CN101351556B - Plants having improved growth characteristics and a method for making the same - Google Patents

Plants having improved growth characteristics and a method for making the same Download PDF

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CN101351556B
CN101351556B CN200680049144XA CN200680049144A CN101351556B CN 101351556 B CN101351556 B CN 101351556B CN 200680049144X A CN200680049144X A CN 200680049144XA CN 200680049144 A CN200680049144 A CN 200680049144A CN 101351556 B CN101351556 B CN 101351556B
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plant
nucleic acid
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CN101351556A (en
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A·I·桑兹莫林纳罗
C·勒佐
Y·海茨费尔德
V·布多夫
L·德维尔德
D·因泽
V·米隆诺弗
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CropDesign NV
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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Abstract

The present invention relates generally to the field of molecular biology and concerns a method for improving plant growth characteristics relative to corresponding wild type plants. More specifically, the present invention concerns a method for improving plant growth characteristics comprising modulating expression in a plant of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or comprising modulating expression in a plant of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (referred to as YEP16); or comprising modulating expression in a plant of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof. The present invention also concerns plants having modulated expression of a nucleic acid encoding a class I homeodomain leucine zipper (HDZip) hox5 polypeptide or a homologue thereof; or having modulated expression of a nucleic acid encoding a nitrate transporter protein (NRT) or a homologue thereof; or having modulated expression of a nucleic acid encoding a polypeptide denoted Yield Enhancing Protein 16 (hereinafter referred to as YEP16); or having modulated expression of a Group I glycogen synthase kinase (Group I shaggy-like kinase) or a homologue thereof, which plants have improved growth characteristics relative to corresponding wild type plants. The invention also provides constructs useful in the methods of the invention.

Description

Has plant of improvement growth characteristics and preparation method thereof
Invention field
Relate generally to biology field of the present invention, and relate to method with respect to corresponding wild-type plant or other control plants improvement plant growth characteristics.More specifically, the present invention relates to improve the method for plant growth characteristics, comprise regulate I class homeodomain leucine zipper in the plant ( HOme oDomainleucine zIpper, HDZip) expression of hox5 polypeptide or its homologue coding nucleic acid; Or comprise the expression of regulating nitrate transport protein (NRT) in the plant or its homologue coding nucleic acid; Or comprise regulate called after volume increase protein 16 in the plant ( YIeld ENhancing PRotein 16, hereinafter referred to as YEP16) polypeptide or the expression of its homologue coding nucleic acid; Or comprise the expression of regulating I type glycogen synthase kinase (I type shaggy sample kinases) in the plant or its homologue coding nucleic acid.The invention still further relates to the plant that I class HDZip hox5 polypeptide with adjusting or its homologue coding nucleic acid are expressed; Or has a plant that the NRT polypeptide of adjusting or its homologue coding nucleic acid are expressed; Or the plant of expressing of the YEP16 coding nucleic acid with adjusting; Or has a plant that the I type shaggy sample kinases of adjusting or its homologue coding nucleic acid are expressed; Described plant has the plant growth characteristics of improvement with respect to corresponding wild-type or other control plants.The present invention also is provided at construct useful in the inventive method.
Background of invention
In view of world population constantly increases and the agricultural area that can till the land dwindles gradually, evoke the research that improves the farm efficiency aspect.The ordinary method of crop and gardening improvement is to utilize breeding technique to identify to have the plant of desired characteristic.Yet such breeding technique has some shortcomings, and namely these technology are normally labor-intensive, and produces the plant that contains the allogeneic heredity component usually, and these hereditary components are not the anticipant character that always produces from mother plant heredity.Molecular biological progress has allowed the germplasm (germplasm) of human reconstruction animal and plant.Genetically engineered plant need separate and operate genetic material (being generally the form of DNA or RNA) and reach subsequently with described genetic material introduced plant.Such technology can provide crop or the plant of economy, agricultural or gardening proterties with multiple improvement.
The proterties that has economic interests especially is productive rate.Productive rate is normally defined the output measured that is worth from crop economy.It can define according to quantity and/or quality.Productive rate directly depends on several factors, for example: the quantity of organ and size, plant structure (for example branch amount), seed production, leaf aging etc.The growth of root, dietetic alimentation and stress tolerance also are the important factors that determines productive rate.Therefore optimize the productive rate that one of above-mentioned factor can help to increase crop.
The seed productive rate is the proterties that is even more important, and reason is that the seed of many plants is most important for human and animal's nutrition.Account for the over half of human total calorie of intake such as the crop of cereal, rice class, wheat, rape and soybean, no matter be the direct consumption by seed itself, the still consumption by the meat products of being raised by the seed of processing.They also are the sources of the used carbohydrate of industrial processes, oils and multiclass metabolite.Seed contains embryo (new bud and the source of root) and endosperm (nutrition source of embryonic development in sprouting and seedling early growth process).The growth of seed relates to many genes, and needs metabolite to be transferred to the seed of growing from root, leaf and stem.Endosperm particularly absorbs the metabolic precursor thereof of carbohydrate, oils and protein, it is synthesized the storage polymer, so that grain is grown up.
Another proterties that has economic interests especially is to have the abiotic stress tolerance of raising.Abiotic stress is the first cause that causes whole world crop loss, and the average yield of most of staple crop plants is descended above 50% (Wang etc., Planta (2003) 218:1-14).Abiotic stress can cause because of arid, salinity, extreme temperature, chemical toxicity and oxidative stress.Improving the ability of plant abiotic stress tolerance will bring great economic interests to global farmer, and will make we can be under unfavourable condition and otherwise raise crop is come in region that can not raise crop.
The ability that increases the plant seed productive rate will have many application in such as fields such as agriculturals, comprise production, arboriculture, gardening and the forest industry of ornamental plant.Increase productive rate and also can produce (be used for the biotechnology production such as materials such as medicine, antibody or vaccines, or be used for the bio-transformation of organic waste) and other this class field for the algae of using at bio-reactor.
Technical background
Homeodomain leucine zipper (HDZip) albumen
Homeodomain leucine zipper (HDZip) albumen constitutes transcription factor family, it is characterized in that existing DNA binding domains (HD) and contiguous leucine zipper (Zip) motif.Homeodomain generally includes 60 conservative amino-acid residues, forms the spiral 1-ring-spiral 2-corner-spiral 3 of being combined with DNA.This DNA binding site is generally false palindrome.Leucine zipper is adjacent to the C-end of homeodomain, comprises several seven peptide repeating units (at least 4), wherein leucine (being Xie Ansuan or Isoleucine once in a while) just occurs every 6 amino acid usually.Leucine zipper is extremely important for protein dimerization.Dimerization is precondition (Sessa etc. (1993) the EMBO J 12 (9): 3507-3517) of DNA combination, and can occur between two identical HDZip albumen (homodimer), perhaps between two different HDZip albumen (heterodimer).
The homeodomain gene is present in all eukaryotic cells, and constitutes the gene family of at least 89 members in the Arabidopis thaliana (Arabidopsisthaliana).Leucine zipper itself also sees except in the exophytic eukaryotic cell.But, it but is plant peculiar (finding at least 47 in 89 protein of Arabidopis thaliana) that homeodomain and leucine zipper exist simultaneously, and except vascular plant, also in moss (moss), run into (Sakakibara etc. (2001) Mol Biol Evol 18 (4): 491-502).Leucine zipper then is positioned at the C-end of homeodomain, these two element characteristics 3 amino acid that overlap each other.
Based on the sequence similarity standard, Arabidopis thaliana HDZip gene is divided into four different classifications: HDZip I to IV (Sessa etc. (1994) In Plant Molec Biol, 412-426 page or leaf).The same with other three classes HDZip albumen, the one-level amino acid structure aspects difference of I class HDZip albumen beyond homeodomain and leucine zipper is quite big.The feature of I class HDZip albumen also is two special characteristics in homeodomain and the leucine zipper:
1) in homeodomain, except constant amino acid Leu 16Trp 48Phe 49Asn 51Arg 53Outside, the 46th is Ala (A), and the 56th be Try (W) (perhaps once in a while for Phe (F)) (Sessa etc. (1997) J Mol Biol 274 (3): 303-309; See Fig. 1), this is called I class homeodomain, and
2) leucine zipper comprises 6 seven peptides, and except the pteridophyte Ceratopteris richardii that presents 7 seven peptides (position called after a, b, c, d, e, f and g position in each seven peptide, conservative leucine is in the d position; Sakakibara etc. (2001) Mol Biol Evol 18 (4): 491-502; See Fig. 2).HDZip II, III and IV present the only leucine zipper of 5 seven peptides of tool.
As for its DNA binding characteristic, I class HDZip albumen preferentially is combined in the overlapping 5bp half site of center position, i.e. CAA (A/T) ATTG (sessa etc. (1993) EMBO J 12 (9): 3507-3517).
Proved different HDZip albumen activation or check and transcribe.Utilized reporter gene (luciferase; Henriksson etc. (2005) Plant Phys 139:509-518) prove in Arabidopis thaliana: I class HDZip ATHBl ,-5 ,-6 and-16 works as activating transcription factor in the transient expression of Arabidopis thaliana leaf is measured.Utilize another reporter gene (glycuronidase; Meijer etc. (2000) MolGen Genet 263:12-21), prove that two kinds of rice I class HDZip albumen are that Oshox4 and Oshox5 work as incitant in the transient expression of rice cell suspension culture is measured.On the contrary, two kinds of rice II class HDZip albumen be Oshox1 with Oshox3 in identical test as transcription repressor (Meijer etc. (1997) the Plant J 11:263-276 that works; Meijer etc. (2000), the same).
Proved that multiple I class HDZip albumen participates in light and replys and participate in relevant the replying of dormin (ABA)/olighydria
Figure S200680049144XD00041
Deng (2003) Plant Cell Environ 26:1127-1136).Cross and express I class HDZip ATHBl ,-3 ,-13 ,-20 and-23 transgenic arabidopsis shows that these genes participate in regulation and control cotyledons and leaf is grown (Aoyama etc. (1995) Plant Cell 7:1773-1785; Hanson (2000) In Comprehensive summaries of Uppsala Dissertationsfrom the Faculty of Science and Technology, Uppsala).ATHB3 ,-13 ,-20 similar each other with-23 genes, and constitute the subclass of a uniqueness of I class HDZip.Owing in the time of these genome moulding expression, cause similar cotyledon change of shape, they be called as ace's leaf ( PoIntedcotyledon, POC) HDZip gene.The Hanson deduction in most cases also is correlated with in function aspects at closely-related I class HDZip albumen aspect system's generation.
Nitrate transport protein (NRT)
The set life of plant battalion, the resource of having to depend in the soil provides nutrient.Nitrogen in the soil exists mainly as amine salt or nitrate.The nitrogen of plant absorbs can be by means of three kinds of NO 3 -Absorption system carries out: NO 3 -The low-affinity movement system that concentration activates during greater than 1mM is at 1 μ M to 1mMNO 3 -The composing type of concentration and induction type high-affinity movement system.These three kinds of absorption systems are regulated and control in the mode of complexity.Nitrate just is transported to vacuole or is reduced into nitrite once absorption, and the latter is further metabolism of quilt in chloroplast(id) again successively.Nitrate also can be in apoplast (apoplasm) secretion again, or secrete to xylem and be transported to branch (shoot).Be responsible for the protein (NRT that nitrate absorbs in the root cells, nitrate transport protein) belongs to so-called main easily beggar's superfamily (MajorFacilitator Superfamily), comprise the protein that participates in the transhipment of small molecules solute, and length is generally 450 to 600 amino acid, has 12 membrane spaning domains.NRT albumen is classified as two family: NRT1 and NRT2 (Crawford ﹠amp; Glass, Trends Plant Sci.3,389-395,1998), and encoded by multigene family.NRT protein sequence high conservative, for example from the NRT2 albumen of moss with enjoy 60% sequence identity from the NRT2 albumen of dicotyledons, in the dicotyledons group, sequence identity between the NRT2 albumen is about 81%, and the sequence identity of unifacial leaf NRT2 albumen can be up to 89%.Orsel etc. have furtherd investigate the NRT2 family protein (Plant Physiol.129,886-896,2002) of Arabidopis thaliana.This family comprises 7 members, is distributed on three karyomit(e)s.Protein structure is conservative, and in 7 NRT2 albumen 5 preference in the root of nascent plant is expressed.With regard to structure, NRT2 albumen comprises the MFS_1 structural domain of crossing over protein about 90%, and the terminal membrane spaning domain of C-.MFS_1 structural domain itself comprises 10 or 11 membrane spaning domains by inference.
Think that NRT2 albumen is main relevant with the high-affinity movement system.In higher plant, this high-affinity absorption system it is believed that the double protein mixture that is subjected to be made up of NRT2 and NAR controls, and described NAR protein function it be unclear that (FEBS Letters 466 such as Zhou, 225-227; Plant such as Tong J.41,442-450,2005).Cross the ability expressing NRT2 and improve this high-affinity absorption system (Plant such as Fraisier J.23,489-496).NRT2 may also bring into play the function (Little etc., Proc.Natl.Acad.Sci.USA 102,13693-13698,2005) of nitrate susceptor.
Mori etc. had studied to cross and had expressed the transgenosis rice plants of rice NRT2 gene, and the seedling that shows nitrate hunger is compared with wild-type plant and has better NO 3 -Absorb.Good etc. (US20050044585) disclose nitrogen and have utilized the particularly transgenic plant of transaminase level rising of protein, and being in may be also may not be under the control of stress induced root-specific promoter.These plants show the nitrogen assimilated efficiency improves, but does not report the influence for the seed productive rate.In addition, this piece document also is disclosed in the plant to cross and expresses nitrate transport protein and do not bring favourable growth characteristics for these plants.In addition, not under the normal growth condition or with regard to complete plant life cycle studies, cross NRT albumen as yet.
Volume increase protein 16 (YEP16)
In YEP16 polypeptide and the F1F0-ATP synthase N-end structure territory of ATPase δ structural domain δ subunit enjoy certain similarity (relevant δ subunit details, consult InterPro IPR000711).
Shaggy sample kinases
Plant shaggy sample kinases is encoded by multigene family.Found that the arabidopsis gene group contains 10 shaggy sample kinases encoding genes, they are classified as four different subfamilies.Different family members' protein sequence is conservative at whole kinase domain camber, but N-and C-stub area difference are quite big, show that various plants shaggy sample kinases participates in diversified bioprocess, for example hormone signal conduction, growth and stress response.Based on the homology of protein sequence, plant shaggy sample kinases can be classified as four types (I-IV), wherein each type in four types all participates in different process (seeing Figure 13).Except can obtaining the kinase whose full length cDNA sequence of Arabidopis thaliana shaggy sample, can also in public database, obtain the kinase whose full length cDNA sequence of shaggy sample from colea (Brassica napus), alfalfa (Medicagosativa), tobacco (Nicotiana tabacum), rice (oryza sativa), petunia (Petunia hybrida) and Zea mays species such as (Zea mays).AtGSK1 is the kinase whose encoding gene of II type Arabidopis thaliana shaggy sample, has reported the responsive phenotype of salt of its compensation yeast calcineurin mutant.Shown that the kinase whose generation of same shaggy sample is induced by NaCl and dormin in the seedling.Stress response gene and anthocyanidin accumulation are induced in the excessive generation of having reported the AtGSK1 gene, and change positively charged ion level in the born of the same parents, thereby cause salt and drought tolerance to strengthen.
Consider the diversified bioprocess of the veriform known participation of shaggy sample kinases, we find that surprisingly it is stress response that two kinds of veriform shaggy sample kinases participate in same bioprocess.We find to be different from its alloytype shaggy sample kinases of II type unexpectedly can give the tolerance that plant increases abiotic stress.
Have now found that the expression of I class HDZip hox5 polypeptide or its homologue coding nucleic acid in the adjusting plant; Or regulate the expression of nitrate transport protein (NRT) in the plant or its homologue coding nucleic acid; Or YEP16 peptide coding expression of nucleic acids in the adjusting plant; Or regulate the expression of I type shaggy sample kinases in the plant or its homologue, make plant have the plant growth characteristics of improvement with respect to corresponding wild-type plant or other control plants.
Summary of the invention
Therefore the present invention provides the method for improvement plant growth characteristics, comprises the expression of regulating I class HDZip hox5 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of regulating NRT in the plant or its homologue coding nucleic acid; Or comprise and regulate YEP16 peptide coding expression of nucleic acids in the plant; Or comprise the expression of regulating I type shaggy sample kinases in the plant or its homologue.
Selecting suitable control plant is the conventional part that experiment arranges, and can comprise corresponding wild-type plant or not contain the corresponding plant of goal gene.Control plant is generally same plant species, perhaps in addition with plant to be assessed be same kind.Control plant can also be the invalid zygote (nullizygote) of plant to be assessed." control plant " not only refers to complete plant as used herein, and refers to plant part, comprises seed and plants subdivision.
Advantageously, implementing the method according to this invention produces with respect to the one or more aspects in plant, particularly gain in yield, growth improvement, biomass improvement, improved structure, cell fission improvement and the abiotic stress tolerance raising of corresponding wild-type plant or the improvement of other control plant growth characteristics.
Defined term in the literary composition " productive rate of increase " refers to the increase of following any one or many aspects, every kind all for corresponding wild-type or other control plants: (i) the one or more parts of plant, the biomass (weight) that increases of (can gather in the crops) part on the ground particularly, or the root biomass that increases, the root volume that increases, the radical amount that increases, the root diameter of increase or the root length of increase (thick root or radicula), or any other can be gathered in the crops the biomass that part increases; The (ii) seed overall yield of Zeng Jiaing, this comprises the increase of seed biomass (seed weight), and it can be seed weight increase on every plant or the single seed basis and/or the increase of per hectare or every acre of seed weight; The (iii) quantity of the paniculiform flower of each of Zeng Jiaing (Xiao Hua floret), it is expressed as the ratio that the full seed number accounts for the initial stage (primary) panicle number; The (iv) full rate of the seed of Zeng Jiaing (represent with per-cent, account for the ratio of Xiao Hua number for the full seed number); (v) (full) seed amount of Zeng Jiaing; (the vi) seed size of Zeng Jiaing, this also can influence the composition of seed; (the vii) seed volume of Zeng Jiaing, this also can influence the composition (total content and the composition that comprise oil, protein and sugar) of seed; (viii) (single or average) seed area of Zeng Jiaing; (ix) (single or average) seed length of Zeng Jiaing; (x) (single or average) seed width of Zeng Jiaing; (x) (single or average) seed girth of Zeng Jiaing; (xi) harvest index of Zeng Jiaing (HI), it is expressed as can gather in the crops part accounts for total biomass as the productive rate of seed ratio; (xii) thousand seed weight of Zeng Jiaing (TKW), this obtains by counting full seed quantity and gross weight extrapolation thereof.The TKW that increases can come from the increase of seed size and/or seed weight.The TKW that increases can also be from the increase of embryo size and/or endosperm size.
Be example with cereal, gain in yield can show as following one or more aspect: the increase of the plant quantity of per hectare or every acre of foundation, the increase of every plant grain ear quantity, the increase of line number, row grain number, grain weight, thousand seed weight, grain ear length/diameter, the increase of the full rate of seed (it multiply by 100 for the full seed number divided by the seed sum), etc.Be example with the rice, gain in yield can show as following one or more aspect: the panicle quantity of per hectare or every acre plant quantity, every plant, each paniculiform small ear quantity, each paniculiform flower (Xiao Hua) quantity (it is expressed as the full seed number and accounts for paniculiform ratio of initial stage), the increase of the full rate of seed (it multiply by 100 for the full seed number divided by the seed sum), the increase of thousand seed weight, etc.
Because transgenic plant of the present invention have the productive rate of increase, for the growth velocity of its life cycle respective stage, these plants may present the growth velocity (at least in its part life cycle) of increase with respect to control plant.The growth velocity that increases can be that one or more parts (comprising seed) of plant are distinctive, perhaps can spread all over whole strain plant basically.Have the plant that increases growth velocity and can have shorter life cycle.The life cycle of plant can be understood as expression and grows to the required time in ripe dry seeds stage that the plant generation is similar to parent material from ripe dry seeds.This life cycle can be subjected to the influence such as factors such as early stage vigor, growth velocity, green degree index, flowering time and seed maturity speed.The increase of growth velocity can appear at one or more stages in plant life cycle, perhaps appears in the process of whole plants life cycle basically.At the commitment in plant life cycle, the increase of growth velocity can reflect the vigor of enhancing.The increase of growth velocity can change the harvest cycle of plant, makes the plant can be than other possible situations more late sowing kind and/or faster results (similar effects can by Zao flowering time acquisition).If growth velocity fully increases, can allow to sow the more seed of same plant species (for example fully in the vegetative period of a routine, sowing and results rice plants, then sow and gather in the crops more rice plants).Similar with it, if growth velocity increases fully, can allow further to sow the seed (for example sow and gather in the crops rice plants, subsequently, for example, sow and optional results soybean, potato or any other suitable plant) of different plant species.Under the situation of some crop plants, also might be from the number of times of same rhizome results increase.The harvest cycle that changes plant can cause every acre year biomass yield increase (this be because (for example in 1 year) any specified plant can be grown and the increase of harvesting frequency).Compare with wild type counterparts, the increase of growth velocity can also be more wide region cultivation transgenic plant because the region restriction of planting plant during usually by plantation when (season early) or results (season in evening) hostile environment condition determined.If the shortening harvest cycle can be avoided this class unfavourable condition.Can obtain multiple parameter by growth curve, determine growth velocity, this class parameter can be: T-Mid (plant reaches the required time of its largest amount 50%) and T-90 (plant reaches the required time of its largest amount 90%), etc.
Implementing method of the present invention produces and the plant with growth velocity of increase.Therefore, the invention provides the method that increases plant growth rate, described method comprises the expression of regulating I class HDZiphox5 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of regulating NRT in the plant or its homologue coding nucleic acid; Or comprise and regulate YEP16 peptide coding expression of nucleic acids in the plant; Or comprise the expression of regulating I type shaggy sample kinases in the plant or its homologue.
No matter plant is under the non-stress conditions, or plant with respect to multiple the coercing of control plant contact the increase of productive rate and/or growth velocity takes place all.Usually plant is replied by growth more slowly and coerces contact.Under the severe stress conditions, plant even can stop growing fully.On the other hand, slightly coerce to be defined as in the text and when plant contact, do not cause the plant forfeiture that stops growing fully to restart any of energy for growth and coerce.The growth that slightly coercing on the meaning of the present invention causes coercing plant is compared with the control plant under the non-stress conditions, under be reduced to 40%, 35% or 30%, preferably be lower than 25%, 20% or 15%, more preferably less than 14%, 13%, 12%, 11% or 10% or lower.Because the development of the farming method (irrigation, fertilising, pesticide treatments), the crop plants of cultivation usually can not run into severe and coerce.Therefore, the impaired growth by slight stress-inducing becomes the factor of not expecting in the agricultural usually.Slightly coercing can be that daily biological and/or abiotic (environment) of plant contact coerced.Abiotic stress can because of arid or excessive water, anaerobic be coerced, salt stress, chemical toxicity, oxidative stress and heat, cold or freezing temperature cause.Abiotic stress can be to coerce the osmotic stress that causes because water is coerced (particularly because arid), salt stress, oxidative stress or ion.It generally is that those that caused by pathogenic agent such as bacterium, virus, fungi and insect are coerced that biology is coerced.
Implementing the method according to this invention causes plant abiotic stress to be had the tolerance of increase.As (Planta (2003) 218:1-14) such as Wang reported, abiotic stress caused that a series of morphology, physiology, biological chemistry and molecule change, and cause disadvantageous effect to plant-growth and productivity.Known arid, salinity, extreme temperature and oxidative stress connect each other, and can pass through similar mechanism induced growth and primary cellular defect.For example, arid and/or salinity mainly show as osmotic stress, cause destroying stable state and ion distribution in the cell.Oxidative stress accompanies with high temperature or low temperature, salinity or drought stress usually, can cause the sex change of function and structural protein.So these diversified environment-stress activate similar cell signaling path and cell response usually, for example generation of stress protein, the rise of antioxidant, the accumulation of compatible solutes and growth are prevented.
Since diversified environment-stress activates similar path, the present invention illustrates (under the situation of the purposes that the present invention relates to I class HDZip hox5 polypeptide and coding nucleic acid thereof) and should not be considered as being confined to drought stress about drought stress, and should be considered as more showing that I class HDZip hox5 polypeptide or its homologue participate in the screen display information (screen) of abiotic stress in general.In addition, method of the present invention can implemented under the non-stress conditions or under the slight drought condition, so that the plant that has the growth characteristics (the particularly productive rate of Zeng Jiaing) of improvement with respect to corresponding wild-type or other control plants to be provided.Term used herein " non-coercing " condition optimization run into for the plant most probable those significantly do not deviate from condition and other abiotic conditions of usual weather, be preferably those conditions that allow the plant optimum growh.Those skilled in the art know normal edaphic condition and the weather condition in given place.
There is " dialogue " (Rabbani etc. (2003) Plant Physiol 133:1755-1767) of special high level in report between drought stress and high salinity are coerced.Therefore, obviously I class HDZiphox5 polypeptide or its homologue are given the purposes of plant drought tolerance together with it, also will resist finding a place where one can give full play to one's talent aspect multiple other abiotic stress at protective plant.Similarly, obviously I type shaggy sample kinases (as herein defined) is given the purposes of plant salt tolerance together with it, also will resist finding a place where one can give full play to one's talent aspect multiple other abiotic stress at protective plant.In addition, Rabbani etc. (2003, Plant Physiol 133:1755-1767) report exists stress tolerance and the similar molecular mechanism of replying between dicotyledonous and monocotyledons.Therefore method of the present invention can advantageously be applied to any plant.
Term " abiotic stress " is interpreted as being expressed as follows one or more aspects as herein defined: water coerces that (because arid or excessive water), anaerobic are coerced, salt stress, temperature are coerced (because hot, cold or freezing temperature), chemical toxicity is coerced and oxidative stress.According to an aspect of the present invention, abiotic stress is osmotic stress, is selected from that water is coerced, salt stress, oxidative stress and ion coerce.It is drought stress that preferably water is coerced.The term salt stress is not limited to common salt, and can be following any or multiple: NaCl, KCl, LiCl, MgCl 2, CaCl 2Etc..
The abiotic stress tolerance that increases shows as the plant yield that increases and decreases under the abiotic stress condition.Particularly under the situation of the purposes that the present invention relates to I class HDZip hox5 polypeptide and coding nucleic acid thereof, the productive rate of this type of increase can comprise following one or more aspect: each the paniculiform full rate of seed, the HI of increase, the TKW of increase, the root length of increase or root diameter of increase of spending number, increasing of the full seed number of increase, the seed overall yield of increase, increase, wherein each aspect is all for corresponding wild-type plant.
Implementing method of the present invention provides the plant of the abiotic stress tolerance with increase.Implementing method of the present invention provides for the corresponding wild-type plant of growing under suitable condition or other control plants under the non-stress conditions or the plant with improvement growth characteristics (particularly productive rate of Zeng Jiaing) that grows under the slight drought condition.
According to the present invention, the method that increases the plant abiotic stress tolerance is provided, described method comprises the expression of regulating I class HDZip hox5 polypeptide in the plant or its homologue coding nucleic acid.According to an aspect of the present invention, abiotic stress is osmotic stress, is selected from following one or more aspect: water is coerced, salt stress, oxidative stress and ion are coerced.It is drought stress that preferably water is coerced.
The present invention also provides the method that improves the plant abiotic stress tolerance, comprise the activity that increases I type shaggy sample kinases in the plant or its homologue, described I type shaggy sample kinases has: (i) with the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/N CXXX G/A/S, wherein X can be arbitrary amino acid.
The present invention also provides the method for improvement in the growth characteristics (particularly increasing productive rate) of growing plants under the non-stress conditions or under the slight drought condition, and described method comprises the expression (particularly increasing its expression) of regulating NRT polypeptide in the plant or its homologue coding nucleic acid.In the preferred embodiment of the invention, the method according to this invention issues the increase of productivity and/or growth velocity at non-stress conditions.
Particularly under the situation of the purposes that the present invention relates to I class HDZip hox5 polypeptide and coding nucleic acid thereof, implement method of the present invention and cause green degree index to increase with respect to corresponding wild-type plant.Green degree index is the ratio (being expressed as %) of yellow pixel in the plant image of digital camera record as herein defined.The green degree index that increases can show the aging that reduces or postpone, and this can prolong the photosynthesis of plants activity successively, and then brings multiple beneficial effect well-known in the art.
Therefore the present invention provides the method that increases the green degree index of plant, and described method comprises the expression of regulating I class HDZip hox5 polypeptide in the plant or its homologue coding nucleic acid.Preferred green degree index increases under the abiotic stress condition, more preferably under the water stress conditions, also preferably under the drought stress condition.
Comprise in method of the present invention and to regulate under the situation that I class HDZip hox5 polypeptide in the plant or its homologue coding nucleic acid express, the preferred productive rate that increases comprises following one or more aspect: each the paniculiform full rate of seed, the HI of increase, the TKW of increase, the root length of increase or root diameter of increase of spending number, increasing of the full seed number of increase, the seed overall yield of increase, increase, wherein each aspect is all for corresponding wild-type or other control plants.
The preferred aspect according to the present invention provides the method that increases plant yield with respect to corresponding wild-type or other control plants, and described method comprises the expression of regulating I class HDZip hox5 polypeptide in the plant or its homologue coding nucleic acid.
Comprise in method of the present invention and to regulate under the situation that NRT in the plant or its homologue coding nucleic acid express that the preferred plant that produces has the productive rate of increase, and more particularly has the biomass of increase and/or the seed productive rate of increase.The preferred seed productive rate that increases comprises the increase of following one or more aspects: (full) seed amount, seed gross weight, seed size, thousand seed weight and harvest index, wherein each aspect is all for corresponding wild-type or other control plants.
According to the present invention another preferred aspect provides the method that increases plant yield, and described method comprises the expression of regulating NRT polypeptide in the plant or its homologue coding nucleic acid (preferably increase its active and/or express).
Comprise under the situation of regulating YEP16 peptide coding expression of nucleic acid in the plant that in method of the present invention the productive rate that preferably increases or improve is the seed productive rate of raising for corresponding wild-type plant seed productive rate.
Therefore another preferred aspect according to the present invention provides the method that increases the seed productive rate with respect to corresponding wild-type or other control plants, comprises the expression of regulating YEP16 polypeptide in the plant or its homologue coding nucleic acid.
Comprise in method of the present invention and to regulate under the situation that I type shaggy sample kinases in the plant or its homologue coding nucleic acid express that the growth characteristics of preferred improvement are the abiotic stress tolerances that improves.
Another preferred aspect according to the present invention, the method that improves the plant abiotic stress tolerance is provided, comprise that the described I type shaggy sample kinases of the expression of regulating I type shaggy sample kinases in the plant or its homologue coding nucleic acid (preferably increase its active and/or express) has (i) and the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147 and (ii) motif I:R/H/V/N/Q E/GLK G/N and motif II:K Q/N CXXX G/A/S, wherein X can be arbitrary amino acid.
Therefore can advantageously use method of the present invention to any plant.
Term used herein " plant " comprises ancestors and offspring and the plant part of whole strain plant, plant, comprises seed, branch, stem, leaf, root (comprising stem tuber), flower and tissue and organ, wherein above-mentioned each all comprise goal gene/nucleic acid.Vegetable cell, suspension culture, callus, embryo, meristem zone, gametophyte, sporophyte, pollen and sporule also contained in term " plant ", equally wherein above-mentioned each all comprise goal gene/nucleic acid.
The plant that is particularly useful in the methods of the invention comprises that all belong to the plant of vegitabilia (Viridiplantae) superfamily, particularly unifacial leaf and dicotyledons comprise being selected from following feed or feed leguminous plants, ornamental plant, food crop, arbor or shrub: Acacia species (Acacia spp.), maple species (Acer spp.), Actinidia species (Actinidia spp.), Aesculus species (Aesculus spp.), New Zealand kauri (Agathis australis), Albizia amara, three look spinulose tree ferns (Alsophila tricolor), Andropogon species (Andropogon spp.), Arachis species (Arachis spp), betel nut (Areca catechu), Asteliafragrans, the Radix Astragali (Astragalus cicer), Baikiaea plurijuga, Betula species (Betula spp.), Btassica species (Brassica spp.), Bruguiera conjugata (Bruguiera gymnorrhiza), Burkea africana, palas (Butea frondosa), Cadaba frinosa, Zhu Ying Pittosporum species (Calliandra spp), tea (Camellia sinensis), Canna generalis Bailey (Canna indica), Capsicum species (Capsicum spp.), Cassia species (Cassia spp.), Centrosema (Centroema pubescens), Chaenomeles species (Chaenomeles spp.), Chinese cassia tree (Cinnamomum cassia), fruitlet coffee (Coffea arabica), Colophospermummopane, variation coronule flower (Coronillia varia), Chinese holly (Cotoneaster serotina), hawthorn species (Crataegus spp.), Cucumis species (Cucumis spp.), Cupressus species (Cupressusspp.), Cyathea dealbata, oblonga (Cydonia oblonga), ball cryptomeria (Cryptomeriajaponica), Cymbopogon species (Cymbopogon spp.), Cynthea dealbata, oblonga (Cydoniaoblonga), Dalbergia monetaria, Da Ye Rhizome of Fortune's Drynaria (Davallia divaricata), acutifoliate podocarpium herb species (Desmodium spp.), Di Kalan (Dicksonia squarosa), Diheteropogonamplectens, Dioclea spp, sickle Dolichos species (Dolichos spp.), Dorycnium rectum, awl fringe barnyard grass (Echinochloa pyramidalis), Ehrartia spp. Finger-millet (Eleusine coracana), Eragrestis spp., Erythrina species (Erythrina spp.), eucalyptus species (Eucalyptus spp.), Euclea schimperi, Jin Mao (Fulalia villosa), Fagopyrum species (Fagopyrum spp.), Fei Yueluo (Feija sellowiana), grass thunder species (Fragaria spp.), Moghania species (Flemingia spp), Freycinetia banksii, Geranium thunbergii, ginkgo (Ginkgobiloba), Glycine javanica, Gliricidia spp, upland cotton (Gossypium hirsutum), Grevillea species (Grevillea spp.), Guibourtia coleosperma, rock Astragalus species (Hedysarum spp.), Hemarthria compressa (Hemarthia altissima), turn round Huang Mao (Heteropogoncontortus), barley (Hordeum vulgare), Hyparrhenia rufa, little fructus forsythiae (Hypericumerectum), Hyperthelia dissoluta, spend front yard indigo plant (Indigo incarnata) in vain, Jris species (Iris spp.), Leptarrhena pyrolifolia, lespedeza species (Lespediza spp.), Lactuca species (Lettuca spp.), Leucaena leucocephala, Loudetia simplex, Lotonusbainesii, Lotus species (Lotus spp.), sclerderm beans (Macrotyloma axillare), Malus species (Malus spp.), Manihot esculenta, alfalfa (Medicago sativa), metasequoia (Metasequoia glyptostroboides), plantain (Musa sapientum), Nicotiana species (Nicotianum spp.), donkey eats careless species (Onobrychis spp.), Ornithopus spp., Oryza species (Oryza spp.), African peltophorum (Peltophorum africanum), Pennisetum species (Pennisetum spp.), avocado (Persea gratissima), green winter Solanum species (Petunia spp.), Phaseolus species (Phaseolus spp.), betel nut bamboo (Phoenix canariensis), Phormiumcookianum, Photinia species (Photinia spp.), white spruce (Picea glauca), Pinus species (Pinus spp.), pea (Pisum sativum), alpine totara (Podocarpus totara), Pogonarthria fleckii, Pogonarthria squarrosa, Populus species (Populus spp.), algarroba (Prosopis cineraria), Pseudotsuga menziesii (Mirbel) Franco (Pseudotsuga menziesii), Pterolobiumstellatum, European pear (Pyrus communis), oak species (Quercus spp.), Rhaphiolepsisumbellata, delicious rod is spent palm fibre (Rhopalostylis sapida), Rhus natalensis, Europe gooseberry (Ribes grossularia), currant species (Ribes spp.), acacia (Robinia pseudoacacia), rose species (Rosa spp.), rubus species (Rubus spp.), Salix species (Salix spp.), Schyzachyrium sanguineum, parasol pine (Sciadopitys verticillata), sequoia sempervirens (Sequoia sempervirens), big tree (Sequoiadendron giganteum), dichromatism chinese sorghum (Sorghum bicolor), spinach species (Spinacia spp.), Sporobolus fimbriatus, Stiburus alopecuroides, Stylosanthos humilis, Triquetrous Tadehagi Herb species (Tadehagi spp), bald cypress (Taxodium distichum), Arabic Herba Themedae japonicae (Themeda triandra), Clover species (TRifolium spp.), Triticum species (Triticum spp.), tsuga heterophylla (Tsugaheterophylla), genus vaccinium species (Vaccinium spp.), Vetch species (Vicia spp.), grape (Vitis vinifera), the fertile gloomy flower (Watsonia pyramida) of awl fringe, common calla (Zantedeschiaaethiopica), Zea mays (Zea mays), Amaranthus (amaranth), arithoke (artichoke), Asparagus (asparagus), cabbage (broccoli), brassica oleracea var gemmifera (Brussels sprouts), wild cabbage, rape (canola), Radix Dauci Sativae, Cauliflower, celery, kale (collard greens), flax, kale (kale), Lens culinaris belongs to (lentil), Semen Brassicae campestris rape (oilseed rape), gumbo (okra), onion, potato, rice, soybean, strawberry, beet, sugarcane, Sunflower Receptacle, tomato, pumpkin (squash), tea and algae etc.
According to the preferred embodiment of the invention, plant is crop plants such as soybean, Sunflower Receptacle, rape, clover, Semen Brassicae campestris, cotton, tomato, potato or tobacco.Also preferred plant is monocotyledons such as sugarcane.More preferably plant is cereal, for example rice, corn, wheat, barley, grain, rye, Chinese sorghum or oat.
The I class HDZip hox5 polypeptide and the homologue I class HDZip hox5 thereof that can be used for the inventive method Nucleic acid/gene
Term " I class HDZip hox5 polypeptide or its homologue " refers to such polypeptide as herein defined, and it comprises to the C-end from the N-end: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5.
In addition, I class HDZip hox5 polypeptide or its homologue can comprise following arbitrary or both have both at the same time: (a) Trp tail; (b) RPFF amino acid motif, wherein R is Arg, P is Pro, and F is Phe.In this motif, allow to carry out the one or more conservative change on the optional position, and/or one or two the non-conservative change on the optional position.When end was studied protein from the N-end to C-, (b) motif in was positioned at before the acid box.
An example of I class HDZip hox5 polypeptide as hereinbefore defined comprises to C-terminal from the N-end: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5; And comprise in addition: (a) Trp tail; (b) RPFF amino acid motif, wherein R is Arg, P is Pro, and F is Phe, shown in SEQ ID NO:2.How such example provides in this paper embodiment 1 Table A.
I class HDZip hox5 polypeptide or its homologue are by I class HDZip hox5 nucleic acid/genes encoding.Therefore term " I class HDZip hox5 nucleic acid/gene " is encode the I class HDZip hox5 polypeptide of definition as mentioned or any nucleic acid/gene of its homologue as herein defined.
Utilize routine techniques well-known in the art as by sequence alignment, can easily identify I class HDZip hox5 polypeptide or its homologue.It is well-known in the art being used for sequence alignment method relatively, and these class methods comprise GAP, BESTFIT, BLAST, FASTA and TFASTA.GAP utilizes Needleman and Wunsch ((1970) J.Mol.Biol.48; Algorithm 443-453) is sought the comparison that can make the maximization of coupling number and make minimized two complete sequence of room number.BLAST algorithm (Altschul etc. (1990) J.Mol.Biol.215:403-10) sequence of calculation identity per-cent, and the similarity between statistical study two sequences.The software of implementing the BLAST analysis can obtain publicly by NCBI.For example, use is available from the ClustalW multiple sequence alignment algorithm (1.83 editions) of http://clustalw.genome.jp/sit-bin/nph-ClustalW, adopt default comparison parameter in twos and per-cent methods of marking, can easily identify the I class HDZip hox5 homologue of the leucine zipper that comprises I class homeodomain and tool seven peptides more than 5.Can carry out trickle edit, to optimize the comparison between conservative motif, this will be apparent for those skilled in the art.
Can utilize specialized database to identify the multiple structural domain of I class HDZip hox5 albumen, as homeodomain and leucine zipper, for example: SMART (Schultz etc. (1998) Proc.Natl.Acad.Sci.USA 95,5857-5864; Letunic etc. (2002) Nucleic Acids Res 30,242-244; Http:// smart.embl-heidelberg.de/), InterPro (Mulder etc., (2003) Nucl.Acids.Res.31,315-318; Http:// www.ebi.ac.uk/interpro/), Prosite (Bucher and Bairoch (1994), A generalized profile syntax for biomolecular sequencesmotifs and its function in automatic sequence interpretation. (In) ISMB-94; Proceedings 2nd International Conference on Intelligent Systems forMolecular Biology.Altman R., Brutlag D., Karp P., Lathrop R., Searls D writes, the 53-61 page or leaf, AAAIPress, Menlo Park; Hulo etc., Nucl.Acids.Res.32:D134-D137, (2004), Http:// www.expasy.org/prosite/) or Pfam (Bateman etc., Nucleic Acids Research 30 (1): 276-280 (2002), Http:// www.sanger.ac.uk/Software/Pfam/).Can utilize specialized database to carry out leucine zipper prediction and the evaluation of seven peptides, as 2ZIP, this database is with the simulation of standard coiled coil prediction algorithm and characteristic leucine repeating unit search (Bornberg-Bauer etc. (1998) the Computational Approaches to Identify Leucine Zippers that combines, Nucleic Acids Res., 26 (11): 2740-2746 Http: // 2zip.molgen.mpg.de).
In addition, also can easily identify the existence of acid box.Can be used to form (representing with %) from the software program first order calculation amino acid of ExPASy server, to determine whether the polypeptide structure territory is rich in specific amino acid, ProtParam instrument (2003) ExPASy:the proteomics server for in-depth protein knowledge and analysis.NucleicAcids Res 31:3784-3788 such as () Gasteiger E particularly.The composition of target protein matter and the average amino acid composition (representing with %) in the Swiss-Prot protein sequence database can be compared then.In this Swiss-Prot database, average A sp (D) and Glu (E) content are respectively 5.3% and 6.6%, and the combination average value is 11.9%.As an example, the acid box of SEQ ID NO:2 contains 9.1% D and 54.5% E, and the combination average value is 63.6%.Such as herein defined, acidity is rich in box and has combination Asp (D) and Glu (E) content (representing with %) that is higher than the average amino acid composition of protein (representing with %) in the Swiss-Prot protein sequence database.Acid box can be the part of transcriptional activation domain.According to the aminoacids content in eukaryotic transcription activation structure territory it is classified, and main classification comprises that acidity, glutamine are rich in proline(Pro) and is rich in activation structure territory (Rutherford etc. (2005) Plant is (5): 769-88 J.43, and wherein reference).
A part of protein in I class HDZip hox5 polypeptide or its homologue also comprises the RPFF amino acid motif, and wherein R is Arg, and P is Pro, and F is Phe.In this motif, allow to carry out the one or more conservative change on the optional position, and/or one or two the non-conservative change on the optional position.When end was studied protein from the N-end to C-, this motif was positioned at acid box (see figure 2) before.Can utilize the existence that sequence alignment method is relatively identified RPFF that is used for mentioned above.In some cases, can adjust default parameter, with the strict degree of change search.For example utilize BLAST, can increase the statistical significance threshold value (being called " expection " value, i.e. the E value) for report database sequences match event, to show the match event of low strict degree.Can identify the short event of almost accurately mating in this way.
A part of protein in I class HDZip hox5 polypeptide or its homologue also comprises the Trp tail.The Trp tail is last 10 amino acid (see figure 2)s that protein C-end comprises at least one Trp residue as herein defined.
The example of I class HDZip hox5 polypeptide or its homologue (by the coding of the polynucleotide sequence accession number in the bracket) provides in Table A.
It should be understood that the sequence that falls into " I class HDZip hox5 polypeptide or its homologue " definition is not limited to the given aminoacid sequence of Table A, but any such polypeptide, it comprises to C-terminal from the N-end: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5 all is applicable to the method for the present invention of implementing.
I class HDZip hox5 polypeptide or its homologue have dna binding activity, preferred combination is at the overlapping 5bp half site of center position, be CAA (A/T) ATTG, as detected in the yeast one-hybrid test (Meijer etc. (2000) Mol Gen Genet 263:12-21).In the instantaneous measurement of rice cell suspending liquid, to bombard I class HDZip hox5 polypeptide jointly with gus reporter gene and cause the dyeing speck number to increase, these spot colors are darker (Meijer etc., the same) also.This mensuration can be used for proving the incitant function of I class HDZip hox5 polypeptide or its homologue.
The NRT polypeptide and homologue and their nucleic acid of coding that can be used for the inventive method
Term " NRT or its homologue " refers to such polypeptide as herein defined, and it comprises (i) MFS_1 structural domain (Pfam accession number PF07690, InterPro accession number IPR011701), the (ii) membrane spaning domain of following.Fig. 6 has provided an example.It is active in the high-affinity nitrate transport that preferred NRT albumen or its homologue have NRT, and do not contain PTR2 structural domain (Pfam accession number PF00854, InterPro accession number IPR000109).
Preferred NRT albumen or its homologue comprise sequence label 1 (SEQ ID NO:57):
(N/S)(Y/P)(T/G/S/A)W(I/V/L)(F/L/T)(V/A/F/L)(L/V/M/I)(L/T/I/A/N)YG(Y/F)(S/C/T)(M/F/Y)G(V/I)EL(T/S)(T/I/V)(D/G/N)N(V/I/N)(I/V)(A/S/H/V)(E/Q/G)Y。
Also preferred NRT albumen or its homologue comprise as the next item down or multinomial:
Sequence label 2 (SEQ ID NO:58):
LG(P/A)RYG(C/T)AF(L/S)；
Sequence label 3 (SEQ ID NO:59):
STFAA(A/R)PL(V/I)(P/V)(I/L/V)IR(D/E)NL(N/D)(L/P)；
Sequence label 4 (SEQ ID NO:60):
VRF(L/M)IGF(S/C)LA；
Sequence label 5 (SEQ ID NO:61):
FVSC(Q/R)YW(M/T)S(T/V)(M/S)(F/M)。
More preferably NRT albumen or its homologue comprise as the next item down or multinomial:
Sequence label 6 (SEQ ID NO:62):
K(A/Q/S/M/H/T)D(I/V)GNAGVASV(S/T)G(S/A)I(F/L)SR(L/G)；
Sequence label 7 (SEQ ID NO:63):
NG(L/T/C)A(A/G)GWG；
Sequence label 8 (SE ID NO:64):
G(A/S)G(L/V/Q)TQ(L/P)(L/V/I)(F/E)F(T/S/D)(S/T)(S/A/T)。
Most preferably NRT albumen is shown in SEQ ID NO:53.
Membrane spaning domain is about 15 to 30 amino acid, and is made of the hydrophobic residue that forms the α spiral usually.They normally based on hydrophobicity prediction (Klein etc. for example, Biochim.Biophys.Acta 815,468,1985; Or Sonnhammer etc., In J.Glasgow, T.Littlejohn, F.Major, R.Lathrop, D.Sankoff and C.Sensen write, Proceedings ofthe SixthInternational Conference on Intelligent Systems for Molecular Biology, 175-182 page or leaf, Menlo Park, CA, 1998.AAAI Press).
Alternatively, the homologue of NRT albumen has 50% according to the amino acid shown in the preferred sequence that increases progressively and the SEQ ID NO:53,55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% overall sequence identity.Overall sequence identity utilizes the overall comparison algorithm to determine, for example (GCG Wisconsin software package, Accelrys) the Needleman Wunsch algorithm in preferably adopts default parameter to the GAP program.
Can utilize specialized database to identify the multiple structural domain of NRT albumen, for example SMART (Schultz etc. (1998) Proc.Natl.Acad.Sci.USA 95,5857-5864; Letunic etc. (2002) Nucleic Acids Res 30,242-244; Http:// smart.embl-heidelberg.de/) InterPro (Mulder etc., (2003) Nucl.Acids.Res.31,315-318; Http:// www.ebi.ac.uk/interpro/), Prosite (Bucher and Bairoch (1994), Ageneralized profile syntax for biomolecular sequences motifs and itsfunction in automatic sequence interpretation. (In) ISMB-94; Proceedings2nd International Conference on Intelligent Systems for Molecular Biology.Altman R., Brutlag D., Karp P., Lathrop R., Searls D writes, the 53-61 page or leaf, AAAIPress, Menlo Park; Hulo etc., Nucl.Acids.Res.32:D134-D137, (2004), Http:// www.expasy.org/prosite/) or Pfam (Bateman etc., Nucleic AcidsResearch 30 (1): 276-280 (2002), Http:// www.sanger.ac.uk/Software/Pfam/).
The method of search and evaluation NRT homologue falls into limit of power of those skilled in the art and so on fully.These class methods comprise sequence available in the sequence of computer-reader form SEQ ID NO:1 or 2 representatives and the public database are compared, as MIPS ( Http:// mips.gsf.de/), GenBank ( Http:// www.ncbi.nlm.nih.gov/Genbank/index.html) or EMBL Nucleotide database ( Http:// www.ebi.ac.uk/embl/index.html), use sequence alignment well known in the art or comparison algorithm, as GAP (Needleman and Wunsch, J.Mol.Biol.48; 443-453 (1970)), BESTFIT (uses local homology's algorithm (Advances in Applied Mathematics 2 of Smith and Waterman; 482-489 (1981))), BLAST (Altschul, S.F., Gish, W., Miller, W., Myers, E.W.﹠amp; Lipman, D.J., J.Mol. Biol.215:403-410 (1990)), FASTA and TFASTA (W. R.Pearson and D.J.LipmanProc.Natl.Acad.Sci.USA 85:2444-2448 (1988)).The software of carrying out the BLAST analysis can obtain publicly from NCBI (NCBI).
The example of protein that falls into " NRT polypeptide or its homologue " definition comprises the rice proteinoid and from the protein of other species, as Zea mays, reed (Phragmites australis), barley (Hordeum vulgare), wheat (Triticum aestivum), colea, tomato (Lycopersiconesculentum), tobacco, Radix Dauci Sativae (Daucus carota), trembling poplar (Populus tremulus), Lotus japonica, peach (Prunus persica), soybean (Glycine max) and Arabidopis thaliana, etc.The non-limiting tabulation of NRT albumen example provides in the Table I of this paper embodiment 14.
But, consider to be used for method of the present invention from the other plant classification equally as the NRT albumen of moss or pteridophyte.For example, moss small liwan moss (Physcomitrellapatens) has at least 5 kinds of NRT albumen (GenBank accession number BAD00097, BAD00098, BAD00099, BAD00100, BAD00101).
Be to be understood that, term " NRT polypeptide or its homologue " be not limited to shown in the SEQ ID NO:53 or Table I in given sequence, but any polypeptide that satisfies following standard: comprise functional MFS 1 structural domain, with the conservative sequence label of one or more SEQ ID NO:57 to 64, and the membrane spaning domain that is positioned at MFS_1 domain C defined above-end; Perhaps the sequence with SEQ IDNO:53 has at least 50% sequence identity, all is applicable to the method for the present invention of implementing.
Be to determine the translocator activity of NRT, adopt the described nitrate absorption measurement of Tong etc. (Plant J.41,442-450,2005).In brief, in Xenopus laevis (Xenopus) ovocyte, express purpose NRT albumen, and measure 15The absorption of N enrichment nitrate.If desired, can coexpression nar2 gene, to increase nitrate transport.
Alternatively, the activity of NRT albumen or its homologue can be measured by express NRT polypeptide or its homologue be under the control of GOS2 promotor in rice growing kind Japan fine (Oryza sativa cultivar Nipponbare), and this will produce the plant of the seed productive rate of comparing ground biomass with increase and/or increase with corresponding wild-type plant.The increase of this seed productive rate can be weighed in several ways, for example increase of seed gross weight, full seed quantity or seed sum, the increase of the increase of harvest index or each panicle flower.
NRT albumen or its homologue are by NRT nucleic acid/genes encoding.Therefore term " NRT nucleic acid/gene " is encode the NRT albumen of definition as mentioned or any nucleic acid/gene of its homologue as herein defined.
The YEP16 polypeptide and homologue and their nucleic acid of coding that can be used for the inventive method
Term " YEP16 polypeptide " refers to the sequence of SEQ ID NO:128.The homologue of YEP16 polypeptide refers to enjoy according to the aminoacid sequence of the preferred sequence that increases progressively and SEQ ID NO:128 any aminoacid sequence of at least 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% sequence identity.Therefore this paper " YEP16 polypeptide or its homologue coding nucleic acid " addressed any nucleotide sequence of the YEP16 polypeptide of definition as mentioned that refers to encode, or coding is any nucleic acid of the YEP16 homologous peptide thing of definition as mentioned.
The I type shaggy sample kinases and homologue and their nucleic acid of coding that can be used for the inventive method
Term " I type shaggy sample kinases or its homologue " refers to such polypeptide as herein defined, and it has: (i) with the sequence identity of aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/N CXXXG/A/S, wherein X can be arbitrary amino acid.The polypeptide that satisfies aforementioned requirement can make I type shaggy sample kinases be different from its alloytype shaggy sample kinases.
Can utilize routine techniques well known to those skilled in the art easily to identify and fall into " I type shaggy sample kinases or its homologue " defined above.For example, can easily determine and the polypeptide of amino acid with at least 77% identity shown in the SEQ ID NO:147 by sequence alignment.It is well-known in the art being used for sequence alignment method relatively, and these class methods comprise GAP, BESTFIT, BLAST, FASTA and TFASTA.GAP utilizes Needleman and Wunsch (J.Mol.Biol.48; 443-453,1970) algorithm is sought the comparison that can make the maximization of coupling number and make minimized two complete sequence of room number.BLAST arithmetic calculation sequence identity per-cent, and the similarity between statistical study two sequences.The software of implementing the BLAST analysis can obtain publicly by NCBI.For example, utilize VNTI AlignX multiple ratio to program, based on improved clustal W algorithm (InforMax, Bethesda, MD, Http:// www.informaxinc.com), adopt default setting, the open point penalty in room be 10 and the room extend to 0.05, compare (for example referring to comparison shown in Figure 14) by inquiring about aminoacid sequence and known I type shaggy sample kinase sequence, can easily identify the shaggy sample kinases or its homologue that have at least 77% identity with the amino acid shown in the SEQ ID NO:147.
Those skilled in the art also can easily identify such sequence, and it has motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/N CXXX G/A/S, and wherein X can be arbitrary amino acid.This can be by comparing and searching for the homology zone and realize.
Following table 1 has shown motif I:R/H/V/N/Q E/GLK G/N and the motif II:K Q/N CXXX G/A/S (wherein X can be arbitrary amino acid) that finds in the sequence of SEQ ID NO:147, and corresponding motif in the homologous sequence.Overall identity per-cent shown in the table 1 is that SEQ ID NO:147 is compared (full length sequence is to full length sequence) with the accession number shown in the table.
The conservative motif of finding in table 1:I type shaggy sample kinases and the homologue thereof
Accession number: I type shaggy sample kinases The gene title Motif 1 Motif 2 Overall identity % (EMBOSS) with SEQ ID NO:147
BAB40983.1 (SEQ ID NO：147) Rice OSK γ HELKG KQCSYAG -
AY103545 Corn HELKG KQCSYVG 94.4％
AY108486 Corn HELKN KQCAFVG 84.4％
AAM77397.1 Wheat HELKG KNCAFVG 80.9％
CAA48538.1， At5g26750 Arabidopis thaliana ASK α HELKG KQCPWLG 85.6％
CAA48474.1 Clover MSK-1 HELKG KQCPFLG 83.5％
CAA04265 Arabidopis thaliana Ask α HGLKG KQCPWLG 84.6％
CAA54803.1 Tobacco NtK1 HELKG KQCPSLG 85.3％
CAA48472.1 Clover MSK3 NELKG KQCALFG 85.0％
CAA48473.1 Clover MSK2 VELKG KQCSLFA 82.0％
CAA58594.1 Petunia Shaggy4 QELKG KQCTFLG 81.5％
At5g14640 Arabidopis thaliana ASK ε QELKG KQCSFLA 83.9％
At3g05840 Arabidopis thaliana ASK γ HELKG KQCPWLS 84.6％
CAA04265 Rice shaggy α HGLKG KQCPWLG 84.6％
AK058276 Rice shaggy HELKG KQCAFVG 83.2％
AK099599 Rice Shaggy RELKG KQCAFLG 78.5％
Its alloytype shaggy sample
At4g18710 Arabidopis thaliana Ask η Do not have Do not have 70.0％
At2g30980 Arabidopis thaliana Ask ζ Do not have Do not have 72.9％
At4g00720 Arabidopis thaliana Ask θ Do not have Do not have 64.1％
At1g57870 Arabidopis thaliana Ask δ Do not have Do not have 73.4％
At1g09840 Arabidopis thaliana Ask κ Do not have Do not have 72.4％
At1g06390 Arabidopis thaliana Ask ι Do not have Do not have 71.6％
At3g61160 Arabidopis thaliana Ask β Do not have Do not have 61.3％
The example that falls into the polypeptide of " shaggy sample kinases or its homologue " definition comprises following sequence: from the shaggy sample kinases γ SEQ ID NO:147 (NCBI accession number AB059621) of rice; Shaggy sample kinases SEQ ID NO:149 (NCBI accession number AK058276) from rice; Shaggy sample kinases SEQ ID NO:151 (NCBI accession number AK099599) from rice; Shaggy sample kinases α SEQ ID NO:153 (NCBI accession number At5g26750) from Arabidopis thaliana; Shaggy sample kinases γ SEQ ID NO:155 (NCBI accession number At3g05840) from Arabidopis thaliana; Shaggy sample kinases α SEQ ID NO:157 (NCBI accession number CAA48538.1) from Arabidopis thaliana; Shaggy sample kinases ε SEQ ID NO:159 (NCBI accession number At5g14640) from Arabidopis thaliana; Shaggy sample kinases γ SEQ ID NO:161 (NCBI accession number CAA73247.1) from Arabidopis thaliana; From zeistic SEQ ID NO:163 (NCBI accession number AY103545); From zeistic SEQ ID NO:165 (NCBI accession number AY108486.1); SEQ ID NO:167 (NCBI accession number CAA48472.1) from clover; SEQ ID NO:169 (NCBI accession number CAA48474.1) from clover; SEQ ID NO:171 (NCBI accession number CAA48473.1) from clover; SEQ ID NO:173 (NCBI accession number CAA54803.1) from tobacco; Infer thing SEQ ID NO:175 (NCBI accession number AAM77397.1) from the kinase whose protein of the shaggy sample of wheat; SEQ ID NO:177 (NCBI accession number CAA58594.1) from petunia.
