CN101424693A - Portable plumbum ion concentration analyzer using disposable sensor - Google Patents
Portable plumbum ion concentration analyzer using disposable sensor Download PDFInfo
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- CN101424693A CN101424693A CN 200810235364 CN200810235364A CN101424693A CN 101424693 A CN101424693 A CN 101424693A CN 200810235364 CN200810235364 CN 200810235364 CN 200810235364 A CN200810235364 A CN 200810235364A CN 101424693 A CN101424693 A CN 101424693A
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Abstract
The invention relates to a portable lead ion concentration analysis meter with a disposable sensor, which is used for the detection of lead ion concentration in blood or urine. The analysis meter comprises a detecting instrument, a disposable sensor and a correction card, wherein the detecting instrument is provided with a sensor interface, a correction card interface, a display screen, buttons, and a communication serial port which needs to be connected with a computer; the disposable sensor comprises a substrate, working electrodes, auxiliary electrodes, reference electrodes, insulating layers and covering resins, the working electrodes, the auxiliary electrodes and the reference electrodes are positioned on the substrate, and the covering resins are positioned on the insulating layers positioned on the electrodes; and the correction card is used for correcting parameters detected by the detecting instrument. The invention has the advantages that the analysis meter can automatically complete the processes of parameter setting, collection, dissolution, data collection, background correction, characteristic potential judgment, concentration calculation, sensor correction, result display, and the like which are needed by the entire detection, thus the analysis meter avoids the error caused by human elements during the detection process of the traditional electrochemical method; and besides, the analysis meter has cabinet configuration and low cost and is suitable for basic medical institutions.
Description
Technical field
The present invention relates to a kind of portable plumbum ion concentration analyzer that uses disposable sensor, the lead ion content that is used for blood or urine detects.
Background technology
Lead is a kind of human body to be had neurovirulent heavy metal element, and children's nervous system is in the process of improving of growing fast, this in period nervous system the resistivity of toxicant is the most fragile to external world.Domestic and international research has been found that all the incidence of disease of children mental retardation is high more in the serious more place of environment lead contamination.The every rising 10g/dl of children's Pb-B, its IQ (IQ) will descend 6～8 fens.The Dai Yaohua researcher of Shoudu Inst. of Pediatrics etc. studies show that, Chinese city children ' s lead poisoning rate is 10.45%, the Pb-B population mean is 59.52 μ g/L, Pb-B 〉=100 μ g/L persons account for 10.45%, Pb-B 〉=200 μ g/L persons only are 0.62%, previously the epidemiological study result is existing descends, but still is higher than developed country's lead levels.
Atomic absorption spectrography (AAS), chemical colorimetry are arranged, the anode leaching for the present domestic common detection method of the detection of blood lead.The principle of work that atomic absorption spectrum detects: make sample atomization in the tungsten boat by electrical heating, produce a large amount of ground state free atoms, thereby absorb the characteristic spectral line of the tested element of launching by hollow cathode lamp, finish measuring process.This method needs large-scale Atomic Absorption Spectrometer and special-purpose gas, is detection method commonly used in the specialized laboratory.This method instrument costs an arm and a leg, and is not suitable for outdoor scene and detects.Chemical colorimetry is to use the lead reaction in dithizone and the sample, and with the formed complex compound of chloroform extraction and carry out colorimetric detection, the shortcoming of this method is that disturbing factor is many, and personal error is bigger.The anode leaching is because method is simple, and highly sensitive development in recent years is quick, and the blood lead detecting instrument of stripping class is a lot of on the market.Because adopt a mercury worktable or quiet mercury worktable collocation stripping voltamograph as detection platform, sample needs digestion process, aspects such as the maintenance correction of instrument are more complicated also.
The ubiquity that detects demand owing to blood lead has determined that grass-roots community Health Services center is the suitable mechanism that carries out this detection, and reality is that the universal situation of blood lead detection at grass-roots community Health Services center is very low at present because the price barrier of the outfit digestion instrument at grass-roots community Health Services center and Atomic Absorption Spectrometer and the operational complexity of chemical colorimetric method cause.Therefore need a kind of method of operating simple, low, quick, the portable blood lead detecting instrument of cost.
