CN101446578B - Rapid measurement method of neomycin content in fermentation liquid and device therefor - Google Patents
Rapid measurement method of neomycin content in fermentation liquid and device therefor Download PDFInfo
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- CN101446578B CN101446578B CN2008102374494A CN200810237449A CN101446578B CN 101446578 B CN101446578 B CN 101446578B CN 2008102374494 A CN2008102374494 A CN 2008102374494A CN 200810237449 A CN200810237449 A CN 200810237449A CN 101446578 B CN101446578 B CN 101446578B
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Abstract
The invention relates to a rapid measurement method of neomycin content in fermentation liquid and a device therefor. The method realizes rapid measurement of neomycin content in fermentation liquid by using new technique of separating neomycin from fermentation liquid through ion exchange in combination of optical rotation method. The measurement time is reduced from 20 h spent in the prior measurement method to within 1 h. The fermentation process conditions can be controlled in time by monitoring the neomycin content in the fermentation production in time, so as to provide technical support for improving fermentation production level. The method and device can also be used for research on neomycin fermentation, which can monitor the neomycin content during a shake flask fermentation process in time as well as measure the terminal neomycin content in the shake flask fermentation in time, so as to provide technical support for improving fermentation research level and research efficiency. In addition, the method can not only rapidly measure a single sample, but also can rapidly measure multiple samples at the same time; and the measurement time and cost are both lower than those in the prior art.
Description
Technical field
The present invention relates to neomycin Determination on content method and device in a kind of fermentation liquor, relate in particular to a kind of neomycin in the fermentation liquor is all separated fast after, measure the method and the device of its content with the optical activity method.
Background technology
Neomycin Determination on content method mainly contains antibiotic-microbial assay, high performance liquid chromatography, spectrophotometric method and optical activity method.Antibiotic-microbial assay is that neomycin class finished product is the legal assay method of neomycinsulphate bulk drug and formulation content thereof, see 79 pages of 754 pages in two texts of Chinese Pharmacopoeia version in 2005 and appendix in detail, this method is because specificity is higher, except that the assay that can be used for neomycin class finished product, also can be used for neomycin Determination on content in the fermentation liquor, but because this method complex operation, minute needs 20 hours approximately, this just can not in time learn the content of neomycin in the sweat, thereby also just can not in time regulate and control technological condition for fermentation, this just is unfavorable for improving the fermenting and producing level.High performance liquid chromatography, spectrophotometric method and optical activity method all belong to the Instrument measuring method, though minute all less than 1 hour, Determination on content has a strong impact on because a large amount of impurity in the fermentation liquor can produce serviceable life of instrument and measurement result.And with the neomycin in the fermentation liquor with after a large amount of impurity separate, just can be with above-mentioned Instrument measuring method, problem be prior art to neomycin and a large amount of separate impurities times generally all more than 80 hours, but also not only these methods can only be used for the assay of neomycin class finished product, neomycin in the fermentation liquor all can be separated and can not be directly used in the fermentation liquor neomycin, can lose a part of neomycin, this will cause the untrue of measurement result.Thereby in the existing neomycin fermentation production process, the neomycin Determination on content still must not adopt antibiotic-microbial assay then in the fermentation liquor, does not also find neomycin fast Determination method and device in a kind of fermentation liquor.
Summary of the invention
Purpose of the present invention just provides a kind of content of neomycin in the fermentation production process that makes and can in time monitor, thus neomycin fast Determination method and device in the fermentation liquor of realizing technological condition for fermentation is in time regulated and control
The object of the present invention is achieved like this: neomycin fast Determination method in a kind of fermentation liquor may further comprise the steps:
(1). the preparation of solution to be measured: getting fermentation liquor, with conventional centrifugal settling method the solid content in the fermentation liquor is removed, obtain supernatant, is 0.2～10 micron filtering with microporous membrane with supernatant with the aperture, makes the confession separation solution;
(2). ion-exchange separates: the separation solution that supplies of getting 1～6BV, BV is a times ion exchange resin volume, with on the flow velocity of peristaltic pump control 2～40BV/h from handing over post absorption, wash for pushing up with the water of 1～3BV immediately after intact on the separation solution, BV/h be a times ion exchange resin volume/hour; Subsequently with the ammoniacal liquor desorb of 1～4mol/L in handing over post neomycin and collect stripping liquid with graduated container immediately, the stripping liquid volume of collection is got for the separation solution volume 2～6 times for institute, fully mixes the back as supplying mensuration solution;
(3). the optical activity method is measured: above-mentioned gained is measured its optical activity for measuring solution with 1 decimeter long polarization tube angle-of-rotation measuring method routinely, be calculated as follows the content of neomycin in the fermentation liquor: C
1(mg/ml)=K
1V
2α/V
1
C in the formula
1Be the neomycin weight in every ml fermentation liquor, unit is mg;
K
1Be characteristic coefficient, main relevant with the character of neomycin, value is 12.3～12.9;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml;
Content as if represent neomycin in the fermentation liquor with fermentation unit then is calculated as follows: C
2(μ/ml)=K
2V
2α/V
1
C in the formula
2Be the neomycin unit (μ) in every ml fermentation liquor;
K
2Be characteristic coefficient, main relevant with the character of neomycin, value is 12300～12900;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml.
