd imported M in the computing machine 8 in the step [5]
d, and each sample size p that sets and click sum N calculate the adhesion numerical value F that biological sample A and object B should moments by following formula by computing machine 8
a
In the formula, M
pBe the gross mass of light weight liquid, M
p=p * N, g are acceleration of gravity, and its value is 9.8N/kg;
[12] repeated test step [1]-[11] are tested 20 biosome A of the same race altogether, and 20 data of acquisition machine 8 data processing softwares are as calculated handled, and obtain the adhesion mean value, standard variance between this kind biosome A and object B and are printed by printer 10.
Above-mentioned camera 2, temperature sensor 5, humidity sensor 6, electrodynamic type microsyringe 7 (carrying function software), store memory have computing machine 8, display 9, the printer 10 of Flame Image Process and data processing software all to purchase.
Below further specify the present invention by specific embodiment, but embodiment only be used for the explanation, can not limit the scope of the invention.
Embodiment 1:
With the adhesion numerical value measured between the polishing paper that biological sample fruit bat and grain size are 5 μ m is example:
[1] opens computing machine 8 preheatings ten minutes;
[2] but open the dodge gate 102 of the transparent casing 1 of open type, the second lift adjustment screw rod 421 of turn dynamometry induction pieces 4 second jacking gears is with the height of the regulating antenna formula pull bar 41 low 5mm of height to the left end magnetic sheet 411 of the position ratio antenna formula pull bar 41 that makes camera 2; The first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn makes the aspect ratio antenna formula pull bar 41 low 8cm of the loading round platform 31 that has rolling bearing;
[3] according to the desired measuring accuracy 0.2mg of adhesion between biological sample fruit bat and polishing paper, find therewith by table 1 that the antenna formula pull bar 41 required length of precision correspondence are 30cm, its value of the arm of force of then measuring the centreline space of the center line of reservoir 414 of antenna formula lever 41 right-hand members and the second lift adjustment screw rod 421 is L
pIts value of the arm of force of the center line of=11cm and measurement left end magnetic sheet 411 and the centreline space of the second lift adjustment screw rod 421 is L
d=12cm, and with L
p, L
dValue input computing machine 8;
[4] regulate the leveling primary nut 412 at two ends, antenna formula pull bar 41 left and right sides, whether the bubble of observing the level meter 413 at antenna formula pull bar 41 centers regulates the initial balance of antenna formula pull bar 41 in the center;
[5] get one of biological sample fruit bat and claim its mass M
d=1.3mg, should be worth input computing machine 8, with glue fruit bat back and wing are bonded on the existing sample preparation magnetic sheet then, make magnetic sheet 411 adhesives of this sample preparation magnetic sheet and antenna formula pull bar 41 left ends below, fruit bat is suspended on the magnetic sheet 411, thereby antenna formula pull bar 41 left ends is laid particular stress on and slightly downward-sloping;
[6] be that the polishing paper of 5 μ m is placed on rotatable objective table 3 and has on the loading round platform 31 of rolling bearing with grain size, the loading round platform 31 that turn has rolling bearing moves under the magnetic sheet 411 polishing paper, and the first lift adjustment screw rod 321 of first jacking gear of the rotatable objective table 3 of turn makes the loading round platform 31 that has rolling bearing slowly rise to make with fruit bat and just contacts;
[7] but regulate temperature sensor 5, humidity sensor 6 and make that temperature is 25 ℃ in the transparent casing 1 of open type, humidity is 65%; But close the dodge gate 102 of the transparent casing 1 of open type then immediately;
[8] open the function software of the control electrodynamic type microsyringe 7 of computing machine 8, each sample size of electrodynamic type microsyringe 7 is set, and each sample size is the light weight liquid alcohol, each sample size p=1mg, mouse is every to be clicked, with the sample size injection reservoir 414 of this setting;
[9] utilize camera 2 to take the whole loading process of biological sample fruit bats and be presented on the display 9, in this whole shooting process, can preserve arbitrarily one moment picture, machine 8 image processing softwares print through printer 10 after handling as calculated;
[10] action of clicking the mouse in the repeating step [8] when observing the moment that the biological sample fruit bat separates with polishing paper on the loading round platform 31 that has rolling bearing on the display 9, stops to click the mouse;
[11] arm of force L of the centreline space of the center line of the reservoir 414 of antenna formula lever 41 right-hand members by having imported computing machine 8 in the step [3] and the second lift adjustment screw rod 421
pThe arm of force L of the centreline space of the center line of=11cm, left end magnetic sheet 411 and the second lift adjustment screw rod 421
dImported the M in the computing machine 8 in=12cm and the step [5]
d=1.3mg, and each sample size p=1mg that sets and click sum N=32 calculate the adhesion numerical value F that biological sample fruit bat and polishing paper should moments by following formula by computing machine 8
a=0.275mN;
[12] repeated test step [1]-[11], test 20 biological sample fruit bats of the same race altogether, 20 data that obtain machine 8 data processing softwares are as calculated handled, the adhesion mean value that obtains between this kind biological sample fruit bat and this kind polishing paper is that 0.283mN, standard variance are 0.033%, and is printed by printer 10.
