CN101375688A - Salt-resistance formulation for improving salt resistance of wheat and method of use thereof - Google Patents

Salt-resistance formulation for improving salt resistance of wheat and method of use thereof Download PDF

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CN101375688A
CN101375688A CNA200810139345XA CN200810139345A CN101375688A CN 101375688 A CN101375688 A CN 101375688A CN A200810139345X A CNA200810139345X A CN A200810139345XA CN 200810139345 A CN200810139345 A CN 200810139345A CN 101375688 A CN101375688 A CN 101375688A
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wheat
salt
pref
salt pref
seed
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CN101375688B (en
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孔令安
王法宏
李升东
司纪升
冯波
张宾
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CROP Research Institute of Shandong Academy of Agricultural Sciences
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Abstract

A salt-resistant preparation for improving the salt resistance of the wheat and an application method thereof belong to the technical field of improving the salt resistance of the wheat to increase the wheat production. The method comprises the following steps: 1) soaking in the salt resistance preparation, wherein, with the overall weight being 1kg , the final composite ingredients are 3-4g of Ca (NO3)2, 1.2-1.5g of KH2PO4, 1.5-2g of KI, 0.35g of MnSO4, 10mg of Na2SeO3, 40-60mg of cytokinin, and water in balancing amount; 2) spraying the salt resistant preparation on the surfaces of leaves, wherein, with the overall weight being 1kg, the final composite ingredients are 1-1.2g of Ca (NO3)2, 0.25-0.3g of KH2PO4, 0.35g of KI, 0.1g of MnSO4, 2mg of Na2SeO3, 2-10mg of cytokinin, and water in balancing amount. Wheat seeds are soaked in the salt resistant preparation for one time before being planted, and the salt resistant preparation is sprayed on the surfaces of the leaves after the fifteen days of wheat seedlings turn green in the spring of the next year. The invention has the advantages of significantly improving the ratio of emergence of wheat seeds under high salinity, promoting the growing development of wheat plants, increasing the production of the wheat by 15-20 percent, and being suitable for the soil with salt contents being 0.3 percent.

Description

A kind of anti-salt pref and application method thereof that improves the wheat salt-resistance
Technical field
The present invention relates to improve the wheat salt-resistance to improve the technical field of wheat yield, the particularly a kind of anti-salt pref of wheat salt-resistance and application method of this anti-salt pref of improving.
Technical background
Making full use of of salinization soil is to promote agro based economic development, the important channel of improving agroecological environment.There are salination and secondary salinization soil in China more than 4,000 ten thousand hectares, accounts for about 1/10 of China arable land.Large-area salination soil has had a strong impact on grain yield, has become a very important limiting factor of China's agricultural production.Along with the increase of population and the minimizing of arable area, to the year two thousand thirty China's total output of grain only reach hundred million tons of 6.4-7, promptly grain yield must could satisfy the grain demand of China substantially improving more than 50% on the level at present.Thereby in today that freshwater resources worsening shortages, land area reduce gradually, development and use salination soil has become a present important research target, has become the key subjects that press for solution now.
To studies show that of plant anti-salt mechanism: soil salt too much can suppress plant growing, reduces crop yield.Salinity too much produces the main cause that injures to plant two aspects: the one, and ion is poisoned and nutritional deficiency; The 2nd, physiological drought.This two aspect is all relevant with the integrality of too much salt ion destruction cell membrane.Can make the plant salt evil be able to part by rational soil and water management and chemical modifying measure and alleviate, but cost is too high; Though the breeding scholar is by cultivating and screening salt tolerant crop kind, obtained certain achievement, utilize a kind of new foreign DNA to import means as Chinese patent CN1230593 is disclosed, improve the salt-resistance of recipient plant, but see still not very good from present present situation.Wheat is the second largest cereal crops of China, and salinity is coerced the comparison sensitivity.Because its vegetative period is long, the autumn that arid just short of rain, soil transpiration rate of winter wheat sowing phase is big, the soil salinization rises, and the seedling fast growing period accumulation of salt in the surface soil serious spring just, therefore, from regulation and control plant physiology active mechanism angle, the saline-alkaline tolerance that utilizes the chemical control means to improve wheat is one of important channel of improving wheat yield.
Summary of the invention
The purpose of this invention is to provide a kind of anti-salt pref that improves the wheat salt-resistance, this anti-salt pref can improve the tolerance of wheat to salinity effectively, wheat can be grown in the soil of salt content 0.3% and obtains higher output.
The present invention also provides the application method of this anti-salt pref.
The technical scheme that technical solution problem of the present invention is taked is:
A kind of anti-salt pref that improves the wheat salt-resistance is characterized in that, soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation, seed soaks anti-salt pref before the described wheat cultivation, in the gross weight is 1kg, and its composition finally is Ca (NO 3) 23-4g, KH 2PO 41.2-1.5g, KI 1.5-2g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 40-60mg, surplus is a water; The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and its composition finally is Ca (NO 3) 21-1.2g, KH 2PO 40.25-0.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 2-10mg, surplus is a water.
The anti-salt pref of described raising wheat salt-resistance can also be: seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and its composition finally is Ca (NO 3) 23.5g, KH 2PO 41.3g, KI 1.8g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 50mg, surplus is a water; The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and its composition finally is Ca (NO 3) 21.2g, KH 2PO 40.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 6mg, surplus is a water.
The described basic element of cell division is the 6-benzylaminopurine.
The described basic element of cell division is a zeatin, chemical name: 6-is trans-and 4-hydroxy-3-methyl-but-2-ene base aminopurine.
The described basic element of cell division is a 6-furfuryl group aminopurine.
Anti-salt pref can be stored with 100 times of concentrates, directly is made into 100 times of solution during formulated product, and the time spent is diluted to ultimate density again.Also can be directly with solid form preparation and storage, the time spent is diluted to ultimate density again.
The application method that improves the anti-salt pref of wheat salt-resistance is:
Soak 1 time before the sowing, before soaking seed before the wheat cultivation being soaked anti-salt pref adds clear water and is diluted to ultimate density, wheat seed with election contest soaked 5-6 hour in anti-salt pref then, and seed soaks anti-salt pref by the anti-salt pref input of every 4000ml 10kg wheat seed before wheat seed and the wheat cultivation; At room temperature dry after the immersion to being fit to sowing humidity and get final product, preserve as the need long period, seed should dry to moisture below 14%, and in preservation below 15 ℃;
Wheat seedling phase foliage-spray is used: spring in next year, wheat seedling began foliage-spray 2-3 time after turning green 15 days, each 15-20 days at interval; Before spraying, the anti-salt pref of wheat seedling phase foliage-spray is diluted to ultimate density with clear water by a certain percentage, the amount of spraying is at every mu of 12.5L; The time of spraying should be selected at dusk, helps preparation and fully absorbs.
The component that the anti-salt pref of the present invention is selected for use is not merely as nutritive element, and main is: Ca (NO 3) 2With KH 2PO 4After being dissolved in water, can provide the slightly acidic environment of a part, be easy to seed germination (the suitableeest sprouting Acidity of Aikalinity of wheat is pH6.4 ± 0.2) for wheat seeds sprouting; KI and Na 2SeO 3I wherein and Se itself promptly is active oxygen scavenger, can remove reactive oxygen species (the Reactive oxygen species that wheat cell produces owing to salt stress; ROS) and in the seed germination process owing to cell membrane disturbance (perturbations), plant the reactive oxygen species that skin breaks and produces with respiration; In addition, KI and KH 2PO 4Can also promote plant cell to K +Absorption, regulate K in the cell +/ Na +Ratio, and then the ionic equilibrium in the adjusting cell, K nutrition is deficient in the plant cell under the alleviation salt stress lacks.Ca (NO 3) 2, MnSO 4, Na 2SeO 3Can improve membrane protective enzymic activitys such as SOD, POD, CAT in the plant cell, the protection cell structure keeps cellular physiological activity; Ca 2+Be that wheat root growth under the salt stress, growth and the generation of root hair are necessary with growth; NO 3 -Can induce nitrate reductase activity, thereby produce adverse circumstance signal factor NO, improve plant resistance.The basic element of cell division of selecting for use in the anti-salt pref of the present invention (cytokinin) is except that having conventional effect, in anti-salt pref, also have following specific function: the one, the basic element of cell division can be induced wheat cell generation glycinebetaine under the salt stress, plays important osmotic adjustment effect in cell; The 2nd, the basic element of cell division is induced nitrate reductase activity, promotes absorption and the utilization of cell to N, regulates proteometabolism in the cell; The 3rd, the basic element of cell division can be coerced down the ABA that plant cell produces with salinity and be played antagonism, suppresses the generation of reactive oxygen species.Therefore, the selected component of the present invention is not simple combination, but by its synergy, makes the saline-alkaline tolerance of wheat plant reach the strongest state.
Beneficial effect of the present invention is:
1, the present invention is from ionic equilibrium, osmotic adjustment, oxygen scavenging activity, the activation of membrane protective enzyme, nutritive element demand equal angles, multi level hoisting wheat saline-alkaline tolerance.
2, the present invention can improve the tolerance of wheat to salinity effectively, wheat can be grown in the soil of salt content 0.3% and obtains higher output.Adopt the present invention can significantly improve salinity and coerce down wheat seed emergence rate, growth of seedling speed, leaf photosynthesis speed, promotion wheat plant grow, and make wheat yield improve 15-20%; Adopt the present invention to make the saturating relatively property reduction by 28% of wheat plant cell membrane, SOD is active to improve 55%, and POD raising 35%, CAT improve 40%, K +/ Na +Improve 20-30%; Adopt the present invention to improve the tolerance of wheat effectively, wheat can be grown in the soil of salt content 0.3% and obtain higher output salinity.See Table 1, table 2 be 2006/2007 and 2007/2008 year in Wei Bei farm, Shandong Province the result of the test of (soil salt content is 0.3%).
Wheat growth biological character contrast (2 annual mean) under processing of table 1 anti-salt agent and the collating condition
Figure A200810139345D00071
Wheat yield and output constitute contrast (2 annual mean) under processing of table 2 anti-salt agent and the collating condition
Figure A200810139345D00072
3, composition of the present invention is simple, is easy to preparation, and cost is low.
Embodiment
Embodiment 1
A kind of anti-salt pref that improves the wheat salt-resistance soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 23g, KH 2PO 41.2g, KI 1.5g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 40mg, surplus is a water;
The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 21g, KH 2PO 40.25g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 2mg, surplus is a water.The described basic element of cell division is the 6-benzylaminopurine.
Directly be made into 100 times of solution during formulated product.
The application method that improves the anti-salt pref of wheat salt-resistance is:
Soak 1 time before the sowing, before soaking seed before the wheat cultivation being soaked anti-salt pref adds clear water and is diluted to ultimate density, wheat seed with election contest soaked 5-6 hour in anti-salt pref then, and seed soaks anti-salt pref by the anti-salt pref input of every 4000ml 10kg wheat seed before wheat seed and the wheat cultivation; At room temperature dry after the immersion to being fit to sowing humidity and get final product, preserve as the need long period, seed should dry to moisture below 14%, and in preservation below 15 ℃;
Wheat seedling phase foliage-spray is used: began foliage-spray 2 times after spring in next year, wheat seedling was turned green 15 days, each 15 days at interval; Before spraying, the anti-salt pref of wheat seedling phase foliage-spray is diluted to ultimate density with clear water by a certain percentage, the amount of spraying is at every mu of 12.5L; The time of spraying should be selected at dusk, helps preparation and fully absorbs.
Embodiment 2
A kind of anti-salt pref that improves the wheat salt-resistance soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 24g, KH 2PO 41.5g, KI 2g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 60mg, surplus is a water;
The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 21.2g, KH 2PO 40.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 10mg, surplus is a water.The described basic element of cell division is the 6-benzylaminopurine.
The described basic element of cell division is zeatin (chemical name: 6-trans-4-hydroxy-3-methyl-but-2-ene base aminopurine).
Wheat seedling phase foliage-spray is used: began foliage-spray 3 times after spring in next year, wheat seedling was turned green 15 days, each 20 days at interval.
All the other are with embodiment 1.
Embodiment 3
A kind of anti-salt pref that improves the wheat salt-resistance soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and its composition finally is Ca (NO 3) 23.5g, KH 2PO 41.3g, KI 1.8g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 50mg, surplus is a water; The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and its composition finally is Ca (NO 3) 21.2g, KH 2PO 40.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 6mg, surplus is a water.
The described basic element of cell division is a 6-furfuryl group aminopurine.
Wheat seedling phase foliage-spray is used: began foliage-spray 3 times after spring in next year, wheat seedling was turned green 15 days, each 20 days at interval.
All the other are with embodiment 1.
Embodiment 4
A kind of anti-salt pref that improves the wheat salt-resistance soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 24g, KH 2PO 41.2g, KI 1.8g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 60mg, surplus is a water;
The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and it finally consists of, Ca (NO 3) 21.2g, KH 2PO 40.25g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 8mg, surplus is a water.The described basic element of cell division is the 6-benzylaminopurine.
Anti-salt pref is stored with solid form.
Wheat seedling phase foliage-spray is used: began foliage-spray 3 times after spring in next year, wheat seedling was turned green 15 days, each 18 days at interval.
All the other are with embodiment 1.

Claims (6)

1, a kind of anti-salt pref that improves the wheat salt-resistance is characterized in that, soaks anti-salt pref and wheat seedling phase foliage-spray is formed with anti-salt pref by seed before the wheat cultivation,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and its composition finally is Ca (NO 3) 23-4g, KH 2PO 41.2-1.5g, KI 1.5-2g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 40-60mg, surplus is a water;
The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and its composition finally is Ca (NO 3) 21-1.2g, KH 2PO 40.25-0.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 2-10mg, surplus is a water.
2, a kind of anti-salt pref that improves the wheat salt-resistance according to claim 1 is characterized in that,
Seed soaks anti-salt pref before the described wheat cultivation, is 1kg in the gross weight, and its composition finally is Ca (NO 3) 23.5g, KH 2PO 41.3g, KI 1.8g, MnSO 40.35g, Na 2SeO 310mg, basic element of cell division 50mg, surplus is a water;
The anti-salt pref of described wheat seedling phase foliage-spray is 1kg in the gross weight, and its composition finally is Ca (NO 3) 21.2g, KH 2PO 40.3g, KI 0.35g, MnSO 40.1g, Na 2SeO 32mg, basic element of cell division 6mg, surplus is a water.
3, a kind of anti-salt pref that improves the wheat salt-resistance according to claim 1 is characterized in that the described basic element of cell division is the 6-benzylaminopurine.
4, a kind of anti-salt pref that improves the wheat salt-resistance according to claim 1 is characterized in that the described basic element of cell division is a zeatin, chemical name: 6-is trans-and 4-hydroxy-3-methyl-but-2-ene base aminopurine.
5, a kind of anti-salt pref that improves the wheat salt-resistance according to claim 1 is characterized in that, the described basic element of cell division is a 6-furfuryl group aminopurine.
6, improve the application method of the anti-salt pref of wheat salt-resistance according to claim 1, it is characterized in that:
Soak 1 time before the sowing, before soaking seed before the wheat cultivation being soaked anti-salt pref adds clear water and is diluted to ultimate density, wheat seed with election contest soaked 5-6 hour in anti-salt pref then, and seed soaks anti-salt pref by the anti-salt pref input of every 4000ml 10kg wheat seed before wheat seed and the wheat cultivation; At room temperature dry after the immersion to being fit to sowing humidity and get final product, preserve as the need long period, seed should dry to moisture below 14%, and in preservation below 15 ℃;
Wheat seedling phase foliage-spray is used: spring in next year, wheat seedling began foliage-spray 2-3 time after turning green 15 days, each 15-20 days at interval; Before spraying, the anti-salt pref of wheat seedling phase foliage-spray is diluted to ultimate density with clear water by a certain percentage, the amount of spraying is at every mu of 12.5L; The time of spraying should be selected at dusk, helps preparation and fully absorbs.
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102670615A (en) * 2012-01-04 2012-09-19 河南科技大学 Application of 6-benzyl aminopurine compound in terms of preparation of composition for suppressing oxidative injury to brain tissues
CN105621616A (en) * 2014-11-27 2016-06-01 四川农业大学 Method for obtaining plant capable of efficiently absorbing nitrate
CN104430517B (en) * 2014-11-24 2017-07-07 广东省农业科学院农业资源与环境研究所 A kind of sweet basil salt resistance seed soaking agent and preparation method thereof
CN109938044A (en) * 2019-03-20 2019-06-28 安徽农业大学 A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method
CN109970497A (en) * 2019-04-04 2019-07-05 成都大学 A kind of salt stress-resistant agent and preparation method thereof for wheat crops
CN116250479A (en) * 2023-02-15 2023-06-13 青岛农业大学 Screening method of novel salt-tolerant selenium-rich wheat germplasm

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JP2896963B2 (en) * 1994-11-28 1999-05-31 株式会社コスモ総合研究所 Plant salt tolerance improver
CN1290483A (en) * 1999-09-30 2001-04-11 山东师范大学 Anti-salt drought resistant agent for plant and its using method
CN1319457C (en) * 2005-02-06 2007-06-06 山东省农业科学院土壤肥料研究所 Plant salt-resisting agent, and its preparing and use method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102670615A (en) * 2012-01-04 2012-09-19 河南科技大学 Application of 6-benzyl aminopurine compound in terms of preparation of composition for suppressing oxidative injury to brain tissues
CN102670615B (en) * 2012-01-04 2013-12-25 河南科技大学 Application of 6-benzyl aminopurine compound in terms of preparation of composition for suppressing oxidative injury to brain tissues
CN104430517B (en) * 2014-11-24 2017-07-07 广东省农业科学院农业资源与环境研究所 A kind of sweet basil salt resistance seed soaking agent and preparation method thereof
CN105621616A (en) * 2014-11-27 2016-06-01 四川农业大学 Method for obtaining plant capable of efficiently absorbing nitrate
CN109938044A (en) * 2019-03-20 2019-06-28 安徽农业大学 A kind of medicament that Wheat in Grain Filling Stage high temperature damage is effectively relieved and method
CN109970497A (en) * 2019-04-04 2019-07-05 成都大学 A kind of salt stress-resistant agent and preparation method thereof for wheat crops
CN116250479A (en) * 2023-02-15 2023-06-13 青岛农业大学 Screening method of novel salt-tolerant selenium-rich wheat germplasm

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