CN101449738A - Preparation method of probiotics fermentation activity pasty feedstuff general for animal - Google Patents
Preparation method of probiotics fermentation activity pasty feedstuff general for animal Download PDFInfo
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Abstract
The invention discloses an all-purpose preparation method of probiotic fermentation activity pasty feeds for animals, having the steps of: weighing raw materials according to prescription, fermentation base materials mainly comprising raw materials with the following weight percent: corn flour 45-70, bean pulp flour 12-32, rice flour 8-20, wheat flour 0-20, bean powder 0-15; malt 0-8, growth factor 0-0.9; pre-processing fermentation base materials, crushing, extruding and screening; preparing strain of probiotic; inoculating fermentation base materials with seed bacterial liquid, wherein the method comprises the ways of: direct fermentation, namely making paste mash fermentation using seed bacterial liquid, causing moisture content in paste leavening 52-63%; continuous fermentation, converting seed bacterial liquid into paste mash fermentation via liquid state fermentation, using paste leavening as probiotic ferment; adopting gapless packaging bags; standing for fermentation, removing fermentative air, sealing the gapless packaging bags; stopping fermenting; storing by sealing. The inventive feed probiotic is long in storage time, manufacturing quality and effect of the paste leavening are stable.
Description
Technical field:
The present invention relates to a kind of feed producing method, relate in particular to a kind of fermented feed containing probiotics preparation method.
Background technology:
The product of more use is probiotic, Synbiotics etc. in the livestock-raising industry at present, but is subjected to generally to add in the existing feed product antibiotic the influence, and probio is difficult to survive in feed.The probiotics preparation technology of processing of tolerance feed and long-term external preservation does not still pass a test, and vigor is poor, and effect is undesirable.Though the probiotics that has is taked high-tech such as micro-capsule bag quilt, beneficial bacterium especially anaerobic probio survival rate and vigor thereof is also very undesirable, and the holding time is short.By product enter survive in the animal body have only a spot of bacillus, saccharomycete and so on, the biological function effect of actual performance is limited.And for example traditional fermented feed is because facility is simple and crude, and method is original, simple, extensive, and the control of the bioprocess of feed fermentation is not strict, operate lack of standardization, product quality and result of use instability.And at present the industrial fermentation feed only at the utilization of fermenting of feed a part raw material, as fermented bean dregs, the cotton dish dregs of rice, pomace etc.Its defective is to make finished product through heating, drying again after the product fermentation, highly energy-consuming, and also the thermal sensitivity nutrient substance is destroyed.Therefore it is urgent that present probio is applied to the demand that livestock-raising produces, and market is huge.But use probio in order to make additive, not only functional bacterial classification is few, and key is that preparation technique is poor, and many important anaerobic probios are difficult to survival, and many influence factors are difficult to overcome in the use.When it is used as leavening,, have a big risk again because of living contaminants takes place easily in application, probio is used in animal-breeding and still is in the exploration developing stage.Described probio pasty state fermented feed can effectively overcome the various drawbacks that exist in the above beneficial microbe application.Can be economical, convenient, safe, be applied to breeding production efficiently, have practical value.Make the feed acidifying as fermentation, can suppress the pathogenic microorganism growth, create conditions for realizing inactive fodder antibiotics additive.This is to eliminate the prerequisite and basis that livestock products are residual, the control antibody-resistant bacterium produces, realizes environment and food security.Fermented feed directly provides high-activity probiotics and bioactivator thereof, regulates animal health.The feed fermentation technology also can combine with modern food processing technologies such as slaking, enzymolysis, saccharification, thoroughly eliminates the feed ANFs, and the degraded macromolecular substances is excavated the potential nutritional labeling of feed.Make it fully by the animal digestibility and utilization, realize reducing discharging, the pollution that controls environment, save feed resource, reduce aquaculture cost, improve the animal health condition purpose.Fermented feed can be directly works to the balance adjustment of intestinal microecology, be of value to animal immunizing power, anti-stress with grow.Fermented feed helps the optimum recurrence of the little ecology of plant's environment, changes the poor environment situation of present " plant is the plague area " etc.Present in addition feed is that different breed kinds is carried out special configuration, and versatility is bad, can not effectively enhance productivity, and production, transportation and sale be inconvenience also.Also there is not solution well at present.
Summary of the invention:
The anaerobic probio can not survive well in the existing feed in order to overcome, the unsettled technical deficiency of quality of production effect of fermented feed; The object of the invention provides the preparation method of the pasty state fermented feed that a kind of probio can good survival, probiotics fermentation activity pasty feedstuff can be used as the direct feeding animals of formula material, also can be used as the probiotic bacterial classification is used for all feeds raw material is fermented, be the feed of general for animal, and make stay-in-grade pasty state fermented feed suitability for industrialized production, standardized management and commercial application become possibility.
For realizing that the technical scheme that purpose of the present invention is taked is:
A kind of preparation method of probiotics fermentation activity pasty feedstuff of general for animal is characterized in that: described preparation method's step is:
1, takes by weighing fermentation base-material dry in the fermented feed raw material by prescription, the base-material dry that wherein ferments mainly is prepared from by the material component that comprises following weight portion: corn flour 45～70, bean cake powder 12～32, ground rice 8～20, flour 0～20, bean powder 0～15, Fructus Hordei Germinatus 0～8, growth factor 0～0.9;
2, to the pre-treatment of fermentation base-material, the fermentation base-material is pulverized, mixed,, rescreen sheet after expanded, obtain granular material again to fermentation base-material extrusion;
3, single culture or compound multi-strain fermentation are according to circumstances produced in the preparation of probio bacterial classification;
4, with the kind daughter bacteria liquid that obtains in the step 3 to what obtain in the step 2 expanded or micronizing the fermentation base-material inoculate, the mode of employing includes:
4.1.1, direct fermentation, promptly directly make the mass fermentation with inoculation bacterium liquid, make that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
4.1.2, the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
4.2.1, continuous fermentation method, promptly inoculate bacterium liquid and change the mass fermentation through liquid state fermentation, change the mass fermentation after the liquid state fermentation, make that moisture is 65～85% in the liquid state fermentation material, or by 15～45% of fermentation base-material dry matter weight;
4.2.2, the liquid state fermentation material is put standing for fermentation in the fermenting cellar, cultivation, can change mass fermentation;
4.2.3, add and to treat that fermentation materials makes that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
4.2.4, the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
4.3.1, make probiotics leaven with the pasty state fermentation materials, be that masterbatch continuously ferments promptly with the pasty state fermentation materials, the pasty state fermentation materials is inoculated by weight 1:8～30 with granular material to be fermented, make that moisture is 65～85% in the liquid state fermentation material, or by 50～85% of fermentation base-material dry matter weight;
4.3.2, the liquid state fermentation material is put standing for fermentation in the fermenting cellar, cultivation, can change mass fermentation;
4.3.3, by the liquid state fermentation material with treat fermentation materials and 1: 0.45～0.85 be formulated as the pasty state fermentation materials, make that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
4.3.4, the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
5, the pasty state fermentation materials that adopts gapless packaging bags step 4 to obtain;
6, be transported to the fermenting cellar standing for fermentation after pasty state fermentation materials packing is finished, get rid of the gas that fermentation produces in the seamless packaging bag after, seal seamless packaging bag, transport fermenting cellar;
7, the pasty state fermentation materials after the fermentation is stopped fermentation;
8, the pasty state fermented feed behind the seal package is put warehouse storage.