Embodiment 2:
Measure the adhesion between the coated abrasive surface that biological sample beetle and grain size are 40 μ m, basic step is identical with embodiment 1, and its difference is:
Selected biological sample is a beetle, and object is that grain size is the sand paper of 40 μ m, according to the measuring accuracy 1.5mg of adhesion between biological sample beetle and sand paper, finds therewith by table 1 that the antenna formula pull bar 41 required length of precision correspondence are 20cm, the L that records
p=8cm, L
d=8cm, M
d=10.7mg injects the sample size p=5mg of reservoir at every turn, and click sum N=57 calculates the adhesion numerical value F that biological sample beetle and sand paper should moments by formula by computing machine 8
a=2.69mN, test 20 biological sample beetles of the same race, 20 data that obtain machine 8 data processing softwares are as calculated handled, and the adhesion mean value that obtains between this kind biological sample beetle and sand paper is 2.71mN, standard variance 0.018%, and is printed by printer 10.
The present invention is a kind of device for measuring force and method of testing of measuring adhesion between biological living and object, solved the difficult problem that forefathers can not the test organisms live body, for the biomechanics measurement provides a kind of effective means, and then be that research biological function mechanism, bionic development and application are laid a good foundation.
Claims (3)
1. device for measuring force of measuring biological living and adhesive force between objects; It comprises camera (2); Temperature sensor (5); Humidity sensor (6); Electrodynamic type microsyringe (7); Store memory has the computer (8) of image processing and data processing software; Display (9); Printer (10); Workbench (11), it is characterized in that it also comprise by be placed on the outer cover (101) on the workbench (11) and be connected through the hinge on outer cover (101) antetheca dodge gate (102) but the transparent casing of open type (1) that forms; By with the first lift adjustment screw rod, (321) the loading round platform with ball bearing that links together on, (31) and by the upper end with the loading round platform of ball bearing, (31) ball bearing link to each other its middle part and the first lift adjustment nut with bracing frame, (322) the first lift adjustment screw rod that links to each other, (321) and middle part and the first lift adjustment screw rod, (321) the first lift adjustment nut with bracing frame that links to each other, (322) its both common rotatable objective tables that form of first lowering or hoisting gear that consists of, (3); Level meter (413) below that the leveling primary nut (412) that is sleeved on Antenna Type pull bar (41) two ends, the left and right sides by the magnetic sheet (411) that is packed in Antenna Type pull bar (41) left end below is packed in Antenna Type pull bar (41) center be packed in Antenna Type pull bar (41) right-hand member and by the reservoir (414) that conduit links to each other with electrodynamic type microsyringe (7) forms and by the middle and lower part be supported on the Antenna Type pull bar (41) of the second lift adjustment screw rod (421) upper end and upper end link to each other with Antenna Type pull bar (41) middle and lower part in the middle part of with the second lift adjustment screw rod (421) that the second lift adjustment nut (422) with bracing frame links to each other in the middle part of its both common dynamometry induction pieces (4) that form of the second lowering or hoisting gear with the second lift adjustment nut (422) formation of bracing frame of linking to each other with the second lift adjustment screw rod (421).
2. a kind of device for measuring force of measuring adhesion between biological living and object according to claim 1, it is characterized in that but the transparent casing of described open type (1) can be made by transparent organic glass or transparent high polymer plastics, its shape can be rectangular parallelepiped or cube, length L is that 40-60cm, width W are that 30-50cm, height H are 30-50cm, places dodge gate (102) length L on outer cover (101) antetheca ' be that 30-50cm, width H ' they are 20-40cm.
