CN101366831B - Rhubarb polyoses, preparation method, medicament composition and uses - Google Patents
Rhubarb polyoses, preparation method, medicament composition and uses Download PDFInfo
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- CN101366831B CN101366831B CN2007101439694A CN200710143969A CN101366831B CN 101366831 B CN101366831 B CN 101366831B CN 2007101439694 A CN2007101439694 A CN 2007101439694A CN 200710143969 A CN200710143969 A CN 200710143969A CN 101366831 B CN101366831 B CN 101366831B
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Abstract
The invention relates to rhubarb polysaccharides, wherein the rhubarb polysaccharides comprises rhubarb polysaccharides with the range of molecular weight section of between 0.2 and 0.3x10<5> and between 1 and 1.2x10 <5>. The rhubarb polysaccharides are prepared from the traditional Chinese medicine of rhubarb by the processes of ultrasonic extraction, ultrafiltration separation concentration, and purification. The rhubarb polysaccharides can be made into a rhubarb polysaccharides medicine composition together with a medically acceptable carrier. The medicament type which can be made into comprises freeze-dried powder injection, powder-injection, injection solution, orally disintegrating tablets, dispersible tablets, effervescent tablets, controlled release tablets, sustained-release tablets, common film-coated tablets, capsule, granular formulation, dropping pills, and the like. The rhubarb polysaccharides medicine composition is used for treating diabetes, resisting tumor, resisting aging and improving the immunity.
Description
Technical field
The present invention relates to the method for preparing of a kind of rhubarb polyoses and this rhubarb polyoses and the application of other pharmaceutical composition and pharmaceutical composition, belong to the field of Chinese medicines.
Background technology
Radix Et Rhizoma Rhei is the dry root and rhizome of polygonum rheum palmatum Rheum palmatum L., Rheum tanguticum Rheum tanguticumMaxim.Ex Balf. or Rheum officinale Rheum officinale Baill.; Main product is in China Qinghai, Gansu, Ningxia, Inner Mongol, Sichuan, Guizhou, Yunnan and other places; Through clinical practice abundant over more than 2,000 year, Radix Et Rhizoma Rhei has demonstrated range of application and reliable curative effect widely.The Radix Et Rhizoma Rhei bitter in the mouth, cold in nature, return stomach, spleen, large intestine, liver, pericardium channel, have effects such as purging heat to relax the bowels, removing toxicity for eliminating carbuncles, clots absorbing stimulate the menstrual flow, removing damp-heat, but also cooling blood for hemostasis.Be usually used in that the gastrointestinal excess-heat is stagnant, constipation, abdominal part distension, pain with tenderness; Even hyperpyrexia, unconsciousness and delirium,, pathopyretic ulcer, sudden and violent acute conjunctivitis pain, aphtha of the mouth and tongue, gingivitis; Menstruation apolipsis, postpartum stagnation, lump in the abdomen; And traumatic injury, swelling and pain due to blood stasis, also be used for jaundice, dysuria, the constipation with dry stool of stagnation of damp-heat, the haematemesis of injury of blood collaterals by heat, epistaxis, have blood in stool, metrorrhagia, leucorrhea with red and white discharge.
Modern scientific method has also carried out extremely going deep into extensive studies to Radix Et Rhizoma Rhei, and it is eight big types of main over one hundred kind of organic and inorganic compositions that known so far Radix Et Rhizoma Rhei contains with the anthraquinone derivatives.Pharmacological research closes and shows, Radix Et Rhizoma Rhei and preparation thereof have rush down down, effects such as antiinflammatory, antibiotic, antiviral, inhibition conk, antitumor, anti-psoriasis, function of gallbladder promoting.These basic research have been done scientific explarnation for many-sided curative effect clinically undoubtedly; During modern times were clinical, Radix Et Rhizoma Rhei can be used for treating epidemic cerebrospinal meningitis, lobar pneumonia, acute biliary infection, acute parotitis, acute appendicitis, Fiedeler's disease type hepatitis, acute enteritis, bacillary dysentery, digestive tract hemorrhage, pharyngolaryngitis, gingival abscess, dermatitis, eczema, gonorrhea, herpes zoster etc.Radix Et Rhizoma Rhei so pharmacological effect effect widely is mainly oral and obtain with traditional Chinese medical science conventional formulation one water decoction, is necessary to do further research as the water soluble polysaccharide composition of one of water solublity main constituent.
The water solublity main constituent rhubarb polyoses of Radix Et Rhizoma Rhei is one of focus of research at present, and the component of polysaccharide is separated the general gel column that adopts and separated, and rhubarb polyoses is prone to make gel aging, and cost is higher, therefore is unsuitable for big production.At present, the pharmaceutical formulation of the rhubarb polyoses finished product that also do not go on the market.Along with to the going deep into of the research of rhubarb polyoses, find that rhubarb polyoses has antidiabetic effect, obviously blood sugar lowering and improve serum insulin levels.Rhubarb polyoses also has the effect of antitumor, defying age, enhancing immunity.The diabetics of China has surpassed 1,200 ten thousand people, and along with the raising and the aged tendency of population of economic development, living standards of the people, the onset diabetes rate has the continuation ascendant trend.Modern society, environmental pollution is serious, and stress is big, also strengthens the concern of people to diseases prevention and treatment such as antitumor, defying age, enhancing immunity.The extraction and separation process of research rhubarb polyoses makes it to be suitable for industrialized great production, reduces cost, and processes pharmaceutical formulation, is applied to clinically, is the problem that is worth research.
Publication number is entitled as for CN 1395937A in the patent application of " rhubarb polyoses application in preparation treatment inflammatory bowel medicine and preparation method thereof " mentions the application of rhubarb polyoses in preparation treatment inflammatory bowel medicine.The molecular weight fragment scope of rhubarb polyoses wherein is (6-8) * 10
5In this application, also mentioned the method for preparing of the different rhubarb polyoses of molecular weight.Rhubarb polyoses in this application is used to treat inflammatory bowel, and good effect is arranged.
On " Shanghai laboratory animal science " magazine of 2004 the 24th phases, delivered and be entitled as " rhubarb polyoses is to the effect of several indexs such as diabetic mice serum lipids " literary composition; Mention rhubarb polyoses in the literary composition and had the triglyceride that reduces in the serum; T-CHOL, low density lipoprotein, LDL, the hdl level of the serum that can also raise simultaneously; Thereby the generation of rhubarb polyoses to diabetes complicated angiopathy is described, certain preventive effect is arranged.