Described preparation method's step is:
1, takes by weighing fermentation base-material dry in the fermented feed raw material by prescription, the base-material dry that wherein ferments is prepared from by the material component that comprises following weight portion: corn flour 45～65, bean cake powder 15～30, ground rice 10～18, flour 5～20, bean powder 0～14, Fructus Hordei Germinatus 0～7, growth factor 0～0.8;
2, to the fermentation base-material pre-treatment,
2.1, the fermentation base-material is selected, removes foreign matter, the mould caking that loses;
2.2, the fermentation base-material is pulverized, expanded raw material uses aperture 2mm sieve sheet, the non-expansion raw material sieves sheet with aperture≤1mm;
2.3, each fermentation base-material is mixed;
2.4, again to fermentation base-material extrusion, expanded condition, 110～145 ℃ of temperature, 3～4.5 atmospheric pressure of steam pressure, extrusion electric machine main shaft rotating speed 400～600 commentaries on classics/per minutes;
2.5, the fermentation base-material after expanded is used the screening of aperture 1.5～2.0mm sieve sheet, obtain granular material;
3, single culture or compound multi-strain fermentation are according to circumstances produced in the preparation of probio bacterial classification, and the probiotics fermentation activity pasty feedstuff bacterial classification is the compound probiotic bacterial classification, and the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 2.2～7.0 * 10
6Cfu/ml, saccharomycete 1.2～3.5 * 10
6Cfu/ml, bacillus subtilis 1.2～3.5 * 10
6Cfu/ml, Bifidobacterium 2.2～7.0 * 10
6Cfu/ml makes kind of a daughter bacteria liquid;
4, with the kind daughter bacteria liquid that obtains in the step 3 to what obtain in the step 2 expanded or micronizing the fermentation base-material inoculate, the mode of employing is one of following mode:
4.1.1, direct fermentation, promptly directly make the mass fermentation with inoculation bacterium liquid, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
4.1.2, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
4.2.1, continuous fermentation method, promptly inoculate bacterium liquid and change the mass fermentation through liquid state fermentation, change the mass fermentation after the liquid state fermentation, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 70～85% in the liquid state fermentation material, or by 20～43% of fermentation base-material dry matter weight, stir, the pasty state fermentation materials should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
4.2.2, the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20～60 hours standing for fermentation, cultivation, can change the mass fermentation;
4.2.3, add and to treat that fermentation materials makes that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
4.2.4, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
4.3.1, make probiotics leaven with the pasty state fermentation materials, be that masterbatch continuously ferments promptly with the pasty state fermentation materials, the pasty state fermentation materials is inoculated by weight 1: 10～25 with granular material to be fermented, with conventional running water preparation liquid state fermentation material, make that moisture is 70～85% in the liquid state fermentation material, or by 20～43% of fermentation base-material dry matter weight, stir, the liquid state fermentation material should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
4.3.2, the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20～60 hours standing for fermentation, cultivation, can change the mass fermentation;
4.3.3, by the liquid state fermentation material with treat that fermentation materials 1:0.45～0.70 is formulated as the pasty state fermentation materials, make that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
4.3.4, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
5, adopt by the packaging bag of three layers of bag membrane orienting exhaust, the pasty state fermentation materials that packaging step 4 obtains;
6, be transported to fermenting cellar after pasty state fermentation materials packing is finished, be staggered pile, 30～42 ℃ of environment temperatures, 30～50 hours standing for fermentation is got rid of the gas that fermentation produces in the mesopelagic layer bag, with rubber band lock ring mesopelagic layer sack, transports fermenting cellar system ferment;
7, to the feedstock property of the pasty state fermentation materials after the fermentation according to the fermentation base-material, adopt the method for nature system ferment and vacuum-packed system ferment to stop fermentation respectively,
7.1, carry out nature system ferment when the fermented feed raw material is thick matter cheap raw material, even it fully ferments, treat that fermentation materials is low to acidity because of fermenting, i.e. PH≤4.0,2～4 day, seal package again when microorganism stops growing;
7.2, when the fermented feed raw material is concentrated feed, carried out appropriate fermentation, promptly fermented 1～2 day, make microbial growth stop at the later stage of logarithmic growth, guarantee the quality with vacuum packaging system ferment, vacuum-packed at once after promptly transporting from fermenting cellar;
8, the pasty state fermented feed behind the seal package is put warehouse storage, special warehouse, normal temperature storage, routine is piled up, environment clean and dry, shady and cool ventilation.
The fermentation base-material basic nutrition content of described fermented feed raw material: crude protein 〉=16.0%, lysine 〉=0.7%, egg+cystine 〉=0.5%, threonine 〉=0.5%; Calcium 〉=0.09%; Total phosphorus 〉=0.3%.
The fermentation base-material dry of described fermented feed raw material is prepared into by the material component of following weight portion: the fermentation base-material dry of fermented feed raw material is prepared into by the material component of following weight portion: corn flour 53, bean cake powder 25, ground rice 15, flour 12, bean powder 9, Fructus Hordei Germinatus 6, growth factor 0.5; Moisture 57% in the pasty state fermentation materials, or by fermentation base-material dry matter weight 46%.
