CN101411468A - Pine needle extract of slash pine and use thereof in food fresh keeping - Google Patents
Pine needle extract of slash pine and use thereof in food fresh keeping Download PDFInfo
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- CN101411468A CN101411468A CNA2008101625315A CN200810162531A CN101411468A CN 101411468 A CN101411468 A CN 101411468A CN A2008101625315 A CNA2008101625315 A CN A2008101625315A CN 200810162531 A CN200810162531 A CN 200810162531A CN 101411468 A CN101411468 A CN 101411468A
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Abstract
The invention relates to a pine needle extract and application thereof, in particular to a slash pine water extract and application thereof in food preservation. The slash pine needle extract is light brown powder obtained by the steps of washing fresh slash pine needle by water, drying, cutting the fresh slash pine needle into short sections, adding water, extracting in boiling water bath, filtering to obtain filtrate, condensing the filtrate at reduced pressure into paste, and then drying the paste in vacuum. The invention also discloses the application of the slash pine needle extract to food sterilization and preservation. The obtained slash pine needle extract has stronger inhibiting effect on gram-positive bacteria, gram-negative bacteria and mould fungus, and has stronger oxidation resistance; the preservative has favorable stability for heat and wider pH use range; and the pine needle extract is dissolved into solution with different concentrations by water, so as to be used for preservation of different food and has good preservation effect.
Description
Technical field
The present invention relates to a kind of extract from pine needles and application thereof, relate in particular to wet-land pine tree water extract and the application in food fresh keeping thereof.
Background technology
Along with the raising of living standards of the people with to the growing interest of health, the notion of healthy food, pollution-free food is more and more accepted by masses, people more and more tend to use as far as possible in food processing and storage natural goods as anti-corrosive fresh-keeping, and the natural antisepsis fresh-keeping agent of therefore developing efficiency natural, safety non-toxic is subjected to common concern.China's pine genus plant aboundresources, kind is numerous, pine needle is one of main accessory substance of pine genus plant, be a kind of reproduction speed soon, all can gather throughout the year, widely distributed, natural accumulation is big, the natural regenerated resources of sustainable use.Pine genus plant has effects such as antibacterial, anti-oxidant, the Nanutal food fresh-keeping agent in exploitation pine needle source, and this will help exploitation, the utilization of pine needle resource.China has planted large-area wet-land pine tree from the middle and later periods nineties middle and later periods seventies 20th century to 20th century in many places, has formed a plurality of artificial swamp pine forests district.This not only extensive but also relative distributed pole of concentrating helps the processing and utilization of pine needle.
At present the research of wet-land pine tree pine needle is mainly laid particular emphasis on the research of wet-land pine tree pine needle volatile oil, wet-land pine tree pine needle essential oil extraction method is disclosed as " the GC/MS analysis of masson pine and wet-land pine tree pine needle volatile oil is produced in the Liuzhou " (mass spectrum journal the 29th is rolled up in March, 2008 the 2nd phase), the fresh pine needle sample that takes by weighing the 500.0g pulverizing places the 2000mL three-neck flask, press waterleaf and added distilled water than 2: 1, in homemade steam distillation device, distill then, distillate i.e. termination distillation until no grease, obtain having little yellow oily liquid of delicate fragrance breath.After the water-oil separating, use anhydrous sodium sulfate drying, preserve down in 0～5 ℃, to be analyzed.
Pine needle volatile oil be present in a class in the pine needle have aromatic odor, can with steam distillation come out and with the not miscible volatility oily composition of water, mainly produce by steam distillation, see with ter penoids in its composition more, be soluble in the organic solvents such as ether, chloroform, benzinum, solubility in water is very little, mostly is liquid under the normal temperature greatly.Because therefore poorly water-soluble needs to add organic solvent when in use, can't guarantee pure natural.
Summary of the invention
An object of the present invention is to provide a kind of wet-land pine tree pine needle water extract, it is a kind of natural product, has the advantages that water-soluble can be good.Another object of the present invention provides a kind of purposes of wet-land pine tree extract from pine needles.The 3rd purpose of the present invention provides a kind of food antimicrobial antistaling agent, and it has good stable to heat, has the pH scope of application of broad, and fresh-keeping effect is remarkable.
In order to realize first above-mentioned purpose, the present invention has adopted following technical scheme:
The wet-land pine tree extract from pine needles, this wet-land pine tree extract from pine needles washes for adopting fresh wet-land pine tree pine needle, drains, and is cut into short broken section, adds water, and boiling water bath extracts, and crosses leaching filtrate, is evaporated to paste, then the light brown powder that obtains of vacuum drying.