In " medicine biotechnology " magazine of publishing in JIUYUE, 2002, delivered and be entitled as " research of rhubarb polyoses separation and purification and function thereof " literary composition, also mentioned the function that rhubarb polyoses has tangible blood sugar lowering and improves serum insulin levels in the literary composition.
Have the treatment diabetes although announced rhubarb polyoses in the prior art; And can be used for the effect of antitumor, defying age, enhancing immunity; But explanation does not adopt the rhubarb polyoses of how many molecular weight ranges can reach good therapeutic effect; And the method that how to obtain the rhubarb polyoses of this molecular weight ranges, if therefore adopt when the rhubarb polyoses of definite molecular weight ranges does not carry out treatment and health, it is not obvious to certainly exist therapeutic effect; The consumption of Radix Et Rhizoma Rhei is big, the tangible problem of side effect.
Summary of the invention
One of the object of the invention just is to develop a kind of rhubarb polyoses, and the method for preparing of this rhubarb polyoses, because the molecular weight of the rhubarb polyoses among the present invention is to confirm, so the rhubarb polyoses among the present invention can be used for industrialized great production.
Another object of the present invention is to provide a kind of by the rhubarb polyoses pharmaceutical composition of this rhubarb polyoses preparation and the purposes of this pharmaceutical composition.
One aspect of the present invention provides a kind of rhubarb polyoses, and wherein, this rhubarb polyoses comprises that the molecular weight fragment scope is 0.2～0.3*10
5With 1.0～1.2*10
5Rhubarb polyoses.
Another aspect of the present invention provides a kind of rhubarb polyoses, and wherein, this rhubarb polyoses comprises that the molecular weight fragment scope of 2-8 part volume is 0.2～0.3*10
5Rhubarb polyoses and the molecular weight fragment of 3-12 part volume be 1.0～1.2*10
5Rhubarb polyoses.
Another aspect of the present invention provides a kind of method for preparing rhubarb polyoses, wherein, may further comprise the steps:
A, with the roots and stems of rhubarb clean dry, be ground into coarse grain;
B, with described coarse grain with pure water supersound extraction 3 times, filter merging filtrate;
C, described filtrating is dammed with ultrafiltration, collected molecular weight and be 2～30,000 medicinal liquid;
The method collection molecular weight of d, reuse ultrafiltration is 10～120,000 medicinal liquid;
E, be 10～120,000 combining medicine of 2～30,000 medicinal liquid and 3-12 part volume, concentrate the medicinal liquid after obtaining concentrating the molecular weight of described 2-8 part volume;
F, the medicinal liquid after described concentrate use ethanol precipitation, filtering residue is removed in filtration, obtains the rhubarb polyoses bullion; In the ethanol precipitation process, preferably concentrate is added the dehydrated alcohol that 1-5 doubly measures, stirred 10-60 minute, placed 5-24 hour, (wherein whenever stirred 10-60 minute, stir 2-12 time altogether) at a distance from 2 hours, filtering residue is got in centrifugal filtration, gets the rhubarb polyoses bullion;
G, with the described rhubarb polyoses bullion of dissolved in distilled water, and remove protein wherein, obtain rhubarb polyoses dissolving crude product liquid;
H, in described rhubarb polyoses dissolving crude product liquid, add dehydrated alcohol, deposition is filtered; Remove filtering residue, repeat above-mentioned operation, up to the colour stable of described rhubarb polyoses dissolving crude product liquid; With the aeration-drying of described rhubarb polyoses dissolving crude product liquid, obtain rhubarb polyoses again.Carry out sedimentation and filtration at adding dehydrated alcohol in rhubarb polyoses dissolving crude product liquid, remove in the operation of filtering residue, along with the increase of number of operations; The color of the rhubarb polyoses dissolving crude product liquid that obtains shoals gradually; Gradually become light brown from initial dark brown, become again faint yellow, up to repeatedly repeating aforesaid operations; The color kept stable of the rhubarb polyoses dissolving crude product liquid that obtains no longer changes.
Another aspect of the present invention provides the another kind of method for preparing rhubarb polyoses, wherein after described step b, pure water is extracted the residual filtering residue reuse pure water in back extract 2～3 times, and described pure water is 1～4 times of described filtering residue weight.
Another aspect of the present invention provides the another kind of method for preparing rhubarb polyoses; Wherein in step b, adopt supersound extraction; Step c adopts Hollow Fiber Ultrafiltration to hold back, and adopts the sevag method to remove deproteinize in described step g, also can adopt to add trichloroacetic acid removal protein; In order to reduce Protein content, this method 3-4 time repeatedly as far as possible.
Another aspect of the present invention provides another to prepare the method for rhubarb polyoses; Wherein in step a; Described roots and stems of rhubarb is ground into 10-30 purpose coarse grain, and in described step b, described pure water is 2-5 a times of described coarse-grained weight; In described step e, the liquor strength after concentrating is 0.8～0.2g/ml.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, comprising rhubarb polyoses and pharmaceutically acceptable carrier.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, and rhubarb polyoses pharmaceutical composition wherein comprises: the carrier of the rhubarb polyoses of 1 weight portion and 0.5-200 weight portion.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, and carrier wherein is one or more the combination in mannitol, sorbitol, dextran, glucose, lactose, sucrose, sodium chloride, citric acid, sodium bicarbonate, hypromellose, low-substituted hydroxypropyl cellulose, crospolyvinylpyrrolidone, polyvinylpyrrolidone, microcrystalline Cellulose, poloxamer, tween 80, carboxymethyl starch sodium, pregelatinized Starch, starch, silicon dioxide, xylitol, Macrogol 4000-6000, beta-schardinger dextrin-, cross-linking sodium carboxymethyl cellulose, carboxymethylcellulose calcium or the acrylic resin.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, and pharmaceutical composition wherein is the pharmaceutical composition of gastrointestinal tract, injection or respiratory tract administration.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, and pharmaceutical composition wherein has following dosage form: injectable powder, capsule, injection, drop pill, dispersant, tablet, oral agents, nasal drop.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition, and the dosage form of pharmaceutical composition wherein is lyophilized injectable powder, tablet, injection.
Another aspect of the present invention provides a kind of rhubarb polyoses pharmaceutical composition; Tablet wherein is dispersible tablet, disintegrating tablet, release sheet, controlled release tablet, effervescent tablet, thin membrane coated tablet; Capsule is hard capsule, slow releasing capsule, controlled release capsule, soft capsule, and oral agents is oral cavity disintegration tablet, dispersible tablet, granule, effervescent granule.