The inoculative proportion of described probio bacterial classification is a lactobacillus plantarum 5.5 * 10
6Cfu/ml, saccharomycete 2.5 * 10
6Cfu/ml, bacillus subtilis 2.2 * 10
6Cfu/ml, Bifidobacterium 3.5 * 10
6Cfu/ml.
Described when making probiotics leaven with the pasty state fermentation materials, the fermentation base-material must be expanded.
Described pasty state fermentation materials must not continuously ferment above twice as leavening.
Describedly produce the amplification bacterial classification as leavening, after bacterial classification is amplified in liquid state fermentation, change the mass fermentation with the pasty state fermentation materials;
Directly make the liquid state fermentation feed with the pasty state fermentation materials as leavening;
Produce wet-milling shape fermented feed, moisture content 20～42% with amplifying again behind the continuous fermentation method production liquid state fermentation material;
Amplify production wet-milling shape fermented feed, moisture content 20～42% with the pasty state fermented feed.
The packaging bag of described three layers of bag membrane orienting exhaust comprises three layers of inner bags, mesopelagic layer bag, outer membrane bag; Inner bag is an air permeable waterproof formula PE plastic foil, and air permeable waterproof formula PE plastic foil is close to pasty state fermented feed surface, and is seamless packing between the pasty state fermented feed; The mesopelagic layer bag is the food fresh keeping PE plastic foil of waterproof gas formula, and fermentation gas is too fast, or gas production is when excessive, can directly emit the fermentation gas that accumulates in the mesopelagic layer bag and in time seals/tying; The outer membrane bag is an outer packaging bag.
The product design of described pasty state fermented feed is rolled into square, cuboid, cylinder or sphere at least.
Basic principle:
Feed of the present invention is the biotransformation that utilizes probiotic cell and enzyme, with the conventional raw material feed of extrusion mode pre-treatment at specific pasty state condition bottom fermentation, make the decomposition of fermented feed raw material mix composition, degrade or change into new nutritional labeling, become and be easy to digestibility and utilization, benefit animal health, have the novel fodder raw material of the general for animal of noresidue, low emission, the little Ecology Action of adjusting.And the probio special life condition that constitutes at the pasty state fermented feed of the activity of anaerobic probio (the special ecological environment that the pasty state fermentation materials provides: have density greatly and extremely abundant complicated feed nutrition material especially, about moisture 55%, water content〉1.2g/g, the dry meter, its moisture state is constraint water and anelasticity Free water, water activity〉9.0) can be bred in a large number and long preservation down, become the probiotics leaven of anaerobic fermented feed containing probiotics.
Physicochemical character:
Probiotics fermention high activity mass water content about 55% leaves standstill not have and separates out water or free water.Be distinctive cadmium yellow color and luster of fermented feed raw material or light lark, have sweet-smelling and sour-sweet fragrance.Protein in the fermented feed raw material, decomposition, degraded and the conversion in various degree of starch polymer nutrient material quilt.Trypsin ihhibitor, SNSP ANFs obviously reduce, active paste type feed PH≤4.5.The organic acid that fermentation produces is mainly lactic acid, acetate, and wherein total lactic acid 〉=1.0%, the digestibility of main nutrient matter such as thick protein, carbohydrate in the animal daily ration is more than 88%.The active shallow top layer of pasty state fermentation materials flora is mainly saccharomycete, bacillus aerobic probio and a small amount of anaerobic probio, and the deep layer flora is mainly the anaerobic probio and a small amount of aerobic probio of lactic acid bacteria, Bifidobacterium, streptococcus fecalis.The nutritional quality characteristic of pasty state fermentation materials keeps keeping probiotic active (more than 1 year) for a long time through the probio pasty state fermented feed after the vacuum packaging more than 60 days under the conventional storage conditions.
Fundamental characteristics:
Process with conventional feed, physical action by powder essence, mixing machinery effect or baking, expanded, modulation, the heating power of granulating is compared, this pasty state fermented feed is by the biofermentation effect to the fermented feed raw material, makes the fermented feed material composition decompose, degrade, change into the variation of new nutrient biochemistry.
Application process:
Feed of the present invention can be used as the direct feeding animals of formula material, and the effect of the functional feed of animal microecological is regulated in performance; Also can be used as the probiotic bacterial classification and be used for all feeds raw material is fermented, improve the nutritional quality of feedstuff, the effect of performance feed fermentation agent.
1, the pasty state fermented feed uses as formula material:
The active paste type fermented feed is the fermented feed of various general for animal, when being directly used in preparation animal adequate diet, at first according to animal species, growth phase and production needs, determines suitable cooperation ratio.Basic feedstuff in the alternative generally speaking complete feed (as corn, dregs of beans) 50% (be on dry matter basis, 40～60%, down with), adding proportion accounts for 30% (15～45%) of full price ration formulation.Proportioning in special daily ration can strengthen plant-derived energy feed of cooperation ratio or replacing whole and protein feeds such as corn, dregs of beans as the wean anti-stress feed.In addition, vitamin, mineral matter and trace element must add supporting premix or concentrate feed, make it to meet the nutritional need level.
2, use as the feed fermentation agent:
Be that masterbatch carries out liquid state fermentation promptly with the pasty state fermented feed, pasty state fermented feed and granular material 1:10～20 inoculations by weight to be fermented, adding running water mixes, the moisture of all materials is 80% (70～85%) during liquid state fermentation, stir, all liquid state fermentation materials are wanted containing in the inoculation bacterium liquid of leavening through bubble fully, and there is height 〉=5.0cm inoculation bacterium liquid on its top layer as air barrier.Fermentation is under 25～40 ℃ of indoor environment temperatures, cultivates through 24～48 hours standing for fermentation to get final product, and this liquid state fermentation material can directly add other feedstuffs and be made into complete diet pellet and feed.When being used to prepare the animal adequate diet, should be according to animal species, growth phase and production needs, determine suitable cooperation ratio, configuration proportion accounts for 25% of basic feedstuff in the animal complete feed (as corn, dregs of beans) and (is on dry matter basis generally speaking, 10～40%), account for 20% (10～30%) of full price ration formulation, proportioning in special daily ration such as sow mixed feed, can strengthen plant-derived energy feed of cooperation ratio or replacing whole and protein feeds, as corn, dregs of beans.In addition, vitamin, mineral matter and trace element must add supporting premix or concentrate feed makes it to meet the nutritional need level.
