CN101411721A - Method for preparing fresh pilose antler active cell extract freeze-dried powder - Google Patents
Method for preparing fresh pilose antler active cell extract freeze-dried powder Download PDFInfo
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- CN101411721A CN101411721A CNA2008101801389A CN200810180138A CN101411721A CN 101411721 A CN101411721 A CN 101411721A CN A2008101801389 A CNA2008101801389 A CN A2008101801389A CN 200810180138 A CN200810180138 A CN 200810180138A CN 101411721 A CN101411721 A CN 101411721A
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Abstract
The invention discloses a method for preparing freeze-dried powder of an active cell extract of fresh pilose antler. The preparation method comprises the following steps: fresh and frozen pilose antler is taken out, is sliced, is ground, is added with ethanol, is evenly stirred, is subjected to breaking treatment through an ultrasonic breaking instrument, and is centrifugated to obtain two parts of supernatant liquid and sediment; the part of the supernatant liqid is decompressed, condensed, frozen and dried through a freezedryer to prepare dry powder; and the sediment part is added with the ethanol, is heated and is subjected to ultrasonic breaking treatment and centrifugation. Through the three steps, a finished product can be obtained. The method uses the fresh pilose antler as a raw material, has stage extraction of low temperature and high temperature, ensures that enzyme and other biological active substances are not deactivated in the extraction process and simultaneously ensures the full extraction of other effective compositions. Through detection, the prepared product through the method has stable and high activity of SOD, which shows that the active substances in the cell are well stored and the effective compositions have high contents.
Description
Technical field
The present invention relates to a kind of preparation method of cell extract freeze-dried powder, refer in particular to the preparation method of fresh pilose antler active cell extract freeze-dried powder.
Background technology
Cornu Cervi Pantotrichum is precious Chinese crude drug, simultaneously, is widely used in health product, food, the cosmetic industry as a kind of tonic food again.Known nutritional labeling mainly contains polypeptides matter, ucleotides material, fatty acid material and some have the enzyme and the hormonal substance of active function in the Cornu Cervi Pantotrichum.At present, traditional Cornu Cervi Pantotrichum concocting method is after bright fine and soft receipts are adopted, to boil with boiling water both at home and abroad, and is then, through 70 ℃ of bakings of infrared oven, air-dry again.In this process, the Sanguis cervi loss is more.Simultaneously, because of action of high temperature, the active ingredient loss in the Cornu Cervi Pantotrichum is also very big.And in the young pilose antler after the process of preparing Chinese medicine, the activity of superoxide dismutase (SOD) and alkali phosphatase (AKP) descends significantly.In addition, press traditional extraction technique, steep or organic solvent extraction with steeping in wine Cornu Cervi Pantotrichum section back, because the microporous structure of Cornu Cervi Pantotrichum, the lixiviate required time is very long, simultaneously, also can pollute because of organic solvent residue.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of preparation method of fresh pilose antler active cell extract freeze-dried powder, this preparation method is a raw material with fresh Cornu Cervi Pantotrichum, low temperature, high temperature process combine, and adopt the effective ingredient in the ultrasonic technology extraction pilose antler active cell.Product is fit to be applied to the production of food, health product.At low temperatures after supersound extraction, though it is many that active matter keeps, but, the fat-soluble nutrient substance in the Cornu Cervi Pantotrichum and the extraction of polypeptides matter are on the low side, simple extract at low temperature is inadequate to the extraction of nutritional labeling, outstanding behaviours is that the peptide material extraction ratio is low, and peptide material is considered to one of important component of Cornu Cervi Pantotrichum at present.At this situation, this method has adopted the different temperatures stage to extract.The residue that promptly low thermophase extracts passes through high temperature extraction again, has effectively improved the content of polypeptide class, and the extract with different temperatures merges then.Like this, both guarantee the existence of active substance, nutrient substance is extracted fully, taken into account the extraction of enzyme material and polypeptide.