Term " shaggy sample kinases or its homologue " is not limited to the sequence shown in the SEQ IDNO that aforementioned paragraphs mentions, but any polypeptide that satisfies following standard: it has the sequence identity of (i) and the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/QE/G LK G/N and motif II:K Q/N CXXX G/A/S, wherein X can be arbitrary amino acid, all is applicable to the method for the present invention of implementing.
Shaggy sample kinases as its name hint, have kinase activity." biological chemistry " magazine (The Biochemical Journal, volume 303 (Pt3), on November 1st, 1994,701-704 page or leaf (Stambolic and Woodgett)) has been reported the mensuration of relevant its animal homologue glycogen synthase kinase-3 (GSK-3).
The sequence that can be used for the inventive method is not limited to aforementioned I class homeodomain leucine zipper (HDZip) hox5 nucleic acid and polypeptide; Or aforementioned NRT polypeptide and coding nucleic acid thereof; Or aforementioned YEP16 polypeptide and coding nucleic acid thereof; Or aforementioned I type Shaggy sample kinases and coded polypeptide thereof.The method according to this invention also can be utilized the variant of I class homeodomain leucine zipper (HDZip) hox5 polypeptide or its homologue coding nucleic acid; Or utilize the variant of NRT polypeptide or its homologue coding nucleic acid; Or utilize the YEP16 polypeptide or and the variant of homologue coding nucleic acid; Or utilize the variant of I type Shaggy sample kinases and homologue coding nucleic acid thereof to implement.
The example of this class variant comprises " part ", hybridization sequences, allele variant, splice variant and the variant that obtains by gene reorganization.
For example, can be by nucleic acid being carried out a place or many places lack to prepare " part "." part " can be used with the form of separating, and (or the non-coding) sequence of perhaps can encoding with other merges, so that for example, produces the protein that has made up some activity.When merging with other encoding sequences, the polypeptide that produces after translating may be bigger than " part " of prediction.
Be under the situation of I class HDZip hox5 in the sequence that is used for the inventive method, " part " encoded polypeptides comprises to C-terminal from the N-end: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5.Preferably " part " is the part of one of nucleic acid shown in the Table A.Most preferably " part " is the part of nucleic acid shown in the SEQ ID NO:1.
Be under the situation of NRT coding nucleic acid in the sequence that is used for the inventive method, " part " refer to encode dna fragmentation of such polypeptide, described polypeptide comprises MFS 1 structural domain and is positioned at the membrane spaning domain of described MFS 1 domain C-end.Preferably " part " comprises one or more sequence label defined above.Preferably " part " is the part of one of nucleic acid shown in the Table I.Most preferably " part " is the part of nucleic acid shown in the SEQ ID NO:52.
Be under the situation of YEP16 coding nucleic acid in the sequence that is used for the inventive method, " part " refer to encode dna fragmentation of YEP16 polypeptide or its homologue, according to the preferred sequence that increases progressively, has at least 100 of nucleotide sequence shown in SEQID NO:127 or the SEQ ID NO:129,125,150,175,200,225,250,275,300,325,350,375,400,425,450,475,500,525,550,575 continuous nucleotides, perhaps wherein " part " has at least 100 of YEP16 polypeptide or its homologue nucleic acid sequence encoding according to the preferred sequence that increases progressively, 125,150,175,200,225,250,275,300,325,350,375,400,425,450,475,500,525,550,575 continuous nucleotides.Preferably " part " is the part of nucleic acid shown in SEQ ID NO:127 or the SEQ IDNO:129.
Be under the kinase whose situation of I type Shaggy sample in the sequence that is used for the inventive method, " part " the kinase whose length of Shaggy sample that refers to encode is at least 1, the dna fragmentation of 200 Nucleotide, and described " part " encoded polypeptides has: (i) with the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:KQ/N CXXX G/A/S, wherein X can be arbitrary amino acid.Preferably " part " be arbitrary among SEQ ID NO:146, SEQ ID NO:148, SEQ ID NO:150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:158, SEQ ID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ ID NO:166, SEQ ID NO:168, SEQ ID NO:170, SEQ ID NO:172, SEQ ID NO:174 and the SEQ ID NO:176 shown in the part of nucleic acid.
Therefore the present invention provides the method for improvement plant growth characteristics, comprises the expression of the part of the nucleic acid of regulating encode in the plant I class homeodomain leucine zipper (HDZip) hox5 polypeptide or its homologue; Or comprise the expression of the part of the nucleic acid of regulating coding NRT polypeptide in the plant or its homologue; Or comprise the expression of the part of the nucleic acid of regulating coding YEP16 polypeptide in the plant or its homologue; Or comprise the expression of the part of the nucleic acid of regulating coding I type shaggy sample kinases in the plant or its homologue.
Another kind of nucleic acid variant is such nucleic acid, its can the stringent condition that reduces, preferably under stringent condition with I class HDZip hox5 nucleic acid/gene or its homologue coding nucleic acid; Or with NRT polypeptide or its homologue coding nucleic acid; Or with YEP16 polypeptide or its homologue coding nucleic acid; Or with the hybridization of I type shaggy sample kinases or its homologue coding nucleic acid.
The term " hybridization " of this paper definition refers to the process that the nucleotide sequence of wherein basic homology complementation is annealed each other.Crossover process can take place in solution fully, and namely Hu Bu nucleic acid is all in solution.Crossover process also can so be carried out, and namely one of complementary nucleic acid is fixed on the matrix, on magnetic bead, sepharose 4B or any other resin.In addition, crossover process also can so be carried out, namely wherein one of complementary nucleic acid is fixed on solid support such as nitrocellulose or the nylon membrane, and perhaps for example photolithograph for example is fixed on the siliceous glass support (latter is called nucleic acid array or microarray, or is called nucleic acid chip).For hybridization is taken place, make nucleic acid molecule thermally denature or chemical modification usually, so that two strands is unwind into two strands, and/or remove hairpin structure or other secondary structure in the single-chain nucleic acid.The severity of hybridization is subjected to such as condition effect such as temperature, salt concn, ionic strength and hybridization buffer compositions.
In the nucleic acid hybridization experiment, under the situation as DNA and RNA hybridization (Southern and Northern hybridization), " stringent hybridization condition " and " strict hybridization wash conditions " depends on sequence, and different under different environmental parameters.Those skilled in the art know the multiple parameter that can change in hybridization and washing process, thereby keep or the change stringent condition.
T mBe under the ionic strength of determining and pH value, the temperature of 50% target sequence and the probe hybridization that mates fully.T mThe based composition and the length that depend on solution condition and probe.For example, than long sequence specific hybrid under comparatively high temps.Be lower than T mBe worth 16 ℃ to 32 ℃ and obtain maximum hybrid rate.In hybridization solution, exist monovalent cation can reduce Coulomb repulsion effect between two nucleic acid chains, thereby promote heterozygote to form; When na concn during up to 0.4M, this effect is obvious.The methane amide of each percentage point can make the melting temperature(Tm) of DNA-DNA and DNA-RNA duplex reduce by 0.6 to 0.7 ℃, add 50% methane amide hybridization is finished at 30 to 45 ℃, but this will reduce hybrid rate.Base-pair mismatch reduces the thermostability of hybrid rate and duplex.On average, for big probe, each percentage point base mispairing makes T mValue descends about 1 ℃.T mValue can be calculated with the following equation that depends on the heterozygote type:
1.DNA-DNA heterozygote (Meinkoth and Wahl, Anal.Biochem., 138:267-284,1984):
Tm=81.5 ℃+16.6xlog 10[Na +] a+ 0.41x%[G/C b]-500x[L c] -1-0.61 * % methane amide
2.DNA-RNA or RNA-RNA heterozygote:
Tm＝79.8+18.5(log 10[Na +] a)+0.58(％G/C b)+11.8(％G/C b) 2-820/L c
3. few DNA or few RNA dHeterozygote:
＜20 Nucleotide: Tm=2 (l n)
20-35 Nucleotide: Tm=22+1.46 (l n)
aOr it is for other monovalent cation, but only accurate in the 0.01-0.4M scope.
bOnly accurate for the %GC of scope 30% to 75%.
cThe base pair length of L=duplex.
dThe widow, oligonucleotide; l n, the useful length of primer=2 * (G/C number)+(A/T number).
Note is for per 1% methane amide, T mValue reduces about 0.6 to 0.7 ℃, and the existence of 6M urea can make T mValue reduces about 30 ℃.
The hybridization specificity is the function of post-hybridization washing normally.In order to remove the background that non-specific hybridization produces, with the salts solution washing sample of dilution.The key factor of this class washing comprises ionic strength and the temperature of final washing soln: salt concn is more low, wash temperature is more high, and the severity of washing is just more high.Wash conditions is carried out under the stringency being equal to or less than hybridization usually.Usually, the stringent condition that is applicable to that nucleic acid hybridization is measured or the gene amplification detection is operated as above is set.Also can select higher or lower stringency.Usually, at the ionic strength of determining and the particular sequence under the pH value, select specific heat melting temperature(Tm) (T m) low about 50 ℃ low stringency condition.Medium stringent condition is that temperature compares T mLow 20 ℃, high stringent condition is that temperature compares T mLow 10 ℃.For example, stringent condition is strict as for example condition A-L at least; The stringent condition that reduces is strict as for example condition M-R at least.Can be by the arbitrary non-specific binding of controlling in many known technologies, described technology such as, for example use proteinaceous solution closing membrane, in hybridization buffer, add allos RNA, DNA and SDS, and handle with the RNA enzyme.Following table 2 has been listed the example of hybridization and wash conditions.
Table 2: the example of hybridization and wash conditions
Figure S200680049144XD00291
Figure S200680049144XD00301
Figure S200680049144XD00302
" hybrid length " is the expection length of hybrid nucleic acid.When the nucleic acid hybridization of known array, can and differentiate that conservative region as herein described determines hybrid length by aligned sequences.
In hybridization and lavation buffer solution, can (1 * SSPE be 0.15M NaCl, 10mM NaH with SSPE 2PO4 and 1.25mM EDTA pH7.4) replace SSC (1 * SSC is 0.15MNaCl and 15mM Trisodium Citrate); Washing was 15 minutes after hybridization was finished.Hybridization and washing can additionally comprise 5 * Denhardt ' s reagent, 0.5-1.0%SDS, 100 μ g/ml sex change fragmentation salmon sperm DNA, 0.5% trisodium phosphate and up to 50% methane amide.
*Tb-Tr: for the heterozygote of expection length less than 50 base pairs, hybridization temperature should be than the melting temperature(Tm) T of heterozygote mLow 5-10 ℃; Determine T according to aforesaid equation m
±The present invention comprises that also the nucleic acid with PNA or modification replaces arbitrary or a plurality of DNA or RNA hybridization mating partner.
In order to define the severity level, can be with reference to (2001) such as Sambrook " molecular cloning: laboratory manual " the 3rd edition, cold spring harbor laboratory publishes, the cold spring port, New York, perhaps CurrentProtocols in Molecular Biology, John Wiley ﹠amp; Sons, N.Y. (1989).
It is that the hybridization sequences encoded polypeptides comprises to the C-end from the N-end under the situation of I class HDZip hox5 in the sequence that is used for the inventive method: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5.Preferred hybridization sequences can the stringent condition that reduces, preferably under stringent condition with one of nucleic acid shown in the Table A or its part hybridization as hereinbefore defined.Most preferably described part is the part of the nucleic acid shown in the SEQ ID NO:1.
Be under the situation of NRT polypeptide or its homologue coding nucleic acid in the sequence that is used for the inventive method, hybridization sequences be can the stringent condition that reduces, preferably under stringent condition with the nucleic acid/gene of NRT nucleic acid/gene recombination, the polypeptide of described NRT nucleic acid/genes encoding comprises the MFS_1 structural domain and is positioned at the membrane spaning domain of described MFS_1 domain C-end, and preferably also comprises one or more sequence label defined above.Preferred hybridization sequences can with nucleic acid shown in embodiment 14 Table I or with the part hybridization of arbitrary nucleic acid shown in the Table I, wherein " part " as hereinbefore defined.Most preferably hybridization sequences can be hybridized with SEQ ID NO:52.
Be under the situation of YEP16 polypeptide or its homologue coding nucleic acid in the sequence that is used for the inventive method, hybridization sequences is such nucleotide sequence, its can the stringent condition that reduces, preferably under stringent condition with the nucleic acid array hybridizing of coding YEP16 polypeptide or its homologue.Preferred hybridization sequences can be under the stringent condition that reduces and the nucleic acid hybridization shown in SEQ ID NO:127 or the SEQ ID NO:129.
Be under the situation of I type shaggy sample kinases coding nucleic acid at the nucleic acid that is used for the inventive method, hybridization sequences is such nucleic acid, its can the stringent condition that reduces, preferably under stringent condition with I type shaggy sample kinases coding nucleic acid/gene recombination defined above, described hybridization sequences encoded polypeptides has: (i) with the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/N CXXX G/A/S, wherein X can be arbitrary amino acid.Hybridization sequences length is at least 1,200 Nucleotide.Preferred hybridization sequences can with the nucleic acid hybridization shown in arbitrary among SEQ ID NO:146, SEQ ID NO:148, SEQ ID NO:150, SEQID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:158, SEQID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ ID NO:166, SEQID NO:168, SEQ ID NO:170, SEQ ID NO:172, SEQ ID NO:174 and the SEQID NO:176.
Therefore the present invention provides the method for improvement plant growth characteristics, comprise regulate in the plant can the stringent condition that reduces, preferably under stringent condition with the expression of nucleic acids of I class homeodomain leucine zipper (HDZip) hox5 polypeptide or the hybridization of its homologue coding nucleic acid; Or comprise regulate in the plant can the stringent condition that reduces, preferably under stringent condition with the expression of nucleic acids of NRT polypeptide or the hybridization of its homologue coding nucleic acid; Or comprise regulate in the plant can the stringent condition that reduces, preferably under stringent condition with the expression of nucleic acids of YEP16 polypeptide or the hybridization of its homologue coding nucleic acid; Or comprise regulate in the plant can the stringent condition that reduces, preferably under stringent condition with the expression of nucleic acids of I type shaggy sample kinases or the hybridization of its homologue coding nucleic acid.
Nucleic acid or its variant can be from any natural or artificial sources.Nucleic acid/gene or its variant can separate from microbe-derived as yeast or fungi, or separate and originate from plant, algae or animal (comprising the mankind).Can be by careful manual operation at the natural form of modifying described nucleic acid aspect composition and/or the genome environment.No matter the nucleic acid in preferred plant source derives from same plant species (for example for its species to be introduced) or derives from different plant species.Can preferably from Gramineae (Poaceae), more preferably from Oryza, most preferably from rice, separate described nucleic acid from the unifacial leaf species.Can preferably from Cruciferae (Brassicaceae), more preferably separate described nucleic acid from Arabidopis thaliana from dicotyledonous species.
Can regulate expression of nucleic acids by introducing genetic modification (the preferably locus of discussing).Locus defined herein means the genome district, and it comprises the 10kb in goal gene and upstream of coding region or downstream.
For example, can introduce genetic modification by following any (or multiple) method: T-DNA activation, TILLING, site-directed mutagenesis, orthogenesis and homologous recombination, or by in plant, introducing and express I class HDZip hox5 polypeptide or its homologue coding nucleic acid; Or by in plant, introducing and express NRT or its homologue coding nucleic acid; Or by in plant, introducing and express YEP16 or its homologue coding nucleic acid; Or by in plant, introducing and express I type shaggy sample kinases or its homologue coding nucleic acid.Introducing genetic modification step afterwards is the nucleic acid of selecting to express through regulating, and described expression is regulated and made plant have the plant growth characteristics, the particularly productive rate of Zeng Jiaing of improvement.
T-DNA activation tagging (Science (1992) 1350-1353 such as Hayashi) comprises that the T-DNA that will contain promotor (also can be translational enhancer or intron) usually is inserted in genome district or gene coding region upstream or the downstream 10kb of goal gene, thereby makes promotor can instruct the expression of target gene in configuration.Usually natural promoter is destroyed to the regulation and control of target gene expression, and gene is controlled by the new promotor of introducing.Promotor generally is contained among the T-DNA.This T-DNA inserts Plant Genome at random, for example, infects insertion by Agrobacterium (Agrobacterium), and causes near the gene overexpression insertion T-DNA.The transgenic plant that obtain are owing near the gene overexpression promotor of introducing shows the dominant phenotype.Promotor to be introduced can be can [being plant] instruct the promotor of genetic expression in this case in the expectation organism arbitrarily.For example, composing type, that organize the preference type, the cell type preference is type is applicable to all that with promotor induction type T-DNA activates.
Also can genetic modification be introduced the locus of discussing by TILLING (the local sudden change of the genome of targeted induction) technology.This is a kind of induced-mutation technique, for generation of and/or identify and separate the variant of mutagenesis.TILLING also allows to select to carry the plant of this type of mutation variants.These mutation variants even may show the higher activity that the gene than natural form shows.TILLING combines high-density mutagenesis and high-throughput screening method.The step that TILLING generally follows is: (a) EMS mutagenesis (Redei GP and Koncz C (1992) In Methods in Arabidopsis Research, Koncz C, Chua NH, Schell J writes Singapore, World Scientific PublishingCo, the 16-82 page or leaf; Feldmann etc. (1994) In Meyerowitz EM, Somerville CR writes, Arabidopsis.Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 137-172 page or leaf; Lightner J and Caspar T (1998) In J Martinez-Zapater, JSalinas writes, Methods on Molecular Biology rolls up 82 Humana Press, Totowa, NJ, 91-104 page or leaf); (b) DNA preparation and individual the merging; (c) pcr amplification in purpose zone; (d) sex change and annealing are to form assorted duplex; (e) DHPLC, the assorted duplex that wherein exists in the storehouse can detect extra peak at color atlas; (f) evaluation of mutated individual; (g) order-checking of sudden change PCR product.The method of TILLING is that well-known in the art (McCallum etc. (2000) NatBiotechnol 18:455-7 is by Stemple summary (2004) Nat Rev Genet 5 (2): 145-50).
Site-directed mutagenesis can be used for producing variant nucleic acid.Several different methods can be used to realize site-directed mutagenesis, and (Current Protocols in Molecular Biology.Wiley writes the most frequently used method that is based on PCR, www.4ulr.com/products/currentprotocols/index.html).
Orthogenesis gene reorganization in other words also can be used for producing the nucleic acid variant.This comprises the repetition of DNA reorganization, is suitable screening and/or selection subsequently, has bioactive variant (Castle etc., (2004) Science 304 (5674): 1151-4 of modification with generation; United States Patent (USP) 5,811,238 and 6,395,547).
TDNA activation, TILLING, site-directed mutagenesis and orthogenesis are the examples that can produce the technology of new allelotrope and variant.
Homologous recombination can be introduced selected nucleic acid to the appointment selected location in the genome.Homologous recombination is the conventional standard technique of using in the bio-science, and it is used for unicellular lower eukaryote body such as yeast or small liwan moss.(Offringa etc. (1990) EMBO is (10) J.9: 3077-84), and describe (Terada etc. (2002) Nat Biotech 20 (10): 1030-4 in crop plants such as rice for the not only description in model plant of the method for enforcement homologous recombination in plant; Iida and Terada (2004) Curr Opin Biotech15 (2): 132-8).The nucleic acid of institute's target need not the locus that target is discussed, but can be incorporated into for example high expression level zone.The nucleic acid of institute's target can be the allelotrope of improvement, and it is used for replacing native gene or additionally introduces except native gene again.
The preferred method of introducing genetic modification is to introduce and express I class HDZip hox5 polypeptide or its homologue coding nucleic acid in plant; Or in plant, introduce and expression NRT or its homologue coding nucleic acid; Or in plant, introduce and expression YEP16 or its homologue coding nucleic acid; Or in plant, introduce and expression I type shaggy sample kinases or its homologue coding nucleic acid.The nucleic acid of introduced plant can be nucleic acid or " part " defined above or the hybridization sequences of total length.
Be under the situation of YEP16 polypeptide or its homologue in the sequence that is used for the inventive method, preferred target plastid; Method with protein target plastid is well-known in the art like this, just as using transit peptides to carry out this type of target.Following table 3 has shown the example that is suitable for the transit peptides of any YEP16 polypeptide or its homologue target plastid, preferred chloroplast(id).Preferably use natural transit peptides for the YEP16 peptide sequence of SEQ ID NO:128, its natural transit peptides is shown in SEQ ID NO:131.SEQ ID NO:130 represents the YEP16 polypeptide chain and is used for the natural transit peptides (nucleotide sequence of the aminoacid sequence of SEQ ID NO:129 representative coding SEQ ID NO:130) of target chloroplast(id) with it.Most preferably use the natural transit peptides shown in the SEQ ID NO:131 to come the YEP16 polypeptide shown in the target SEQ ID NO:128, but any transit peptides all can use with YEP16 polypeptide or its homologue.
Table 3: be used for the example with the transit peptide sequence of amino acid target plastid
NCBI accession number/SEQ ID NO The source is biological Protein function Transit peptide sequence
SEQ ID NO：131 MDTLSASVSSLNLPSLPPPPQPPLRSISSRF KSTVNATTSASSTNLSKPTSSSPSSS
SEQ ID NO：132- P07839 Chlamydomonas (Chlamydomonas) Ferredoxin MAMAMRSTFAARVGAKPAVRGARPASR MSCMA
SEQ ID NO：133- AR23425 Chlamydomonas The Rubisco activating enzymes MQVTMKSSAVSGQRVGGARVATRSVRR AQLQV
SEQ ID NO：134- AA56932 Arabidopis thaliana The asp transaminase MASLMLSLGSTSLLPREINKDKLKLGTSA SNPFLKAKSFSRVTMTVAVKPSR
SEQ ID NO：135- CAA31991 Arabidopis thaliana Acyl carrier protein 1 MATQFSASVSLQTSCLATTRISFQKPALIS NHGKTNLSFNLRRSIPSRRLSVSC
SEQ IDNO：136- CAB63798 Arabidopis thaliana Acyl carrier protein 2 MASIAASASISLQARPRQLAIAASQVKSF SNGRRSSLSFNLRQLPTRLTVSCAAKPET VDKVCAVVRKQL
SEQ IDNO：137- CAB63799 Arabidopis thaliana Acyl carrier protein 3 MASIATSASTSLQARPRQLVIGAKQVKSF SYGSRSNLSFNLRQLPTRLTVYCAAKPET VDKVCAVVRKQLSLKE
SEQ ID NO： 138-NP_199191 Arabidopis thaliana The ATP sulfurylase MASSAAAIVSGSPFRSSPLIHNHHASRYA PGSISVVSLPRQVSRRGLSVKS
SEQ IDNO：139- CAA81736 Colea RuBisCO subunit bindin alpha subunit MATANALSSPSVLCSSRQGKLSGGSQQK GQRVSYRKANRRFSLRANVKEIAFDQSS
RAALQAGIDKLADAVGLTLGPRGRNVV LDEFGSPKVVNDGVTIA
SEQ IDNO：140- AAA87039 Barley Ribulose-1,5-bisphosphate, 5-bisphosphate carboxylase small subunit MAPTVMASSATSVAPFQGLKSTAGLPVS RRSNAS SASVSNGGRIRCMQVWPIEGIK KFETLSYLPPLSTEALLKQVDYLIRSKWV PCLEFSKVGFIFREH
SEQ ID NO：141- AAC82334 Puncture vine clover (Medicago truncatula) Gamma-glutamylcysteine synthetase MTTIFRLASSSSPSLRHDATPHNFHIRKTS ISNTFSFSSKNSLSFKRILTSGGSRRFIVAA SPPTEDAVVATEPLTKQDLIDYLASGCKT KDKWRIGTEH
SEQ IDNO：142- AAB81104 Spinach (Spinacia oleracea) Sedoheptulose-1, the 7-diphosphatase METSMACCSRSIVLPRVSPQHSSALVPSSI NLKSLKSSSLFGESLRMTTKSSVRVNKA KNSSLVTKCELGDSLEEFLAKATTDKGLI RLMMCMGEALRTI
" homologue " of protein comprises peptide, oligopeptides, polypeptide, protein and enzyme, it has aminoacid replacement, disappearance and/or insertion with respect to the unmodified protein matter of discussing, and to its derived from unmodified protein matter have similar biological activity and functionally active.For producing such homologue, the amino acid of protein can be replaced by other amino acid with similar characteristics (as similar hydrophobicity, wetting ability, antigenicity, form or break the tendency of αLuo Xuanjiegou or β laminated structure).Conservative replacement table is (for example seeing Creighton (1984) Proteins.W.H.Freemanand Company and following table 4) well-known in the art.Be used for being preferably such polypeptide according to the homologue of the inventive method, its sequence that can be used for the inventive method of mentioning according to the preferred sequence that increases progressively and this paper [for example sequence shown in this paper Table A and the I] has at least 30%, 40%, 50%, 60%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or higher sequence identity or similarity (function identity).
Term " homologue " also comprises the homologue of two kinds of special shapes, and this comprises lineal homologous sequence and collateral line homologous sequence, and it is contained for the evolution concept of describing the gene ancestral relationship.Term " collateral line homologue " is relevant with the gene replication in producing the homogenic species gene group of collateral line.It is relevant to come from the homologous gene that species form in term " lineal homologue " and the different organisms.
For example, can search for the lineal homologue that easily finds in the monocotyledons species by carrying out so-called mutual blast.Lineal homologue and collateral line homologue with searching I class HDZip hox5 nucleic acid or I class HDZip hox5 polypeptide are example, this can realize by a BLAST, comprise sequence (for example SEQ ID NO:1 or SEQ ID NO:2) that use discusses at any sequence library as can public acquisition see Http:// www.ncbi.nlm.nih.govNcbi database carry out BLAST.When beginning from nucleotide sequence, can use BLASTN or TBLASTX, utilize standard default, and when beginning from protein sequence, can use BLASTP or TBLASTN, utilize standard default.BLAST result can randomly filter.Then use the full length sequence among filtering result or the unfiltered result to carry out reverse BLAST (quadratic B LAST) at the sequence of the sequence source of discussing organism certainly.The result of BLAST and quadratic B LAST relatively then.If the result of quadratic B LAST as the hit event of high similarity, has then found the collateral line homologue with I class HDZip hox5 nucleic acid or I class HDZip hox5 polypeptide, if its with BLAST in used sequence from identical biology.From the biology that is different from used sequence among BLAST, then found lineal homologue at it.Under the situation of extended familys, can use ClustalW, then set up contiguous threaded tree, assist cluster visual.
Also can utilize identical method to seek lineal homologue and the collateral line homologue of NRT coding nucleic acid and NRT polypeptide; Seek lineal homologue and the collateral line homologue of YEP16 coding nucleic acid and YEP16 polypeptide; And lineal homologue and the collateral line homologue of seeking I type shaggy sample kinases coding nucleic acid and I type shaggy sample kinase polypeptide.
Homologue can be the form of protein " replacement variant ", namely has at least a residue to be removed in aminoacid sequence, and inserts different residues in its position.Aminoacid replacement is the replacement of single residue normally, and still deciding on the functional limitations that puts on polypeptide also can be that cluster replaces; Insert the common order of magnitude at about 1 to 10 amino-acid residue.Preferred amino acid replaces and comprises conservative aminoacid replacement.Conservative replacement table is the many acquisitions easily in this area.Following table has provided the example that conserved amino acid replaces.
Table 4: the example that conserved amino acid replaces
Residue The conservative replacement Residue The conservative replacement
Ala Ser Leu Ile；Val
Arg Lys Lys Arg；Gln
Asn Gln；His Met Leu；Ile
Asp Glu Phe Met；Leu；Tyr
Gln Asn Ser Thr；Gly
Cys Ser Thr Ser；Val
Glu Asp Trp Tyr
Gly Pro Tr Trp；Phe
His Asn；Gln Val Ile；Leu
Ile Leu；Val
Homologue also can be the form of protein " insertion variant ", namely introduces one or more amino-acid residues in the predetermined position of protein.Insertion can comprise the fusion of N-end and/or C-end, and single or multiple amino acid whose internal sequence inserts.Usually, the insertion of aminoacid sequence inside will be less than the fusion of N-or C-end, and the order of magnitude is about 1 to 10 residue.The example of the terminal fused protein of N-or C-or peptide be included in the binding domains of the activating transcription factor of using in the yeast two-hybrid system or activation structure territory, bacteriophage coat protein matter, (Histidine) 6 labels, glutathione S-transferase label, a-protein, maltose binding protein, Tetrahydrofolate dehydrogenase, Tag100 epi-position, c-myc epi-position,
Figure S200680049144XD00381
Epi-position, lacZ, CMP (calmodulin binding peptide), HA epi-position, protein C epi-position and VSV epi-position.
The homologue of protein " disappearance variant " form is characterised in that removes one or more amino acid from protein.
Can be by peptide synthetic technology well-known in the art, as solid phase method of peptide synthesis etc., or by the recombinant DNA processing ease obtain the amino acid variant of protein.Dna sequence dna working method for generation of the replacement of protein, insertion or disappearance variant is well-known in the art.For example, those skilled in the art know the technology that produces the replacement sudden change in the DNA predetermined position, it comprises M13 mutagenesis, T7-Gen vitro mutagenesis (USB, Cleveland, OH), QuickChange site-directed mutagenesis (Stratagene, San Diego, CA), site-directed mutagenesis or other site-directed mutagenesis method of PCR mediation.
I class HDZip hox5 polypeptide or its homologue; Nitrate transport protein (NRT) or its homologue coding nucleic acid; YEP16 peptide coding nucleic acid; And I type shaggy sample kinases or its homologue coding nucleic acid can be derivatives." derivative " comprises peptide, oligopeptides, polypeptide, protein and enzyme, compares with the aminoacid sequence of natural generation formal protein, and it can comprise replacement, disappearance or the natural and amino-acid residue non-natural generation that adds.Compare with natural generation form amino acid sequence of polypeptide, derivative can comprise amino-acid residue naturally occurring change, glycosylated, acylations, prenylation or that non-natural produces.Derivative can also comprise the one or more non-aminoacid replacement with respect to its aminoacid sequence that is derived from, for example be incorporated into reporter molecules or other part of aminoacid sequence covalently or non-covalently, for example be combined with it and be beneficial to the reporter molecules that derivative detects, and the amino-acid residue that non-natural produces for the aminoacid sequence of natural generation protein.
I class HDZip hox5 polypeptide or its homologue can be encoded by the alternative splicing variant; NRT polypeptide or its homologue can be encoded by the alternative splicing variant; YEP16 polypeptide or its homologue can be encoded by the alternative splicing variant; And I type shaggy sample kinases or its homologue can be encoded by the alternative splicing variant.
It is that the splice variant encoded polypeptides comprises to the C-end from the N-end under the situation of I class HDZip hox5 polypeptide or its homologue coding nucleic acid in the sequence that is used for the inventive method: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5.In addition, I class HDZip hox5 polypeptide or its homologue can comprise one of following or both have both at the same time: (a) Trp tail; (b) RPFF amino acid motif, wherein R is Arg, P is Pro, and F is Phe.In this motif, allow to carry out the one or more conservative change on the optional position, and/or one or two the non-conservative change on the optional position.When end was studied protein from the N-end to C-, (b) motif in was positioned at before the acid box.The splice variant of nucleotide sequence shown in the preferred Table A also.The splice variant of nucleotide sequence shown in the SEQID NO:1 most preferably.
Be under the situation of NRT polypeptide or its homologue or its coding nucleic acid in the sequence that is used for the inventive method, the splice variant encoded polypeptides comprises the MFS_1 structural domain and is positioned at the membrane spaning domain of described MFS_1 domain C-end, and preferably also comprises the conservative sequence label of one or more SEQ ID NO:57 to 64 defined above.The splice variant of arbitrary nucleic acid shown in the preferred Table I also.The splice variant of the nucleic acid of SEQ ID NO:52 most preferably.
Be under the situation of YEP16 coding nucleic acid in the sequence that is used for the inventive method, splice variant is the splice variant of nucleotide sequence shown in SEQ ID NO:127 or the SEQ ID NO:129.
Be under the situation of I type shaggy sample kinases or its homologue coding nucleic acid in the sequence that is used for the inventive method, the alternative splicing variant is SEQ ID NO:146, SEQ ID NO:148, SEQ IDNO:150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ IDNO:158, SEQ ID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ IDNO:166, SEQ ID NO:168, SEQ ID NO:170, SEQ ID NO:172, the splice variant of the nucleic acid shown in arbitrary among SEQ IDNO:174 and the SEQ ID NO:176.Also preferred splice variant encoded polypeptides has: (i) with the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/NCXXX G/A/S, wherein X can be arbitrary amino acid.
Therefore the present invention provides the method for improvement plant growth characteristics, comprises the expression of the alternative splicing variant of regulating I class homeodomain leucine zipper (HDZip) hox5 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the alternative splicing variant of regulating NRT polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the alternative splicing variant of regulating YEP16 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the alternative splicing variant of regulating I type glycogen synthase kinase (I type shaggy sample kinases) in the plant or its homologue coding nucleic acid.
Term used herein " alternative splicing variant " comprises such nucleotide sequence variant, wherein the intron of Xuan Zeing and/or exon cut, replace or add or wherein intron be shortened or lengthen.Such variant will be the bioactive variant of retaining protein, and this can realize by the functional fragment of retaining protein optionally.Such splice variant can be natural or artificial.The method that produces this class splice variant is well-known in the art.
Homologue can be by the allele variant coding of I class HDZip hox5 polypeptide or its homologue coding nucleic acid; Or by the allele variant of nitrate transport protein (NRT) or its homologue coding nucleic acid coding; Or by the allele variant of YEP16 peptide coding nucleic acid coding; Or by the allele variant of I type shaggy sample kinases or its homologue coding nucleic acid coding.
It is that the allele variant encoded polypeptides comprises to the C-end from the N-end under the situation of I class HDZip hox5 polypeptide or its homologue coding nucleic acid in the sequence that is used for the inventive method: (i) acid box; (ii) I class homeodomain; The leucine zipper that (iii) has seven peptides more than 5.In addition, I class HDZip hox5 polypeptide or its homologue can comprise one of following or both have both at the same time: (a) Trp tail; (b) RPFF amino acid motif, wherein R is Arg, P is Pro, and F is Phe.In this motif, allow to carry out the one or more conservative change on the optional position, and/or one or two the non-conservative change on the optional position.When end was studied protein from the N-end to C-, (b) motif in was positioned at before the acid box.The allele variant of nucleotide sequence shown in the preferred Table A also.The allele variant of nucleotide sequence shown in the SEQ ID NO:1 most preferably.
Be under the situation of NRT polypeptide or its homologue or its coding nucleic acid in the sequence that is used for the inventive method, the allele variant encoded polypeptides comprises the MFS_1 structural domain and is positioned at the membrane spaning domain of described MFS_1 domain C-end, and preferably also comprises the conservative sequence label of one or more SEQ IDNO:57 to 64 defined above.The allele variant of arbitrary nucleic acid shown in the preferred Table I also.The allele variant of the nucleic acid of SEQ ID NO:52 most preferably.
Be under the situation of YEP16 coding nucleic acid in the sequence that is used for the inventive method, allele variant is the allele variant of nucleotide sequence shown in SEQ ID NO:127 or the SEQ ID NO:129.
Be under the situation of I type shaggy sample kinases or its homologue coding nucleic acid in the sequence that is used for the inventive method, allele variant is SEQ ID NO:146, SEQ ID NO:148, SEQ ID NO:150, SEQ ID NO:152, SEQ ID NO:154, SEQ ID NO:156, SEQ ID NO:158, SEQ ID NO:160, SEQ ID NO:162, SEQ ID NO:164, SEQ ID NO:166, SEQ ID NO:168, SEQ ID NO:170, SEQ ID NO:172, the allele variant of the nucleic acid shown in arbitrary among SEQ ID NO:174 and the SEQ ID NO:176.Also preferred allele variant encoded polypeptides has: (i) with the sequence identity of the aminoacid sequence at least 77% shown in the SEQ ID NO:147; And (ii) motif I:R/H/V/N/Q E/G LK G/N and motif II:K Q/NCXXX G/A/S, wherein X can be arbitrary amino acid.
Therefore the present invention provides the method for improvement plant growth characteristics, comprises the expression of the allele variant of regulating I class homeodomain leucine zipper (HDZip) hox5 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the allele variant of regulating NRT polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the allele variant of regulating YEP16 polypeptide in the plant or its homologue coding nucleic acid; Or comprise the expression of the allele variant of regulating I type glycogen synthase kinase (I type shaggy sample kinases) in the plant or its homologue coding nucleic acid.
The natural existence of allele variant, and comprise in the methods of the invention these natural allelic purposes that has.Allele variant comprises single nucleotide polymorphism (SNP) and small-sized insertion/deletion polymorphism (INDEL).The size of INDEL is usually less than 100bp.SNP and INDEL have formed a maximum class sequence variants in the naturally occurring polymorphism strain of most of biologies.
According to the present invention, consider to regulate the expression of nucleic acid or its variant.The method of regulatory gene or gene product expression has detailed record in this area, and for example comprises the use of crossing expression, transcriptional enhancer or translational enhancer with suitable promoters driven.The nucleic acid that act as the separation of promotor or enhancer element can be introduced on the appropriate location of non-allos form polynucleotide (being generally the upstream), thereby raise the expression of nucleic acid or its variant.For example, can by sudden change, disappearance and/or replace to change in the body endogenesis promoter (referring to Kmiec, United States Patent (USP) 5,565, No. 350; Zarling etc., PCT/US93/03868), perhaps can be with the promotor of separating and gene of the present invention with suitable direction with apart from the introduced plant cell, thus the expression of controlling gene.
The method that reduces gene or gene product expression has detailed record in this area.
If the expectation expression of polypeptides will be included the polyadenylic acid zone at the 3 ' end in polynucleotide encoding district usually.The polyadenylic acid zone can be derived from natural gene, multiple other plant gene or T-DNA.For example, 3 ' of adding terminal sequence can be derived from nopaline synthase or octopine synthase gene or be derived from the other plant gene alternatively or be derived from any other eukaryotic gene.
Also can in the encoding sequence of 5 ' non-translational region or part encoding sequence, add intron sequences, to be increased in the ripe courier's who accumulates in the cytosol amount.Shown that but the montage intron of including in the plant and animal expression construct transcription unit all can make the genetic expression raising reach 1000 times of (Buchman and Berg (1988) Mol.Cell biol.8:4395-4405 at mRNA and protein level; Callis etc. (1987) Genes Dev.1:1183-1200).When usually this class intron was placed near transcription unit's 5 ' end, it improved the effect maximum of genetic expression.Corn intron A dh1- S introne 1,2 and 6 and the use of Bronze-1 intron be well known in the art.Usually see The MaizeHandbook, 116 chapters, Freeling and Walbot write, Springer, N.Y. (1994).
The present invention also provides genetic constructs and carrier, to promote introducing and/or the expression for the nucleotide sequence of the inventive method.
Therefore, the gene construct that provides contains:
(i) I class HDZip hox5 nucleic acid or its variant; Or NRT coding nucleic acid or its variant; Or YEP16 coding nucleic acid or its variant; Or I type shaggy sample kinases coding nucleic acid or its variant;
(ii) one or morely can drive the control sequences that (i) amplifying nucleic acid sequence is expressed; With optional
(iii) transcription termination sequence.
Can use recombinant DNA technology well known to those skilled in the art to make up the construct that is used for the inventive method.Gene construct can be inserted commercially available, be suitable for transforming and enter plant and be suitable in cell transformed expressing in the carrier of goal gene.Therefore the present invention provides the purposes in the methods of the invention of gene construct as hereinbefore defined.
Transform plant with the carrier that contains aim sequence.Aim sequence effectively is connected in one or more control sequences (being connected in promotor at least).Term " controlling element ", " control sequence " and " promotor " all are used interchangeably at this paper, from broadly referring to influence the regulation and control nucleotide sequence of the sequence expression that is attached thereto.Above-mentioned term comprises that being derived from typical eukaryotic gene group gene transcription regulating and controlling sequence (comprises the TATA box that has or do not have CCAAT box sequence, it is essential for accurate transcription initiation), and other controlling element (being upstream activating sequence, enhanser and silencer), it is by replying growth stimulation and/or outside stimulus or changing genetic expression in tissue-specific mode.This term also comprises the transcription regulating nucleotide sequence of classical prokaryotic gene, can comprise-35 box sequences and/or-10 box transcription regulating nucleotide sequences in the case.Term " controlling element " also comprises synthetic fusion molecule or derivative, and it gives, activates or strengthen the expression of cell, tissue or organ amplifying nucleic acid molecule.Term used herein " effectively connect " refers to the functional connection between promoter sequence and the goal gene, thereby promoter sequence can initial goal gene transcribes.
Advantageously, can use the expression of the promoters driven nucleotide sequence of any kind.Promotor can be constitutive promoter, namely replys growth, chemistry, environment or physical stimulation and has the transcription initiation of inducing or increase.The example of inducible promoter is stress induced promoter, i.e. the promotor that activates when the multiple stress conditions of plant contact.In addition or optionally, promotor can be to organize the promotor of preference type, namely can organize at some, as preferential initial promotor of transcribing in tissues such as leaf, root, seed.Can be only in some tissue initial promotor of transcribing be referred to herein as " tissue specificity " promotor.
Preferred I class HDZip hox5 nucleic acid or its variant; NRT coding nucleic acid or its variant; YEP16 coding nucleic acid or its variant; I type shaggy sample kinases coding nucleic acid or its variant effectively are connected in constitutive promoter.Constitutive promoter and is generally expressed basically at great majority but need not all g and D stage transcriptional activations.Constitutive promoter is preferably the GOS2 promotor, more preferably constitutive promoter is rice GOS2 promotor, also preferred constitutive promoter is shown in the nucleotide sequence that is substantially similar to SEQID NO:33 or SEQ ID NO:178, and most preferably constitutive promoter is shown in SEQ ID NO:33 or SEQ ID NO:178.Should clear and definite application of the present invention be not limited to I class HDZip hox5 nucleic acid or its variant by the GOS2 promoters driven; NRT coding nucleic acid or its variant; Or I type shaggy sample kinases coding nucleic acid or its variant.Other example that also can be used for implementing the constitutive promoter of the inventive method is shown in following table 5.
Table 5: the example of constitutive promoter
Gene source Reference
Actin muscle McElroy etc., Plant Cell, 2:163-171,1990
CAMV 35S Odell etc., Nature, 313:810-812,1985
CaMV 19S Nilsson etc., Physiol.Plant.100:456-462,1997
GOS2 De Pater etc., Plant J Nov; 2 (6): 837-44,1992, WO 2004/065596
Ubiquitin Christensen etc., Plant Mol.Biol.18:675-689,1992
The rice cyclophilin Buchholz etc., Plant Mol Biol.25 (5): 837-43,1994
Corn H3 histone Lepetit etc., Mol.Gen.Genet.231:276-285,1992
Clover H3 histone Plant Mol.Biol.11:641-649 such as Wu, 1988
Actin muscle 2 An etc., Plant are (1) J.10; 107-121,1996
34S FMV Sanger et al.，Plant.Mol.Biol.，14，1990：433-443
The Rubisco small subunit US 4,962,028
OCS Leisner(1988)Proc Natl Acad Sci USA 85(5)：2553
SAD1 Jain etc., Crop Science, 39 (6), 1999:1696
SAD2 Jain etc., Crop Science, 39 (6), 1999:1696
Nos Shaw etc. (1984) Nucleic Acids Res.12 (20): 7831-7846
V-ATPase WO 01/14572
Super promotor WO 95/14098
G box protein matter WO 94/12015
The nucleotide sequence of preferred coding YEP16 polypeptide or its homology effectively is connected in seed specific promoters.Seed specific promoters is transcriptional activation in seed tissue mainly.Seed-specific starts also can have certain residual activity in other parts.The preferred seed specificity starts and to be embryo-specific and/or aleuron specificity promoter, i.e. the main transcriptional activation in seed endosperm tissue and/or aleurone layer of described promotor, but may have certain residual activity or leakage expression elsewhere.Preferred promoter is the oleosin promotor, for example from rice (SEQ ID NO:143).Should clear and definite application of the present invention be not limited to the YEP16 coding nucleic acid shown in SEQ ID NO:127 or the SEQ ID NO:129, and application of the present invention also is not limited to the expression by the YEP16 coding nucleic acid of oleosin promoters driven.Other example that also can be used for driving the seed specific promoters that YEP16 polypeptide or its homologue coding nucleic acid express is shown in following table 6.
Table 6: the example of seed specific promoters
Gene source Expression pattern Reference
The seed-specific gene Seed Simon etc., Plant Mol.Biol.5:191,1985; Scofield etc., J.Biol.Chem.262:12202,1987; Baszczynski etc., Plant Mol.Biol.14:633,1990.
The brazilnut albumin Seed Pearson etc., Plant Mol.Biol.18:235-245,1992.
Legumin Seed Ellis etc., Plant Mol. Biol.10:203-214,1988.
Gluten (rice) Seed Takaiwa etc., Mol.Gen.Genet.208:15-22,1986; Takaiwa etc., FEBS Letts.221:43-47,1987.
Zein Seed Plant Mol Biol such as Matzke, 14 (3): 323-32 1990
napA Seed Stalberg etc., Planta 199:515-519,1996.
Wheat LMW and HMW glutenin-1 Endosperm Mol Gen Genet 216：81-90，1989； NAR 17：461-2，1989
Wheat SPA Seed Albani etc., Plant Cell, 9:171-184,1997
Wheat α, β, γ-wheat gliadin Endosperm EMBO J.3：1409-15，1984
Barley Itr1 promotor Endosperm
Barley B1, C, D hordein Endosperm Theor Appl Gen 98：1253-62，1999；Plant J 4：343-55，1993；Mol Gen Genet 250：750-60，1996
Barley DOF Endosperm Mena etc., The Plant Journal, 116 (1): 53-62,1998
blz2 Endosperm EP99106056.7
Synthetic promoter Endosperm Vicente-Carbajosa etc., Plant J.13:629-640,1998.
Rice prolamine NRP33 Endosperm Wu etc., Plant Cell Physiology 39 (8) 885-889,1998
Rice alpha-globulin Glb-1 Endosperm Wu etc., Plant Cell Physiology 39 (8) 885-889,1998
Rice OSH1 Embryo Sato etc., Proc.Natl.Acad.Sci.USA, 93:8117-8122,1996
Rice alpha-globulin REB/OHP-1 Endosperm Plant Mol. Biol.33:513-522 such as Nakase, 1997
Rice ADP-glucose PP Endosperm Trans Res 6：157-68，1997
Corn ESR gene family Endosperm Plant J 12：235-46，1997
Chinese sorghum γ-kafirin Endosperm PMB 32：1029-35，1996
KNOX Embryo Postma-Haarsma etc., Plant Mol. Biol.39:257-71,1999
The rice oleosin Embryo and aleuron Wu etc., J.Biochem., 123:386,1998
Sunflower Receptacle oil albumen Seed (embryo and Cummins etc., Plant Mol.Biol.19:873-876,1992
Dry seeds)
The rice 40S ribosomal protein of inferring A little less than, endosperm
The rice alpha-globulin By force, endosperm
The rice alanine aminotransferase A little less than, endosperm
Trypsin inhibitor ITR1 (barley) A little less than, endosperm
Rice WSI18 Embryo+coerce
Rice RAB21 Embryo+coerce
Rice oleosin 18kd Aleuron+embryo
In order to identify the promotor of functional equivalent, can analyze promotor length and/or the expression pattern of candidate's promotor by for example promotor effectively being connected in reporter gene and in the various plants tissue, measuring expression level and the pattern of reporter gene.Suitable well-known reporter gene comprises for example GRD beta-glucuronidase or beta-galactosidase enzymes.Measure promoter activity by the enzymatic activity of measuring GRD beta-glucuronidase or beta-galactosidase enzymes.Can compare with promotor length and/or expression pattern and with reference to promotor (for example in the inventive method used promotor) then.Alternatively, can be by quantitative mRNA or by the mRNA level of used nucleic acid in the inventive method is compared to measure promotor length with the mRNA level of housekeeping gene such as 18S rRNA, utilize method well known in the art, photodensitometry, real-time quantitative PCR or RT-PCR (Heid etc., 1996Genome Methods 6:986-994) as Northern trace associating autoradiography image." weak promoter " is often referred to low-level driving encoding sequence expression promoter." low-level " refers in each cell about 1/10,000 the transcript transcript to about 1/100,000, the transcript to about 1/500,0000.On the contrary, " strong promoter " drives encoding sequence with high level and expresses, or the transcript of about 1/10 the transcript transcript to about 1/100 to about 1/1,000 in each cell.
Randomly, can also in the construct of introduced plant, use one or more terminator sequences.Term " terminator " comprises control sequence, and it is the dna sequence dna that is positioned at transcription unit's end, transmits 3 ' processing and polyadenylation signal and the transcription termination signal of primary transcript.Other controlling element can comprise transcribes and translational enhancer.One skilled in the art will know that and be applicable to the sequence of implementing terminator of the present invention and enhanser.This class sequence is conventionally known to one of skill in the art or can easily obtains.
Genetic constructs of the present invention can also be included in keeps and/or copies required replication orgin sequence in the particular cell types.An example is situation about genetic constructs need be kept in bacterial cell as additive type genetic elements (as plasmid or clay molecule).Preferred replication orgin includes but not limited to fl-ori and colE1.
But genetic constructs optionally contains selectable marker gene.As used herein, term " but selectable marker gene " comprises any gene of giving cell phenotype, and this gene is expressed in cell, is conducive to identify and/or select through nucleic acid construct transfection of the present invention or cell transformed.
Be the successful transfer of the used nucleotide sequence of detection the inventive method, and/or select to contain the transgenic plant of these nucleic acid, preferably applying marking gene (or reporter gene).Therefore but genetic constructs optionally contains selectable marker gene.As used herein, term " but selective marker ", " but selectable marker gene " or " reporter gene " comprise any gene of giving cell phenotype, this gene is expressed in cell, is conducive to identify and/or select through nucleic acid construct transfection of the present invention or cell transformed.These marker gene can be identified the successful transfer of nucleic acid molecule by a series of different principles.Suitable mark can be selected from the mark of giving microbiotic or Herbicid resistant, and it is introduced new metabolism proterties or allows visual selection.But the example of selectable marker gene comprises the gene of the giving antibiotics resistance (nptII of phosphorylation Xin Meisu and kantlex for example, or the hpt of phosphorylation Totomycin or give for example gene of the resistance of bleomycin, Streptomycin sulphate, tsiklomitsin, paraxin, penbritin, gentamicin, Geneticin (G418), spectinomycin or blasticidin), the gene of conferring herbicide resistance (for example provides the bar of Basta resistance; AroA or the gox of glyphosate resistance is provided or gives for example gene of the resistance of imidazolone, phosphinothricin or sulfacarbamide), perhaps provide the gene of metabolism proterties (for example to allow plant to use seminose as the manA of sole carbon source, or the xylose isomerase of xylose utilization, or anti-nutrition mark is as the resistance to the 2-deoxyglucose).The visable indicia expression of gene causes forming color (for example GRD beta-glucuronidase GUS or beta-galactosidase enzymes and painted substrate, for example X-Gal), luminous (for example luciferin/luciferase system) or fluorescence (green fluorescent protein GFP and derivative thereof).This inventory only represents the possible mark of sub-fraction.The technician is very familiar to this class mark.Preferably use different marks according to different organisms and system of selection.
Known nucleic acid is stablized or integration,temporal advances vegetable cell, and only a few cell is taken in foreign DNA, and is integrated into its genome, and this depends on used expression vector and used rotaring dyeing technology.For identifying and select these intasomies, but the gene of the selective marker (as indicated above those) of will encoding is usually introduced in the host cell with goal gene.These marks can use in mutant, and original these genes do not have function in the described mutant, for example lack by ordinary method.In addition, but the sequence of the nucleic acid molecule of coding selective marker and code book invention or the used polypeptide of the inventive method can be introduced host cell in same carrier, perhaps in independent carrier.By the nucleic acid stability cells transfected of introducing can be for example by select (for example, but be integrated with the cell survival of selective marker and other cells die) identified.
In case successfully introduce nucleic acid, to no longer need or do not expect to exist in the genetically modified host cell marker gene, particularly microbiotic and herbicide resistance gene advantageously adopt the technology that can remove or excise these marker gene so introduce the method for nucleic acid according to the present invention.A kind of such method is the method that is called cotransformation.The cotransformation method adopts two carriers to transform simultaneously, and a carrier carries according to nucleic acid of the present invention, and second carried one or more marker gene.The transformant of larger proportion receive or for plant, contain (up to 40% or above transformant) two carriers.For Agrobacterium-mediated Transformation, transformant receives only the part of carrier usually, is the sequence of T-DNA institute side joint, and it is often referred to expression cassette.Can from transform plant, remove marker gene by hybridization subsequently.In another approach, utilize the marker gene be integrated into transposon to transform (being called the Ac/Ds technology) with the nucleic acid of expectation.Transformant can be hybridized with the transposase source, perhaps with giving the instantaneous or stable conversion transformant of nucleic acid construct that transposase is expressed.At (about 10%) in some cases, in case successfully transform, transposon is jumped out the host cell gene group and is lost.Under some other situation, transposon skips to different positions.In these cases, must eliminate marker gene by hybridization.In the microbiology field, researched and developed technology that might or be convenient to detect this type of event.Another advantageous method depends on the method that is called recombination system, it is advantageous that to exempt the hybridization removal process.Foremost this type systematic is called the Cre/lox system.Cre1 is recombinase, the sequence of its excision between the loxP sequence.If marker gene is incorporated between the loxP sequence, in case successfully transform, because the expression of this recombinase, it is excised.Other recombination systems have HIN/HIX, FLP/FRT and REP/STB system (Tribble etc., J.Biol.Chem., 275,2000:22255-22267; Velmurugan etc., J.Cell Biol., 149,2000:553-566).Might be integrated into Plant Genome according to nucleotide sequence of the present invention locus specificity.These methods also can be applied to microorganism such as yeast, fungi or bacterium naturally.
In preferred embodiments, the gene construct that provides contains:
(i) I class HDZip hox5 nucleic acid or its variant; Or NRT coding nucleic acid or its variant; Or I type shaggy sample kinases coding nucleic acid or its variant;
(ii) can drive the constitutive promoter that (i) amplifying nucleic acid sequence is expressed; With optional
(iii) transcription termination sequence.
Constitutive promoter is preferably the GOS2 promotor, more preferably constitutive promoter is rice GOS2 promotor, also preferred constitutive promoter is shown in the nucleotide sequence that is substantially similar to SEQ ID NO:33 or SEQ IDNO:178, and most preferably constitutive promoter is shown in SEQ ID NO:33 or SEQID NO:178.The present invention also provides the purposes in the methods of the invention of construct as hereinbefore defined.
In another embodiment preferred, the gene construct that provides contains:
(i) YEP16 coding nucleic acid or its variant;
(ii) can drive the seed specific promoters that (i) amplifying nucleic acid sequence is expressed; With optional
(iii) transcription termination sequence.
The present invention also comprises can be by the plant of the inventive method acquisition.Therefore the present invention provides can be by plant, the plant part (comprising vegetable cell) of the inventive method acquisition, described plant or part (comprising cell) I class HDZip hox5 nucleic acid or its variant transgenosis; Or NRT coding nucleic acid or its variant transgenosis; Or YEP16 coding nucleic acid or its variant transgenosis; Or I type shaggy sample kinases coding nucleic acid or its variant transgenosis.
The present invention also provides with respect to corresponding wild-type or other control plant production has the growth characteristics of improvement, the particularly method of the transgenic plant of the productive rate of increase, is included in and introduces and express any nucleic acid for the inventive method as herein described in the plant.
For purposes of the present invention, " genetically modified ", " transgenosis " or " reorganization " refer to, for example, nucleotide sequence, the expression cassette that contains described nucleotide sequence, gene construct or carrier or the organism that transforms with described nucleotide sequence, according to expression cassette of the present invention or carrier, by all that construct of recombination method generation, wherein:
A) coding is used for the nucleic acid sequences to proteins of the inventive method, or
B) effectively be connected in the Genetic Control sequence of the nucleotide sequence according to the present invention, promotor for example, or
C) a) and b)
Be not present in its natural genotypic environment, perhaps modify by recombination method, might modify the form of taking and be for example replacement, interpolation, disappearance, inversion or the insertion of one or more nucleotide residues.Natural genotypic environment is interpreted as natural gene group or the chromosomal loci in the primordial plant or is present among the genomic library.Under the situation of genomic library, preferably keep the natural genotypic environment of nucleotide sequence, keep at least partially.At least be at least 50bp, preferably 500bp, especially preferably 1000bp, 5000bp at least most preferably at least at least at the environment sequence length of nucleotide sequence one side side joint.When naturally occurring expression cassette---for example promotor of nucleotide sequence and coding natural combination that exists between the corresponding nucleic sequence of the protein that is used for the inventive method of definition as mentioned---when non-natural synthetic (" manually ") method such as mutagenic treatment were modified, this expression cassette became transgene expression cassette.For example, suitable method is described in US 5,565,350 or WO 00/15815 in.
More specifically, the invention provides the method for the transgenic plant that produce the growth characteristics (the particularly productive rate of Zeng Jiaing) with improvement, described method comprises:
(i) in plant, plant part or vegetable cell, introduce and express I class HDZip hox5 nucleic acid or its variant; NRT coding nucleic acid or its variant; YEP16 coding nucleic acid or its variant; I type shaggy sample kinases coding nucleic acid or its variant; With
(ii) culturing plants cell under the condition that promotes plant-growth and growth.
Can be with the direct introduced plant cell of nucleic acid or plant itself (comprising any other parts of introducing tissue, organ or plant).According to a preferred aspect of the present invention, preferably by transforming the nucleic acid introduced plant.
The mentioned term " conversion " of this paper comprises exogenous polynucleotide is shifted into host cell, does not consider to shift used method.Can take place or the embryo take place immediately that the plant tissue of clonal expansion can transform with genetic constructs of the present invention by organ, and from its whole plants of regenerating.Concrete tissue is selected and will be become because of the clonal expansion system of the concrete species that can provide and be suitable for transforming most.Exemplary target tissue comprises leaf dish, pollen, embryo, cotyledon, plumular axis, megagamete, callus, existing meristematic tissue (for example apical meristem, axillalry bud and root meristematic tissue), and the meristematic tissue of inducing (for example cotyledon meristematic tissue and plumular axis meristematic tissue).Polynucleotide can be introduced host cell instantaneously or stably, and can, for example keep nonconformable state as plasmid.Alternatively, it can be integrated into host genome.The transformed plant cells that obtains can be then well known to a person skilled in the art that mode is regenerated as plant transformed.