3. method of testing of measuring the device for measuring force of adhesion between biological living and object is characterized in that realizing as follows:
[1] opens computing machine (8) preheating ten minutes;
[2] but open the dodge gate (102) of the transparent casing of open type (1), the second lift adjustment screw rod (421) of turn dynamometry induction pieces (4) second jacking gears is with the height of regulating antenna formula pull bar (41) the low 5-10mm of height to the left end magnetic sheet (411) of the position ratio antenna formula pull bar (41) that makes camera (2); The first lift adjustment screw rod (321) of first jacking gear of the rotatable objective table of turn (3) makes the low 8-10cm of the aspect ratio antenna formula pull bar (41) of the loading round platform (31) that has rolling bearing;
[3] according to the desired measuring accuracy of adhesion between biological sample (A) and object, find the required length of antenna formula pull bar (41) of precision correspondence therewith by table 1, its value of the arm of force of then measuring the centreline space of the center line of reservoir (414) of antenna formula lever (41) right-hand member and the second lift adjustment screw rod (421) is L
pWith its value of the arm of force of the center line of measuring left end magnetic sheet (411) and the centreline space of the second lift adjustment screw rod (421) be L
d, and with L
p, L
dValue input computing machine (8);
Table 1
[4] regulate the leveling primary nut (412) at antenna formula pull bar (41) two ends, the left and right sides, whether the bubble of observing the level meter (413) at antenna formula pull bar (41) center regulates the initial balance of antenna formula pull bar (41) in the center;
[5] with biological sample (A), as fly class or cricket or spider or beetle, one that gets certain claims that its quality is M
dAnd with its value input computing machine (8), then this biological sample (A) is fixed on the existing sample preparation magnetic sheet, make magnetic sheet (411) adhesive of this sample preparation magnetic sheet and antenna formula pull bar (41) left end below, biosome sample (A) is suspended on the magnetic sheet (411), thereby antenna formula pull bar (41) left end is laid particular stress on and slightly downward-sloping;
[6] with object (B), as sand paper or polishing paper or glass or plastics or colloid, be placed on the loading round platform (31) that has rolling bearing of rotatable objective table (3), the loading round platform (31) that turn has rolling bearing moves under the magnetic sheet (411) object (B), and the first lift adjustment screw rod (321) of first jacking gear of the rotatable objective table of turn (3) slowly rises to the loading round platform (31) that has rolling bearing to make with biosome (A) and just contacts;
[7] regulate temperature sensor (5), humidity sensor (6) but make the interior ambient temperature and humidity of the transparent casing of open type (1) be controlled at temperature 20-25 ℃, humidity 65-70%; But close the dodge gate (102) of the transparent casing of open type (1) then immediately;
[8] open the function software of the control electrodynamic type microsyringe (7) of computing machine (8), each sample size of electrodynamic type microsyringe (7) is set, each sample size is light weight liquid such as alcohol or low density polyethylene, each sample size p is 0.02mg-2g, mouse is every to be clicked, with the sample size injection reservoir (414) of this setting;
[9] utilize camera (2) to take the whole loading process of biological sample (A) and be presented on the display (9), in this whole shooting process, can preserve arbitrarily one moment picture, print through printer (10) after machine (8) image processing software is handled as calculated;
[10] action of clicking the mouse in the repeating step (8) when observing the moment that biological sample (A) separates with object (B) on the loading round platform (31) that has rolling bearing on the display (9), stops to click the mouse;
[11] arm of force L of the centreline space of the center line of the reservoir (414) of antenna formula lever (41) right-hand member by having imported computing machine (8) in the step [3] and the second lift adjustment screw rod (421)
p, the center line of left end magnetic sheet (411) and the second lift adjustment screw rod (421) the arm of force L of centreline space
dAnd imported M in the computing machine (8) in the step [5]
d, and each sample size p that sets and click sum N, calculate biological sample (A) and object (B) the adhesion numerical value F of this moment by following formula by computing machine (8)
a
In the formula, M
pBe the gross mass of light weight liquid, M
p=p * N, g are acceleration of gravity, and its value is 9.8N/kg;