As preferably, above-mentioned amount of water is 10～14 times of fresh wet-land pine tree pine needle weight, and boiling water bath extraction time is 1.5～3.0h.
As preferably, above-mentioned decompression concentrates and vacuum drying temperature is 50～60 ℃.
Wet-land pine tree extract from pine needles main component of the present invention is a flavone compound, and Main Ingredients and Appearance is different from pine needle volatile oil, and wet-land pine tree extract from pine needles of the present invention is soluble in water, can be made into solid and preserves.The yield of wet-land pine tree extract from pine needles is 10-14%.
In order to realize second above-mentioned purpose, wet-land pine tree extract from pine needles of the present invention is used for the food anti-bacterial refreshing.Using method is that the wet-land pine tree extract from pine needles is configured to the aqueous solution, and the wet-land pine tree extract from pine needles aqueous solution joins in the food.
In order to realize the 3rd above-mentioned purpose, the present invention has adopted following technical scheme:
A kind of food antimicrobial antistaling agent, this antistaling agent comprise above-mentioned wet-land pine tree extract from pine needles.Certainly can add other auxiliary materials or not add other auxiliary materials as a kind of food antimicrobial antistaling agent.
The wet-land pine tree extract from pine needles that the present invention obtains has stronger inhibitory action to gram-positive bacteria, Gram-negative bacteria, mould, and has a stronger anti-oxidation function, this antistaling agent has good stable to heat, the pH scope of application with broad, water is dissolved into variable concentrations solution with extract from pine needles, it is fresh-keeping to be used for different food products, and fresh-keeping effect is obvious.
Description of drawings
Fig. 1 is that variable concentrations wet-land pine tree pine needle water extract is removed the superoxide radical capability chart.
The specific embodiment
Embodiment 1
The preparation method of wet-land pine tree extract from pine needles:
1. gather fresh wet-land pine tree pine needle, the running water flushing drains.
2. the above-mentioned pine needle of 100g is cut into broken section of about 1cm length, adds the 1000ml running water, boiling water bath extracts 1.5h.
3. cross leaching filtrate, decompression concentrates (50 ℃) to paste.
4. vacuum drying (50 ℃) gets light brown powder 10.9g.
The preparation method of wet-land pine tree extract from pine needles:
1. gather fresh wet-land pine tree pine needle, the running water flushing drains.
2. the above-mentioned pine needle of 80g is cut into broken section of about 1cm length, adds the 1120ml running water, boiling water bath extracts 2.5h.
3. cross leaching filtrate, decompression concentrates (60 ℃) to paste.
4. vacuum drying (55 ℃) gets light brown powder 9.8g.
Embodiment 3
The preparation method of wet-land pine tree extract from pine needles:
1. gather fresh wet-land pine tree pine needle, the running water flushing drains.
2. the above-mentioned pine needle of 80g is cut into broken section of about 1cm length, adds the 480ml running water, boiling water bath extracts 1.5h, filters, and filter residue adds the 480ml running water again, and boiling water bath extracts 1.5h, filters merging filtrate.
3. filtrate decompression concentrates (55 ℃) to paste.
4. vacuum drying (50 ℃) gets light brown powder 10.9g.
The wet-land pine tree pine needle water extract that the inventive method obtains is light brown powder, good water solubility.
Test example 1
The bacteriostatic experiment of the wet-land pine tree pine needle water extract that the inventive method obtains
Escherichia coli are common fecal pollution indicator bacterias, and proteus can cause food poisoning, and the both belongs to gramnegative bacterium, are common food spoilage bacterium.As can be seen from Table 1, the wet-land pine tree extract from pine needles all has certain inhibitory action to the growth of these two kinds of bacteriums, and along with the reduction for the examination substrate concentration, inhibitory action weakens.Being better than colibacillary effect for the inhibitory action of examination thing to proteus growth, is 0.04g/ml to colibacillary minimum inhibitory concentration, and is 0.02g/ml to the minimum inhibitory concentration of proteus.