Summary of the invention
The objective of the invention is for the lead ion in basic hospital mechanism or outdoor whole blood, serum or the urine detect provide a kind of quick, single part operate, be easy to carry, the portable plumbum ion concentration analyzer of low cost and use disposable sensor simple to operate.
According to technical scheme provided by the invention, use the portable plumbum ion concentration analyzer of disposable sensor, comprise detecting instrument, disposable sensor and correction card, it is characterized in that: described detecting instrument is used to detect parameters needed setting, enrichment, stripping, data acquisition, background deduction, the judgement of feature current potential, concentration calculating, sensor calibration and result and shows; Described detecting instrument has sensor interface, the correction card interface that connects correction card that connects disposable sensor, communication serial ports, display screen and the button that connects computing machine; Described disposable sensor comprises substrate, be positioned at on-chip working electrode, auxiliary electrode and contrast electrode, be positioned at the insulation course on the electrode and be positioned at covering resin on the insulation course; Described correction card and disposable sensor and the specific supporting use of sample preparation liquid are used for the detected parameter of correct detection instrument.
The working electrode of described disposable sensor and auxiliary electrode are carbon electrode, and described contrast electrode is the Ag/AgCl electrode, and described substrate is the square sheet of PET material; The shape of described three electrodes is strip.(Ag/AgCl is meant silver or silver chloride).
Described insulation course covers described electrode and reserves the area that exposes of electrode.
Described covering resin covers insulation course and forms and detects the hole, and described detection hole is oval cavity, is used to hold the detected sample that is dripped when detecting.
Described correction card is a wire-jumper type inserted sheet, and built-in 5 groups of wire jumpers are used to produce 16 kinds of different correction parameters, to proofread and correct and the supporting disposable sensor of this correction card and the detected parameters of sample preparation liquid.
The work of described detecting instrument is by being positioned at its inner Single-chip Controlling.
The end of described Ag/AgCl electrode is divided into two branches, and when it inserted described sensor interface, described detecting instrument detected whether inserted electrode.
Advantage of the present invention is: adopt under the Single-chip Controlling and finish processes such as the setting of whole detection parameters needed, enrichment, stripping, data acquisition, background deduction, the judgement of feature current potential, concentration calculating, sensor calibration, demonstration as a result automatically, avoided the error of human factor in the traditional electrical chemical method testing process, apparatus structure is succinct, profile is small and exquisite, with low cost, be suitable for basic medical unit and use.
Description of drawings
Fig. 1 structural drawing of the present invention.
Fig. 2 is a detecting instrument mimic channel synoptic diagram
Fig. 3 is the structural drawing of disposable sensor.
Fig. 4 is the correction card outside drawing.
Fig. 5 is an electrode pre-oxidation current potential sequential chart.
Fig. 6 is that disposable sensor collocation electrochemical analyser uses figure as a result.
Embodiment
The invention will be further described below in conjunction with drawings and Examples.
The invention provides one and plumbum ion concentration in whole blood, serum, urine or the water sample detected comprised, from the full-automatic solution of all processes such as sample disposal, sensor calibration.The technology that the present invention relates to comprises current potential dissolving-out technology, disposable plumbous sensor technology, interior scalar quantity technology.
Detecting instrument of the present invention adopts full-automatic differetial-potential stripping analysis method, adopt under the Single-chip Controlling on the detecting instrument structure and finish processes such as the setting of whole detection parameters needed, enrichment, stripping, data acquisition, background deduction, the judgement of feature current potential, concentration calculating, sensor calibration, demonstration as a result automatically, avoided the error of human factor in the traditional electrical chemical method testing process.
As shown in Figure 1, the present invention includes detecting instrument 12, disposable sensor 10 and correction card 13, described detecting instrument 12 has sensor interface 14, the correction card interface 17 that connects correction card that connects disposable sensor, communication serial ports 16, display screen 15 and the button 18 that connects computing machine.
