CN101368174B - Levulose valine oxidizing enzyme of high activity - Google Patents
Levulose valine oxidizing enzyme of high activity Download PDFInfo
- Publication number
- CN101368174B CN101368174B CN2008101663406A CN200810166340A CN101368174B CN 101368174 B CN101368174 B CN 101368174B CN 2008101663406 A CN2008101663406 A CN 2008101663406A CN 200810166340 A CN200810166340 A CN 200810166340A CN 101368174 B CN101368174 B CN 101368174B
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- CN
- China
- Prior art keywords
- enzyme
- activity
- valine
- levulose
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000790 Enzymes Proteins 0.000 title claims abstract description 43
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 43
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 title claims abstract description 31
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 239000004474 valine Substances 0.000 title claims abstract description 28
- 230000000694 effects Effects 0.000 title claims abstract description 25
- 229960002737 fructose Drugs 0.000 title claims description 24
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 title claims description 21
- 230000001590 oxidative effect Effects 0.000 title claims description 21
- 150000001413 amino acids Chemical group 0.000 claims abstract description 11
- 239000002773 nucleotide Substances 0.000 claims abstract description 4
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 4
- 239000002157 polynucleotide Substances 0.000 claims description 2
- 102000040430 polynucleotide Human genes 0.000 claims description 2
- 108091033319 polynucleotide Proteins 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 12
- 230000035772 mutation Effects 0.000 abstract description 12
- 108090000623 proteins and genes Proteins 0.000 abstract description 11
- 229930091371 Fructose Natural products 0.000 abstract description 10
- 239000005715 Fructose Substances 0.000 abstract description 10
- 238000002360 preparation method Methods 0.000 abstract description 7
- 238000006243 chemical reaction Methods 0.000 abstract description 6
- 241000745841 Arthrobacter sp. 2-4-1 Species 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 5
- 238000012408 PCR amplification Methods 0.000 abstract description 4
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 230000035484 reaction time Effects 0.000 abstract description 3
- 230000014509 gene expression Effects 0.000 abstract description 2
- 102000004316 Oxidoreductases Human genes 0.000 abstract 7
- 108090000854 Oxidoreductases Proteins 0.000 abstract 7
- 241000588724 Escherichia coli Species 0.000 abstract 2
- LOUPRKONTZGTKE-WZBLMQSHSA-N Quinine Chemical compound C([C@H]([C@H](C1)C=C)C2)C[N@@]1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-WZBLMQSHSA-N 0.000 abstract 2
- 235000001258 Cinchona calisaya Nutrition 0.000 abstract 1
- LOUPRKONTZGTKE-UHFFFAOYSA-N cinchonine Natural products C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-UHFFFAOYSA-N 0.000 abstract 1
- 229960000948 quinine Drugs 0.000 abstract 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 4
- 238000006555 catalytic reaction Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 241000186216 Corynebacterium Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 102000003992 Peroxidases Human genes 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 230000009257 reactivity Effects 0.000 description 3
- 238000006479 redox reaction Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 125000000250 methylamino group Chemical group [H]N(*)C([H])([H])[H] 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000004904 shortening Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- ZTQGWROHRVYSPW-UHFFFAOYSA-N 3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonic acid Chemical compound OS(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 ZTQGWROHRVYSPW-UHFFFAOYSA-N 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101710176276 SSB protein Proteins 0.000 description 1
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229940049547 paraxin Drugs 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000008057 potassium phosphate buffer Substances 0.000 description 1
- 230000009465 prokaryotic expression Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- IRQRBVOQGUPTLG-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 IRQRBVOQGUPTLG-UHFFFAOYSA-M 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008101663406A CN101368174B (en) | 2008-09-23 | 2008-09-23 | Levulose valine oxidizing enzyme of high activity |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2008101663406A CN101368174B (en) | 2008-09-23 | 2008-09-23 | Levulose valine oxidizing enzyme of high activity |
Publications (2)
Publication Number | Publication Date |
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CN101368174A CN101368174A (en) | 2009-02-18 |
CN101368174B true CN101368174B (en) | 2011-04-27 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN2008101663406A Active CN101368174B (en) | 2008-09-23 | 2008-09-23 | Levulose valine oxidizing enzyme of high activity |
Country Status (1)
Country | Link |
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CN (1) | CN101368174B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114634918B (en) * | 2022-05-19 | 2022-10-28 | 鲁东大学 | D-amino acid oxidase mutant, engineering bacteria and application |
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2008
- 2008-09-23 CN CN2008101663406A patent/CN101368174B/en active Active
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Publication number | Publication date |
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CN101368174A (en) | 2009-02-18 |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C53 | Correction of patent of invention or patent application | ||
CB03 | Change of inventor or designer information |
Inventor after: Zou Bingde Inventor before: Zou Bingde Inventor before: Jiang Yunfei |
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Free format text: CORRECT: INVENTOR; FROM: ZOU BINGDE JIANG YUNFEI TO: ZOU BINGDE |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C56 | Change in the name or address of the patentee |
Owner name: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD. Free format text: FORMER NAME: MEIKANG BIOTECH CO., LTD., NINGBO |
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CP01 | Change in the name or title of a patent holder |
Address after: 315100 Ningbo science and technology zone, Yinzhou City, Zhejiang, Ma Ma Industrial Park Road, No. 358 Patentee after: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Address before: 315100 Ningbo science and technology zone, Yinzhou City, Zhejiang, Ma Ma Industrial Park Road, No. 358 Patentee before: Ningbo Medical System Biotechnology Co.,Ltd. |
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CP03 | Change of name, title or address |
Address after: 315104 Zhejiang city of Ningbo province Yinzhou District Qiming Road No. 299 Patentee after: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Address before: 315100 Ningbo science and technology zone, Yinzhou City, Zhejiang, Ma Ma Industrial Park Road, No. 358 Patentee before: NINGBO MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. |
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CP03 | Change of name, title or address | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20090218 Assignee: Ningbo Shengyuan Biotechnology Co., Ltd. Assignor: MEDICAL SYSTEM BIOTECHNOLOGY Co.,Ltd. Contract record no.: X2022330000361 Denomination of invention: Highly active fructose-valine oxidase Granted publication date: 20110427 License type: Common License Record date: 20220809 |
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EE01 | Entry into force of recordation of patent licensing contract |