Technical problem of the present invention is solved by following scheme: the preparation method of fresh pilose antler active cell extract freeze-dried powder, it is characterized in that: get aquatic foods and freeze Cornu Cervi Pantotrichum and be cut into the thick fine and soft sheet of 1.5～2.5mm, ground 15～25 minutes with colloid mill, add 5 times of weight concentrations and be 50% ethanol, stir, through ultrasonic disruption instrument break process 15～25 minutes, got supernatant and precipitation two parts in centrifugal 30 minutes with 5000rpm then, with supernatant part concentrating under reduced pressure, temperature is less than 30 ℃, be concentrated into volume and be original 1/5 (aquatic foods freeze Cornu Cervi Pantotrichum add 5 times of weight ethanol volumes 1/5), freezing after, be dried to dry powder through freeze dryer; It is 50% ethanol that precipitation part is added 5 times of weight concentrations, be heated to 60 ℃, ultrasonic disruption was handled 15～25 minutes, with 5000rpm centrifugal 30 minutes, supernatant and precipitation two parts again were again with this supernatant part concentrating under reduced pressure, temperature is less than 30 ℃, be concentrated into 1/5 (precipitation part add 5 times of weight ethanol volumes 1/5) of original volume, freezing after, be dried to dry powder through freeze dryer; And this precipitation part adds the distilled water of 5 times of weight again, boiling reflux 30 minutes, with 5000rpm centrifugal 30 minutes, precipitation is discarded, and with supernatant part concentrating under reduced pressure, temperature is 60 ℃, be concentrated into 1/2 (precipitation part add 5 times of weight distilled water volumes 1/2) of original volume, be dried to dry powder through the drying under reduced pressure case for 60 ℃ with temperature, last, be finished product after the power-product mix homogeneously packing that above three steps are obtained.
Advantage of the present invention is: 1, this method changes traditional to be raw material to concoct young pilose antler, to be raw material and adopt fresh Cornu Cervi Pantotrichum, and freezing preservation extract at low temperature makes that material such as SOD, AKP can keep active effectively in the extract.Research does not relatively detect the activity of enzyme in the dried young pilose antler after the process of preparing Chinese medicine and the Cornu Cervi Pantotrichum element of import by experiment, and in the product that this method is produced, through check repeatedly, it is stable that the activity of SOD keeps, and active higher, intracellular active substance preservation is described better, the active constituent content height.
2, adopt ultrasonic wave extraction, improved extraction ratio, accelerate extraction rate, help modernization industry and produce.This compares with traditional leach extraction method, and outstanding advantage is arranged.General stirring is extracted, and the needed time is long.In the stirring of routine, after reaching capacity, mixing rate and mass transfer rate become constant, and at this moment, accelerate mixing speed and can not raise the efficiency.And ultrasound wave can play dispersion, pulverizing, multiple-effect.Disperse sized particles, destroy the solvent structure, its dimension is further reduced, improve extraction ratio.
Though 3, cryogenic conditions extracts the activity that can keep materials such as enzyme effectively down.But, consider Cornu Cervi Pantotrichum as a kind of tonic food, its effective ingredient is very complicated.It is not only the effect of compositions such as enzyme, glutathion.Wherein the effect of hormones and polypeptides matter has had a lot of reports, separates from Cornu Cervi Pantotrichum at present as the antiinflammatory polypeptide, and with proof good antiinflammatory action is arranged.This class polypeptide thing extracts under the heating alcohol water condition better, and based on this point, the present invention has taked the heating alcohol water extraction.For the effective ingredient of understanding at present in the Cornu Cervi Pantotrichum is lost, obtain the product of comprehensive nutrition simultaneously to greatest extent, combine traditional decocting method at last and further extract.Compare with simple low temperature pyrolyzer extract through the bright Cornu Cervi Pantotrichum extract that above-mentioned processing obtains, the content of polypeptide increases significantly.Total amino acids content is to contain 4g in the bright fine and soft extracting solution of per kilogram in the low temperature supersound extraction liquid, and through after the high-temperature process, total amino acids content is about and contains 56g in the bright fine and soft extract of per kilogram, and the polypeptides matter extraction ratio improves more than 10 times.Like this,, both guaranteed bioactive substance unlikely inactivations in leaching process such as enzyme, and can guarantee that again other extracts active ingredients were abundant simultaneously through low temperature and pyritous stage extraction.