Described disposable sensor 10 is disposable jettisonable printed sensor, it is a three-electrode system, comprise two carbon electrodes and an Ag/AgCl electrode, adopted the preoxidation technique of screen printing technique (or thick film screen printing technology) and carbon electrode, the application of disposable sensor 10 makes the detection of plumbum ion concentration can break away from necessary mercury of traditional digestion analyzer or quiet mercury worktable, make the transportability of instrument strengthen greatly, can carry out the scene out of doors detects, and realized the single sample fast detecting of blood lead, avoided that single sample detects uneconomic problem in traditional stripping analysis, make the dirigibility on detection opportunity increase greatly, the timeliness of providing examining report improves greatly.
Described disposable sensor 10 comprises substrate, be positioned at on-chip working electrode, auxiliary electrode and contrast electrode, be positioned at the insulation course on the electrode and be positioned at covering resin on the insulation course.Working electrode and auxiliary electrode are carbon electrode, and contrast electrode is the Ag/AgCl electrode, and described substrate is the square sheet of PET (polyethylene terephthalate) material; The shape of described three electrodes is strip.
As shown in Figure 3,9 is two carbon electrodes, and 8 is the Ag/AgCl electrode, and serigraphy (or thick film screen printing) forms, and 6 is resin bed, and 7 is insulation course.The part that Ag/AgCl electrode 8 inserts sensor interface is divided into two-way branch, is used for detecting instrument and detects whether inserted electrode.Insulating oil is used for fixing electrode and exposes area, and resin is used to form and detects the hole, and the area of each electrode can influence the precision of detection and the consistance between different sensors.Adopt the inert plastic material to cover and form and detect needed oval cavity, this cavity is the sample to be detected that is dripped when detecting in order to hold.
Described disposable sensor 10 adopts screen printing technique (or thick film screen printing technology) to be made, at first use the drafting instrument of high precision such as CAD to produce needed electrode shape and tectal shape, make the film (egative film) back and on 200～400 purpose silk screen versions, carry out printing down, the formation needed half tone of biting prints carbon electrode, Ag/AgCl electrode, insulation course, covering resin successively on the PET substrate.
Described disposable sensor 10 has also adopted preoxidation technique, can not use preplating mercury film or coordination plating mercury technology when its collocation electrochemical apparatus is used, and need not like this to add mercury ion in sample preparation liquid, effectively reduces the pollution of mercury to environment.Preoxidation technique applies a very high voltage to the carbon working electrode exactly, and (=C-O) increases, and increases the accumulation ability of heavy metal at electrode surface, and this sensor is inserted the galvanochemistry worktable, places 0.1M H to make the carbon oxygen functional group group of electrode surface
2SO
4In, the current potential that working electrode is set is 2.2V, continues 10～20 seconds, carries out the working electrode that scan round can obtain pre-oxidation for 15～50 times from-1V～1V again.Electrode pre-oxidation current potential sequential chart as shown in Figure 5.
The present invention adopts sample preparation liquid that sample is handled, and provides to detect required pH value, ionic strength, internal standard compound matter.The standard addition method of traditional stripping analysis has been abandoned in the adding of internal standard compound matter, thereby has realized directly utilizing in an enrichment, process in leaching the reference of internal standard compound matter to calculate the concentration of blood lead.The introducing of internal standard compound matter is to realize disposable sensor 10 key in application in plumbum ion concentration detects, adopting mark-on graphing method (being called for short the mark-on method) or typical curve relative method traditionally is inappropriate for the application of disposable sensor 10, because disposable sensor more than 10 is generated by serigraphy or thick film screen printing technology, the surface condition complexity of working electrode, the relative also more complicated with the situation of stripping of the enrichment of ion, enrichment, the stripping situation behind each mark-on of can't guaranteeing in the mark-on method is consistent and cause the inaccurate of testing result.A kind of scheme of sample preparation liquid is: 0.1～1M HNO
3, 0.2M KCl is an end liquid, the thallium ion that does not have in the internal standard compound matter selection human body, and final concentration is 100 μ g/l.