[12] repeated test step [1]-[11], test 20 biosomes of the same race (A) altogether, 20 data that obtain machine (8) data processing software are as calculated handled, and obtain the adhesion mean value, standard variance between this kind biosome (A) and object (B) and are printed by printer (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810203235 CN101482553B (en) | 2008-11-24 | 2008-11-24 | Measuring apparatus and method for adhesive force between biological living body and object |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810203235 CN101482553B (en) | 2008-11-24 | 2008-11-24 | Measuring apparatus and method for adhesive force between biological living body and object |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101482553A true CN101482553A (en) | 2009-07-15 |
| CN101482553B CN101482553B (en) | 2013-01-16 |
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| CN 200810203235 Expired - Fee Related CN101482553B (en) | 2008-11-24 | 2008-11-24 | Measuring apparatus and method for adhesive force between biological living body and object |
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| CN102252960A (en) * | 2011-04-21 | 2011-11-23 | 西北工业大学 | Device and method for measuring adhesive force between objects |
| CN102262048A (en) * | 2011-04-21 | 2011-11-30 | 西北工业大学 | Device for measuring adhesion between ice and object, and testing method of device |
| CN102589780A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Lever type chain pressurized device and method for measuring climbing force of insect or small animal |
| CN102589776A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Device and method for measuring climbing force of inset in multiple poses and in different climbing ways |
| CN102589779A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Centrifugal device and method for measuring crawling attachment force and gait of insects and small animals |
| CN102578044A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Device and method for measuring creeping probability of insect on rough surface |
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| CN103163069A (en) * | 2013-02-21 | 2013-06-19 | 中南大学 | Method and system for measuring solid material surface adhesion force |
| CN108461889A (en) * | 2018-02-05 | 2018-08-28 | 国网河南省电力公司电力科学研究院 | A kind of fault location antenna leveling slewing equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR100675103B1 (en) * | 2000-07-18 | 2007-01-29 | 주식회사 태산솔루젼스 | Apparatus and method for the quantitative measurement of adhesive force of cell to constructs |
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| CN102252960A (en) * | 2011-04-21 | 2011-11-23 | 西北工业大学 | Device and method for measuring adhesive force between objects |
| CN102262048A (en) * | 2011-04-21 | 2011-11-30 | 西北工业大学 | Device for measuring adhesion between ice and object, and testing method of device |
| CN102252960B (en) * | 2011-04-21 | 2013-02-20 | 西北工业大学 | Device and method for measuring adhesive force between objects |
| CN102589779A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Centrifugal device and method for measuring crawling attachment force and gait of insects and small animals |
| CN102589776A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Device and method for measuring climbing force of inset in multiple poses and in different climbing ways |
| CN102578044A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Device and method for measuring creeping probability of insect on rough surface |
| CN102589777A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Pick-needle type interference device and method for measuring crawling posture and crawling attachment force of insects or small animals |
| CN102589781A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Cantilever beam type measuring device of crawling attaching force of insects or small animals and method thereof |
| CN102589780A (en) * | 2012-02-11 | 2012-07-18 | 东华大学 | Lever type chain pressurized device and method for measuring climbing force of insect or small animal |
| CN102589780B (en) * | 2012-02-11 | 2014-01-08 | 东华大学 | Lever type chain pressurized device and method for measuring climbing force of insect or small animal |
| CN102589781B (en) * | 2012-02-11 | 2014-04-16 | 东华大学 | Cantilever beam type measuring device of crawling attaching force of insects or small animals and method thereof |
| CN102589779B (en) * | 2012-02-11 | 2015-04-08 | 东华大学 | Centrifugal device and method for measuring crawling attachment force and gait of insects and small animals |
| CN103163069A (en) * | 2013-02-21 | 2013-06-19 | 中南大学 | Method and system for measuring solid material surface adhesion force |
| CN103163069B (en) * | 2013-02-21 | 2014-11-05 | 中南大学 | Method and system for measuring solid material surface adhesion force |
| CN108461889A (en) * | 2018-02-05 | 2018-08-28 | 国网河南省电力公司电力科学研究院 | A kind of fault location antenna leveling slewing equipment |
| CN112146997A (en) * | 2020-09-07 | 2020-12-29 | 武汉理工大学 | Magnetic rod similar soil, shear test device and method based on magnetic rod similar soil technology |
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