4, this preparation method is improved the method for previously used filter membrane degerming.Though filter membrane degerming technology can keep the extract at low temperature thing not make the enzyme material inactivation again in degerming, we consider that in degerming some macromolecular material has the probability of losing.Simultaneously, the form of product is a liquid after the filter membrane degerming, and so follow-up pouring process is very high to the requirement of aseptic condition, causes controllable quality very difficult.This method adopts freeze-dry process, and after reclaiming ethanol, lyophilization becomes dry powder, can avoid contamination by micro in this process.The high temperature extraction thing obtains dry powder through after the spray drying, and this process itself also is the process of sterilization.Simplified the sterilization requirement in the production process like this, greatly.
5, in this method course of processing, adopted the broken method that combines with ultrasonic extraction of colloid mill, intracellular active substance is fully discharged, and by high efficiency extraction.Supersound extraction is compared with simple stirring and leaching, and active substance SOD, AKP and polypeptide extraction ratio exceed about 25%.
6, because final products are dry powder, original advantage is arranged than present oral liquid or other fluid product.At first be to be easy to store transportation, the product of low temperature filter membrane degerming, requires to preserve under cryogenic conditions for fear of the white enzyme or the effect of other enzyme because it exists for liquid form, bring difficulty for like this storing of product, and the product of dry powder form does not have this defective.In addition, after dry powder package becomes capsule, taking convenience, peculiar Cornu Cervi Pantotrichum abnormal smells from the patient helps the popularization of product in the no oral liquid.
Description of drawings
Fig. 1 is the process chart of this preparation method
The specific embodiment
The present invention will be further described for an embodiment below: get aquatic foods and freeze 1 kilogram in Cornu Cervi Pantotrichum and be cut into the thick fine and soft sheet of 2mm, ground 20 minutes with colloid mill, add 5 kilograms of concentration and be 50% ethanol, stir, through ultrasonic disruption instrument break process 20 minutes, then with centrifugal 30 minutes of 5000rpm supernatant and precipitation two parts.With supernatant part concentrating under reduced pressure, temperature is less than 30 ℃, be concentrated into volume and be original 1/5, freezing after, be dried to dry powder through freeze dryer; It is 50% ethanol that precipitation part is added 5 kilograms of concentration, is heated to 60 ℃, and ultrasonic disruption was handled 20 minutes, with 5000rpm centrifugal 30 minutes, and supernatant and precipitation two parts again.With this supernatant part concentrating under reduced pressure, temperature is concentrated into 1/5 of original volume less than 30 ℃ again, freezing after, be dried to dry powder through freeze dryer; And this precipitation part adds 5 kilograms of distilled water again, boiling reflux 30 minutes, with 5000rpm centrifugal 30 minutes, precipitation is discarded, and with supernatant part concentrating under reduced pressure, temperature is 60 ℃, be concentrated into 1/2 of original volume, be dried to dry powder for 60 ℃ with temperature through the drying under reduced pressure case.At last, be finished product after the power-product mix homogeneously packing that above three steps is obtained.
Claims (1)
1, the preparation method of fresh pilose antler active cell extract freeze-dried powder, it is characterized in that: get aquatic foods and freeze Cornu Cervi Pantotrichum and be cut into the thick fine and soft sheet of 1.5～2.5mm, ground 15～25 minutes with colloid mill, add 5 times of weight concentrations and be 50% ethanol, stir, through ultrasonic disruption instrument break process 15～25 minutes, got supernatant and precipitation two parts in centrifugal 30 minutes with 5000rpm then, with supernatant part concentrating under reduced pressure, temperature is less than 30 ℃, be concentrated into volume and be original 1/5, freezing after, be dried to dry powder through freeze dryer; It is 50% ethanol that precipitation part is added 5 times of weight concentrations, be heated