Quality restriction:
1, aesthetic quality: after the pasty state fermentation materials opening encapsulation packing, do not have and ooze out free water, appearance is moistening, and the quality softness is faint yellow or yellow-white, the extremely thin mucus in shallow top layer (viable bacteria), no mildew or other color spots distribute sweet-smelling or sour-sweet fragrance, do not have corrupt peculiar smell, the visible deep layer material softness in place that fractures is moistening, is the vivid color and luster of raw material that sets out that ferments.
2, microscopy: the mirror hypothallus is consistent with the fermentation bacterial classification form that connects, as visible a large amount of saccharomycete on the shallow top layer of pasty state fermentation materials, bacillus, Aspergillus aerobic probio; The thalline of deep layer visible intensive and lactic acid bacteria Anaerobic Bacteria homomorphosis inoculation.
3, special index: total amount of probiotics 〉=1 * 10
8/ g, acidity index PH≤4.0, total lactic acid 〉=1.0%.
4, nutritive index:
Dry 45%: thick protein 9.5%, crude fibre 2.5%, crude fat 1.8%, ash content 2.6%, insoluble ash content 1.2%, energy digestibility 89.5%, protein digestibility 92.7%, fat digestibility 86.0%, total phosphorus digestibility 80.5%.
Application example: (probio pasty state active fermentation feed is raised weanling pig)
For a long time, Swine Production is to overcome the influence of early weaning irritability syndrome to the pigling production performance, people to piglet physiology, raw material select, the diet design done big quantity research with the feeding environment aspect, and health and production performance behind the early weaning piglet are obviously improved.But totally see, because the condition of raising in the reality is poor, backward in management, more important is high-quality animal-based protein matter feed deficiency, prescription and production and processing are backward in technique, cause in the actual production behind the weaned piglet nutrition not have guarantee, trophism stress be also in various degree ubiquity.Because weaned piglet stage premunition descends, and people are taken place for controlling disease, and general excess is added antibiotic medicine in the feed, making piglet produce becomes the severely afflicated area that fodder antibiotics is abused again.
At the problems referred to above, carried out the weaning pig feeding test with described probio pasty state active fermentation feed.90 of 28 age in days weanling pigs are selected in test, are divided into test group one, test group two and control group totally three groups at random by parity, sex, body weight phase approximately principle.Every group of 3 groups, 10 pigs of each group.Test group daily ration prescription and the equal trophic level of control group, test group one feed formula is a corn 42%, dregs of beans 18%, expanded soybean 13%, popcorn 11%, whey powder 6%, fish meal 6%, piglet concentrate feed 4% (containing vitamin, mineral matter, amino acid, flavor enhancement, enzyme preparation, medicated premix, carrier).Corn, dregs of beans consumption in test group dual-purpose 30% (being on dry matter basis) probiotic active pasty state fermented feed alternate test group one prescription, promptly corn 21%, dregs of beans 9%, fermentation material 30%, expanded soybean 13%, popcorn 11%, whey powder 6%, fish meal 6%, piglet premix 4%.Enzyme-containing formulation not in the 4% supporting weanling pig premix does not contain any antibiotic.Control group marketing pellet.
Through 28 days feeding experiments:
Compare for one group with test, the speed of growth improves 6.8% (daily gain 287 grams), and feed conversion rate improves 12.3% (feed increases weight than 1.25:1), and daily ingestion amount improves 4.6% (daily ingestion amount 359 grams); Compare with control group, the speed of growth improves 3.3%, and feed conversion rate improves 15.3%, and daily ingestion amount improves 5.6%.
The result shows the pasty state fermented feed through probiotics fermention, improve, improved the nutritional quality of vegetality feedstuff, make it to be easier to by the weanling pig digestibility and utilization, can reduce animal derived feed consumption (as SDPP, skimmed milk power) in the weanling pig feed formula, reduce production costs, to strengthening piglet disease-resistant power, improve health conditions, further bring into play the productive potentialities of piglet and improve environmental ecology all having produced remarkable effect.
The invention has the beneficial effects as follows:
As novel fermentation feed, compared many-sided advantage: 1, the biological content height of active anaerobic probio with the powdery microbe additive with microecologic regulator function; 2, the active anaerobic probio holding time is long; 3, as anaerobic probio special ecological (depositing) environment, the pasty state fermentation materials is different from general carrier, and the conventional feed raw material that is fermented has the functional feed effect; 4, production cost is low; 5, technology, equipment are uncomplicated; 6, easy to use; 7, can improve other feedstuff qualities in the prescription, be sour power as what improve mixed feed; 8, the sweat control operation is simple; 9, the product activity quality is easy to the observation judge; 10, promptly begin to bring into play prebiotic effect (effect is fast) after animal searches for food.
The special pasty state fermentation materials type volume of described probio pasty state active fermentation feed is little; the quality softness; be easy to packing and accumulating; can carry out large-scale production in fermentation base or feed factory; and as terminal use's plant; can directly prepare the complete diet pellet feeding animals with described pasty state fermented feed, thereby simplify raiser's fermentation operation greatly, pasty state fermented feed suitability for industrialized production, standardized management and industrialization development are used becomes possibility.
The specific embodiment:
The present invention is further detailed explanation below in conjunction with embodiment.
Make by the inventive method and prescription (or similar methods and prescription, probio bacterial classification) and to do or wet powdery, granulated meal also is protection scope of the present invention.
Embodiment 1:(unit kilogram)
Fermentation base-material dry in the fermented feed raw material of the embodiment of the invention is prepared from by the material component that comprises following weight portion: corn flour 45, bean cake powder 12, ground rice 8.
Moisture 45% in the pasty state fermentation materials, or by fermentation base-material dry matter weight 40%.
The preparation method's of the embodiment of the invention step is: (unit kilogram)
1, take by weighing fermentation base-material dry in the fermented feed raw material by prescription:
The fermentation base-material basic nutrition content of fermented feed raw material: crude protein 〉=16.0%, lysine 〉=0.7%, egg+cystine 〉=0.5%, threonine 〉=0.5%; Calcium 〉=0.09%; Total phosphorus 〉=0.3%.
1.1, the material quality quality meets corresponding national feedstuff quality standard, meets GB/T17890-1999 as corn, more than 2 grades, more than the dregs of beans NY/T2 level;
1.2, fermentation materials raw material proportioning does suitably to adjust by its moisture, water absorbing force size and product needed;
1.3, the fermentation materials raw material is formed in the prescription kind can do suitably to adjust by production purpose, product needed and local feed resource characteristics;
1.4, can allocate the mineral matter and the trace element of animal requirement into and ferment according to producing needs.