As shown in Figure 4, described correction card is a wire-jumper type inserted sheet, and built-in 5 groups of wire jumpers can be used to produce 16 kinds of different correction parameters, are used to proofread and correct the detected parameters of each batch sensor and sample preparation liquid, guarantees the consistance of testing result.1 pin connects the single-chip microcomputer high level, and 2 to 5 pin connect the data pin of single-chip microcomputer respectively, and it is " 1 " that described data pin is connected high level, is broken as " 0 ", totally 16 kinds of states.
As shown in Figure 1, during use,
1, sample to be detected is joined mixing in the sample processing tube.
2, open detecting instrument, insert correction card and the disposable sensor 10 identical with disposable sensor 10 lot numbers.
3, sample of the fluid drips in the sample processing tube is to the sensor hole.
4, detect by START button.
5, the result shows.
Be illustrated in figure 2 as the part testing circuit of detecting instrument, comprise constant potential controller U5-A, anti-self-excitation capacitance C7, impedance transformer U8, optional current amplifier U5-B, resistance R 14.By switch S 1, S2, S3, S4 and wire jumper tie point 1,2,3 control circuit structures.Node C connects the auxiliary electrode of disposable sensor 10, and node W connects the working electrode of disposable sensor 10, and node R connects the contrast electrode of disposable sensor 10.
During holding state: switch S 1, S4 close, protection impedance transformer U8, constant potential controller U5-A, optional current amplifier U5-B.
During enrichment state: switch S 1, S4 open, and switch S 2, S3 close; Provide accumulating potential by D/A (digital to analog converter), delay time by Single-chip Controlling.
During the stripping state: switch S 1 is closed, switch S 2, S3, S4 open, and D/A ends output, and resistance R 14 terminal potentials send A/D (analog to digital converter) to gather after by impedance transformer U8 impedance conversion, data are analyzed by single-chip microcomputer after finishing, and the result send display screen to show.
Claims (7)
1, a kind of portable plumbum ion concentration analyzer that uses disposable sensor comprises detecting instrument, disposable sensor and correction card, it is characterized in that:
Described detecting instrument is used to detect parameters needed setting, enrichment, stripping, data acquisition, background deduction, the judgement of feature current potential, concentration calculating, sensor calibration, Presentation Function as a result; Described detecting instrument has sensor interface, the correction card interface that connects correction card that connects disposable sensor, communication serial ports, display screen and the button that connects computing machine;
Described disposable sensor comprises substrate, be positioned at on-chip working electrode, auxiliary electrode and contrast electrode, be positioned at the insulation course on the electrode and be positioned at covering resin on the insulation course;
Described correction card and disposable sensor and the specific supporting use of sample preparation liquid are used for the detected parameter of correct detection instrument.
2, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1, it is characterized in that: the working electrode of described disposable sensor and auxiliary electrode are carbon electrode, described contrast electrode is the Ag/AgCl electrode, and described substrate is the square sheet of PET material; The shape of described three electrodes is strip.
3, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1 is characterized in that: described insulation course covers described electrode and reserves the area that exposes of electrode.
4, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1, it is characterized in that: described covering resin covers insulation course and forms and detects the hole, described detection hole is oval cavity, is used to hold the detected sample that is dripped when detecting.
5, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1, it is characterized in that: described correction card is a wire-jumper type inserted sheet, built-in 5 groups of wire jumpers, be used to produce 16 kinds of different correction parameters, to proofread and correct and the supporting disposable sensor of this correction card and the detected parameters of sample preparation liquid.
6, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1 is characterized in that: the work of described detecting instrument is by being positioned at its inner Single-chip Controlling.
