CN1013544B - 判断树龄的方法 - Google Patents
判断树龄的方法Info
- Publication number
- CN1013544B CN1013544B CN 90103136 CN90103136A CN1013544B CN 1013544 B CN1013544 B CN 1013544B CN 90103136 CN90103136 CN 90103136 CN 90103136 A CN90103136 A CN 90103136A CN 1013544 B CN1013544 B CN 1013544B
- Authority
- CN
- China
- Prior art keywords
- bark
- tree
- age
- judgement
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
本发明公开了一种判断树龄的方法,其特征在于取一小块树皮,用水或甘油加水(以1∶1或2∶1比例混溶)浸泡树皮1~2周或者在沸水中煮10~30分钟软化树皮,然后切制成10~30微米厚的薄片,用I.IK溶液染色后,在显微镜下观察韧皮纤维束构成的环带层数即树皮年轮,可以达到鉴别树龄的目的。其特点在于简便,易行,无害树体。对于外皮不易剥落,树皮年轮明显的树种最为适用。
Description
本发明涉及一种用树皮鉴定树龄的新方法,特别是对某些“树皮年龄”明显、外皮不易剥落的树种最宜适用。
树木年龄的判断是林业生产及生药研究中经常遇到的问题,在林业方面可根据树龄进行有计划的采伐、在生药质量控制方面,可根据树龄研究植物体中有效成分含量的变化规律,指导药材的生产与质量检测。以往国内外判断树龄的方法多以树干基部的年轮为依据。通常是将树木横断或用生长锥,从树干钻取试样材,计数木质部年轮。以上方法既伤害树木又费时费力。放射性同位素测定树龄以百年为计量单位,误差至少数十年,且由于条件限制不便推广。有些树种的枝龄可根据叶痕形成的“年节”判断,但仅适用于幼枝。总之,迄今国内外尚无迅速、简便且无碍树木生长的确定树龄的方法。
本发明的目的是要提供一种判断树龄的新方法,它既不伤害树木又判断准确且简便易行。
本发明是通过在显微镜下观察“树皮年轮”来判断树龄的。其具体做法是,取树皮一小块,视树皮质地情况用水或甘油和水等进行软化,软化后视情况采用手工切片、滑走切片或冰冻切片法制片,切片可视情况制成10~30微米厚,制好的切片用I·IK溶液(碘0.5g与碘化钾1.0g加水20ml溶解即得)染色后备用。前处理完成后,将切片于显微镜下观察“树皮年轮”,即由韧皮纤维束构成环带的层数。由此达到鉴定树龄的目的。
本法已经过百余种植物反复实验得以证实。本发明方法特别适用于木兰科(木莲属、含笑属、木兰属、八角属)、秦皮、丁香、核桃、合欢、银合欢、黄柏、花椒、栋树、杨树、臭椿、栾树等树种。
根据本发明,树皮软化后手工切制成10~30微米厚的薄片,用I·IK溶液(碘0.5g与碘化钾1.0g加水20ml溶解即得)染色10~30分钟后,在显微镜下观察韧皮纤维束构成的环带层数即“树皮年轮”(树皮年轮等于树龄)。
根据本发明,树皮最好取材于树干基部背阴面处;树皮的大小以0.5×1.0厘米~1.0×2.0厘米为宜;软化树皮可在常温下用水浸泡树皮1~2周,或在沸水中煮10~30分钟。也可用甘油与水以1∶1或2∶1比例混溶后浸泡树皮1~2周;制片也可用滑走切片或冰冻切片法。
研究表明运用本发明能非常清晰、非常准确地观察“树皮年轮”,从而准确地判断树龄。此外,本发明不但判断树龄精确,而且无害树体、操作也十分简便。更可观的是,本发明为树龄的判断开创了一个新的途径,具有很高的学术价值和经济价值。
以下实例是对本发明的进一步说明。
实施例1:
取二乔玉兰(Magnolia soulangeana)背阴面树皮一块(0.5×1.0厘米),树皮厚0.9厘米,沸水煮20分钟,手工切片(约20微米厚片)用I·IK溶液染色后于显微镜下观察,可见纤维束环带构成的“树皮年轮”与“木质部年轮”呈对应关系。
实施例2:
取望春玉兰(Magnolia biondii)(该树木产于河南省南召县皇后乡天桥村,树高20多米,胸径1.38米,冠幅800多平方米,每年产辛夷花180多公斤,被誉为“辛夷王”),树干基部背阴面树皮一块(1.0×2.0厘米),皮厚约2.0厘米,甘油∶水=2∶1的溶液中浸泡二周,手工切片后用I·IK溶液染色后,于显微镜下观察,纤维束环带组成的“树皮年轮”清析易辨,经计数该树树龄为88~92年经核实与史料相符。
实施例3:
取栾树(Koelreuteria paniculata)树干基部完整树皮一块(0.5×1.0厘米),用甘油∶水=1∶1溶液浸泡一周后,冰冻切片制片,染色(I.IK)后于显微镜下观察,可见此时树皮内已产生新的木栓形成层,呈斜向交错相连,但分布于落皮层中的纤维束环带无明显变形,与分布于韧皮部中的纤维束环带之和,同木质部的年轮数相对应。
Claims (6)
1、一种判断树龄的方法,其特征在于取一小块树皮,用水或甘油加水软化树皮,然后切制成10~30微米厚的薄片,用I.IK溶液染色后,在显微镜下观察韧皮纤维束构成的环带层数即树皮年龄。
2、如权利要求1所述的判断树龄的方法,其特征在于树皮最好取材于树干基部背阴面处;树皮的大小以0.5×1.0~1.0×2.0厘米为宜。
3、如权利要求1所述的判断树龄的方法,其特征是在常温下用水浸泡1~2周来软化树皮。
4、如权利要求1所述的判断树龄的方法,其特征在于用沸水煮10~30分钟软化树皮。
5、如权利要求1所述的判断树龄的方法,其特征在于用以1∶1或2∶1的比例混溶的甘油与水浸泡软化树皮。
6、如权利要求1所述的判断树龄的方法,其特征在于制片用滑走切片或冰冻切片法。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 90103136 CN1013544B (zh) | 1990-06-27 | 1990-06-27 | 判断树龄的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 90103136 CN1013544B (zh) | 1990-06-27 | 1990-06-27 | 判断树龄的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1046832A CN1046832A (zh) | 1990-11-14 |
CN1013544B true CN1013544B (zh) | 1991-08-21 |
Family
ID=4877918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN 90103136 Expired CN1013544B (zh) | 1990-06-27 | 1990-06-27 | 判断树龄的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN1013544B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103940738B (zh) * | 2013-01-17 | 2016-08-10 | 复旦大学 | 一种快速鉴别人参年龄的方法 |