to 60 ℃, ultrasonic disruption was handled 15～25 minutes, with 5000rpm centrifugal 30 minutes, supernatant and precipitation two parts again were again with this supernatant part concentrating under reduced pressure, temperature is less than 30 ℃, be concentrated into 1/5 of original volume, freezing after, be dried to dry powder through freeze dryer; And this precipitation part adds the distilled water of 5 times of weight again, boiling reflux 30 minutes, with 5000rpm centrifugal 30 minutes, precipitation is discarded, and with supernatant part concentrating under reduced pressure, temperature is 60 ℃, be concentrated into 1/2 of original volume, be dried to dry powder through the drying under reduced pressure case for 60 ℃ with temperature, last, be finished product after the power-product mix homogeneously packing that above three steps are obtained.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008101801389A CN101411721A (en) | 2008-12-01 | 2008-12-01 | Method for preparing fresh pilose antler active cell extract freeze-dried powder |
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| CNA2008101801389A CN101411721A (en) | 2008-12-01 | 2008-12-01 | Method for preparing fresh pilose antler active cell extract freeze-dried powder |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843639A (en) * | 2010-03-24 | 2010-09-29 | 郑彬 | Method for preparing antler freeze-dried powder by ultrasonic cracking |
| CN102228473A (en) * | 2011-06-03 | 2011-11-02 | 范圣刚 | Extraction method for active substances in cornua cervi pantotrichum |
| CN102302515A (en) * | 2010-03-19 | 2012-01-04 | 董玲 | Hairy antler freeze-dried excipient and production method thereof |
| WO2012122678A1 (en) * | 2011-03-16 | 2012-09-20 | Dong Ling | Uniform freeze-dried preparation containning all components of pilose antler and production method thereof |
| CN102860385A (en) * | 2012-08-15 | 2013-01-09 | 中华全国供销合作总社杭州茶叶研究所 | Instant mushroom tea and preparation method thereof |
| CN106478800A (en) * | 2016-11-29 | 2017-03-08 | 广西大学 | The separation and extraction technology of Cervus nippon Temminck fresh young pilose antler A44 gene active ingredient |
| CN107149139A (en) * | 2016-03-04 | 2017-09-12 | 北京圣伦食品有限公司 | A kind of preparation method for clarifying instant matsutake powder |
| CN107460089A (en) * | 2017-08-25 | 2017-12-12 | 贵州布医坊民族特色用品有限公司 | A kind of ginseng deer antler wine making apparatus |
| WO2019144485A1 (en) * | 2018-01-23 | 2019-08-01 | 广州资生生物科技有限公司 | Preparation method for picometer-scale cell extract |
| CN114934087A (en) * | 2022-05-27 | 2022-08-23 | 吉林省长白山生物科技有限公司 | Processing method and application of pilose antler blood peptide |
-
2008
- 2008-12-01 CN CNA2008101801389A patent/CN101411721A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102302515A (en) * | 2010-03-19 | 2012-01-04 | 董玲 | Hairy antler freeze-dried excipient and production method thereof |
| CN101843639A (en) * | 2010-03-24 | 2010-09-29 | 郑彬 | Method for preparing antler freeze-dried powder by ultrasonic cracking |
| CN101843639B (en) * | 2010-03-24 | 2012-04-18 | 郑彬 | Method for preparing antler freeze-dried powder by ultrasonic cracking |
| WO2012122678A1 (en) * | 2011-03-16 | 2012-09-20 | Dong Ling | Uniform freeze-dried preparation containning all components of pilose antler and production method thereof |
| CN102228473A (en) * | 2011-06-03 | 2011-11-02 | 范圣刚 | Extraction method for active substances in cornua cervi pantotrichum |
| CN102228473B (en) * | 2011-06-03 | 2012-09-19 | 范圣刚 | Extraction method for active substances in cornua cervi pantotrichum |
| CN102860385A (en) * | 2012-08-15 | 2013-01-09 | 中华全国供销合作总社杭州茶叶研究所 | Instant mushroom tea and preparation method thereof |
| CN107149139A (en) * | 2016-03-04 | 2017-09-12 | 北京圣伦食品有限公司 | A kind of preparation method for clarifying instant matsutake powder |
| CN106478800A (en) * | 2016-11-29 | 2017-03-08 | 广西大学 | The separation and extraction technology of Cervus nippon Temminck fresh young pilose antler A44 gene active ingredient |
| CN107460089A (en) * | 2017-08-25 | 2017-12-12 | 贵州布医坊民族特色用品有限公司 | A kind of ginseng deer antler wine making apparatus |
| WO2019144485A1 (en) * | 2018-01-23 | 2019-08-01 | 广州资生生物科技有限公司 | Preparation method for picometer-scale cell extract |
| CN114934087A (en) * | 2022-05-27 | 2022-08-23 | 吉林省长白山生物科技有限公司 | Processing method and application of pilose antler blood peptide |
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Open date: 20090422 |