From the processing of handing over post: with the water of 3～6BV the ammoniacal liquor in the post is washed after most promptly reusable from the friendship post after desorb finishes in step (2); Same after handing over post accumulative total to use more than 10 times, need to handle with the flow velocity of 1～2BV/h with the 2mol/L sodium hydroxide solution of 3～6BV, with after the washing of 3～6BV again the 2mol/L ammoniacal liquor with 3～6BV transfer ion exchange resin to the ammonium type, can drop into next round after washing the ammoniacal liquor in the post to the greatest extent with the water of 3～6BV at last to use.
The described consumption for separation solution of step (2) is 2～4BV; Flow velocity 8～20BV/h that ion-exchange separates; The consumption that the water top is washed is 1.5～2BV; The concentration of described ammoniacal liquor is 2mol/L.
The volume of the described collection stripping liquid of step (2) is 2～4 times for the separation solution volume.
The span of the described characteristic coefficient of step (3) is K
1=12.4～12.6, K
2=12400～12600;
Neomycin fast Determination device in a kind of fermentation liquor, the mainly peristaltic pump 2 that matches with ion exchange column 1 by ion exchange column 1, receiving flask 3 and polarimeter 4 formations.
Ion exchange column 1 column internal diameter scope is 3～24mm, and more excellent column internal diameter scope is 6～12mm; The column length scope is 120～960mm, and more excellent column length scope is 240～480mm; Resin loading amount scope is 0.68～347ml, and more excellent resin loading amount scope is 5.4～43.4ml; The type of the ion exchange resin of adorning is macroporous strong-acid cation-exchange resin and macroporous ion exchange resin, and more excellent type is a macroporous ion exchange resin; The particle size range of ion exchange resin is 0.08～0.64mm, and more excellent particle size range is 0.16～0.32mm; The adsorption capacity scope of ion exchange resin is 2.5～250,000 μ/ml, and more excellent adsorption capacity scope is 10～250,000 μ/ml;
The granularity of ion exchange column 1 intermediate ion exchange resin is 1: 20～80 with ratio from the internal diameter of handing over post;
Ion exchange column 1 internal diameter and length ratio are 1: 20～60;
The volume of ion exchange column 1 is 1: 0.6～1 with the ratio of the loading amount of resin;
Peristaltic pump 2: the flow velocity of peristaltic pump is 0.06～600ml/ minute, and the port number of peristaltic pump is 1～60;
Receiving flask 3: the range of capacity of receiving flask is 5～1000ml, and more excellent range of capacity is 25～100ml;
Polarimeter 4: but the minimum transmitance of test sample product is 1～10%, and more excellent is 1%; The least count value is 0.001～0.01 °, and more excellent is 0.001 °.
8. neomycin fast Determination device in the fermentation liquor according to claim 6 is characterized in that: ion exchange column 1 internal diameter and length ratio are 1: 30～50; Ion exchange column 1 internal diameter and length ratio are 1: 30～50; The volume of ion exchange column 1 is 1: 0.75～0.85 with the ratio of the loading amount of resin.
Adopt the flow velocity from the friendship post of many same sizes of a multi-channel peristaltic pump control, once can measure a plurality of samples simultaneously, once the quantity of while working sample depends primarily on the port number of peristaltic pump.