2, to the fermentation base-material pre-treatment,
2.1, the fermentation base-material is selected, removes foreign matter, the mould caking that loses;
2.2, the fermentation base-material is pulverized, expanded raw material uses aperture 2mm sieve sheet, the non-expansion raw material sieves sheet with aperture≤1mm;
2.3, each fermentation base-material is mixed;
2.4, again to fermentation base-material extrusion, expanded condition, 110～145 ℃ of temperature, 3 atmospheric pressure of steam pressure, extrusion electric machine main shaft rotating speed 400～600 commentaries on classics/per minutes;
Described when making probiotics leaven with the pasty state fermentation materials, the fermentation base-material must be expanded;
Described pasty state fermentation materials must not continuously ferment above twice as leavening;
2.5, expanded fermentation base-material is used the screening of aperture 1.5～2.0mm sieve sheet, obtain granular material.
3, single culture or compound multi-strain fermentation are according to circumstances produced in the preparation of probio bacterial classification.
The preparation of probio bacterial classification comprises following link: the seed culture of seed inclined-plane, dull and stereotyped cultivation, Liquid Culture, expansion, the preparation of seeding bacterium liquid.The probiotics fermentation activity pasty feedstuff bacterial classification is the compound probiotic bacterial classification, and the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 2.2 * 10
6Cfu/ml, saccharomycete 1.2 * 10
6Cfu/ml, bacillus subtilis 1.2 * 10
6Cfu/ml, Bifidobacterium 2.2 * 10
6Cfu/ml makes kind of a daughter bacteria liquid.
4, with the kind daughter bacteria liquid that obtains in the step 3 the expanded back or the micronized fermentation base-material that obtain in the step 2 are inoculated, the mode of employing is one of following mode:
4.1.1, direct fermentation, promptly directly make the mass fermentation with inoculation bacterium liquid, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 45% in the pasty state fermentation materials, or by 40% of fermentation base-material dry matter weight;
4.1.2, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible.
4.2.1, continuous fermentation method, promptly inoculate bacterium liquid and change the mass fermentation through liquid state fermentation, change the mass fermentation after the liquid state fermentation, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 65% in the liquid state fermentation material, or by 15% of fermentation base-material dry matter weight, stir, the liquid state fermentation material should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
4.2.2, the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20 hours standing for fermentation, cultivation, can change the mass fermentation;
4.2.3, add and to treat that fermentation materials makes that moisture is 45% in the pasty state fermentation materials, or by 40 of fermentation base-material dry matter weight;
4.2.4, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible.
4.3.1, make probiotics leaven with the pasty state fermentation materials, be that masterbatch continuously ferments promptly with the pasty state fermentation materials, the pasty state fermentation materials is inoculated by weight 1:8 with granular material to be fermented, with conventional running water preparation liquid state fermentation material, make that moisture is 65% in the liquid state fermentation material, or by 50% of fermentation base-material dry matter weight, stir, the liquid state fermentation material should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
4.3.2, the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20 hours standing for fermentation, cultivation, can change the mass fermentation;
4.3.3, by the liquid state fermentation material with treat that fermentation materials 1:0.45 is formulated as the pasty state fermentation materials, make that moisture is 45% in the pasty state fermentation materials, or by 40% of fermentation base-material dry matter weight;
4.3.4, the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible.
Describedly produce the amplification bacterial classification as leavening, after bacterial classification is amplified in liquid state fermentation, change the mass fermentation with the pasty state fermentation materials.
Directly make the liquid state fermentation feed with the pasty state fermentation materials as leavening.
Produce wet-milling shape fermented feed, water content 20% with amplifying again behind the continuous fermentation method production liquid state fermentation material.
Amplify production wet-milling shape fermented feed, moisture content 20% with the pasty state fermented feed.
5, adopt by the packaging bag of three layers of bag membrane orienting exhaust, the pasty state fermentation materials that packaging step 4 obtains.
The packaging bag of described three layers of bag membrane orienting exhaust comprises three layers of inner bags, mesopelagic layer bag, outer membrane bag.Inner bag is an air permeable waterproof formula PE plastic foil, and air permeable waterproof formula PE plastic foil is close to pasty state fermented feed surface, and is seamless packing between the pasty state fermented feed.And interior film quality meets feed and food hygiene requirement, and the gas that fermentation produces can be rejected to outside the film.The mesopelagic layer bag is the food fresh keeping PE plastic foil of waterproof gas formula, seals/only allows behind the tying air and flow out (fermentation gas in the bag is due to gas pressure intensity increases) from inside to outside, can intercept external environment air and oxygen and contact with the pasty state fermentation materials.Fermentation gas is too fast, or gas production is excessive, directly emits the fermentation gas that accumulates in the mesopelagic layer bag and also in time seals/tying.The outer membrane bag is an outer packaging bag.Outer membrane bag major function is a product protection, can stand carrying in accumulating and the application process, collision, carries out vacuum-packed or conventional packing according to product performance and production needs.That container dimensions will be beneficial to keep a public place clean is antifouling, make things convenient for accumulating to be principle with using.
The product design of described pasty state fermented feed is rolled into square, cuboid, cylinder or sphere at least.
6, be transported to fermenting cellar after pasty state fermentation materials packing is finished, be staggered pile, 30～42 ℃ of environment temperatures, 30 hours standing for fermentation is got rid of the gas that fermentation produces in the mesopelagic layer bag, with rubber band lock ring mesopelagic layer sack, transports fermenting cellar system ferment.
7, to the feedstock property of the pasty state fermentation materials after the fermentation according to the fermentation base-material, adopt the method for nature system ferment and vacuum-packed system ferment to stop fermentation respectively,
7.1, carry out nature system ferment when the fermented feed raw material is thick matter cheap raw material, even it fully ferments, treat that fermentation materials is low to acidity because of fermenting, PH≤4.0,2 day, seal package again when microorganism stops growing;
7.2, when the fermented feed raw material is concentrated feed, carried out appropriate fermentation, promptly fermented 1 day, make microbial growth stop at the later stage of logarithmic growth, guarantee the quality with vacuum packaging system ferment, vacuum-packed at once after promptly transporting from fermenting cellar.