The wet-land pine tree extract from pine needles of table 1 variable concentrations is to the inhibitory action [antibacterial circle diameter (cm)] of Escherichia coli and proteus growth
Hay bacillus, bacillus megaterium etc. supply the examination bacterium all to belong to gram-positive bacterium for five kinds, also be common food spoilage bacterium, as can be seen from Table 2, the wet-land pine tree extract from pine needles all has certain inhibitory action to the growth of these five kinds of bacteriums, high more for the examination substrate concentration, bacteriostasis is strong more; From table, it can also be seen that, the strongest to Bacillus cercus and aureus with inhibition under same concentrations for examination thing inhibiting strong and weak different to different bacterium, and the staphylococcic inhibitory action of dialogue relatively a little less than; Also be not quite similar for the minimum inhibitory concentration of examination thing simultaneously to these five kinds of bacteriums, wherein the staphylococcic minimum inhibitory concentration of dialogue is 0.04g/ml, minimum inhibitory concentration to hay bacillus, bacillus megaterium, Bacillus cercus is 0.03g/ml, and the minimum inhibitory concentration of staphylococcus aureus is reached 0.02g/ml.
The wet-land pine tree extract from pine needles of table 2 variable concentrations is to the inhibitory action [antibacterial circle diameter (cm)] of hay bacillus etc.
Aspergillus niger is commonly in the food to cause one of mould, as can be seen from Table 3, and under for examination concentration, water extract all has significant inhibitory effect to the growth of aspergillus niger, compared with the control, shows as the bacterium colony loop diameter and reduces, mycelial density weakens, and sporulation quantity obviously reduces, and the mycelial growth amount descends.Compare for the examination thing testing the influence of used several bacterial growths, stronger to the inhibitory action of aspergillus niger growth for the examination thing, the growth to aspergillus niger when being low to moderate 0.001g/ml for the examination substrate concentration also has utmost point significant inhibitory effect.
The wet-land pine tree extract from pine needles of table 3 variable concentrations is to the inhibitory action of aspergillus growth
The growth of bacterium, mould itself can be subjected to the influence of pH value, and as can be seen from Table 4, pH=4,5 o'clock hay bacilluses do not grow, and pH=6 is a little less than growing, so can't measure the bacteriostasis of pine needle crude extract during pH4-6.The wet-land pine tree water extract all has very strong inhibitory action in the pH7-9 scope to hay bacillus.As shown in Table 5, aspergillus is not grown when pH4.Under the pH5-9 condition, the wet-land pine tree water extract has very strong inhibitory action to the growth of aspergillus.
Table 4 substrate pH is to the influence (representing the cm of unit with antibacterial circle diameter) of the anti-hay bacillus activity of pine needle water extract
Annotate: "-" expression hay bacillus does not grow; "+" expression hay bacillus grows less but can't measure inhibition zone.
Table 5 substrate pH is to the influence (representing the cm of unit with the bacterium colony loop diameter) of the anti-aspergillus activity of pine needle water extract
Annotate: "-" expression aspergillus is not grown.
As can be seen from Table 6, after 121 ℃ of high-temperature process, the bacteriostatic activity of sample does not become compared with the control substantially, the almost not influence of handling between high temperature, short time the wet-land pine tree extract from pine needles of bacteriostasis is described, wet-land pine tree pine needle water extract antibacterial substance has good heat endurance.
Table 6. temperature suppresses the effect of hay bacillus bacterium to wet-land pine tree pine needle water extract
Test example 2
The anti-oxidant experiment of the wet-land pine tree pine needle water extract that the inventive method obtains
With ascorbic acid (Vc) (concentration 0.1%) is reference, is contrast with the lard (CK) that does not add any sample, regularly measures the peroxide value (POV value) of grease.With the content of the corresponding peroxide of POV value, the oxidized degree of high more expression sample is big more.POV value result of variations in time sees Table 7.
Table 7. wet-land pine tree extract from pine needles (0.1g/ml) and 0.1%Vc are to the oxidation resistance (POV) of lard
Annotate: the same time period, compare with CK (blank), a represents p＜0.05, b represents p＜0.01
Same processing is compared with previous time period, and A represents p＜0.05, and B represents p＜0.01
As shown in Figure 1, in illumination riboflavin system, wet-land pine tree pine needle water extract is to superoxide radical (O
2-) clearance rate experiment show that finite concentration wet-land pine tree pine needle water extract has certain removing ability to superoxide radical, the clearance rate that 0.5-10mg/ml concentration wet-land pine tree water extract is removed superoxide radical is up to about 80.0%.