The conversion of plant species is a kind of quite conventional technology at present.Advantageously, can use the arbitrary of multiple method for transformation to introduce goal gene to suitable ancester cell.Method for transformation comprises with the chemical substance of liposome, electroporation, the picked-up of enhancing dissociative DNA, directly bombards, transforms and microinjection with virus or pollen to plant injection DNA, particle gun.Method can be selected from calcium/polyoxyethylene glycol method (Krens, F.A. etc. (1882) Nature 296, the 72-74 for protoplastis; (1987) Plant Mol.Biol.8:363-373 such as Negrutiu I.); The electroporation of protoplastis ((1985) Bio/Technol 3 such as Shillito R.D., 1099-1102); The microinjection of vegetable material ((1986) Mol.Gen Genet 202:179-185 such as Crossway A.); The particle bombardment (KleinT.M. etc. (1987) Nature 327:70) of DNA or RNA bag quilt; (nonconformable) virus infection, etc.Any rice method for transformation of knowing of preferred use, by agriculture bacillus mediated conversion, produce the transgenosis rice plant of expressing gene, the method of in following arbitrary document, describing for example: disclosed European patent application EP 1198985 A1, Aldemita and Hodges (Planta, 199:612-617,1996); Chan etc. (Plant Mol.Biol.22 (3) 491-506,1993), Hiei etc. (Plant is (2) J.6: 271-282,1994), its disclosed content is incorporated this paper into as a reference as abundant elaboration.Transform as for cereal, (Nat.Biotechnol.14 (6): 745-50 such as preferable methods such as Ishida, 1996) or described in the Frame etc. (Plant Physiol.129 (1): 13-22,2002), its disclosed content is incorporated this paper into as a reference as abundant elaboration.
Usually after conversion, select the vegetable cell or the cell mass that there are one or more marks, described mark becomes complete plant with the material regeneration that transforms thereafter by the expressive gene of plant coding that moves with the goal gene corotation.
After DNA shifts and regenerates, can assess and infer plant transformed, for example construct with existence, copy number and/or the genome of Southern assay goal gene.Optionally or extraly, available Northern and/or Western analyze the expression level of (western blotting) or the new DNA of introducing of quantitative PCR monitoring, and all technology all are well known to those of ordinary skill in the art.
The conversion plant that produces can breed in several ways, as the breeding technique by clonal propagation or classics.For example, the first-generation (or T1) but the s-generation (or T2) transformant of plant transformed selfing to obtain isozygotying, and the T2 plant can be further by classical breeding technique breeding.
The inverting biological body that produces can have various ways.For example, they can be the mosaics of transformant and non-transformed cell; Clone's transformant (for example through transforming all cells that comprises expression cassette); The graft of conversion and non-transforming tissue (for example in plant, transforming the rhizome grafting to the scion of non-conversion).
Any vegetable cell or plant that the present invention obviously prolongs and produced by any method described herein, and all plant parts and its propagulum.The present invention also prolongs and contains the elementary conversion that produced by any aforesaid method or the offspring of cells transfected, tissue, organ or whole plants, unique requirement of described offspring be with the inventive method in the parent present identical genotype and/or phenotypic characteristic.
The present invention also comprises the I class HDZip hox5 nucleic acid that contains separation or the host cell of its variant; Contain the NRT coding nucleic acid of separation or the host cell of its variant; Contain the YEP16 coding nucleic acid of separation or the host cell of its variant; Contain the I type shaggy sample kinases coding nucleic acid of separation or the host cell of its variant.Preferred host cell is vegetable cell.
The part that the present invention also prolongs and plant can be gathered in the crops, such as but not limited to: seed, leaf, fruit, flower, stem, rhizome, stem tuber and bulb.The invention still further relates to the product of directly being derived by the part gathered in the crops of such plant, as dried ball or dry powder, oils, fat and lipid acid, starch or protein.
The present invention also comprises the purposes of I class HDZip hox5 nucleic acid or its variant and the purposes of I class HDZiphox5 polypeptide or its homologue; The purposes of the purposes of NRT coding nucleic acid or its variant and NRT polypeptide or its homologue; The purposes of the purposes of YEP16 coding nucleic acid or its variant and YEP16 polypeptide or its homologue; The purposes of I type shaggy sample kinases coding nucleic acid or its variant and the purposes of I type shaggy sample kinase polypeptide or its homologue.Such purposes relates to improvement any plant growth characteristics as hereinbefore defined.
Can in the procedure of breeding, use I class HDZip hox5 nucleic acid or its variant or I class HDZip hox5 polypeptide or its homologue; NRT coding nucleic acid or its variant or NRT polypeptide or its homologue; YEP16 coding nucleic acid or its variant or YEP16 polypeptide or its homologue; I type shaggy sample kinases coding nucleic acid or its variant or I type shaggy sample kinase polypeptide or its homologue are wherein identified the dna marker that can be connected in one of aforementioned gene or its variant hereditarily.Can use described nucleic acid or variant or polypeptide or its homologue to define molecule marker.Can in the procedure of breeding, use this DNA or protein labeling then, have the plant of the growth characteristics (as the productive rate that increases) of improvement with selection.Described nucleic acid or variant are as hereinbefore defined.
I class HDZip hox5 nucleic acid/gene; NRT coding nucleic acid/gene; YEP16 coding nucleic acid/gene; The allele variant of I type shaggy sample kinases coding nucleic acid/gene also can be used for the auxiliary procedure of breeding of mark.This class procedure of breeding needs to use for example EMS mutagenesis sometimes, introduces allele variant by the plant mutagenic treatment; Alternatively, this program can begin with the allele variant of collecting what is called " natural " origin that is not intended to generation.Identify allele variant by for example PCR then.Be to select step subsequently, in order to select the better allele variant of the sequence of discussing, described allele variant provides the growth characteristics of improvement, as the productive rate that increases.Generally the growth behavior that contains the plant of studying the different allele variants of sequence to some extent by monitoring is selected, for example the different allele variants of any nucleic acid/gene for the inventive method as herein described.Can in greenhouse or field, monitor growth behavior.More optional step comprise and will contain plant and the other plant hybridization of better allele variant through evaluation.For example, can make the combination that produces the purpose phenotypic characteristic in this way.
Aforementioned nucleic acid and variant can also be as probes, are used for carrying out heredity and physical mapping for the part of those genes and as the gene of its chain proterties mark.Such information can be used in plant breeding, to obtain having the strain of expectation phenotype.This class of described nucleic acid or variant is used the nucleotide sequence that only needs to grow to few 15 Nucleotide.Described nucleic acid or variant can be used as restriction fragment length polymorphism (RFLP) mark.Available described nucleic acid or variant are surveyed the Southern trace (Sambrook J, Fritsch EF and Maniatis T (1989) " molecular cloning: laboratory manual ") of the plant genome DNA of restriction digest.Use computer program such as MapMaker (Lander etc. (1987) Genomics 1:174-181) that the banding pattern that produces is carried out genetic analysis subsequently, to make up genetic map.In addition, can use the Southern trace of the genomic dna that restriction enzyme that nuclei acid probe contains one group of individuality handles, described one group individually is the parent of the clear and definite genetic cross (genetic cross) of representative and one group of individuality of filial generation.The separation of record dna polymorphism, and be used for to calculate formerly position (Botstein etc. (1980) Am.J.Hum.Genet.32:314-331) with nucleic acid shown in the genetic map of this colony's acquisition or variant.
Derive generation and the purposes of probe of the plant gene that uses in genetic mapping is described among Bematzky and Tanksley (1986) the Plant Mol.Biol.Reporter 4:37-41.Described in numerous publications with aforesaid method or its flexible form specific cDNA clone was carried out genetic mapping.For example, can use F2 hybridization colony, backcross population, panmictic population, the homogenic system of close relative and the mapping of other group of individuals.These class methods are that those skilled in the art are well-known.
Nucleic acid probe also can be used for physical mapping and (namely settle sequence at physical map; See Hoheisel etc., In Non-mammalian Genomic Analysis:A Practical Guide, Academicpress 1996,319-346 page or leaf, and the reference of wherein quoting).
In another embodiment, nucleic acid probe can be used for direct fluorescence in situ hybridization (FISH) mapping (Trask (1991) Trends Genet.7:149-154).(several kb are to a hundreds of kb although the method inclination of FISH mapping is at present used big clone; See (1995) Genome Res.5:13-20 such as Laan), but the raising of susceptibility allows to use short probe in the FISH mapping.
The multiple method based on nucleic acid amplification that is used for heredity and physical mapping can use described nucleic acid to carry out.Example comprises the polymorphism (CAPS of allele specific amplification (Kazazian (1989) J.Lab.Clin.Med11:95-96), pcr amplified fragment; Sheffield etc. (1993) Genomics16:325-332), allele-specific connects (Landegren etc. (1988) Science 241:1077-1080), Nucleotide extension (Sokolov (1990) Nucleic Acid Res.18:3671), radiation hybridization mapping (Walter etc. (1997) Nat.Genet.7:22-28) and Happy mapping (Dear and Cook (1989) Nucleic Acid Res.17:6795-6807).For implementing these methods, it is right to use the nucleotide sequence design and produce the primer that is used for amplified reaction or primer extension reaction.This class primer design is that those skilled in the art are well-known.Use the method for the genetic mapping of PCR-based, may need to identify that leap is corresponding to the dna sequence dna difference between the parent of nucleotide sequence of the present invention zone mapping.Yet this is dispensable usually to drawing method.
The method according to this invention is had the plant of the growth characteristics of improvement as previously mentioned.The growth characteristics of these improvement can also make up other favourable proterties economically, as other volume increase proterties, the proterties of tolerance, the multiple structural attitude of improvement and/or biological chemistry and/or physiologic character to other biological and abiotic stress.
Description of drawings
Refer now to the following drawings and describe the present invention, wherein:
Fig. 1 has shown that the multiple ratio of I class HDZip homeodomain of different plant origins is right, use be VNTI AlignX multiple ratio to program, based on improved ClustalW algorithm (InforMax, Bethesda, MD, Http:// www.informaxinc.com), adopting default setting, the open point penalty in room is 10, it is 0.05 that point penalty is extended in the room.The constant amino acid L of homeodomain 16, W 48, F 49, N 51And R 53Vertically adding frame table shows.I class HDZip preference amino acid A 46And W 56Equally vertically adding frame table shows.DNA marks with black surround above comparison in conjunction with necessary 3 spirals.6 seven peptides separate with vertical line.7 position called after a, b, c, d, e, f and g positions in each seven peptide.Leu is positioned at the d position of each seven peptide, and vertically adds frame table and show.
Fig. 2 has shown that the multiple ratio of a plurality of plant I class HDZip hox5 polypeptide is right, use be VNTI AlignX multiple ratio to program, based on improved ClustalW algorithm (InforMax, Bethesda, MD, Http:// www.informaxinc.com), adopting default setting, the open point penalty in room is 10, it is 0.05 that point penalty is extended in the room.3 primary structure territory severes that characterize from the N-end to the C-end direction add frame table and show, and are accredited as acid box, I class homeodomain and 6 seven peptide leucine zippers.In addition, Trp tail and RPFF amino acid motif slightly add frame table and show.
Fig. 3 has shown binary vector, is used for expressing the rice I class HDZip hox5 that is under the control of GOS2 promotor rice.
Fig. 4 has described the example of I class homeodomain leucine zipper (HDZip) the hox5 sequence that is used for enforcement the inventive method in detail.A plurality of sequences come from public EST splicing (seeing Table A), and sequencing quality is lower.Therefore may there be the situation that minority nucleic acid replaces in expection.Nucleotide sequence defines with initial (ATG) and terminator codon.
Fig. 5 has shown the typical structure domain structure that is used for the NRT albumen of the inventive method, and this sentences SEQ ID NO:53 as demonstration.The protein of SEQ ID NO:53 comprises and starts from the MFS_1 structural domain that shows with runic that S69 terminates in F432.The terminal membrane spaning domain (T441 to P463 below lines out) of inferring that exists of this MFS_1 domain C.
Fig. 6 has shown (a) phylogenetic tree, and wherein SEQ ID NO:126 represents rice NRT1 protein sequence, contains the PTR2 structural domain; (b) multiple ratio of sequence is right shown in embodiment 14 Table I.The asterisk of multiple ratio centering represents that amino acid is identical in the sequence of comparing, and conservative the replacement represented in branch, and point is represented time conservative the replacement.
Fig. 7 has shown binary vector, is used for expressing the rice NRT encoding histone nucleic acid that is under the control of GOS2 promotor rice.
Fig. 8 has described in detail and has been used for the example that (except SEQ ID NO:125 and SEQ ID NO:126) implements the NRT sequence of the inventive method.SEQ ID NO:125 and SEQ ID NO:126 sequence are not full length sequence probably.
Fig. 9 has shown binary vector, is used for expressing the Arabidopis thaliana YEP16 coding nucleic acid that is under the control of oleosin promotor rice.
Figure 10 has shown the YEP16 sequence that is used for the inventive method.
Figure 11 selects from Planta (2003) 218:1-14 (Wang etc.), has shown plant replying abiotic stress.Elementary coercing as arid, salinity, hot and cold and chemical pollution interknits usually, and causes primary cellular defect and secondary coercing, as infiltration and oxidative stress.The initial signal (changing as infiltration and ionic effect or temperature, membrane fluidity) of coercing triggers the downstream signal conductive process and transcribes control, thereby excites stress response mechanism rebuilding stable state, and protection and repair impaired protein and film.Reply deficiency with a plurality of steps for one in signal conduction and the gene activation process, may finally cause the irreversible variation of Cell Homeostasis, and cause the destruction of function and structural protein and film, cause necrocytosis.
Figure 12 selects from TRENDS in plant Science Vol.7, and No.10, has shown shaggy sample kinases path possible in the plant in October, 2002 (Claudia Jonak and Heribert Hirt).AtGSK1 is the positive regulatory factor that high salt is replied.WIG (wound-induced type GSK) involves the conduction of wound signal.AtSK11 and AtSK12 participate in the correct pattern of blooming.Heredity and biochemical analysis have shown plain sterol (BR) the signal conduction of rape of BIN2 (the plain sterol non-sensitive type-1 of rape) mediation, and this is controlling the nuclear accumulation of BES1 (BR11-EMS-repressor 1) and BRZ1 (brassinazole-resistance factor 1).
Figure 13 selects from TRENDS in plant Science Vol.7, No.10, in October, 2002 (Claudia Jonak and Heribert Hirt), shown that the system of (a) 10 kinds of Arabidopis thaliana Glycogen Synthase kinase 3s/SHAGGY sample protein kinase (GSK) analyzes.Arabidopis thaliana GSK can be classified as 4 hypotypes.(b) phylogenetic tree of the total length GSK cDNA of the different plant species that obtain from blast search repeatedly.Colea BSK θ (Y12674); Alfalfa MSK1 (X68411), MSK2 (X68410), MSK3 (X68409), MSK4 (AF432225), WIG (AJ295939); Tobacco NSK6 (Y08607), NSK59 (AJ002315), NSK91 (AJ224163), NSK111 (AJ002314), NTK-1 (X77763); Rice OSK γ (AB59612), OSK η (Y13437); Petunia PSK4 (X83619), PSK6 (AJ224164), PSK7 (AJ224165), SPK6 (X83620).Comparison utilizes Clustal X to carry out as outgroup (out-group) with Arabidopis thaliana MPK1 (Q39021) by contiguous connection method; Phylogenetic tree is with the TreeView programdesign.
Figure 14 has shown that the kinase whose CLUSTAL multiple ratio of plant I type shaggy sample is right.Motif I and motif II add frame table and show.
Figure 15 has shown binary vector, is used for expressing the rice I type shaggy sample kinases that is under the control of GOS2 promotor rice.
Figure 16 has described the example of the I type shaggy sample kinase sequence that is used for enforcement the inventive method in detail.
Embodiment
Refer now to following examples and describe the present invention, described embodiment only is intended to illustrate, but not is intended to limit up hill and dale or otherwise limit the scope of the invention.
Unless otherwise indicated, recombinant DNA technology is according to being described in (Sambrook (2001) " molecular cloning: laboratory manual ", the third edition, cold spring harbor laboratory publishes, the cold spring port, New York) or Ausubel etc. (1994), Current Protocols in Molecular Biology, the standard method of the Current Protocols first roll and second volume is carried out.The standard material of plant molecular work and method are described in Plant Molecular Biology Labfax (1993) by R.D.D.Croy, and BIOS ScientificPublications Ltd (UK) and Blackwell Scientific Publications (UK) publish.
Example I class HDZip hox5 polypeptide and encoding sequence
The evaluation of embodiment 1:SEQ ID NO:1 and SEQ ID NO:2 correlated series
Utilize database sequence research tool such as basic local comparison instrument (BLAST) (Altschul etc. (1990) J.Mol.Biol.215:403-410; With (1997) Nucleic Acids Res.25:3389-3402 such as Altschul) in the sequence of the Entrez of American National biotechnology information center (NCBI) Nucleotide database maintenance, identify sequence (full-length cDNA, EST or genome).Usually blast program is by comparing nucleic acid or peptide sequence with sequence library, and by calculating the significance,statistical of coupling, is used for having between the searching sequence zone of local similar.For example, nucleic acid SEQ IDNO:1 encoded polypeptide is used the TBLASTN algorithm, use default setting, open strainer, to ignore the low complex degree sequence.The output form of analyzing is for comparing in twos, and sorts according to probability score (E value), and wherein score value reflects the occurrent probability of specific comparison (the E value is more low, and the significance of hit event is more high).Except the E value, also keep the score to relatively carrying out identity per-cent.Identity per-cent refers to that two compare the number of the identical Nucleotide (or amino acid) on length-specific between nucleic acid (or polypeptide) sequence.In some cases, can adjust default parameter with the strict degree of change search.For example, can increase the E value to show the match event of low strict degree.Can identify the short event of almost accurately mating in this way.
Following Table A provides the nucleotide sequence tabulation relevant with the nucleotide sequence of SEQ ID NO:1.
Table A: the example of the sequence relevant with the nucleotide sequence of SEQ ID NO:1
Title NCBI Nucleotide accession number Nucleotide SEQ ID NO The polypeptide SEQ ID NO of translation The source
Orysa_hox5 XM 482406 1 2 Rice
Orysa_hox16 XM 467603 3 4 Rice
Zeama_hox5 ＊ CO458693 DV024016 5 6 Zea mays
Zeama_hox16 AY105265 7 8 Zea mays
Sacof_hox5 ＊ CA088615 CA115362 CA142506 9 10 Sugarcane (Saccharum officinarum)
Sorbi_hox5 ＊ BE363386 CD432381 11 12 Dichromatism chinese sorghum
Triae_hox16 ＊ DR735359 DR741379 13 14 Wheat
CD916488
Arath_ATHB1 X58821 15 16 Arabidopis thaliana
Dauca_CHB3 ＊＊ D26575 17 18 Radix Dauci Sativae
Glyma_HD157 ＊＊ AF184278 19 20 Soybean
Crapl_CPHB-5 AF443621 21 22 Craterostigma plantagineum
Goshi_hox5 ＊ DT465649 CD486134 23 24 Upland cotton
Lyces_hox5 BT014213.1 25 26 Tomato
Lyces_VaHOX1 X94947 27 28 Tomato
Medsa_hox16 ＊ CB892061 CA858059 29 30 Alfalfa
Aqufo_hox5 DT758247
31 32 Aquilegia formosa x Aquilegia pubescens
Poptr_hox16_1 scaff_XV.439 40 41 Trembling poplar
Poptr_hox16_2 scaff_XII.649 42 43 Trembling poplar
Poptr_hox16_3 lcl|scaff_VIII.1839 44 45 Trembling poplar
Medtr_hox161 CR954197.2 46 47 The puncture vine clover
Phavu_hox16 AF402605 48 49 Kidney bean (Phaseolus vulgaris)
Lotco_hox16 AP006364 50 51 Root or stem of Littleleaf Indianmulberry (Lotus corniculatus)
*Contig by a plurality of EST accession number (having shown main EST) splicing; The EST sequencing quality is lower usually, and expection may have minority nucleic acid and replace.
*From the sequence of Radix Dauci Sativae and soybean, compare with its accession number and to correct.
The comparison of embodiment 2:I class HDZip hox5 peptide sequence
(it is based on popular gradual comparison Clustal algorithm (Thompson etc. (1997) Nucleic Acids Res25:4876-4882 for Ying Jun company, AlignX Invitrogen) from Vector NTI in use; Chenna etc. (2003) Nucleic Acids Res 31:3497-3500).Can utilize the contiguous clustering algorithm constructing system that connects to set.Default value is the open point penalty 10 in room, and point penalty 0,1 is extended in the room, and the weight matrix of selecting is Blosum 62 (if comparison polypeptide).
The comparison of described multiple sequence the results are shown in Fig. 2.3 primary structure territory severes that characterize from the N-end to the C-end direction add frame table and show, and are accredited as acid box, I class homeodomain and 6 seven peptide leucine zippers." conserved domain " comprises this 3 structural domains.In addition, Trp tail and RPFF amino acid motif slightly add frame table and show.
The calculating of overall identity per-cent between the embodiment 3:I class HDZip hox5 peptide sequence
Overall similarity between the total length I class HDZip hox5 peptide sequence and identity per-cent utilize matrix overall comparison instrument (MatGAT) software (BMC Bioinformatics.2003 4:29.MatGAT:an application that generates similarity/identity matrices usingprotein or DNA sequences.Campanella JJ, Bitincka L, Smalley J; Software by Ledion Bitincka trustship) determine.MatGAT software need not data are compared in advance, can produce the similarity/identity matrix of DNA or protein sequence.This program is utilized Myers and Miller overall comparison algorithm, and (the open point penalty in room is 12, and to extend point penalty be 2 in the room) carry out a series of comparison in twos, utilize for example Blosum 62 (for polypeptide) calculating similarity and identity, then the result is arranged in distance matrix.Sequence similarity is shown in the diagonal lines Lower Half, and sequence identity is shown in the diagonal lines first half.
More used parameter has:
Matrix: Blosum 62 keeps the score
First room: 12
Extend the room: 2
The overall similarity of peptide sequence length range (the part of polypeptide sequence is foreclosed) and the software analysis of identity the results are shown in table B1.The diagonal lines top provides identity per-cent, and the diagonal lines below provides similarity per-cent.
Compare with SEQ ID NO:2, the identity percentages show between the peptide sequence can be low to moderate 29%.
Table B1: the overall similarity of peptide sequence length range and the MatGAT result of identity
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
1.Aqufo_Hox5 56 54 37 34 64 36 64 35 34 36 47 37 36 38 38 36 42 41 46 49 42
2.Arath_ATHB1 73 52 34 34 59 36 57 33 34 36 44 34 35 40 35 35 39 39 41 43 39
3.Crapl_CPHB-5 69 66 33 35 56 37 59 33 33 33 45 39 34 37 36 36 41 39 41 44 41
4.Dauca_CHB3 52 52 48 44 39 53 35 46 49 46 30 33 47 58 56 43 32 33 31 33 33
5.Glyma_HD157 50 47 48 58 33 44 32 43 43 72 33 31 84 48 48 47 32 31 31 31 32
6.Goshi_Hox5 79 74 71 53 49 38 64 36 36 37 46 38 35 39 36 35 40 39 46 49 40
7.Lotco_Hox16 51 53 51 66 62 53 35 45 66 50 29 31 49 62 59 49 30 31 30 32 31
8.Lyces_Hox5 75 70 72 51 45 75 50 34 34 36 46 38 34 37 36 33 41 41 45 47 41
9.Lyces_VaHOX1 49 48 47 63 58 48 62 47 45 44 31 32 47 53 49 44 33 33 32 33 33
10.Medtr_Hox16 48 48 50 65 64 49 78 48 63 46 30 30 45 59 55 42 31 30 31 31 30
11.Medtr_Hox16_1 52 49 50 61 81 49 67 49 61 64 33 28 77 51 48 50 32 31 29 29 32
12.Orysa_Hox16 62 59 58 50 50 60 47 58 45 50 51 49 34 32 31 30 46 45 73 76 45
13.Orysa_Hox5 53 47 52 48 48 52 48 50 44 45 46 59 32 32 32 30 66 66 49 50 65
14.Phavu_HOX16 51 51 48 64 89 49 65 47 63 65 88 49 48 56 55 51 34 32 31 32 33
15.Poptr_HOX16_1 54 54 52 71 66 52 75 50 66 73 69 48 49 71 92 48 35 35 32 34 34
16.Poptr_HOX16_2 51 49 51 70 66 50 73 49 65 70 66 47 46 71 96 47 34 33 32 32 34
17.Poptr_HOX16_3 52 51 47 59 59 52 63 45 59 59 62 44 44 65 63 63 34 33 30 31 33
18.Sacof_Hox5 62 58 57 47 44 60 48 57 44 45 46 56 69 46 48 47 47 95 46 46 94
19.Sorbi_Hox5 62 57 55 46 45 58 51 58 45 44 46 56 69 47 50 47 46 97 43 46 94
20.Triae_Hox16 62 54 56 48 48 59 47 58 47 49 47 82 61 48 52 51 46 56 55 72 45
21.Zeama_Hox16 63 58 59 51 49 62 51 60 49 50 47 81 62 51 49 48 46 56 57 81 45
22.Zeama_Hox5 62 58 56 46 44 59 49 57 45 45 45 55 68 46 50 46 48 96 96 57 56
As hereinbefore defined, " conserved domain " of I class HDZip hox5 peptide sequence comprises acid box, I class homeodomain and 6 seven peptide leucine zipper (see figure 2)s from the N-end to the C-end.When conserved domain rather than full-length polypeptide sequence are carried out the identity percentage analysis, observe identity per-cent and raise, shown in table B2.Compare with SEQ ID NO:2, Schwellenwert is higher than 50% amino acid identity now.
Table B2: the overall similarity of peptide sequence " conserved domain " and identity MatGAT result
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
1.Aqufo_hox5_CD 81 74 66 82 62 82 62 61 75 66 61 67 63 60 68 67 73 76 67
2.Arath_ATHB1_CD 93 74 59 82 61 85 61 61 71 66 63 65 62 59 67 66 71 71 67
3.Crapl_CPHB-5_CD 85 85 61 78 62 77 57 60 69 63 62 62 58 65 65 64 66 68 65
4.Dauca_CHB3_CD 81 79 75 64 70 62 66 69 57 57 64 80 75 66 58 59 57 57 58
5.Goshi_hox5_CD 94 95 89 81 66 83 63 63 74 66 63 68 63 64 67 66 73 75 67
6.Lotco_hox16_CD 80 78 74 81 80 62 67 85 54 52 62 77 73 64 53 52 54 54 53
7.Lyces_hox5_CD 91 92 88 79 92 77 57 61 75 68 63 66 62 60 71 69 75 76 70
8.Lyces_VaHOX1_CD 77 75 71 79 76 84 74 71 56 57 62 73 68 60 58 58 57 57 58
9.Medtr_hox1_6CD 77 75 74 81 77 93 75 84 59 57 61 75 71 62 57 57 58 59 57
10.Orysa_hox16_CD 93 92 85 77 92 79 92 74 75 84 58 60 58 58 82 82 94 96 82
11.Orysa_hox5_CD 90 87 83 78 88 77 88 71 74 91 59 58 57 57 92 93 81 84 94
12.Phavu_hox16_CD 79 79 75 80 79 81 76 75 80 76 75 71 71 65 60 60 57 58 60
13.Poptr_hox16_1_CD 81 80 75 88 82 90 79 84 87 79 80 86 93 65 59 60 58 61 59
14.Poptr_hox16_2_CD 79 77 74 85 79 87 76 82 84 75 75 85 98 62 57 57 56 58 57
15.Poptr_hox163CD 74 75 73 80 75 76 71 76 77 73 71 78 77 77 57 57 56 58 58
16.Sacof_hox5_CD 89 86 80 77 87 77 86 71 73 88 96 74 79 75 72 98 79 82 98
17.Sorbi_hox5_CD 89 86 81 77 87 77 87 71 73 89 97 74 79 76 72 99 78 82 98
18.Triae_hox16_CD 93 92 85 79 92 80 92 74 75 98 91 77 80 77 73 88 89 95 79
19.Zeama_hox16_CD 93 92 86 78 92 81 92 75 77 98 92 79 80 76 74 90 91 98 82
20.Zeama_hox5_CD 89 86 81 77 87 77 87 71 73 89 97 74 79 75 72 99 100 89 91
The evaluation of the structural domain that embodiment 4:I class HDZip hox5 peptide sequence comprises
Protein families, structural domain and site (Integrated Resource of ProteinFamilies, Domains and Sites (the InterPro)) database of reallocating resources is the integrated interface that carries out based on the search of text and sequence tag database commonly used.The InterPro database gets up these database combination, and they utilize diverse ways to learn and about the diversified angle bioinformation of abundant profiling protein matter, to obtain protein tag.The cooperation database comprises SWISS-PROT, PROSITE, TrEMBL, PRINTS, ProDom and Pfam, Smart and TIGRFAMs.Interpro is by European information biology institute (the European Bioinformatics Institute) trustship that is positioned at Britain.
The InterPro scanning result of peptide sequence shown in the SEQ ID NO:2 is shown in table C.
The InterPro scanning result of peptide sequence shown in the table C:SEQ ID NO:2
InterPro IPR000047 Helix-turn-helix motif, λ sample repressor
PRINTS PR00031 HTHREPRESSR
InterPro IPR001356 Same source capsule (homeobox)
PRODOM PD000010 Homeobox
PRINTS PR00024 HOMEOBOX
PFAM PF00046 Homeobox
SMART SM00389 HOX
PROFILE PS00027 HOMEOBOX_1
PROFILE PS50071 HOMEOBOX_2
InterPro IPR003106 Leucine zipper is correlated with source capsule
PFAM PF02183 HALZ
InterPro IPR009057 The homeodomain sample
SUPERFAMILY SSF46689 The homeodomain sample
InterPro IPR012287 Homeodomain is correlated with
GENE3D G3DSA：1.10.10.60 Homeodomain is correlated with
Whether be rich in the one-level amino acid of specific amino acids (for example acid box) and form the software program, particularly ProtParam instrument (2003) ExPASy:the proteomics server for in-depthprotein knowledge and analysis.Nucleic Acids Res 31:3784-3788 such as () Gasteiger E that (in %) can be used to from the ExPASy server and calculate in order to determine the polypeptide structure territory.The composition of desired polypeptides sequence and the average amino acid composition (in %) in the Swiss-Prot protein sequence database can be compared then.
Following table (table D) compares the mean value in the %Asp (D) in the acid box of SEQ ID NO:2, %Glu (E) and combined content and the Swiss-Prot protein sequence database.
Table D
％Asp(D) ％Glu(E) ％Asp(D)+％Glu(E)
Mean value in the Swiss-Prot protein sequence database 5.3％ 6.6％ 11.9％
The acid box of SEQ ID NO:2 9.1％ 54.5％ 63.6％
Acid box can be the part in the transcriptional activation domain.According to the aminoacids content in eukaryotic transcription activation structure territory it is classified, and main classification comprises that acidity, glutamine are rich in proline(Pro) and is rich in activation structure territory (Rutherford etc. (2005) Plant is (5): 769-88 J.43, and wherein reference).
Gene ontology opinion (GO) federation is the international cooperation between the various biological database scientist, and its editorial office build European information biology institute in.The target of GO is to provide controlled glossary for molecular function, bioprocess and the cellular component of describing gene product.When carrying out InterPro scanning mentioned above, we also search for the GO database.The molecular function that I class HDZip hox5 peptide sequence has be transcription factor activity and sequence specific DNA in conjunction with activity, and be arranged in the nucleus ((table E) sees the following form) of vegetable cell.
Table E
Gene ontology opinion record
Homeodomain Molecular function: transcription factor activity (GO:0003700) cellular component: nucleus (GO:0005634) molecular function: sequence specific DNA is in conjunction with (GO:0043565)
Leucine zipper, Molecular function: DNA is in conjunction with (GO:0003677)
Be correlated with source capsule Cellular component: nucleus (GO:0005634)
The topology prediction of embodiment 5:I class HDZip hox5 peptide sequence
Utilize professional software such as 2ZIP to carry out leucine zipper prediction and the evaluation of seven peptides, this software is with the simulation of standard coiled coil prediction algorithm and characteristic leucine repeating unit search (Bornberg-Bauer etc. (1998) the Computational Approaches to Identify LeucineZippers that combines, Nucleic Acids Res., 26 (11): 2740-2746, by the Marx's Planck institute that is positioned at German Golm (Max Planck Institut) trustship).Utilize this software can identify potential leucine zipper, leucine repeating unit and coiled coil.
The leucine zipper contained prediction of I class HDZip hox5 peptide sequence has at least 5, preferred 6 seven peptides.When the polypeptide to SEQ ID NO:2 moves this algorithm, find that potential leucine joins and be positioned at the 143-178 position, shown in hereinafter output (numeral amino acid position, C are represented the coiled coil zone, and L represents the leucine of seven peptide inside):
1---------11---------21---------31---------41---------51-----
MDPGRVVFDSGVARRACPGGAQMLLFGGGGSANSGGFFRGVPAAVLGMDESRSSSSAAGA
61--------71--------81--------91--------101--------111-------
GAKRPFFTTHEELLEEEYYDEQAPEKKRRLTAEQVQMLERSFEEENKLEPERKTELARRL
121-------131--------141--------151---------161-------171-----
GMAPRQVAVWFQNRRARWKTKQLEHDFDRLKAAYDALAADHHALLSDNDRLRAQVISLTE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
L------L------L------L------L------L
LZLZLZLZLZLZLZLZLZLZLZLZLZLZLZLZLZLZ
181-------191-------201-------211-------221-------231-------
KLQDKETSPSSATITTAAQEVDQPDEHTEAASTTGFATVDGALAAPPPGHQQPPHKDDLV
CCCCCCCC
241-------251-------261-------271-------281-------291-------
SSGGTNDDGDGGAAVVVFDVTEGANDRLSCESAYFADAAEAYERDCAGHYALSSEEEDGG
301-------311-------321-------331-------341-------
AVSDEGCSFDLPDAAAAAAAMFGAAGVVHHDAADDEEAQLGSWTAWFWS
The mensuration of embodiment 6:I class HDZip hox5 peptide sequence
I class HDZip hox5 polypeptide or its homologue have dna binding activity, preferred combination is at the overlapping 5bp half site of center position, be CAA (A/T) ATTG, as detected in the yeast one-hybrid test (Meijer etc. (2000) Mol Gen Genet 263:12-21).In the instantaneous measurement of rice cell suspending liquid, bombard I class HDZip hox5 polypeptide jointly with gus reporter gene and it is reported and cause the dyeing speck number to increase that these spot colors are darker (Meijer etc., the same) also.This mensuration can be used for proving the incitant function of I class HDZip hox5 polypeptide or its homologue.
Embodiment 7: the clone of rice I class HDZip hox5 nucleotide sequence
Use rice seedling cDNA library (Ying Jun company, Paisley, Britain) as template, by pcr amplification rice I class HDZip hox5 nucleotide sequence.The RNA that extracts from seedling clones cDNA among the pCMV Sport 6.0 after reverse transcription.The average inset size in this storehouse is 1.6kb, and the order of magnitude of original clone's number is 1.67 * 10 7Cfu.6 * 10 10After the amplification first time of cfu/ml, determine that original titre is 3.34 * 10 6Cfu/ml.Extract after the plasmid, the 200ng template is used for 50 μ lPCR mixtures.The pcr amplification the primer is prm06000 (SEQ ID NO:34; Justice is arranged, and initiator codon is runic, and the AttB1 site is italic: 5 '- CTTAAACA
Figure S200680049144XD00672
GATCCCGGCCG 3 ') and prm06001 (SEQ ID NO:35; Reverse complemental, AttB2 site are italic: 5 '
Figure S200680049144XD00673
GATCAGCTCCAGAACCAGG 3 '), they comprise the AttB site of carrying out the Gateway reorganization.Under standard conditions, use the HifiTaq archaeal dna polymerase to carry out PCR.PCR fragment with standard method amplification and purifying 1116bp (comprises the attB site equally; From beginning to terminating as 1050bp).Then carry out the first step of Gateway operation, i.e. BP reaction is recombinated to produce (according to the Gateway term) in vivo with PCR fragment and pDONR201 plasmid during this period and " is entered the clone ".As
Figure S200680049144XD00674
The plasmid pDONR201 of a technology part is available from Ying Jun company.
Embodiment 8: vector construction
Use subsequently and contain entering the clone and carrying out the LR reaction together for the appointment carrier that rice transforms of described nucleotide sequence.This carrier comprises such functional element in the T-DNA border: the selectable mark of plant; The marker expression box that can screen; Be intended to and be cloned into the aim sequence that enters among the clone and carry out the Gateway expression cassette of recombinating in the LR body.The rice GOS2 promotor (SEQID NO:33 or SEQ ID NO:178) that is used for constitutive expression is positioned at the upstream of this Gateway box.
After the LR reconstitution steps, according to method well-known in the art expression vector (Fig. 3) conversion that produces is entered agrobacterium strains LBA4044.
Embodiment 9: Plant Transformation
Rice transforms
With the Agrobacterium-mediated Transformation rice plant that contains expression vector.The ripe dry seeds shelling that stalk rice growing kind Japan is fine.By in 70% ethanol, hatching 1 minute, then at 0.2%HgCl 2In 30 minutes, the usefulness that continues distillation washing 6 times carried out disinfection in each 15 minutes.Aseptic seed is sprouted on the substratum that contains 2,4-D (callus inducing medium).After around hatching in the dark, downcut the callus that embryogenetic scultellum derives and in identical substratum, breed.After two weeks, breed callus in other words by the other 2 all propagation of succeeding transfer culture in same medium.Before cultivating altogether 3 days, upload to be commissioned to train at fresh culture and support embryo's generation callus lines (in order to promote the cell fission activity).
The agrobacterium strains LBA4404 that will contain described expression vector is used for cultivating altogether.Agrobacterium is inoculated in to contain in the suitable antibiotic AB substratum and at 28 ℃ and cultivated 3 days.Then collect bacterium and be suspended in liquid altogether in the culture medium to about 1 optical density(OD) (OD600).Then suspension is transferred to culture dish, and callus was dipped in suspension 15 minutes.Subsequently callus is stained with driedly at filter paper, is transferred to altogether substratum of solid, and hatched 3 days in 25 ℃ in the dark.In the presence of selective agent, altogether the callus of cultivating is containing in the substratum of 2,4-D around 28 ℃ of dark cultivations.During this period, grow the resistant calli island (resistant callus islands) of quick growth.This substance transfer is hatched under illumination to regeneration culture medium, demonstrate embryo's generation potentiality and grow in ensuing four to five weeks and sprout.Bud is downcut and hatched during containing the substratum of growth hormone for 2 to 3 weeks from callus, with its from media transfer to soil.The bud of hardening is cultivated in the greenhouse under the condition in high humidity and short daytime.
Produce about 35 T0 rice transformant independently by a construct.Elementary transformant is transferred to the greenhouse from tissue culture room.Behind the copy number of quantitative PCR analysis checking T-DNA inset, only keep that selective agent is shown single copy transgenic plant of tolerance in order to gather in the crops the T1 seed.After transplanting three to five months, gather in the crops seed.This method provides the ratio of single locus transformant to surpass 50% (Aldemita and Hodges 1996; Chan etc., 1993; Hiei etc., 1994).
Embodiment 10: the phenotype appraisal procedure
10.1 assessment arranges
About 35 T0 rice transformant have independently been produced.Elementary transformant is transferred to greenhouse growth and results T1 seed by tissue culture room.7 events are kept, and wherein T1 separated for 3: 1 that transgenosis existence/shortage takes place.By the expression of monitoring visable indicia, in these events, respectively select about 10 T1 seedling that contain transgenosis (heterozygote and homozygote), and about 10 T1 seedling that lack transgenosis (invalid zygote).Transgenic plant and corresponding invalid zygote be growth side by side on random site.Greenhouse experiment is short daytime (illumination in 12 hours), 28 ℃ in the daytime, 22 ℃ of nights, relative humidity 70%.
According to T1 generation identical appraisal procedure in T2 generation of all T1 events, further assessed.From sowing time to the ripening stage, plant is repeatedly by the digital image-forming case.On each time point, every strain plant is obtained digital image (2048 * 1536 pixels, 1,000 6 hundred ten thousand looks) from least 6 different angles.
The salt stress screening
The plant of the matrix cultivation of being made by coconut fiber and argex (3 to 1 ratios) from 4 events (T2 seed).Sprigging first two weeks to the greenhouse is being used normal nutritive medium.After first two weeks, add the salt (NaCl) of 25mM in the nutritive medium, till the results plant.
The arid screening
In flowerpot soil, cultivate the plant from 5 events (T2 seed) under normal operation, up to entering heading stage.Then it is transferred to " drying " zone, stop to irrigate.In the flowerpot of selecting at random, insert the humidity detection instrument, with monitoring soil water content (SWC).When SWC is lower than certain threshold value, continue moisturizing from the trend plant, up to reaching normal level again.Then plant is transferred under the normal condition again again.Remaining cultivation (plant maturation, seed results) is identical with the plant of not cultivating under the abiotic stress condition.Repeat screening with the T2 seed of the plant results of cultivating under normal operation and carry out taking turns checking, and need not be from the T2 seed of the plant results of arid screening first.
Nutrition (nitrogen) the availability screening that reduces
Except nutritive medium, in flowerpot soil, cultivating rice plant under the normal condition.With specific nutritive medium flowerpot is watered from the plant transplanting to the maturation, the nitrogen of described nutritive medium (N) content reduces always, low 7 to 8 times usually.Remaining cultivation (plant maturation, seed results) is identical with the plant of not cultivating under the abiotic stress condition.Measure the seed correlation parameter then.
10.2 statistical study: F check
Utilize dual factors ANOVA (variance analysis) as statistical model, the plant phenotype feature is carried out comprehensive assessment.All measuring parameters with all events of all plant of gene transformation of the present invention are carried out the F check.Carry out F and check to check the effect of all transformation events of gene pairs, and check the population effect of gene, also be called " whole genetic effect ".The significance threshold value of true whole genetic effect is set to 5% probability level of F check.If significance F test value points to certain genetic effect, this means to be not only that the existence of gene or location cause difference on the phenotype.
10.3 the parameter of measuring
10.3.1 the measurement of biomass correlation parameter
From sowing time to the ripening stage, plant is repeatedly by the digital image-forming case.On each time point, every strain plant is obtained digital image (2048 * 1536 pixels, 1,000 6 hundred ten thousand looks) from least 6 different angles.
Plant area (leaf biomass in other words) is on the ground determined by the sum of all pixels that counting is different from the ground plant part digital image of background.This value is got same time point from the mean value of the photo of different angle shots, and is converted to the physical surface value of representing with square millimeter (physicalsurface value) by calibration.Experiment shows that the ground plant area of measuring by this method is relevant with the biomass that plant shoot divides.Area reaches the time point value of its maximum leaf biomass plant on the ground.Early stage vigor shows as plant (seedling) the ground area of sprouting three weeks of back.
Also utilize another parameter measurement abiotic stress tolerance in case of necessity: the green degree index after the arid, it provides the index of plant senescence.Green degree index is the ratio (being expressed as %) of yellow pixel during imaging first after the arid.
Be to measure the root correlation parameter, culturing plants is in order to can observe root in specially designed flowerpot at the bottom of having transparent bowl.Taking pictures through at the bottom of the basin with digital camera during the plant-growth.Utilize appropriate software to infer the feature of root by photo, as total projection area (this is relevant with the root cumulative volume), mean diameter and above the length (length of one or more thick roots) of the root of certain rugosity threshold value.The increase of root biomass is expressed as the increase of root total biomass (be measured as plant life in the cycle observed maximum root biomass); Perhaps be expressed as root/hat than the increase of (root/shoot index) (being measured as the ratio of root and bud active growth root quality and bud quality during the phase).
10.3.2 the measurement of seed correlation parameter
Elementary panicle, the counting that results are ripe, pack, stick bar code label, then in baking box in 37 ℃ of dryings three days.With the panicle threshing, collect and count all seeds subsequently.Use air-blast device that full husk and ghost are separated.Discard ghost, again the remaining part of counting.At the analytical balance full husk of weighing.Determine the number of full seed by counting remaining full husk number after separating step.All full husks of gathering in the crops from plant are measured the seed overall yield by weighing.Measure the seed sum of every strain plant by counting from the number of the husk of plant results.Draw thousand seed weight (TKW) from full seed number and the deduction of their gross weight of counting.Harvest index is defined as seed overall yield and ground area (mm in the present invention 2) between ratio, multiply by the factor 10 again 6Each is paniculiform spends sum to be defined as ratio between seed sum and the ripe elementary panicle number in the present invention.The full rate of seed is defined as the ratio (representing with %) that the full seed number accounts for the seed sum in the present invention.
Embodiment 11: the result who expresses the transgenosis rice plant of I class HDZip hox5 nucleic acid under the normal growth condition
The assessment result of expressing the transgenosis rice plant of I class HDZip hox5 nucleic acid under the normal growth condition is shown in table F.Shown the difference percentage between transgenic plant and the corresponding invalid zygote, wherein the P value of F check is lower than 0.05.
Table F: under the normal growth condition
Express the assessment result of the transgenosis rice plant of I class HDZip hox5 nucleic acid
Proterties The different per-cent of T1 generation-difference The different per-cent of T2 generation-difference
The average root diameter 2 1
Thick root length 9 17
The seed overall yield 17 19
The full seed number 15 17
Full rate 7 9
The seed sum 6 9
Each paniculiform number of spending 8 9
TKW 2 2
Harvest index 16 16
Green degree index before blooming 2 4
EXAMPLE l 2: the result who under the salt stress growth conditions, expresses the transgenosis rice plant of the nucleotide sequence that is used for enforcement the inventive method
The assessment result of expressing the transgenosis rice plant of I class HDZip hox5 peptide sequence coding nucleic acid under the salt stress growth conditions is shown in table G.Shown the difference percentage between transgenic plant and the corresponding invalid zygote, wherein the P value of F check is lower than 0.05.
Table G: under the salt stress growth conditions
Express the assessment result of the transgenosis rice plant of I class HDZip hox5 nucleic acid
Proterties The different per-cent of T1 generation-difference
The seed overall yield 41
The full seed number 40
Full rate 30
TKW 1
Harvest index 36
Green degree index before blooming 7
Embodiment 13: the result who expresses the transgenosis rice plant of I class HDZip hox5 nucleic acid under the drought stress growth conditions
The assessment result of expressing the transgenosis rice plant of the nucleotide sequence that is used for enforcement the inventive method under the drought stress growth conditions is shown in table H.Shown the difference percentage between transgenic plant and the corresponding invalid zygote, wherein the P value of F check is lower than 0.05.
Table H: under the drought stress growth conditions
Express the assessment result of the transgenosis rice plant of the nucleotide sequence that is used for enforcement the inventive method
Proterties The different per-cent of T1 generation-difference The different per-cent of T2 generation-difference
The average root diameter 2 1
Thick root length 9 14
The seed overall yield 18 31
The full seed number 21 33
Full rate 15 33
Harvest index 18 34
Green degree index after the arid 2 3
Embodiment NRT polypeptide and coding nucleic acid
The evaluation of embodiment 14:SEQ ID NO:52 and SEQ ID NO:53 correlated series
Utilize database sequence research tool such as basic local comparison instrument (BLAST) (Altschul etc. (1990) J.Mol.Biol.215:403-410; With (1997) Nucleic Acids Res.25:3389-3402 such as Altschul), in the sequence of the Entrez of American National biotechnology information center (NCBI) Nucleotide database maintenance, identify the sequence (full-length cDNA, EST or genome) relevant with SEQ ID NO:52 and/or the protein sequence of being correlated with SEQ ID NO:53.Usually blast program is by comparing nucleic acid or peptide sequence with sequence library, and by calculating the significance,statistical of coupling, is used for having between the searching sequence zone of local similar.For example, nucleic acid SEQ ID NO:52 encoded polypeptide is used the TBLASTN algorithm, use default setting, open strainer, to ignore the low complex degree sequence.The output form of analyzing is for comparing in twos, and sorts according to probability score (E value), and wherein score value reflects the occurrent probability of specific comparison (the E value is more low, and the significance of hit event is more high).Except the E value, also keep the score to relatively carrying out identity per-cent.Identity per-cent refers to that two compare the number of the identical Nucleotide (or amino acid) on length-specific between nucleic acid (or polypeptide) sequence.In some cases, can adjust default parameter with the strict degree of change search.
Except the common core acid sequence that can be obtained by NCBI, we also search for privately owned sequence library according to same procedure mentioned above.
Following Table I provides nucleic acid and the protein sequence tabulation relevant with the protein sequence shown in the nucleotide sequence shown in the SEQ ID NO:52 and the SEQ ID NO:53.
Table I: be used for to implement the inventive method
The nucleotide sequence that nucleotide sequence (SEQ ID NO:52) is relevant and infer polypeptide accordingly
Species The GenBank accession number Protein s EQ ID NO: Nucleic acid SEQ ID NO:
Rice BAA33382 53 52
Reed BAC76606 66 65
Zea mays AAT66252 68 67
Zea mays AAN05088 70 69
Barley AAC49531 72 71
Wheat AAK19519 74 73
Barley AAC49532 76 75
Wheat AAG01172 78 77
Wheat AAL11016 80 79
Barley AAD28364 82 81
Barley AAD28363 84 83
Peach BAD02939 86 85
Peach BAD04063 88 87
Soybean AAC09320 90 89
Lotus japonicus Q9ARC5 92 91
Tobacco CAD89799 94 93
Tomato AAK72402 96 95
The Populus species CAG26716 98 97
Tobacco CAD89798 100 99
Nicotiana gossei (Nicotiana plumbaginifolia) CAA69387 102 101
Tomato AAF00053 104 103
Arabidopis thaliana AAC64170 106 105
Tomato AAF00054 108 107
Colea CAC05338 110 109
Arabidopis thaliana AAY78876 112 111
Arabidopis thaliana NP_172289 114 113
Arabidopis thaliana AAC35884 116 115
Arabidopis thaliana NP_200886 118 117
Arabidopis thaliana AAU05505 120 119
Radix Dauci Sativae AAL99362 122 121
Zea mays CAC87729 124 123
Embodiment 15: the comparison of related polypeptide sequence
(it is based on popular gradual comparison Clustal algorithm (Thompson etc. (1997) Nucleic Acids Res25:4876-4882 for Ying Jun company, AlignX Invitrogen) from Vector NTI in use; Chenna etc. (2003) Nucleic Acids Res 31:3497-3500).Can utilize the contiguous clustering algorithm constructing system that connects to set.Default value is the open point penalty 10 in room, and point penalty 0,1 is extended in the room, and the weight matrix of selecting is Blosum 62 (if comparison polypeptide).
Identify to be used for implement the inventive method polypeptide related polypeptide the multiple sequence comparison the results are shown in Fig. 6.Multiple ratio is to having shown the NRT protein sequence high conservative between the multiple species.The protein (be example with SEQ ID NO:126) that contains the PTR2 structural domain does not obviously belong to NRT protein type defined herein.
The calculating of overall identity per-cent between the embodiment 16:NRT peptide sequence
Utilize MatGAT (matrix overall comparison instrument) software (BMC Bioinformatics.20034:29.MatGAT:an application that generates similarity/identity matrices usingprotein or DNA sequences.Campanella JJ for overall similarity and identity per-cent between the full-length polypeptide sequence of implementing the inventive method, Bitincka L, Smalley J; Software by Ledion Bitincka trustship) determine.MatGAT software need not data are compared in advance, can produce the similarity/identity matrix of DNA or protein sequence.This program is utilized Myers and Miller overall comparison algorithm, and (the open point penalty in room is 12, and to extend point penalty be 2 in the room) carry out a series of comparison in twos, utilize for example Blosum 62 (for polypeptide) calculating similarity and identity, then the result is arranged in distance matrix.Sequence similarity is shown in the diagonal lines Lower Half, and sequence identity is shown in the diagonal lines first half.
More used parameter has:
Matrix: Blosum 62 keeps the score
First room: 12
Extend the room: 2
The overall similarity of peptide sequence length range (the part of polypeptide sequence is foreclosed) and the software analysis of identity the results are shown in Table I.The diagonal lines top provides identity per-cent, and the diagonal lines below provides similarity per-cent.
Compare with SEQ ID NO:53, surpass 60% amino acid identity (but may have exception) usually for the identity percentages show between the peptide sequence of implementing the inventive method; And the protein (the rice nitrate transport protein shown in SEQ ID NO:126, following table the 31st row) that contains the PTR2 structural domain only shows extremely limited sequence identity (17% or lower) with NRT albumen.
Figure S200680049144XD00781
Embodiment 17: be used for to implement the evaluation of the structural domain that the peptide sequence of the inventive method comprises
Protein families, structural domain and site (InterPro) database of reallocating resources is the integrated interface that carries out based on the search of text and sequence tag database commonly used.The InterPro database gets up these database combination, and they utilize diverse ways to learn and about the diversified angle bioinformation of abundant profiling protein matter, to obtain protein tag.The cooperation database comprises SWISS-PROT, PROSITE, TrEMBL, PRINTS, ProDom and Pfam, Smart and TIGRFAMs.Interpro is by the European information biology institute trustship that is positioned at Britain.
The InterPro scanning result of peptide sequence shown in the SEQ ID NO:53 is shown in table K.
The InterPro scanning result of peptide sequence shown in the table K:SEQ ID NO:53
Database Accession number Login name
Pfam PF07690 MFS_1
The topology of embodiment 18:NRT peptide sequence prediction (Subcellular Localization, striding film ...)
Utilize the Subcellular Localization of TargetP 1.1 prediction eukaryotic proteins.The location comparison is carried out based on the existence of the terminal presequence prediction of arbitrary N-: chloroplast transit peptides (cTP), Mitochondrially targeted peptide (mTP) or secretion path signal peptide (SP).With serve as that the score value predicted of basis is not real probability, and add up and must not be 1.But, the location most probable that score is the highest meets TargetP, and how reliably the relation between the score value (reliability class) can be used as described prediction index.Reliability class (RC) scope from 1 to 5, the wherein the strongest prediction of 1 expression.TargetP is by the server maintenance of Technical University Of Denmark biological sequence analysis center (Center for Biological Sequence Analysis, Technical University ofDenmar).
For the sequence that contains the terminal presequence of N-through prediction, can also predict potential cleavage site.
Selected multiple parameter, the prediction and calculation of biological example body type (non-plant or plant), cutoff value setting (the cutoff value setting of not having, predesignating or the cutoff value setting of user's appointment) and cleavage site (be or not).
TargetP 1.1 analytical resultss of peptide sequence shown in the SEQ ID NO:53 are shown in table L.What select is " plant " organism type, does not stipulate cutoff value, and requires the prediction length of transit peptides.Fail to predict Subcellular Localization.
The TargetP 1.1 of peptide sequence analyzes shown in the table L:SEQ ID NO:53
Length (AA) 533
Chloroplast transit peptides 0.116
The mitochondrial transport peptide 0.060
Secretion path signal peptide 0.008
Other ubcellular targets 0.874
The location of prediction -
Reliability class 2
The transit peptides length of prediction -
Can utilize many other algorithms to carry out such analysis, comprise:
● ChloroP 1.1, by Technical University Of Denmark's trust server;
● protein is sought Subcellular Localization forecasting software (Protein Prowler SubcellularLocalisation Predictor), 1.2 version, by (the Institute for Molecular Bioscience of molecular biosciences institute of Brisbane ,Australia University of Queensland, University of Queensland, Brisbane, trust server Australia);
● PENCE Proteome Analyst PA-GOSUB 2.5, by Canada big Alberta Edmonton Alberta university (University of Alberta, Edmonton, Alberta, trust server Canada);
Utilizing the transbilayer helix number of TMHMM program (Technical University Of Denmark biological sequence analysis center) prediction is 11, and table M sees below.
Table M:
The location The amino acid region that participates in
The outside 1 67
Transbilayer helix 68 90
Inboard 91 129
Transbilayer helix 130 152
The outside 153 155
Transbilayer helix 56 178
Inboard 179 184
Transbilayer helix 185 207
The outside 208 221
Transbilayer helix 222 244
Inboard 245 272
Transbilayer helix 273 295
The outside 296 314
Transbilayer helix 315 332
Inboard 333 352
Transbilayer helix 353 375
The outside 376 379
Transbilayer helix 380 402
Inboard 403 408
Transbilayer helix 409 431
The outside 432 440
Transbilayer helix 441 463
Inboard 464 533
The amino acid no prediction of expecting in the transbilayer helix is at least 242 (if greater than 18, then protein very likely is transmembrane protein).In addition, unlikely there is secretion signal, because in 60 amino acid, there is not the transbilayer helix of prediction, also do not have N-terminus signal sequence (expected numbers, 60 amino acid: 0.00085).
The mensuration of embodiment 19:NRT
Be to determine the translocator activity of NRT, adopt the described nitrate absorption measurement of Tong etc. (Plant J.41,442-450,2005).In brief, preparing mRNA by construct, in described construct, is 5 '-UTR of Xenopus laevis betaglobulin gene before the purpose NRT encoding histone ORF, is 3 '-UTR of described Xenopus laevis betaglobulin gene afterwards.This mRNA is expelled to the ovocyte V phase and VI interim, express purpose NRT albumen afterwards. 15NO 3 -Hatched ovocyte 3 to 12 hours in 18 ℃ in the solution.Wash ovocyte then and in 60 ℃ of dryings.Pass through to measure with mass spectrograph 15N/ 14N recently measures 15The absorption of N enrichment nitrate.Measuring N O 3The suitable assay method of other that absorb is well known to those skilled in the art, for example referring to Filleur etc. ( 15NO 3 -Uptake in roots, FEBSLetters 489,220-224,2001) or (measurement of anion-elicitedcurrents with the two-electrode voltage-clamp method such as Zhou, J.Biol.Chem.275,39894-39899,2000).If desired, can coexpression nar2 gene, to increase nitrate transport.
Alternatively, the activity of NRT albumen or its homologue can be in NRT albumen or its homologue of GOS2 promotor under controlling and measure by expressing in fine in rice growing kind Japan, and this will produce the plant of the seed productive rate of comparing ground biomass with increase and/or increase with corresponding wild-type plant.The increase of this seed productive rate can be weighed in several ways, for example increase of seed gross weight, full seed quantity or seed sum, the increase of the increase of harvest index or each panicle flower.
The clone of nucleotide sequence shown in the embodiment 20:SEQ ID NO:52
Use rice seedling cDNA library (Ying Jun company, Paisley, Britain) as template, by pcr amplification rice NRT gene.The RNA that extracts from seedling clones cDNA among the pCMVSport 6.0 after reverse transcription.The average inset size in this storehouse is 1.5kb, and the order of magnitude of original clone's number is 1.59 * 10 7Cfu.6 * 10 11After the amplification first time of cfu/ml, determine that original titre is 9.6 * 10 5Cfu/ml.Extract after the plasmid, the 200ng template is used for 50 μ l PCR mixtures.The pcr amplification the primer is prm07061 (SEQ ID NO:54; Justice is arranged, and initiator codon is runic, and the AttB1 site is italic: 5 '
Figure S200680049144XD00821
Figure S200680049144XD00831
Gactcgtcgacggtg-3 ') and prm07062 (SEQ ID NO:55; Reverse complemental, terminator codon are runic, and the AttB2 site is italic: AttB2 site in italic:5 '
Figure S200680049144XD00832
Ctcggtcgcagaattgt C-3 '), they comprise the AttB site of carrying out the Gateway reorganization.Under standard conditions, use Hifi TaqDNA polysaccharase to carry out PCR.Same PCR fragment (comprising the attB site) with standard method amplification and purifying 1683bp.Then carry out the first step of Gateway operation, i.e. BP reaction is recombinated to produce (according to the Gateway term) in vivo with PCR fragment and pDONR201 plasmid during this period and " is entered the clone ".As The plasmid pDONR201 of a technology part is available from Ying Jun company.