Neomycin fast Determination method and device in the fermentation liquor provided by the invention, because the new technology that has adopted ion-exchange from fermentation liquor, to separate neomycin fast, and through combining with the optical activity method, realized neomycin fast Determination in the fermentation liquor, minute has dropped in 1 hour by 20 hours that have assay method now, owing to can in time monitor to the content of neomycin in the fermentation production process, just can in time regulate and control, provide technical support for improving the fermenting and producing level to the fermentation manufacturing technique condition.The present invention can also be used for neomycin fermentation research field, both can in time monitor the content of neomycin in the shake flask fermentation process, also can in time measure, provide technical support for improving the fermentation research level and studying efficient to the content of shake flask fermentation terminal point neomycin.In addition, the technology of the present invention can not only be carried out fast measuring to single sample, and can also carry out fast measuring simultaneously to a plurality of samples; The technology of the present invention not only is lower than prior art on minute, and also is lower than prior art on the mensuration expense.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 is a structural representation of the present invention.
Embodiment
Assay method of the present invention may further comprise the steps:
(1). the preparation of solution to be measured: getting fermentation liquor, with conventional centrifugal settling method the solid content in the fermentation liquor is removed, obtain supernatant, is 0.2～10 micron filtering with microporous membrane with supernatant with the aperture, makes the confession separation solution;
(2). ion-exchange separates: the separation solution that supplies of getting 1～6BV, BV is a times ion exchange resin volume, with on the flow velocity of peristaltic pump control 2～40BV/h from handing over post absorption, wash for pushing up with the water of 1～3BV immediately after intact on the separation solution, BV/h be a times ion exchange resin volume/hour; Subsequently with the ammoniacal liquor desorb of 1～4mol/L in handing over post neomycin and collect stripping liquid with graduated container immediately, the stripping liquid volume of collection is got for the separation solution volume 2～6 times for institute, fully mixes the back as supplying mensuration solution;
(3). the optical activity method is measured: above-mentioned gained is measured its optical activity for measuring solution with 1 decimeter long polarization tube angle-of-rotation measuring method routinely, be calculated as follows the content of neomycin in the fermentation liquor: C
1(mg/ml)=K
1V
2α/V
1
C in the formula
1Be the neomycin weight in every ml fermentation liquor, unit is mg;
K
1Be characteristic coefficient, main relevant with the character of neomycin, value is 12.3～12.9;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml;
Content as if represent neomycin in the fermentation liquor with fermentation unit then is calculated as follows: C
2(μ/ml)=K
2V
2α/V
1
C in the formula
2Be the neomycin unit (μ) in every ml fermentation liquor;
K
2Be characteristic coefficient, main relevant with the character of neomycin, value is 12300～12900;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml.
The described consumption for separation solution of step (2) is 2～4BV; Flow velocity 8～20BV/h that ion-exchange separates; The consumption that the water top is washed is 1.5～2BV; The concentration of described ammoniacal liquor is 2mol/L.The volume of the described collection stripping liquid of step (2) is 2～4 times for the separation solution volume.The span of the described characteristic coefficient of step (3) is K
1=12.4～12.6, K
2=12400～12600.
Embodiment 1:
(1). supply the preparation of separation solution: getting the about 100ml of neomycin fermentation liquor that cultivated in 50 tons of fermentation tanks 120 hours, obtain supernatant with conventional centrifugal settling method, is 5 microns filtering with microporous membrane again with the aperture, makes for separation solution.
(2). ion-exchange separates: the dress granularity is the macroporous strong-acid cation-exchange resin 18ml of 0.25～0.35mm in the ion exchange column, the ammonium ion type.Get and supply separation solution 50ml, from handing over post absorption, wash with the water top of 27ml immediately after supplying to finish on the separation solution with Φ 9 * 360 on 4ml/ minute the flow velocity of peristaltic pump control; Also collect stripping liquid to scale with the volumetric flask of 100ml immediately with the neomycin of ammoniacal liquor desorb in handing over post of 2mol/L subsequently, fully mix the back as supplying to measure solution.
(3). the optical activity method is measured: it is 0.435 ° that above-mentioned gained is measured its optical activity for mensuration solution with 1 decimeter long polarization tube angle-of-rotation measuring method (38 pages of two appendix of Chinese Pharmacopoeia version in 2005) routinely, calculates: C
1(mg/ml)=K
1V
2α/V
1=12.6 * 100 * 0.435/50=10.962 (mg/ml);
C
2(μ/ml)＝K
2V
2α/V
1＝12600×100×0.435/50＝10962(μ/ml)。
This example measure from for separation solution to obtain measurement result only the time spent be about 50 minutes, equal measure then with antibiotic-microbial assay that the time spent is 20 hours.