Test example 3
The wet-land pine tree pine needle water extract that the inventive method obtains is to the preserve test of different food products
Be configured to the solution of 25mg/ml with wet-land pine tree pine needle water extract.With cucumber, watermelon, pineapple is pressed into fruit juice with juice extractor, the fruit juice of measuring 20mL is put into container, adds the above-mentioned wet-land pine tree pine needle water extract solution of different amounts then, is contrast not loosen the pin extract, room temperature was placed after 24 hours, observed the color of fruit juice, situations such as smell situation of change and long bacterium.
Get the dried bean curd of several parts of equivalent, the clear water of respectively putting into 20mL boils, and each adds the above-mentioned wet-land pine tree pine needle water extract solution of different amounts in the process of boiling.Not add the dried bean curd that boils in the pine needle extract clear water is contrast.Room temperature is placed the color of observing dried bean curd after 24 hours, smell, quality situation of change and rotten degree etc.
Food antiseptic effect behind the table 8. wet-land pine tree pine needle water extract processing 24h
Experimental result shows, control series was obviously rotten after 24h was carried out in experiment, and add the wet-land pine tree water extract certain antiseptic effect was arranged, its antiseptic effect along with adding wet-land pine tree water extract amount increase and obviously improve.
Compare the conduct of wet-land pine tree pine needle water extract with traditional Sodium Benzoate, potassium sorbate antistaling and antiseptic agent Anticorrosion and the antiseptic of food has the advantages such as green, health care. Day by day obtain paying attention in food security Modern society in, in the cry of a slice back to nature, exploitation pine needle water extract is done natural anticorrosion fresh-keeping Agent is bound to be subjected to the favor of international market, more is subjected to the welcome of domestic market.
Claims (6)
1. wet-land pine tree extract from pine needles is characterized in that: this wet-land pine tree extract from pine needles washes for adopting fresh wet-land pine tree pine needle, drains, be cut into short broken section, add water, boiling water bath extracts, cross leaching filtrate, be evaporated to paste, then the light brown powder that obtains of vacuum drying.
2. require 1 described a kind of wet-land pine tree extract from pine needles according to claim, it is characterized in that: amount of water is 10～14 times of fresh wet-land pine tree pine needle weight, and boiling water bath extraction time is 1.5～3.0h.
3. require 1 or 2 described a kind of wet-land pine tree extract from pine needles according to claim, it is characterized in that: decompression concentrates and vacuum drying temperature is 50～60 ℃.
4. wet-land pine tree extract from pine needles according to claim 1 is used for the food anti-bacterial refreshing.
5. wet-land pine tree extract from pine needles according to claim 4 is used for the food anti-bacterial refreshing, it is characterized in that: the wet-land pine tree extract from pine needles is configured to the aqueous solution, and the wet-land pine tree extract from pine needles aqueous solution joins in the food.
6. food antimicrobial antistaling agent, it is characterized in that: this antistaling agent comprises the described wet-land pine tree extract from pine needles of claim 1.
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| CN2008101625315A CN101411468B (en) | 2008-12-01 | 2008-12-01 | Pine needle extract of slash pine and use thereof in food fresh keeping |
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| CN101411468B CN101411468B (en) | 2012-07-04 |
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| CN102613281A (en) * | 2012-04-19 | 2012-08-01 | 韶关学院 | Preparation method for south China pine needle extractives and application of south China pine needle extractive in freshness of eggs |
| CN102763806A (en) * | 2012-07-16 | 2012-11-07 | 陆思烨 | Blueberry health-promoting steamed bun |
| CN102763806B (en) * | 2012-07-16 | 2013-10-09 | 陆思烨 | Blueberry health-promoting steamed bun |
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| CN104365680B (en) * | 2014-09-28 | 2017-02-22 | 江苏农林职业技术学院 | Slash pine needle extract and use thereof |
| CN104365680A (en) * | 2014-09-28 | 2015-02-25 | 江苏农林职业技术学院 | Slash pine needle extract and use thereof |
| CN104621243A (en) * | 2015-02-05 | 2015-05-20 | 浙江师范大学 | Plant-sourced anti-browning agent for fruits and vegetables, as well as preparation method and application thereof |
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| CN108261112A (en) * | 2016-08-21 | 2018-07-10 | 王琪 | A kind of walnut opener |
| CN108294656A (en) * | 2016-08-21 | 2018-07-20 | 王琪 | A kind of nut fruit openers |
| CN108451406A (en) * | 2016-08-21 | 2018-08-28 | 王琪 | Nut fruit openers |
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