Embodiment 21: utilize the nucleotide sequence construction of expression vector shown in the SEQ ID NO:52
Use subsequently and enter the clone and carry out the LR reaction together for the appointment carrier that rice transforms.This carrier comprises such functional element in the T-DNA border: the selectable mark of plant; The marker expression box that can screen; Be intended to and be cloned into the aim sequence that enters among the clone and carry out the Gateway expression cassette of recombinating in the LR body.The rice GOS2 promotor (SEQ ID NO:56 1-2188 position Nucleotide, promotor-assortment of genes) that is used for constitutive expression is positioned at the upstream of this Gateway box.
After the LR reconstitution steps, NRT expression vector (Fig. 7) conversion that produces is entered agrobacterium strains LBA4044, transform rice plant subsequently.
Embodiment 22: Plant Transformation
Rice transforms
With the Agrobacterium-mediated Transformation rice plant that contains expression vector.The ripe dry seeds shelling that stalk rice growing kind Japan is fine.By in 70% ethanol, hatching 1 minute, then at 0.2%HgCl 2In 30 minutes, the usefulness that continues distillation washing 6 times carried out disinfection in each 15 minutes.Aseptic seed is sprouted on the substratum that contains 2,4-D (callus inducing medium).After around hatching in the dark, downcut the callus that embryogenetic scultellum derives and in identical substratum, breed.After two weeks, breed callus in other words by the other 2 all propagation of succeeding transfer culture in same medium.Before cultivating altogether 3 days, upload to be commissioned to train at fresh culture and support embryo's generation callus lines (in order to promote the cell fission activity).
The agrobacterium strains LBA4404 that will contain described expression vector is used for cultivating altogether.Agrobacterium is inoculated in to contain in the suitable antibiotic AB substratum and at 28 ℃ and cultivated 3 days.Then collect bacterium and be suspended in liquid altogether in the culture medium to about 1 optical density(OD) (OD600).Then suspension is transferred to culture dish, and callus was dipped in suspension 15 minutes.Subsequently callus is stained with driedly at filter paper, is transferred to altogether substratum of solid, and hatched 3 days in 25 ℃ in the dark.In the presence of selective agent, altogether the callus of cultivating is containing in the substratum of 2,4-D around 28 ℃ of dark cultivations.During this period, grow the resistant calli island of quick growth.This substance transfer is hatched under illumination to regeneration culture medium, demonstrate embryo's generation potentiality and grow in ensuing four to five weeks and sprout.Bud is downcut and hatched during containing the substratum of growth hormone for 2 to 3 weeks from callus, with its from media transfer to soil.The bud of hardening is cultivated in the greenhouse under the condition in high humidity and short daytime.
Produce about 35 T0 rice transformant independently by a construct.Elementary transformant is transferred to the greenhouse from tissue culture room.Behind the copy number of quantitative PCR analysis checking T-DNA inset, only keep that selective agent is shown single copy transgenic plant of tolerance in order to gather in the crops the T1 seed.After transplanting three to five months, gather in the crops seed.This method provides the ratio of single locus transformant to surpass 50% (Aldemita and Hodges 1996; Chan etc., 1993; Hiei etc., 1994).
Embodiment 23: the phenotype appraisal procedure
23.1 assessment arranges
About 35 T0 rice transformant have independently been produced.Elementary transformant is transferred to greenhouse growth and results T1 seed by tissue culture room.5 events are kept, and wherein T1 separated for 3: 1 that transgenosis existence/shortage takes place.By the expression of monitoring visable indicia, in these events, respectively select about 10 T1 seedling that contain transgenosis (heterozygote and homozygote), and about 10 T1 seedling that lack transgenosis (invalid zygote).Transgenic plant and corresponding invalid zygote be growth side by side on random site, environment arranges as follows: photoperiod=11.5 hour, day light intensity=30,000 luxs or more, daytime temperature=28 ℃ or higher, nocturnal temperature=22 ℃, relative humidity=60-70%.
According to T1 generation identical appraisal procedure in T2 generation of 4 T1 events, further assessed, more individual but each event is used.From sowing time to the ripening stage, plant is repeatedly by the digital image-forming case.On each time point, every strain plant is obtained digital image (2048 * 1536 pixels, 1,000 6 hundred ten thousand looks) from least 6 different angles.
23.2 statistical study:
Utilize dual factors ANOVA (variance analysis) as statistical model, the plant phenotype feature is carried out comprehensive assessment.All measuring parameters with all events of all plant of gene transformation of the present invention are carried out the F check.Carry out F and check to check the effect of all transformation events of gene pairs, and check the population effect of gene, also be called " whole genetic effect ".The significance threshold value of true whole genetic effect is set to 5% probability level of F check.If significance F test value points to certain genetic effect, this means to be not only that the existence of gene or location cause difference on the phenotype.
In order to check the effect of gene in event, namely the strain specificity effect uses the data set from transgenic plant and corresponding invalid plant to carry out the t check in each event." invalid plant " or " invalid segregant " or " invalid zygote " but be to handle the plant that transgenosis has therefrom been separated in an identical manner with transgenic plant.The feminine gender that also invalid plant can be described as isozygotying transforms plant.The significance threshold setting that t is checked is 10% probability level.The result of some events can be higher than this threshold value or be lower than this threshold value.This is based on such hypothesis, and namely gene only has effect in the position of some in genome, and the generation of this position dependence effect is not rare.This type of genetic effect of this paper is also referred to as " the strain effect of gene (line effect of the gene) ".By t value and t-distribution are relatively obtained the p value, perhaps alternatively by F value and F-distribution are relatively obtained the p value.The p value has then provided the correct probability of null hypothesis (namely not having the transgenosis effect).
Because two kinds of experiments carrying out have overlapping events, therefore carry out combinatory analysis.This can be used for checking the consistence of effect in two kinds of experiments, and if situation if this is really true, thereby it can be used for collecting evidence from two kinds of experiments and increases the credibility of conclusion.The method of using is to consider the method with mixed model of the multilevel hierarchy of data (i.e. experiment-event-segregant).Likelihood ratio by relatively card side's distribution tests to obtain the p value.
23.3 the parameter of measuring
The measurement of biomass correlation parameter
From sowing time to the ripening stage, plant is repeatedly by the digital image-forming case.On each time point, every strain plant is obtained digital image (2048 * 1536 pixels, 1,000 6 hundred ten thousand looks) from least 6 different angles.
Plant area (leaf biomass in other words) is on the ground determined by the sum of all pixels that counting is different from the ground plant part digital image of background.This value is got same time point from the mean value of the photo of different angle shots, and is converted to the physical surface value of representing with square millimeter by calibration.Experiment shows that the ground plant area of measuring by this method is relevant with the biomass that plant shoot divides.Area reaches the time point value of its maximum leaf biomass plant on the ground.Early stage vigor shows as plant (seedling) the ground area of sprouting three weeks of back.
The measurement of seed correlation parameter
Elementary panicle, the counting that results are ripe, pack, stick bar code label, then in baking box in 37 ℃ of dryings three days.With the panicle threshing, collect and count all seeds subsequently.Use air-blast device that full husk and ghost are separated.Discard ghost, again the remaining part of counting.At the analytical balance full husk of weighing.Determine the number of full seed by counting remaining full husk number after separating step.All full husks of gathering in the crops from plant are measured the seed overall yield by weighing.Measure the seed sum of every strain plant by counting from the number of the husk of plant results.Draw thousand seed weight (TKW) from full seed number and the deduction of their gross weight of counting.Harvest index is defined as seed overall yield and ground area (mm in the present invention 2) between ratio, multiply by the factor 10 again 6Each is paniculiform spends sum to be defined as ratio between seed sum and the ripe elementary panicle number in the present invention.The full rate of seed is defined as the ratio (representing with %) that the full seed number accounts for seed sum (or Xiao Hua) in the present invention.Each paniculiform number of spending is the parameter of each paniculiform average Xiao Hua number on the estimation plant, is obtained divided by a panicle (first panicles) number by the seed sum.When vertical one-tenth row are observed, the highest panicle and be regarded as panicle one time, the pedestrian worker that goes forward side by side counting with the overlapping panicle of the highest panicle.
Embodiment 24: the phenotype assessment result of transgenic plant
When as mentioned above seed being analyzed, the contriver finds to have higher seed productive rate with NRT gene construct plant transformed than lacking the genetically modified plant of NRT: be expressed as full seed number (this may be the result that full rate increases at least in part), seed gross weight (increasing by 25%) and harvest index.
T1 is summarised among the table N for the result who obtains in the plant.
Table N:
Difference percentage The p value
The full seed number +48 0.0000
The seed gross weight +54 0.0000
Harvest index +38 0.0000
Flower/panicle +14 0.0003
The seed sum +13 0.0287
These positive findingses obtain in generation again at T2.The data presentation of table O by overall growth per-cent and the corresponding p value of full seed number, seed gross weight and harvest index of data computation of independent strain of T2 generation.To these T2 data with the combinatory analysis of T1 for the result in assess again, and the p value that obtains shows that observed effect is very significant.
Table O:
In addition, transgenic plant also show the increase (area maximum (areamax): T1 is on behalf of+7%, and T2 is on behalf of+4%) of biomass, and this increase is significant (the p value of combinatory analysis: 0.0001).
Embodiment YEP16 polypeptide and coding nucleic acid
Embodiment 25: the gene clone of Arabidopis thaliana YEP16 coding nucleic acid
Use rice seedling cDNA library (Ying Jun company, Paisley, Britain) as template, by pcr amplification Arabidopis thaliana YEP16 encoding gene.The RNA that extracts from seedling clones cDNA among the pCMV Sport 6.0 after reverse transcription.The average inset size in this storehouse is 1.6kb, and the order of magnitude of original clone's number is 1.67 * 10 7Cfu.6 * 10 10After the amplification first time of cfu/ml, determine that original titre is 3.34 * 10 6Cfu/ml.Extract after the plasmid, the 200ng template is used for 50 μ l PCR mixtures.The pcr amplification the primer is prm00735 (SEQ ID NO:144; Justice is arranged, and initiator codon is runic, and the AttB1 site is italic: 5 '-
Figure S200680049144XD00881
Figure S200680049144XD00882
a
Figure S200680049144XD00883
Gatactctctcagcatcc-3 ') and prm00736 (SEQ ID NO:145; Reverse complemental, AttB2 site are italic: 5 '-
Figure S200680049144XD00884
Tgtatcatcaagaaacccaga-3 '), they comprise the AttB site of carrying out the Gateway reorganization.Under standard conditions, use Hifi Taq archaeal dna polymerase to carry out PCR.PCR fragment with standard method amplification and purifying 12173bp (comprises the attB site equally; From beginning to terminating as 1050bp).Then carry out the first step of Gateway operation, i.e. BP reaction is recombinated to produce (according to the Gateway term) in vivo with PCR fragment and pDONR201 plasmid during this period and " is entered the clone ".As
Figure S200680049144XD00885
The plasmid pDONR201 of a technology part is available from Ying Jun company.
Embodiment 26: vector construction
Use subsequently and enter the clone and carry out the LR reaction together for the appointment carrier p00640 that rice transforms.This carrier comprises such functional element in the T-DNA border: the selectable mark of plant; The marker expression box that can screen; Be intended to and be cloned into the aim sequence that enters among the clone and carry out the Gateway expression cassette of recombinating in the LR body.The rice oleosin promotor (SEQID NO:143) that is used for seed-specific expression is positioned at the upstream of this Gateway box.
After the LR reconstitution steps, expression vector (Fig. 9) conversion that produces is entered agrobacterium strains LBA4044, transform rice plant subsequently.Make rice plant's growth of conversion, then the parameter described in the embodiment 27 is studied.
Embodiment 27: in the assessment and the result that are in the rice YEP16 coding nucleic acid under the control of rice oleosin promotor under the normal growth condition
About 15 to 20 T0 rice transformant have independently been produced.Elementary transformant is transferred to greenhouse growth and results T1 seed by tissue culture room.7 events are kept, and wherein T1 separates for the 3:1 that transgenosis existence/shortage takes place.By the expression of monitoring visable indicia, in these events, respectively select about 10 T1 seedling that contain transgenosis (heterozygote and homozygote), and about 10 T1 seedling that lack transgenosis (invalid zygote).According to T1 generation identical appraisal procedure in T2 generation of 4 T1 events, further assessed, more individual but each event is used.
27.1 statistical study: F check
Utilize dual factors ANOVA (variance analysis) as statistical model, the plant phenotype feature is carried out comprehensive assessment.All measuring parameters with all events of all plant of gene transformation of the present invention are carried out the F test.Carry out F and check to check the effect of all transformation events of gene pairs, and check the population effect of gene, also be called " whole genetic effect ".The significance threshold value of true whole genetic effect is set to 5% probability level of F check.If significance F test value points to certain genetic effect, this means to be not only that the existence of gene or location cause difference on the phenotype.
The measurement of seed correlation parameter
Elementary panicle, the counting that results are ripe, pack, stick bar code label, then in baking box in 37 ℃ of dryings three days.With the panicle threshing, collect and count all seeds subsequently.Use air-blast device that full husk and ghost are separated.Discard ghost, again the remaining part of counting.At the analytical balance full husk of weighing.Determine the number of full seed by counting remaining full husk number after separating step.All full husks of gathering in the crops from plant are measured the seed overall yield by weighing.Measure the seed sum of every strain plant by counting from the number of the husk of plant results.Draw thousand seed weight (TKW) from full seed number and the deduction of their gross weight of counting.Harvest index is defined as seed overall yield and ground area (mm in the present invention 2) between ratio, multiply by the factor 10 again 6Each is paniculiform spends sum to be defined as ratio between seed sum and the ripe elementary panicle number in the present invention.
Full seed number, seed overall yield (seed gross weight), the full rate of seed (it multiply by 100 for the full seed number again divided by the seed sum) and harvest index with YEP16 coding nucleic acid transgenic plant transformed are shown in table P.Compare with corresponding control plant, these parameters significantly increase in generation at T1.In T2 generation, also observe the average increase of identical parameters.
Table P: usefulness YEP16 coding nucleic acid transgenic plant transformed
T1 is for full seed number, seed gross weight, full rate and harvest index result
Phenotype T1 assessment: show the event number that increases The P value of F check
The seed gross weight All 3 strains demonstrate 22% average increase Significantly
The full seed number All 3 strains demonstrate 22% average increase Significantly
The full rate of seed All 3 strains demonstrate 15% average increase Significantly
Harvest index All 3 strains demonstrate 18% average increase Significantly
Example I type shaggy sample kinases and coding nucleic acid
Embodiment 28: gene clone
Use Arabidopis thaliana seedling cDNA library (Ying Jun company, Paisley, Britain) as template, by pcr amplification rice I type shaggy sample kinases encoding gene.The RNA that extracts from seedling clones cDNA among the pCMV Sport 6.0 after reverse transcription.The average inset size in this storehouse is 1.5kb, and the order of magnitude of original clone's number is 1.59 * 10 7Cfu.6 * 10 11After the amplification first time of cfu/ml, determine that original titre is 9.6 * 10 5Cfu/ml.Extract after the plasmid, the 200ng template is used for 50 μ lPCR mixtures.The pcr amplification the primer is prm5797 (SEQ ID NO:179; Justice is arranged, and initiator codon is runic, and the AttB1 site is italic: 5 '-
Figure S200680049144XD00901
Figure S200680049144XD00902
Ggttcagtaggggttg-3 ') and prm5798 (SEQ ID NO:180; Reverse complemental, terminator codon are runic, and the AttB2 site is italic: 5 '-
Figure S200680049144XD00903
Figure S200680049144XD00904
Agctgtctcatactcctgc-3 '), they comprise the AttB site of carrying out the Gateway reorganization.Under standard conditions, use Hifi Taq archaeal dna polymerase to carry out PCR.Same PCR fragment with standard method amplification and purifying 1328bp.Then carry out the first step of Gateway operation, i.e. BP reaction is recombinated to produce (according to the Gateway term) in vivo with PCR fragment and pDONR201 plasmid during this period and " is entered the clone ".As
Figure S200680049144XD00911
The plasmid pDONR201 of a technology part is available from Ying Jun company.
Embodiment 29: vector construction
Use subsequently and enter the clone and carry out the LR reaction together for the appointment carrier that rice transforms.This carrier comprises such functional element in the T-DNA border: the selectable mark of plant; The marker expression box that can screen; Be intended to and be cloned into the aim sequence that enters among the clone and carry out the Gateway expression cassette of recombinating in the LR body.The rice GOS2 promotor that is used for constitutive expression be positioned at the upstream of this Gateway box (De Pater etc., Plant J.1992Nov; 2 (6): 837-44).
After the LR reconstitution steps, the expression vector (Figure 15) that produces transformed enter agrobacterium strains LBA4044, enter rice plant as conversion as described in the embodiment 30 subsequently.
Embodiment 30: rice transforms
Ripe dry seeds shelling with stalk rice growing kind.By in 70% ethanol, hatching 1 minute, then at 0.2%HgCl 2In 30 minutes, the usefulness that continues distillation washing carried out disinfection in 6 * 15 minutes.Aseptic seed is sprouted on the substratum that contains 2,4-D (callus inducing medium).After around hatching in the dark, downcut the callus that embryogenetic scultellum derives and in identical substratum, breed.After two weeks, breed callus in other words by the other 2 all propagation of succeeding transfer culture in same medium.Before cultivating altogether 3 days, upload to be commissioned to train at fresh culture and support embryo's generation callus lines (in order to promote the cell fission activity).The agrobacterium strains LBA4404 that utilization contains double base T-DNA carrier carries out common cultivation.Agrobacterium is inoculated in to contain in the suitable antibiotic AB substratum and at 28 ℃ and cultivated 3 days.Then collect bacterium and be suspended in liquid altogether in the culture medium to about 1 optical density(OD) (OD600).Then suspension is transferred to culture dish, and callus was dipped in suspension 15 minutes.Subsequently callus is stained with driedly at filter paper, is transferred to altogether substratum of solid, and hatched 3 days in 25 ℃ in the dark.In the presence of selective agent, altogether the callus of cultivating is containing in the substratum of 2,4-D around 28 ℃ of dark cultivations.During this period, grow the resistant calli island of quick growth.This substance transfer is hatched under illumination to regeneration culture medium, demonstrate embryo's generation potentiality and grow in ensuing four to five weeks and sprout.Bud is downcut and hatched during containing the substratum of growth hormone for 2 to 3 weeks from callus, with its from media transfer to soil.The bud of hardening is cultivated in the greenhouse under the condition in high humidity and short daytime.After transplanting three to five months, gather in the crops seed.This method provides the ratio of single locus transformant to surpass 50% (Aldemita and Hodges, Planta, 199612-617,1996; Chan etc., Plant Mol.Biol.22 (3) 491-506,1993, Hiei etc.; Plant J., 6 (2) 271-282,1994).
Embodiment 31: the salt stress screening
Sow seed, and select seedling by the expression of monitoring visable indicia.After planting 10 days, sprigging in the plastic tub of diameter 12cm, is filled 1: 1 wet sand and vermiculite mixture in the basin.Soak into flowerpot with clear water before transplanting.Then seedling is transplanted to greenhouse growth and results T1 seed by tissue culture room.Transplant and flowerpot placed under the salt condition in back 1 day.Every day four inferior to 8am, 12pm, 4pm and 9pm with contain 25mM NaCl and hereinafter the salt stress nutritive medium of listed component flowerpot is watered:
● NPK nutritional blend 20-20-20Peters professional (Ohio, USA Mary Si Weier city's Scott (Scotts) company), concentration 1kg/m 3
● chelated magnesium Chelal Mg (the BMS company of Belgian Bai Nan), 333.33ml/m 3
● chelating ion Libfer (Britain Bradford CIBA company), 21.67g/m 3
●NaCl 1.425kg/m 3
Be monitoring salt concn in basis with the week, and carry out salt when being necessary and add.Culturing plants begins to enrich up to grain under these conditions.Then it is transferred to compartments different in the greenhouse, water with clear water every day, up to the results seed.As describing in detail, records embodiment 32 growth and productivity parameters.
Embodiment 32: assessment and result
About 15 to 20 T0 rice transformant have independently been produced.Elementary transformant is transferred to greenhouse growth and results T1 seed by tissue culture room.At least 5 events are kept, and wherein T1 separated for 3: 1 that transgenosis existence/shortage takes place.By the expression of monitoring visable indicia, in these events, respectively select about 10 T1 seedling that contain transgenosis (heterozygote and homozygote), and about 10 T1 seedling that lack transgenosis (invalid zygote).According to T1 generation identical appraisal procedure in T2 generation of 4 T1 events of putting up the best performance, further assessed, more individual but each event is used.
Statistical study: F check
Utilize dual factors ANOVA (variance analysis) as statistical model, the plant phenotype feature is carried out comprehensive assessment.All measuring parameters with all events of all plant of gene transformation of the present invention are carried out the F test.Carry out F and check to check the effect of all transformation events of gene pairs, and check the population effect of gene, also be called " whole genetic effect ".The significance threshold value of true whole genetic effect is set to 5% probability level of F check.If significance F test value points to certain genetic effect, this means to be not only that the existence of gene or location cause difference on the phenotype.
32.1 the measurement of seed correlation parameter
The elementary panicle that results are ripe, pack, stick bar code label, then in baking box in 37 ℃ of dryings three days.With the panicle threshing, collect and count all seeds subsequently.Use air-blast device that full husk and ghost are separated.Discard ghost, again the remaining part of counting.At the analytical balance full husk of weighing.All full husks of gathering in the crops from plant are measured the seed overall yield by weighing.Harvest index is defined as seed overall yield and ground area (mm in the present invention 2) between ratio, multiply by the factor 10 again 6
Table has as a result hereinafter shown the p value of the F check of T1 and T2 assessment.Also shown difference percentage between transgenosis and the corresponding invalid zygote (or not containing genetically modified plant).For example, for the seed gross weight in T1 generation, 2 events are the seed gross weight positive (namely under condition of salt stress, compare with the seed weight of corresponding invalid zygophyte, being shown as the seed gross weight increases (＞54%, wherein p value＜0.1938 of F check)).In generation, 1 event is the seed gross weight positive (namely under condition of salt stress, compare with the seed weight of corresponding invalid zygophyte, being shown as the seed gross weight increases (65%, wherein the p value of F check is 0.0252)) at T2.
Table Q: the T1 under the condition of salt stress and T2 generation
Shaggy sample kinases transgenic plant and corresponding non-transgenic plant
Phenotype T1 Difference The p value T2 Difference The p value
The area maximum 1 event 47％ 0.0254 1 event 19％ 0.0951
The full seed number 1 event 58％ 0.0376 1 event 61％ 0.0313
The seed sum 2 events 41％ ＜0.191 1 event 28％ 0.0999
Each paniculiform number of spending 1 event 55％ 0.0177 1 event 26％ 0.0072
The seed gross weight 2 events ＞54％ ＜0.1938 1 event 65％ 0.0252
TKW 1 event 6％ 0.1008
Harvest index 2 events ＞57％ ＜0.0627 1 event 54％ 0.0086
Be used for the sequence of the inventive method to the conversion of corn, wheat, soybean, rape, clover
Corn transforms
With (1996) Nature Biotech 14 (6) such as Ishida: the evolutionary approach of the described method of 745-50 is carried out the conversion of Semen Maydis.Transforming in corn is that genotype is dependent, and has only the special genes type to transform and to regenerate.Inbred lines A188 (University of Minnesota (University ofMinnesota)) or be that parent's hybrid is the outstanding source that transforms donor material with A188, but also can successfully use other genotype.About 11 days of pollination back (DAP) when the length of immature embryo is about 1 to 1.2mm the time, gathers in the crops fringe from maize plant.Immature embryo and the agrobacterium tumefaciens (Agrobacterium tumefaciens) that contains expression vector are carried out common cultivation, and by organ transgenic plant take place to reclaim.The embryo that downcuts is successively at callus inducing medium with contain on the corn regeneration culture medium of selective agent (as imidazolone, but can use the multiple choices mark) and cultivate.Culture plate is hatched 2-3 week under illumination in 25 ℃, or sprouts up to growth.From each embryo, green bud transferred on the maize rooting substratum and at 25 ℃ and hatch 2-3 week, up to growing root.The bud that to take root is transplanted in the soil in greenhouse.Produce the T1 seed by selective agent being shown plant tolerance and that contain single copy T-DNA inset.
Wheat transforms
By (1996) Nature Biotech 14 (6) such as Ishida: the described method of 745-50 is carried out the conversion of wheat.Transform cultivar Bobwhite commonly used (can obtain from Mexico CIMMYT company).Immature embryo and the agrobacterium tumefaciens that contains expression vector are carried out common cultivation, and by organ transfer-gen plant takes place to reclaim.After embryo and Agrobacterium hatch, successively external at callus inducing medium with contain on the regeneration culture medium of selective reagents (for example imidazolone, but can use the multiple choices mark) and cultivate.Culture plate is hatched 2-3 week under illumination in 25 ℃, or sprouts up to growth.From each embryo, green bud transferred on the root media and at 25 ℃ and hatch 2-3 week, up to growing root.The bud that to take root is transplanted in the soil in greenhouse.Produce the T1 seed by selective agent being shown plant tolerance and that contain single copy T-DNA inset.
Soybean transforms
According to agro-industrial (the Texas A﹠amp of university in the Texas; M) patent US 5,164, the evolutionary approach soybean transformation of method described in 310.Some commercial soybean varieties can transform by this method.Cultivar Jack (deriving from Illinois seeds company (the Illinois Seed foundation)) is generally used for transforming.Soybean seeds is carried out disinfection to be used for external sowing.From seven day age seedling cut off hypocotyl, radicle and a cotyledon.The cotyledon of further cultivating epicotyl and being left is to grow the armpit knot.Downcutting these armpits ties and hatches with the agrobacterium tumefaciens that contains expression vector.After cultivating processing altogether, the washing explant is also transferred to and is selected in the substratum.Downcut the bud of regeneration and place the bud elongation medium.The bud that length is no more than 1cm places root media up to growing root.The bud that to take root is transplanted in the soil in greenhouse.Produce the T1 seed by selective agent being shown plant tolerance and that contain single copy T-DNA inset.
Semen Brassicae campestris/rape transforms
Utilize cotyledon petiole and the hypocotyl of seedling in 5-6 days ages to carry out tissue culture as explant, and transform according to (1998, Plant Cell Rep 17:183-188) such as Babic.Commercial cultivar (Canada's agricultural (Agriculture Canada)) is as the standard variety that transforms, but also can use other kind.Brassica seed is carried out surface sterilization be used for external sowing.From external seedling, cut off the cotyledon petiole explant that has adhered to cotyledon, and by inoculating Agrobacterium (containing expression vector) in the cut end immersion bacterial suspension with petiole explant.Then with explant in the MSBAP-3 substratum that contains 3mg/l BAP, 3% sucrose, 0.7% plant gel (Phytagar) in 23 ℃, 16 hours illumination cultivation 2 days.After cultivating 2 days altogether with Agrobacterium, petiole explant transferred in the MSBAP-3 substratum that contains 3mg/l BAP, cefotaxime, Pyocianil or Ticarcillin/Clavulanate Acid (300mg/l) and cultivated 7 days, cultivate up to shoot regeneration at the MSBAP-3 substratum with cefotaxime, Pyocianil or Ticarcillin/Clavulanate Acid and selective agent then.When the long 5-10mm of bud, with its cutting-out and transfer in the bud elongation medium (MSBAP-0.5 contains 0.5mg/l BAP).The bud that about 2cm is long is transferred to root media (MS0) and is carried out root induction.The bud that to take root is transplanted in the soil in greenhouse.Produce the T1 seed by selective agent being shown plant tolerance and that contain single copy T-DNA inset.
Clover transforms
Utilize the method for 1999Plant Physiol 119:839-847 such as () McKersie to transform the regeneration clone of clover (alfalfa).The regeneration of clover and conversion are that genotype is dependent, therefore need regeneration plant.Obtain existing description of method of regeneration plant.For example, these can be selected from cultivar Rangelander (Canada agricultural) or any other as Brown DCW and the described commercial alfalfa variety of A Atanassov (1985.Plant Cell Tissue Organ Culture 4:111-112).Alternatively, select RA3 kind (winconsin university (University of Wisconsin)) to carry out tissue culture (Walker etc., 1978Am J Bot 65:654-659).Petiole explant is carried out common cultivation with the overnight culture (McKersie etc., 1999PlantPhysiol 119:839-847) or the LBA4404 that contain the agrobacterium tumefaciens C58C1pMP90 of expression vector.Explant is being contained 288mg/LPro, 53mg/L Thioproline, 4.35g/L K 2Cultivated altogether in the dark 3 days on the SH inducing culture of SO4 and 100 μ m Syringylethanones.With explant at half intensity Murashige-Skoog substratum (Murashige and Skoog, 1962) washing in, and place identical SH inducing culture, this substratum not to contain Syringylethanone but contain suitable selective agent and suitable microbiotic to suppress the Agrobacterium growth.After several weeks, somatic embryo transferred to the BOi2Y that does not contain growth regulator, does not contain microbiotic, contains 50g/L sucrose and grow in the substratum.Somatic embryo is sprouted at half intensity Murashige-Skoog substratum.The sprigging that to take root is grown in basin and in the greenhouse.Produce the T1 seed by selective agent being shown plant tolerance and that contain single copy T-DNA inset.
Sequence table
Sequence table
＜110〉Cropdesign NV
＜120〉has the plant and preparation method thereof of improvement growth characteristics
<130>PF57967
<150>EP 05110413.1
<151>2005-11-07
<150>EP 05110429.7
<151>2005-11-07
<150>US 60/736,194
<151>2005-11-14
<150>EP 05110900.7
<151>2005-11-17
<150>US 60/739,686
<151>2005-11-23
<150>EP 05111260.5
<151>2005-11-24
<150>US 60/742,287
<151>2005-12-05
<160>181
<170>PatentIn version 3.3
<210>1
<211>1050
<212>DNA
＜213〉rice (Oryza sativa)
<400>1
atggatcccg gccgcgtcgt gttcgactcc ggcgtggcgc ggcgggcgtg ccccggcggc 60
gcgcagatgc ttctcttcgg cggcggcggc agcgccaaca gcggcggctt cttccgaggc 120
gtgccggcgg cggtgctggg gatggatgaa tcgcggtcgt cgtcgtcggc ggcgggggcg 180
ggggcgaagc ggccgttctt cacgacgcac gaggagctcc tggaggagga gtactacgac 240
gagcaggcgc cggagaagaa gcggcggctg acggcggagc aggtgcagat gctggagcgg 300
agcttcgagg aggagaacaa gctggagccg gagcggaaga cggagctcgc ccgccgcctc 360
ggcatggccc cccggcaggt cgccgtctgg ttccagaacc gccgcgcccg ctggaagacc 420
aagcagctcg agcacgactt cgaccgcctc aaggccgcct acgacgccct cgccgccgac 480
caccatgccc tcctctccga caacgaccgc ctccgcgcgc aggtaatctc attaaccgag 540
aagctgcaag acaaggagac gtcgccgtcg tcggcgacca tcaccaccgc ggcgcaggag 600
gtcgaccagc cggacgaaca cacggaggcc gcgtcaacca ccggcttcgc caccgtcgac 660
ggcgcattgg cggcgccacc gcccggccac cagcagccgc cgcataaaga tgatcttgtg 720
agcagcggcg gcaccaacga cgacggcgat ggcggcgcgg ccgtggtggt cttcgacgtc 780
accgagggcg ccaacgaccg cctcagctgc gagtcggcgt acttcgccga cgccgcggag 840
gcgtacgagc gcgactgcgc cgggcactac gccctctcgt cggaggagga ggacggcggc 900
gcggtcagcg acgagggctg cagcttcgac ctccccgacg ccgccgccgc cgccgccgcc 960
atgttcggcg ccgccggagt tgtgcaccac gacgccgcgg acgacgagga ggcgcagctc 1020
ggcagctgga ccgcctggtt ctggagctga 1050
<210>2
<211>349
<212>PRT
＜213〉rice
<400>2
Met Asp Pro Gly Arg Val Val Phe Asp Ser Gly Val Ala Arg Arg Ala
1 5 10 15
Cys Pro Gly Gly Ala Gln Met Leu Leu Phe Gly Gly Gly Gly Ser Ala
20 25 30
Asn Ser Gly Gly Phe Phe Arg Gly Val Pro Ala Ala Val Leu Gly Met
35 40 45
Asp Glu Ser Arg Ser Ser Ser Ser Ala Ala Gly Ala Gly Ala Lys Arg
50 55 60
Pro Phe Phe Thr Thr His Glu Glu Leu Leu Glu Glu Glu Tyr Tyr Asp
65 70 75 80
Glu Gln Ala Pro Glu Lys Lys Arg Arg Leu Thr Ala Glu Gln Val Gln
85 90 95
Met Leu Glu Arg Ser Phe Glu Glu Glu Asn Lys Leu Glu Pro Glu Arg
100 105 110
Lys Thr Glu Leu Ala Arg Arg Leu Gly Met Ala Pro Arg Gln Val Ala
115 120 125
Val Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu
130 135 140
His Asp Phe Asp Arg Leu Lys Ala Ala Tyr Asp Ala Leu Ala Ala Asp
145 150 155 160
His His Ala Leu Leu Ser Asp Asn Asp Arg Leu Arg Ala Gln Val Ile
165 170 175
Ser Leu Thr Glu Lys Leu Gln Asp Lys Glu Thr Ser Pro Ser Ser Ala
180 185 190
Thr Ile Thr Thr Ala Ala Gln Glu Val Asp Gln Pro Asp Glu His Thr
195 200 205
Glu Ala Ala Ser Thr Thr Gly Phe Ala Thr Val Asp Gly Ala Leu Ala
210 215 220
Ala Pro Pro Pro Gly His Gln Gln Pro Pro His Lys Asp Asp Leu Val
225 230 235 240
Ser Ser Gly Gly Thr Asn Asp Asp Gly Asp Gly Gly Ala Ala Val Val
245 250 255
Val Phe Asp Val Thr Glu Gly Ala Asn Asp Arg Leu Ser Cys Glu Ser
260 265 270
Ala Tyr Phe Ala Asp Ala Ala Glu Ala Tyr Glu Arg Asp Cys Ala Gly
275 280 285
His Tyr Ala Leu Ser Ser Glu Glu Glu Asp Gly Gly Ala Val Ser Asp
290 295 300
Glu Gly Cys Ser Phe Asp Leu Pro Asp Ala Ala Ala Ala Ala Ala Ala
305 310 315 320
Met Phe Gly Ala Ala Gly Val Val His His Asp Ala Ala Asp Asp Glu
325 330 335
Glu Ala Gln Leu Gly Ser Trp Thr Ala Trp Phe Trp Ser
340 345
<210>3
<211>1197
<212>DNA
＜213〉rice
<400>3
atggagtccg gccggctcat cttcagcacg gcgggctccg gcgccgggca gatgctcttc 60
ttggactgcg gcgctggcgg cggcggcgtc ggcggcgggg ccatgttcca tcgaggggcg 120
agaccggtgc tcggcatgga ggaaggaggg cgcggcgtca agcggccctt cttcaccacc 180
cccgacgagc tcctcgaaga ggagtactac gacgagcagc tcccggagaa gaagcggcgc 240
ctcacgccgg agcaggtgca tctgctggag aggagcttcg aggaggagaa caagctggag 300
ccggagcgga agacggagct ggcgcggaag ctagggctgc agccgcggca ggtcgccgtg 360
tggttccaga accgccgcgc gcgctggaag accaagcagc tcgagcgcga cttcgaccgc 420
ctcaaggcgt cgttcgacgc cctccgcgcc gaccacgacg ccctcctcca ggacaaccac 480
cgcctccact ctcaggtcat gtcgttgacc gagaagctgc aagagaagga gacgacgacc 540
gagggcagcg ccggcgcggc cgttgacgtc ccgggcttgc ctgcggcggc cgacgtgaag 600
gtcgccgtcc cggacgccga ggaaccggcg ctggaggagg cggcggcggc gttcgaggag 660
cagcaggagc agcaggtgaa ggccgaggac aggctgagca cgggcagcgg cgggagcgcg 720
gtggtggaca cggacgcgca actggtggtc gggtgcggcc ggcaagcatc tcgccgccgt 780
ggacagcagc gtggagtcgt acttcccggg cggcgacgag taccacgact gcgtgatggg 840
ccccatggac cacgccgcgg ggggcatcca gtcggaggag gacgacggcg ccggcagcga 900
cgagggctgc agctactacg ccgacgacgc cggcgtcctc ttcgccgacc acggccacca 960
ccaccaccac caacacgcgg acgacgacga ggaggacggc cagcagatca gctgctggtg 1020
gatgtggaac tagatttctc gcgcgcgcgc gtcgtcgtgc attcaattct cgtgttaaaa 1080
aaatcgttct ctttttcatt tttccgcttc tttgtctgta atgttgagtt tcgatcggct 1140
atgagaagga aggaggtgta tgcatgtgca tggtatggta gggtaacaca tcggtga 1197
<210>4
<211>343
<212>PRT
＜213〉rice
<400>4
Met Glu Ser Gly Arg Leu Ile Phe Ser Thr Ala Gly Ser Gly Ala Gly
1 5 10 15
Gln Met Leu Phe Leu Asp Cys Gly Ala Gly Gly Gly Gly Val Gly Gly
20 25 30
Gly Ala Met Phe His Arg Gly Ala Arg Pro Val Leu Gly Met Glu Glu
35 40 45
Gly Gly Arg Gly Val Lys Arg Pro Phe Phe Thr Thr Pro Asp Glu Leu
50 55 60
Leu Glu Glu Glu Tyr Tyr Asp Glu Gln Leu Pro Glu Lys Lys Arg Arg
65 70 75 80
Leu Thr Pro Glu Gln Val His Leu Leu Glu Arg Ser Phe Glu Glu Glu
85 90 95
Ash Lys Leu Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg Lys Leu Gly
100 105 110
Leu Gln Pro Arg Gln Val Ala Val Trp Phe Gln Asn Arg Arg Ala Arg
115 120 125
Trp Lys Thr Lys Gln Leu Glu Arg Asp Phe Asp Arg Leu Lys Ala Ser
130 135 140
Phe Asp Ala Leu Arg Ala Asp His Asp Ala Leu Leu Gln Asp Asn His
145 150 155 160
Arg Leu His Ser Gln Val Met Ser Leu Thr Glu Lys Leu Gln Glu Lys
165 170 175
Glu Thr Thr Thr Glu Gly Ser Ala Gly Ala Ala Val Asp Val Pro Gly
180 185 190
Leu Pro Ala Ala Ala Asp Val Lys Val Ala Val Pro Asp Ala Glu Glu
195 200 205
Pro Ala Leu Glu Glu Ala Ala Ala Ala Phe Glu Glu Gln Gln Glu Gln
210 215 220
Gln Val Lys Ala Glu Asp Arg Leu Ser Thr Gly Ser Gly Gly Ser Ala
225 230 235 240
Val Val Asp Thr Asp Ala Gln Leu Val Val Gly Cys Gly Arg Gln His
245 250 255
Leu Ala Ala Val Asp Ser Ser Val Glu Ser Tyr Phe Pro Gly Gly Asp
260 265 270
Glu Tyr His Asp Cys Val Met Gly Pro Met Asp His Ala Ala Gly Gly
275 280 285
Ile Gln Ser Glu Glu Asp Asp Gly Ala Gly Ser Asp Glu Gly Cys Ser
290 295 300
Tyr Tyr Ala Asp Asp Ala Gly Val Leu Phe Ala Asp His Gly His His
305 310 315 320
His His His Gln His Ala Asp Asp Asp Glu Glu Asp Gly Gln Gln Ile
325 330 335
Ser Cys Trp Trp Met Trp Asn
340
<210>5
<211>819
<212>DNA
＜213〉Zea mays (Zea mays)
<400>5
atggatccga gcgcggtcag tttcgactct ggcggcgcgc ggcggggcgg cggcgcgcag 60
atgctgctct tcggcggcgg aggcagcgcc aacagcaacg gcttcttccg aggtgttccg 120
atggcggtcc tgggcatgga cgacgcgacg cgcgtgggca agcggccctt cttcacgaca 180
cacgaggagc tcctagagga ggagtactac gacgagcagg cgccggagaa gaagcgccga 240
ctgacggcgg agcaggtgca gctgctggag cggagcttcg aagaagagaa caagctggag 300
ccggagcgca agaccgagct ggctcgccgc ctggggatgg cgccccgcca ggtagctgtt 360
tggttccaga accgccgcgc gcgctggaag accaagcaac tcgagaccga ctatgaccgc 420
ctcaaggctg cttacgacgc actcgccgcc gaccaccagg gcctcctggc cgacaacgat 480
aacctccggg cacaggtgat ctccctgacg gagaagctgc aaggcaagga gacatccccg 540
tcggcaacca ctgctgccca agaggtcgac cagccagacg aacacaccgc tgtgtcaggc 600
acggaagaac tgctggcgca gcagctcaag gacaacctcc acagcagcgg cgactgcact 660
ggccatggca ccctctcttc ggaagaagac gacggtggcg tggtcagtga cgagggctgc 720
agcttcgctc tcccggatgc catgttcgct gccgggttca cccaccatgg cgccgaggag 780
gtgcagctgg ccaactggac atccatgttc tggaactga 819
<210>6
<211>272
<212>PRT
＜213〉Zea mays
<400>6
Met Asp Pro Ser Ala Val Ser Phe Asp Ser Gly Gly Ala Arg Arg Gly
1 5 10 15
Gly Gly Ala Gln Met Leu Leu Phe Gly Gly Gly Gly Ser Ala Asn Ser
20 25 30
Asn Gly Phe Phe Arg Gly Val Pro Met Ala Val Leu Gly Met Asp Asp
35 40 45
Ala Thr Arg Val Gly Lys Arg Pro Phe Phe Thr Thr His Glu Glu Leu
50 55 60
Leu Glu Glu Glu Tyr Tyr Asp Glu Gln Ala Pro Glu Lys Lys Arg Arg
65 70 75 80
Leu Thr Ala Glu Gln Val Gln Leu Leu Glu Arg Ser Phe Glu Glu Glu
85 90 95
Asn Lys Leu Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg Arg Leu Gly
100 105 110
Met Ala Pro Arg Gln Val Ala Val Trp Phe Gln Asn Arg Arg Ala Arg
115 120 125
Trp Lys Thr Lys Gln Leu Glu Thr Asp Tyr Asp Arg Leu Lys Ala Ala
130 135 140
Tyr Asp Ala Leu Ala Ala Asp His Gln Gly Leu Leu Ala Asp Asn Asp
145 150 155 160
Asn Leu Arg Ala Gln Val Ile Ser Leu Thr Glu Lys Leu Gln Gly Lys
165 170 175
Glu Thr Ser Pro Ser Ala Thr Thr Ala Ala Gln Glu Val Asp Gln Pro
180 185 190
Asp Glu His Thr Ala Val Ser Gly Thr Glu Glu Leu Leu Ala Gln Gln
195 200 205
Leu Lys Asp Asn Leu His Ser Ser Gly Asp Cys Thr Gly His Gly Thr
210 215 220
Leu Ser Ser Glu Glu Asp Asp Gly Gly Val Val Ser Asp Glu Gly Cys
225 230 235 240
Ser Phe Ala Leu Pro Asp Ala Met Phe Ala Ala Gly Phe Thr His His
245 250 255
Gly Ala Glu Glu Val Gln Leu Ala Asn Trp Thr Ser Met Phe Trp Asn
260 265 270
<210>7
<211>993
<212>DNA
＜213〉Zea mays
<400>7
atggagtctg gacggctcat cttcaacgcg ccgggctctg gcgccgggca gatgctcttc 60
ctcgactgcg gcgcaggcgg cggtcccggc ggcggcttgt tccatcgagg cgggagaccg 120
atgcttggcc ttgaagaagg gcgcggcgta aaacggccct tcttcacctc gcccgacgag 180
ctcctcgagg aagagtacta cgacgagcag ctgccggaga agaagcgccg cctcacccca 240
gagcaggtgc ttctgctgga gaggagcttc gaggaggaga acaagctgga gccggagcgc 300
aagacggagc tggcgcgcaa gctgggcctg cagcctcgcc aggtggccgt ctggttccag 360
aaccgccgcg cccggtggaa gaccaagcag ctcgagcgcg acttcgaccg cctcaaggcc 420
tccttcgacg ctctccgagc ggaccacgac gccctcctcc aggacaacaa ccgcctccgc 480
tcacaggttg tgtcgttgac cgagaagctg caagagaagg aggatgcgac ggagggcggc 540
gccaccgctg acaccgccgc gccggcggtg gacgtcgagg cttccctggc cgacgacgtc 600
gaggagccag cagagcctgc ggcgacgttc gaggtgctgc aggaggtgaa gtccgaggac 660
aggctgagca ccggcagcgg cgggagcgcg gtggtggacg cggacgcgct gctgtacggc 720
aggttcgccg cggcagttga tagcagcgtg gagtcgtact tccccggcgg cgaggaccac 780
taccacgact gcgggacgat gggccccgtg aatcatggcg ccggaggagg catccagtcg 840
gacgacgacg gcgccggcag cgacgagggg tgcagctact acgccgacga agccgccgcc 900
gccgccgccg cgttcttcgc cggacacgcc acccaccacc acgcggacga ggacgaggac 960
gccggccaga tcagctggtg gatgtggaac tag 993