8, the pasty state fermented feed behind the seal package is put warehouse storage, special warehouse, normal temperature storage, routine is piled up, environment clean and dry, shady and cool ventilation.
Embodiment 2:(unit kilogram)
Fermentation base-material dry in the fermented feed raw material of the embodiment of the invention is prepared from by the material component that comprises following weight portion: corn flour 70, bean cake powder 32, ground rice 20, flour 20, bean powder 15, Fructus Hordei Germinatus 8, growth factor 0.9.
Moisture 65% in the pasty state fermentation materials, or by fermentation base-material dry matter weight 70%.
The preparation method's of the embodiment of the invention step is: (unit kilogram)
1, take by weighing fermentation base-material dry in the fermented feed raw material by prescription:
2, to the fermentation base-material pre-treatment,
The main contents of this step are with embodiment 1, but expanded condition, 120～140 ℃ of temperature, 4.5 atmospheric pressure of steam pressure, extrusion electric machine main shaft rotating speed 450～600 commentaries on classics/per minutes.
3, the preparation of probio bacterial classification:
The main contents of this step are with embodiment 1, but the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 7.0 * 10
6Cfu/ml, saccharomycete 3.5 * 10
6Cfu/ml, bacillus subtilis 3.5 * 10
6Cfu/ml, Bifidobacterium 7.0 * 10
6Cfu/ml.
4, inoculate with the kind daughter bacteria liquid that obtains in the step 3 fermentation base-material expanded to what obtain in the step 2, the mode of employing is one of following mode:
4.1, direct fermentation,
The main contents of this step are with embodiment 1, and moisture is 65% in the pasty state fermentation materials but make, or by 70% of fermentation base-material dry matter weight.
4.2, continuous fermentation method,
The main contents of this step are with embodiment 1, and moisture is 85% in the liquid state fermentation material but make, or by 45% of fermentation base-material dry matter weight;
The liquid state fermentation material is put in the fermenting cellar under 25～42 ℃ of temperature, cultivated through 60 hours standing for fermentation;
Add and to treat that fermentation materials makes that moisture is 65% in the pasty state fermentation materials, or by 70% of fermentation base-material dry matter weight;
4.3, make probiotics leaven with the pasty state fermentation materials,
The main contents of this step are with embodiment 1, but pasty state fermentation materials and granular material to be fermented be by weight the 1:30 inoculation, make that moisture is 85% in the liquid state fermentation material, or by 85% of fermentation base-material dry matter weight;
The liquid state fermentation material is put in the fermenting cellar under 25～42 ℃ of temperature, cultivated through 60 hours standing for fermentation;
By the liquid state fermentation material with treat that fermentation materials 1:0.85 is formulated as the pasty state fermentation materials, make that moisture is 65% in the pasty state fermentation materials, or by 70% of fermentation base-material dry matter weight.
Produce wet-milling shape fermented feed, moisture content 42% with amplifying again behind the continuous fermentation method production liquid state fermentation material.
Amplify production wet-milling shape fermented feed, moisture content 42% with the pasty state fermented feed.
5, adopt by the packaging bag of three layers of bag membrane orienting exhaust, the pasty state fermentation materials that packaging step 4 obtains.
The substance of this step is with embodiment 1.
6, be transported to the fermenting cellar fermentation after pasty state fermentation materials packing is finished,
The main contents of this step are with embodiment 1, but 34～39 ℃ of environment temperatures, 50 hours standing for fermentation.
7, to the feedstock property of the pasty state fermentation materials after the fermentation according to the fermentation base-material, adopt the method for nature system ferment and vacuum-packed system ferment to stop fermentation respectively,
7.1, naturally the system ferment,
The main contents of this step are with embodiment 1, but treat that fermentation materials is low to acidity because of fermenting, i.e. PH≤4.0,4 day, seal package again when microorganism stops growing;
7.2, appropriateness fermentation,
The main contents of this step are with embodiment 1, but ferment 2 days.
8, seal storage,
The substance of this step is with embodiment 1.
Embodiment 3:(unit kilogram)
Fermentation base-material dry in the fermented feed raw material of the embodiment of the invention is prepared into by the material component that comprises following weight portion: corn flour 53, bean cake powder 25, ground rice 15, flour 12, bean powder 9, Fructus Hordei Germinatus 6, growth factor 0.5.
Moisture 57% in the pasty state fermentation materials, or by fermentation base-material dry matter weight 46%.
The preparation method's of the embodiment of the invention step is: (unit kilogram)
1, prescription takes by weighing the fermentation base-material dry in the fermented feed raw material:
2, to the fermentation base-material pre-treatment,
The main contents of this step are with embodiment 1, but expanded condition, 120～140 ℃ of temperature, 4 atmospheric pressure of steam pressure, extrusion electric machine main shaft rotating speed 500～600 commentaries on classics/per minutes.
3, the preparation of probio bacterial classification:
The main contents of this step are with embodiment 1, but the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 5.5 * 10
6Cfu/ml, saccharomycete 2.5 * 106cfu/ml, bacillus subtilis 2.2 * 10
6Cfu/ml, Bifidobacterium 3.5 * 10
6Cfu/ml.
4, inoculate with the kind daughter bacteria liquid that obtains in the step 3 fermentation base-material expanded to what obtain in the step 2, the mode of employing is one of following mode:
4.1, direct fermentation,
The main contents of this step are with embodiment 1, and moisture is 57% in the pasty state fermentation materials but make, or by 46% of fermentation base-material dry matter weight.
4.2, continuous fermentation method,
The main contents of this step are with embodiment 1, and moisture is 80% in the liquid state fermentation material but make, or by 40% of fermentation base-material dry matter weight;
The liquid state fermentation material is put in the fermenting cellar under 25～42 ℃ of temperature, cultivated through 40 hours standing for fermentation;
Add and to treat that fermentation materials makes that moisture is 59% in the pasty state fermentation materials, or by 50% of fermentation base-material dry matter weight;
4.3, make probiotics leaven with the pasty state fermentation materials,
The main contents of this step are with embodiment 1, but pasty state fermentation materials and granular material to be fermented be by weight inoculation in 1: 22, make that moisture is 80% in the liquid state fermentation material, or by 40% of fermentation base-material dry matter weight;
The liquid state fermentation material is put in the fermenting cellar under 25～42 ℃ of temperature, cultivated through 40 hours standing for fermentation;
By the liquid state fermentation material with treat that fermentation materials 1:0.60 is formulated as the pasty state fermentation materials, make that moisture is 58% in the pasty state fermentation materials, or by 50% of fermentation base-material dry matter weight.
Produce wet-milling shape fermented feed, moisture content 37% with amplifying again behind the continuous fermentation method production liquid state fermentation material.
Amplify production wet-milling shape fermented feed, moisture content 37% with the pasty state fermented feed.
5, adopt by the packaging bag of three layers of bag membrane orienting exhaust, the pasty state fermentation materials that packaging step 4 obtains.
The substance of this step is with embodiment 1.
6, be transported to the fermenting cellar fermentation after pasty state fermentation materials packing is finished,
The main contents of this step are with embodiment 1, but 34～39 ℃ of environment temperatures, 42 hours standing for fermentation.
7, to the feedstock property of the pasty state fermentation materials after the fermentation according to the fermentation base-material, adopt the method for nature system ferment and vacuum-packed system ferment to stop fermentation respectively,
7.1, naturally the system ferment,
The main contents of this step are with embodiment 1, but treat that fermentation materials is low to acidity because of fermenting, i.e. PH≤4.0,3.2 day, seal package again when microorganism stops growing;
7.2, appropriateness fermentation,
The main contents of this step are with embodiment 1, but ferment 1.5 days.
8, seal storage,
The substance of this step is with embodiment 1.
Described probiotics fermentation activity pasty feedstuff water content 57% leaves standstill not have and separates out water or free water.Be distinctive cadmium yellow color and luster of fermented feed raw material or light lark, has sweet-smelling and sour-sweet fragrance, the shallow top layer of pasty state fermentation materials flora is mainly saccharomycete, bacillus aerobic probio and a small amount of anaerobic probio, deep layer flora are mainly lactic acid bacteria, Bifidobacterium anaerobic probio and a small amount of aerobic probio.Be used to prepare the antibiotic-free feed of various animals and as the feed fermentation agent.
Claims (10)
1, a kind of preparation method of probiotics fermentation activity pasty feedstuff of general for animal is characterized in that: described preparation method's step is:
[1], takes by weighing fermentation base-material dry in the fermented feed raw material by prescription, the base-material dry that wherein ferments mainly is prepared from by the material component that comprises following weight portion: corn flour 45～70, bean cake powder 12～32, ground rice 8～20, flour 0～20, bean powder 0～15, Fructus Hordei Germinatus 0～8, growth factor 0～0.9;
[2], to the pre-treatment of fermentation base-material, the fermentation base-material is pulverized, mix, again to fermentation base-material extrusion, rescreen sheet after expanded, obtain granular material;
[3], the preparation of probio bacterial classification, according to circumstances produce single culture or compound multi-strain fermentation;
[4], with the kind daughter bacteria liquid that obtains in the step [3] to what obtain in the step [2] expanded or micronizing the fermentation base-material inoculate, the mode of employing includes:
[4.1.1], direct fermentation are promptly directly made the mass fermentation with inoculation bacterium liquid, make that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
[4.1.2], the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
[4.2.1], continuous fermentation method are promptly inoculated bacterium liquid and are changeed the mass fermentation through liquid state fermentation, change the mass fermentation after the liquid state fermentation, make that moisture is 65～85% in the liquid state fermentation material, or by 15～45% of fermentation base-material dry matter weight;
[4.2.2], the liquid state fermentation material is put standing for fermentation in the fermenting cellar, cultivation, can change mass fermentation;
[4.2.3], add and to treat that fermentation materials makes that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
[4.2.4], the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
[4.3.1], make probiotics leaven with the pasty state fermentation materials, be that masterbatch continuously ferments promptly with the pasty state fermentation materials, the pasty state fermentation materials is inoculated by weight 1:8～30 with granular material to be fermented, make that moisture is 65～85% in the liquid state fermentation material, or by 50～85% of fermentation base-material dry matter weight;
[4.3.2], the liquid state fermentation material is put standing for fermentation in the fermenting cellar, cultivation, can change mass fermentation;
[4.3.3], by the liquid state fermentation material with treat fermentation materials and 1: 0.45～0.85 be formulated as the pasty state fermentation materials, make that moisture is 45～65% in the pasty state fermentation materials, or by 40～70% of fermentation base-material dry matter weight;
[4.3.4], the pasty state fermentation materials stirred after, the compacting material is got rid of the intrapore gas of material as far as possible;
[5], the pasty state fermentation materials that adopts gapless packaging bags step [4] to obtain;
[6], pasty state fermentation materials packing is transported to the fermenting cellar standing for fermentation after finishing, get rid of the gas that fermentation produces in the seamless packaging bag after, seal seamless packaging bag, transport fermenting cellar;
[7], the pasty state fermentation materials after the fermentation is stopped fermentation;
[8], the pasty state fermented feed behind the seal package is put warehouse storage.
2, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 is characterized by:
Described preparation method's step is:
[1], takes by weighing fermentation base-material dry in the fermented feed raw material by prescription, the base-material dry that wherein ferments is prepared from by the material component that comprises following weight portion: corn flour 45～65, bean cake powder 15～30, ground rice 10～18, flour 5～20, bean powder 0～14, Fructus Hordei Germinatus 0～7, growth factor 0～0.8;
[2], to the fermentation base-material pre-treatment,
[2.1], the fermentation base-material is selected, removes foreign matter, the mould caking that loses;
[2.2], the fermentation base-material is pulverized, expanded raw material uses aperture 2mm sieve sheet, and the non-expansion raw material sieves sheet with aperture≤1mm;
[2.3], each fermentation base-material is mixed;
[2.4], again to fermentation base-material extrusion, expanded condition, 110～145 ℃ of temperature, 3～4.5 atmospheric pressure of steam pressure, extrusion electric machine main shaft rotating speed 400～600 commentaries on classics/per minutes;
[2.5], using aperture 1.5～2.0mm to sieve sheet to the fermentation base-material after expanded screens, obtains granular material;
[3], the preparation of probio bacterial classification, according to circumstances produce single culture or compound multi-strain fermentation, the probiotics fermentation activity pasty feedstuff bacterial classification is the compound probiotic bacterial classification, the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 2.2～7.0 * 10
6Cfu/ml, saccharomycete 1.2～3.5 * 10
6Cfu/ml, bacillus subtilis 1.2～3.5 * 10
6Cfu/ml, Bifidobacterium 2.2～7.0 * 10
6Cfu/ml makes kind of a daughter bacteria liquid;
[4], with the kind daughter bacteria liquid that obtains in the step [3] to what obtain in the step [2] expanded or micronizing the fermentation base-material inoculate, the mode of employing is one of following mode:
[4.1.1], direct fermentation, promptly directly make the mass fermentation with inoculation bacterium liquid, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
[4.1.2], the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
[4.2.1], continuous fermentation method, promptly inoculate bacterium liquid and change the mass fermentation through liquid state fermentation, change the mass fermentation after the liquid state fermentation, calculate inoculation bacterium amount by fermentation base-material weight, preparation inoculation bacterium liquid, making pasty state fermentation materials water is conventional running water, make that moisture is 70～85% in the liquid state fermentation material, or by 20～43% of fermentation base-material dry matter weight, stir, the pasty state fermentation materials should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
[4.2.2], the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20～60 hours standing for fermentation, cultivation, can change the mass fermentation;
[4.2.3], add and to treat that fermentation materials makes that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
[4.2.4], the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
[4.3.1], make probiotics leaven with the pasty state fermentation materials, be that masterbatch continuously ferments promptly with the pasty state fermentation materials, the pasty state fermentation materials is inoculated by weight 1:10～25 with granular material to be fermented, with conventional running water preparation liquid state fermentation material, make that moisture is 70～85% in the liquid state fermentation material, or by 20～43% of fermentation base-material dry matter weight, stir, the liquid state fermentation material should be fully immersed in the inoculation bacterium liquid that contains leavening, and its upper strata keeps the inoculation bacterium liquid of height 〉=5.0 centimetres to make air barrier;
[4.3.2], the liquid state fermentation material is put in the fermenting cellar under 23～42 ℃ of temperature, through 20～60 hours standing for fermentation, cultivation, can change the mass fermentation;
[4.3.3], by the liquid state fermentation material with treat that fermentation materials 1:0.45～0.70 is formulated as the pasty state fermentation materials, make that moisture is 52～63% in the pasty state fermentation materials, or by 45～65% of fermentation base-material dry matter weight;
[4.3.4], the pasty state fermentation materials stirred after, the intrapore gas of pasty state fermentation materials is got rid of, compression molding in the chamber of not leaving a blank as far as possible;
[5], adopt by the packaging bag of three layers of bag membrane orienting exhaust, the pasty state fermentation materials that packaging step [4] obtains;
[6], pasty state fermentation materials packing is transported to fermenting cellar after finishing, and is staggered pile, 30～42 ℃ of environment temperatures, 30～50 hours standing for fermentation is got rid of the gas that fermentation produces in the mesopelagic layer bag, with rubber band lock ring mesopelagic layer sack, transports fermenting cellar system ferment;
[7], to the feedstock property of the pasty state fermentation materials after the fermentation according to the fermentation base-material, adopt the method for nature system ferment and vacuum-packed system ferment to stop to ferment respectively,
[7.1], carry out nature system ferment when the fermented feed raw material is thick matter cheap raw material, even it fully ferments, treat that fermentation materials is low to acidity because of fermenting, i.e. PH≤4.0,2～4 day, seal package again when microorganism stops growing;
[7.2], when the fermented feed raw material is concentrated feed, carried out appropriate fermentation, promptly fermented 1～2 day, made microbial growth stop at the later stage of logarithmic growth, guaranteed the quality with vacuum packaging system ferment, vacuum-packed at once after promptly transporting from fermenting cellar;
[8], the pasty state fermented feed behind the seal package is put warehouse storage, special warehouse, normal temperature storage, routine is piled up, environment clean and dry, shady and cool ventilation.
3, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1, it is characterized by: the fermentation base-material basic nutrition content of fermented feed raw material: crude protein 〉=16.0%, lysine 〉=0.7%, egg+cystine 〉=0.5%, threonine 〉=0.5%; Calcium 〉=0.09%; Total phosphorus 〉=0.3%.
4, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1, it is characterized by: the fermentation base-material dry of fermented feed raw material is prepared into by the material component of following weight portion: corn flour 53, bean cake powder 25, ground rice 15, flour 12, bean powder 9, Fructus Hordei Germinatus 6, growth factor 0.5; Moisture 57% in the pasty state fermentation materials, or by fermentation base-material dry matter weight 46%.
5, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 is characterized by: the inoculative proportion of probio bacterial classification is a lactobacillus plantarum 5.5 * 10
6Cfu/ml, saccharomycete 2.5 * 10
6Cfu/ml, bacillus subtilis 2.2 * 10
6Cfu/ml, Bifidobacterium 3.5 * 10
6Cfu/ml.
6, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 is characterized by: when making probiotics leaven with the pasty state fermentation materials, the fermentation base-material must be expanded.
7, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 is characterized by: the pasty state fermentation materials must not continuously ferment above twice as leavening.
8, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 is characterized by: produce the amplification bacterial classification with the pasty state fermentation materials as leavening, change the mass fermentation after bacterial classification is amplified in liquid state fermentation;
Directly make the liquid state fermentation feed with the pasty state fermentation materials as leavening;
Produce wet-milling shape fermented feed, moisture content 20～42% with amplifying again behind the continuous fermentation method production liquid state fermentation material;
Amplify production wet-milling shape fermented feed, moisture content 20～42% with the pasty state fermented feed.
9, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 and 2 is characterized by: the packaging bag of three layers of bag membrane orienting exhaust comprises three layers of inner bags, mesopelagic layer bag, outer membrane bag; Inner bag is an air permeable waterproof formula PE plastic foil, and air permeable waterproof formula PE plastic foil is close to pasty state fermented feed surface, and is seamless packing between the pasty state fermented feed; The mesopelagic layer bag is the food fresh keeping PE plastic foil of waterproof gas formula, and fermentation gas is too fast, or gas production is when excessive, can directly emit the fermentation gas that accumulates in the mesopelagic layer bag and in time seals/tying; The outer membrane bag is an outer packaging bag.
10, the preparation method of the probiotics fermentation activity pasty feedstuff of a kind of general for animal according to claim 1 and 2 is characterized by: the product design of pasty state fermented feed is rolled into square, cuboid, cylinder or sphere at least.
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