CN103279636B (zh) * | 2013-04-10 | 2016-03-02 | 北京林业大学 | 一种确定树木年龄的方法 |
CN103994906A (zh) * | 2014-04-30 | 2014-08-20 | 河南科技大学 | 木材横截面逐年轮显微切片的制作方法 |
CN105738183A (zh) * | 2016-02-29 | 2016-07-06 | 南通香佳纺织科技有限公司 | 一种检测沙漠植物梭梭年轮的方法 |
-
1990
- 1990-06-27 CN CN 90103136 patent/CN1013544B/zh not_active Expired
Also Published As
Publication number | Publication date |
---|---|
CN1046832A (zh) | 1990-11-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Priestley | Studies in the physiology of cambial activity. III. The seasonal activity of the cambium | |
Farrar | Longitudinal variation in the thickness of the annual ring | |
Wolter | A new method for marking xylem growth | |
Gindel | Stomatal number and size as related to soil moisture in tree xerophytes in Israel | |
Yigit et al. | Identification of some fiber characteristics in Rosa sp. and Nerium oleander L. wood grown under different ecological conditions | |
Fielding | Trees grown from cuttings compared with trees grown from seed (Pinus radiata D. Don) | |
Hatton | Apple rootstock studies effect of layered stocks upon the vigour and cropping of certain scions | |
CN1013544B (zh) | 判断树龄的方法 | |
Swarbrick | Factors Governing Fruit Bud Formation.: VIII. The Seasonal Elongation Growth of Apple Varieties on Some Vegetative Rootstocks; and its Possible Relation to Fruit Bud Formation. | |
CN105850521A (zh) | 一种马尾松的嫁接方法 | |
Arnold et al. | Raising and managing radiata pine cuttings for production forests | |
FISHER | Induction of reaction wood in Terminalia (Combretaceae): roles of gravity and stress | |
Reukema | Relation of pine forest overstory opening diameter to growth of pine reproduction | |
Pratt | Fomes annosus Butt-Rot of Sitka Spruce: I. Observations on the Development of Butt-Rot in Individual Trees and in Stands | |
Sledge | The Rooting of Woody Cuttings Considered from the Standpoint of Anatomy. | |
Nour-Eldin | Citrus virus disease research in Egypt | |
CN108703070A (zh) | 一种利用组培快速繁殖海州常山的方法 | |
Tingley | Concentration gradients in plant exudates with reference to the mechanism of translocation | |
Safford | Seasonal variation in the growth and nutrient content of yellow-birch replacement roots | |
Mead | Comparative height growth of eastern larch and black spruce in northwestern Ontario | |
Stiell | Stem growth reaction in young red pine to the removal of single branch whorls | |
Julin et al. | The fluted western hemlock of Alaska. II. Stand observations and synthesis | |
Silen | Earlier forecasting of Douglas-fir cone crop using male buds | |
Burdekin | Gale damage to amenity trees | |
LI et al. | Transpiration characteristics and influencing factors of the dominant species of Machilus microcarpa in Maolan Karst forest |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C06 | Publication | ||
PB01 | Publication | ||
C13 | Decision | ||
GR02 | Examined patent application | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C19 | Lapse of patent right due to non-payment of the annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |