CN101405038A - Porous bioabsorbable material and method of producing the same - Google Patents

Abstract

Provided is a porous bioabsorbable material in the shape of a film which is a dense construct having a small average pore size and an even pore size and showing a large maximum stress, in particular, a porous bioabsorbable material which is highly useful as a material for preventing adhesion. A porous bioabsorbable material characterized by comprising a bioabsorbable polymer which is constructed by gelling a bioabsorbable polymer by using a good solvent compatible with the bioabsorbable polymer and a poor solvent and freeze drying the thus gelled bioabsorbable polymer to give a porous structure; and a method of producing a porous bioabsorbable material characterized by comprising gelling a bioabsorbable polymer by using a solvent mixture composed of a good solvent compatible with the bioabsorbable polymer and a poor solvent and freeze drying the gelled product thus gelled to give a porous structure.

Description

Porous bioabsorbable material and manufacture method thereof

Technical field

The present invention relates to porous body, particularly relating to is being porous bioabsorbable material and manufacture method thereof useful in the medical field at center with organizational project or regenerative medicine engineering.

Background technology

The employed bioabsorbable material of the intravital material of implantable bioartificial mainly is used to regenerative medicine with timbering material or adherence preventing material.The former is the regenerative medicine timbering material, in order to make cell portion's propagation within it, preferably uses porous body.So long as porous body, repopulating cell makes its propagation in its hole, it is transplanted in the organism again, causes tissue regeneration in vivo, simultaneously, is decomposed at leisure in vivo, absorbs as the bioabsorbable material of support.Thereby, can will be used for the support of cell proliferation directly in proliferative cell is transplanted to organism.And, using the bioresorbable polymer, invading in the porous body in order to make cell as the occasion of this porous regenerative medicine with timbering material, the pore-size of wishing porous body is through bigger.

As the manufacture method of above-mentioned porous regenerative medicine with the employed porous body of timbering material, for example, people have known the freeze-drying method described in following patent documentation (1)～(4).In addition, the inventor has also proposed a kind of technical scheme, handle by mixed solution described below being carried out lyophilization, can solve above-mentioned prior art problems from the small-bore to wide-aperture wide region inner control aperture, and make manufacturing engineering simple bioresorbable polyalcohol stephanoporateization and make bioabsorbable material (patent documentation 5).

Patent documentation 1: the spy opens flat 10-234844 communique

Patent documentation 2: the spy opens the 2001-49018 communique

Patent documentation 3: special table 2002-541925 communique

Patent documentation 4: the spy opens flat 02-265935 communique

Patent documentation 5: special Willing 2005-80059

Summary of the invention

The task that the present invention will solve

The employed porous bioabsorbable material of porous bioabsorbable material, particularly adherence preventing material has and is used for supplying with to the bio-tissue that contacts with this material the loose structure of nutritional labeling etc.In order not allow cell invade in its hole, to produce the tissue adhesion, its pore-size must be less but simultaneously.Have, usually, adherence preventing material uses with film shape again, even thereby the bioabsorbable material of porous membrane shape, also wish to have big stress.

But employing is the film-forming method of porous bioabsorbable material commonly used at present, can not make the porous membrane that satisfies above-mentioned requirements.For example, the thin film of the porous bioabsorbable material that 300 μ m are following, its intensity is low excessively, can not sew up, and breaks easily, perforate, is difficult for peeling off etc. from model, has a lot of problems.In order to address the above problem, people consider to strengthen the thickness of adherence preventing material.But, so, adherence preventing material is inserted, is configured to the position that needs treatment become difficult.Therefore, be that porous membrane has big intensity, particularly has a porous bioabsorbable material as the needed intensity of adherence preventing material although be difficult to obtain.

The invention provides a kind of porous bioabsorbable material, particularly can be as the porous bioabsorbable material and the manufacture method thereof of adherence preventing material, described porous bioabsorbable material can prevent the intrusion of cell, has simultaneously the less hole that is fit to see through materials such as nutrient equably, and, though be that porous body has big maximum stress.