<210>8
<211>330
<212>PRT
＜213〉Zea mays
<400>8
Met Glu Ser Gly Arg Leu Ile Phe Asn Ala Pro Gly Ser Gly Ala Gly
1 5 10 15
Gln Met Leu Phe Leu Asp Cys Gly Ala Gly Gly Gly Pro Gly Gly Gly
20 25 30
Leu Phe His Arg Gly Gly Arg Pro Met Leu Gly Leu Glu Glu Gly Arg
35 40 45
Gly Val Lys Arg Pro Phe Phe Thr Ser Pro Asp Glu Leu Leu Glu Glu
50 55 60
Glu Tyr Tyr Asp Glu Gln Leu Pro Glu Lys Lys Arg Arg Leu Thr Pro
65 70 75 80
Glu Gln Val Leu Leu Leu Glu Arg Ser Phe Glu Glu Glu Asn Lys Leu
85 90 95
Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg Lys Leu Gly Leu Gln Pro
100 105 110
Arg Gln Val Ala Val Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr
115 120 125
Lys Gln Leu Glu Arg Asp Phe Asp Arg Leu Lys Ala Ser Phe Asp Ala
130 135 140
Leu Arg Ala Asp His Asp Ala Leu Leu Gln Asp Asn Asn Arg Leu Arg
145 150 155 160
Ser Gln Val Val Ser Leu Thr Glu Lys Leu Gln Glu Lys Glu Asp Ala
165 170 175
Thr Glu Gly Gly Ala Thr Ala Asp Thr Ala Ala Pro Ala Val Asp Val
180 185 190
Glu Ala Ser Leu Ala Asp Asp Val Glu Glu Pro Ala Glu Pro Ala Ala
195 200 205
Thr Phe Glu Val Leu Gln Glu Val Lys Ser Glu Asp Arg Leu Ser Thr
210 215 220
Gly Ser Gly Gly Ser Ala Val Val Asp Ala Asp Ala Leu Leu Tyr Gly
225 230 235 240
Arg Phe Ala Ala Ala Val Asp Ser Ser Val Glu Ser Tyr Phe Pro Gly
245 250 255
Gly Glu Asp His Tyr His Asp Cys Gly Thr Met Gly Pro Val Asn His
260 265 270
Gly Ala Gly Gly Gly Ile Gln Ser Asp Asp Asp Gly Ala Gly Ser Asp
275 280 285
Glu Gly Cys Ser Tyr Tyr Ala Asp Glu Ala Ala Ala Ala Ala Ala Ala
290 295 300
Phe Phe Ala Gly His Ala Thr His His His Ala Asp Glu Asp Glu Asp
305 310 315 320
Ala Gly Gln Ile Ser Trp Trp Met Trp Asn
325 330
<210>9
<211>825
<212>DNA
＜213〉sugarcane (Saccharum officinarum)
<400>9
atggatccga gcgcggtcag tttcaactcc ggcggcgcgc ggcggggcgg cggcggcacg 60
cagatgctgc tcttcggcgg cggaggcagc gccaacagca acggcttctt ccgaggtgtt 120
ccgatggcgg tcctgggcat ggacgacgcg acgcgcgtgg gcaagcggcc cttcttcacc 180
acacacgagg agctcctgga ggaggagtac tacgacgagc aggcgcccga gaagaagcgc 240
cgtctgacgg cggagcaggt gcagctgctg gagcggagct tcgaggaaga gaacaagctg 300
gagcccgagc gcaagaccga gctggctcgc cgcctcggga tggcgccccg ccaggtggcc 360
gtctggttcc agaaccgccg cgcgcgctgg aagaccaagc agctcgagac cgactatgac 420
cacctcaagg ctgcctacga cgcgctcgcc gccgaccacc agggcctcct ggccgacaac 480
gatagcctcc gggcacaggt ggtctcccta acagagaagc tgcaaggcaa ggagacatcc 540
ccgtcggcca ccactgctgc ccaagaggtc gaccagccag acgaacacac cgcggcgtca 600
ggcactgaga aactgctggc gcagcagctc aaggacgacc tccacagcag cggcgactgc 660
actggccatg gtgccctctc ctcagaggaa gaagatggtg gtgtggtcag tgacgagggc 720
agctttgatc tcccggatgc catgtttgct gccggggtca cccaccatgg cgccgacgcc 780
gaggaggcac agctggccaa ctggacatcc tggttctgga actga 825
<210>10
<211>274
<212>PRT
＜213〉sugarcane
<400>10
Met Asp Pro Ser Ala Val Ser Phe Asn Ser Gly Gly Ala Arg Arg Gly
1 5 10 15
Gly Gly Gly Thr Gln Met Leu Leu Phe Gly Gly Gly Gly Ser Ala Asn
20 25 30
Ser Asn Gly Phe Phe Arg Gly Val Pro Met Ala Val Leu Gly Met Asp
35 40 45
Asp Ala Thr Arg Val Gly Lys Arg Pro Phe Phe Thr Thr His Glu Glu
50 55 60
Leu Leu Glu Glu Glu Tyr Tyr Asp Glu Gln Ala Pro Glu Lys Lys Arg
65 70 75 80
Arg Leu Thr Ala Glu Gln Val Gln Leu Leu Glu Arg Ser Phe Glu Glu
85 90 95
Glu Asn Lys Leu Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg Arg Leu
100 105 110
Gly Met Ala Pro Arg Gln Val Ala Val Trp Phe Gln Asn Arg Arg Ala
115 120 125
Arg Trp Lys Thr Lys Gln Leu Glu Thr Asp Tyr Asp His Leu Lys Ala
130 135 140
Ala Tyr Asp Ala Leu Ala Ala Asp His Gln Gly Leu Leu Ala Asp Asn
145 150 155 160
Asp Ser Leu Arg Ala Gln Val Val Ser Leu Thr Glu Lys Leu Gln Gly
165 170 175
Lys Glu Thr Ser Pro Ser Ala Thr Thr Ala Ala Gln Glu Val Asp Gln
180 185 190
Pro Asp Glu His Thr Ala Ala Ser Gly Thr Glu Lys Leu Leu Ala Gln
195 200 205
Gln Leu Lys Asp Asp Leu His Ser Ser Gly Asp Cys Thr Gly His Gly
210 215 220
Ala Leu Ser Ser Glu Glu Glu Asp Gly Gly Val Val Ser Asp Glu Gly
225 230 235 240
Ser Phe Asp Leu Pro Asp Ala Met Phe Ala Ala Gly Val Thr His His
245 250 255
Gly Ala Asp Ala Glu Glu Ala Gln Leu Ala Asn Trp Thr Ser Trp Phe
260 265 270
Trp Asn
<210>11
<211>828
<212>DNA
＜213〉dichromatism chinese sorghum (Sorghum bicolor)
<400>11
atggatccga gcgcggtcag tttcgactcc ggcggcgcgc ggcggggcgg cggcggcggc 60
ggcgcgcaga tgctgctctt cggcggcgga ggcagcgcca acagcaacgg cttcttccga 120
ggtgttccga tggcggtcct gggcatggac gacgcgacgc gcgtgggcaa gcggcctttc 180
ttcaccacgc acgaggagct cctggaggag gagtactacg acgagcaggc gcccgagaag 240
aagcgccgtc tgacggcgga gcaggtgcag ctgctggagc ggagcttcga ggaagagaac 300
aagctggagc cggagcgcaa gaccgagctg gctcgccgcc tcgggatggc gcctcgccag 360
gtggccgtct ggttccagaa ccgccgcgcg cgctggaaga ctaagcagct cgagaccgac 420
tatgaccgcc tcaaggctgc ctacgacgcg ctcgccgccg accaccaggg cctcctggcc 480
gacaacgata gcctccgggc acaggtgatc tccctaacgg ataagctgca acgcaaggag 540
acatccccgt cggcgaccac tgctgcccaa gaggtcgacc agccagacga acacaccgct 600
gcgtcaggca ctgagaaact gctggtgcag cagctcaagg acgacctcca cagcagcggc 660
gacttcactg gccatggtgc cctctcttca gaggaagagg atggtggtgt ggtcagcgac 720
gagggctgca gctttgatct cccggatgcc atgttcgctg ccggggtcac ccaccatggc 780
gccgaggagg cgcagctggc caactggaca tcctggttct ggaactga 828
<210>12
<211>275
<212>PRT
＜213〉dichromatism chinese sorghum
<400>12
Met Asp Pro Ser Ala Val Ser Phe Asp Ser Gly Gly Ala Arg Arg Gly
1 5 10 15
Gly Gly Gly Gly Gly Ala Gln Met Leu Leu Phe Gly Gly Gly Gly Ser
20 25 30
Ala Asn Ser Asn Gly Phe Phe Arg Gly Val Pro Met Ala Val Leu Gly
35 40 45
Met Asp Asp Ala Thr Arg Val Gly Lys Arg Pro Phe Phe Thr Thr His
50 55 60
Glu Glu Leu Leu Glu Glu Glu Tyr Tyr Asp Glu Gln Ala Pro Glu Lys
65 70 75 80
Lys Arg Arg Leu Thr Ala Glu Gln Val Gln Leu Leu Glu Arg Ser Phe
85 90 95
Glu Glu Glu Asn Lys Leu Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg
100 105 110
Arg Leu Gly Met Ala Pro Arg Gln Val Ala Val Trp Phe Gln Asn Arg
115 120 125
Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Thr Asp Tyr Asp Arg Leu
130 135 140
Lys Ala Ala Tyr Asp Ala Leu Ala Ala Asp His Gln Gly Leu Leu Ala
145 150 155 160
Asp Asn Asp Ser Leu Arg Ala Gln Val Ile Ser Leu Thr Asp Lys Leu
165 170 175
Gln Arg Lys Glu Thr Ser Pro Ser Ala Thr Thr Ala Ala Gln Glu Val
180 185 190
Asp Gln Pro Asp Glu His Thr Ala Ala Ser Gly Thr Glu Lys Leu Leu
195 200 205
Val Gln Gln Leu Lys Asp Asp Leu His Ser Ser Gly Asp Phe Thr Gly
210 215 220
His Gly Ala Leu Ser Ser Glu Glu Glu Asp Gly Gly Val Val Ser Asp
225 230 235 240
Glu Gly Cys Ser Phe Asp Leu Pro Asp Ala Met Phe Ala Ala Gly Val
245 250 255
Thr His His Gly Ala Glu Glu Ala Gln Leu Ala Asn Trp Thr Ser Trp
260 265 270
Phe Trp Asn
275
<210>13
<211>1023
<212>DNA
＜213〉wheat (Triticum aestivum)
<400>13
atggagcccg gccggctcat cttcaacacg tcgggctccg gcaacggaca gatgctcttc 60
atggactgcg gcgcgggcgg catcgccggc gcggccggca tgttccatcg aggggtgaga 120
ccggtcctcg gcggcatgga agaagggcgc ggcgtgaagc ggcccttctt cacctcgccg 180
gatgacatgc tcgaggagga gtactacgac gagcagctcc cggagaagaa gcggcgcctc 240
accccggagc aggtccacct gctggagagg agcttcgagg aggagaacaa gctggagccg 300
gagaggaaga cggagctggc ccgcaagctc gggctgcagc cacgccaggt ggccgtctgg 360
ttccagaacc gccgcgcccg gtggaagaca aagacgctgg agcgcgactt cgaccgcctc 420
aaggcgtcct tcgacgccct ccgggccgac cacgacgccc tcctccagga caaccaccgg 480
ctccggtcac aggtggtaac gttgaccgag aagatgcaag ataaggaggc gccggaaggc 540
agcttcggtg cagccgccga cgcctcggag ccggagcagg cggcggcgga ggcgaaggct 600
tccttggccg acgccgagga gcaggccgcg gcagcggagg cgttcgaggt ggtgcagcag 660
cagctgcacg tgaaggacga ggagaggctg agcccgggga gcggcgggag cgcggtgctg 720
gacgcgaggg acgcgctgct cgggagcgga tgcggcctcg ccggcgtggt ggacagcagc 780
gtggactcgt actgcttccc ggggggcgcc ggcggcgacg agtaccacga gtgcgtggtg 840
ggccccgtgg cgggcggcat ccagtcggag gaggacgacg gcgcgggcag cgacgagggc 900
tgcagctact accccgacga cgccgccgtc ttcttcgccg ccgcgcaagg gcacggccac 960
catcgcacgg acgacgacga tcagcaggac gacggccaga tcagctactg gatgtggaac 1020
tag 1023
<210>14
<211>340
<212>PRT
＜213〉wheat
<400>14
Met Glu Pro Gly Arg Leu Ile Phe Asn Thr Ser Gly Ser Gly Asn Gly
1 5 10 15
Gln Met Leu Phe Met Asp Cys Gly Ala Gly Gly Ile Ala Gly Ala Ala
20 25 30
Gly Met Phe His Arg Gly Val Arg Pro Val Leu Gly Gly Met Glu Glu
35 40 45
Gly Arg Gly Val Lys Arg Pro Phe Phe Thr Ser Pro Asp Asp Met Leu
50 55 60
Glu Glu Glu Tyr Tyr Asp Glu Gln Leu Pro Glu Lys Lys Arg Arg Leu
65 70 75 80
Thr Pro Glu Gln Val His Leu Leu Glu Arg Ser Phe Glu Glu Glu Asn
85 90 95
Lys Leu Glu Pro Glu Arg Lys Thr Glu Leu Ala Arg Lys Leu Gly Leu
100 105 110
Gln Pro Arg Gln Val Ala Val Trp Phe Gln Asn Arg Arg Ala Arg Trp
115 120 125
Lys Thr Lys Thr Leu Glu Arg Asp Phe Asp Arg Leu Lys Ala Ser Phe
130 135 140
Asp Ala Leu Arg Ala Asp His Asp Ala Leu Leu Gln Asp Asn His Arg
145 150 155 160
Leu Arg Ser Gln Val Val Thr Leu Thr Glu Lys Met Gln Asp Lys Glu
165 170 175
Ala Pro Glu Gly Ser Phe Gly Ala Ala Ala Asp Ala Ser Glu Pro Glu
180 185 190
Gln Ala Ala Ala Glu Ala Lys Ala Ser Leu Ala Asp Ala Glu Glu Gln
195 200 205
Ala Ala Ala Ala Glu Ala Phe Glu Val Val Gln Gln Gln Leu His Val
210 215 220
Lys Asp Glu Glu Arg Leu Ser Pro Gly Ser Gly Gly Ser Ala Val Leu
225 230 235 240
Asp Ala Arg Asp Ala Leu Leu Gly Ser Gly Cys Gly Leu Ala Gly Val
245 250 255
Val Asp Ser Ser Val Asp Ser Tyr Cys Phe Pro Gly Gly Ala Gly Gly
260 265 270
Asp Glu Tyr His Glu Cys Val Val Gly Pro Val Ala Gly Gly Ile Gln
275 280 285
Ser Glu Glu Asp Asp Gly Ala Gly Ser Asp Glu Gly Cys Ser Tyr Tyr
290 295 300
Pro Asp Asp Ala Ala Val Phe Phe Ala Ala Ala Gln Gly His Gly His
305 310 315 320
His Arg Thr Asp Asp Asp Asp Gln Gln Asp Asp Gly Gln Ile Ser Tyr
325 330 335
Trp Met Trp Asn
340
<210>15
<211>819
<212>DNA
＜213〉Arabidopis thaliana (Arabidopsis thaliana)
<400>15
atggaatcca attcgttttt cttcgatcca tctgcttcac acggcaacag catgttcttc 60
cttgggaatc tcaatcccgt cgtccaagga ggaggagcaa gatcgatgat gaacatggag 120
gaaacttcga agcgaaggcc cttctttagc tcccctgagg atctctacga cgatgacttt 180
tacgacgacc agttgcctga aaagaagcgt cgcctcacta ccgaacaagt gcatctgctg 240
gagaaaagct tcgagacaga gaacaagcta gagcctgaac gcaagactca gcttgccaag 300
aagcttggtc tacagccaag gcaagtggct gtctggtttc agaatcgccg agctcgttgg 360
aaaacaaaac agcttgagag agactacgat cttctcaagt ccacttacga ccaacttctt 420
tctaactacg actccatcgt catggacaac gataagctca gatccgaggt tacttccctg 480
accgaaaagc ttcagggcaa acaagagaca gctaatgaac cacctggtca agtgcccgaa 540
ccaaaccaac ttgatccggt ttacattaat gcggcagcaa tcaaaaccga ggaccggtta 600
agttcaggga gcgttgggag cgcggtacta gacgacgacg cacctcaact actagacagc 660
tgtgactctt acttcccaag catcgtaccc atccaagaca acagcaacgc cagtgatcat 720
gacaatgacc ggagctgttt cgccgacgtc tttgtgccca ccacttcacc gtcgcacgat 780
catcacggtg aatcattggc tttctgggga tggccttag 819
<210>16
<211>272
<212>PRT
＜213〉Arabidopis thaliana
<400>16
Met Glu Ser Asn Ser Phe Phe Phe Asp Pro Ser Ala Ser His Gly Asn
1 5 10 15
Ser Met Phe Phe Leu Gly Asn Leu Asn Pro Val Val Gln Gly Gly Gly
20 25 30
Ala Arg Ser Met Met Asn Met Glu Glu Thr Ser Lys Arg Arg Pro Phe
35 40 45
Phe Ser Ser Pro Glu Asp Leu Tyr Asp Asp Asp Phe Tyr Asp Asp Gln
50 55 60
Leu Pro Glu Lys Lys Arg Arg Leu Thr Thr Glu Gln Val His Leu Leu
65 70 75 80
Glu Lys Ser Phe Glu Thr Glu Asn Lys Leu Glu Pro Glu Arg Lys Thr
85 90 95
Gln Leu Ala Lys Lys Leu Gly Leu Gln Pro Arg Gln Val Ala Val Trp
100 105 110
Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Arg Asp
115 120 125
Tyr Asp Leu Leu Lys Ser Thr Tyr Asp Gln Leu Leu Ser Asn Tyr Asp
130 135 140
Ser Ile Val Met Asp Asn Asp Lys Leu Arg Ser Glu Val Thr Ser Leu
145 150 155 160
Thr Glu Lys Leu Gln Gly Lys Gln Glu Thr Ala Asn Glu Pro Pro Gly
165 170 175
Gln Val Pro Glu Pro Asn Gln Leu Asp Pro Val Tyr Ile Asn Ala Ala
180 185 190
Ala Ile Lys Thr Glu Asp Arg Leu Ser Ser Gly Ser Val Gly Ser Ala
195 200 205
Val Leu Asp Asp Asp Ala Pro Gln Leu Leu Asp Ser Cys Asp Ser Tyr
210 215 220
Phe Pro Ser Ile Val Pro Ile Gln Asp Asn Ser Asn Ala Ser Asp His
225 230 235 240
Asp Asn Asp Arg Ser Cys Phe Ala Asp Val Phe Val Pro Thr Thr Ser
245 250 255
Pro Ser His Asp His His Gly Glu Ser Leu Ala Phe Trp Gly Trp Pro
260 265 270
<210>17
<211>969
<212>DNA
＜213〉Radix Dauci Sativae (Daucus carota)
<400>17
atggcgggtc ggagggtgtt ctatggggag ggagccaata cgacgtcggc tagcctgttg 60
tttcatagtc aaagacctga gcctttcttt ctttctgcac cttctccttc tctaattggt 120
tcaaaatcca tggttagctt tcaagatgct aagcgaaaaa atccctacga tgggttcttt 180
atgcggtcat atgatgaaga agaaattggg gatgaagaat atgatgaata ctttcagcag 240
cctgagaaga agaggaggct caaggctgat caaatccagt ttcttgagaa aagttttgag 300
actgataaca agcttgagcc tgaaagaaaa gttcagcttg caaaagaact cggcttgcag 360
ccaagacagg ttgcgatatg gtttcagaac cgtcgagcac ggtggaagac caaaacacta 420
gaaaaagatt atgatgtatt gcaaaatagc tacaacagcc tcaaggctga ctatgacaat 480
ctacttgccg agaaagaaaa acttaaagcc gaggttctcg acctgacaga caagctactt 540
ctcaaagaag ataaggggag caagacagta gtttttgata agcaaaaggt gtctgcagca 600
ttccaacaag aacgtgttag taatgacata tctgtgggtg aagtactcag taactcagtt 660
atggactgca agcaagaaga tcataactct gtgaaaagtg atgcagttga ttctgacagt 720
ccacactaca gtgatgaagt ctactccagt tttatggagc cagtggatcg ctcttatgtt 780
tttgaacctg ctcagtcgga tatatctcaa gatgaagaag atgacatggg gaacaactta 840
tttctcccat catatcatgt tttctcaaag actgaagacg gtagttactc cgaccagcct 900
tcgaactctt cgtactttgg cttcccagtt gaagatcata cgtttggctt ttggggtact 960
gaattataa 969
<210>18
<211>322
<212>PRT
＜213〉Radix Dauci Sativae
<400>18
Met Ala Gly Arg Arg Val Phe Tyr Gly Glu Gly Ala Asn Thr Thr Ser
1 5 10 15
Ala Ser Leu Leu Phe His Ser Gln Arg Pro Glu Pro Phe Phe Leu Ser
20 25 30
Ala Pro Ser Pro Ser Leu Ile Gly Ser Lys Ser Met Val Ser Phe Gln
35 40 45
Asp Ala Lys Arg Lys Asn Pro Tyr Asp Gly Phe Phe Met Arg Ser Tyr
50 55 60
Asp Glu Glu Glu Ile Gly Asp Glu Glu Tyr Asp Glu Tyr Phe Gln Gln
65 70 75 80
Pro Glu Lys Lys Arg Arg Leu Lys Ala Asp Gln Ile Gln Phe Leu Glu
85 90 95
Lys Ser Phe Glu Thr Asp Asn Lys Leu Glu Pro Glu Arg Lys Val Gln
100 105 110
Leu Ala Lys Glu Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe
115 120 125
Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Thr Leu Glu Lys Asp Tyr
130 135 140
Asp Val Leu Gln Asn Ser Tyr Asn Ser Leu Lys Ala Asp Tyr Asp Asn
145 150 155 160
Leu Leu Ala Glu Lys Glu Lys Leu Lys Ala Glu Val Leu Asp Leu Thr
165 170 175
Asp Lys Leu Leu Leu Lys Glu Asp Lys Gly Ser Lys Thr Val Val Phe
180 185 190
Asp Lys Gln Lys Val Ser Ala Ala Phe Gln Gln Glu Arg Val Ser Asn
195 200 205
Asp Ile Ser Val Gly Glu Val Leu Ser Asn Ser Val Met Asp Cys Lys
210 215 220
Gln Glu Asp His Asn Ser Val Lys Ser Asp Ala Val Asp Ser Asp Ser
225 230 235 240
Pro His Tyr Ser Asp Glu Val Tyr Ser Ser Phe Met Glu Pro Val Asp
245 250 255
Arg Ser Tyr Val Phe Glu Pro Ala Gln Ser Asp Ile Ser Gln Asp Glu
260 265 270
Glu Asp Asp Met Gly Asn Asn Leu Phe Leu Pro Ser Tyr His Val Phe
275 280 285
Ser Lys Thr Glu Asp Gly Ser Tyr Ser Asp Gln Pro Ser Asn Ser Ser
290 295 300
Tyr Phe Gly Phe Pro Val Glu Asp His Thr Phe Gly Phe Trp Gly Thr
305 310 315 320
Glu Leu
<210>19
<211>1038
<212>DNA
＜213〉soybean (Glycine max)
<400>19
atggcgagtg gcaagcttta tgcgggttca aacatgtcac ttctcctcca aaacgaaagg 60
ctcccttgct cctctgaagt ccttgagtct ctttgggctc agacctctaa ccctgcttcc 120
ttccaaggtt caaaacccgt ggttgatttt gagaatgtaa gtgggagcag gatgacggat 180
aggcctttct ttcaagcgtt ggagaaggaa gagaactgtg atgaggatta cgaggggtgt 240
ttccaccaac cggggaagaa aaggaggctc acaagcgaac aagttcagtt ccttgaaagg 300
aactttgagg tagagaacaa gcttgaaccc gaaaggaaag tccaacttgc aaaagagctt 360
ggcttgcagc caaggcaagt tgctatatgg ttccaaaacc gaagggcaag gttcaagacc 420
aagcagctag aaaaagacta tggcgtgttg aaagctagtt atgacagact caaaagtgac 480
tatgaaagtc ttgttcaaga gaatgacaag ttaaaagcag aggtgaattc tctggagagc 540
aaattgattc ttagagataa agagaaggag gagaattcgg atgacaagtc atctcctgat 600
gatgctgtca attcttcttc accccacaac aacaaggagc ctatggattt attaattatt 660
tcaaaaaatg caacaacaac aacaacatct gaaaatggga ccaaagtgtt gtcaccactc 720
ccactcccta ttatggtaac atgctgcaag caagaagatg ccaactcagc caaaagtgat 780
gtccttgatt cggatagccc acattgcact tcattcgtgg agcctgctga ttcctctcat 840
gcctttgaac cagaagacca ctcagaagac ttctcccaag atgaagagga taaccttagt 900
gaaaaccttt tgatgacctt cccttcttct tgttgcttac ctaaggttga agaacactgc 960
tatgacggcc ctcctgaaaa ctcttgtaat tttggcttcc aggttgagga tcaaaccttc 1020
tgtttctggc cctattga 1038
<210>20
<211>345
<212>PRT
＜213〉soybean
<400>20
Met Ala Ser Gly Lys Leu Tyr Ala Gly Ser Asn Met Ser Leu Leu Leu
1 5 10 15
Gln Asn Glu Arg Leu Pro Cys Ser Ser Glu Val Leu Glu Ser Leu Trp
20 25 30
Ala Gln Thr Ser Asn Pro Ala Ser Phe Gln Gly Ser Lys Pro Val Val
35 40 45
Asp Phe Glu Asn Val Ser Gly Ser Arg Met Thr Asp Arg Pro Phe Phe
50 55 60
Gln Ala Leu Glu Lys Glu Glu Asn Cys Asp Glu Asp Tyr Glu Gly Cys
65 70 75 80
Phe His Gln Pro Gly Lys Lys Arg Arg Leu Thr Ser Glu Gln Val Gln
85 90 95
Phe Leu Glu Arg Asn Phe Glu Val Glu Asn Lys Leu Glu Pro Glu Arg
100 105 110
Lys Val Gln Leu Ala Lys Glu Leu Gly Leu Gln Pro Arg Gln Val Ala
115 120 125
Ile Trp Phe Gln Asn Arg Arg Ala Arg Phe Lys Thr Lys Gln Leu Glu
130 135 140
Lys Asp Tyr Gly Val Leu Lys Ala Ser Tyr Asp Arg Leu Lys Ser Asp
145 150 155 160
Tyr Glu Ser Leu Val Gln Glu Asn Asp Lys Leu Lys Ala Glu Val Asn
165 170 175
Ser Leu Glu Ser Lys Leu Ile Leu Arg Asp Lys Glu Lys Glu Glu Asn
180 185 190
Ser Asp Asp Lys Ser Ser Pro Asp Asp Ala Val Asn Ser Ser Ser Pro
195 200 205
His Asn Asn Lys Glu Pro Met Asp Leu Leu Ile Ile Ser Lys Ash Ala
210 215 220
Thr Thr Thr Thr Thr Ser Glu Asn Gly Thr Lys Val Leu Ser Pro Leu
225 230 235 240
Pro Leu Pro Ile Met Val Thr Cys Cys Lys Gln Glu Asp Ala Asn Ser
245 250 255
Ala Lys Ser Asp Val Leu Asp Ser Asp Ser Pro His Cys Thr Ser Phe
260 265 270
Val Glu Pro Ala Asp Ser Ser His Ala Phe Glu Pro Glu Asp His Ser
275 280 285
Glu Asp Phe Ser Gln Asp Glu Glu Asp Asn Leu Ser Glu Asn Leu Leu
290 295 300
Met Thr Phe Pro Ser Ser Cys Cys Leu Pro Lys Val Glu Glu His Cys
305 310 315 320
Tyr Asp Gly Pro Pro Glu Asn Ser Cys Asn Phe Gly Phe Gln Val Glu
325 330 335
Asp Gln Thr Phe Cys Phe Trp Pro Tyr
340 345
<210>21
<211>858
<212>DNA
<213>Craterostigma plantagineum
<400>21
atgaactctg ctcggatttt cttcgaccca tcttcccacg gcaacatgct gcagtttctt 60
gggaacgccg gcggcgattc atccgttttc cgaggaacaa gatcgtcgtc ggtgctgaac 120
atggaggaga gctcgttaaa acgacagatt ttcagcggcg gcggcggcga tgaattctac 180
gacgaggaat actacgacga gcagttgttg cctgagaaga agcgccgact caccgccgag 240
caggttcact tgcttgagaa gagcttcgag gctgagaaca agcttgagcc tgagcgaaag 300
gctgagctgg cgaagaagct cggattgcag ccgaggcaag tcgccatttg gttccaaaac 360
cgccgagcac ggtggaagac taagcagtta gagagggact acgacaagct taagtcttcc 420
tatgattctc ttctctcaac ctacgactct attcgccagg aaaacgacaa gctcaaagcc 480
gagctccttt ccctgaacga gaaattgcaa cccaaagacg acgacgaccc atcggccgaa 540
ataggtcgaa atctcagttc atcgtcgccg cctgtcgacg cggctgagcc gccgtgcctg 600
aagctgacgg tgaaggtgga ggaccgcctg agcacgggga gcaacggcag cgcagtaatg 660
gacggcgacg gacctcagca gctcctcgac gacagcggcg actcgtactt cgagaacgac 720
gaggaatacg actgcgccgc cgcaagtttg gctgctgcga aggaggacga cggcagcgat 780
gagggcgggt gttacttcac cgaggctctc gcggcggagg aggaggaggc gccgtttgct 840
tggtgtattt ggtcttaa 858
<210>22
<211>285
<212>PRT
<213>Craterostigma plantagineum
<400>22
Met Asn Ser Ala Arg Ile Phe Phe Asp Pro Ser Ser His Gly Asn Met
1 5 10 15
Leu Gln Phe Leu Gly Asn Ala Gly Gly Asp Ser Ser Val Phe Arg Gly
20 25 30
Thr Arg Ser Ser Ser Val Leu Asn Met Glu Glu Ser Ser Leu Lys Arg
35 40 45
Gln Ile Phe Ser Gly Gly Gly Gly Asp Glu Phe Tyr Asp Glu Glu Tyr
50 55 60
Tyr Asp Glu Gln Leu Leu Pro Glu Lys Lys Arg Arg Leu Thr Ala Glu
65 70 75 80
Gln Val His Leu Leu Glu Lys Ser Phe Glu Ala Glu Asn Lys Leu Glu
85 90 95
Pro Glu Arg Lys Ala Glu Leu Ala Lys Lys Leu Gly Leu Gln Pro Arg
100 105 110
Gln Val Ala Ile Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys
115 120 125
Gln Leu Glu Arg Asp Tyr Asp Lys Leu Lys Ser Ser Tyr Asp Ser Leu
130 135 140
Leu Ser Thr Tyr Asp Ser Ile Arg Gln Glu Asn Asp Lys Leu Lys Ala
145 150 155 160
Glu Leu Leu Ser Leu Asn Glu Lys Leu Gln Pro Lys Asp Asp Asp Asp
165 170 175
Pro Ser Ala Glu Ile Gly Arg Asn Leu Ser Ser Ser Ser Pro Pro Val
180 185 190
Asp Ala Ala Glu Pro Pro Cys Leu Lys Leu Thr Val Lys Val Glu Asp
195 200 205
Arg Leu Ser Thr Gly Ser Asn Gly Ser Ala Val Met Asp Gly Asp Gly
210 215 220
Pro Gln Gln Leu Leu Asp Asp Ser Gly Asp Ser Tyr Phe Glu Asn Asp
225 230 235 240
Glu Glu Tyr Asp Cys Ala Ala Ala Ser Leu Ala Ala Ala Lys Glu Asp
245 250 255
Asp Gly Ser Asp Glu Gly Gly Cys Tyr Phe Thr Glu Ala Leu Ala Ala
260 265 270
Glu Glu Glu Glu Ala Pro Phe Ala Trp Cys Ile Trp Ser
275 280 285
<210>23
<211>813
<212>DNA
＜213〉upland cotton (Gossypium hirsutum)
<220>
＜221〉variation
<222>(808)..(808)
＜223 〉/displacement=" c "/displacement=" g "/displacement=" t "
<400>23
atggagtctg gccgtctttt tttcaatccc tccactaccc accgcaacat gttgcttctc 60
gggaacactg aacccatctt tcgaggggca agaacaatgg ttagcatgga ggaaaaccca 120
aagaagcgac tgttcttcag ctcgccggag gatttgtacg acgaagagta ctacgacgag 180
cagttgcccg agaaaaagcg tcgccttacg tcggagcagg tgtatctgct agagaagagc 240
tttgaggcag agaacaagct ggagccggag aggaagagcc agttggccaa gaagttagga 300
ctgcaaccaa ggcaggtggc ggtatggttc cagaaccgcc gtgcaaggtg gaagacaaag 360
cagcttgaaa gggactatga cctcctcaaa tcttcctttg attcccttca gtccaattat 420
gacactattc tcaaagaaaa tgagaagctc aaatctgagg tagcttcctt gactgaaaaa 480
ctacaagcca aagatgtggc aacagaagca atagcaggtg aaaaggatga agggttagca 540
gctgagatgg cctccgccct ccaattcagt atgaaggtgg aggaccgtct tagtagcggc 600
agtgtcggaa gcgcggtggt ggatgaggat gccccacagc tggtggacag cggcaattcc 660
tactttccaa gcgatgaata ctccagaggc attggccctt tcgatggggt tcagtcggaa 720
gatgaggatg gcagtgataa ttgcgggagt tacttctccg atgtgttcgc aaccacagag 780
cagggagcat taggattgtg ggcctggatc taa 813
<210>24
<211>270
<212>PRT
＜213〉upland cotton
<220>
＜221〉uncertain
<222>(270)..(270)
＜223〉Xaa can be any naturally occurring amino acid
<400>24
Met Glu Ser Gly Arg Leu Phe Phe Asn Pro Ser Thr Thr His Arg Asn
1 5 10 15
Met Leu Leu Leu Gly Asn Thr Glu Pro Ile Phe Arg Gly Ala Arg Thr
20 25 30
Met Val Ser Met Glu Glu Asn Pro Lys Lys Arg Leu Phe Phe Ser Ser
35 40 45
Pro Glu Asp Leu Tyr Asp Glu Glu Tyr Tyr Asp Glu Gln Leu Pro Glu
50 55 60
Lys Lys Arg Arg Leu Thr Ser Glu Gln Val Tyr Leu Leu Glu Lys Ser
65 70 75 80
Phe Glu Ala Glu Asn Lys Leu Glu Pro Glu Arg Lys Ser Gln Leu Ala
85 90 95
Lys Lys Leu Gly Leu Gln Pro Arg Gln Val Ala Val Trp Phe Gln Asn
100 105 110
Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Arg Asp Tyr Asp Leu
115 120 125
Leu Lys Ser Ser Phe Asp Ser Leu Gln Ser Asn Tyr Asp Thr Ile Leu
130 135 140
Lys Glu Asn Glu Lys Leu Lys Ser Glu Val Ala Ser Leu Thr Glu Lys
145 150 155 160
Leu Gln Ala Lys Asp Val Ala Thr Glu Ala Ile Ala Gly Glu Lys Asp
165 170 175
Glu Gly Leu Ala Ala Glu Met Ala Ser Ala Leu Gln Phe Ser Met Lys
180 185 190
Val Glu Asp Arg Leu Ser Ser Gly Ser Val Gly Ser Ala Val Val Asp
195 200 205
Glu Asp Ala Pro Gln Leu Val Asp Ser Gly Asn Ser Tyr Phe Pro Ser
210 215 220
Asp Glu Tyr Ser Arg Gly Ile Gly Pro Phe Asp Gly Val Gln Ser Glu
225 230 235 240
Asp Glu Asp Gly Ser Asp Asn Cys Gly Ser Tyr Phe Ser Asp Val Phe
245 250 255
Ala Thr Thr Glu Gln Gly Ala Leu Gly Leu Trp Ala Trp Xaa
260 265 270
<210>25
<211>858
<212>DNA
＜213〉tomato (Lycopersicon esculentum)
<220>
＜221〉variation
<222>(294)..(294)
＜223 〉/displacement=" c "/displacement=" g "/displacement=" t "
<400>25
atgggatctg ggcatatatt tttcgacccg tcgtcgtgtc acggcaacat gctgttcctt 60
gggagcggag atcctgtttt ccgaggacca agatcgacga tgatgaagat ggaggactcc 120
tcgaagaggc gacccttctt tagctcgccg gaggatctat atgacgagga atactacgac 180
gagcagtcac cggagaagaa gcgccgtctc actcctgagc aggtgcactt gttggagaag 240
agctttgaga cagaaaacaa gctggagccc gagcgcaaaa cccagctggc ctaaaagctg 300
gggctgcagc ccagacaggt ggctgtatgg ttccaaaacc gccgtgcccg gtggaagacc 360
aagcagctcg agagggatta tgatcagctc aaatcctctt atgactccct tctctctgat 420
tttgactccg ttcgcaaaga taacgataag ctcaaatctg aggttgtttc attgatggaa 480
aagttacagg ggaaagtggt tggaggagca gggggaaatg aaaaatctga catcttggag 540
gtggatgcta tgacgatcct tcaagtgaag gtgaaggctg gggaccggtt gagcagtggc 600
agtggtggga gcgcggtggt agatgagcat agttcacagc tggtggacag tggggactca 660
tattttcaca ctgatcatga ggagtatcca gggcctggag gatgcaatgt tcctccaccc 720
atggatggtt tacaatcgga ggaagatgat ggtagtgatg atcatggcag ttgccatggc 780
tacttctcta acgtctttgt ggcagaagag cagcaccatg aacaaggaga agagcctatt 840
ggatggttct ggtcttaa 858
<210>26
<211>285
<212>PRT
＜213〉tomato
<220>
＜221〉uncertain
<222>(98)..(98)
＜223〉Xaa can be any naturally occurring amino acid
<400>26
Met Gly Ser Gly His Ile Phe Phe Asp Pro Ser Ser Cys His Gly Asn
1 5 10 15
Met Leu Phe Leu Gly Ser Gly Asp Pro Val Phe Arg Gly Pro Arg Ser
20 25 30
Thr Met Met Lys Met Glu Asp Ser Ser Lys Arg Arg Pro Phe Phe Ser
35 40 45
Ser Pro Glu Asp Leu Tyr Asp Glu Glu Tyr Tyr Asp Glu Gln Ser Pro
50 55 60
Glu Lys Lys Arg Arg Leu Thr Pro Glu Gln Val His Leu Leu Glu Lys
65 70 75 80
Ser Phe Glu Thr Glu Asn Lys Leu Glu Pro Glu Arg Lys Thr Gln Leu
85 90 95
Ala Xaa Lys Leu Gly Leu Gln Pro Arg Gln Val Ala Val Trp Phe Gln
100 105 110
Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Arg Asp Tyr Asp
115 120 125
Gln Leu Lys Ser Ser Tyr Asp Ser Leu Leu Ser Asp Phe Asp Ser Val
130 135 140
Arg Lys Asp Asn Asp Lys Leu Lys Ser Glu Val Val Ser Leu Met Glu
145 150 155 160
Lys Leu Gln Gly Lys Val Val Gly Gly Ala Gly Gly Asn Glu Lys Ser
165 170 175
Asp Ile Leu Glu Val Asp Ala Met Thr Ile Leu Gln Val Lys Val Lys
180 185 190
Ala Gly Asp Arg Leu Ser Ser Gly Ser Gly Gly Ser Ala Val Val Asp
195 200 205
Glu His Ser Ser Gln Leu Val Asp Ser Gly Asp Ser Tyr Phe His Thr
210 215 220
Asp His Glu Glu Tyr Pro Gly Pro Gly Gly Cys Asn Val Pro Pro Pro
225 230 235 240
Met Asp Gly Leu Gln Ser Glu Glu Asp Asp Gly Ser Asp Asp His Gly
245 250 255
Ser Cys His Gly Tyr Phe Ser Asn Val Phe Val Ala Glu Glu Gln His
260 265 270
His Glu Gln Gly Glu Glu Pro Ile Gly Trp Phe Trp Ser
275 280 285
<210>27
<211>972
<212>DNA
＜213〉tomato
<400>27
atggctccag ggattctcta tggtggttct tctaatttcg atggcgtttt tactcaaaaa 60
cagagagacg tgttttcttc atctactgca ccgaaagggc atcttggttc cctttttgcc 120
cctgcctctt cttcttctaa tttcttggga tccagttcta tggtgagttt tcgcggtgtt 180
aatggaggga agagatcatt ctttgattcg ttcgatcagg atgacaatga agctgatgaa 240
ttgggggaat atcttcatca agcggagaag aagaggcgac ttactgacaa ccaagttcag 300
tttcttgaga agagttttgg ggaagagaac aaacttgaac cagaaagaaa agttcagctt 360
gctaaagaac ttggtctgca gcctcgccaa attgcaattt ggtttcagaa tcgtcgtgcg 420
cgatggaaga ctaagcagct cgagaaagat tatgatgaat tgaggaatag atacgatact 480
ctgaaatcaa attacaataa tcttctcaag gaaaaagaag atcttcgaac tgaagttttc 540
cgtctcaccg gtaagctgtt tatcaaagag aaaggaaatg ggcaattgga tttgcgcgat 600
gaacacaaac actccaatgc attggcaaaa gaaaccgtgg ttgatccaat gtccaatgta 660
ccagctctgg ttgttaagca ccagcaggaa gatttaagct ctgctaagag tgatgttttc 720
gactcagaaa gcccacgtta caccagtaga atgcattcct cagtcgtaga tcaggatgat 780
tctgctcgcg catttgaaac tgatcagtcg gattcatctc aggatgatga tgaaaacttc 840
agcaagaata tgctttctac tgccaaccta cttggcaaag acgcggatga tgattatccc 900
gcgacatcat caaatttgag ttactttgga tttccagttg aagaccaagg ttttggtttc 960
tggacttatt aa 972
<210>28
<211>323
<212>PRT
＜213〉tomato
<400>28
Met Ala Pro Gly Ile Leu Tyr Gly Gly Ser Ser Asn Phe Asp Gly Val
1 5 10 15
Phe Thr Gln Lys Gln Arg Asp Val Phe Ser Ser Ser Thr Ala Pro Lys
20 25 30
Gly His Leu Gly Ser Leu Phe Ala Pro Ala Ser Ser Ser Ser Asn Phe
35 40 45
Leu Gly Ser Ser Ser Met Val Ser Phe Arg Gly Val Asn Gly Gly Lys
50 55 60
Arg Ser Phe Phe Asp Ser Phe Asp Gln Asp Asp Asn Glu Ala Asp Glu
65 70 75 80
Leu Gly Glu Tyr Leu His Gln Ala Glu Lys Lys Arg Arg Leu Thr Asp
85 90 95
Asn Gln Val Gln Phe Leu Glu Lys Ser Phe Gly Glu Glu Asn Lys Leu
100 105 110
Glu Pro Glu Arg Lys Val Gln Leu Ala Lys Glu Leu Gly Leu Gln Pro
115 120 125
Arg Gln Ile Ala Ile Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr
130 135 140
Lys Gln Leu Glu Lys Asp Tyr Asp Glu Leu Arg Asn Arg Tyr Asp Thr
145 150 155 160
Leu Lys Ser Asn Tyr Asn Asn Leu Leu Lys Glu Lys Glu Asp Leu Arg
165 170 175
Thr Glu Val Phe Arg Leu Thr Gly Lys Leu Phe Ile Lys Glu Lys Gly
180 185 190
Asn Gly Gln Leu Asp Leu Arg Asp Glu His Lys His Ser Asn Ala Leu
195 200 205
Ala Lys Glu Thr Val Val Asp Pro Met Ser Asn Val Pro Ala Leu Val
210 215 220
Val Lys His Gln Gln Glu Asp Leu Ser Ser Ala Lys Ser Asp Val Phe
225 230 235 240
Asp Ser Glu Ser Pro Arg Tyr Thr Ser Arg Met His Ser Ser Val Val
245 250 255
Asp Gln Asp Asp Ser Ala Arg Ala Phe Glu Thr Asp Gln Ser Asp Ser
260 265 270
Ser Gln Asp Asp Asp Glu Asn Phe Ser Lys Asn Met Leu Ser Thr Ala
275 280 285
Asn Leu Leu Gly Lys Asp Ala Asp Asp Asp Tyr Pro Ala Thr Ser Ser
290 295 300
Asn Leu Ser Tyr Phe Gly Phe Pro Val Glu Asp Gln Gly Phe Gly Phe
305 310 315 320
Trp Thr Tyr
<210>29
<211>1014
<212>DNA
＜213〉alfalfa (Medicago sativa)
<400>29
atggcgggtg ggagagtttt ttcaaatggt cctgcaaata tttcaaatat aaatatgaat 60
attttgcttc agaatcaaca acaaactcct cgtggaaact cttctcaaca acctcttgat 120
tctcttttcc tttcttcttc tgcttctttc tttggttcaa gatctatggt gagttttgaa 180
gatgttcaag gaaggaaaag gcgcaacagg tctttctttg gaggatttga tcttgacgaa 240
aacggagagg atgagatgga tgagtacttt catcaatccg agaagaaacg gcgtctctca 300
gtggatcaag ttcagtttct tgagaaaagc tttgaggagg acaacaaact tgaaccagag 360
aggaaaacca agctagctaa agaccttggt ttgcagccac ggcaagttgc tatttggttt 420
caaaaccgtc gtgcaaggtg gaagactaaa cagcttgaga aggattatga ttctcttaat 480
gatggttatg agtctcttaa gacagagtat gacaaccttc tcaaagagaa agataggtta 540
caatctgagg tggcaagcct aactgaaaag gtacttgaaa gagagaaaca agagggaaaa 600
ttcaaacaag gtgaaagtga aacaaaggaa ttcttgaagg aaccaacaat taataagcct 660
ttggttgatt cagtttctga gggtgaagga tccaaattgt caattgttga ggcttctaat 720
aataataata ataataacaa acttgaagat attagttcag caaggagtga catattggat 780
tgtgaaagtc cacgctacac tgatggagtg ttagagacat gtgattcttc ctatgtattt 840
gaacctgaat atcaatcgga cctatcacaa gatgaagaag atcacaattt attgcctcct 900
tacatcttta caaaacttga agatgtgaat tactccgacc cgccacataa ttcaacaagt 960
tatggatttc aagaggaaga tcatcatcaa gctctttggc cttggtctta ttag 1014
<210>30
<211>337
<212>PRT
＜213〉alfalfa
<400>30
Met Ala Gly Gly Arg Val Phe Ser Asn Gly Pro Ala Asn Ile Ser Asn
1 5 10 15
Ile Asn Met Asn Ile Leu Leu Gln Asn Gln Gln Gln Thr Pro Arg Gly
20 25 30
Asn Ser Ser Gln Gln Pro Leu Asp Ser Leu Phe Leu Ser Ser Ser Ala
35 40 45
Ser Phe Phe Gly Ser Arg Ser Met Val Ser Phe Glu Asp Val Gln Gly
50 55 60
Arg Lys Arg Arg Asn Arg Ser Phe Phe Gly Gly Phe Asp Leu Asp Glu
65 70 75 80
Asn Gly Glu Asp Glu Met Asp Glu Tyr Phe His Gln Ser Glu Lys Lys
85 90 95
Arg Arg Leu Ser Val Asp Gln Val Gln Phe Leu Glu Lys Ser Phe Glu
100 105 110
Glu Asp Asn Lys Leu Glu Pro Glu Arg Lys Thr Lys Leu Ala Lys Asp
115 120 125
Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Arg Arg
130 135 140
Ala Arg Trp Lys Thr Lys Gln Leu Glu Lys Asp Tyr Asp Ser Leu Asn
145 150 155 160
Asp Gly Tyr Glu Ser Leu Lys Thr Glu Tyr Asp Asn Leu Leu Lys Glu
165 170 175
Lys Asp Arg Leu Gln Ser Glu Val Ala Ser Leu Thr Glu Lys Val Leu
180 185 190
Glu Arg Glu Lys Gln Glu Gly Lys Phe Lys Gln Gly Glu Ser Glu Thr
195 200 205
Lys Glu Phe Leu Lys Glu Pro Thr Ile Asn Lys Pro Leu Val Asp Ser
210 215 220
Val Ser Glu Gly Glu Gly Ser Lys Leu Ser Ile Val Glu Ala Ser Asn
225 230 235 240
Asn Asn Asn Asn Asn Asn Lys Leu Glu Asp Ile Ser Ser Ala Arg Ser
245 250 255
Asp Ile Leu Asp Cys Glu Ser Pro Arg Tyr Thr Asp Gly Val Leu Glu
260 265 270
Thr Cys Asp Ser Ser Tyr Val Phe Glu Pro Glu Tyr Gln Ser Asp Leu
275 280 285
Ser Gln Asp Glu Glu Asp His Asn Leu Leu Pro Pro Tyr Ile Phe Thr
290 295 300
Lys Leu Glu Asp Val Asn Tyr Ser Asp Pro Pro His Asn Ser Thr Ser
305 310 315 320
Tyr Gly Phe Gln Glu Glu Asp His His Gln Ala Leu Trp Pro Trp Ser
325 330 335
Tyr
<210>31
<211>843
<212>DNA
<213>Aquilegia formosa x Aquilegia pubescens
<400>31
atggattcaa caacaagccg tcttttcttt gatggttcct gccatgggaa catgttgctt 60
ttagggagtg gagatcccgt tcttcgaggt tcaagatcat tcattaatat ggaagattct 120
ttgaaaagac gtccttttta tagttcaaca gatgaactaa ttgaagagga gttttatgat 180
gaacagctac ctgaaaagaa acgtcgtctt acttctgagc aggttcatct attggagaag 240
agctttgaga cagagaacaa gctggaacca gatcgtaaga cccagcttgc taagaagctt 300
gggttgcaac cgagacaagt tgcagtttgg tttcagaata gacgagctcg ttggaagact 360
aagcaactag agagagatta tgatcttctt aaagcttctt atgattccct tcgttctgat 420
tacgatgaca ttgttaaaga gaatgagaag ctcaaatctg aggtggtttc cttaactggg 480
aagttgcagg tcaaggaggg agctgggatg gagttaaatc agatatctga cccaccactc 540
tccactgaag aaaatgttga tgtaactacg atgcaattta atgttaaggt tgaggatcgc 600
ttgagctctg gcagtggggt aagtgctgtg gttgatgagg aatgtcgaca gcttgttgac 660
agtgttgatt cctatttccc tggcgatgac tatggtcaat gcataggccc agtagatgga 720
gtccagtcag aagaagatga cattagtgac gacagccgga gctatttctc agatgtcttt 780
ccagctgcac cagagcagaa ccaccaggag agtgagacat tgggttggtg ggactgggct 840
taa 843
<210>32
<211>280
<212>PRT
<213>Aquilegia formosa x Aquilegia pubescens
<400>32
Met Asp Ser Thr Thr Ser Arg Leu Phe Phe Asp Gly Ser Cys His Gly
1 5 10 15
Asn Met Leu Leu Leu Gly Ser Gly Asp Pro Val Leu Arg Gly Ser Arg
20 25 30
Ser Phe Ile Asn Met Glu Asp Ser Leu Lys Arg Arg Pro Phe Tyr Ser
35 40 45
Ser Thr Asp Glu Leu Ile Glu Glu Glu Phe Tyr Asp Glu Gln Leu Pro
50 55 60
Glu Lys Lys Arg Arg Leu Thr Ser Glu Gln Val His Leu Leu Glu Lys
65 70 75 80
Ser Phe Glu Thr Glu Asn Lys Leu Glu Pro Asp Arg Lys Thr Gln Leu
85 90 95
Ala Lys Lys Leu Gly Leu Gln Pro Arg Gln Val Ala Val Trp Phe Gln
100 105 110
Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Arg Asp Tyr Asp
115 120 125
Leu Leu Lys Ala Ser Tyr Asp Ser Leu Arg Ser Asp Tyr Asp Asp Ile
130 135 140
Val Lys Glu Asn Glu Lys Leu Lys Ser Glu Val Val Ser Leu Thr Gly
145 150 155 160
Lys Leu Gln Val Lys Glu Gly Ala Gly Met Glu Leu Asn Gln Ile Ser
165 170 175
Asp Pro Pro Leu Ser Thr Glu Glu Asn Val Asp Val Thr Thr Met Gln
180 185 190
Phe Asn Val Lys Val Glu Asp Arg Leu Ser Ser Gly Ser Gly Val Ser
195 200 205
Ala Val Val Asp Glu Glu Cys Arg Gln Leu Val Asp Ser Val Asp Ser
210 215 220
Tyr Phe Pro Gly Asp Asp Tyr Gly Gln Cys Ile Gly Pro Val Asp Gly
225 230 235 240
Val Gln Ser Glu Glu Asp Asp Ile Ser Asp Asp Ser Arg Ser Tyr Phe
245 250 255
Ser Asp Val Phe Pro Ala Ala Pro Glu Gln Asn His Gln Glu Ser Glu
260 265 270
Thr Leu Gly Trp Trp Asp Trp Ala
275 280
<210>33
<211>2193
<212>DNA
＜213〉rice
<400>33
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcasagaa 960
aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140
cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200
tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260
gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320
ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380
gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440
aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500
ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560
atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620
acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680
cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740
ctttctggtt cagttcaatg aattgattgc tacaaataat gcttttatag cgttatccta 1800
gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860
tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920
attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980
tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040
cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100
agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160
tggtgtagct tgccactttc accagcaaag ttc 2193
<210>34
<211>49
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm6000
<400>34
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga tcccggccg 49
<210>35
<211>48
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm6001
<400>35
ggggaccact ttgtacaaga aagctgggtg atcagctcca gaaccagg 48
<210>36
<211>834
<212>DNA
＜213〉rice
<400>36
atgaagcgac ccggcggtgc cggcggcggc ggaggcagcc catcgctcgt cacgatggct 60
aattctagtg atgatggata tggaggggtt gggatggagg cggaggggga cgtggaggag 120
gagatgatgg cgtgcggcgg cggcggggag aagaagcggc ggctgagcgt ggagcaggtt 180
cgcgcgctgg agcggagctt cgaggtggag aacaagcttg agcctgagcg gaaggcgcgg 240
ctggcgcgcg acctcggcct gcagccgcgc caggtcgccg tctggttcca gaaccgccgc 300
gcgcggtgga agaccaagca gctcgagcgc gactacgccg cgctccgcca ttcctacgac 360
tccctgcgcc tcgatcacga cgcgctccgc cgcgacaagg acgccctcct cgccgagatc 420
aaggagctga aggcgaagct cggggacgag gaggcggcgg cgagcttcac gtcggtgaag 480
gaggagccgg cggcctccga cgggccaccg gcggcgggat ttgggtcgtc cgacagcgac 540
tcaagcgcgg tgctgaacga cgtggacgcg gccggcgccg cgcccgcggc gacggacgcg 600
ctggctccgg aggcgtgcac gtttctcggt gcgccgcccg ccgcgggcgc gggcgcgggc 660
gcagcggcgg cggcgagcca cgaggaggtg ttcttccacg gcaatttcct caaggtggag 720
gaggacgaga cggggttcct cgacgacgac gagccgtgcg gcgggttctt cgccgacgat 780
cagcccccgc cgctctcgtc gtggtgggcc gaacccacgg agcactggaa ctga 834
<210>37
<211>277
<212>PRT
＜213〉rice
<400>37
Met Lys Arg Pro Gly Gly Ala Gly Gly Gly Gly Gly Ser Pro Ser Leu
1 5 10 15
Val Thr Met Ala Asn Ser Ser Asp Asp Gly Tyr Gly Gly Val Gly Met
20 25 30
Glu Ala Glu Gly Asp Val Glu Glu Glu Met Met Ala Cys Gly Gly Gly
35 40 45
Gly Glu Lys Lys Arg Arg Leu Ser Val Glu Gln Val Arg Ala Leu Glu
50 55 60
Arg Ser Phe Glu Val Glu Asn Lys Leu Glu Pro Glu Arg Lys Ala Arg
65 70 75 80
Leu Ala Arg Asp Leu Gly Leu Gln Pro Arg Gln Val Ala Val Trp Phe
85 90 95
Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Arg Asp Tyr
100 105 110
Ala Ala Leu Arg His Ser Tyr Asp Ser Leu Arg Leu Asp His Asp Ala
115 120 125
Leu Arg Arg Asp Lys Asp Ala Leu Leu Ala Glu Ile Lys Glu Leu Lys
130 135 140
Ala Lys Leu Gly Asp Glu Glu Ala Ala Ala Ser Phe Thr Ser Val Lys
145 150 155 160
Glu Glu Pro Ala Ala Ser Asp Gly Pro Pro Ala Ala Gly Phe Gly Ser
165 170 175
Ser Asp Ser Asp Ser Ser Ala Val Leu Asn Asp Val Asp Ala Ala Gly
180 185 190
Ala Ala Pro Ala Ala Thr Asp Ala Leu Ala Pro Glu Ala Cys Thr Phe
195 200 205
Leu Gly Ala Pro Pro Ala Ala Gly Ala Gly Ala Gly Ala Ala Ala Ala
210 215 220
Ala Ser His Glu Glu Val Phe Phe His Gly Asn Phe Leu Lys Val Glu
225 230 235 240
Glu Asp Glu Thr Gly Phe Leu Asp Asp Asp Glu Pro Cys Gly Gly Phe
245 250 255
Phe Ala Asp Asp Gln Pro Pro Pro Leu Ser Ser Trp Trp Ala Glu Pro
260 265 270
Thr Glu His Trp Asn
275
<210>38
<211>750
<212>DNA
＜213〉rice
<400>38
atggatgggg aggaggacag cgagtggatg atgatggacg ttggagggaa gggcgggaag 60
ggcggcggcg gcggcggcgc ggcggacagg aagaagcggt tcagcgagga gcagatcaag 120
tcgctggagt ccatgttcgc gacgcagacc aagctggagc cgaggcagaa gctgcagctc 180
gccagggagc tcggcctgca gcctcgccag gtcgccatct ggttccagaa caagcgcgcg 240
cggtggaagt ccaagcagct cgagcgcgag tactccgccc tccgcgacga ctacgacgcc 300
ctcctctgca gctacgagtc cctcaagaag gagaagctcg ccctcatcaa gcagctggag 360
aagctggcgg agatgctgca ggagccacgg gggaagtacg gcgataatgc cggggacgac 420
gcgcggtcgg gcggcgtcgc cggcatgaag aaggaggagt tcgtcggcgc gggcggcgcc 480
gccacgctct actcgtcggc cgagggtggc gggacgacga cgacgacgac ggccaagttg 540
atgccccact tcggcagcga cgacgtcgac gcggggctct tcctccggcc gtcgtcgcag 600
catcatccgc cgccgccgca cgccggtgcc ggcttcacgt cctccgagcc ggccgccgac 660
caccagtcct tcaacttcca ctcgagctgg ccgtcgtcca cggagcagac ctgcagcagc 720
acgccatggt gggaattcga gagcgagtga 750
<210>39
<211>249
<212>PRT
＜213〉rice
<400>39
Met Asp Gly Glu Glu Asp Ser Glu Trp Met Met Met Asp Val Gly Gly
1 5 10 15
Lys Gly Gly Lys Gly Gly Gly Gly Gly Gly Ala Ala Asp Arg Lys Lys
20 25 30
Arg Phe Ser Glu Glu Gln Ile Lys Ser Leu Glu Ser Met Phe Ala Thr
35 40 45
Gln Thr Lys Leu Glu Pro Arg Gln Lys Leu Gln Leu Ala Arg Glu Leu
50 55 60
Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Lys Arg Ala
65 70 75 80
Arg Trp Lys Ser Lys Gln Leu Glu Arg Glu Tyr Ser Ala Leu Arg Asp
85 90 95
Asp Tyr Asp Ala Leu Leu Cys Ser Tyr Glu Ser Leu Lys Lys Glu Lys
100 105 110
Leu Ala Leu Ile Lys Gln Leu Glu Lys Leu Ala Glu Met Leu Gln Glu
115 120 125
Pro Arg Gly Lys Tyr Gly Asp Asn Ala Gly Asp Asp Ala Arg Ser Gly
130 135 140
Gly Val Ala Gly Met Lys Lys Glu Glu Phe Val Gly Ala Gly Gly Ala
145 150 155 160
Ala Thr Leu Tyr Ser Ser Ala Glu Gly Gly Gly Thr Thr Thr Thr Thr
165 170 175
Thr Ala Lys Leu Met Pro His Phe Gly Ser Asp Asp Val Asp Ala Gly
180 185 190
Leu Phe Leu Arg Pro Ser Ser Gln His His Pro Pro Pro Pro His Ala
195 200 205
Gly Ala Gly Phe Thr Ser Ser Glu Pro Ala Ala Asp His Gln Ser Phe
210 215 220
Asn Phe His Ser Ser Trp Pro Ser Ser Thr Glu Gln Thr Cys Ser Ser
225 230 235 240
Thr Pro Trp Trp Glu Phe Glu Ser Glu
245
<210>40
<211>945
<212>DNA
＜213〉trembling poplar (Populus tremuloides)
<400>40
atggcgggtg gtaccggtgg ttctaattcc aatttgtctg ttttgcttca aagccaaaga 60
ggcccttgtg ctgcttcaca acctcttgaa tcttttttcc tttctggctc ttctccttct 120
tttcttggtt caagatccat gatgagtttt gaagatgttc atcaagcaaa cggatcaacc 180
aggccttttt tccgctcgtt tgatcacgaa gacaatggag acgatgatct ggatgaatat 240
tttcatcaac ctgaaaagaa gaggagactt actgttgatc aagttcagtt tcttgaaaag 300
agttttgagc ttgagaacaa gcttgaacct gaaaggaaaa tccagcttgc aaaggatctt 360
ggccttcagc cgcgtcaggt tgctatatgg tttcaaaacc gccgagcaag atggaagact 420
aaacagctgg aaaaggatta tgacgttttg caatctagct acaatagcct taaggctgac 480
tatgacaacc tcctcaagga gaaggagaaa ctaaaagctg aggttaatct tctcaccgac 540
aagttgctcc tcaaagagaa agagaaggga atctcagaat tgtctgataa agatgcatta 600
tcgcaagagc cacctaaaag ggctatagct gattcagctt ccgagggtga agtgtcgaaa 660
atctcaacag tggcctgtaa gcaggaagat atcagctcag ccaaaagcga catatttgat 720
tcagacagcc cacattacgc tgatggggtg cattcctcac tcttagaggc aggagattct 780
tcatatgttt tcgaacccga tcaatcagat ttgtcacaag atgaagaaga taactttagc 840
aagagcttat tgcctccata cgtctttccg aagcttgaag atgacgatta ctctgacccg 900
cctgcaagtt ttgaagatca tgccttttgg tcctggtcat actaa 945
<210>41
<211>314
<212>PRT
＜213〉trembling poplar
<400>41
Met Ala Gly Gly Thr Gly Gly Ser Asn Ser Asn Leu Ser Val Leu Leu
1 5 10 15
Gln Ser Gln Arg Gly Pro Cys Ala Ala Ser Gln Pro Leu Glu Ser Phe
20 25 30
Phe Leu Ser Gly Ser Ser Pro Ser Phe Leu Gly Ser Arg Ser Met Met
35 40 45
Ser Phe Glu Asp Val His Gln Ala Asn Gly Ser Thr Arg Pro Phe Phe
50 55 60
Arg Ser Phe Asp His Glu Asp Asn Gly Asp Asp Asp Leu Asp Glu Tyr
65 70 75 80
Phe His Gln Pro Glu Lys Lys Arg Arg Leu Thr Val Asp Gln Val Gln
85 90 95
Phe Leu Glu Lys Ser Phe Glu Leu Glu Asn Lys Leu Glu Pro Glu Arg
100 105 110
Lys Ile Gln Leu Ala Lys Asp Leu Gly Leu Gln Pro Arg Gln Val Ala
115 120 125
Ile Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu
130 135 140
Lys Asp Tyr Asp Val Leu Gln Ser Ser Tyr Asn Ser Leu Lys Ala Asp
145 150 155 160
Tyr Asp Asn Leu Leu Lys Glu Lys Glu Lys Leu Lys Ala Glu Val Asn
165 170 175
Leu Leu Thr Asp Lys Leu Leu Leu Lys Glu Lys Glu Lys Gly Ile Ser
180 185 190
Glu Leu Ser Asp Lys Asp Ala Leu Ser Gln Glu Pro Pro Lys Arg Ala
195 200 205
Ile Ala Asp Ser Ala Ser Glu Gly Glu Val Ser Lys Ile Ser Thr Val
210 215 220
Ala Cys Lys Gln Glu Asp Ile Ser Ser Ala Lys Ser Asp Ile Phe Asp
225 230 235 240
Ser Asp Ser Pro His Tyr Ala Asp Gly Val His Ser Ser Leu Leu Glu
245 250 255
Ala Gly Asp Ser Ser Tyr Val Phe Glu Pro Asp Gln Ser Asp Leu Ser
260 265 270
Gln Asp Glu Glu Asp Asn Phe Ser Lys Ser Leu Leu Pro Pro Tyr Val
275 280 285
Phe Pro Lys Leu Glu Asp Asp Asp Tyr Ser Asp Pro Pro Ala Ser Phe
290 295 300
Glu Asp His Ala Phe Trp Ser Trp Ser Tyr
305 310
<210>42
<211>954
<212>DNA
＜213〉trembling poplar
<400>42
atggcggctt gtggtggtgg tggtggtggt tctaatccca atttgtctgt tttagttcaa 60
agccaaagag gcccttgtgc tgcttctcaa cctcttgaag cttttttcct ttctggctct 120
tctccttctt ttcttggttc aagatccatg atgagttttg cagatgttca ccaagcaaat 180
ggatcaacta gaccgttttt ccgcccatat gatcacgaag acaacggcga cgatgatttg 240
gatgaatatt ttcatcaacc tgaaaagaag aggagactta ctgttgatca agttcagttt 300
cttgaaagaa gttttgaggt tgagaacaag cttgaacccg aaaggaaaat ccagctggcg 360
aaggatcttg gcttgcagcc tcggcaggtt gccatatggt ttcaaaaccg ccgggcaaga 420
tggaagacga aacagcttga aaaagattat gaggttctgc aatctagcta caatggcctt 480
aaggctgact acgacaacct cttcaaggag aaggagaaac taaaagctga ggttaatctt 540
ctcaccaacg agttgctcct taaagagaaa gagaaaggaa gctcagaatt gtctgataaa 600
gatgcattat ctcaagagcc acccaaaaag gcaatagccg attcagcttc agagggtgaa 660
gtgtcgaaaa cttcaaccgt ggcctgccag caggaagata ttagctcagc caaaagtgat 720
atgtttgatt cagacagccc acattttgcg gatggggtac attcctcact cttagaggca 780
ggtgattctt cacatgtctt cgagcccgac caatcggatt tatcacaaga tgaagaagat 840
aacttgagca agagtctttt gcctccgtac gtctttccaa agcttgaaga tggtgattac 900
tctgacccgc cagcaagttt tgaagatcat gccttttggt gctggtcata ctaa 954
<210>43
<211>317
<212>PRT
＜213〉trembling poplar
<400>43
Met Ala Ala Cys Gly Gly Gly Gly Gly Gly Ser Asn Pro Asn Leu Ser
1 5 10 15
Val Leu Val Gln Ser Gln Arg Gly Pro Cys Ala Ala Ser Gln Pro Leu
20 25 30
Glu Ala Phe Phe Leu Ser Gly Ser Ser Pro Ser Phe Leu Gly Ser Arg
35 40 45
Ser Met Met Ser Phe Ala Asp Val His Gln Ala Asn Gly Ser Thr Arg
50 55 60
Pro Phe Phe Arg Pro Tyr Asp His Glu Asp Asn Gly Asp Asp Asp Leu
65 70 75 80
Asp Glu Tyr Phe His Gln Pro Glu Lys Lys Arg Arg Leu Thr Val Asp
85 90 95
Gln Val Gln Phe Leu Glu Arg Ser Phe Glu Val Glu Asn Lys Leu Glu
100 105 110
Pro Glu Arg Lys Ile Gln Leu Ala Lys Asp Leu Gly Leu Gln Pro Arg
115 120 125
Gln Val Ala Ile Trp Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys
130 135 140
Gln Leu Glu Lys Asp Tyr Glu Val Leu Gln Ser Ser Tyr Asn Gly Leu
145 150 155 160
Lys Ala Asp Tyr Asp Asn Leu Phe Lys Glu Lys Glu Lys Leu Lys Ala
165 170 175
Glu Val Asn Leu Leu Thr Asn Glu Leu Leu Leu Lys Glu Lys Glu Lys