Another aspect of the present invention provides a kind of pharmaceutical composition of rhubarb polyoses to be used to treat diabetes, and can be used for antitumor, defying age, enhancing immunity.
The rhubarb polyoses that this method makes, purity can pass through sulphuric acid-phynol method and detect the content of polysaccharide and adopt sulphuric acid-carbazole method to survey the content of alduronic acid, and adopt the Coomassie brilliant blue colorimetry to survey protein content greater than 90%.
The present invention is to modern a kind of trial of Chinese medicine and innovation, because Chinese medicine can only be qualitative, and but can't be quantitative.For rhubarb polyoses, only have that applied rhubarb polyoses has adopted the molecular weight fragment scope to be (6-8) * 10 in the preparation treatment inflammatory bowel medicine with therapeutic effect
5Rhubarb polyoses.Yet rhubarb polyoses in the treatment diabetes; Do not announce that in existing file the rhubarb polyoses that adopts how many molecular weight can reach the effect of treatment; In the present invention is to accomplish: in a rhubarb polyoses product quantitatively; The rhubarb polyoses that promptly in the preparation process of rhubarb polyoses, has definite molecular weight through collection; Can reach combine with the common pharmaceutical carrier pharmaceutical composition of the rhubarb polyoses produced of collected rhubarb polyoses and be used to treat diabetes, and can be used for antitumor, defying age, enhancing immunity, be applied to clinical.Adopt technical scheme of the present invention simultaneously, it is little that the rhubarb polyoses with definite molecular weight that makes has side effect, the tangible advantage of therapeutic effect.And the rhubarb polyoses pharmaceutical composition of processing; No matter be that injectable powder, tablet, capsule, injection, drop pill, dispersant, oral agents or the nasal drop medicine in drug administration process is with respect to original rhubarb polyoses of not confirming molecular weight; When playing same therapeutic effect; The dosage that need take has all reduced many with respect to prior art, have effect easy to use.
The specific embodiment
Following examples are used to explain the present invention, but are not used in restriction the present invention.Below come further to set forth extraction and separation process of the present invention through Test Example.
Embodiment 1
I. supersound extraction: pulverize after with the roots and stems of rhubarb clean dry (1) is 20 purpose coarse grain.(2) with coarse grain with pure water supersound extraction 3 times, promptly soak after 2 hours with supersound extraction 40 minutes filtration with the pure water of 3 times of amounts; Collect filtrating, filtering residue adds the pure water supersound extraction 40 minutes of 2 times of amounts again, filters; Collect filtrating; Filtering residue adds the pure water supersound extraction 50 minutes of 2 times of amounts again, filters, and collects filtrating.The filtrating of 3 supersound extraction is merged.
II. ultra-filtration and separation:
The filtrating that (3) will merge is used Hollow Fiber Ultrafiltration, the above medicinal liquid of 20,000 molecular weight that dams earlier, and the medicinal liquid that will dam is again collected the medicinal liquid between 2-3 ten thousand molecular weight with the method for ultrafiltration.
(4) medicinal liquid more than 30,000 molecular weight is collected the medicinal liquid between 10-12 ten thousand molecular weight with the method for ultrafiltration.
(5) with above-mentioned (3), (4) twice combining medicine, 60 ℃ are evaporated to drug concentrations is 0.8g/ml.
III. purification:
(6) ethanol precipitation: concentrate is added the dehydrated alcohol of 3 times of amounts, stirred 30 minutes, placed 12 hours, (wherein every stirred 30 minutes, stir altogether 3 times) at a distance from 2 hours, filtering residue is got in centrifugal filtration, must the rhubarb polyoses bullion.
(7) isolating protein: the rhubarb polyoses bullion is used dissolved in distilled water, add trichloroacetic acid by the sevag method and remove protein, in order to reduce Protein content, this method 3-4 time repeatedly as far as possible.
(8) precipitate with ethanol decolouring: add dehydrated alcohol in the proteinic solution and make polysaccharide precipitation above-mentioned the removal, filter, will precipitate and use water dissolution; Add dehydrated alcohol deposition, filter, according to said method repeatedly 4-5 time; Make the color clear of polysaccharide; Filter the back at last with 60 ℃ of aeration-dryings, promptly get rhubarb polyoses, purity＞90%.
Embodiment 2
Rhubarb polyoses in the present embodiment is prepared from through following method:
A, with the roots and stems of rhubarb clean dry, be ground into 30 purpose coarse grain; B, with described coarse grain with the pure water of 5 times of its weight soak, supersound extraction 3 times, filter merging filtrate; C, described filtrating is dammed with Hollow Fiber Ultrafiltration, collected molecular weight and be 2～30,000 medicinal liquid; The method collection molecular weight of d, reuse ultrafiltration is 10～120,000 medicinal liquid;
E, be 10～120,000 combining medicine of 2～30,000 medicinal liquid and 3-12 part volume, concentrate the molecular weight of described 2-8 part volume, the medicinal liquid after must concentrating, the concentration of the medicinal liquid after concentrating reaches 0.5g/ml; F, the medicinal liquid after described concentrate use ethanol precipitation, filtering residue is removed in filtration, obtains the rhubarb polyoses bullion; G, with the described rhubarb polyoses bullion of dissolved in distilled water, and adopt sevag method or trichloroacetic acid method to remove protein wherein, obtain rhubarb polyoses dissolving crude product liquid; H, in described rhubarb polyoses dissolving crude product liquid, add dehydrated alcohol, deposition is filtered; Remove filtering residue, become yellowishly up to the color of described rhubarb polyoses dissolving crude product liquid, no longer change; With the aeration-drying of described rhubarb polyoses dissolving crude product liquid, obtain rhubarb polyoses again.
Following examples are used to set forth preparation of drug combination of the present invention, but do not limit the present invention in any way.
Embodiment 3
The lyophilized injectable powder of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 25g, mannitol 100g, water for injection 1000ml,
Method for preparing:
Embodiment 1 product, mannitol are accurately claimed to decide by prescription, with the water for injection dissolving fully, add 0.1% pin and use activated carbon; Stir (under 60 ℃ of situation, stirring 10 minutes), adding hydrochloric acid or sodium hydroxide adjusting PH is 3.0; Decarburization is filtered, and the filter membrane with 0.4 μ m and 0.22 μ m respectively filters once then, gets final product packing; The false add plug advances the freeze drying box drying.