(4). from the processing of handing over post: after desorb finishes in step (2) from handing over post the ammoniacal liquor in the post to be washed most afterwards for use next time with the water of 90ml.
Embodiment 2:
(1). supply the preparation of separation solution: getting the about 60ml of neomycin fermentation liquor that cultivated in 50 tons of fermentation tanks 168 hours, obtain supernatant with conventional centrifugal settling method, is 5 microns filtering with microporous membrane again with the aperture, makes for separation solution.
(2). ion-exchange separates: get for separation solution 25ml, with Φ 7.5 * 300 on 2ml/ minute the flow velocity of peristaltic pump control from handing over post (the macroporous ion exchange resin 10.6ml that interior dress granularity is 0.25～0.35mm, the ammonium ion type) absorption is washed with the water top of 16ml after supplying to finish on the separation solution immediately; Also collect stripping liquid to scale with the volumetric flask of 50ml immediately with the neomycin of ammoniacal liquor desorb in handing over post of 2mol/L subsequently, fully mix the back as supplying to measure solution.
(3). the optical activity method is measured: it is 0.625 ° that above-mentioned gained is measured its optical activity for mensuration solution with 1 decimeter long polarization tube angle-of-rotation measuring method (38 pages of two appendix of Chinese Pharmacopoeia version in 2005) routinely, calculates: C
1(mg/ml)=K
1V
2α/V
1=12.6 * 50 * 0.625/25=15.75 (mg/ml);
C
2(μ/ml)＝K
2V
2α/V
1＝12600×50×0.625/25＝15750(μ/ml)。
This example measure from for separation solution to obtain measurement result only the time spent be about 50 minutes, equal measure then with antibiotic-microbial assay that the time spent is 20 hours.
(4). from the processing of handing over post: after desorb finishes in step (2) from handing over post the ammoniacal liquor in the post to be washed most afterwards for use next time with the water of 50ml.
Embodiment 3:
(1). supply the preparation of separation solution: getting the about 30ml of neomycin fermentation liquor of 500ml shake flask fermentation test gained, obtain supernatant with conventional centrifugal settling method, is 5 microns filtering with microporous membrane again with the aperture, makes for separation solution.
(2). ion-exchange separates: the dress granularity is the macroporous ion exchange resin 4.2ml of 0.18～0.25mm in the ion exchange column, the ammonium ion type.Get and supply separation solution 10ml, from handing over post absorption, wash with the water top of 6ml immediately after supplying to finish on the separation solution with Φ 5.5 * 220 on 1ml/ minute the flow velocity of peristaltic pump control; Also collect stripping liquid to scale with the volumetric flask of 25ml immediately with the neomycin of ammoniacal liquor desorb in handing over post of 2mol/L subsequently, fully mix the back as supplying to measure solution.
(3). the optical activity method is measured: it is 0.520 ° that above-mentioned gained is measured its optical activity for mensuration solution with 1 decimeter long polarization tube angle-of-rotation measuring method (38 pages of two appendix of Chinese Pharmacopoeia version in 2005) routinely, calculates: C
1(mg/ml)=K
1V
2α/V
1=12.6 * 25 * 0.520/10=16.38 (mg/ml);
C
2(μ/ml)＝K
2V
2α/V
1＝12600×25×0.520/10＝16380(μ/ml)。
This example measure from for separation solution to obtain measurement result only the time spent be about 50 minutes, equal measure then with antibiotic-microbial assay that the time spent is 20 hours.
(4). from the processing of handing over post: after desorb finishes in step (2) from handing over post the ammoniacal liquor in the post to be washed most afterwards for use next time with the water of 20ml.
Ion exchange column after desorb finishes in the step among the present invention (2) is washed after most ammoniacal liquor in the post promptly reusable with the water of 3～6BV; Same after handing over post accumulative total to use more than 10 times, need to handle with the flow velocity of 1～2BV/h with the 2mol/L sodium hydroxide solution of 3～6BV, with after the washing of 3～6BV again the 2mol/L ammoniacal liquor with 3～6BV transfer ion exchange resin to the ammonium type, can drop into next round after washing the ammoniacal liquor in the post to the greatest extent with the water of 3～6BV at last to use.
Neomycin fast Determination device in the fermentation liquor among the present invention, mainly by ion exchange column 1, the peristaltic pump 2 that matches with ion exchange column 1, receiving flask 3 and polarimeter 4 constitute, and feed in raw material by charge pipe 5.
Ion exchange column 1 column internal diameter scope among the present invention is 3～24mm, and more excellent column internal diameter scope is 6～12mm; The column length scope is 120～960mm, and more excellent column length scope is 240～480mm; Resin loading amount scope is 0.68～347ml, and more excellent resin loading amount scope is 5.4～43.4ml; The type of the ion exchange resin of adorning is macroporous strong-acid cation-exchange resin and macroporous ion exchange resin, and more excellent type is a macroporous ion exchange resin; The particle size range of ion exchange resin is 0.08～0.64mm, and more excellent particle size range is 0.16～0.32mm; The adsorption capacity scope of ion exchange resin is 2.5～250,000 μ/ml, and more excellent adsorption capacity scope is 10～250,000 μ/ml;
The granularity of ion exchange column 1 intermediate ion exchange resin is 1: 20～80 with ratio from the internal diameter of handing over post;
Ion exchange column 1 internal diameter and length ratio are 1: 20～60;
The volume of ion exchange column 1 is 1: 0.6～1 with the ratio of the loading amount of resin;
Peristaltic pump 2: the flow velocity of peristaltic pump is 0.06～600ml/ minute, and the port number of peristaltic pump is 1～60;
Receiving flask 3: the range of capacity of receiving flask is 5～1000ml, and more excellent range of capacity is 25～100ml;
Polarimeter 4: but the minimum transmitance of test sample product is 1～10%, and more excellent is 1%; The least count value is 0.001～0.01 °, and more excellent is 0.001 °.
Ion exchange column 1 internal diameter and length ratio are 1: 30～50; Ion exchange column 1 internal diameter and length ratio are 1: 30～50; The volume of ion exchange column 1 is 1: 0.75～0.85 with the ratio of the loading amount of resin.
Control the flow velocity from the friendship post of many same sizes among the present invention as if 2 pumps of wriggling with a hyperchannel, then once can measure a plurality of samples simultaneously, once the quantity of while working sample depends primarily on the port number of peristaltic pump, commercially available peristaltic pump port number has reached more than 30 passages at present, reached 36 passages at most, this measures very rapidly and efficiently for the neomycin fermentation unit that carries out extensive shake flask fermentation test.
Claims (8)
1. neomycin fast Determination method in the fermentation liquor is characterized in that: may further comprise the steps:
(1). the preparation of solution to be measured: getting fermentation liquor, with conventional centrifugal settling method the solid content in the fermentation liquor is removed, obtain supernatant, is 0.2 ~ 10 micron filtering with microporous membrane with supernatant with the aperture, makes the confession separation solution;
(2). ion-exchange separates: the separation solution that supplies of getting 1 ~ 6BV, BV is a times ion exchange resin volume, with ion exchange column absorption on the flow velocity of peristaltic pump control 2 ~ 40BV/h, wash for the water top of using 1 ~ 3BV after intact on the separation solution immediately, BV/h be a times ion exchange resin volume/hour; Use the neomycin in the ammoniacal liquor maldi ion exchange column of 1 ~ 4mol/L subsequently and collect stripping liquid with graduated container immediately, the stripping liquid volume of collection is got for the separation solution volume 2 ~ 6 times for institute, fully mixes the back as supplying mensuration solution;
(3). the optical activity method is measured: above-mentioned gained is measured its optical activity for measuring solution with 1 decimeter long polarization tube angle-of-rotation measuring method routinely, be calculated as follows the content of neomycin in the fermentation liquor: C
1(mg/ml)=K
1V
2α/V
1
C in the formula
1Be the neomycin weight in every ml fermentation liquor, unit of weight is mg;
K
1Be characteristic coefficient, main relevant with the character of neomycin, value is 12.3 ~ 12.9;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml;
Content as if represent neomycin in the fermentation liquor with fermentation unit then is calculated as follows: C
2(μ g/ml)=K
2V
2α/V
1
C in the formula
2Be the neomycin unit in every ml fermentation liquor (μ g);
K
2Be characteristic coefficient, main relevant with the character of neomycin, value is 12300 ~ 12900;
Accurately numerical value can compare to measure and try to achieve with antibiotic-microbial assay;
α is the optical activity that records;
V
1Be the volume of getting for separation solution, unit is ml;
V
2Be the volume for mensuration solution, unit is ml.
2. neomycin fast Determination method in a kind of fermentation liquor according to claim 1 is characterized in that: the processing of ion exchange column: the ion exchange column after desorb finishes in step (2) is washed after most ammoniacal liquor in the post promptly reusable with the water of 3 ~ 6BV; After same radical ion exchange column accumulative total is used more than 10 times, need to handle with the flow velocity of 1 ~ 2BV/h with the 2mol/L sodium hydroxide solution of 3 ~ 6BV, transfer ion exchange resin to the ammonium type with the 2mol/L ammoniacal liquor of using 3 ~ 6BV after the washing of 3 ~ 6BV again, can drop into next round after the water of using 3 ~ 6BV is at last washed the ammoniacal liquor in the post to the greatest extent and use.
3. neomycin fast Determination method in the fermentation liquor according to claim 1 is characterized in that: the described consumption for separation solution of step (2) is 2 ~ 4BV; Flow velocity 8 ~ 20BV/h that ion-exchange separates; The consumption that the water top is washed is 1.5 ~ 2BV; The concentration of described ammoniacal liquor is 2mol/L.
4. neomycin fast Determination method in the fermentation liquor according to claim 1 is characterized in that: the volume of the described collection stripping liquid of step (2) is 2 ~ 4 times for the separation solution volume.
5. neomycin fast Determination method in the fermentation liquor according to claim 1 is characterized in that: the span of the described characteristic coefficient of step (3) is K
1=12.4 ~ 12.6, K
2=12400 ~ 12600.
6. neomycin fast Determination device in the fermentation liquor, it is characterized in that: mainly by ion exchange column (1), the peristaltic pump (2) that matches with ion exchange column (1), receiving flask (3) and polarimeter (4) constitute, and ion exchange column (1) column internal diameter scope is 3 ~ 24 mm; The column length scope is 120 ~ 960 mm; Resin loading amount scope is 0.68 ~ 347 ml; The type of the ion exchange resin of adorning is macroporous strong-acid cation-exchange resin and macroporous ion exchange resin; The particle size range of ion exchange resin is 0.08 ~ 0.64mm; The adsorption capacity scope of ion exchange resin is 2.5 ~ 250,000 μ g/ml;
The granularity of ion exchange column (1) intermediate ion exchange resin is 1: 20 ~ 80 with the ratio of the internal diameter of ion exchange column;
Ion exchange column (1) internal diameter and length ratio are 1: 20 ~ 60;
The volume of ion exchange column (1) is 1: 0.6 ~ 1 with the ratio of the loading amount of resin;
Peristaltic pump (2): the flow velocity of peristaltic pump is 0.06 ~ 600 ml/ minute, and the port number of peristaltic pump is 1 ~ 60;
Receiving flask (3): the range of capacity of receiving flask is 5 ~ 1000ml;
Polarimeter (4): but the minimum transmitance of test sample product is 1 ~ 10%; The least count value is 0.001 ~ 0.01 °.
7. neomycin fast Determination device in the fermentation liquor according to claim 6 is characterized in that: ion exchange column (1) internal diameter and length ratio are 1: 30 ~ 50; The volume of ion exchange column (1) is 1:0.75 ~ 0.85 with the ratio of the loading amount of resin.
8. neomycin fast Determination device in the fermentation liquor according to claim 6, it is characterized in that: the flow velocity that adopts the ion exchange column of many same sizes of a multi-channel peristaltic pump control, once can measure a plurality of samples simultaneously, once the quantity of while working sample depends primarily on the port number of peristaltic pump.
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| CN104558068B (en) * | 2014-12-19 | 2017-06-06 | 宜昌三峡制药有限公司 | A kind of method and equipment therefor for extracting high-quality neomycin |
| CN108226348A (en) * | 2018-03-20 | 2018-06-29 | 广西壮族自治区食品药品检验所 | Neomycinsulphate component and the HPLC detection methods in relation to substance |
| CN110872333A (en) * | 2019-11-25 | 2020-03-10 | 宜昌三峡制药有限公司 | Process control method for neomycin preparation process |
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