180 185 190
Gly Ser Ser Glu Leu Ser Asp Lys Asp Ala Leu Ser Gln Glu Pro Pro
195 200 205
Lys Lys Ala Ile Ala Asp Ser Ala Ser Glu Gly Glu Val Ser Lys Thr
210 215 220
Ser Thr Val Ala Cys Gln Gln Glu Asp Ile Ser Ser Ala Lys Ser Asp
225 230 235 240
Met Phe Asp Ser Asp Ser Pro His Phe Ala Asp Gly Val His Ser Ser
245 250 255
Leu Leu Glu Ala Gly Asp Ser Ser His Val Phe Glu Pro Asp Gln Ser
260 265 270
Asp Leu Ser Gln Asp Glu Glu Asp Asn Leu Ser Lys Ser Leu Leu Pro
275 280 285
Pro Tyr Val Phe Pro Lys Leu Glu Asp Gly Asp Tyr Ser Asp Pro Pro
290 295 300
Ala Ser Phe Glu Asp His Ala Phe Trp Cys Trp Ser Tyr
305 310 315
<210>44
<211>909
<212>DNA
＜213〉trembling poplar
<400>44
atggcgggtg ataaagactg tggcagttct aaaatgacca tttttcttcg aaacggcagg 60
ctccctcctt gtgaatctct ctgtattctc acctctttta gcactcttca tggtgcaaaa 120
tctatggtta attttaggaa tgatggagga gacactgtag acatgtcttt tttccaacca 180
catgtcaaag aagaaagtag cgatgaggat tatgatgcgc accttaagcc atctgaaaag 240
aaaaggcggc ttacagctgc tcaagtccag tttcttgaga agagctttga ggcggagaat 300
aagcttgaac cagagaggaa gatgcagctt gctaaagaac tcggcttgca gcctcgccag 360
gttgcaatat ggtttcaaaa ccgtagagct cggttcaaga acaagcagct ggaaagggac 420
tacgactcct tgagaatcag ctttgacaaa ctcaaggctg attatgacaa actcctcctc 480
gagaagcaga atttgaaaaa cgagcttctt tcactgaaag aaaaattgct tagcagagag 540
gaaagtatgg aaagttcaga accatttgat gtcatccatt caccggatgc agaacttgag 600
cctattcctg atacagtgtc tgaaaatgtt tccgccattg tgccaatggt gacacccaaa 660
caagaagaaa gttcagctaa aaatgatgtt ttcaactcag acagcccacg ttcatttttg 720
gagccccgtg attgttatcg tgttttcgag tcagaccaac cagatttttc ccaagttgaa 780
gaagataatc tcaccaggag ctttctaccc cctccgtact ttccaaaact ctaccgagag 840
ccacctgcaa gttcacgtaa ttttgaattc tcagcggaag atcagccctt ttggtcctgg 900
atttactga 909
<210>45
<211>302
<212>PRT
＜213〉trembling poplar
<400>45
Met Ala Gly Asp Lys Asp Cys Gly Ser Ser Lys Met Thr Ile Phe Leu
1 5 10 15
Arg Asn Gly Arg Leu Pro Pro Cys Glu Ser Leu Cys Ile Leu Thr Ser
20 25 30
Phe Ser Thr Leu His Gly Ala Lys Ser Met Val Asn Phe Arg Asn Asp
35 40 45
Gly Gly Asp Thr Val Asp Met Ser Phe Phe Gln Pro His Val Lys Glu
50 55 60
Glu Ser Ser Asp Glu Asp Tyr Asp Ala His Leu Lys Pro Ser Glu Lys
65 70 75 80
Lys Arg Arg Leu Thr Ala Ala Gln Val Gln Phe Leu Glu Lys Ser Phe
85 90 95
Glu Ala Glu Asn Lys Leu Glu Pro Glu Arg Lys Met Gln Leu Ala Lys
100 105 110
Glu Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp Phe Gln Asn Arg
115 120 125
Arg Ala Arg Phe Lys Asn Lys Gln Leu Glu Arg Asp Tyr Asp Ser Leu
130 135 140
Arg Ile Ser Phe Asp Lys Leu Lys Ala Asp Tyr Asp Lys Leu Leu Leu
145 150 155 160
Glu Lys Gln Asn Leu Lys Asn Glu Leu Leu Ser Leu Lys Glu Lys Leu
165 170 175
Leu Ser Arg Glu Glu Ser Met Glu Ser Ser Glu Pro Phe Asp Val Ile
180 185 190
His Ser Pro Asp Ala Glu Leu Glu Pro Ile Pro Asp Thr Val Ser Glu
195 200 205
Asn Val Ser Ala Ile Val Pro Met Val Thr Pro Lys Gln Glu Glu Ser
210 215 220
Ser Ala Lys Asn Asp Val Phe Asn Ser Asp Ser Pro Arg Ser Phe Leu
225 230 235 240
Glu Pro Arg Asp Cys Tyr Arg Val Phe Glu Ser Asp Gln Pro Asp Phe
245 250 255
Ser Gln Val Glu Glu Asp Asn Leu Thr Arg Ser Phe Leu Pro Pro Pro
260 265 270
Tyr Phe Pro Lys Leu Tyr Arg Glu Pro Pro Ala Ser Ser Arg Asn Phe
275 280 285
Glu Phe Ser Ala Glu Asp Gln Pro Phe Trp Ser Trp Ile Tyr
290 295 300
<210>46
<211>975
<212>DNA
＜213〉puncture vine clover (Medicago truncatula)
<400>46
atggcaggtg gcaagctttt tggtggttct aatatgtcac ttttgcttca aaatgaaaga 60
ctcccttgta cttctgaagt ccttgaatct ctttgggttc acacccctgc ttcttttcaa 120
ggttcaaatt cagtggttaa ttttgagaat ggtggtggta gcaacagagt ggtaacagat 180
agacccttct ttcaacaact tgagaaagaa gagaattgtg gtgatgaaga ttatgaagca 240
tgctaccatc aacaaggaaa gaaaaggagg ctttcaagtg aacaagttca atttcttgaa 300
aagagttttg aggtagaaaa caagcttgaa cctgatagga aagttcaact tgcaaaagag 360
cttggtttgc aaccaagaca agttgctata tggtttcaaa acagaagggc aaggttcaaa 420
actaaacagc ttgaaaaaga ttatggcaca ttgaaagcta gctttgatag tctcaaagat 480
gattatgata atcttcttca agagaatgac aagttaaaag aagaggtgaa ttctctcaag 540
aacaaattga tcccaagaga taaagaaaaa gtgaattcag aagacaaatc atcaccagaa 600
gcaatcaatt cacctcataa caacatagat ccaatggata taatttcaat tacaaattca 660
gaaaatgggt ccaaaatgtc actccctaat atggtactaa aatgtaagca agaagatgcc 720
aattcagcta aaagtgatgt gcttgattct gatagcccac attgcaatga tgggaacaat 780
ctttcttctt tcatagagcc tacagattca gatttctcac aagatgaaga ggataatgat 840
aacttgagtc ataatctttt gactcttcct tgcttaccaa aagttgaaga tgtttgctat 900
gatgacccac atgaaaattc ttgtaatttt gggttccctg ttgaagatca aaccttttgt 960
ttctggcctt attga 975
<210>47
<211>324
<212>PRT
＜213〉puncture vine clover
<400>47
Met Ala Gly Gly Lys Leu Phe Gly Gly Ser Asn Met Ser Leu Leu Leu
1 5 10 15
Gln Asn Glu Arg Leu Pro Cys Thr Ser Glu Val Leu Glu Ser Leu Trp
20 25 30
Val His Thr Pro Ala Ser Phe Gln Gly Ser Asn Ser Val Val Asn Phe
35 40 45
Glu Asn Gly Gly Gly Ser Asn Arg Val Val Thr Asp Arg Pro Phe Phe
50 55 60
Gln Gln Leu Glu Lys Glu Glu Asn Cys Gly Asp Glu Asp Tyr Glu Ala
65 70 75 80
Cys Tyr His Gln Gln Gly Lys Lys Arg Arg Leu Ser Ser Glu Gln Val
85 90 95
Gln Phe Leu Glu Lys Ser Phe Glu Val Glu Asn Lys Leu Glu Pro Asp
100 105 110
Arg Lys Val Gln Leu Ala Lys Glu Leu Gly Leu Gln Pro Arg Gln Val
115 120 125
Ala Ile Trp Phe Gln Asn Arg Arg Ala Arg Phe Lys Thr Lys Gln Leu
130 135 140
Glu Lys Asp Tyr Gly Thr Leu Lys Ala Ser Phe Asp Ser Leu Lys Asp
145 150 155 160
Asp Tyr Asp Asn Leu Leu Gln Glu Asn Asp Lys Leu Lys Glu Glu Val
165 170 175
Asn Ser Leu Lys Asn Lys Leu Ile Pro Arg Asp Lys Glu Lys Val Asn
180 185 190
Ser Glu Asp Lys Ser Ser Pro Glu Ala Ile Asn Ser Pro His Asn Asn
195 200 205
Ile Asp Pro Met Asp Ile Ile Ser Ile Thr Asn Ser Glu Asn Gly Ser
210 215 220
Lys Met Ser Leu Pro Asn Met Val Leu Lys Cys Lys Gln Glu Asp Ala
225 230 235 240
Asn Ser Ala Lys Ser Asp Val Leu Asp Ser Asp Ser Pro His Cys Asn
245 250 255
Asp Gly Asn Asn Leu Ser Ser Phe Ile Glu Pro Thr Asp Ser Asp Phe
260 265 270
Ser Gln Asp Glu Glu Asp Asn Asp Asn Leu Ser His Asn Leu Leu Thr
275 280 285
Leu Pro Cys Leu Pro Lys Val Glu Asp Val Cys Tyr Asp Asp Pro His
290 295 300
Glu Asn Ser Cys Asn Phe Gly Phe Pro Val Glu Asp Gln Thr Phe Cys
305 310 315 320
Phe Trp Pro Tyr
<210>48
<211>984
<212>DNA
＜213〉Kidney bean (Phaseolus vulgaris)
<400>48
atggcgggtg gcaagcttca tcctggttca aacatgtcac ttctcctcca aaacgacagg 60
ctcccttgct cctctgaagt ccttgagtct ctttgggctc acacctctaa cgctgcttcc 120
ttccaaggtt caaaatctat ggttgatttt gagaatgtta gtgggggcag ggtgacggat 180
aggccctttt ttcaagcgtt ggagaaggaa gataactgtg atgatgatta tgagggttgc 240
ttccatcaac cgggtaagaa aaggaggctc acaagcgaac aagttcagtt ccttgaaagg 300
aactttgagg tcgagaacaa gcttgaacct gaaaggaagg tccaacttgc aaaggagctt 360
ggcttgcagc caaggcaagt ggctatatgg ttccaaaacc gaagggcaag gttcaagacc 420
aagcagctag aaaaagatta tggcacattg aaagctagct atgacagact caaaggtgac 480
tatgaaagtc ttcttcaaga gaatgacaag ttaaaagcag aggtgaattc tctggagagc 540
aaattgattc ttagagataa agagaaggag aattcggacg acaagtcatc tcctgatgct 600
gtcaattcac cccacaaaga gcctatggat ttaatttcaa attcaacatc tgaaaatggg 660
accaaagtgt cactccctat tatggtaaca tgcaagcaag aagatgccaa ttcagccaaa 720
agtgatgtgc ttgattcgga cagcccacat tgcactgatg ggaaccatcc ctcttcattc 780
gtggagcctg ctgattcctc ccatgctttt gaaccagacc actccgactt ctcccaagat 840
gaagaggata atcttagtga aagccttttg accctccctt gcttaccaaa ggttgaagaa 900
gcctgctatg atgaccctcc tgaaaaccct tgtaattttg gcttccatgt cgaggatcaa 960
accttctgtt tctggcccta ttga 984
<210>49
<211>327
<212>PRT
＜213〉Kidney bean
<400>49
Met Ala Gly Gly Lys Leu His Pro Gly Ser Asn Met Ser Leu Leu Leu
1 5 10 15
Gln Asn Asp Arg Leu Pro Cys Ser Ser Glu Val Leu Glu Ser Leu Trp
20 25 30
Ala His Thr Ser Asn Ala Ala Ser Phe Gln Gly Ser Lys Ser Met Val
35 40 45
Asp Phe Glu Asn Val Ser Gly Gly Arg Val Thr Asp Arg Pro Phe Phe
50 55 60
Gln Ala Leu Glu Lys Glu Asp Asn Cys Asp Asp Asp Tyr Glu Gly Cys
65 70 75 80
Phe His Gln Pro Gly Lys Lys Arg Arg Leu Thr Ser Glu Gln Val Gln
85 90 95
Phe Leu Glu Arg Ash Phe Glu Val Glu Asn Lys Leu Glu Pro Glu Arg
100 105 110
Lys Val Gln Leu Ala Lys Glu Leu Gly Leu Gln Pro Arg Gln Val Ala
115 120 125
Ile Trp Phe Gln Asn Arg Arg Ala Arg Phe Lys Thr Lys Gln Leu Glu
130 135 140
Lys Asp Tyr Gly Thr Leu Lys Ala Ser Tyr Asp Arg Leu Lys Gly Asp
145 150 155 160
Tyr Glu Ser Leu Leu Gln Glu Asn Asp Lys Leu Lys Ala Glu Val Asn
165 170 175
Ser Leu Glu Ser Lys Leu Ile Leu Arg Asp Lys Glu Lys Glu Asn Ser
180 185 190
Asp Asp Lys Ser Ser Pro Asp Ala Val Asn Ser Pro His Lys Glu Pro
195 200 205
Met Asp Leu Ile Ser Asn Ser Thr Ser Glu Asn Gly Thr Lys Val Ser
210 215 220
Leu Pro Ile Met Val Thr Cys Lys Gln Glu Asp Ala Asn Ser Ala Lys
225 230 235 240
Ser Asp Val Leu Asp Ser Asp Ser Pro His Cys Thr Asp Gly Asn His
245 250 255
Pro Ser Ser Phe Val Glu Pro Ala Asp Ser Ser His Ala Phe Glu Pro
260 265 270
Asp His Ser Asp Phe Ser Gln Asp Glu Glu Asp Asn Leu Ser Glu Ser
275 280 285
Leu Leu Thr Leu Pro Cys Leu Pro Lys Val Glu G1u Ala Cys Tyr Asp
290 295 300
Asp Pro Pro Glu Asn Pro Cys Asn Phe Gly Phe His Val Glu Asp Gln
305 310 315 320
Thr Phe Cys Phe Trp Pro Tyr
325
<210>50
<211>957
<212>DNA
＜213〉Root or stem of Littleleaf Indianmulberry (Lotus corniculatus)
<400>50
atggcgggag ggagggtctt tagcggcggt tctgctgctc ctgcaaatgt ttccgatacc 60
agtcttttgc ttcagaatca acctcctgat tcttctctct tcctctctac ctctgcttct 120
tttctcggtt caagatccat ggtgagcttc gcagataata aattagggca aacgcggtcg 180
ttcttctccg cgtttgacct cgatgagaac ggcgatgagg tcatggacga gtactttcac 240
caatcggaga agaagcgccg tctctctgtt gaccaagttc agtttctgga gaagagcttc 300
gaggtggata acaagctcga acctgacagg aaaaccaaga ttgccaagga ccttggtttg 360
cagccacgcc aagtcgcaat ctggttccag aaccgccgtg cacggtggaa gacgaaacag 420
cttgagaagg attatgattc tctgcatagt agctttgaga gtctcaaatc caactatgat 480
aatcttctca aggagaaaga catgttaaaa gctgaggtgg caagtctcac tgagaaggtg 540
cttgcaagag agaatttgaa acaagttgaa agtgaaacaa agggattggt tgaaccaccc 600
caaaggcctt tacttgattc agtttcagag ggtgaagaat ctaaagtctc tgttggggct 660
tgtaaacatg aggatatcag ttcagccagg agtgagagtt tggattctga tagcccacgt 720
tacagggatg gatatggagt taactcagca gtgctagaga catgtgattc ttcttatgtg 780
gttgaacctg atcaatcgga tatgtcacag gatgaggaag acaacctgac caagaccctg 840
ttgcctccat acatgttttc caaacttgga gatatggatt actccgaccc gcctgaaagt 900
tcatgtaatt tcggatttcc ggaggaagat catgcccttt ggtcatggtc ttactga 957
<210>51
<211>318
<212>PRT
＜213〉Root or stem of Littleleaf Indianmulberry
<400>51
Met Ala Gly Gly Arg Val Phe Ser Gly Gly Ser Ala Ala Pro Ala Asn
1 5 10 15
Val Ser Asp Thr Ser Leu Leu Leu Gln Asn Gln Pro Pro Asp Ser Ser
20 25 30
Leu Phe Leu Ser Thr Ser Ala Ser Phe Leu Gly Ser Arg Ser Met Val
35 40 45
Ser Phe Ala Asp Asn Lys Leu Gly Gln Thr Arg Ser Phe Phe Ser Ala
50 55 60
Phe Asp Leu Asp Glu Asn Gly Asp Glu Val Met Asp Glu Tyr Phe His
65 70 75 80
Gln Ser Glu Lys Lys Arg Arg Leu Ser Val Asp Gln Val Gln Phe Leu
85 90 95
Glu Lys Ser Phe Glu Val Asp Asn Lys Leu Glu Pro Asp Arg Lys Thr
100 105 110
Lys Ile Ala Lys Asp Leu Gly Leu Gln Pro Arg Gln Val Ala Ile Trp
115 120 125
Phe Gln Asn Arg Arg Ala Arg Trp Lys Thr Lys Gln Leu Glu Lys Asp
130 135 140
Tyr Asp Ser Leu His Ser Ser Phe Glu Ser Leu Lys Ser Asn Tyr Asp
145 150 155 160
Asn Leu Leu Lys Glu Lys Asp Met Leu Lys Ala Glu Val Ala Ser Leu
165 170 175
Thr Glu Lys Val Leu Ala Arg Glu Asn Leu Lys Gln Val Glu Ser Glu
180 185 190
Thr Lys Gly Leu Val Glu Pro Pro Gln Arg Pro Leu Leu Asp Ser Val
195 200 205
Ser Glu Gly Glu Glu Ser Lys Val Ser Val Gly Ala Cys Lys His Glu
210 215 220
Asp Ile Ser Ser Ala Arg Ser Glu Ser Leu Asp Ser Asp Ser Pro Arg
225 230 235 240
Tyr Arg Asp Gly Tyr Gly Val Asn Ser Ala Val Leu Glu Thr Cys Asp
245 250 255
Ser Ser Tyr Val Val Glu Pro Asp Gln Ser Asp Met Ser Gln Asp Glu
260 265 270
Glu Asp Asn Leu Thr Lys Thr Leu Leu Pro Pro Tyr Met Phe Ser Lys
275 280 285
Leu Gly Asp Met Asp Tyr Ser Asp Pro Pro Glu Ser Ser Cys Asn Phe
290 295 300
Gly Phe Pro Glu Glu Asp His Ala Leu Trp Ser Trp Ser Tyr
305 310 315
<210>52
<211>1602
<212>DNA
＜213〉rice
<400>52
atggactcgt cgacggtggg cgctccgggg agctcgctgc acggcgtgac ggggcgcgag 60
ccggcgttcg cgttctcgac ggaggtgggc ggcgaggacg cggcggcggc gagcaagttc 120
gacttgccgg tggactcgga gcacaaggcg aagacgatca ggttgctgtc gttcgcgaac 180
ccgcatatga ggacgttcca cctatcatgg atctccttct tctcctgctt cgtctccacc 240
ttcgccgccg cccctctcgt ccccatcatc cgcgacaacc tcaacctcac caaggccgac 300
atcggcaacg ccggcgtcgc ctccgtctcc ggctccatct tctccaggct cgccatgggc 360
gccatctgcg acatgctcgg cccgcgctac ggctgcgcct tcctcatcat gctcgccgcg 420
cccaccgtct tctgcatgtc gctcatcgac tccgccgcgg ggtacatcgc cgtgcgcttc 480
ctcatcggct tctccctcgc caccttcgtg tcatgccagt actggatgag caccatgttc 540
aacagcaaga tcatcggcct cgtcaacggc ctcgccgccg ggtggggaaa catgggcggc 600
ggcgcgacgc agctcatcat gccgctcgtc tacgacgtga tccgcaagtg cggcgcgacg 660
ccgttcacgg cgtggaggct ggcctacttc gtgccgggga cgctgcacgt ggtgatgggc 720
gtgctggtgc tgacgctggg gcaggacctc cccgacggca acctgcgcag cctgcagaag 780
aagggtgacg tcaacaggga cagcttctcc agggtgctct ggtacgccgt caccaactac 840
cgcacctgga tcttcgtcct cctctacggc tactccatgg gcgtcgagct caccaccgac 900
aacgtcatcg ccgagtactt ctacgatcgc ttcgacctcg acctccgcgt cgccggcatc 960
atcgccgcat ccttcggcat ggccaacatc gtcgcgcgcc ccaccggcgg cctcctctcg 1020
gacctcggcg cgcgctactt cggcatgcgc gcccgcctct ggaacatttg gatcctccag 1080
accgccggcg gcgcgttctg cctcctgctc ggccgcgcat ccaccctccc cacctccgtc 1140
gtctgcatgg tcctcttctc cttctgcgcg caggccgcct gcggcgccat cttcggcgtc 1200
atccccttcg tctcccgccg ctcgctcggc atcatctccg gcatgaccgg cgccggcggc 1260
aacttcggcg ccgggctcac gcagctgctc ttcttcacgt cgtcgaggta ctccacgggc 1320
acggggctgg agtacatggg catcatgatc atggcgtgca cgctgccggt ggtgctcgtc 1380
catttcccgc agtggggctc catgttcctc ccgcccaacg ccggcgccga ggaggagcac 1440
tactacggct ccgagtggag cgaacaggag aagagcaagg gcctccacgg tgcaagtctc 1500
aagttcgccg agaactcccg ctccgagcgt ggccgccgca acgtcatcaa cgccgccgcc 1560
gccgccgcca cgccgcccaa caactcgccg gagcacgcct aa 1602
<210>53
<211>533
<212>PRT
＜213〉rice
<400>53
Met Asp Ser Ser Thr Val Gly Ala Pro Gly Ser Ser Leu His Gly Val
1 5 10 15
Thr Gly Arg Glu Pro Ala Phe Ala Phe Ser Thr Glu Val Gly Gly Glu
20 25 30
Asp Ala Ala Ala Ala Ser Lys Phe Asp Leu Pro Val Asp Ser Glu His
35 40 45
Lys Ala Lys Thr Ile Arg Leu Leu Ser Phe Ala Asn Pro His Met Arg
50 55 60
Thr Phe His Leu Ser Trp Ile Ser Phe Phe Ser Cys Phe Val Ser Thr
65 70 75 80
Phe Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu
85 90 95
Thr Lys Ala Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser
100 105 110
Ile Phe Ser Arg Leu Ala Met Gly Ala Ile Cys Asp Met Leu Gly Pro
115 120 125
Arg Tyr Gly Cys Ala Phe Leu Ile Met Leu Ala Ala Pro Thr Val Phe
130 135 140
Cys Met Ser Leu Ile Asp Ser Ala Ala Gly Tyr Ile Ala Val Arg Phe
145 150 155 160
Leu Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met
165 170 175
Ser Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Leu Ala
180 185 190
Ala Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro
195 200 205
Leu Val Tyr Asp Val Ile Arg Lys Cys Gly Ala Thr Pro Phe Thr Ala
210 215 220
Trp Arg Leu Ala Tyr Phe Val Pro Gly Thr Leu His Val Val Met Gly
225 230 235 240
Val Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg
245 250 255
Ser Leu Gln Lys Lys Gly Asp Val Asn Arg Asp Ser Phe Ser Arg Val
260 265 270
Leu Trp Tyr Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu
275 280 285
Tyr Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala
290 295 300
Glu Tyr Phe Tyr Asp Arg Phe Asp Leu Asp Leu Arg Val Ala Gly Ile
305 310 315 320
Ile Ala Ala Ser Phe Gly Met Ala Asn Ile Val Ala Arg Pro Thr Gly
325 330 335
Gly Leu Leu Ser Asp Leu Gly Ala Arg Tyr Phe Gly Met Arg Ala Arg
340 345 350
Leu Trp Asn Ile Trp Ile Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu
355 360 365
Leu Leu Gly Arg Ala Ser Thr Leu Pro Thr Ser Val Val Cys Met Val
370 375 380
Leu Phe Ser Phe Cys Ala Gln Ala Ala Cys Gly Ala Ile Phe Gly Val
385 390 395 400
Ile Pro Phe Val Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr
405 410 415
Gly Ala Gly Gly Asn Phe Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe
420 425 430
Thr Ser Ser Arg Tyr Ser Thr Gly Thr Gly Leu Glu Tyr Met Gly Ile
435 440 445
Met Ile Met Ala Cys Thr Leu Pro Val Val Leu Val His Phe Pro Gln
450 455 460
Trp Gly Ser Met Phe Leu Pro Pro Asn Ala Gly Ala Glu Glu Glu His
465 470 475 480
Tyr Tyr Gly Ser Glu Trp Ser Glu Gln Glu Lys Ser Lys Gly Leu His
485 490 495
Gly Ala Ser Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg Gly Arg
500 505 510
Arg Asn Val Ile Asn Ala Ala Ala Ala Ala Ala Thr Pro Pro Asn Asn
515 520 525
Ser Pro Glu His Ala
530
<210>54
<211>53
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm07061
<400>54
ggggacaagt ttgtacaaaa aagcaggctt aaacaatgga ctcgtcgacg gtg 53
<210>55
<211>50
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm07062
<400>55
ggggaccact ttgtacaaga aagctgggtc tcggtcgcag aattgtttac 50
<210>56
<211>3848
<212>DNA
＜213〉artificial sequence
<220>
＜223〉expression cassette
<400>56
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960
aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140
cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200
tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260
gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320
ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380
gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440
aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500
ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560
atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620
acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680
cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740
ctttctggtt cagttcaatg aattgattgc tacaaataat gcttttatag cgttatccta 1800
gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860
tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920
attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980
tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040
cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100
agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160
tggtgtagct tgccactttc accagcaaag ttcatttaaa tcaactaggg atatcacaag 2220
tttgtacaaa aaagcaggct taaacaatgg actcgtcgac ggtgggcgct ccggggagct 2280
cgctgcacgg cgtgacgggg cgcgagccgg cgttcgcgtt ctcgacggag gtgggcggcg 2340
aggacgcggc ggcggcgagc aagttcgact tgccggtgga ctcggagcac aaggcgaaga 2400
cgatcaggtt gctgtcgttc gcgaacccgc atatgaggac gttccaccta tcatggatct 2460
ccttcttctc ctgcttcgtc tccaccttcg ccgccgcccc tctcgtcccc atcatccgcg 2520
acaacctcaa cctcaccaag gccgacatcg gcaacgccgg cgtcgcctcc gtctccggct 2580
ccatcttctc caggctcgcc atgggcgcca tctgcgacat gctcggcccg cgctacggct 2640
gcgccttcct catcatgctc gccgcgccca ccgtcttctg catgtcgctc atcgactccg 2700
ccgcggggta catcgccgtg cgcttcctca tcggcttctc cctcgccacc ttcgtgtcat 2760
gccagtactg gatgagcacc atgttcaaca gcaagatcat cggcctcgtc aacggcctcg 2820
ccgccgggtg gggaaacatg ggcggcggcg cgacgcagct catcatgccg ctcgtctacg 2880
acgtgatccg caagtgcggc gcgacgccgt tcacggcgtg gaggctggcc tacttcgtgc 2940
cggggacgct gcacgtggtg atgggcgtgc tggtgctgac gctggggcag gacctccccg 3000
acggcaacct gcgcagcctg cagaagaagg gtgacgtcaa cagggacagc ttctccaggg 3060
tgctctggta cgccgtcacc aactaccgca cctggatctt cgtcctcctc tacggctact 3120
ccatgggcgt cgagctcacc accgacaacg tcatcgccga gtacttctac gatcgcttcg 3180
acctcgacct ccgcgtcgcc ggcatcatcg ccgcatcctt cggcatggcc aacatcgtcg 3240
cgcgccccac cggcggcctc ctctcggacc tcggcgcgcg ctacttcggc atgcgcgccc 3300
gcctctggaa catttggatc ctccagaccg ccggcggcgc gttctgcctc ctgctcggcc 3360
gcgcatccac cctccccacc tccgtcgtct gcatggtcct cttctccttc tgcgcgcagg 3420
ccgcctgcgg cgccatcttc ggcgtcatcc ccttcgtctc ccgccgctcg ctcggcatca 3480
tctccggcat gaccggcgcc ggcggcaact tcggcgccgg gctcacgcag ctgctcttct 3540
tcacgtcgtc gaggtactcc acgggcacgg ggctggagta catgggcatc atgatcatgg 3600
cgtgcacgct gccggtggtg ctcgtccatt tcccgcagtg gggctccatg ttcctcccgc 3660
ccaacgccgg cgccgaggag gagcactact acggctccga gtggagcgaa caggagaaga 3720
gcaagggcct ccacggtgca agtctcaagt tcgccgagaa ctcccgctcc gagcgtggcc 3780
gccgcaacgt catcaacgcc gccgccgccg ccgccacgcc gcccaacaac tcgccggagc 3840
acgcctaa 3848
<210>57
<211>27
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 1
<220>
＜221〉variant
<222>(1)..(1)
＜223 〉/displacement=" Ser "
<220>
＜221〉variant
<222>(2)..(2)
＜223 〉/displacement=" Pro "
<220>
＜221〉variant
<222>(3)..(3)
＜223 〉/displacement=" Gly "/displacement=" Ser "/displacement=" Ala "
<220>
＜221〉variant
<222>(5)..(5)
＜223 〉/displacement=" Val "/displacement=" Leu "
<220>
＜221〉variant
<222>(6)..(6)
＜223 〉/displacement=" Leu "/displacement=" Thr "
<220>
＜221〉variant
<222>(7)..(7)
＜223 〉/displacement=" Ala "/displacement=" Phe "/displacement=" Leu "
<220>
＜221〉variant
<222>(8)..(8)
＜223 〉/displacement=" Val "/displacement=" Met "/displacement=" Ile "
<220>
＜221〉variant
<222>(9)..(9)
＜223 〉/displacement=" Thr "/displacement=" Ile "/displacement=" Ala "/displacement=" Asn "
<220>
＜221〉variant
<222>(12)..(12)
＜223 〉/displacement=" Phe "
<220>
＜221〉variant
<222>(13)..(13)
＜223 〉/displacement=" Cys "/displacement=" Thr "
<220>
＜221〉variant
<222>(14)..(14)
＜223 〉/displacement=" Phe "/displacement=" Tyr "
<220>
＜221〉variant
<222>(16)..(16)
＜223 〉/displacement=" Ile "
<220>
＜221〉variant
<222>(19)..(19)
＜223 〉/displacement=" Ser "
<220>
＜221〉variant
<222>(20)..(20)
＜223 〉/displacement=" Ile "/displacement=" Val "
<220>
＜221〉variant
<222>(21)..(21)
＜223 〉/displacement=" Gly "/displacement=" Asn "
<220>
＜221〉variant
<222>(23)..(23)
＜223 〉/displacement=" Ile "/displacement=" Asn "
<220>
＜221〉variant
<222>(24)..(24)
＜223 〉/displacement=" Val "
<220>
＜221〉variant
<222>(25)..(25)
＜223 〉/displacement=" Ser "/displacement=" His "/displacement=" Val "
<220>
＜221〉variant
<222>(26)..(26)
＜223 〉/displacement=" Gln "/displacement=" Gly "
<400>57
Asn Tyr Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Ser Met Gly Val
1 5 10 15
Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr
20 25
<210>58
<211>10
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 2
<220>
＜221〉variant
<222>(3)..(3)
＜223 〉/displacement=" Ala "
<220>
＜221〉variant
<222>(7)..(7)
＜223 〉/displacement=" Thr "
<220>
＜221〉variant
<222>(10)..(10)
＜223 〉/displacement=" Ser "
<400>58
Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu
1 5 10
<210>59
<211>18
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 3
<220>
＜221〉variant
<222>(6)..(6)
＜223 〉/displacement=" Arg "
<220>
＜221〉variant
<222>(9)..(9)
＜223 〉/displacement=" Ile "
<220>
＜221〉variant
<222>(10)..(10)
＜223 〉/displacement=" Val "
<220>
＜221〉variant
<222>(11)..(11)
＜223 〉/displacement=" Leu "/displacement=" Val "
<220>
＜221〉variant
<222>(14)..(14)
＜223 〉/displacement=" Glu "
<220>
＜221〉variant
<222>(17)..(17)
＜223 〉/displacement=" Asp "
<220>
＜221〉variant
<222>(18)..(18)
＜223 〉/displacement=" Pro "
<400>59
Ser Thr Phe Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu
1 5 10 15
Asn Leu
<210>60
<211>10
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 4
<220>
＜221〉variant
<222>(4)..(4)
＜223 〉/displacement=" Met "
<220>
＜221〉variant
<222>(8)..(8)
＜223 〉/displacement=" Cys "
<400>60
Val Arg Phe Leu Ile Gly Phe Ser Leu Ala
1 5 10
<210>61
<211>12
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 5
<220>
＜221〉variant
<222>(5)..(5)
＜223 〉/displacement=" Arg "
<220>
＜221〉variant
<222>(8)..(8)
＜223 〉/displacement=" Thr "
<220>
＜221〉variant
<222>(10)..(10)
＜223 〉/displacement=" Val "
<220>
＜221〉variant
<222>(11)..(11)
＜223 〉/displacement=" Ser "
<220>
＜221〉variant
<222>(12)..(12)
＜223 〉/displacement=" Ser "
<400>61
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe
1 5 10
<210>62
<211>20
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 6
<220>
＜221〉variant
<222>(2)..(2)
＜223 〉/displacement=" Gln "/displacement=" Ser "/displacement=" Met "/displacement=" His "/displacement=" Thr "
<220>
＜221〉variant
<222>(4)..(4)
＜223 〉/displacement=" Val "
<220>
＜221〉variant
<222>(13)..(13)
＜223 〉/displacement=" Thr "
<220>
＜221〉variant
<222>(15)..(15)
＜223 〉/displacement=" Ala "
<220>
＜221〉variant
<222>(17)..(17)
＜223 〉/displacement=" Leu "
<220>
＜221〉variant
<222>(20)..(20)
＜223 〉/displacement=" Gly "
<400>62
Lys Ala Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
1 5 10 15
Phe Ser Arg Leu
20
<210>63
<211>8
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 7
<220>
＜221〉variant
<222>(3)..(3)
＜223 〉/displacement=" Thr "/displacement=" Cys "
<220>
＜221〉variant
<222>(5)..(5)
＜223 〉/displacement=" Gly "
<400>63
Asn Gly Leu Ala Ala Gly Trp Gly
1 5
<210>64
<211>13
<212>PRT
＜213〉artificial sequence
<220>
＜223〉sequence label 8
<220>
＜221〉variant
<222>(2)..(2)
＜223 〉/displacement=" Ser "
<220>
＜221〉variant
<222>(4)..(4)
＜223 〉/displacement=" Val "/displacement=" Gln "
<220>
＜221〉variant
<222>(7)..(7)
＜223 〉/displacement=" Pro "
<220>
＜221〉variant
<222>(8)..(8)
＜223 〉/displacement=" Val "/displacement=" I le "
<220>
＜221〉variant
<222>(9)..(9)
＜223 〉/displacement=" Glu "
<220>
＜221〉variant
<222>(11)..(11)
＜223 〉/displacement=" Ser "/displacement=" Asp "
<220>
＜221〉variant
<222>(12)..(12)
＜223 〉/displacement=" Thr "
<220>
＜221〉variant
<222>(13)..(13)
＜223 〉/displacement=" Ala "/displacement=" Thr "
<400>64
Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser
1 5 10
<210>65
<211>1858
<212>DNA
＜213〉reed (Phragmites australis)
<400>65
acaacagctc agatcaagca cctgcatttg cataagagct agcaagcaag caagtccaag 60
aaagaagcta gctagcttgt atcagatcaa gactcgagag gccagctatg gaggttggcg 120
cgccggggag ctcgctgcac ggcgtcacgg ggcgcgagcc ggcgttcgcc ttctcgacgt 180
ctgcggtgcc cgacgacgat gcagcgagca agttcgacct gccggtggac tcggagcaca 240
aggccaagag catccggctc ttctccttcg ccaacccgca catgcgcacc ttccatctct 300
cgtggatctc cttcttcacc tgcttcgtct ccaccttcgc cgccgcgccg ctcgtcccca 360
tcatccgcga caacctcaac ctcaccaagg ccgacatcgg caacgccggc gtggcctccg 420
tctcgggatc catcttctcc cgtctcgcca tgggcgccat ctgcgacctc ctcggcccgc 480
gctacggctg cgccttcctc atcatgctca cggcgcccac cgtcttctgc atgtccctca 540
tcgacgacgc agccggctac atcgtcgtca ggttcttgat cggcttctct ctggccacgt 600
tcgtgtcgtg ccagtactgg atgagcacca tgttcaatag caagatcatc gggacggtga 660
acgggctggc ggctgggtgg gggaacatgg gcggcggcgc cacgcagctc cccatgccgc 720
tcgtctacga cgtcatccgc aagtgcggcg cgacgccctt cacggcgtgg cgcatcgcct 780
acttcgtgcc ggggctaatg cacgtggtga tgggcatcct ggtgctcacg ctggggcagg 840
acctccccga cggcaacctg aggagcctcc agaagaaggg cgacgccaac aaggacaagt 900
tctccaaggt gctctggtac gccgtcacca actatcgcac ctggatcttc gtgctgctct 960
acggctactg catgggcgtg gagctgacca ccgacaacgt catcgccgag tactactacg 1020
accacttcga cctagacctc cgcgtcgccg gcatcatcgc cgcttgcttc ggaatggcca 1080
acatcgtggc acggcccttg ggcggcatcc tctccgacat cggcgcgcgc tactggggca 1140
tgcgcgcgcg cctctggaac atctggatcc tccagactgc tggcggcgcc ttctgcctct 1200
ggctcggccg cgccagcacg cttcctgcct ccattaccgc catggtgctc ttctccttct 1260
gcgcccaggc cgcctgcggc gccattttcg gcgtcacccc tttcgtcacc cgccgctccc 1320
tcggcatcat gtccgggatg acgggggctg gcggcaactt cggcgcgggg ctcacgcagc 1380
tgctcttctt cacctcgtcc aagtactcca cgggcatggg gctggagtac atgggcatca 1440
tgatcatggc gtgcacgctg cccgtggtgt tcgtgcactt cccgcaatgg ggatccatgc 1500
tcctcccgcc cagcgccggc gccgtcgagg agcactacta cagctcggag tggagtgagg 1560
aggagaagag caaggggctc cacagctcca gcctcaagtt ttcagagaac tgccgttcag 1620
agcgcggcaa ccgcaacgtc atcctcgcgg caccaaacag cacgcccgag cacgtataag 1680
acgcacgtat atgtccgcat tcgtacccct gtgcgcgtat acgcatacgc cagcggtttg 1740
tttaataagg tgcgcatata tatatatgta actagctata atgcgtatca ccgttatgcg 1800
cacgcacacg aaatatatat aattcgatgt tgacgcatat acaaaaaaaa aaaaaaaa 1858
<210>66
<211>523
<212>PRT
＜213〉reed
<400>66
Met Glu Val Gly Ala Pro Gly Ser Ser Leu His Gly Val Thr Gly Arg
1 5 10 15
Glu Pro Ala Phe Ala Phe Ser Thr Ser Ala Val Pro Asp Asp Asp Ala
20 25 30
Ala Ser Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser
35 40 45
Ile Arg Leu Phe Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu
50 55 60
Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala
65 70 75 80
Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr Lys Ala Asp
85 90 95
Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg
100 105 110
Leu Ala Met Gly Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys
115 120 125
Ala Phe Leu Ile Met Leu Thr Ala Pro Thr Val Phe Cys Met Ser Leu
130 135 140
Ile Asp Asp Ala Ala Gly Tyr Ile Val Val Arg Phe Leu Ile Gly Phe
145 150 155 160
Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe
165 170 175
Asn Ser Lys Ile Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly
180 185 190
Asn Met Gly Gly Gly Ala Thr Gln Leu Pro Met Pro Leu Val Tyr Asp
195 200 205
Val Ile Arg Lys Cys Gly Ala Thr Pro Phe Thr Ala Trp Arg Ile Ala
210 215 220
Tyr Phe Val Pro Gly Leu Met His Val Val Met Gly Ile Leu Val Leu
225 230 235 240
Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg Ser Leu Gln Lys
245 250 255
Lys Gly Asp Ala Asn Lys Asp Lys Phe Ser Lys Val Leu Trp Tyr Ala
260 265 270
Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys
275 280 285
Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Tyr
290 295 300
Asp His Phe Asp Leu Asp Leu Arg Val Ala Gly Ile Ile Ala Ala Cys
305 310 315 320
Phe Gly Met Ala Asn Ile Val Ala Arg Pro Leu Gly Gly Ile Leu Ser
325 330 335
Asp Ile Gly Ala Arg Tyr Trp Gly Met Arg Ala Arg Leu Trp Asn Ile
340 345 350
Trp Ile Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu Trp Leu Gly Arg
355 360 365
Ala Ser Thr Leu Pro Ala Ser Ile Thr Ala Met Val Leu Phe Ser Phe
370 375 380
Cys Ala Gln Ala Ala Cys Gly Ala Ile Phe Gly Val Thr Pro Phe Val
385 390 395 400
Thr Arg Arg Ser Leu Gly Ile Met Ser Gly Met Thr Gly Ala Gly Gly
405 410 415
Asn Phe Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Lys
420 425 430
Tyr Ser Thr Gly Met Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala
435 440 445
Cys Thr Leu Pro Val Val Phe Val His Phe Pro Gln Trp Gly Ser Met
450 455 460
Leu Leu Pro Pro Ser Ala Gly Ala Val Glu Glu His Tyr Tyr Ser Ser
465 470 475 480
Glu Trp Ser Glu Glu Glu Lys Ser Lys Gly Leu His Ser Ser Ser Leu
485 490 495
Lys Phe Ser Glu Asn Cys Arg Ser Glu Arg Gly Asn Arg Asn Val Ile
500 505 510
Leu Ala Ala Pro Asn Ser Thr Pro Glu His Val
515 520
<210>67
<211>1691
<212>DNA
＜213〉Zea mays
<400>67
ccaagggaaa gggatacatc acctgctgct ctagctctgc tatagcgcta gcagcaatat 60
aatggcggcc gtcggcgctc cggggagctc tctgcacgga gtcacggggc gcgagccggc 120
gttcgcattc tccacggagc acgaggaggc ggcgagcaat ggcggcaagt tcgacctgcc 180
ggtggactcg gagcacaagg cgaagagcgt ccggctcttc tccgtggcga acccgcacat 240
gcgcaccttc cacctctcct ggatctcctt cttcacctgc ttcgtgtcca ccttcgccgc 300
cgcgccgctg gtccccatca tccgcgacaa cctcaacctc accaaggccg acatcggcaa 360
cgcgggcgtg gcctccgtgt cgggctccat cttctcccgc ctcaccatgg gcgccgtctg 420
cgacctgctg ggcccgcgct acggctgcgc cttcctcatc atgctgtccg cgcccaccgt 480
gttctgcatg tcgctcatcg acgacgccgc gggctacatc accgtcaggt tcctcatcgg 540
cttctccctc gccaccttcg tctcctgcca gtactggatg agcaccatgt tcagcagcaa 600
gatcatcggc accgtcaacg ggctcgccgc cggatggggc aacatgggag gcggcgccac 660
gcagctcatc atgccgctcg tctacgacgt catccgcaag tgcggcgcca cgcccttcac 720
ggcgtggcgc ctcgcctact tcgtgccggg cctcatgcac gtcgtcatgg gcgtcctggt 780
gctcacgctg gggcaggacc tccccgacgg caacctcagg tcgctgcaga agaagggcaa 840
cgtcaacaag gacagcttct ccaaggtcat gtggtacgcc gtcatcaact accgcacctg 900
gatcttcgtc ctcctctacg gctactgcat gggcgtcgag ctcaccaccg acaacgtcat 960
cgccgagtac atgtacgacc gcttcgacct cgacctccgc gtcgccggga ccatcgccgc 1020
ctgcttcggc atggccaaca tcgtcgcgcg ccccatgggc ggcatcatgt ccgacatggg 1080
cgcgcgctac tggggcatgc gcgctcgcct ctggaacatc tggatcctcc agaccgccgg 1140
cggcgccttc tgcctctggc tgggacgcgc cagcaccctc cccgtctccg tcgtcgccat 1200
ggtgctcttc tccttctgcg cgcaggcggc ctgcggcgcc atcttcgggg tcatcccctt 1260
cgtctcccgc cgctccctcg gcatcatctc cggcatgacg ggcgccggcg gcaacttcgg 1320
cgcggggctc acgcagctgc tcttcttcac ctcctcaacc tactccacgg gcagggggct 1380
agagtacatg ggcatcatga tcatggcgtg cacgctacct gtggtgttcg tgcacttccc 1440
gcagtggggg tccatgttct tcccgcccag cgccaccgcc gacgaggagg gctactacgc 1500
ctccgagtgg aacgacgacg agaagagcaa gggactccat agcgccagcc tcaagtttgc 1560
cgagaacagc cgctcagagc gcggcaagcg aaacgtcatc caggccgatg ccgccgccac 1620
gccggagcat gtctaagtct actactaata agatggatcg atccatcatc catgttcacc 1680
tgctacctac c 1691
<210>68
<211>524
<212>PRT
＜213〉Zea mays
<400>68
Met Ala Ala Val Gly Ala Pro Gly Ser Ser Leu His Gly Val Thr Gly
1 5 10 15
Arg Glu Pro Ala Phe Ala Phe Ser Thr Glu His Glu Glu Ala Ala Ser
20 25 30
Asn Gly Gly Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys Ala Lys
35 40 45
Ser Val Arg Leu Phe Ser Val Ala Asn Pro His Met Arg Thr Phe His
50 55 60
Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala
65 70 75 80
Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr Lys Ala
85 90 95
Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser
100 105 110
Arg Leu Thr Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly
115 120 125
Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys Met Ser
130 135 140
Leu Ile Asp Asp Ala Ala Gly Tyr Ile Thr Val Arg Phe Leu Ile Gly
145 150 155 160
Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met
165 170 175
Phe Ser Ser Lys Ile Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp
180 185 190
Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu Val Tyr
195 200 205
Asp Val Ile Arg Lys Cys Gly Ala Thr Pro Phe Thr Ala Trp Arg Leu
210 215 220
Ala Tyr Phe Val Pro Gly Leu Met His Val Val Met Gly Val Leu Val
225 230 235 240
Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg Ser Leu Gln
245 250 255
Lys Lys Gly Asn Val Asn Lys Asp Ser Phe Ser Lys Val Met Trp Tyr
260 265 270
Ala Val Ile Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr
275 280 285
Cys Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Met
290 295 300
Tyr Asp Arg Phe Asp Leu Asp Leu Arg Val Ala Gly Thr Ile Ala Ala
305 310 315 320
Cys Phe Gly Met Ala Asn Ile Val Ala Arg Pro Met Gly Gly Ile Met
325 330 335
Ser Asp Met Gly Ala Arg Tyr Trp Gly Met Arg Ala Arg Leu Trp Asn
340 345 350
Ile Trp Ile Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu Trp Leu Gly
355 360 365
Arg Ala Ser Thr Leu Pro Val Ser Val Val Ala Met Val Leu Phe Ser
370 375 380
Phe Cys Ala Gln Ala Ala Cys Gly Ala Ile Phe Gly Val Ile Pro Phe
385 390 395 400
Val Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly Ala Gly
405 410 415
Gly Asn Phe Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser
420 425 430
Thr Tyr Ser Thr Gly Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met
435 440 445
Ala Cys Thr Leu Pro Val Val Phe Val His Phe Pro Gln Trp Gly Ser
450 455 460
Met Phe Phe Pro Pro Ser Ala Thr Ala Asp Glu Glu Gly Tyr Tyr Ala
465 470 475 480
Ser Glu Trp Asn Asp Asp Glu Lys Ser Lys Gly Leu His Ser Ala Ser
485 490 495
Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg Gly Lys Arg Asn Val
500 505 510
Ile Gln Ala Asp Ala Ala Ala Thr Pro Glu His Val
515 520
<210>69
<211>1789
<212>DNA
＜213〉Zea mays
<400>69
acgcggggaa gcacaagcaa ccagccagct agtttccaag ggatcacctg ctctctagca 60
ctagcagcaa tggcggccgt cggcgctccg ggcagctctc tgcacggagt cacggggcgc 120
gagccggcgt tcgccttctc cacggagcac gaggaggcgg cgagcaatgg tggcaagttc 180
gacctgccgg tggactcaga gcacaaggcg aagagcgtcc gtctcttctc cgtggcgaac 240
ccacacatgc gcaccttcca cctctcctgg atctccttct tcacctgctt cgtgtccacc 300
ttcgccgccg cgccgctggt ccccatcatc cgcgacaacc tcaacctcac caaggccgac 360
atcggcaacg cgggcgtggc ctcggtgtcg ggctccatct tctcccgcct caccatgggc 420
gccgtctgcg acctgctggg cccgcgctac ggctgcgcct tcctcatcat gctgtccgcg 480
cccaccgtgt tctgcatgtc gctcatcgac gacgccgcgg gctacatcac cgtcaggttc 540
ctcatcggct tctccctcgc caccttcgtc tcctgccagt actggatgag caccatgttc 600
agcagcaaga tcatcggcac cgtcaacggg ctcgccgccg gatggggcac aatgggaagg 660
cggcgccacg cagctcatat gccgctcgtc tacgacgtca tccgcaagtg cggcgccacg 720
ccattcacgg cctggcgcct cgcctacttc gtgccgggcc tcatgcacgt cgtcatgggc 780
gtcctggtgc tcacgctggg gcaggacctc cccgacggca acctcaggtc gctgcagaag 840
aagggcaacg tcaacaagga cagcttctcc aaggtcatgt ggtacgccgt catcaactac 900
cgtacctgga tctttgtcct cctctacggc tactgcatgg gcgtcgagct caccaccgac 960
aacgtcatcg ccgagtacat gtacgaccgc ttcgacctcg acctccgcgt cgctgggacc 1020
atcgccgcct gcttcggcat ggccaacatc gtcgcacgcc ccatgggcgg catcatgtcc 1080
gacatgggcg cgcgctactg gggcatgcgc gctcgcctct ggaacatctg gatcctccag 1140
accgccggcg gcgccttctg cctctggctg gggcgcgcca gcaccctccc cgtctccgtc 1200
gtcgccatgg tgctcttctc cttctgcgcg caggcggcat gcggcgccat cttcggggtt 1260
atcccctttg tctcccgccg ctccctcggc atcatctccg gcatgacggg cgccggcggc 1320
aacttcggcg ccgggctcac gcagctgctc ttctttacct cctcgaccta ctccacgggc 1380
agggggctgg agtacatggg catcatgatc atggcgtgca cgctgcccgt ggtgttcgtg 1440
cacttccctc agtgggggtc catgttcttt ccgcccagcg ccaccgccga cgaggagggc 1500
tactacgcct ccgagtggaa cgacgacgag aagagcaagg gactccatag cgccagcctc 1560
aagttcgccg agaacagccg ctcagagcgc ggcaagcgaa acgtcatcca ggccgacgcc 1620
gccgccacgc cggagcatgt ctaagtctac tactaagatg gatcgatcga cgatcaccta 1680
tacctctttg tatgtacgaa tatgccttgt tattactgcg cgcgcgcata tacaatacac 1740
gtgtgctccg ttgacatgag ttagaaaaaa aaaaaaaaaa aaaaaaaaa 1789
<210>70
<211>524
<212>PRT
＜213〉Zea mays
<400>70
Met Ala Ala Val Gly Ala Pro Gly Ser Ser Leu His Gly Val Thr Gly
1 5 10 15
Arg Glu Pro Ala Phe Ala Phe Ser Thr Glu His Glu Glu Ala Ala Ser
20 25 30
Asn Gly Gly Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys Ala Lys
35 40 45
Ser Val Arg Leu Phe Ser Val Ala Asn Pro His Met Arg Thr Phe His
50 55 60
Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala
65 70 75 80
Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr Lys Ala
85 90 95
Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser
100 105 110
Arg Leu Thr Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly
115 120 125
Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys Met Ser
130 135 140
Leu Ile Asp Asp Ala Ala Gly Tyr Ile Thr Val Arg Phe Leu Ile Gly
145 150 155 160
Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met
165 170 175
Phe Ser Ser Lys Ile Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp
180 185 190
Gly Thr Met Gly Arg Arg Arg His Ala Ala His Met Pro Leu Val Tyr
195 200 205
Asp Val Ile Arg Lys Cys Gly Ala Thr Pro Phe Thr Ala Trp Arg Leu
210 215 220
Ala Tyr Phe Val Pro Gly Leu Met His Val Val Met Gly Val Leu Val
225 230 235 240
Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg Ser Leu Gln
245 250 255
Lys Lys Gly Asn Val Asn Lys Asp Ser Phe Ser Lys Val Met Trp Tyr
260 265 270
Ala Val Ile Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr
275 280 285
Cys Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Met
290 295 300
Tyr Asp Arg Phe Asp Leu Asp Leu Arg Val Ala Gly Thr Ile Ala Ala
305 310 315 320
Cys Phe Gly Met Ala Asn Ile Val Ala Arg Pro Met Gly Gly Ile Met
325 330 335
Ser Asp Met Gly Ala Arg Tyr Trp Gly Met Arg Ala Arg Leu Trp Asn
340 345 350
Ile Trp Ile Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu Trp Leu Gly
355 360 365
Arg Ala Ser Thr Leu Pro Val Ser Val Val Ala Met Val Leu Phe Ser
370 375 380
Phe Cys Ala Gln Ala Ala Cys Gly Ala Ile Phe Gly Val Ile Pro Phe
385 390 395 400
Val Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly Ala Gly
405 410 415
Gly Asn Phe Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser
420 425 430
Thr Tyr Ser Thr Gly Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met
435 440 445
Ala Cys Thr Leu Pro Val Val Phe Val His Phe Pro Gln Trp Gly Ser
450 455 460
Met Phe Phe Pro Pro Ser Ala Thr Ala Asp Glu Glu Gly Tyr Tyr Ala
465 470 475 480
Ser Glu Trp Asn Asp Asp Glu Lys Ser Lys Gly Leu His Ser Ala Ser
485 490 495
Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg Gly Lys Arg Asn Val
500 505 510
Ile Gln Ala Asp Ala Ala Ala Thr Pro Glu His Val
515 520
<210>71
<211>1837
<212>DNA
＜213〉barley (Hordeum vulgare)
<400>71
gaattcgcgg ccgctccctt actacattgc aagccaagct caagagcagc agcaacagcc 60
accattagct gcttctagtt gttggcaaag atggaggtcg aggcgggcgc ccatggcgac 120
actgccgcga gcaagttcac gctgccggta gactccgagc acaaggccaa gtccttcagg 180
ctcttctcct tcgccaaccc gcacatgcgc accttccatc tctcgtggat ctccttcttc 240
acttgcttca tctccacctt cgccgcagcg ccccttgtcc ccatcattcg tgataacctc 300
aaccttgcca aggccgacat cggcaatgcc ggtgtggcat ccgtttctgg gtccatcttc 360
tccaggcttg ccatgggtgc catctgcgat ctcctcgggc cgcggtatgg atgtgcattc 420
ctcgtcatgc tctcggcacc gaccgttttc tgcatggccg ttatcgatga tgcctcaggg 480
tacatcgccg tccgctttct cattggcttc tcgcttgcta cgttcgtgtc atgccaatat 540
tggatgagca ccatgtttaa tagcaagatc atcggcacag tcaacggcct cgctgctgga 600
tggggcaaca tgggtggtgg cgccacgcag ctcatcatgc cgctcgtctt ccatgcaatc 660
cagaagtgtg gtgccacgcc cttcgtagcg tggcgtattg cctacttcgt gcccggaatg 720
atgcacatcg tgatgggctt gttggtactc accatggggc aagatctccc tgatgggaac 780
ctcgcaagtc tccagaagaa gggagacatg gccaaggaca agttctccaa ggtcctttgg 840
ggcgccgtta ccaactaccg aacatggatc tttgtcctcc tctatggcta ctgcatgggt 900
gtcgagctca ccaccgacaa tgtcattgcc gagtactact tcgaccactt ccacctagac 960
ctccgtgccg ccggtaccat cgctgcctgc ttcggcatgg ccaacatcgt cgcacgtcct 1020
acgggtggct acctctctga ccttggcgcc cgctatttcg gcatgcgtgc tcgcctctgg 1080
aatatctgga tcctccaaac cgctggtggc gctttctgca tctggctcgg tcgtgcatcg 1140
gccctccctg cctcggtgac cgccatggtc ctcttctcca tctgcgccca ggctgcgtgt 1200
ggtgctatct ttggtgtcgc acccttcgtc tccaggcgtt cccttggcat catttccggg 1260
ttgaccggtg ctggtggaaa cgtgggcgca gggctcacac agcttctctt cttcacgtca 1320
tcgcaatact ccactggtag gggtctcgag tacatgggca tcatgatcat ggcatgcacg 1380
ctgcccgtcg ctcttgtgca cttcccacaa tggggatcca tgttcttccc tgccagcgcc 1440
gacgccacgg aggaggagta ctacgcctcg gagtggtccg aagaggagaa agccaagggt 1500
ctccatatcg ccggccaaaa atttgctgag aattcccgct cggagcgcgg taggcgcaac 1560
gtcatccttg ccacgtccgc cacaccaccc aacaatacgc cccagcacgt atgagactgg 1620
attgtttttc atacctatgt acaagtactg aactacagtg cacgttcgta tatatatacg 1680
cctgcaacat cggctgtaat aaggcgtatg aatttacatt tgtagtgtag gcctgtgtaa 1740
tgcgtttctt acgcacgaaa tgtttggtct gtgcatgcac gcatgcgagg gtacctgtgc 1800
tctgaattta caacagcttt gaggcggccg cgaattc 1837
<210>72
<211>507
<212>PRT
＜213〉barley
<400>72
Met Glu Val Glu Ala Gly Ala His Gly Asp Thr Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Ile Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Val Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Thr
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Phe Asp His Phe His
275 280 285
Leu Asp Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Thr Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln
325 330 335
Thr Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu
340 345 350
Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala
355 360 365
Ala Cys Gly Ala Ile Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser
370 375 380
Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala
385 390 395 400
Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly
405 410 415
Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro
420 425 430
Val Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Phe Pro Ala
435 440 445
Ser Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu
450 455 460
Glu Glu Lys Ala Lys Gly Leu His Ile Ala Gly Gln Lys Phe Ala Glu
465 470 475 480
Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala Thr Ser
485 490 495
Ala Thr Pro Pro Asn Asn Thr Pro Gln His Val
500 505
<210>73
<211>1750
<212>DNA
＜213〉wheat
<220>
＜221〉variation
<222>(1201)..(1201)
＜223 〉/displacement=" c "/displacement=" g "/displacement=" t "
<400>73
tagttttgag tgcaactagg ctagctcaag aaagatggag gtgcaggccg gctctcatgc 60
cgacgccgcg gcgagcaagt tcacgctgcc ggtggactcc gagcacaagg ccaagtcctt 120
caggctcttc tccttcgcca acccccacat gcgcaccttt cacctctcgt ggatctcctt 180
cttcacctgc ttcgtctcca cctttgctgc ggcgcccctc gtgcccatca tccgcgacaa 240
cctcaacctt gccaaggctg acatcggcaa tgccggtgtc gcgtccgtgt ctgggtccat 300
cttctccagg ctggccatgg gcgctatctg tgacttgctt ggcccacggt atggttgtgc 360
cttcctcgtc atgctctcgg caccgaccgt cttctgcatg gccgtcatcg atgatgcctc 420
agggtacatc gccgtccggt tcctcattgg cttctccctc gccaccttcg tgtcatgcca 480
atactggatg agcaccatgt tcaatagcaa gatcattggc acggtcaatg gcttggctgc 540
aggctggggc aacatgggtg gcggcgccac gcagctcatc atgccgcttg tcttccacgc 600
aatccagaag tgtggcgcca cgcccttcgt ggcatggcgt attgcctact tcgtgccggg 660
aatgatgcac atcgtgatgg gcttgctggt cctcaccatg gggcaagatc tccctgacgg 720
gaaccttgcg agcctccara agaagggaga catggccaag gacaagttct ccaaggtcct 780
ttggggcgcy gtcaccaact accggacatg gatcttcgtc ctcctctacg gctactgcat 840
gggygtcgag ctcaccacsg acaatgtcat cgccgagtac tactacgacc acttccacct 900
agacctccgc gcygcwggca ccattgcygc ttgyttyggc atggcaaaca tygtcgcgcg 960
tcctatgggk ggctacctct cygaccttgg tgcccgctac tttggcatgc gtgctcgcct 1020
ttggaacatc tggatcctmc aaacygccgg tggcgctttc tgcatctggc tcggtcgtgc 1080
gtcagccctc cctgcctcag tgactgccat ggttctcttc tccatctgcg cccaagccgc 1140
gtgtggtgct gtatttggcg tcgcaccctt cgtttctaga cgttcccttg gcatcatctc 1200
agggctgacc ggtgctggtg gaaacgtggg tgcrgggctc acgcagcttc tcttctttac 1260
ttcatcacaa tactccacyg gaaggggtct cgagtacatg ggcatcatga tcatggcatg 1320
cacgctgcct gtcgctcttg tgcacttccc acagtggggc tcgatgttct tcccggccag 1380
cgccgacgcc acagaggagg aatactatgc ctctgagtgg tcggaggagg agaagaacaa 1440