Embodiment 4
The lyophilized injectable powder of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 500g, mannitol 1g, water for injection 1000ml
Method for preparing:
Embodiment 1 product, mannitol are accurately claimed to decide by prescription, with the water for injection dissolving fully, add 3% pin and use activated carbon; Stir (under 20 ℃ of situation, stirring 90 minutes), adding hydrochloric acid or sodium hydroxide adjusting PH is 7.0; Decarburization is filtered, and the filter membrane with 0.4 μ m and 0.22 μ m respectively filters once then, gets final product packing; The false add plug advances the freeze drying box drying.
Embodiment 5
The lyophilized injectable powder of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 1g, mannitol 500g, water for injection 1000ml,
Method for preparing:
Product, the mannitol of embodiment 1 or 2 are accurately claimed to decide by prescription, with the water for injection dissolving fully, add 0.1% pin and use activated carbon; Stir (under 30 ℃ of situation, stirring 60 minutes), adding hydrochloric acid or sodium hydroxide adjusting PH is 6.0; Decarburization is filtered, and the filter membrane with 0.4 μ m and 0.22 μ m respectively filters once then, gets final product packing; The false add plug advances the freeze drying box drying.
Embodiment 6
The injection liquor (50-500ml) of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 25g, tween 80 1g, sodium chloride (9g/1000ml) 9g, water for injection adds to 1000ml
Method for preparing:
Principal agent is dissolved in the proper amount of water for injection, heated and stirred, 40 ℃ of insulations added tween 80, sodium chloride after 60 minutes; Stirred 10 minutes, and regulated pH value, add 0.05% activated carbon between the 5.5-6.5; Stirred 30 minutes, decarburization is filtered, and adds the injection water and puts in place; With 0.22 μ m and 0.4 μ m filtering with microporous membrane, get final product fill, sterilization.
Embodiment 7
The injection liquor (50-500ml) of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 250g, tween 80 5g, sodium chloride (9g/1000ml) 4.5g, water for injection adds to 500ml
Method for preparing:
Principal agent is dissolved in the proper amount of water for injection, heated and stirred, 70 ℃ of insulations added tween 80, sodium chloride after 20 minutes; Stirred 50 minutes, and regulated pH value, add 5% activated carbon between the 3-4; Stirred 10 minutes, decarburization is filtered, and adds the injection water and puts in place; With 0.22 μ m and 0.4 μ m filtering with microporous membrane, get final product fill, sterilization.
Embodiment 8
The rhubarb polyoses dispersible tablet
Its key component is by weight:
Embodiment 1 or 2 product 100g, microcrystalline Cellulose 100g, pregelatinized Starch 10g, silica 1 5g,
Aspartame 5g, orange flavor 4g, carboxymethyl starch sodium 10g, magnesium stearate 2g, 2% polyvinylpyrrolidone K30 (ethanol: solution (binding agent) 2g water=2: 8)
Method for preparing:
With product, microcrystalline Cellulose, pregelatinized Starch, the silicon dioxide mix homogeneously of embodiment 1 or 2, cross 60 mesh sieves, mix; (ethanol: water=2: 8) solution is processed soft material, granulates with 30 orders, and aeration-drying is 3 hours under 60 ℃ of conditions to add 20% polyvinylpyrrolidone K30; With 20 order granulate; Add aspartame, orange flavor, carboxymethyl starch sodium, magnesium stearate etc. and always mix, tabletting promptly gets.
Embodiment 9
The rhubarb polyoses dispersible tablet
Its key component is by weight:
Embodiment 1 or 2 product 250g, microcrystalline Cellulose 50g, pregelatinized Starch 50g; Silicon dioxide 20g aspartame 10g, orange flavor 7g, carboxymethyl starch sodium 25g; Magnesium stearate 2g, 2% polyvinylpyrrolidone K30 (ethanol: solution (binding agent) 10g water=2: 8)
Method for preparing:
With product, microcrystalline Cellulose, pregelatinized Starch, the silicon dioxide mix homogeneously of embodiment 1 or 2, cross 200 mesh sieves, mix; (ethanol: water=2: 8) solution is processed soft material, granulates with 50 orders, and aeration-drying is 9 hours under 50 ℃ of conditions to add 1% polyvinylpyrrolidone K30; With 50 order granulate; Add aspartame, orange flavor, carboxymethyl starch sodium, magnesium stearate etc. and always mix, tabletting promptly gets.
Embodiment 10
The rhubarb polyoses dispersible tablet
Its key component is by weight:
Embodiment 1 product 25g
Microcrystalline Cellulose 250g
Pregelatinized Starch 20g
Silicon dioxide 5g
Aspartame 1g
Orange flavor 1g
Carboxymethyl starch sodium 5g
Magnesium stearate 3g
2% polyvinylpyrrolidone K30 (ethanol: solution (binding agent) 20g water=2: 8)
Method for preparing:
With embodiment 1 product, microcrystalline Cellulose, pregelatinized Starch, silicon dioxide mix homogeneously, cross 100 mesh sieves, mix; (ethanol: water=2: 8) solution is processed soft material, granulates with 35 orders, and aeration-drying is 5 hours under 55 ℃ of conditions to add 2% polyvinylpyrrolidone K30; With 30 order granulate; Add aspartame, orange flavor, carboxymethyl starch sodium, magnesium stearate etc. and always mix, tabletting promptly gets.
Embodiment 11
The rhubarb polyoses oral cavity disintegration tablet
Its key component is by weight:
Embodiment 1 or 2 product 25g
Microcrystalline Cellulose 250g
The crosslinked carboxylic sodium carboxymethylcellulose pyce 30g of man
Silicon dioxide 20g
Mannitol 50g
Menthol 5g
Aspartame 10g
Essence 1g
Magnesium stearate 5g
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, cross 20,30 mesh sieves each once, get final product tabletting (direct pressure closing).
Embodiment 12
The oral cavity disintegration tablet of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 100g
Microcrystalline Cellulose 80g
The crosslinked carboxylic sodium carboxymethylcellulose pyce 15g of man
Silica 1 0g
Mannitol 100g
Menthol 0.5g
Aspartame 5g
Essence 5g
Magnesium stearate 2.5g
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, cross 50,60 mesh sieves each once, get final product tabletting (direct pressure closing).
