CN101397578A - Enzymolysis technique for extraction of stachyose - Google Patents

Abstract

The invention provides an enzymolysis technology for extracting stachyose, which takes Chinese ortichoke fresh base as a raw material, and releases the stachyose from the cell by adding the enzyme solution with the mass volume ratio of 1:8 in the material and through two times of enzymolysis. The optimum enzyme solution selection, proportioning and concentration are as follows: 1.0 to 1.5 percent of cellulase, 0.8 to 1.2 percent of pectinase and 0.6 to 1.0 percent of protease; and the proportioning is carried out to the pectinase, the protease and the cellulose, and the enzymolysis conditions are as follows: the time of enzymolysis time is 80 to 100min, the temperature for enzymolysis of 35 to 45 DEG C, and the pH value for enzymolysis of 4.5 to 5.5.

Description

The zymolysis technique that a kind of stachyose extracts

Technical field:

The present invention relates to stachyose enzymolysis and extraction technology.Specifically relating to the bright base of herbvore silkworm is raw material, the zymolysis technique in the stachyose Enzymatic Extraction technology.Relate to 2 technical fields of enzyme engineering and processing of farm products.

Background technology:

Stachyose (Stachyose) belongs to raffinose and belongs to galactosides irreducibility functional oligose.It can significantly promote bifidus bacillus propagation, is described as " superpower bifidus factor "; In addition, stachyose can also promote the increase of short chain fatty acid in the intestines, reduces pH value in the intestines, keeps microecological balance in the enteron aisle, suppresses the production of cause of disease spoilage organism, and can promote enteron aisle to trace elements absorbed such as calcium, magnesium; It also has concurrently regulates immune, hypoglycemic and reducing blood-fat (cholesterol) function; Also can do the sweeting agent and the nutritive substance of low sugariness.The stachyose physico-chemical property is stable and physiological function is extensive, and safe and reliable through the toxicological experiment confirmation, can be widely used in the fields such as medicines and health protection, food, makeup, beverage, wine, feed class, and potentiality to be exploited is huge.

The major industry raw material that extracts stachyose is Rhizome of Bear's-foot Fern (Chinese artichoke), and Rhizome of Bear's-foot Fern has another name called Chinese artichoke, Myrmeleonmicans Mac Lachlan, artichoke betony, gestumor etc., and all there is plantation on ground such as China Chongqing, Jiangsu, Hebei, Inner Mongol, Shaanxi and Ningxia.Rhizome of Bear's-foot Fern stem tuber spirrillum contains the oligose of about 12%-15% based on stachyose.Extracting the stachyose production method with Rhizome of Bear's-foot Fern has two kinds of natural extract method, Enzymatic Extraction, and it is still at the experimental stage at present that Enzymatic Extraction is used for stachyose production, and the domestic production stachyose generally all adopts the natural extract method.

The extracting method that the plant carbohydrate is commonly used has: hot water extraction, acidleach formulation, alkali extraction, enzyme process etc.Because it is thick that many sugar extract raw cell, wall thickness is difficult to come out from born of the same parents' internal diffusion with the water extraction carbohydrate, needs extracted many times, and it is higher to extract temperature, or the vegetable material grinding is thinner, and the operating time is long, and yield is low, and stachyose water extraction extraction yield is about 40%.Acidleach formulation and alkali extraction make part carbohydrate generation hydrolysis easily, destroy carbohydrate active structure, reduce sugared yield.And select appropriate enzyme for use, and can more leniently plant tissue be decomposed, quicken the release of effective constituent, extraction process is easy, and extraction yield and production efficiency improve.Enzymatic Extraction technology may further comprise the steps: raw material pulverizing, enzymolysis, centrifugal slagging-off, decolouring, desalination, vacuum concentration, spraying drying, eight steps of collection product.Zymolysis technique is the core technology of aqueous enzymatic extraction technology.

Stachyose extracts zymolysis technique research and does not appear in the newspapers.In the extraction of plant carbohydrate, to lentinan, flammulina velutipes, Auricularia polysaccharide, synanthrin, tea polysaccharide, raffinose, Hijiki polysaccharide, astragalus polysaccharides etc., zymolysis technique research is more, and used enzyme mainly contains cellulase, polygalacturonase, papoid, bromeline etc.Studies show that: the best enzyme liquid proportioning that different plant sugar extract is all different with enzymatic hydrolysis condition, but extraction efficiency all has tangible increase.Fu Bo waits (2002) to extract tea polysaccharide cellulase 2.2 μ L/g by force, reaction times 120min, and relative water extraction, the Enzymatic Extraction rate increases by 63.3% and 98.9% respectively.Wen Huiliang etc. (2005) extract raffinose with polygalacturonase concentration 1.8mg/ml, and reaction times 120min, cellulase consumption are 1.5%, hydrolysis time 140min, all can significantly improve extraction yield.With cellulase 1.0%, bromeline 1.0%, polygalacturonase 1.0%, optimum process condition were time 80min when Yu Dongsheng etc. (2001) extracted flammulina velutipes, 50 ℃ of temperature, and pH value 40 improves extraction yield about 50%.

Summary of the invention:

The objective of the invention is to set up with the bright base of Rhizome of Bear's-foot Fern is the zymolysis technique of raw material.

Content of the present invention comprises the selection of (1) enzyme liquid, proportioning and concentration, (2) enzymatic hydrolysis condition.

The selection of content of the present invention (1) enzyme liquid, proportioning and concentration are that following scheme selects one: A cellulase 0.2%-2.0%, proteinase-10 .2%-1.0%; B polygalacturonase 0.2%-1.5%, proteinase-10 .2%-1.5%; C cellulase 0.5%-1.5%, polygalacturonase 0.5%-1.5%; D cellulase 0.2%-2.0%, polygalacturonase 0.2%-1.5%, proteinase-10 .2%-1.5%.Content of the present invention (2) enzymatic hydrolysis condition is enzymolysis time 60-120min, hydrolysis temperature 35-60 ℃, and enzymolysis pH value 3.0-6.5.

The particularly preferred method of the present invention is that the selection of (1) enzyme liquid, proportioning and concentration are cellulase 1.0%-1.5%, polygalacturonase 0.8%-1.2%, proteinase-10 .6%-1.0%; (2) enzymatic hydrolysis condition is enzymolysis time 80-100min, hydrolysis temperature 35-45 ℃, and enzymolysis pH value 4.5-5.5.

The invention has the advantages that: shorten stachyose extraction time, reduce production costs, increase extraction yield 50%-80%, enhance productivity.

Embodiment:

Further illustrate the present invention in the following embodiments, this does not limit the scope of the invention.

Embodiment 1

Rhizome of Bear's-foot Fern originates from Qianjiang District of Chongqing, and after it was cleaned, after airing 1-2 days, measuring its stachyose content with the LPHC method was 12.5g/g.FW.Place in the warehouse in the dress bamboo basket and store.

Take by weighing Rhizome of Bear's-foot Fern 10kg, disperse pulverizer to pulverize with the GSF wet method after, add volume and be in 100 liters the stainless steel enzymatic vessel, get the enzyme liquid 80L for preparing and add in the enzymatic vessel, be 5.0 with the sulfuric acid adjust pH, setting the enzymatic vessel temperature then is 37 ℃, enzymolysis 80min.Enzyme liquid proportioning is a cellulase 1.2%, polygalacturonase 1.2%, proteolytic enzyme 1.0%.Carry out suction filtration behind the enzymolysis, filter residue 3.8kg adds same enzyme liquid 30L enzymolysis once more under similarity condition, behind the suction filtration, and filter residue 0.9kg.After the mixing of secondary enzymolysis filtrate, acquisition stachyose crystallization 0.98kg after decolouring, desalination, vacuum concentration, spraying drying etc., the stachyose yield is 78%.

Embodiment 2

Enzymolysis process is undertaken by embodiment 1, and institute's difference is that enzyme liquid proportioning is a cellulase 1.5%, polygalacturonase 1.0%, proteinase-10 .8%.Enzymolysis time is 100min.The stachyose yield is 76%.

Embodiment 3

Enzymolysis process is undertaken by embodiment 1, and institute's difference is that enzyme liquid proportioning is a cellulase 1.0%, polygalacturonase 1.0%, proteinase-10 .8%.45 ℃ of hydrolysis temperatures, enzymolysis pH value 5.5.Enzymolysis time 100min.The stachyose yield is 81%.

Claims (6)

1. the zymolysis technique that extracts of a stachyose, this technology comprises the selection of (1) enzyme liquid, proportioning and concentration; (2) enzymatic hydrolysis condition.

2. in accordance with the method for claim 1, it is characterized in that with the bright base of Rhizome of Bear's-foot Fern be that raw material extracts stachyose.

3. in accordance with the method for claim 1, it is characterized in that enzyme liquid is chosen as: polygalacturonase, proteolytic enzyme, cellulase.

4, in accordance with the method for claim 1, it is characterized in that enzymatic hydrolysis condition comprises: hydrolysis temperature, enzymolysis time, enzymolysis pH value.

5, in accordance with the method for claim 3, the selection of enzyme liquid, proportioning and concentration are that following scheme selects one: A cellulase 0.2%-2.0%, proteinase-10 .2%-1.0%; B polygalacturonase 0.2%-1.5%, proteinase-10 .2%-1.5%; C cellulase 0.5%-1.5%, polygalacturonase 0.5%-1.5%; D cellulase 0.2%-2.0%, polygalacturonase 0.2%-1.5%, proteinase-10 .2%-1.5%.Content of the present invention (2) enzymatic hydrolysis condition is enzymolysis time 60-120min, hydrolysis temperature 35-60 ℃, and enzymolysis pH value 3.0-6.5.

6, in accordance with the method for claim 4, enzymatic hydrolysis condition is enzymolysis time 60-120min, hydrolysis temperature 35-60 ℃, and enzymolysis pH value 3.0-6.5.