gggtctccat attgctggcc aaaagtttgc cgagaactca cgatcggagc gtggaaggcg 1500
caacgtcatc cttgccacgt ccgccacgcc acccaacaat acgccccagc acgtataaga 1560
ccggattgtt tttcatatac tatgtacaag tactgaacat cggctgtaat aaggtgtacg 1620
catttatatt tccagtgtag acttgtgtaa tgcgtttctt acgcacgaaa tgttttggtg 1680
tgtgcatgca cgcatgcgag gtacctgtgg tctgaattta cagcaacttt gagactaaaa 1740
aaaaaaaaaa 1750
<210>74
<211>507
<212>PRT
＜213〉wheat
<400>74
Met Glu Val Gln Ala Gly Ser His Ala Asp Ala Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Val Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Thr
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Tyr Asp His Phe His
275 280 285
Leu Asp Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Met Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln
325 330 335
Thr Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu
340 345 350
Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala
355 360 365
Ala Cys Gly Ala Val Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser
370 375 380
Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala
385 390 395 400
Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly
405 410 415
Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro
420 425 430
Val Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Phe Pro Ala
435 440 445
Ser Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu
450 455 460
Glu Glu Lys Asn Lys Gly Leu His Ile Ala Gly Gln Lys Phe Ala Glu
465 470 475 480
Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala Thr Ser
485 490 495
Ala Thr Pro Pro Asn Asn Thr Pro Gln His Val
500 505
<210>75
<211>1741
<212>DNA
＜213〉barley
<400>75
ttacaagctc catctgagag cagcagcaac caccattaga gacacactta gttgccagtg 60
cgactaagct agctagctcg aggaagatgg aggtggagtc gagctcgcat ggcgccggcg 120
acgaggctgc gagcaagttc tcgctgcccg tggactcgga gcacaaggcc aagtccatca 180
ggctcttctc cttcgccaac ccccacatgc gcaccttcca cctctcctgg atctccttct 240
tcacctgctt cgtctccacc ttcgctgccg cgcccctcgt ccctatcatc cgcgacaacc 300
taaacctcgc caaggccgac atcggcaacg ccggtgtggc gtccgtgtcc gggtctatct 360
tctcgaggct cgccatgggg gccatctgcg atctccttgg ccctcgatat ggatgcgcct 420
tcctcgtcat gctcgcagca cccaccgtct tctgcatgtc cctcatcgat gatgcggcgg 480
gctacatcac ggtccgcttc ctcatcggct tctccctcgc gacgtttgtg tcgtgccagt 540
attggatgag caccatgttc aacagcaaga tcatcggcac cgtcaacggc ctggcggccg 600
gctggggcaa catgggtggt ggtgccaccc agctcattat gccactcgtc ttccacgcca 660
tccagaagtg tggtgccacg cccttcgtcg catggcgcat cgcctacttc gtgccaggaa 720
tgatgcacgt ggtgatgggc ttgctcgtgc tcaccatggg acaggatctc cccgatggta 780
accttgcaag cctccagaag aagggggaga tggccaagga caagttctcc aaggttgtgt 840
ggggtgctgt tacaaactac cgtacatgga tcttcgttct tctttacgga tactgcatgg 900
gtgttgagct caccaccgac aacgtcatcg ccgagtacta cttcgaccac tttcaccttg 960
accttcgaac atccggcacc attgccgcct gttttggcat ggccaacatc gttgctcggc 1020
ctgcgggtgg ctacctctcc gacctcggtg cccgctactt cggcatgcgt gcccgcctct 1080
ggaacatctg gatcctccag accgctggtg gcgcattctg cctctggctc ggccgtgcaa 1140
aagccctccc cgaatccatc actgccatgg tcctcttctc catctgcgct caggcagcat 1200
gtggtgcagt ctttggtgtc atccccttcg tctcccgccg ctccctcggc atcatttcgg 1260
gcttgagtgg agccggtggg aactttggcg ccgggctgac acaattgctc ttcttcactt 1320
cgtcgaagta tggcaccggc agggggcttg agtacatggg tatcatgatc atggcctgca 1380
cgctccctgt ggcgcttgtg cacttcccac agtggggttc catgctcttg ccgccaaacg 1440
ccaacgccac cgaggaggag ttctatgccg ccgaatggag cgaggaggag aagaagaagg 1500
gtctccatat ccctggccaa aagtttgccg agaattcccg ctcggagcgt ggcaggcgca 1560
acgtcatcct tgccacagcc gccacacccc ccaacaacac tccccaacac gcataagact 1620
cgagcttttc tttacctgtg tacacgtaca gtgcgcgtat tatacacaca tcgatcgtgt 1680
atatacgcct ggaatccgca agcagtatgt tttttgaaaa aaaaaaagcg gccgcgaatt 1740
c 1741
<210>76
<211>509
<212>PRT
＜213〉barley
<400>76
Met Glu Val Glu Ser Ser Ser His Gly Ala Gly Asp Glu Ala Ala Ser
1 5 10 15
Lys Phe Ser Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Ile Arg
20 25 30
Leu Phe Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp
35 40 45
Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu
50 55 60
Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly
65 70 75 80
Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala
85 90 95
Met Gly Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe
100 105 110
Leu Val Met Leu Ala Ala Pro Thr Val Phe Cys Met Ser Leu Ile Asp
115 120 125
Asp Ala Ala Gly Tyr Ile Thr Val Arg Phe Leu Ile Gly Phe Ser Leu
130 135 140
Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser
145 150 155 160
Lys Ile Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met
165 170 175
Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile
180 185 190
Gln Lys Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe
195 200 205
Val Pro Gly Met Met Hi s Val Val Met Gly Leu Leu Val Leu Thr Met
210 215 220
Gly Gln Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly
225 230 235 240
Glu Met Ala Lys Asp Lys Phe Ser Lys Val Val Trp Gly Ala Val Thr
245 250 255
Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly
260 265 270
Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Phe Asp His
275 280 285
Phe His Leu Asp Leu Arg Thr Ser Gly Thr Ile Ala Ala Cys Phe Gly
290 295 300
Met Ala Asn Ile Val Ala Arg Pro Ala Gly Gly Tyr Leu Ser Asp Leu
305 310 315 320
Gly Ala Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile
325 330 335
Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu Trp Leu Gly Arg Ala Lys
340 345 350
Ala Leu Pro Glu Ser Ile Thr Ala Met Val Leu Phe Ser Ile Cys Ala
355 360 365
Gln Ala Ala Cys Gly Ala Val Phe Gly Val Ile Pro Phe Val Ser Arg
370 375 380
Arg Ser Leu Gly Ile Ile Ser Gly Leu Ser Gly Ala Gly Gly Asn Phe
385 390 395 400
Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Lys Tyr Gly
405 410 415
Thr Gly Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr
420 425 430
Leu Pro Val Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Leu Leu
435 440 445
Pro Pro Asn Ala Asn Ala Thr Glu Glu Glu Phe Tyr Ala Ala Glu Trp
450 455 460
Ser Glu Glu Glu Lys Lys Lys Gly Leu His Ile Pro Gly Gln Lys Phe
465 470 475 480
Ala Glu Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala
485 490 495
Thr Ala Ala Thr Pro Pro Asn Asn Thr Pro Gln His Ala
500 505
<210>77
<211>1796
<212>DNA
＜213〉wheat
<400>77
aagctagcac caagcctcca aggagcaaga agagaagaag ccttgctcga tcaagcaagg 60
tcgaaatgga ggtggaggcc agcgcccatg gcgacacggc ggcgagcaag ttcacgctgc 120
ccgtggactc cgagcacaag gccaagtcct tcagactctt ctccttcgcc aacccccaca 180
tgcgtacctt ccacctctcc tggatatcct tcttcacctg cttcgtctcc accttcgcgg 240
cggcaccgtt ggtgcccatc atccgtgaca acctcaacct cgctaaggcc gacataggga 300
atgccggtgt ggcatctgtg tctgggtcca tcttctccag gcttgccatg ggtgccatct 360
gcgacctttt agggccgcgg tatggctgcg ccttcctcgt catgctctca gcacccactg 420
tgttttgcat ggctgctatc gacgatgcgt caggctacat cgccgtacgc ttcctcattg 480
gcttctccct cgccaccttc gtgtcatgcc aatattggat gagcaccatg ttcaacagta 540
agatcattgg cacggtgaat ggcctcgcgg ccggctgggg caacatgggc ggtggtgcca 600
cacaactcat catgccgctt gttttccatg ccatccaaaa gtgtggtgcc acacccttcg 660
tggcatggcg tattgcctat ttcgtgccgg gaatgatgca catcgtcatg gggttgcttg 720
tgctcactat gggccaagat ctccccgacg gcaaccttgc gagtctccag aagaaggggg 780
acatggccaa ggacaaattc tcgaaggtcc tttggggtgc ggtcaccaac taccggacat 840
ggatattcgt cctcctctac ggctactgca tgggtgtcga gctcaccacc gacaacgtca 900
tcgccgagta ctactacgac cacttccacc ttgaccttcg cgccgctggc accattgccg 960
cttgcttcgg catggccaac atcgtcgcgc gtcctatggg tggctatctc tctgaccttg 1020
gtgcccgcta cttcggcatg cgtgctcggc tctggaacat ctggatcctc cagaccgctg 1080
gtggcgcttt ctgcatctgg ctcggtcgtg catcggccct tcctgcctca gtcacggcca 1140
tggtcctctt ttccatttgt gcacaagctg cttgtggtgc tgtatttggc gtcgcaccct 1200
tcgtttccag gcgttccctt ggcatcatct ccgggctgac cggcgctggt ggcaatgttg 1260
gcgcagggct aacgcaactt cttttcttca catcgtcgca atactccacc gggaggggtc 1320
tcgagtacat gggcatcatg atcatggcat gcacattacc cgtcgctctg gtgcacttcc 1380
cccaatgggg ctccatgttc ttcccggcta gcgctgatgc cacggaagag gaatactatg 1440
cttctgagtg gtcggaggag gagaagggca agggtctcca tattacaggc caaaagttcg 1500
cagagaactc ccgctcagag cgcggcaggc gcaacgtcat ccttgccaca tccgccacgc 1560
cacccaacaa cacaccccag cacgtataag gcccttattt ttatgtcacc taagaatttt 1620
actgttcatc acgtatatat acaaaccgta tatctacgtc tgcagcccca gcgtaataag 1680
ttgtatgggg atttatgttt ctactagtaa acttaaggaa acgctgcttt tgcgttcctg 1740
ctctgtacgc atgaaatgta atatcaattt gagtccgaaa ttactacaaa aaaaaa 1796
<210>78
<21l>507
<212>PRT
＜213〉wheat
<400>78
Met Glu Val Glu Ala Ser Ala His Gly Asp Thr Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Ala Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Thr
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Tyr Asp His Phe His
275 280 285
Leu Asp Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Met Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln
325 330 335
Thr Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu
340 345 350
Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala
355 360 365
Ala Cys Gly Ala Val Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser
370 375 380
Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala
385 390 395 400
Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly
405 410 415
Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro
420 425 430
Val Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Phe Pro Ala
435 440 445
Ser Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu
450 455 460
Glu Glu Lys Gly Lys Gly Leu His Ile Thr Gly Gln Lys Phe Ala Glu
465 470 475 480
Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala Thr Ser
485 490 495
Ala Thr Pro Pro Asn Asn Thr Pro Gln His Val
500 505
<210>79
<211>1744
<212>DNA
＜213〉wheat
<400>79
gcagtataat caagcaagct agctgcaagc cgaggagcct agctcgatca agcaaggtcg 60
aaatggaggt ggagtctagc gcccatggcg acgccgcggc gagcaagttc acgctgcctg 120
tggactccga gcacaaggcc aagtccttca ggctcttctc cttcgccaac ccccacatgc 180
gcaccttcca cctctcctgg atatccttct tcacctgctt tgtctccacc ttcgccgccg 240
cgccgttggt gcccatcatc cgtgacaacc tcaacctcgc caaggccgac atagggaatg 300
ccggtgtggc atctgtgtca gggtccatct tctccaggct tgccatgggc gccgtctgcg 360
accttttggg gccgcggtat ggctgtgcct tcctcgtcat gctctcagcg ccaacggtct 420
tctgcatggc cgtcatcgat gacgcctcgg ggtacatcgc tgtacgcttc ctcattggct 480
tctccctcgc cgcctttgtg tcctgccaat actggatgag caccatgttc aacagtaaga 540
tcattggcac ggtgaatggc ctcgcggccg gctggggcaa catggggggc ggtgccacac 600
aactcattat gccacttgtt ttccatgcca tccaaaagtg cggtgccaca cccttcgtgg 660
catggcgtat cgcctacttc gtgccgggaa tgatgcacat cgtcatgggg ttgcttgtcc 720
tcacaatggg ccaagatctc cccgacggca accttgcgag cctccagaag aagggagaca 780
tggccaagga caagttctcc aaggtccttt ggggcgccgt caccaactac cggacatgga 840
tcttcgtcct cctctacggc tactgcatgg gtgtcgagct caccactgac aacgtcatcg 900
ccgagtacta ctacgaccat ttccacctac accttcgcgc tgcaggcacc atcgccgcct 960
gctttggcat ggccaatatc gtcgcacgtc ctatgggagg ttacctctct gaccttggcg 1020
ctcgctactt tggtatgcgt gctcgcctat ggaacatctg gatcctccag accgccggcg 1080
gcgctttctg catctggctc ggtcgtgcat cggccctccc cgcctcagtg actgccatgg 1140
ttctcttctc catctgcgcc caagctgcat gtggcgctgt ctttggtgtt gcaccattcg 1200
tttccaggcg ttcccttggc atcatctctg ggttaaccgg cgctggtggc aatgtgggcg 1260
cggggctcac acaacttctc ttcttcactt cgtcgcaata ctccaccggg aggggtctcg 1320
agtacatggg catcatgatc atggcatgca cattacctat cactctggtg cacttcccac 1380
aatggggctc catgttcttc ccggccagtg ctgatgctac ggaggaggag tactacgctt 1440
ccgagtggtc agaggaggag aagggcaagg gtctccatat cgcaggccag aagtttgcag 1500
agaactcccg ctcggagcgt ggtaggcgca atgttatcct cgccacatcc gccacgccgc 1560
ccaacaatac accccagcat gtataaggcc cttgttttct gtcacctatg aattgtacgg 1620
ttcgtcacgt acatatacaa accgtatatc tacgtcggca gccccagcgt aataagttgt 1680
atggggattt atctttctac tagtaaactt aaggaaacgc tggttttgcg ttcctgctct 1740
gtac 1744
<210>80
<211>507
<212>PRT
＜213〉wheat
<400>80
Met Glu Val Glu Ser Ser Ala His Gly Asp Ala Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Val Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Ala
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Tyr Asp His Phe His
275 280 285
Leu His Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Met Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln
325 330 335
Thr Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu
340 345 350
Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala
355 360 365
Ala Cys Gly Ala Val Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser
370 375 380
Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala
385 390 395 400
Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly
405 410 415
Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro
420 425 430
Ile Thr Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Phe Pro Ala
435 440 445
Ser Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu
450 455 460
Glu Glu Lys Gly Lys Gly Leu His Ile Ala Gly Gln Lys Phe Ala Glu
465 470 475 480
Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala Thr Ser
485 490 495
Ala Thr Pro Pro Asn Asn Thr Pro Gln His Val
500 505
<210>81
<211>1704
<212>DNA
＜213〉barley
<400>81
caccactgca agcatattta ggcttagtta gctccaagga gcaaagctaa aaagaaccta 60
gctaggctag ctcgatccag ctagctcagt agatatggag gtggaggccg gagctcatgg 120
cgatgcggcg gcgagcaagt tcacgctgcc cgtggactcc gagcacaagg ccaagtcctt 180
caggctcttc tccttcgcca acccgcacat gcgcaccttc cacctctcgt ggatctcctt 240
cttcacctgc ttcgtctcca cctttgccgc tgctccgttg gtgcccatca tccgcgacaa 300
cctcaacctc gccaaggccg acatcggcaa tgccggtgtg gcgtccgtgt ccggctccat 360
cttctcgagg ctcgccatgg gcgccatttg tgacctgctt ggcccgcggt acggttgtgc 420
ctttctcgtc atgctatcgg cgccaaccgt cttctgcatg gccgtcatcg acgacgcgtc 480
gggatacatc gcagtccgct tcctcatcgg cttctccctc gcaaccttcg tgtcatgcca 540
gtactggatg agcacaatgt tcaacagtaa aatcatcggc acggttaatg gcctcgcagc 600
cgggtggggc aacatgggtg gcggggccac acagctcatc atgcccctcg tcttccatgc 660
catccaaaag tgtggtgcca caccctttgt ggcatggcgt atcgcctact tcgtgccggg 720
gatgatgcac atcgtgatgg gcctactcgt gctcaccatg ggacaagacc tccctgatgg 780
gaacctcgca agcctgcaga agaagggaga catggccaag gacaagttct ccaaggtcct 840
ttggggcgcc gttaccaact accggacatg gatctttgtc ctcctctatg gctactgcat 900
gggtgtcgag ctcaccactg gcaatgtcat tgccgagtac tacttcgatc acttccacct 960
aaacctccgt gccgccggta ccatcgccgc ttgcttcggc atggccaaca tcgtcgcacg 1020
tcctatgggc ggctacctct ccgaccttgg tgctcgctac ttcggtatgc gtgctcgcct 1080
ttggaacatc tggatccttc agacagctgg cggcgccttt tgcatctggc ttgggcgcgc 1140
ctcggccctc cccgcctcag tgactgccat ggtcctcttc tccatctgcg cccaggctgc 1200
gtgtggtgct atctttggtg tcgaaccctt cgtctccagg cgttcccttg gcatcatttc 1260
cgggttgacc ggtgctggtg gaaacgtggg cgcagggctc acacagcttc tcttcttcac 1320
ttcgtcgcaa tactccactg gcaggggtct tgagtacatg ggcatcatga tcatggcatg 1380
caccttaccc gtcgctctcg ttcacttccc tcagtggggc tctatgttct tggctgccag 1440
tgccgacgcc acggaggagg agtactacgc ctcagagtgg tcagaggagg agaagagcaa 1500
gggtctccat atcgcaggac aaaagtttgc tgagaactcc cgctcggaac gcggcaggcg 1560
caacgtcatc cttgccacat ccgccacacc acccaacaac acgcccctac acgtataagt 1620
ttcaaatttt gtgttacaca agaaatgtac atcttgctga gtatatatac acatcgtata 1680
ttttagtaaa aaaaaaaaaa aaaa 1704
<210>82
<211>507
<212>PRT
＜213〉barley
<400>82
Met Glu Val Glu Ala Gly Ala His Gly Asp Ala Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Val Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Thr
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Lys Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Gly Asn Val Ile Ala Glu Tyr Tyr Phe Asp His Phe His
275 280 285
Leu Asn Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Met Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Arg Leu Trp Asn Ile Trp Ile Leu Gln
325 330 335
Thr Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu
340 345 350
Pro Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala
355 360 365
Ala Cys Gly Ala Ile Phe Gly Val Glu Pro Phe Val Ser Arg Arg Ser
370 375 380
Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala
385 390 395 400
Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly
405 410 415
Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro
420 425 430
Val Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Leu Ala Ala
435 440 445
Ser Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu
450 455 460
Glu Glu Lys Ser Lys Gly Leu His Ile Ala Gly Gln Lys Phe Ala Glu
465 470 475 480
Asn Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Leu Ala Thr Ser
485 490 495
Ala Thr Pro Pro Asn Asn Thr Pro Leu His Val
500 505
<210>83
<211>1825
<212>DNA
＜213〉barley
<400>83
tctcagttgc cactgcagct gatcaagcaa gctagctcca aacctccaag gaggaagcag 60
agaaggagac tagctcgatc aagcaaggtc caaatggagg tggaggctgg tgcccatggc 120
gacacggcgg cgagcaagtt cacgttgccc gtggactccg agcacaaggc caagtccttc 180
aggctcttct ccttcgccaa cccacacatg cgcacctttc acctatcgtg gatatccttc 240
ttcacatgct tcgtctccac ctttgccgcg gcgcccctgg tgcccatcat ccgcgacaac 300
ctgaacctcg ccaaggccga catagggaat gccggtgtgg catctgtgtc tgggtctatc 360
ttctcgaggc ttgccatggg cgccatctgc gaccttttgg ggccgcggta tgggtgtgcc 420
ttcctcgtca tgctctcagc gccaaccgtc ttctgcatgg ccgtcatcga tgacgcctca 480
gggtacatcg ccgtacgctt cctcatcggc ttctcccttg ccacctttgt gtcgtgccaa 540
tactggatga gcaccatgtt caacagtaaa atcatcggca cggtcaatgg cctcgcggcc 600
ggctggggca acatgggcgg tggtgccaca caactcatca tgccgcttgt tttccacgcc 660
atccaaaaat gtggtgccac accatttgtg gcatggcgta ttgcctactt cgtgcccgga 720
atgatgcaca tcgtgatggg cttgctggta ctcaccatgg ggcaagatct ccctgatggg 780
aacctcgcga gcctccagaa gagaggagac atggccaagg acaagttctc caaggtcctt 840
tggggcgccg tcaccaacta ccggacatgg atctttgtcc tcctatatgg ctactgcatg 900
ggtgtcgaac tcaccactga caatgtcatt gccgagtact acttcgacca cttccaccta 960
gaccttcgcg ccgctggtac catcgccgcc tgcttcggta tggccaacat agtcgcacgt 1020
cctatgggcg gctacctctc tgaccttggc gcccgctatt tcggcatgcg tgccctttgg 1080
aacatctgga tcctccaaac cgctggtggc gctttctgca tctggctcgg tcgtgcatcg 1140
gccctccctg cctcggtgac cgccatggtc ctcttctcca tctgtgccca ggctgcctgt 1200
ggtgctatct ttggtgtcgc acccttcgtc tccaggcgtt cccttggcat catttccggg 1260
ttgaccggtg ccggtggaaa cgtgggcgca ggactcacac aacttctatt cttcacctca 1320
tcgcaatact ccactggtag gggtctcgag tacatgggca tcatgatcat ggcatgcacg 1380
ctgcccgtcg ctcttgtgca ctttccgcaa tggggatcca tgttcttccc ggccagcgct 1440
gatgccactg aggaggagta ctatgcttcc gagtggtcgg aggaggagaa gggcaagggt 1500
ctccatatcg caggccaaaa gttcgccgag aactcccgct cggagcgcgg caggcgcaac 1560
gtcatctttg ccacatccgc cacgccgccc aacaacacac cccagcaggt ataaggcatt 1620
tttttttgtt acctatgaat tttacagctc atggcgtata tatacaaaca gtatatttac 1680
gtttgcagcc ccagcgtaat aagttgtatg ggggtttatc tttttactat ggtaaaccta 1740
aggacatgta ttgtcaaatt gagtccgaaa ttaatacatg aacagtgttg atgtttgtgt 1800
atgcttgaaa aaaaaaaaaa aaaaa 1825
<210>84
<211>506
<212>PRT
＜213〉barley
<400>84
Met Glu Val Glu Ala Gly Ala His Gly Asp Thr Ala Ala Ser Lys Phe
1 5 10 15
Thr Leu Pro Val Asp Ser Glu His Lys Ala Lys Ser Phe Arg Leu Phe
20 25 30
Ser Phe Ala Asn Pro His Met Arg Thr Phe His Leu Ser Trp Ile Ser
35 40 45
Phe Phe Thr Cys Phe Val Ser Thr Phe Ala Ala Ala Pro Leu Val Pro
50 55 60
Ile Ile Arg Asp Asn Leu Asn Leu Ala Lys Ala Asp Ile Gly Asn Ala
65 70 75 80
Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser Arg Leu Ala Met Gly
85 90 95
Ala Ile Cys Asp Leu Leu Gly Pro Arg Tyr Gly Cys Ala Phe Leu Val
100 105 110
Met Leu Ser Ala Pro Thr Val Phe Cys Met Ala Val Ile Asp Asp Ala
115 120 125
Ser Gly Tyr Ile Ala Val Arg Phe Leu Ile Gly Phe Ser Leu Ala Thr
130 135 140
Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met Phe Asn Ser Lys Ile
145 150 155 160
Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn Met Gly Gly
165 170 175
Gly Ala Thr Gln Leu Ile Met Pro Leu Val Phe His Ala Ile Gln Lys
180 185 190
Cys Gly Ala Thr Pro Phe Val Ala Trp Arg Ile Ala Tyr Phe Val Pro
195 200 205
Gly Met Met His Ile Val Met Gly Leu Leu Val Leu Thr Met Gly Gln
210 215 220
Asp Leu Pro Asp Gly Asn Leu Ala Ser Leu Gln Lys Arg Gly Asp Met
225 230 235 240
Ala Lys Asp Lys Phe Ser Lys Val Leu Trp Gly Ala Val Thr Asn Tyr
245 250 255
Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met Gly Val Glu
260 265 270
Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Tyr Phe Asp His Phe His
275 280 285
Leu Asp Leu Arg Ala Ala Gly Thr Ile Ala Ala Cys Phe Gly Met Ala
290 295 300
Asn Ile Val Ala Arg Pro Met Gly Gly Tyr Leu Ser Asp Leu Gly Ala
305 310 315 320
Arg Tyr Phe Gly Met Arg Ala Leu Trp Asn Ile Trp Ile Leu Gln Thr
325 330 335
Ala Gly Gly Ala Phe Cys Ile Trp Leu Gly Arg Ala Ser Ala Leu Pro
340 345 350
Ala Ser Val Thr Ala Met Val Leu Phe Ser Ile Cys Ala Gln Ala Ala
355 360 365
Cys Gly Ala Ile Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser Leu
370 375 380
Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Val Gly Ala Gly
385 390 395 400
Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Gln Tyr Ser Thr Gly Arg
405 410 415
Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys Thr Leu Pro Val
420 425 430
Ala Leu Val His Phe Pro Gln Trp Gly Ser Met Phe Phe Pro Ala Ser
435 440 445
Ala Asp Ala Thr Glu Glu Glu Tyr Tyr Ala Ser Glu Trp Ser Glu Glu
450 455 460
Glu Lys Gly Lys Gly Leu His Ile Ala Gly Gln Lys Phe Ala Glu Asn
465 470 475 480
Ser Arg Ser Glu Arg Gly Arg Arg Asn Val Ile Phe Ala Thr Ser Ala
485 490 495
Thr Pro Pro Asn Asn Thr Pro Gln Gln Val
500 505
<210>85
<211>1885
<212>DNA
＜213〉peach (Prunus persica)
<400>85
catttagtct aagtagtttc taaattcgaa acttgagtgc tgaaactcga gattcaaaat 60
ccaaactcca aaccccaaat tcaaaacccc aaaaacatgg ccgaagtcga aggtgaaccc 120
ggaagctcca tgcatggagt gacaggcaga gagcaaacct ttgcgttctc ggtagcttcc 180
cccatcgtcc caacagaccc aacagccaaa tttgacctac cagttgattc agagcacaag 240
gccaaagttt tcaaaatctt ctctttggcc aaccctcaca tgagaacttt ccacttgtct 300
tggatctctt tcttcacttg ctttgtctca acttttgcag cggccccact tgtccctata 360
atccgagaca acctcaacct cacaaagcaa gacattggaa atgctggggt tgcctctgtc 420
tcaggcagca tattctcaag acttgtaatg ggtgcagtgt gtgatttgct agggccacga 480
tatgggtgtg cctttctcat aatgctcagc gcacccactg tgttttgcat gtcatttgta 540
tctgatgctg ggggctactt ggcagtgaga ttcatgattg gtttttcgct tgctacattc 600
gtgtcatgcc agtattggat gagtaccatg tttaacagta agattattgg gctggttaat 660
gggacagctg ctgggtgggg aaacatgggt ggtggggcca cccagctctt gatgccattg 720
gtgtttgata taattggaag agttggtgca actcctttca ctgcttggag aattgccttt 780
ttcattcctg gctggcttca tgtcattatg ggaataatgg tcttgaccct tggccaagac 840
ttgcctgatg ggaatcttgc tgccctgcaa aagaagggtg atgttgccaa agatcaattc 900
tccaaggtat tgtggcatgc tgtaacaaat tacaggacat ggatctttgt ccttctctat 960
ggctactcca tgggtgttga attgtccact gataatgtca ttgctgaata cttctatgac 1020
aggttcaatc tcaagcttca cacagctgga atcattgctg caacatttgg catggccaac 1080
ctagtagccc gtccctttgg aggatttgcg tctgatcgag cagccaggta ctttggcatg 1140
aggggcaggc tatggactct ttggatcctc caaacactag gaggagtctt ctgcatctgg 1200
ctcggccgag caaactcact ccccattgcg gtctttgcca tgatcctctt ctctgtagga 1260
gcccaagctg catgcggagc cacctttggc gtcatcccct tcatctcccg gcgatccctc 1320
ggcatcatat cgggcctcac tggagcgggt gggaacttcg ggtccgggct gacccaacta 1380
gtgttcttct caagctcagc attctcaact gcgacagggt tgtctctgat gggggtaatg 1440
atcgtgtgct gcacacttcc agtgactttg gtgcacttcc ctcagtgggg gagcatgttc 1500
cttccgcctt caaaagatgt cgtgaaatcg acggaagagt tttactatgg agctgagtgg 1560
aatgaggagg agaagcagaa ggggctacac cagcagagtt tgaggtttgc agagaatagt 1620
aggtctgagc gtggtaggcg tgttgcctca gctccaaccc cacccaacac cacaccttcc 1680
catgtttagg ttatgttatg atctcatgag aattgtttct ttgaaatgct ttgcaaactc 1740
ctcatgcgcc caattattct ccttaagttg accgagaagc ttacttctct cttggggaaa 1800
ttttttcttt attattatca gttttttccc aagcatataa gtgaactgat gattattttt 1860
atttcagaaa aaaaaaaaaa aaaaa 1885
<210>86
<211>530
<212>PRT
＜213〉peach
<400>86
Met Ala Glu Val Glu Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Thr Phe Ala Phe Ser Val Ala Ser Pro Ile Val Pro
20 25 30
Thr Asp Pro Thr Ala Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Ile Phe Ser Leu Ala Asn Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Gln Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Asp Ala Gly Gly Tyr Leu Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Leu Met Pro Leu
195 200 205
Val Phe Asp Ile Ile Gly Arg Val Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Ile Met Gly Ile
225 230 235 240
Met Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Ala Ala
245 250 255
Leu Gln Lys Lys Gly Asp Val Ala Lys Asp Gln Phe Ser Lys Val Leu
260 265 270
Trp His Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Tyr Asp Arg Phe Asn Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Val Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ala Ser Asp Arg Ala Ala Arg Tyr Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Ile Trp
355 360 365
Leu Gly Arg Ala Asn Ser Leu Pro Ile Ala Val Phe Ala Met Ile Leu
370 375 380
Phe Ser Val Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Val Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Ser Ser Ala Phe Ser Thr Ala Thr Gly Leu Ser Leu Met Gly Val Met
435 440 445
Ile Val Cys Cys Thr Leu Pro Val Thr Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Val Val Lys Ser Thr Glu
465 470 475 480
Glu Phe Tyr Tyr Gly Ala Glu Trp Asn Glu Glu Glu Lys Gln Lys Gly
485 490 495
Leu His Gln Gln Ser Leu Arg Phe Ala Glu Asn Ser Arg Ser Glu Arg
500 505 510
Gly Arg Arg Val Ala Ser Ala Pro Thr Pro Pro Asn Thr Thr Pro Ser
515 520 525
His Val
530
<210>87
<211>1962
<212>DNA
＜213〉peach
<400>87
gcttcattta gtctaagtag tttctaaatt cgaaactcga gttttgaaac tcgagattca 60
aaatccaaac tccaaacccc aaataatggc cgaagtcgaa ggtgaacccg gaagctccat 120
gcatggagtg acaggcagag agcaaacctt tgcgttctca gtagcttccc ccatcgtccc 180
aacagaccca acagctaaat ttgacctacc tgttgattca gagcacaagg ccaaagtttt 240
caaaatcttc tctttggcca acccgcacat gagaaccttc cacttgtctt ggatctcctt 300
cttcacttgc tttgtctcaa cttttgcagc ggccccactt gtccctataa tccgagacaa 360
cctcaacctc acaaagcaag acattggaaa tgctggggtt gcctctgtct caggcagcat 420
attctcaaga cttgtaatgg gtgcagtgtg tgatttgcta gggccacgat atgggtgtgc 480
ctttctcata atgctcagcg cacccactgt gttttgcatg tcatttgtat ctgatgctgg 540
gggctacttg gcagtgagat tcatgattgg tttttcgctt gctacattcg tgtcatgcca 600
gtattggatg agtaccatgt ttaacagtaa gattattggg ctggttaatg ggacagctgc 660
tgggtgggga aacatgggtg gtggggccac ccagctcttg atgccattgg tgtttgatat 720
aattggaaga gttggtgcaa ctcctttcac tgcttggaga attgcctttt tcatccctgg 780
ctggcttcat gtcattatgg gaataatggt cttgaccctt ggccaagact tgcctgatgg 840
aaatcttgct gccctgcaaa agaagggtga tgttgccaaa gatcaattct ccaaggtatt 900
gtggcatgct ataacaaact acaggacatg gatctttgtc cttctctatg gctactccat 960
gggtgttgaa ttgtccattg ataatgtcat tgctgaatac ttctatgaca ggttcaatct 1020
caagcttcac acagctggaa tcattgctgc agcatttggc atggccaaca tagtggcccg 1080
tccctttgga ggatttgcgt ctgatcgagc agccaggtac tttggcatga ggggcaggct 1140
atggactctt tggatcctcc aaacactagg aggagtcttt tgcatctggc tcggccgagc 1200
aaactcactc cccattgcgg tctttgccat gatcctcttc tctgtaggag cccaagctgc 1260
atgcggagcc acctttggcg tcatcccctt catctcccgg cgatccctcg gcatcatatc 1320
gggcctcact ggagcgggtg ggaacttcgg atccgggctg acccaactag tgttcttctc 1380
aagcgcagca ttctcaactg cgacagggtt gtctctgatg ggggtaatga tcgtgtgctg 1440
cacacttcca gtgactttgg tgcacttccc tcagtggggg agcatgttcc ttccgccttc 1500
aaaagatgtc gtgaaatcaa cggaagagtt ttactatgga gctgagtgga atgaggagga 1560
gaagcagaag gggctacacc agcagagttt gaggtttgca gagaatagta ggtctgagcg 1620
tggtaggcgt gttgcctcag ctccaacccc acccaacacc acaccttccc atgtttagtc 1680
aaacaaggga aaaaggtcaa catgcaccaa ggtgcatatg tggtgtggaa caaaccaata 1740
atcagagaag tgctattttg tatcatctca atcaatcata tgtgtactgt atttctatta 1800
gaaatacaag agatgatcag ttgagttgat acaagtagtt ttatttgtaa tgaaaataga 1860
gttgccatat agcgtgtgcc tctatatatg tatcttgtac aeaaatgttt ttactttcaa 1920
ggtttaaact tatgataatt ttcaaaaaaa aaaaaaaaaa aa 1962
<210>88
<211>530
<212>PRT
＜213〉peach
<400>88
Met Ala Glu Val Glu Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Thr Phe Ala Phe Ser Val Ala Ser Pro Ile Val Pro
20 25 30
Thr Asp Pro Thr Ala Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Ile Phe Ser Leu Ala Asn Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Gln Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Asp Ala Gly Gly Tyr Leu Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Leu Met Pro Leu
195 200 205
Val Phe Asp Ile Ile Gly Arg Val Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Ile Met Gly Ile
225 230 235 240
Met Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Ala Ala
245 250 255
Leu Gln Lys Lys Gly Asp Val Ala Lys Asp Gln Phe Ser Lys Val Leu
260 265 270
Trp His Ala Ile Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Ile Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Tyr Asp Arg Phe Asn Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Ala Phe Gly Met Ala Asn Ile Val Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ala Ser Asp Arg Ala Ala Arg Tyr Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Ile Trp
355 360 365
Leu Gly Arg Ala Asn Ser Leu Pro Ile Ala Val Phe Ala Met Ile Leu
370 375 380
Phe Ser Val Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Val Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Ser Ala Ala Phe Ser Thr Ala Thr Gly Leu Ser Leu Met Gly Val Met
435 440 445
Ile Val Cys Cys Thr Leu Pro Val Thr Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Val Val Lys Ser Thr Glu
465 470 475 480
Glu Phe Tyr Tyr Gly Ala Glu Trp Asn Glu Glu Glu Lys Gln Lys Gly
485 490 495
Leu His Gln Gln Ser Leu Arg Phe Ala Glu Asn Ser Arg Ser Glu Arg
500 505 510
Gly Arg Arg Val Ala Ser Ala Pro Thr Pro Pro Asn Thr Thr Pro Ser
515 520 525
His Val
530
<210>89
<211>1890
<212>DNA
＜213〉soybean
<400>89
tcacactttc ttccttaatt ttctagctct tgctacgtac ttgaattcaa ttagttatta 60
atggctgaga ttgagggttc tcccggaagc tccatgcatg gagtaacagg aagagaacaa 120
acatttgtag cctcagttgc ttctccaatt gtccctacag acaccacagc caaatttgct 180
ctcccagtgg attcagaaca caaggccaag gttttcaaac tcttctccct ggccaatccc 240
cacatgagaa ccttccacct ttcttggatc tccttcttca cctgcttcgt ctcgacattc 300
gcagcagcac ctcttgtgcc catcatccgc gacaacctta acctcaccaa aagcgacatt 360
ggaaacgccg gggttgcttc tgtctccgga agcatcttct caaggctcgc aatgggtgca 420
gtctgtgaca tgttgggtcc acgctatggc tgcgccttcc tcatcatgct ttcggcccct 480
acggtgttct gcatgtcctt tgtgaaagat gctgcggggt acatagcggt tcggttcttg 540
attgggttct cgttggcgac gtttgtgtcg tgccagtact ggatgagcac gatgttcaac 600
agtaagatta tagggcttgc gaatgggact gctgcggggt gggggaacat gggtggtgga 660
gccactcagc tcataatgcc tttggtgtat gagcttatca gaagagctgg ggctactccc 720
ttcactgctt ggaggattgc cttctttgtt ccgggtttca tgcatgtcat catggggatt 780
cttgtcctca ctctaggcca ggacttgcct gatggaaacc tcggggcctt gcggaagaag 840
ggtgatgtag ctaaagacaa gttttccaag gtgctatggt atgccataac aaattacagg 900
acatggattt ttgctctcct ctatgggtac tccatgggag ttgaattaac aactgacaat 960
gtcattgctg agtatttcta tgacagattt aatctcaagc tacacactgc tggaatcatt 1020
gctgcttcat ttggaatggc aaacttagtt gctcgacctt ttggtggata tgcttcagat 1080
gttgcagcca ggctgtttgg catgagggga agactctgga ccctttggat cctccaaacc 1140
ttaggagggg ttttctgtat ttggcttggc cgtgccaatt ctcttcctat tgctgtattg 1200
gccatgatcc tgttctctat aggagctcaa gctgcatgtg gtgcaacttt tggcatcatt 1260
cctttcatct caagaaggtc tttggggatc atatcaggtc taactggtgc aggtggaaac 1320
tttgggtctg gcctcaccca attggtcttc ttttcaacct ccaaattctc tactgccaca 1380
ggtctctcct tgatgggtgt aatgatagtg gcttgcactc taccagtgag tgttgttcac 1440
ttcccacagt ggggtagcat gtttctacca ccctcaaaag atgtcagcaa atccactgaa 1500
gaattctatt acacctctga atggaatgag gaagagaagc agaagggttt gcaccagcaa 1560
agtctcaaat ttgctgagaa tagccgatct gagagaggaa agcgagtggc ttcagcacca 1620
acacctccaa atgcaactcc cactcatgtc tagccatagc acttcaatca aagaagatca 1680
tgaaacataa ttactgagca gtattgggaa tgaagaacca tgagttgaag aattttctaa 1740
taagaaatct tgtaacatgt agacatagaa tgttctggtt ctggtttgcg tgtggtgtaa 1800
gagttgtcta cttgtggtaa gtcataagta tcataatcag tatgtcaatg cagatcttga 1860
tgctgagtat caatagtatc aaaaaaaaaa 1890
<210>90
<211>530
<212>PRT
＜213〉soybean
<400>90
Met Ala Glu Ile Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Thr Phe Val Ala Ser Val Ala Ser Pro Ile Val Pro
20 25 30
Thr Asp Thr Thr Ala Lys Phe Ala Leu Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Leu Phe Ser Leu Ala Asn Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Ser Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Ala Met Gly Ala Val Cys Asp Met Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Lys Asp Ala Ala Gly Tyr Ile Ala Val Arg Phe Leu
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Ala Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu
195 200 205
Val Tyr Glu Leu Ile Arg Arg Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Val Pro Gly Phe Met His Val Ile Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Gly Ala
245 250 255
Leu Arg Lys Lys Gly Asp Val Ala Lys Asp Lys Phe Ser Lys Val Leu
260 265 270
Trp Tyr Ala Ile Thr Asn Tyr Arg Thr Trp Ile Phe Ala Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Tyr Asp Arg Phe Asn Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Ser Phe Gly Met Ala Asn Leu Val Ala Arg Pro Phe Gly Gly
325 330 335
Tyr Ala Ser Asp Val Ala Ala Arg Leu Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Ile Trp
355 360 365
Leu Gly Arg Ala Asn Ser Leu Pro Ile Ala Val Leu Ala Met Ile Leu
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Thr Ser Lys Phe Ser Thr Ala Thr Gly Leu Ser Leu Met Gly Val Met
435 440 445
Ile Val Ala Cys Thr Leu Pro Val Ser Val Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Val Ser Lys Ser Thr Glu
465 470 475 480
Glu Phe Tyr Tyr Thr Ser Glu Trp Asn Glu Glu Glu Lys Gln Lys Gly
485 490 495
Leu His Gln Gln Ser Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Ala Ser Ala Pro Thr Pro Pro Asn Ala Thr Pro Thr
515 520 525
His Val
530
<210>91
<211>1990
<212>DNA
<213>Lotus japonicus
<400>91
gcacaagttt ttctctgttg catgttctgt ttctgaatta ctttaattac ttcacacaac 60
aacattcaac aatggttgaa attgaaggat ctccaggaac ctccatgcat ggagtaacag 120
ggagggaaca aaccttcata tcctcagttt cttccccaat ggtcccaaca gacaccacag 180
ccaaattcga tttaccagtt gattcacagc acaaagccaa agttttcaaa ctgttctcat 240
tagctaatcc tcacatgaca accttccact tatcatggat ttccttcttc acctgctttg 300
tctctacctt tgcagcagct cctcttgtcc ccatcatccg agacaacctc aatctcacca 360
aaagcgacat tggcaacgcc ggtgtcgcgt ctgtctccgg cagcattttc tccaggctca 420
ccatgggtgt aatttgtgac ctcttaggcc cacgttacgg gtgtgccttc ctgatcatgc 480
tgtctgcacc aactgtgttc tgcatgtcgt ttgtgaatga tgctgcaggc tacattgtgg 540
ttcggttcat gatcgggttc tcattggcca cgtttgtttc gtgccagtac tggatgagta 600
ccatgtttaa cagtaagatt attgggcttg ttaatggaac tgcagctggt tggggtaaca 660
tgggtggtgg agccactcag ctcatcatgc ctattgtcta tgaattgatc aggagagcgg 720
ggtccactgg gttcactgct tggaggattg ccttcttcat ccctggtttc atgcatgttt 780
tcatggggat ccttgtctta acccttggcc aagacttgcc tgatggcaac ctcagtgccc 840
tccagaagaa gggtgatgtt gctaaagaca aattctccaa ggtgttatgg tatgccataa 900
caaattacag gacatggatc tttgcacttc tctatggata ctctatggga gtcgaattaa 960
ccactgacaa tgtcattgct gagtattttt atgacagatt taatctgaag ttgcacacag 1020
ttggaatcat tgctgcttct tttggaatgg caaatcttgt tgctcgcccc tttggcggat 1080
atgtatctga tgttgcagcc cggttgtttg gtatgagagg aagactgtgg actctgtgga 1140
tcctccaaac acttggagga gtgttctgta tatggcttgg gcgtgcgaat tcacttccaa 1200
ttgcggtgtt ggcgatgatc cttttctcag ttggagctca agctgcttgt ggtgcaacat 1260
ttggaatcat tcctttcatc tcaaaaaggt ctttggggat catttcaggt ctaactggtg 1320
caggtggcaa ctttggttct ggattgacac agttggtatt cttttctact tcaagattct 1380
ccacaggtgc aggattgtca tggatgggtg tgatgattgt tggttgcact cttccggtga 1440
ctcttgttca cttcccacag tggggtagca tgttccttcc tccttcaaaa gacatcaaca 1500
agtccagtga ggaacactat tacactgctg aatgggatga ggaagagagg aagaaggggt 1560
tgcactctca aagtcttaag tttgcagaga atagtcgctc tgagagagga aaacgtgttt 1620
cctctgctcc aactcctcca aacacaaccc ctacccatgt ctaagatgga tggatgatca 1680
ccacagtgga aagctaatga ggaaaagagt atatggatcc cttgattgaa taatttatcc 1740
ttaagccttt aaagatactt tggcatccag aaatttggtg tttgcccttc aaattctact 1800
tgttatagaa atttggtgtg attttctata agggactttg gtgtctgtgg gagtgtgggt 1860
ctatcagtta attatacaaa caggtggtgt acaaaaataa tatattgagt tttttaattt 1920
tggtgtgata tatattaatg aatgttactc aaaaaaaaaa aaaaaaaaaa actcgagggg 1980
gggcccggta 1990
<210>92
<211>530
<212>PRT
<213>Lotus japonicus
<400>92
Met Val Glu Ile Glu Gly Ser Pro Gly Thr Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Thr Phe Ile Ser Ser Val Ser Ser Pro Met Val Pro
20 25 30
Thr Asp Thr Thr Ala Lys Phe Asp Leu Pro Val Asp Ser Gln His Lys
35 40 45
Ala Lys Val Phe Lys Leu Phe Ser Leu Ala Asn Pro His Met Thr Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Ser Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Thr Met Gly Val Ile Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Asn Asp Ala Ala Gly Tyr Ile Val Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Ile
195 200 205
Val Tyr Glu Leu Ile Arg Arg Ala Gly Ser Thr Gly Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Phe Met His Val Phe Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Ser Ala
245 250 255
Leu Gln Lys Lys Gly Asp Val Ala Lys Asp Lys Phe Ser Lys Val Leu
260 265 270
Trp Tyr Ala Ile Thr Asn Tyr Arg Thr Trp Ile Phe Ala Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Tyr Asp Arg Phe Asn Leu Lys Leu His Thr Val Gly Ile Ile
305 310 315 320
Ala Ala Ser Phe Gly Met Ala Asn Leu Val Ala Arg Pro Phe Gly Gly
325 330 335
Tyr Val Ser Asp Val Ala Ala Arg Leu Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Ile Trp
355 360 365
Leu Gly Arg Ala Asn Ser Leu Pro Ile Ala Val Leu Ala Met Ile Leu
370 375 380
Phe Ser Val Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Lys Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Thr Ser Arg Phe Ser Thr Gly Ala Gly Leu Ser Trp Met Gly Val Met
435 440 445
Ile Val Gly Cys Thr Leu Pro Val Thr Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Ile Asn Lys Ser Ser Glu
465 470 475 480
Glu His Tyr Tyr Thr Ala Glu Trp Asp Glu Glu Glu Arg Lys Lys Gly
485 490 495
Leu His Ser Gln Ser Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Ser Ser Ala Pro Thr Pro Pro Asn Thr Thr Pro Thr
515 520 525
His Val
530
<210>93
<211>1838
<212>DNA
＜213〉tobacco (Nicotiana tabacum)
<400>93
ttaaagaaga aattaaaaat ggttgatatt gaaggatcac caggtagttc tatgcatgga 60
gttacaggta gagaaccagt tcttgctttc tctgttgcat ctccaatggt acaaactgat 120
accactgcac attttaaagt tccggtcgat tctgaacaca aggccaaggt tttcaaattc 180
tactcatttt caaaacctca cggtctaact tttcaacttt cttggatttc tttctttact 240
tgttttgttt ctacttttgc tgctgcccct ttagttccta ttattagaga caaccttaac 300
ttgaccaaaa tggacgttgg taatgctggg gttgcctctg tttccggaag tattctatct 360
aggcttgcaa tgggtgcgat ttgtgacatg ttgggaccaa gatatggatg cgcgtttctt 420
atcatgttat cagctccgac tgttttttgc atgtctttcg tgtcttcggc tggtggctac 480
gttgctgtga gattcatgat tggattctca ctcgcgacgt ttgtgtcgtg tcaatattgg 540
atgagtacga tgtttaatag tcagattatt ggacttgtta atggaacagc agcaggatgg 600
ggaaatatgg ggggtggagc tactcagctt attatgccta ttctctatga tattattaga 660
agagcaggag ccaccccttt tactgcttgg agaattgctt tctttattcc tggttggctt 720
catgttatta tgggaatttt agtgttaact cttggccaag atttgcctga tggtaacctt 780
gcttccctac agaagaaagg cgatgtttct aaagataagt tctctaagat attgtggtat 840
gctgcaacaa attacaggac atggatcttt gtcctgctct atggttactc catgggagtt 900
gaattgacta cagataatgt gattgctgag tacttctttg atagatttga tctaaagctt 960
cacacggctg gaatcattgc tgccacattt ggtatggcta atctcttagc tcgacctttt 1020
ggaggatggt catcagatat tgcagccaaa cattttggaa tgagaggcag attatggaac 1080
ttatggattt tacaaacact tggtggtgtt ttctgtttct tacttggaaa agcaaacaca 1140
cttcctatgg ctatagcttg gatgatcata ttctccttag gtgctcaagc agcatgtgga 1200
gctacatttg gtattattcc tttcatttcg cgtcgatcgt taggtataat ctcaggtatg 1260
acaggagctg gaggaaattt tggttctgga ttgacacaac tcttgttttt cacaactaca 1320
aaatggtcaa cagaaacagg attgagttat atggggatta tgattatagc ttgtacactt 1380
cctgtatctt tggttcattt cccacaatgg ggaagtatgt ttttgcctcc aactaaagat 1440
cctgttaaga gtactgaaga gcattacttc acgtctgagt acactgaggc tgagaagcaa 1500
aagggtatgc accaaaacag catcaagttt gctgaaaact gtcggtcgga gcgtggtaag 1560
cgtgtgggtt cagctcttac tccgcctaat gtaacgccaa accgtgtctg agcttgagtc 1620
tcacggatca gtacggagaa atagtgattt tgaaaagctt acttgcttaa ttggtgtttg 1680
tatcaacaca ataaacgtgt gatatgtgtc ttttaatgag ctttatatta cccaagtgtg 1740
agtagttaat ttgtattatc actttgtgtt gtgagactga tcttaatatt aaaacagttg 1800
atttggtgta aactttcatt aaaaaaaaaa aaaaaaaa 1838
<210>94
<211>530
<212>PRT
＜213〉tobacco
<400>94
Met Val Asp Ile Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Pro Met Val Gln
20 25 30
Thr Asp Thr Thr Ala His Phe Lys Val Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Ala Met Gly Ala Ile Cys Asp Met Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Ile
195 200 205
Leu Tyr Asp Ile Ile Arg Arg Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Ile Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Ala Ser
245 250 255
Leu Gln Lys Lys Gly Asp Val Ser Lys Asp Lys Phe Ser Lys Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Trp Ser Ser Asp Ile Ala Ala Lys His Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Asn Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Phe Leu
355 360 365
Leu Gly Lys Ala Asn Thr Leu Pro Met Ala Ile Ala Trp Met Ile Ile
370 375 380
Phe Ser Leu Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Thr Thr Lys Trp Ser Thr Glu Thr Gly Leu Ser Tyr Met Gly Ile Met
435 440 445
Ile Ile Ala Cys Thr Leu Pro Val Ser Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Thr Lys Asp Pro Val Lys Ser Thr Glu
465 470 475 480
Glu His Tyr Phe Thr Ser Glu Tyr Thr Glu Ala Glu Lys Gln Lys Gly
485 490 495
Met His Gln Asn Ser Ile Lys Phe Ala Glu Asn Cys Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Gly Ser Ala Leu Thr Pro Pro Asn Val Thr Pro Asn
515 520 525
Arg Val
530
<210>95
<211>1646
<212>DNA
＜213〉tomato
<400>95
gcctaaaatg ggtgatattg aaggatcacc aggaagttca atgcatggtg ttactggtag 60
agagccagtt cttgcatttt cagttgcttc actaattgta ccaactgata catcagccaa 120
tttcaaagtc cctgttgatt ctgaacataa agctaaagtt tttaaatttt attcgttttc 180
gaaacctcat ggactaacgt ttcaactctc atggatttca ttttgtactt gtttcgtatc 240
gacttttgct gcagcccctt tagtccctat tattagggac aatcttaatc taactaaaat 300
ggatgttggt aatgctggag ttgcctctgt ttcgggtagt atcttgtcta ggctagctat 360
gggcgcgatt tgtgacatgt taggtcctag atatggttgc gcgttcctta taatgttatc 420
agccccaact gttttatgta tgtcttttgt gtcatcggct ggagggtacg ttgctgtgag 480
gtttatgatt gggttttcac tagcaacgtt tgtgtcatgt cagtattgga tgagtacgat 540
gtttaatagt caaatcattg gacttgtgaa tggtacagct gctggatggg gtaatatggg 600
tggtggtgct actcaactta ttatgccttt gctctacgat ataatacgta gagcaggggc 660
aaccccgttc actgcttgga gaatcgcatt ttttattcct ggatggcttc atgttattat 720
gggaatttta gttttaactc ttggacaaga tttacctgat ggtaacctcg cttctttaca 780
gaagaaaggc gatgtttcta aagataaatt ctcaaagata ttatggtatg ctgcaacaaa 840
ttacagaaca tggatccttg ttctgctcta tggatactca atgggagttg aattaactac 900
agataacgtg attgctgagt atttcttcga tagatttgat ttgaagcttc atacggctgg 960
aatcatcgct gcaacatttg gcatggctaa cttattagcg cgaccatttg gaggatggtc 1020
atcagatgtt gcagctaaac atttcgggat gagaggcaga ttatggaatt catggatttt 1080
acaaacactt ggtggtgtgt tctgtttact acttggaagg gctactacac ttcctctggc 1140
tattacttgg atgatcatat tctcaatagg tgcacaagca gcatgtggtg taacgtttgg 1200
aattattccc tttatttcgc gaagatcatt aggtataata tcaggtatga caggagctgg 1260
aggcaatttt ggttccggat tgacacaact actgtttttc acgagtacaa agtactcgac 1320
aggaacagga ctaacgtata tggggatgat gatcatcgcg tgtacacttc cagtaatgtt 1380
agttcgtttt ccacagtggg gtagtatgtt tttgcctcca tctaaagatc ctattaaggg 1440
tactgaagaa cattattttg gttctgagta tactgaggat gagaaacaaa agggaatgca 1500
ccagaacagc atcaagttcg cggaaaacag caggacagag cgtgggaaga agcgcgttgg 1560
ttcagcacct actccgccta atgtaacacc aaatcgcgtc tgatggggaa aaaaattaaa 1620
atacttactt cgcagttcat gctcgt 1646
<210>96
<211>531
<212>PRT
＜213〉tomato
<400>96
Met Gly Asp Ile Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Leu Ile Val Pro
20 25 30
Thr Asp Thr Ser Ala Asn Phe Lys Val Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Cys Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Ala Met Gly Ala Ile Cys Asp Met Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Leu Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu
195 200 205
Leu Tyr Asp Ile Ile Arg Arg Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Ile Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Ala Ser
245 250 255
Leu Gln Lys Lys Gly Asp Val Ser Lys Asp Lys Phe Ser Lys Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Leu Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Trp Ser Ser Asp Val Ala Ala Lys His Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Asn Ser Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Leu Leu
355 360 365
Leu Gly Arg Ala Thr Thr Leu Pro Leu Ala Ile Thr Trp Met Ile Ile
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Val Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Ser Thr Lys Tyr Ser Thr Gly Thr Gly Leu Thr Tyr Met Gly Met Met
435 440 445
Ile Ile Ala Cys Thr Leu Pro Val Met Leu Val Arg Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Pro Ile Lys Gly Thr Glu
465 470 475 480
Glu His Tyr Phe Gly Ser Glu Tyr Thr Glu Asp Glu Lys Gln Lys Gly