Be used to solve the measure of above-mentioned task

The inventor is by providing the manufacture method of porous bioabsorbable material described below and this porous bioabsorbable material, thereby can solve above-mentioned technical assignment, the feature of described porous bioabsorbable material is, the maximum stress of this bioabsorbable material is 3～23MPa, the porosity is 0.1～82%, and aperture (on average) (hereinafter being also referred to as average pore size) is 9～34 μ m.

Porous bioabsorbable material of the present invention can be made by following operation: use bioresorbable polymer and the good solvent that has compatibility each other of this bioresorbable polymer and the operation that Weak solvent prepares the gelation thing; Above-mentioned gelation thing is carried out the operation of freezing processing; And the operation of the above-mentioned freezing processing thing of drying under reduced pressure.Above said good solvent, be meant the solvent bigger to the dissolubility of bioresorbable polymer, in addition, said Weak solvent is meant the solvent smaller to the dissolubility of bioresorbable polymer.

The operation for preparing above-mentioned gelation thing is undertaken by the following stated, promptly, the use level of the Weak solvent of the mixture that constitutes by bioabsorbable material, above-mentioned good solvent and Weak solvent at least by control, said mixture is separated into is solvent phase and gel phase, the use level of the above-mentioned needed Weak solvent that is separated for example constitutes the composition of monomer component of above-mentioned bioabsorbable material or combination and ratio, the environment temperature etc. of ratio of components, molecular weight, above-mentioned bioabsorbable material and good solvent and Weak solvent and changes according to various essential conditions.

Below, explain the present invention according to embodiment.

1. bioresorbable polymer

As the bioresorbable polymer among the present invention, for example can enumerate the copolymer of lactide and caprolactone.This copolymer can be any in atactic polymer, the block polymer, and its molecular weight (weight average molecular weight) has no particular limits, and for example is 5000～2000000, preferably 10000～1500000, preferably 100000～1000000.In addition, the mol ratio of lactide and caprolactone for example is 90: 10～10: 90 a scope, preferably 85: 15～20: 80 scope, preferably 80: 20～40: 60 scope.

The polymerization of the copolymer of above-mentioned lactide and caprolactone has no particular limits, and can use known method in the past.For example,, can make lactide and caprolactone carry out copolymerization by ring-opening polymerisation as initiation material, also can be by synthesis of lactide from lactic acid (cyclic dimer of lactic acid), make itself and caprolactone copolymerization.

Above-mentioned lactide can use L-lactide, D-lactide and their mixture (D, L lactide), in addition, as lactic acid, can use L-lactic acid, D-lactic acid and their mixture (D, L-lactic acid).Use the occasion of lactic acid like this, monomeric lactic acid is converted into lactide, the lactide that converted and the preferably above-mentioned scope of the mol ratio of caprolactone of dimer as initiation material.In addition,, for example can enumerate 6-caprolactone, gamma-butyrolacton, δ-Wu Neizhi, wherein, especially preferentially select 6-caprolactone as lactone.

In the above description, bioresorbable polymer as the object of porous of the present invention, copolymer with lactide and caprolactone is that example is illustrated particularly, but, even other bioresorbable polymer, as long as utilize the above-mentioned solvent that good solvent and Weak solvent by this polymer constitute can gelation, be also included within the multiporous biological absorbable polymer of the present invention.As such bioresorbable polymer, except lactide and caprolactone, the copolymer composition that can also contain the bioresorbable polymer that constitutes other is as constituent, and such copolymer composition can be enumerated by glycolic, propylene carbonate, beta-hydroxy-butanoic acid, protein, the deutero-copolymer composition of saccharide.

2. gelation thing

The gelation thing for preparing the above-mentioned bioresorbable polymer that uses among the present invention by the following stated: in the mixture of miscible each other Weak solvent with above-mentioned bioresorbable polymer, this bioresorbable polymer and good solvent, as above-mentioned Weak solvent, cooperation is with the necessary amount of above-mentioned bioresorbable polymer gelization, above-mentioned bioresorbable polymer formation gelation state is separated, and the thing that will be separated separates.In addition, the amount of the above-mentioned bioresorbable polymer in the said mixture has no particular limits, normally 0.1～24 quality %, preferably 2～8 quality %, preferably 3～5 quality %.

3. solvent

The miscible each other above-mentioned good solvent and the kind of Weak solvent, for example can be according to the kind decision of used bioresorbable polymer, the necessary Weak solvent of copolymer as above-mentioned lactide of preparation and caprolactone, can make water, ethanol, the tert-butyl alcohol (tBuOH) etc., in addition, as good solvent, can use with above-mentioned Weak solvent and demonstrate 1 of the compatibility, organic solvents such as 4-diox, dimethyl carbonate etc., especially preferentially selecting Weak solvent is that water, good solvent are 1, the combination of 4-diox.

Has above-mentioned bioresorbable polymer, in the mixture of Weak solvent and good solvent, use level with the necessary Weak solvent of above-mentioned bioresorbable polymer gelization, can be according to the bioresorbable polymer that constitutes this mixture, the kinds of Weak solvent or good solvent etc. are suitably determined, if the use level of this Weak solvent is less than making said mixture form the necessary amount of gelation state, just can not form gelation state, otherwise, form the necessary amount of gelation state if surpass, the bioresorbable polymer will be superfluous and coagulation takes place, a part becomes filminess, can not form porous fully even carry out lyophilization.Therefore, form the amount of the necessary Weak solvent of gelation state, suitably determine according to employed every-kind of mixture with bioresorbable polymer, Weak solvent and good solvent in order to make said mixture.In addition, by in the scope that can prepare above-mentioned gelation thing, changing the use level of Weak solvent, can control the porosity (as shown in table 3) of multiporous biological absorbable polymer, the maximum stress (as table 4 and shown in Figure 1) of multiporous biological absorbable polymer or the average pore size (as table 2, table 6～8, shown in Figure 3) of multiporous biological absorbable polymer.

Freezing process

Above-mentioned gelation thing freezing can use known refrigerating process and refrigerating plant.In addition, the cryogenic temperature of gelation thing so long as the following temperature of the eutectic point that this gelation thing is freezed fully get final product, there is not any restriction, occasion at the above-mentioned bioresorbable polymer gelation thing that is lactide and caprolactone copolymer, preferably-3 ℃, the scope below preferably-10 ℃.In addition, when changing the rate of cooling of gelation thing, the hole dimension of resulting porous bioabsorbable material can change, and therefore, by selecting the rate of cooling of gelation thing, can control the hole dimension of porous bioabsorbable material.

Description of drawings

Fig. 1 is the figure of maximum stress of the porous bioabsorbable material of expression embodiment 1 and comparative example.

Fig. 2 is the figure of the maximum stress assay method of expression porous bioabsorbable material.

Fig. 3 is the figure of the average pore size of expression porous bioabsorbable material.

Fig. 4 is 300 times the section photo with ultramicroscope (SEM) shooting of porous body E.

Fig. 5 is 1000 times the surface picture with ultramicroscope (SEM) shooting of porous body E.

Fig. 6 is that the moisture adding rate is 100 times the section photo of taking with ultramicroscope (SEM) of 0% porous body A.

Fig. 7 is the figure of the glucose permeability testing machine of use among the expression embodiment 3.

Fig. 8 is the figure of the glucose permeability result of the test of expression embodiment 3.

Symbol description

1 silicone plug

2 sample taps

Room 3 (glucose one side)

4 silicone coated

The sample film that the 5 porous body thin film of being made by sample D, E and A constitute

Room 6 (RO water one side)

7 agitators

The specific embodiment

Change the moisture content in the mixed solution, make the bioresorbable polyalcohol stephanoporateization, make porous bioabsorbable material.

Embodiment 1

Make porous lactide-caprolactone copolymer by chilling, it with the ratio of components (mol ratio) of L-lactide and 6-caprolactone lactide-caprolactone copolymer (below be also referred to as the LA/CL copolymer), 1 of 75: 25,4-diox and water mix, the 17 kinds of mixed liquor 12g of the A～R shown in the following table 1 of preparation.In these samples, gelation has taken place in sample D～R, and Sample A～C is a solution state.

The composition of Sample A～R is shown in the following table 1.

Table 1

Sample	Add moisture rate (%)	P(LA/CL)	Diox	Water
					A	0	4.00	96.00	0
B	5	3.80	91.20	5
					C	10	3.60	86.40	10
D	12	3.52	84.48	12
					E	13	3.48	83.52	13
F	14	3.44	82.56	14
					G	15	3.40	81.60	15
H	16	3.36	80.64	16
					I	17	3.32	79.68	17
J	18	3.28	78.72	18
					K	19	3.24	77.76	19
L	20	3.20	76.80	20
					M	22	3.12	74.88	22
N	25	3.00	72.00	25
					O	30	2.80	67.20	30
P	35	2.60	62.40	35
					R	40	2.40	57.60	40

Comprise the record of top table 1, in the present invention, " interpolation moisture rate " is meant weight %.

Said sample D～R is separated, with the gelation thing separation of above-mentioned L-lactide-ε caprolactone copolymer.Should infeed respectively in the rustless steel culture dish by isolating gelation thing, above-mentioned gelation thing is configured as 0.5mm thickness.In addition, the Sample A with solution state also infeeds in the rustless steel culture dish.The culture dish of putting into above-mentioned gelation thing or solution is placed on being cooled on-50 ℃ the cooling shelf of freezer dryer, for example TF5-85ATANCS (trade name, precious make manufacturing), with carrying out quick freezing in about 1 hour.Then, under reduced pressure, make porous body with making the temperature in the freezer dryer rise to 25 ℃ from-50 ℃ in 12 hours.The average pore size of these porous bodies (μ m) is shown among following table 2 and Fig. 3, and its porosity is shown in the following table 3 in addition.

The average pore size of the A～R of porous body shown in the table 2 below (μ m).In the following Table 2, adding moisture rate is below 10%, and average pore size is big, and this average pore size along with adding the moisture rate difference big variation takes place.In addition, as can be seen, add moisture rate 10% when following, the standard deviation of average pore size is big, and the aperture of resulting porous body has deviation to fluctuate.On the other hand, as can be seen, add moisture rate 12% when above, average pore size is little, and the standard deviation of average pore size is very little.Hence one can see that, according to the present invention, can obtain that the aperture is less, this aperture ratio is than the porous body of homogeneous.

Table 2

Sample	Add moisture rate (%)	Average pore size (μ m)	Standard deviation
				A
	0	38	6.1
				B	5	27	5.4
C	10	15	0.8
				D	12	9	0.0
E	13	12	1.4
				F	14	11	0.6
G	15	12	1.5
				H	16	13	1.3
I	17	11
				J	18	15
K	19	15
				L	20	20
M	22	13
				N	25	20
O	30	19
				P	35	21
R	40	17

300 times the section photo of above-mentioned porous body E shown in Figure 4 with ultramicroscope (SEM) shooting.In addition, the ultramicroscope (SEM) of 1000 times of the usefulness of this porous body shown in Figure 5 surface picture of taking.By these figure as can be seen, by the porous body that the gelation thing of present embodiment obtains, its aperture roughly is a homogeneous.

In contrast, LA/CL copolymer, 1 with the solution state of unmixed water, the blending ratio of 4-diox and water is the mixture (Sample A) of 4: 96: 0 (weight %), carry out the quick freezing drying similarly to Example 1, make porous body, take the section photo of this porous body with 100 times ultramicroscope (SEM), be shown among Fig. 6.As shown in Figure 6, the hole of porous body has different orientation (for example orientation being arranged laterally or vertically) in the part, and the aperture is also from significantly greatly to significantly little.

The porosity of porous body A～R (%) is shown in the following table 3.

Table 3

Sample	Add moisture rate (%)	The porosity (%)
			A	0	92
B	5	91
			C	10	92
D	12	82
			E	13	74
F	14	69
			G	15	67
H	16	61
			I	17	54
J	18	43
			K	19	45
L	20	37
			M	22	20
N	25	26
			O	30	18
P	35	12
			R	40	0.1

The maximum stress of porous body A～R (MPa) is shown in the following table 4.

Table 4

Sample	Add moisture rate (%)	Average maximum stress (MPa)
			A	0	0.4
B	5	0.5
			C	10	0.6
D	12	3.4
			E	13	4.7
F	14	5.2
			G	15	7.7
H	16	8.5
			I	17	13
J	18	12
			K	19	15
L	20	11
			M	22	21
N	25	12
			O	30	15
P	35	22
			R	40	23

Embodiment 2

According to the manufacture method of the foregoing description 1, the sample S of the solution state shown in preparation said sample A, C～H, J, L and the following table 5.Same with the foregoing description 1, these samples are made the shaping thing, adopt-3 ℃/hour rate of cooling, operate equally with the manufacture method of the porous body of the foregoing description 1, these shaping things are made porous body, in addition, operate equally with the foregoing description 1, said sample E, F, G are made the shaping thing, adopt the rate of cooling of-5 ℃/hour and-10 ℃/hour, similarly operate with the manufacture method of the foregoing description 1 porous body, these shaping things are made porous body, the average pore size of the porous body of making (μ m) is shown in table 7～8.

The composition of sample S is shown in the following table 5.

Table 5

Sample	Add moisture rate (%)	P(LA/CL)	Diox	Water
					S	6	3.76	90.24	6

The average pore size of the porous body made from-3 ℃/hour rate of cooling shown in the table 6 below.

Table 6

Sample	Add moisture rate (%)	Average maximum stress (MPa)
			A	0	100
S	6	53
			C	10	72
D	12	34
			E	13	27
F	14	26
			G	15	23
H	16	22
			J	18	23
L	20	25

In last table, add moisture rate and be 10% when following, average pore size is big, and this average pore size along with the interpolation moisture rate big variation takes place.

Be shown in the following Table 7 the average pore size of the porous body made from-5 ℃/hour rate of cooling.

Table 7

Sample	Add moisture rate (%)	Average pore size (μ m)
			E	13	23
F	14	21
			G	15	21

Be shown in the following Table 8 the average pore size of the porous body made from-10 ℃/hour rate of cooling.

Table 8

Sample	Add moisture rate (%)	Average pore size (μ m)
			E	13	20
F	14	18
			G	15	17

By the result of the foregoing description 1 and 2 as can be known, during with the gelation thing lyophilization of lactide-caprolactone copolymer, compare with embodiment 1 such quick refrigerative situation, during as embodiment 2 slow coolings, average pore size has some increases, but average pore size is all little when similarly carrying out slow cooling during with quick cooling, and the size of average pore size also reaches unanimity.Relative therewith, when by slow cooling the solution of lactide-caprolactone copolymer being carried out lyophilization, as by what top table 6, Fig. 3 and Fig. 6 saw, average pore size increases, and average pore size is also along with big variation takes place the use level of Weak solvent water.

The average pore size of the porous body of making in the foregoing description 1 and 2 is measured by the following stated.The discoideus porous body thin film that obtains is cut off, make it to expose section.With the section of the porous body sample of the above-mentioned cut-out of electron microscope observation, set enlargement ratio, make per 1 visual field can confirm about about 30 to 100 aperture, take the SEM photo.Select the hole in the aperture that 10 aperture ratio are big, the frequency of occurrences is high in the SEM photo that obtains, (NIH image) analyzes, calculates with image analysis software.

The maximum stress of porous body

Measure the maximum stress of the porous body of making in the foregoing description 1 by the following stated.Use cupping machine (trade name: ォ-ト グ ラ Off, Shimadzu Seisakusho Ltd. makes), the rectangular sample that cuts 1 * 5cm from above-mentioned discoideus porous body sample is tested as shown in Figure 2, chuck spacing during test is 20mm, draw speed is 50mm/min, measures the maximum stress of this sample by stretching.Measurement result is shown among Fig. 1.In addition, as a comparative example, (ジ ェ Application ザ ィ system ジ ャ パ Application Co., Ltd. makes, trade name: Sai Pula film (Seprafilm: bioabsorbable membrane to measure commercially available synthetic absorbability adherence preventing material, registered trade mark)) maximum stress, measurement result is shown among Fig. 1.By the result of Fig. 1 as can be known, by the gelation thing of the lactide-caprolactone copolymer (porous body of sample D～R) make, compare with the porous body of making by the solution (Sample A, B, C) of lactide-caprolactone copolymer, maximum stress is big, has and the silicone sheets same degree of non-porous body or the big maximum stress more than it.In addition, porous body of the present invention has the bigger maximum stress of maximum stress than the commercially available synthetic absorbability adherence preventing material that is used as comparative example.

The porosity of porous body

The porosity of the porous body of making in the foregoing description 1 is measured by the following stated.Discoideus porous body is cut into the square of 1.5 * 1.5cm, measure the weight of test piece.Then, measure the thickness of test piece with digital microscope (キ-ェ Application ス company makes).The density (ρ) of obtaining the porous body sample by the weight and the thickness of the sample that obtains.Use the same method again and obtain the density (ρ that uses the thin film made from the lactide-caprolactone copolymer of this porous body sample same composition ₀), be calculated as follows the porosity of giving vent to anger (%) (p).

p＝1-ρ/ρ ₀

By the result of the test of the porosity shown in the top table 3 as can be known, the porous body of making by the gelation thing of lactide-caprolactone copolymer, when the use level as the water of Weak solvent increased, the porosity reduced significantly.

From the result of the test of the foregoing description 1 and 2 as can be known, the porous body of making by the gelation thing of lactide-caprolactone copolymer, compare with the porous body of being made by the solution of identical lactide-caprolactone copolymer: (1) maximum stress is very big, (2) porosity is significantly little, be fine and close structure, the cooling means of (3) no matter lyophilization operation is chilling or slow cooling, and the variation of average pore size is all little, and the size in hole is neat, and the little porous body of average pore size is obtained by Weak solvent.Have again, the use level of the Weak solvent water by changing the preparing gel operation, the cooling means of no matter lyophilization operation is chilling or slow cooling, can both keep above-mentioned characteristic, average pore size itself also can change.Have again, from the result of the test of the foregoing description 1 as can be known, by the porous body that the gelation thing of lactide-caprolactone copolymer of sample D～R is made, the maximum stress of its bioabsorbable material is 3.4～23.1 (MPa), the porosity (%) is 0.1～82%, and average pore size is 9～21 (μ m).

Embodiment 3

The sample E (adding moisture rate 14%, thickness 150 μ m) that obtains for the manufacture method that adopts the foregoing description 1, the thin film of G (adding moisture rate 15%, thickness 170 μ m), use the testing machine that sees through shown in Figure 7, its glucose permeability is tested by the following stated.In addition, as a comparative example, carried out same research for sample C (adding moisture rate 10%, thickness 100 μ m).

Making evaluation sample 5 and silicone coated 4 is between 2 chambers (3,6) of diameter 14mm * length 80mm, their liquid thickly to be connected (screw clamp) between volume.Then, the glucose solution 10mL of filling 250mg/dL concentration in chamber 3, filling RO water 10mL in chamber 6.Then, with agitator 7 reposefully in the teeter chamber 3 and 6, every the stipulated time from the sample tap 20 μ L that materials, with its sample as test.Use glucose quantitation kit (グ Le コ-ス CII テ ス ト ヮ コ-(Wako Pure Chemical Industries, Ltd. makes, trade name)), utilize absorbance to obtain concentration of glucose in chamber 3 and 6.It the results are shown among Fig. 8.

By the result of Fig. 8 as can be seen, compare sample F (gel: add moisture rate 14%) and G (gel: interpolation moisture rate 15%) have low glucose permeability with Sample A (solution: add moisture rate 10%).In addition, sample F and sample G are compared the permeability difference of glucose.The bio-absorbable porous body that the gel that is obtained by the present invention is made has the glucose permeability, simultaneously, adds moisture rate and can control glucose and see through ability by changing in its manufacture process.

Embodiment 4

The manufacturing of adherence preventing material

Adopt the manufacture method of the foregoing description 1, in above-mentioned rustless steel culture dish, form the thin film of porous lactide-caprolactone copolymer of thickness 50～600 μ m, preferred 50～300 μ m, from above-mentioned rustless steel culture dish, take out this thin film by the gelation thing of lactide-caprolactone copolymer of sample D～R.At this moment, the maximum stress of the porous lactide-caprolactone copolymer of formation thin film is big, thereby produces be full of cracks on this thin film.In addition, as mentioned above, the hole of the thin film of porous lactide-caprolactone copolymer (hole) is less, has the average-size that reaches unanimity, and is the compact texture body with big maximum stress.Therefore, as mentioned above, although porous bioabsorbable material of the present invention is the porous membrane shape, but have big stress, and its hole (hole) is less, helps preventing the cell intrusion, and the aperture of hole (hole) also is uniform, have the glucose permeability, thereby help through materials such as nutrients, so as adherence preventing material, for example as preventing that the key adhering material is exceedingly useful.

Application possibility on the industry

Adopt the manufacture method of porous bioabsorbable material of the present invention, as shown in Figure 4, no matter the cooling means of freeze drying operation is chilling or slow cooling (3 ,-5 ,-10 ℃/hour), and the aperture all is consistent, and this consistent aperture is less. Have again, by the use level of the Weak solvent in the preparatory phase that changes the gel compound, can control the porosity, maximum stress or the average pore size of multiporous biological absorbable polymer. In addition, the porous bioabsorbable material that adopts the manufacture method of porous bioabsorbable material of the present invention to make, its average pore size is little, reaches unanimity, and is the compact texture body with large maximum stress. Therefore, although be the porous membrane shape, but has large stress, and its hole (hole) is less, be favourable to preventing that cell from entering, and the aperture of hole (hole) also is homogeneous, has the glucose permeability, thereby be conducive to through materials such as nutrients, as the porous bioabsorbable material of film shape, be exceedingly useful as adherence preventing material particularly therefore.

Claims (10)

1. porous bioabsorbable material is characterized in that, the maximum stress of bioabsorbable material is 3～23MPa, and the porosity is 0.1～82%, and average pore size is 9～34 μ m.

2. porous bioabsorbable material according to claim 1, wherein, the bioresorbable polymer is the copolymer of lactide and caprolactone.

3. porous bioabsorbable material according to claim 1 and 2, wherein, this porous bioabsorbable material is the thin film of 50～600 μ m.

4. porous bioabsorbable material according to claim 3, wherein, this porous bioabsorbable material is the anti-tendon adhering material of the thin film of 50～500 μ m.

5. according to each described porous bioabsorbable material in the claim 1～4, it is characterized in that, this porous bioabsorbable material is made by the following stated: utilization has good solvent compatibility, the bioresorbable polymer and Weak solvent makes this bioresorbable polymer gelization, with the bioresorbable polymer lyophilization of this gelation.

6. the manufacture method of each described porous bioabsorbable material in the claim 1～5, it is characterized in that, the mixed solvent that utilization has good solvent compatibility, the bioresorbable polymer and Weak solvent makes this bioresorbable polymer gelization, with the gelation thing lyophilization of this gelation, make it porous.

7. the manufacture method of bioabsorbable material according to claim 6 is characterized in that, good solvent Shi diox, and Weak solvent is a water.

8. according to the manufacture method of claim 6 or 7 described bioabsorbable materials, wherein, the use level of Weak solvent is 12～40 weight %.

9. according to the manufacture method of each described bioabsorbable material in the claim 6～8, wherein, control the aperture of multiporous biological absorbable polymer by the rate of cooling that changes described gelation thing.

10. according to the manufacture method of each described bioabsorbable material in the claim 6～9, wherein, control the porosity of multiporous biological absorbable polymer by the use level of the Weak solvent in the preparatory phase that changes described gelation thing.

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