Embodiment 13
The rhubarb polyoses oral cavity disintegration tablet
Its key component is by weight:
Embodiment 1 or 2 product 250g
Microcrystalline Cellulose 50g
The crosslinked carboxylic sodium carboxymethylcellulose pyce 50g of man
Silicon dioxide 5g
Mannitol 80g
Menthol 3g
Aspartame 8g
Essence 6g
Magnesium stearate 1g
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, cross 40,50 mesh sieves each once, get final product tabletting (direct pressure closing).
Embodiment 14
The rhubarb polyoses controlled release tablet
Its key component is by weight:
Embodiment 1 or 2 product 100g
Lactose 80g
Hypromellose K
100M50g
Polyvinylpyrrolidone 5g
Magnesium stearate 2g
Method for preparing:
Rhubarb polyoses, lactose, high viscosity hypromellose are crossed 80 mesh sieves, mix homogeneously, (ethanol: water=1: 1) solution is processed soft material to add 10% polyvinylpyrrolidone; Granulate with 20 orders, aeration-drying is 5 hours under 60 ℃ of conditions, with 20 order granulate; Add that magnesium stearate is always mixed, tabletting, promptly get.
Embodiment 15
The rhubarb polyoses effervescent tablet
Its key component is by weight:
Embodiment 1 or 2 product 250g
Anhydrous citric acid 1000g
Sodium bicarbonate 500g
Lactis Anhydrous 100g
Aspartame 30g
Polyethylene glycol 6000 25g
Crospolyvinylpyrrolidone 5g
Dehydrated alcohol 100ml
Essence 5g
Method for preparing:
1) crospolyvinylpyrrolidone, dehydrated alcohol are made into 5% ethanol solution, subsequent use.
2) embodiment 1 product, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous are fed intake by the accurate title of prescription surely, with 60 ℃ of aeration-dryings, 12 hours subsequent use.
3) with dried embodiment 1 product, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous mix homogeneously, add above-mentioned stock solution and process soft material, granulate with 10 orders, 60 ℃ of aeration-dryings are with 10 order granulate.
4) add aspartame, polyethylene glycol 6000, essence mix homogeneously behind the granulate after, get final product tabletting.
Embodiment 16
The rhubarb polyoses effervescent tablet
Its key component is by weight:
Embodiment 1 or 2 product 100g
Anhydrous citric acid 750g
Sodium bicarbonate 750g
Lactis Anhydrous 50g
Aspartame 20g
Polyethylene glycol 6000 10g
Crospolyvinylpyrrolidone 1.5g
Dehydrated alcohol 50ml
Essence 2g
Method for preparing:
1) crospolyvinylpyrrolidone, dehydrated alcohol are made into 3% ethanol solution, subsequent use.
2) embodiment 1 product, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous are fed intake by the accurate title of prescription surely, with 30 ℃ of aeration-dryings, 24 hours subsequent use.
3) with dried embodiment 1 product, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous mix homogeneously, add above-mentioned stock solution and process soft material, granulate with 30 orders, 60 ℃ of aeration-dryings are with 30 order granulate.
4) add aspartame, polyethylene glycol 6000, essence mix homogeneously behind the granulate after, get final product tabletting.
Embodiment 17
The general thin coated tablet of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 100g
Starch 80g
Microcrystalline Cellulose 25g
Hydroxypropyl emthylcellulose 5g
Silicon dioxide 2g
Carboxymethyl starch sodium 5g
Magnesium stearate 2g
Method for preparing:
1) accurately claims fully mix homogeneously of fixed back with product, starch, the microcrystalline Cellulose of embodiment 1 or 2, process soft material with 2% hydroxypropyl emthylcellulose aqueous solution, with 20 mesh sieve system granules.
2) granule is with 60 ℃ of aeration-dryings, and moisture Control is 5%, with 10 mesh sieve granulate.
3) add silicon dioxide, carboxymethyl starch sodium, magnesium stearate mix homogeneously after, get final product tabletting.
4) preparation of coating solution (by weight)
Hydroxypropyl emthylcellulose 1g
Polyethylene glycol 6000 3g
Oleum Ricini 0.5g
Diethyl phthalate 3g
95% ethanol 1g
It is fixed that above supplementary material is accurately claimed, stirs, and until dissolving fully, it is subsequent use to cross 50 mesh sieves.
5) pack film-coat by conventional method.
Embodiment 18
The hard capsule of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 50g
Carboxymethyl starch sodium 80g
Silicon dioxide 5g
Magnesium stearate 2g
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, last fully-automatic capsule filling machine fill hard capsule.
Embodiment 19
The hard capsule of rhubarb polyoses
Its key component is by weight:
Embodiment 1 or 2 product 100g
Carboxymethyl starch sodium 50g
Silica 1 0g
Magnesium stearate 1g
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, last fully-automatic capsule filling machine fill hard capsule.
Embodiment 20
The granule of rhubarb polyoses
Rhubarb polyoses 100g
Sucrose 250g
Microcrystalline Cellulose 50g
Hypromellose 3g
Silicon dioxide 5g
Method for preparing:
It is fixed that main and auxiliary raw material is accurately claimed by prescription, adds 3-30% hydroxypropyl emthylcellulose aqueous solution, processes soft material; Granulate with the 10-30 mesh sieve, with 30-70 ℃ of aeration-drying, with 80 orders and the screening of 10 mesh sieves; After removing the granule of < 80 with>10 mesh sieves, pack promptly with particles packing machine and to get.
Embodiment 21
The granule of rhubarb polyoses (Sugarless type)
Its key component is by weight:
Embodiment 1 or 2 product 50g
Xylitol 100g
Mannitol 50g
Microcrystalline Cellulose 50g
Hypromellose 5g
Silica 1 0g
Method for preparing:
It is fixed that main and auxiliary raw material is accurately claimed by prescription, adds 3% hydroxypropyl emthylcellulose aqueous solution, processes soft material; Granulate with 30 mesh sieves, with 30 ℃ of aeration-dryings, with 80 orders and the screening of 10 mesh sieves; After removing the granule of < 80 with>10 mesh sieves, pack promptly with particles packing machine and to get.
Embodiment 22
Modernization Theory and method summary go out rhubarb polyoses Pharmaceutical composition prescription and preparation method thereof, and be specific as follows:
The rhubarb polyoses lyophilized injectable powder
Its key component is by weight:
Rhubarb polyoses 1-500 part
Mannitol 1-500 part
Water for injection 10-1000ml
Method for preparing:
Rhubarb polyoses, mannitol are accurately claimed to decide by prescription, with the water for injection dissolving fully, add the 0.01-3% pin and use activated carbon; Stir (under 20-60 ℃ of situation, stirring 10-100 minute), adding hydrochloric acid or sodium hydroxide adjusting PH is between the 2-8.0; Decarburization is filtered, and the filter membrane with 0.1-0.9 μ m filters 1-3 time then, gets final product packing; The false add plug advances the freeze drying box drying.
Embodiment 23
Rhubarb polyoses injection (50-500ml)
Its key component is by weight:
Rhubarb polyoses 1-500 part
Tween 80 0.1-10 part
Sodium chloride (9g/1000ml) 0.1-9 part
Water for injection adds to 10-1000ml
Method for preparing:
Principal agent is dissolved in the proper amount of water for injection, heated and stirred, 40-70 ℃ of insulation added tween 80, sodium chloride after 20-60 minute; Stirred 10-50 minute, and regulated pH value, add the 0.05-6% activated carbon between the 3-8; Stirred 10-50 minute, decarburization is filtered, and adds the injection water and puts in place; With 0.1-6 μ filtering with microporous membrane, get final product fill, sterilization.
Embodiment 24
The rhubarb polyoses dispersible tablet
Its key component is by weight:
Rhubarb polyoses 25-250 part
Microcrystalline Cellulose 25-250 part
Pregelatinized Starch 10-50 part
Silicon dioxide 5-20 part
Aspartame 1-10 part
Essence 1-7 part
Carboxymethyl starch sodium 2-25 part
Magnesium stearate 0.5-5 part
2% polyvinylpyrrolidone K30 (ethanol: solution (binding agent) 2-50 part water=2: 8)
Method for preparing:
With rhubarb polyoses, microcrystalline Cellulose, pregelatinized Starch, silicon dioxide mix homogeneously, cross the 30-200 mesh sieve, mix; (alcohol: water=2.8) solution is processed soft material to add 1-20% polyvinylpyrrolidone K30; Granulate with the 20-50 order, under 50-60 ℃ of condition aeration-drying 1-9 hour, with 10-50 order granulate; Add total mixed, tabletting such as aspartame, essence, carboxymethyl starch sodium, magnesium stearate, promptly get.
Embodiment 25
The rhubarb polyoses oral cavity disintegration tablet
Its key component is by weight:
Rhubarb polyoses 25-250 part
Microcrystalline Cellulose 25-250 part
The crosslinked carboxylic sodium carboxymethylcellulose pyce 10-50 of man part
Silicon dioxide 5-30 part
Mannitol 10-200 part
Menthol 0.5-5 part
Aspartame 1-15 part
Essence 1-15 part
Magnesium stearate 0.5-5 part
Method for preparing:
After accurately title was decided by prescription with main auxiliary material, fully mix homogeneously was crossed 20-100 mesh sieve twice, gets final product tabletting (direct pressure closing).
Embodiment 26
The rhubarb polyoses controlled release tablet
Its key component is by weight:
Rhubarb polyoses 25-250 part
Lactose 10-150 part
High viscosity hypromellose 10-200 part
Polyvinylpyrrolidone 10-50 part
Magnesium stearate 0.5-5 part
Method for preparing:
Rhubarb polyoses, lactose, high viscosity hypromellose are crossed the 60-100 mesh sieve; Mix homogeneously, (ethanol: water=1: 1) solution is processed soft material, granulates with the 10-50 order to add the 5-20% polyvinylpyrrolidone; Under 30-60 ℃ of condition aeration-drying 1-10 hour; With 10-50 order granulate, add that magnesium stearate is always mixed, tabletting, promptly get.
Embodiment 27
The rhubarb polyoses effervescent tablet
Its key component is by weight:
Rhubarb polyoses 25-250 part
Anhydrous citric acid 50-1000 part
Sodium bicarbonate 50-1000 part
Lactis Anhydrous 20-200 part
Aspartame 5-100 part
Polyethylene glycol 6000 10-100 part
Crospolyvinylpyrrolidone 1-50 part
Dehydrated alcohol 20-200 part
Essence 1-5 part
Method for preparing:
1) crospolyvinylpyrrolidone, dehydrated alcohol are made into the 1-15% ethanol solution, subsequent use.
2) rhubarb polyoses, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous are fed intake by the accurate title of prescription surely, with 30-60 ℃ of aeration-drying, subsequent use.
3) with dried rhubarb polyoses, anhydrous citric acid, sodium bicarbonate, Lactis Anhydrous mix homogeneously, add above-mentioned stock solution and process soft material, to granulate with the 10-30 order, 30-60 ℃ of aeration-drying is with 10-20 order granulate.
4) add aspartame, polyethylene glycol 6000 mix homogeneously behind the granulate after, get final product tabletting.
Embodiment 28
The general thin coated tablet of rhubarb polyoses
Its key component is by weight:
Rhubarb polyoses 25-250 part
Starch 10-200 part
Microcrystalline Cellulose 5-60 part
Hydroxypropyl emthylcellulose 1-10 part
Silicon dioxide 2-20 part
Carboxymethyl starch sodium 1-20 part
Magnesium stearate 1-5 part
Method for preparing:
1) accurately claims fully mix homogeneously of fixed back with rhubarb polyoses, starch, microcrystalline Cellulose, process soft material with 1-20% hydroxypropyl emthylcellulose aqueous solution, with 10-30 mesh sieve system granule.
2) granule is with 30-60 ℃ of aeration-drying, and moisture Control is between 1.5%-10%, with 10-30 mesh sieve granulate.
3) add silicon dioxide, carboxymethyl starch sodium, magnesium stearate mix homogeneously after, get final product tabletting.
4) preparation of coating solution (by weight)
Hydroxypropyl emthylcellulose 1-5 part
Polyethylene glycol 6000 0.5-3 part
Oleum Ricini 0.5-3 part
Diethyl phthalate 0.5-3 part
95% ethanol 1-10 part
It is fixed that above supplementary material is accurately claimed, stirs, and until dissolving fully, it is subsequent use to cross the 50-150 mesh sieve.
5) pack film-coat by conventional method.
Embodiment 29
The hard capsule of rhubarb polyoses
Its key component is by weight:
Rhubarb polyoses 25-250 part
Carboxymethyl starch sodium 10-200 part
Silicon dioxide 2-30 part
Magnesium stearate 0.5-5 part
Method for preparing:
With main auxiliary material by prescription accurately claim fixed after, abundant mix homogeneously, last fully-automatic capsule filling machine fill hard capsule.
Embodiment 30
The granule of rhubarb polyoses
Rhubarb polyoses 25-100 part
Sucrose 20-500 part
Microcrystalline Cellulose 20-100 part
Hypromellose 1-5 part
Silica 1-20 part
Method for preparing:
It is fixed that main and auxiliary raw material is accurately claimed by prescription, adds 3-30% hydroxypropyl emthylcellulose aqueous solution, processes soft material; Granulate with the 10-30 mesh sieve, with 30-70 ℃ of aeration-drying, with 80 orders and the screening of 10 mesh sieves; After removing the granule of < 80 with>10 mesh sieves, pack promptly with particles packing machine and to get.
Embodiment 31
The granule of rhubarb polyoses (Sugarless type)
Its key component is by weight:
Rhubarb polyoses 25-250 part
Xylitol 20-250 part
Mannitol 20-200 part
Microcrystalline Cellulose 20-100 part
Hypromellose 1-5 part
Silica 1-20 part
Method for preparing:
It is fixed that main and auxiliary raw material is accurately claimed by prescription, adds 3-30% hydroxypropyl emthylcellulose aqueous solution, processes soft material; Granulate with the 10-30 mesh sieve, with 30-70 ℃ of aeration-drying, with 80 orders and the screening of 10 mesh sieves; After removing the granule of < 80 with>10 mesh sieves, pack promptly with particles packing machine and to get.
Following embodiment will further set forth the effect of rhubarb polyoses pharmaceutical composition aspect blood sugar lowering
Embodiment 32
Influence to normal mouse
The preparation method of rhubarb polyoses is got 50 of mices with reference to embodiment 1, is divided into the high, medium and low dose groups (0.4 of rhubarb polyoses at random; 0.2,0.1g/kg) glyburide 2.5mg/kg positive controls, normal control group; Every group 10, each group is pressed 0.2mL/10g gastric infusion every day or normal saline, continuous 5 days; Fasting 12h before the last administration, 1h mouse orbit measuring blood sugar of blood extracting value after the administration.The result sees the following form: (n=10)
| Group | Dosage/gkg -1 | Blood glucose value/mmolL -1 |
| The normal control group | 5.6±0.4 | |
| The rhubarb polyoses high dose group | 0.4 | 4.7±0.7 |
| Dose groups in the rhubarb polyoses | 0.2 | 4.9±0.6 |
| The rhubarb polyoses low dose group | 0.1 | 4.7±0.7 |
| The glyburide group | 0.0025 | 5.2±0.4 |
With the normal control group relatively, P＜0.01 this shows that the rhubarb polyoses among the present invention also has tangible reduction effect to the blood glucose of normal mouse.
Following embodiment rhubarb polyoses compositions can improve the content of serum insulin except having hypoglycemic effect.The effect that also has antitumor, defying age, enhancing immunity.
Embodiment 33
Following examples are set forth the inhibitory action of rhubarb polyoses for tumor cell.
Rhubarb polyoses is taken from the rhubarb polyoses RRP in the embodiment of the invention 2, S
180The tumor strain is provided by the Beijing Inst of Tumor Prevention and Treatment, selects inoculation S
180The mice in one week of ascitic type tumor strain is got ascites by conventional method, and the adjusting cell concentration is 1*10
10/ L is used for transplanting injection, and every Mus is injected 0.2ml; The YA6-1 cell, available from Beijing's prevention and cure of occupational disease institute, kunming mice, body weight 18-22g is provided by Mountain Western Medicine S University's animal center, and 40 of ♂ mices are divided 4 groups of ig administrations at random, continuous 10d, d6 inoculates S
180The tumor strain is subcutaneous in right axil, and d15 after the administration (promptly inoculate tumor strain after d10) puts to death mice, gets tumor respectively and weighs, and calculates tumour inhibiting rate.Rhubarb polyoses is to the tumor-inhibiting action of mice, and the result sees table, the inhibitory rate to 56.2% of RRP:
| Medicine | Dosage (mg/kg) | Tumor heavy (g) | Tumour inhibiting rate (%) |
| RRP | 100 | 0.78±0.66 | 56.2 A |
| RRP | 400 | 1.20±0.67 | 32.5 B |
The P of A＜0.05, the P of B＜0.01
Embodiment 34
Following examples will be explained the influence of rhubarb polyoses to immune organ, see the following form, and calculate spleen index and thymus index, compare with cyclophosphamide
| Medicine | Dosage (mg/kg) | Spleen index | Thymus index |
| Contrast | 0 | 5.4±1.8 | 1.6±0.9 |
| Cyclophosphamide | 20 | 4.4±1.0 B | 1.3±0.5 B |
| Rhubarb polyoses+cyclophosphamide | 20+100 | 5.3±1.6 | 1.6±0.4 |
| Rhubarb polyoses | 50 | 5.6±2.0 | 1.9±1.5 |
| Rhubarb polyoses | 100 | 5.6±1.0 | 1.9±0.9 |
Visible from last table; The body weight of cyclophosphamide group is negative growth; Spleen index and thymus index all significantly descend, other three experimental group body weight and organ index and matched group comparison there are no significant difference, explanation; Cyclophosphamide has inhibitory action to immunologic function, and rhubarb polyoses 100mg/kg has protective effect to the immunity infringement that cyclophosphamide causes.
Embodiment 35
Following examples will be explained the experimentation of rhubarb polyoses antioxidation; Get the rhubarb polyoses that embodiment 1 or embodiment 2 obtain; Detect its influence to aging model mouse red blood cell SOD vigor, taking heparin anticoagulant mice whole blood prepares the erythrocyte sod extract, adopts xanthine oxidase on 721 spectrophotometers, to measure; Test kit and experimental technique build up bio-engineering research by Nanjing and provide, and formula calculates the vigor of SOD to specifications.
To the influence of aging model mouse red blood cell catalase (CAT) vigor, isolate erythrocyte after anticoagulant heparin Mus blood is centrifugal, make into 1: 100 normal saline hemolysate, press literature method mensuration and calculate the peroxidase vigor, the result sees table.
Mus blood 10 μ l are got in the influence of aging model mice whole blood glutathion peroxidase (GSH-Px) vigor; Be diluted to 1ml with distilled water, press literature method after enzymatic reaction and chromogenic reaction, the 422nm place surveys a pipe OD value; Establish simultaneously the non-enzyme reaction control tube in addition; Investigate the influence of non-enzyme system to GSH, calculate the GSH-Px vigor, the result sees table.
The result shows; Compare with the blank group; Model group zoology erythrocyte sod, CAT vigor and GSH-Px vigor obviously reduce (P＜0.01), show that modeling is successful, compare with model group; Radix Et Rhizoma Rhei bull can obviously raise aging model mice erythrocyte SOD, CAT vigor and blood GSH-Px activity level (P＜0.01) show that rhubarb polyoses can be through improving aging relevant enzyme vigor performance function in delaying senility.
Rhubarb polyoses is to the influence of aging model mice blood SOD, CAT and GSH-Px vigor
| Group | Dosage (mg/kg) | The SOD vigor (μ/ml) | The CAT vigor (second -1) (K value) | GSH-Px(μ/ml/min) |
| The blank group | 466.4±73.6 B | 9.118±0.027 B | 7.94±1.98 B | |
| Model group | 280.3±58.9 | 6.661±0.771 | 3.47±0.52 B | |
| The lentinan low dose group | 100 | 466.9±69.7 B | 10.07±1.809 B | 6.76±1.64 B |
| Dose groups in the lentinan | 200 | 472.9±40.5 B | 9.081±1.477 B | 7.62±1.09 B |
| Senior two groups of lentinan | 400 | 481.4±91.6 B | 8.654±0.956 B | 6.70±1.81 B |
Compare with model group; The rhubarb polyoses of basic, normal, high three dosage and lentinan all can obviously reduce the lipid peroxide level of aging model mice plasma, LH and brain homogenate; (P＜0.01); Show that rhubarb polyoses helps to remove the aging metabolite of body, rhubarb polyoses can be through improving aging relevant enzyme vigor, eliminates or reduce aging metabolite and reach anti-aging effects.
Claims (5)
1. rhubarb polyoses, it is characterized in that: described rhubarb polyoses adopts following method to be prepared from:
I. supersound extraction: pulverize after with the roots and stems of rhubarb clean dry (1) is 20 purpose coarse grain.(2) with coarse grain with pure water supersound extraction 3 times, promptly soak after 2 hours with supersound extraction 40 minutes filtration with the pure water of 3 times of amounts; Collect filtrating, filtering residue adds the pure water supersound extraction 40 minutes of 2 times of amounts again, filters; Collect filtrating; Filtering residue adds the pure water supersound extraction 50 minutes of 2 times of amounts again, filters, and collects filtrating; The filtrating of 3 supersound extraction is merged;
II. ultra-filtration and separation:
The filtrating that (3) will merge is used Hollow Fiber Ultrafiltration, the above medicinal liquid of 20,000 molecular weight that dams earlier, and the medicinal liquid that will dam is again collected the medicinal liquid between 2-3 ten thousand molecular weight with the method for ultrafiltration;
(4) medicinal liquid more than 30,000 molecular weight is collected the medicinal liquid between 10-12 ten thousand molecular weight with the method for ultrafiltration;
(5) with above-mentioned (3), (4) twice combining medicine, 60 ℃ are evaporated to drug concentrations is 0.8g/ml;
III. purification:
(6) ethanol precipitation: concentrate is added the dehydrated alcohol of 3 times of amounts, stirred 30 minutes, placed 12 hours, wherein whenever stirred 30 minutes at a distance from 2 hours, stir altogether 3 times, filtering residue is got in centrifugal filtration, must the rhubarb polyoses bullion;
(7) isolating protein: the rhubarb polyoses bullion is used dissolved in distilled water, add trichloroacetic acid by the sevag method and remove protein, in order to reduce Protein content, this method 3-4 time repeatedly as far as possible;
(8) precipitate with ethanol decolouring: add dehydrated alcohol in the proteinic solution and make polysaccharide precipitation above-mentioned the removal, filter, will precipitate and use water dissolution; Add dehydrated alcohol deposition, filter, according to said method repeatedly 4-5 time; Make the color clear of polysaccharide; Filter the back at last with 60 ℃ of aeration-dryings, promptly get rhubarb polyoses, purity＞90%.
2. a rhubarb polyoses pharmaceutical composition that contains the said rhubarb polyoses of claim 1 is characterized in that it comprises described rhubarb polyoses and pharmaceutically acceptable carrier.
3. rhubarb polyoses pharmaceutical composition according to claim 2; It is characterized in that described rhubarb polyoses pharmaceutical composition comprises: the carrier of the rhubarb polyoses of 1 weight portion and 0.5-200 weight portion, described carrier are one or more the combination in mannitol, sorbitol, dextran, glucose, lactose, sucrose, sodium chloride, citric acid, sodium bicarbonate, hypromellose, low-substituted hydroxypropyl cellulose, crospolyvinylpyrrolidone, polyvinylpyrrolidone, microcrystalline Cellulose, poloxamer, tween 80, carboxymethyl starch sodium, pregelatinized Starch, starch, silicon dioxide, xylitol, Macrogol 4000-6000, beta-schardinger dextrin-, cross-linking sodium carboxymethyl cellulose, carboxymethylcellulose calcium or the acrylic resin.
4. rhubarb polyoses pharmaceutical composition according to claim 2; It is characterized in that described pharmaceutical composition is the pharmaceutical composition of gastrointestinal tract, injection or respiratory tract administration, and described pharmaceutical composition has following dosage form: injectable powder, capsule, injection, drop pill, tablet, nasal drop.
5. be used to prepare the application on treatment diabetes or the antiaging agent according to each described rhubarb polyoses pharmaceutical composition of claim 2-4.
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Non-Patent Citations (5)
| Title |
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| 刘莉等.大黄多糖提取工艺对糖含量和糖醛酸含量的影响.<<中国药学杂志>>.2003,第38卷(第10期),748-750. * |
| 姚广涛等.掌叶大黄多糖抗氧化作用的实验研究.<<中医药学刊>>.2004,第22卷(第7期),295、1311. * |
| 张思巨.大黄多糖的研究.<<中国中药杂志>>.1993,第18卷(第11期),679-681. * |
| 蒋滢等.大黄多糖分离纯化及其功能的研究.<<药物生物技术>>.2002,第9卷(第6期),330-333. * |
| 金槿实等.掌叶大黄多糖对肿瘤DNA合成抑制作用的研究.<<吉林中医药>>.1999,(第4期),58-59. * |
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