7, the portable plumbum ion concentration analyzer of use disposable sensor as claimed in claim 1, it is characterized in that: the end of described Ag/AgCl electrode is divided into two branches, when it inserted described sensor interface, described detecting instrument detected whether inserted electrode.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043039A (en) * | 2009-10-21 | 2011-05-04 | 合世生医科技股份有限公司 | Method for setting instrument parameters |
| CN103149255A (en) * | 2013-02-25 | 2013-06-12 | 长沙理工大学 | Method for quickly determining lead content in blood |
| CN103207223A (en) * | 2012-11-23 | 2013-07-17 | 上海仪电科学仪器股份有限公司 | Manufacturing method of portable heavy metal meter printing electrode |
| CN104833711A (en) * | 2015-03-09 | 2015-08-12 | 上海伯顿医疗设备有限公司 | Blood lead analysis instrument and blood lead measuring method therewith |
| CN105424916A (en) * | 2015-12-09 | 2016-03-23 | 桂林优利特医疗电子有限公司 | Electrode codes of biosensor test paper, recognition device and recognition method |
| CN115201289A (en) * | 2021-04-12 | 2022-10-18 | 广东朗源生物科技有限公司 | Heavy metal detection method based on screen printing electrode and detection instrument thereof |
Family Cites Families (4)
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| CN1187607C (en) * | 2002-06-10 | 2005-02-02 | 武汉大学 | Electrochemical sensor and its prepn and use |
| CN101055262A (en) * | 2006-04-11 | 2007-10-17 | 禅谱科技股份有限公司 | Discard type electrochemical sensing test piece and its making process |
| CN101017149A (en) * | 2007-03-05 | 2007-08-15 | 浙江大学 | Disposable blood lead biosensor |
| CN201060198Y (en) * | 2007-06-07 | 2008-05-14 | 复旦大学 | Integrated forming disposable electrochemical sensor for determining blood lead concentration |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102043039A (en) * | 2009-10-21 | 2011-05-04 | 合世生医科技股份有限公司 | Method for setting instrument parameters |
| CN103207223A (en) * | 2012-11-23 | 2013-07-17 | 上海仪电科学仪器股份有限公司 | Manufacturing method of portable heavy metal meter printing electrode |
| CN103207223B (en) * | 2012-11-23 | 2015-04-01 | 上海仪电科学仪器股份有限公司 | Manufacturing method of portable heavy metal meter printing electrode |
| CN103149255A (en) * | 2013-02-25 | 2013-06-12 | 长沙理工大学 | Method for quickly determining lead content in blood |
| CN104833711A (en) * | 2015-03-09 | 2015-08-12 | 上海伯顿医疗设备有限公司 | Blood lead analysis instrument and blood lead measuring method therewith |
| CN104833711B (en) * | 2015-03-09 | 2019-06-25 | 上海伯顿医疗设备有限公司 | A kind of blood lead analyzer and its measurement blood lead method |
| CN105424916A (en) * | 2015-12-09 | 2016-03-23 | 桂林优利特医疗电子有限公司 | Electrode codes of biosensor test paper, recognition device and recognition method |
| CN115201289A (en) * | 2021-04-12 | 2022-10-18 | 广东朗源生物科技有限公司 | Heavy metal detection method based on screen printing electrode and detection instrument thereof |
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Application publication date: 20090506 Assignee: Wuxi Shenrui Bio-Pharmaceuticals Co., Ltd. Assignor: Sheng Qingsong|Zhang Tongjun Contract record no.: 2013320000046 Denomination of invention: Portable plumbum ion concentration analyzer using disposable sensor Granted publication date: 20121226 License type: Exclusive License Record date: 20130220 |
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Effective date of registration: 20201231 Address after: 5 / F, c30303, C5, area C, Wuxi Huishan Life Science Park, 1699 Huishan Avenue, Huishan District, Wuxi City, Jiangsu Province Patentee after: WUXI SHENRUI BIO-PHARMACEUTICALS Co.,Ltd. Address before: 214058 Shen Rui Biological Products Co., Ltd., Huang Xiang hospital, 118 Fengxiang Road, Beitang District, Wuxi, Jiangsu Patentee before: Sheng Qingsong Patentee before: Zhang Tongjun |
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