485 490 495
Met His Gln Asn Ser Ile Lys Phe Ala Glu Asn Ser Arg Thr Glu Arg
500 505 510
Gly Lys Lys Arg Val Gly Ser Ala Pro Thr Pro Pro Asn Val Thr Pro
515 520 525
Asn Arg Val
530
<210>97
<211>1680
<212>DNA
<213>Populus tremula x Populus tremuloides
<400>97
cttccctagt ctgaaactcc tatcacaatc cggtttggaa gagaatagag gaagggaatg 60
gctgacgttg agggttcccc gggtagttcc atgcatggag tgacaggcag agaacaaagc 120
tttgcctttt cggttgcctc tcctacagtc ccgacggata caacagcaaa atttgccttg 180
ccagttgatt ctgagcacaa ggccaaagtg ttcaagattt tctcttttgc taaccctcat 240
atgagaacat ttcacctttc ttggatatca ttcttcactt gttttgtctc cacttttgct 300
gcagcacccc ttgttcctat cattcgggac aacctcaatt taaccaaaag tgacatcggt 360
aatgctggtg ttgcttctgt ctctggaagc atcttctcta ggcttgtaat gggtgcagtt 420
tgtgacctct tggggccgcg atatgggtgt gcgtttctga tcatgctctc agccccaact 480
gtgttttgca tgtcgtttgt ggactcagct ggaggatacc tagcagtccg tttcatgatt 540
ggattctctc ttgcaacgtt cgtgtcatgc cagtactgga tgagcacaat gtttaacagc 600
aagattatcg gactcgtcaa tggaaccgca gccggttggg gcaatatggg tggaggtgca 660
actcagctcg taatgccctt ggtctacgag ctcattaagc gagctggttc aacttcattc 720
agtgcttgga ggatagcatt ttttgtccca ggatggcttc atgttatcat gggaatcttg 780
gtcttgaatc taggccaaga cttgcctgat gggaatctcg gtgccctaaa gaagaagggt 840
gatgttgcta aagataagtt ctccaaggta ctctggtatg ctgttacaaa ctataggacc 900
tggatctttg tccttctcta tggctactcc atgggagttg aattatccac cgacaatgtt 960
atcgccgagt acttctataa caggttcgat ctaaagcttc acacagcagg tgtcattgct 1020
gctacctttg gtatggctaa ccttgtagct cgtccctttg gtggatattt ttctgatgta 1080
gcagcaaggt acttcgggat gagaggcagg ttatgggtgc tctggatttt acagacactt 1140
ggaggagttt tctgtacttg gctcggtcga gctaattcac ttccccttgc tgtcaccgct 1200
atgattctct tctctattgg agcgcaagcc gcatgtggag caacttttgg tatcattccc 1260
tttatttctc gacgatcatt gggcatcata tccggcctaa ctggtgcagg tggaaatttt 1320
gggtccggat tgacacaact agtattcttt tcgagctcaa gtttgtccac agctgcaggt 1380
ctatcctgga tgggtgtcat gatttgcggc tgcactctcc ctgtgacatt ggtttacttc 1440
ccacaatggg gcggcatgtt ctttccgcct tctaaagacg tagtgaagtc aacagaagaa 1500
tcctattatg catcagagtg ggatgaggac gagaagcaaa ggggcatgca ccagaaaagc 1560
ctcaagtttg cggagaacag ccgatctgaa cgtggcaagc gcattgcctc tgcaccaaca 1620
ccaccaagta ccacaccaaa ccgtgtgtag atcaaagcgt tggccatgct tctatagtgc 1680
<210>98
<211>529
<212>PRT
<213>Populus tremula x Populus tremuloides
<400>98
Met Ala Asp Val Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Ser Phe Ala Phe Ser Val Ala Ser Pro Thr Val Pro
20 25 30
Thr Asp Thr Thr Ala Lys Phe Ala Leu Pro Val Asp Ser Glu His Lys
35 40 45
Ala Lys Val Phe Lys Ile Phe Ser Phe Ala Asn Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Ser Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Asp Ser Ala Gly Gly Tyr Leu Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Val Met Pro Leu
195 200 205
Val Tyr Glu Leu Ile Lys Arg Ala Gly Ser Thr Ser Phe Ser Ala Trp
210 215 220
Arg Ile Ala Phe Phe Val Pro Gly Trp Leu His Val Ile Met Gly Ile
225 230 235 240
Leu Val Leu Asn Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Gly Ala
245 250 255
Leu Lys Lys Lys Gly Asp Val Ala Lys Asp Lys Phe Ser Lys Val Leu
260 265 270
Trp Tyr Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Tyr Asn Arg Phe Asp Leu Lys Leu His Thr Ala Gly Val Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Val Ala Arg Pro Phe Gly Gly
325 330 335
Tyr Phe Ser Asp Val Ala Ala Arg Tyr Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Val Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Thr Trp
355 360 365
Leu Gly Arg Ala Asn Ser Leu Pro Leu Ala Val Thr Ala Met Ile Leu
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Ser Ser Ser Leu Ser Thr Ala Ala Gly Leu Ser Trp Met Gly Val Met
435 440 445
Ile Cys Gly Cys Thr Leu Pro Val Thr Leu Val Tyr Phe Pro Gln Trp
450 455 460
Gly Gly Met Phe Phe Pro Pro Ser Lys Asp Val Val Lys Ser Thr Glu
465 470 475 480
Glu Ser Tyr Tyr Ala Ser Glu Trp Asp Glu Asp Glu Lys Gln Arg Gly
485 490 495
Met His Gln Lys Ser Leu Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg
500 505 510
Gly Lys Arg Ile Ala Ser Ala Pro Thr Pro Pro Ser Thr Thr Pro Asn
515 520 525
Arg
<210>99
<211>1611
<212>DNA
＜213〉tobacco
<400>99
ttaaaaaaaa aatgggtgat attgagggcg aaccagggag ttccatgcat ggagtcactg 60
gtagagagcc agttcttgct ttctcagtgg cttctccgat ggtgccaact gataccactg 120
caaaattttc agtaccagtt gatactgaac acaaagccaa gatttttaag ttctattcat 180
tttcaaagcc acatggtctt actttccagc tctcctggat ttctttcttc acttgttttg 240
tttcaacttt tgccgctgcc cctttagttc ctatcattag ggacaacctt aatttaacca 300
aaatggatgt tggtaatgcc ggagttgctt ccgtttccgg aagtatttta tctaggcttg 360
ttatgggtgc agtttgtgat atgttagggc caagatatgg ctgtgcattt ctgattatgt 420
tgtcagcccc aactgtattt tgcatgtcat ttgtgtcgtc ggctggagga tatgttgctg 480
tccggttcat gatcggattc tcgctagcaa catttgtgtc gtgccaatat tggatgagta 540
ctatgtttaa tagtcagatt ataggacttg tcaatgggac agctgcagga tggggaaata 600
tgggtggtgg tgcgactcaa cttattatgc caattgtgta tgatataata agaagagcag 660
gagcaactcc attcactgct tggagaattg catttttcat tcctggctgg cttcatattg 720
tgatgggtat tttggtgttg actcttggcc aagatttgcc tgatgggaac cgtggtgatt 780
tacagaagaa gggtgatgtt tctaaagata aattctccaa catattgtgg tacgctgcaa 840
caaactacag gacgtggatc tttgttcttc tctacggata ctctatggga gttgaactgt 900
ctacagacaa cgtaattgcg gagtacttct tcgacagatt tgatctaaag cttcacacag 960
cgggaattat tgcagcgacc tttggtatgg ctaatttatt agctcgtcca ttcggaggat 1020
tttcttcaga ttacgcagcc aaaagattcg gcatgagagg cagactttgg gtcctttgga 1080
tattacaaac actgggagga gtattctgcg tcctgttggg ccgttcaaat cctctgccca 1140
tagccgtcac attcatgatc cttttctcta ttggtgctca agctgcatgt ggtgcaacat 1200
tcggtattat tccattcatt tctcgccgat ccttaggtat aatctcagga atgactgggg 1260
ctggaggaaa tttcggttct ggattgacac aactgttgtt cttcacgagc tcaaaatact 1320
cgacagcaac agggctaact tacatgggac taatgatcat aggatgcact cttccagtga 1380
ctttctgtca tttcccacaa tggggaagta tgtttttccc accaacgaaa gaccctgtta 1440
agggaagtga ggaacattat tatgccgcag agtataccga agctgagagg cagaaaggta 1500
tgcaccaaaa cagcttgaag ttcgccgaga actgccgatc ggagcgtggc aagcgcgtgg 1560
gatcggcacc tactcctcca aatttaacgc ctaatcgtgt ctgagatccc g 1611
<210>100
<211>530
<212>PRT
＜213〉tobacco
<400>100
Met Gly Asp Ile Glu Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Pro Met Val Pro
20 25 30
Thr Asp Thr Thr Ala Lys Phe Ser Val Pro Val Asp Thr Glu His Lys
35 40 45
Ala Lys Ile Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Val Met Gly Ala Val Cys Asp Met Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Ile
195 200 205
Val Tyr Asp Ile Ile Arg Arg Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Ile Val Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Gly Asp
245 250 255
Leu Gln Lys Lys Gly Asp Val Ser Lys Asp Lys Phe Ser Asn Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ser Ser Asp Tyr Ala Ala Lys Arg Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Val Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Val Leu
355 360 365
Leu Gly Arg Ser Asn Pro Leu Pro Ile Ala Val Thr Phe Met Ile Leu
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Ser Ser Lys Tyr Ser Thr Ala Thr Gly Leu Thr Tyr Met Gly Leu Met
435 440 445
Ile Ile Gly Cys Thr Leu Pro Val Thr Phe Cys His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Phe Pro Pro Thr Lys Asp Pro Val Lys Gly Ser Glu
465 470 475 480
Glu His Tyr Tyr Ala Ala Glu Tyr Thr Glu Ala Glu Arg Gln Lys Gly
485 490 495
Met His Gln Asn Ser Leu Lys Phe Ala Glu Asn Cys Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Gly Ser Ala Pro Thr Pro Pro Asn Leu Thr Pro Asn
515 520 525
Arg Val
530
<210>101
<211>1992
<212>DNA
＜213〉Nicotiana gossei (Nicotiana plumbaginifolia)
<400>101
ttcactttca acatttaatc aatcttagtg ctatcagttt attttcctta agaaaaaaaa 60
tggctgatat tgagggagaa ccagggagtt ctatgcatgg agtcactggt agagagccag 120
ttcttgcttt ctcagtggct tctccaatgg tgccaactga taccactgct aaattttcag 180
taccagttga tactgaacac aaagccaagg tttttaaatt ttattcattt tcaaagcctc 240
atggacttac tttccagctc tcctggattt ctttcttcac ttgttttgta tcaacttttg 300
ctgctgctcc tttagttcct ctcattaggg acaaccttaa tttaaccaaa atggatgttg 360
gtaatgccgg agttgcttcc gtttctggaa gtattttatc taggcttgtt atgggtgcag 420
tttgtgatat gttagggcca agatacggct gtgcatttct gattatgttg tcagccccaa 480
ctgtattttg tatgtcgttc gtgtcgtcgg ctggaggata tgttgctgtc cggttcatga 540
tcggattctc gctcgcaaca tttgtgtcgt gccaatattg gatgagtact atgtttaata 600
gtcagattat aggacttgtc aatggaacag ctgctgggtg gggaaatatg ggtggtggtg 660
cgactcaact tattatgcca attctgtatg atataataag aaaagcagga gcaactccat 720
tcactgcttg gagaattgca tttttcattc ctggctggct tcatgttgta atgggtattt 780
tggtgttgac tcttggtcaa gatttgcctg atggaaaccg tggtgattta cagaagaagg 840
gtggtgtttc taaagataaa ttcaccaaca tattgtggta cgctgcaaca aactacagga 900
cgtggatctt tgtccttctc tacggatact ctatgggagt tgaactgtct accgacaacg 960
taattgcaga gtacttcttc gacagatttg atctaaagct tcacacagcg ggaatcatcg 1020
cagccacatt tggtatggca aatttattag ctcgtccatt cggagggttt tcttcagatt 1080
tcgcagccaa aagattcggc atgaggggta gactttgggt cctttggata ttacaaacac 1140
ttggaggagt attctgtgtc ctgttgggcc gttcaaattc tctgcccata gctgtcacat 1200
tcatgatcct tttctcagtt ggtgctcaag ctgcatgtgg tgcaacattc ggtattattc 1260
ccttcatatc tcgtcgatca ttaggtataa tctcgggaat gactggagct ggaggcaatt 1320
tcggttctgg attgacacaa ctgctgtttt tcacaagctc aaaatactcg acagcaacag 1380
ggctaactta tatggggatg atgatcattg gatgcactct tcctgtgaca ctatgtcatt 1440
tcccacaatg gggaagcatg tttttcccac caacaaaaga tccagttaag ggaagtgagg 1500
aacattatta tgccgcagag tatacagaag ctgagaggca gaaaggtatg catcaaaaca 1560
gcttgaaatt cgccgagaac tgtcgatcgg agcgtggcaa gcgcgtggga tcggcaccta 1620
ctcctccaaa tttaacgcct aaccgtgtct gagtttctca gatttgataa ttttctctaa 1680
ggggatggat cattgtcaat ctctgattgg atgaagattc aatgagctga gtctcaataa 1740
gcatattatt tttaaagaat gtatctttat ttttcttgtc ctttcttctt cttcttcttc 1800
tttttcttgg aatctactta taagttttat gttttttttt ttaaatgaag tatatatatg 1860
acgcattgtg tatctttcct cttgcttttt acatctgaat attcgtatga gccaatgggt 1920
tgttgtagaa tcaaaagtaa tattaataaa acttttgact tccaaaaaaa aaaaaaaaaa 1980
aaaaaaaaaa aa 1992
<210>102
<211>530
<212>PRT
＜213〉Nicotiana gossei
<400>102
Met Ala Asp Ile Glu Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Pro Met Val Pro
20 25 30
Thr Asp Thr Thr Ala Lys Phe Ser Val Pro Val Asp Thr Glu His Lys
35 40 45
Ala Lys Val Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Leu Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Val Met Gly Ala Val Cys Asp Met Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Ile
195 200 205
Leu Tyr Asp Ile Ile Arg Lys Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Val Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Gly Asp
245 250 255
Leu Gln Lys Lys Gly Gly Val Ser Lys Asp Lys Phe Thr Asn Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ser Ser Asp Phe Ala Ala Lys Arg Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Val Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Val Leu
355 360 365
Leu Gly Arg Ser Asn Ser Leu Pro Ile Ala Val Thr Phe Met Ile Leu
370 375 380
Phe Ser Val Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Ser Ser Lys Tyr Ser Thr Ala Thr Gly Leu Thr Tyr Met Gly Met Met
435 440 445
Ile Ile Gly Cys Thr Leu Pro Val Thr Leu Cys His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Phe Pro Pro Thr Lys Asp Pro Val Lys Gly Ser Glu
465 470 475 480
Glu His Tyr Tyr Ala Ala Glu Tyr Thr Glu Ala Glu Arg Gln Lys Gly
485 490 495
Met His Gln Asn Ser Leu Lys Phe Ala Glu Asn Cys Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Gly Ser Ala Pro Thr Pro Pro Asn Leu Thr Pro Asn
515 520 525
Arg Val
530
<210>103
<211>2060
<212>DNA
＜213〉tomato
<400>103
tattctcaat acatttcaaa tcaatcattt ataaaattaa ccagttattt cctcaattga 60
agaaatggct gatgtagaag gatcaccggg gagttctatg catggagtca ccggaagaga 120
acctgttctc gctttctccg tggcttctcc aatggtgcct acggatacct ccgccaaatt 180
ttcagtaccg gtggacactg aacacaaggc taaacaattt aagttttatt cgttttcgaa 240
gcctcatgga cttacgttcc agctctcctg gatctccttt ttcacttgtt tcgtttcgac 300
ttttgctgct gcacctttag ttcctattat tagggacaat cttaatttga caaaaatgga 360
tgttggtaac gctggggttg cttccgtatc cggaagtatt ttatctaggc ttacgatggg 420
tgcggtttgt gatttgttgg gtccaaggta tgggtgcgct tttcttatca tgttgtcagc 480
gccaactgtt ttttgtatgt cttttgtttc atccgctggt ggctacgtag ctgtccggtt 540
catgattggg ttttcgctcg caacgtttgt gtcttgtcaa tattggatga gtactatgtt 600
taatagtaag atcatagggc tagtgaacgg aacggctgct ggatggggta atatgggtgg 660
aggtgcaact caactcatta tgccactttt gtatgatata attcgaaggg cgggtgcaac 720
tccgttcact gcttggagaa ttgcattttt tattcctgga tggcttcatg tggtgatggg 780
tattttagtg ttgactcttg gccaagattt acccgacgga aatcgtggca ctttacagaa 840
gacgggtact gttgctaaag ataaattcgg taacatattg tggtatgctg caacaaacta 900
caggacatgg atctttgttc ttctctatgg atactctatg ggagttgaac tgtcaacaga 960
caacgtcatt gctgagtact tcttcgacag atttgatcta aagcttagca cagcggggat 1020
cattgctgcc acatttggta tggctaacct tttggctcga ccatttggag gattttcttc 1080
tgattacgca gcaaagaaat tcggtatgag agggagactt tgggttttgt ggattttaca 1140
aacacttgga ggagtatttt gtgttctttt gggtcgttcg aattctctac cacttgcggt 1200
aacctttatg atccttttct caatcggagc tcaagctgct tgtggtgcaa cttttggtat 1260
tattccattc atttctcgac gatcgttagg aattataagc ggaatgacag gggcaggtgg 1320
aaattttggt tctggattga ctcaattgtt gtttttcacg agctcaaagt actcgacagc 1380
gacagggtta acttacatgg gattcatgat cataggatgc actcttcctg ttacattttg 1440
tcatttccca caatggggaa gcatgttttt gccaccaaca aaagatccag tcaagggaac 1500
ggaagaacat tattatactt cagagtacac agaggccgag aggcaaaaag ggatgcacca 1560
aaacagcttg aaattcgctg aaaattgccg atcagagcgt ggtaagcgtg ttggttccgc 1620
accaacccca ccaaatttga caccaaatcg tgtttgatga tctttatgag gaatggatag 1680
tcttgaatct gtgatttaaa tttaaggttc aatgtgctga gtcgtctcaa taagcaaaat 1740
ctatcttgat ttttcttctt tgtttttttt ttataatgat attgcttgtt gatctttcca 1800
gacaaatacc ttgaatccac gaaggtgtat gctttttttt taatgaagta tatataatat 1860
attactcatt gtgtatgttt tctattgctt ttttcaaaag aatattctat ggccaatggt 1920
ggttgtgttt tactctgtag attcaaaagt gtattataat aaaactcttg acttgtaaga 1980
aggggactga tcatttattc cagttgattt atagaaagtt cgtgaaaaaa aaaaaaaaaa 2040
aaaaaaaaaa aaaaaaaaaa 2060
<210>104
<211>530
<212>PRT
＜213〉tomato
<400>104
Met Ala Asp Val Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Pro Met Val Pro
20 25 30
Thr Asp Thr Ser Ala Lys Phe Ser Val Pro Val Asp Thr Glu His Lys
35 40 45
Ala Lys Gln Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Thr Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys G1n Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu
195 200 205
Leu Tyr Asp Ile Ile Arg Arg Ala Gly Ala Thr Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu Hi s Val Val Met GlyIle
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Gly Thr
245 250 255
Leu Gln Lys Thr Gly Thr Val Ala Lys Asp Lys Phe Gly Asn Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu Ser Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ser Ser Asp Tyr Ala Ala Lys Lys Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Val Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Val Leu
355 360 365
Leu Gly Arg Ser Asn Ser Leu Pro Leu Ala Val Thr Phe Met Ile Leu
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Ser Ser Lys Tyr Ser Thr Ala Thr Gly Leu Thr Tyr Met Gly Phe Met
435 440 445
Ile Ile Gly Cys Thr Leu Pro Val Thr Phe Cys His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Thr Lys Asp Pro Val Lys Gly Thr Glu
465 470 475 480
Glu His Tyr Tyr Thr Ser Glu Tyr Thr Glu Ala Glu Arg Gln Lys Gly
485 490 495
Met His Gln Asn Ser Leu Lys Phe Ala Glu Asn Cys Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Gly Ser Ala Pro Thr Pro Pro Asn Leu Thr Pro Asn
515 520 525
Arg Val
530
<210>105
<211>1902
<212>DNA
＜213〉Arabidopis thaliana
<400>105
agtgtgagta atttagcttg aatcaaatct caaacttgca aagaaacttg aaatatttta 60
taacaatggg tgattctact ggtgagccgg ggagctccat gcatggagtc accggtagag 120
aacaaagctt tgcattctcg gtgcaatcac caattgtgca taccgacaag acggccaagt 180
tcgaccttcc ggtggacaca gagcataagg caacggtttt caagctcttc tccttcgcca 240
aacctcacat gagaacgttc catctctcgt ggatctcttt ctccacatgt tttgtctcga 300
ctttcgcagc tgcaccactt gtccctatca tccgggagaa tctcaacctc accaaacaag 360
acattggaaa cgccggagtt gcctctgtct ctgggagtat cttctctagg ctcgtgatgg 420
gagccgtgtg tgatcttttg ggtccccgtt acggttgtgc cttccttgtg atgttgtctg 480
ccccaacggt gttctccatg agcttcgtga gtgacgcagc aggcttcata acggtgaggt 540
tcatgattgg tttttgcctg gcgacgtttg tgtcttgtca atactggatg agcactatgt 600
tcaacagtca gatcattggt ctggtgaatg ggacagcagc cggatgggga aacatgggtg 660
gcggcataac gcagttgctc atgcccattg tgtatgaaat cattaggcgc tgcggttcca 720
cagccttcac ggcctggagg atcgccttct ttgtacccgg ttggttgcac atcatcatgg 780
gaatcttggt gctcaatcta ggtcaagatc tgccagatgg aaatcgagct accttggaga 840
aagcgggaga agttgccaaa gacaaattcg gaaagattct gtggtatgcc gttacaaact 900
acaggacttg gatcttcgtt cttctctacg gatactccat gggagttgag ttgagcactg 960
ataatgttat cgccgagtac ttctttgaca ggtttcactt gaagctccac acagcagggc 1020
tcatagcagc atgtttcgga atggccaatt tctttgctcg tccagcagga ggctacgcat 1080
ctgactttgc agccaagtac ttcgggatga gagggaggtt gtggacgttg tggatcatac 1140
agacggctgg tggcctcttc tgtgtgtggc tcggccgcgc caacaccctt gtaactgccg 1200
ttgtggctat ggtgctcttc tctatggggg cacaagctgc ttgcggagcc acctttgcaa 1260
ttgtgccctt tgtctcccgg cgagctctag gcatcatctc gggtttaacc ggggctggag 1320
ggaactttgg atcagggctc acacaactcc tcttcttctc gacctcacac ttcacaactg 1380
aacaagggct aacgtggatg ggagtgatga tagtcgcttg cacgttacct gtgaccttag 1440
ttcactttcc tcaatgggga agcatgttct tgcctccttc cacagatcca gtgaaaggta 1500
cagaggctca ttattatggt tctgagtgga atgagcagga gaagcagaag aacatgcatc 1560
aaggaagcct ccggtttgcc gagaacgcca agtcagaggg tggacgccgc gtccgctctg 1620
ctgctacgcc gcctgagaac acacccaaca atgtttgatc atacattcca cccacggtgg 1680
aatggtgaag gatgatcgca tataagaata tgtcacacag tgaaaaaaaa aaatgcaaat 1740
gttatcaatg cttgcataac attactatct atctttcatt tactaaacaa accttttgct 1800
ttttgccttg aaatcttttt attatatatc aaaatatatc tctatgtctt gaggtttgat 1860
tattttgcat atatcattaa tgatttgata atattggaac tg 1902
<210>106
<211>530
<212>PRT
＜213〉Arabidopis thaliana
<400>106
Met Gly Asp Ser Thr Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Ser Phe Ala Phe Ser Val Gln Ser Pro Ile Val His
20 25 30
Thr Asp Lys Thr Ala Lys Phe Asp Leu Pro Val Asp Thr Glu His Lys
35 40 45
Ala Thr Val Phe Lys Leu Phe Ser Phe Ala Lys Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Ser Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Glu Asn Leu Asn Leu Thr
85 90 95
Lys Gln Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
l00 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Val Met Leu Ser Ala Pro Thr Val Phe Ser
130 135 140
Met Ser Phe Val Ser Asp Ala Ala Gly Phe Ile Thr Val Arg Phe Met
145 150 155 160
Ile Gly Phe Cys Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ile Thr Gln Leu Leu Met Pro Ile
195 200 205
Val Tyr Glu Ile Ile Arg Arg Cys Gly Ser Thr Ala Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Val Pro Gly Trp Leu His Ile Ile Met Gly Ile
225 230 235 240
Leu Val Leu Asn Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Ala Thr
245 250 255
Leu Glu Lys Ala Gly Glu Val Ala Lys Asp Lys Phe Gly Lys Ile Leu
260 265 270
Trp Tyr Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe His Leu Lys Leu His Thr Ala Gly Leu Ile
305 310 315 320
Ala Ala Cys Phe Gly Met Ala Asn Phe Phe Ala Arg Pro Ala Gly Gly
325 330 335
Tyr Ala Ser Asp Phe Ala Ala Lys Tyr Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Ile Gln Thr Ala Gly Gly Leu Phe Cys Val Trp
355 360 365
Leu Gly Arg Ala Asn Thr Leu Val Thr Ala Val Val Ala Met Val Leu
370 375 380
Phe Ser Met Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Ala Ile Val
385 390 395 400
Pro Phe Val Ser Arg Arg Ala Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Ser
420 425 430
Thr Ser His Phe Thr Thr Glu Gln Gly Leu Thr Trp Met Gly Val Met
435 440 445
Ile Val Ala Cys Thr Leu Pro Val Thr Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Thr Asp Pro Val Lys Gly Thr Glu
465 470 475 480
Ala His Tyr Tyr Gly Ser Glu Trp Asn Glu Gln Glu Lys Gln Lys Asn
485 490 495
Met His Gln Gly Ser Leu Arg Phe Ala Glu Asn Ala Lys Ser Glu Gly
500 505 510
Gly Arg Arg Val Arg Ser Ala Ala Thr Pro Pro Glu Asn Thr Pro Asn
515 520 525
Asn Val
530
<210>107
<211>1870
<212>DNA
＜213〉tomato
<400>107
tttcaaatca atcatttata aaattaacca gttatttcct caattgaaga aatgactgat 60
gtagaaggat caccggggag ttctatgcat ggagtcaccg gaagagaacc tgttctcgct 120
ttctccgtgg cttctccaat ggtgcctacg gatacctccg ccaagttttc agtaccggtg 180
gatactgaac acaaggctaa acaatttaag ttttattcgt tttcgaagcc tcatggactt 240
acgttccagc tctcctggat ctcctttttc acttgtttcg tttcgacttt tgctgctgca 300
cctttagttc ctattattag ggacaatctt aatttgacaa aaatggatgt cggtaacgct 360
ggggttgctt ccgtatccgg aagtatttta tctaggctta cgatgggtgc ggtttgtgat 420
ttgttgggtc caaggtatgg gtgcgctttt cttatcatgt tgtcggcccc aactgttttt 480
tgtatgtcct ttgtttcatc cgctggtggc tacgtagccg tccggttcat gattgggttt 540
tccctcgcaa cgtttgtgtc gtgtcaatat tggatgagta ctatgtttaa tagtaagatc 600
atagggctag tgaacggaac ggctgctgga tggggtaata tgggtggagg tgcaactcaa 660
ctcattatgc cacttttgta tgatataatt cggagggcgg gtgcaattcc gttcactgct 720
tggagaattg cattttttat tcctggatgg cttcatgtgg tgatgggtat tttagtgttg 780
actcttggcc aagatttacc cgacggaaat cgtggcactt tacagaagac gggtactgtt 840
gctaaagata aattcggtaa catattgtgg tatgctgcaa caaactacag gacatggatc 900
tttgttcttc tctatggata ctctatggga gttgaactgt caacagacaa cgtcattgct 960
gagtacttct tcgacagatt tgatctaaag cttagcacag cggggatcat tgctgccaca 1020
tttggtatgg ctaacctttt ggctcgacca tttggaggat tttcttctga ttacgcagca 1080
aagaaattcg gtatgagagg gagactttgg gttttgtgga ttttacaaac acttggagga 1140
gtattttgtg ttcttttggg tcgttcgaat tctctaccac ttgctgtaac ctttatgatc 1200
cttttctcaa tcggagctca agctgcttgt ggtgcaactt ttggtattat tccattcatt 1260
tctcgacgat cgttaggaat tataagcgga atgacagggg caggtggaaa ttttggttct 1320
ggattgactc aattgttgtt tttcacgagc tcaaagtact cgacagcgac agggttaact 1380
tacatgggat tcatgatcat aggatgcact cttcctgtta cattttgtca tttcccacaa 1440
tggggaagca tgtttctccc accaacaaaa gatccagtca agggaacgga agaacattat 1500
tatacttcag agtacacaga ggcagagagg caaaaaggga tgcaccaaaa cagcttgaaa 1560
ttcgctgaaa attgccgatc ggagcgtggt aagcgcgttg gttccgcacc cactccacca 1620
aatttgacac caaaccgtgt ctgatcggat tgatgatcgt caatctttag ttcaatgagc 1680
tgagttgttt caataagcaa aataagtcct gatttttttt tcttctttct tttttgtttt 1740
tttcttaaat gatgttgctt gtcaatacgc tggaatctac taaggtgtat gtggttttaa 1800
atttacgtat atataatata tatatatata tatattacac attttaatgt taaaaaaaaa 1860
aaaaaaaaaa 1870
<210>108
<211>530
<212>PRT
＜213〉tomato
<400>108
Met Thr Asp Val Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Val Leu Ala Phe Ser Val Ala Ser Pro Met Val Pro
20 25 30
Thr Asp Thr Ser Ala Lys Phe Ser Val Pro Val Asp Thr Glu His Lys
35 40 45
Ala Lys Gln Phe Lys Phe Tyr Ser Phe Ser Lys Pro His Gly Leu Thr
50 55 60
Phe Gln Leu Ser Trp Ile Ser Phe Phe Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Lys Met Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Leu Ser Arg Leu Thr Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Ile Met Leu Ser Ala Pro Thr Val Phe Cys
130 135 140
Met Ser Phe Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met
145 150 155 160
Ile Gly Phe Ser Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Ser Lys Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu
195 200 205
Leu Tyr Asp Ile Ile Arg Arg Ala Gly Ala Ile Pro Phe Thr Ala Trp
210 215 220
Arg Ile Ala Phe Phe Ile Pro Gly Trp Leu His Val Val Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Gly Thr
245 250 255
Leu Gln Lys Thr Gly Thr Val Ala Lys Asp Lys Phe Gly Asn Ile Leu
260 265 270
Trp Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu Ser Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Phe Ser Ser Asp Tyr Ala Ala Lys Lys Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Val Leu Trp Ile Leu Gln Thr Leu Gly Gly Val Phe Cys Val Leu
355 360 365
Leu Gly Arg Ser Asn Ser Leu Pro Leu Ala Val Thr Phe Met Ile Leu
370 375 380
Phe Ser Ile Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Gly Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Leu Phe Phe Thr
420 425 430
Ser Ser Lys Tyr Ser Thr Ala Thr Gly Leu Thr Tyr Met Gly Phe Met
435 440 445
Ile Ile Gly Cys Thr Leu Pro Val Thr Phe Cys His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Thr Lys Asp Pro Val Lys Gly Thr Glu
465 470 475 480
Glu His Tyr Tyr Thr Ser Glu Tyr Thr Glu Ala Glu Arg Gln Lys Gly
485 490 495
Met His Gln Asn Ser Leu Lys Phe Ala Glu Asn Cys Arg Ser Glu Arg
500 505 510
Gly Lys Arg Val Gly Ser Ala Pro Thr Pro Pro Asn Leu Thr Pro Asn
515 520 525
Arg Val
530
<210>109
<211>1832
<212>DNA
＜213〉colea (Brassica napus)
<400>109
cacattcttg tgaaagaact taaacatttt gcatcaatgg gtgattctac tggcgagccg 60
ggaagctcca tgcatggagt gaccggtaga gaacaaacat ttgctttctc ggtggcttca 120
ccgattgtcc caaccgacaa gacagcaaag ttcgacctgc cggtggactc ggagcataag 180
gcaacggttt tcaagctctt ctccttcgcc aaacctcaca tgcgaacgtt ccatctctcg 240
tggatctctt tctccacgtg ttttgtctcg acgtttgcgg ctgcaccact tgtccccatc 300
atccgagaga atctcaacct cacgaaacaa gacatcggta acgcaggagt tgcgtccgtc 360
tcggggagta tcttctctag gctggtgatg ggagcagtgt gtgatcttct cggtccacgt 420
tacggctgtg ccttccttgt tatgctttcc gcaccaacgg tgttctcaat gagcttcgtg 480
agtggagccg caggattcat aacggtgagg ttcatgattg ggttctgtct agcaacgttt 540
gtgtcttgtc aatatggaat gagtactatg ttcaatagtc agatcatcgg tctggtgaac 600
ggtacagccg ccgggtgggg aaacatgggt ggtggcataa cgcagttgct catgccggtt 660
gtctatgaga tcataaggcg ctgtggtgca acagcgttca cggcctggag gcttgccttc 720
ttcgtccctg gttggttgca catcatcatg ggagtcttgg tgctcaatct aggtcaagat 780
ttgccagatg gtaaccgaag tgccttggag aaaaagggag aagttgccaa agacaaattc 840
ggaaagatta tgtggtacgc cgtcacaaac tacaggactt ggatctttgt tcttctctat 900
ggatactcca tgggagttga gctgagcact gacaatgtta tcgccgaata cttctttgac 960
aggttccact tgaagcttca cacagctgga atcatagcag catgtttcgg aatggccaac 1020
ttctttgctc gtccagcagg aggctatgca tctgaccttg cagccaaata cttcgggatg 1080
agagggaggt tatgggcgtt gtggatcatt cagacagcag gtggtgtctt ctgcgtgtgg 1140
ctcggccgtg ccaacaccct cgttactgcc gttgtagcta tggtcctctt ctctttagga 1200
gcacaagccg cttgtggagc caccttcgca atcgttccct ttgtttctcg gcgagccctt 1260
ggtatcatct caggtttaac cggtgctgga ggaaactttg ggtcagggct cacacagctc 1320
atcttcttct cgacctcaag gttcacaacc gaacaagggc taacatggat gggagtgatg 1380
atagttgcct gcacgttgcc tgtgacttta atccacttcc ctcagtgggg aagcatgttc 1440
ttgcctcctt ctaccgatcc agtcaaaggt ccgaaggagc actattatgc ttcagagtgg 1500
aatgagcagg agaaggagaa gaacatgcat cagggaagcc tcaggtttgc taagaacgcc 1560
aagtctgagg gcggccgccg tgtccgttct gctgctaccc cgcctgagaa cacaccaaac 1620
aatgtttgat cataccagcc acaaggaaag tgtgaaggat ggtcgcagat aagaatttat 1680
atgtcccaca gtgaaaacaa atgcgtatgt tatcaatgct tgctggacgt tatttgttgt 1740
gtatctttct ttttttcact gaagaaacat ttgttttgtt tacggcttca agaaatattt 1800
ttctaatcaa aatgtctacc tcttgcgaca tt 1832
<210>110
<211>530
<212>PRT
＜213〉colea
<400>110
Met Gly Asp Ser Thr Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Thr Phe Ala Phe Ser Val Ala Ser Pro Ile Val Pro
20 25 30
Thr Asp Lys Thr Ala Lys Phe Asp Leu Pro Val Asp Ser Glu His Lys
35 40 45
Ala Thr Val Phe Lys Leu Phe Ser Phe Ala Lys Pro His Met Arg Thr
50 55 60
Phe His Leu Ser Trp Ile Ser Phe Ser Thr Cys Phe Val Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Glu Asn Leu Asn Leu Thr
85 90 95
Lys Gln Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Val Met Leu Ser Ala Pro Thr Val Phe Ser
130 135 140
Met Ser Phe Val Ser Gly Ala Ala Gly Phe Ile Thr Val Arg Phe Met
145 150 155 160
Ile Gly Phe Cys Leu Ala Thr Phe Val Ser Cys Gln Tyr Gly Met Ser
165 170 175
Thr Met Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Ile Thr Gln Leu Leu Met Pro Val
195 200 205
Val Tyr Glu Ile Ile Arg Arg Cys Gly Ala Thr Ala Phe Thr Ala Trp
210 215 220
Arg Leu Ala Phe Phe Val Pro Gly Trp Leu His Ile Ile Met Gly Val
225 230 235 240
Leu Val Leu Asn Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Ser Ala
245 250 255
Leu Glu Lys Lys Gly Glu Val Ala Lys Asp Lys Phe Gly Lys Ile Met
260 265 270
Trp Tyr Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe His Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Cys Phe Gly Met Ala Asn Phe Phe Ala Arg Pro Ala Gly Gly
325 330 335
Tyr Ala Ser Asp Leu Ala Ala Lys Tyr Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Ala Leu Trp Ile Ile Gln Thr Ala Gly Gly Val Phe Cys Val Trp
355 360 365
Leu Gly Arg Ala Asn Thr Leu Val Thr Ala Val Val Ala Met Val Leu
370 375 380
Phe Ser Leu Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Ala Ile Val
385 390 395 400
Pro Phe Val Ser Arg Arg Ala Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Ile Phe Phe Ser
420 425 430
Thr Ser Arg Phe Thr Thr Glu Gln Gly Leu Thr Trp Met Gly Val Met
435 440 445
Ile Val Ala Cys Thr Leu Pro Val Thr Leu Ile His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Pro Ser Thr Asp Pro Val Lys Gly Pro Lys
465 470 475 480
Glu His Tyr Tyr Ala Ser Glu Trp Asn Glu Gln Glu Lys Glu Lys Asn
485 490 495
Met His Gln Gly Ser Leu Arg Phe Ala Lys Asn Ala Lys Ser Glu Gly
500 505 510
Gly Arg Arg Val Arg Ser Ala Ala Thr Pro Pro Glu Asn Thr Pro Asn
515 520 525
Asn Val
530
<210>111
<211>1584
<212>DNA
＜213〉Arabidopis thaliana
<400>111
atggccgatg gttttggtga accgggaagc tcaatgcatg gagtcaccgg cagagaacaa 60
agctatgcat tctctgtcga gtctccggca gttccttccg actcatcagc aaaattttct 120
ctccccgtgg acaccgaaca caaagccaaa gtcttcaaac tcttatcctt tgaagctcca 180
catatgagaa ctttccatct tgcttggatc tcattcttca cttgcttcat ttccactttc 240
gctgctgctc ctcttgtccc catcattaga gataacctta atctcacaag acaagatgtc 300
ggaaatgctg gtgttgcttc tgtctctggc agtatcttct ctaggcttgt tatgggagca 360
gtttgtgatc tccttgggcc acgttatggc tgtgctttcc tcgtcatgct ctctgctcca 420
accgtcttct ccatgtcttt cgttggtggt gccggagggt acataacggt gaggttcatg 480
atcgggttct gcctggcgac tttcgtgtca tgccagtatt ggatgagcac aatgttcaat 540
ggtcagatca taggtctagt gaacgggaca gcggcagggt gggggaacat gggcggtggg 600
gtcactcagt tgctcatgcc aatggtctat gagatcatcc gacggttagg gtccacgtcc 660
ttcaccgcat ggaggatggc tttcttcgtc cccgggtgga tgcacatcat catggggatc 720
ttggtcttga ctctagggca agacctccct gatggtaata gaagcacact cgagaagaaa 780
ggtgcagtta ctaaagacaa gttctcaaag gttttatggt acgcgatcac gaactatagg 840
acatgggttt tcgtgctgct atatggatac tccatgggag tagagctcac aaccgataac 900
gtcatcgctg agtacttttt cgacaggttc catcttaagc ttcataccgc cggtataatc 960
gcggcaagct ttggtatggc aaacttcttt gcccgtccta ttggtggttg ggcctcagat 1020
attgcggcta gacgcttcgg catgagaggc cgtctctgga ccctatggat catccaaacc 1080
ttaggcggtt tcttctgcct atggctaggc cgagccacca cgctcccgac cgcggttgtc 1140
ttcatgatcc tcttctctct cggcgctcaa gccgcttgtg gagctacctt tgctatcata 1200
cctttcatct cacgccgctc cttagggatc atctctggtc ttactggagc tggtggaaac 1260
ttcggctctg gtttgaccca actcgtattc ttctcgacct caacgttctc cacggaacaa 1320
gggctgacat ggatgggggt gatgattatg gcgtgtacat tacccgtcac tttagtgcac 1380
ttcccgcaat ggggaagcat gtttttgcct tccacggaag atgaagtgaa gtctacggag 1440
gagtattatt acatgaaaga gtggacagag accgagaagc gaaagggtat gcatgaaggg 1500
agtttgaagt tcgccgtgaa tagtagatcg gagcgtggac ggcgcgtggc ttctgcaccg 1560
tctcctccgc cggaacacgt ttaa 1584
<210>112
<211>527
<212>PRT
＜213〉Arabidopis thaliana
<400>112
Met Ala Asp Gly Phe Gly Glu Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Gln Ser Tyr Ala Phe Ser Val Glu Ser Pro Ala Val Pro
20 25 30
Ser Asp Ser Ser Ala Lys Phe Ser Leu Pro Val Asp Thr Glu His Lys
35 40 45
Ala Lys Val Phe Lys Leu Leu Ser Phe Glu Ala Pro His Met Arg Thr
50 55 60
Phe His Leu Ala Trp Ile Ser Phe Phe Thr Cys Phe Ile Ser Thr Phe
65 70 75 80
Ala Ala Ala Pro Leu Val Pro Ile Ile Arg Asp Asn Leu Asn Leu Thr
85 90 95
Arg Gln Asp Val Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile
100 105 110
Phe Ser Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg
115 120 125
Tyr Gly Cys Ala Phe Leu Val Met Leu Ser Ala Pro Thr Val Phe Ser
130 135 140
Met Ser Phe Val Gly Gly Ala Gly Gly Tyr Ile Thr Val Arg Phe Met
145 150 155 160
Ile Gly Phe Cys Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser
165 170 175
Thr Met Phe Asn Gly Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala
180 185 190
Gly Trp Gly Asn Met Gly Gly Gly Val Thr Gln Leu Leu Met Pro Met
195 200 205
Val Tyr Glu Ile Ile Arg Arg Leu Gly Ser Thr Ser Phe Thr Ala Trp
210 215 220
Arg Met Ala Phe Phe Val Pro Gly Trp Met His Ile Ile Met Gly Ile
225 230 235 240
Leu Val Leu Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Arg Ser Thr
245 250 255
Leu Glu Lys Lys Gly Ala Val Thr Lys Asp Lys Phe Ser Lys Val Leu
260 265 270
Trp Tyr Ala Ile Thr Asn Tyr Arg Thr Trp Val Phe Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe His Leu Lys Leu His Thr Ala Gly Ile Ile
305 310 315 320
Ala Ala Ser Phe Gly Met Ala Asn Phe Phe Ala Arg Pro Ile Gly Gly
325 330 335
Trp Ala Ser Asp Ile Ala Ala Arg Arg Phe Gly Met Arg Gly Arg Leu
340 345 350
Trp Thr Leu Trp Ile Ile Gln Thr Leu Gly Gly Phe Phe Cys Leu Trp
355 360 365
Leu Gly Arg Ala Thr Thr Leu Pro Thr Ala Val Val Phe Met Ile Leu
370 375 380
Phe Ser Leu Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Ala Ile Ile
385 390 395 400
Pro Phe Ile Ser Arg Arg Ser Leu Gly Ile Ile Ser Gly Leu Thr Gly
405 410 415
Ala Gly Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser
420 425 430
Thr Ser Thr Phe Ser Thr Glu Gln Gly Leu Thr Trp Met Gly Val Met
435 440 445
Ile Met Ala Cys Thr Leu Pro Val Thr Leu Val His Phe Pro Gln Trp
450 455 460
Gly Ser Met Phe Leu Pro Ser Thr Glu Asp Glu Val Lys Ser Thr Glu
465 470 475 480
Glu Tyr Tyr Tyr Met Lys Glu Trp Thr Glu Thr Glu Lys Arg Lys Gly
485 490 495
Met His Glu Gly Ser Leu Lys Phe Ala Val Asn Ser Arg Ser Glu Arg
500 505 510
Gly Arg Arg Val Ala Ser Ala Pro Ser Pro Pro Pro Glu His Val
515 520 525
<210>113
<211>1709
<212>DNA
＜213〉Arabidopis thaliana
<400>113
aaacttgaat tttctcaaag gaacttgata cgtttaaaat acatgggttc tactgatgag 60
cccggaagtt ccatgcatgg agttaccggt agagaacaga gctatgcttt ctcggtagat 120
ggtagtgagc cgaccaacac aaagaaaaag tacaatctgc cggtggacgc ggaggataag 180
gcaacggttt tcaagctctt ctccttcgcc aaacctcaca tgagaacgtt ccacctctcg 240
tggatctctt tctccacatg ttttgtttcg acgttcgcag ctgcaccact tatcccgatc 300
atcagggaga atcttaacct caccaaacat gacattggaa acgctggagt tgcctccgtc 360
tcggggagta tcttctctag gctcgtgatg ggagccgtgt gtgatctttt gggtcctcgt 420
tacggttgtg ccttccttgt gatgttgtct gccccaacgg tgttctccat gagcttcgtg 480
agtgacgcag caggcttcat aacggtgagg ttcatgattg gtttttgcct ggcgacgttt 540
gtgtcttgtc aatactggat gagcactatg ttcaacagtc agatcatcgg tctggtgaac 600
gggacagcag ccggatgggg aaacatgggt ggcggcataa cgcagttgct catgcccatt 660
gtgtatgaaa tcattaggcg ctgcggatca acagcgttca cggcctggag gatcgccttc 720
tttgtccccg gttggttgca catcatcatg ggaatcttgg tgctcacgct aggtcaagat 780
ctgccaggtg gaaacagagc tgccatggag aaagcgggag aagttgccaa agacaaattc 840
ggaaagattc tatggtacgc cgttacaaat tacaggactt ggattttcgt tcttctgtat 900
ggatattcca tgggagttga gttaagcaca gacaatgtta tcgccgagta cttctttgat 960
aggtttcact tgaagcttca cacagcgggg attatagcag catgtttcgg aatggccaat 1020
ttctttgctc gtccagcagg aggctgggca tctgacattg cagccaagcg cttcggaatg 1080
cgagggaggt tgtggacttt gtggatcatt cagacgtccg gtggtctctt ttgtgtgtgg 1140
ctcggacgtg ccaacaccct cgtcactgcc gttgtatcta tggtcctctt ctctttagga 1200
gcacaagccg cttgcggagc cacctttgct atcgtgccct ttgtctcccg gcgagctcta 1260
ggcattatct cgggtttaac cggggctgga gggaactttg ggtcaggact cacacagctc 1320
gtctttttct cgacttcgcg cttcacaact gaagaagggc taacgtggat gggagtgatg 1380
atagttgctt gcacgttgcc tgttacctta atccactttc ctcagtgggg aagcatgttc 1440
ttccctcctt ccaacgattc ggtcgacgct acggagcact attatgttgg cgaatatagt 1500
aaggaggagc agcagattgg catgcattta aaaagcaaac tgtttgctga tggagccaag 1560
accgagggag gcagcagcgt ccacaaaggg aacgcaacca acaatgcttg atcatgtgtc 1620
attgatatca agaaattaat aatttcactt atgtgaaatg gacataaact gttggaaaat 1680
aaagaaccat ttctttcatc atttgcttt 1709
<210>114
<211>522
<212>PRT
＜213〉Arabidopis thaliana
<400>114
Met Gly Ser Thr Asp Glu Pro Gly Ser Ser Met His Gly Val Thr Gly
1 5 10 15
Arg Glu Gln Ser Tyr Ala Phe Ser Val Asp Gly Ser Glu Pro Thr Asn
20 25 30
Thr Lys Lys Lys Tyr Asn Leu Pro Val Asp Ala Glu Asp Lys Ala Thr
35 40 45
Val Phe Lys Leu Phe Ser Phe Ala Lys Pro Hi s Met Arg Thr Phe His
50 55 60
Leu Ser Trp Ile Ser Phe Ser Thr Cys Phe Val Ser Thr Phe Ala Ala
65 70 75 80
Ala Pro Leu Ile Pro Ile Ile Arg Glu Asn Leu Asn Leu Thr Lys His
85 90 95
Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser
100 105 110
Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly
115 120 125
Cys Ala Phe Leu Val Met Leu Ser Ala Pro Thr Val Phe Ser Met Ser
130 135 140
Phe Val Ser Asp Ala Ala Gly Phe Ile Thr Val Arg Phe Met Ile Gly
145 150 155 160
Phe Cys Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met
165 170 175
Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala Gly Trp
180 185 190
Gly Asn Met Gly Gly Gly Ile Thr Gln Leu Leu Met Pro Ile Val Tyr
195 200 205
Glu Ile Ile Arg Arg Cys Gly Ser Thr Ala Phe Thr Ala Trp Arg Ile
210 215 220
Ala Phe Phe Val Pro Gly Trp Leu His Ile Ile Met Gly Ile Leu Val
225 230 235 240
Leu Thr Leu Gly Gln Asp Leu Pro Gly Gly Asn Arg Ala Ala Met Glu
245 250 255
Lys Ala Gly Glu Val Ala Lys Asp Lys Phe Gly Lys Ile Leu Trp Tyr
260 265 270
Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr
275 280 285
Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu Tyr Phe
290 295 300
Phe Asp Arg Phe His Leu Lys Leu His Thr Ala Gly Ile Ile Ala Ala
305 310 315 320
Cys Phe Gly Met Ala Asn Phe Phe Ala Arg Pro Ala Gly Gly Trp Ala
325 330 335
Ser Asp Ile Ala Ala Lys Arg Phe Gly Met Arg Gly Arg Leu Trp Thr
340 345 350
Leu Trp Ile Ile Gln Thr Ser Gly Gly Leu Phe Cys Val Trp Leu Gly
355 360 365
Arg Ala Asn Thr Leu Val Thr Ala Val Val Ser Met Val Leu Phe Ser
370 375 380
Leu Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Ala Ile Val Pro Phe
385 390 395 400
Val Ser Arg Arg Ala Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly
405 410 415
Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser Thr Ser
420 425 430
Arg Phe Thr Thr Glu Glu Gly Leu Thr Trp Met Gly Val Met Ile Val
435 440 445
Ala Cys Thr Leu Pro Val Thr Leu Ile His Phe Pro Gln Trp Gly Ser
450 455 460
Met Phe Phe Pro Pro Ser Asn Asp Ser Val Asp Ala Thr Glu His Tyr
465 470 475 480
Tyr Val Gly Glu Tyr Ser Lys Glu Glu Gln Gln Ile Gly Met His Leu
485 490 495
Lys Ser Lys Leu Phe Ala Asp Gly Ala Lys Thr Glu Gly Gly Ser Ser
500 505 510
Val His Lys Gly Asn Ala Thr Asn Asn Ala
515 520
<210>115
<211>1709
<212>DNA
＜213〉Arabidopis thaliana
<400>115
aaacttgaat tttctcaaag gaacttgata cgtttaaaat acatgggttc tactgatgag 60
cccagaagtt ccatgcatgg agttaccggt agagaacaga gctatgcttt ctcggtagat 120
ggtagtgagc cgaccaacac aaagaaaaag tacaatctgc cggtggacgc ggaggataag 180
gcaacggttt tcaagctctt ctccttcgcc aaacctcaca tgagaacgtt ccacctctcg 240
tggatctctt tctccacatg ttttgtttcg acgttcgcag ctgcaccact tatcccgatc 300
atcagggaga atcttaacct caccaaacat gacattggaa acgctggagt tgcctccgtc 360
tcggggagta tcttctctag gctcgtgatg ggagccgtgt gtgatctttt gggtcctcgt 420
tacggttgtg ccttccttgt gatgttgtct gccccaacgg tgttctccat gagcttcgtg 480
agtgacgcag caggcttcat aacggtgagg ttcatgattg gtttttgcct ggcgacgttt 540
gtgtcttgtc aatactggat gagcactatg ttcaacagtc agatcatcgg tctggtgaac 600
gggacagcag ccggatgggg aaacatgggt ggcggcataa cgcagttgct catgcccatt 660
gtgtatgaaa tcattaggcg ctgcggatca acagcgttca cggcctggag gatcgccttc 720
tttgtccccg gttggttgca catcatcatg ggaatcttgg tgctcacgct aggtcaagat 780
ctgccaggtg gaaacagagc tgccatggag aaagcgggag aagttgccaa agacaaattc 840
ggaaagattc tatggtacgc cgttacaaat tacaggactt ggattttcgt tcttctgtat 900
ggatattcca tgggagttga gttaagcaca gacaatgtta tcgccgagta cttctttgac 960
aggtttcact tgaagcttca cacagcgggg attatagcag catgtttcgg aatggccaat 1020
ttctttgctc gtccagcagg aggctgggca tctgacattg cagccaagcg cttcggaatg 1080
cgagggaggt tgtggacttt gtggatcatt cagacgtccg gtggtctctt ttgtgtgtgg 1140
ctcggacgtg ccaacaccct cgtcactgcc gttgtatcta tggtcctctt ctctttagga 1200
gcacaagccg cttgcggagc cacctttgct atcgtgccct ttgtctcccg gcgagctcta 1260
ggcattatct cgggtttaac cggggctgga gggaactttg ggtcaggact cacacagctc 1320
gtctttttct cgacttcgcg cttcacaact gaagaagggc taacgtggat gggagtgatg 1380
atagttgctt gcacgttgcc tgttacctta atccactttc ctcagtgggg aagcatgttc 1440
ttccctcctt ccaacgattc ggtcgacgct acggagcact attatgttgg cgaatatagt 1500
aaggaggagc agcagattgg catgcattta aaaagcaaac tgtttgctga tggagccaag 1560
accgagggag gcagcagcgt ccacaaaggg aacgcaacca acaatgcttg atcatgtgtc 1620
attgatatca agaaattaat aatttcactt atgtgaaatg gacataaact gttggaaaat 1680
aaagaaccat ttctttcatc atttgcttt 1709
<210>116
<211>522
<212>PRT
＜213〉Arabidopis thaliana
<400>116
Met Gly Ser Thr Asp Glu Pro Arg Ser Ser Met His Gly Val Thr Gly
1 5 10 15
Arg Glu Gln Ser Tyr Ala Phe Ser Val Asp Gly Ser Glu Pro Thr Asn
20 25 30
Thr Lys Lys Lys Tyr Asn Leu Pro Val Asp Ala Glu Asp Lys Ala Thr
35 40 45
Val Phe Lys Leu Phe Ser Phe Ala Lys Pro His Met Arg Thr Phe His
50 55 60
Leu Ser Trp Ile Ser Phe Ser Thr Cys Phe Val Ser Thr Phe Ala Ala
65 70 75 80
Ala Pro Leu Ile Pro Ile Ile Arg Glu Asn Leu Asn Leu Thr Lys His
85 90 95
Asp Ile Gly Asn Ala Gly Val Ala Ser Val Ser Gly Ser Ile Phe Ser
100 105 110
Arg Leu Val Met Gly Ala Val Cys Asp Leu Leu Gly Pro Arg Tyr Gly
115 120 125
Cys Ala Phe Leu Val Met Leu Ser Ala Pro Thr Val Phe Ser Met Ser
130 135 140
Phe Val Ser Asp Ala Ala Gly Phe Ile Thr Val Arg Phe Met Ile Gly
145 150 155 160
Phe Cys Leu Ala Thr Phe Val Ser Cys Gln Tyr Trp Met Ser Thr Met
165 170 175
Phe Asn Ser Gln Ile Ile Gly Leu Val Asn Gly Thr Ala Ala Gly Trp
180 185 190
Gly Asn Met Gly Gly Gly Ile Thr Gln Leu Leu Met Pro Ile Val Tyr
195 200 205
Glu Ile Ile Arg Arg Cys Gly Ser Thr Ala Phe Thr Ala Trp Arg Ile
210 215 220
Ala Phe Phe Val Pro Gly Trp Leu His Ile Ile Met Gly Ile Leu Val
225 230 235 240
Leu Thr Leu Gly Gln Asp Leu Pro Gly Gly Asn Arg Ala Ala Met Glu
245 250 255
Lys Ala Gly Glu Val Ala Lys Asp Lys Phe Gly Lys Ile Leu Trp Tyr
260 265 270
Ala Val Thr Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr
275 280 285
Ser Met Gly Val Glu Leu Ser Thr Asp Asn Val Ile Ala Glu Tyr Phe
290 295 300
Phe Asp Arg Phe His Leu Lys Leu His Thr Ala Gly Ile Ile Ala Ala
305 310 315 320
Cys Phe Gly Met Ala Asn Phe Phe Ala Arg Pro Ala Gly Gly Trp Ala
325 330 335
Ser Asp Ile Ala Ala Lys Arg Phe Gly Met Arg Gly Arg Leu Trp Thr
340 345 350
Leu Trp Ile Ile Gln Thr Ser Gly Gly Leu Phe Cys Val Trp Leu Gly
355 360 365
Arg Ala Asn Thr Leu Val Thr Ala Val Val Ser Met Val Leu Phe Ser
370 375 380
Leu Gly Ala Gln Ala Ala Cys Gly Ala Thr Phe Ala Ile Val Pro Phe
385 390 395 400
Val Ser Arg Arg Ala Leu Gly Ile Ile Ser Gly Leu Thr Gly Ala Gly
405 410 415
Gly Asn Phe Gly Ser Gly Leu Thr Gln Leu Val Phe Phe Ser Thr Ser
420 425 430
Arg Phe Thr Thr Glu Glu Gly Leu Thr Trp Met Gly Val Met Ile Val
435 440 445
Ala Cys Thr Leu Pro Val Thr Leu Ile His Phe Pro Gln Trp Gly Ser
450 455 460
Met Phe Phe Pro Pro Ser Asn Asp Ser Val Asp Ala Thr Glu His Tyr
465 470 475 480
Tyr Val Gly Glu Tyr Ser Lys Glu Glu Gln Gln Ile Gly Met His Leu
485 490 495
Lys Ser Lys Leu Phe Ala Asp Gly Ala Lys Thr Glu Gly Gly Ser Ser
500 505 510
Val His Lys Gly Asn Ala Thr Asn Asn Ala
515 520
<210>117
<211>1620
<212>DNA
＜213〉Arabidopis thaliana
<400>117
atgactcaca accattctaa tgaagaaggc tccattggaa cctccttgca tggagttaca 60
gcaagagaac aagtcttctc tttctccgtc gatgcttcgt ctcaaacagt ccaatcagac 120
gatccaacag ctaaattcgc ccttccggtt gattccgaac atcgagccaa agtgttcaac 180
ccactctctt ttgctaaacc tcacatgaga gccttccact taggatggct ctcattcttc 240
acatgcttca tctccacctt cgcggcagca ccattagtcc ccatcatccg cgacaacctc 300
gacctcacta aaaccgacat tggaaacgcc ggagtcgcat ccgtctctgg tgccattttc 360
tcaaggttag ccatgggagc ggtttgtgat ctcctcggtg cacgatatgg gactgccttc 420
tccctcatgc taaccgcccc aaccgtcttc tcaatgtcgt ttgtgggtgg ccctagcgga 480
tacttaggcg tccggttcat gatcggattc tgtctcgcca cgtttgtatc atgccagtat 540
tggaccagcg ttatgttcaa cggtaagatc ataggactag tgaacggctg tgcaggcggg 600
tggggtgata tgggcggtgg agtgactcaa ctcctaatgc cgatggtctt ccacgtcatc 660
aaacttgccg gagccactcc gttcatggcc tggcggatag ctttcttcgt tcccggattt 720
cttcaagttg ttatgggcat tctcgtcctc agtctcggcc aagatctccc tgacggtaac 780
ctaagtaccc ttcagaagag tggtcaagtc tctaaagaca aattctccaa ggttttctgg 840
tttgctgtga agaactacag aacatggatt ttattcgttc tttatggatc ttccatggga 900
attgaattaa ctatcaacaa cgttatctcc ggatattttt acgacaggtt taaccttaag 960
cttcaaacag ctggtatagt agcagccagc tttggaatgg ctaacttcat cgcccgtccc 1020
ttcggtggtt acgcttctga tgtagcggct cgggtttttg gcatgagagg ccggttatgg 1080
accttatgga tctttcaaac cgtaggagct cttttctgta tctggctagg tcgagctagt 1140
tcacttccca tagcaatcct agcaatgatg ctcttctcaa tcggtacaca agcagcttgc 1200
ggagccctct tcggagttgc accttttgtc tcgcgccgct ctctagggct catatcggga 1260
ctaaccggcg caggaggaaa cttcgggtcc ggtttgactc aactgctttt cttctcatca 1320
gcgaggttta gtacagctga gggactctca ttgatgggcg ttatggcggt tttgtgcaca 1380
ctcccagttg cgtttataca ttttccgcaa tggggaagca tgtttttaag accgtcgacc 1440
gatggagaaa gatcacagga ggaatattat tacggttctg agtggacgga gaatgagaaa 1500
caacaaggat tgcacgaagg aagcatcaaa tttgcagaga atagtaggtc agagagaggc 1560
cggaaagtag ctttggctaa cattccaacg ccggagaacg gaactccaag tcatgtttga 1620
<210>118
<211>539
<212>PRT
＜213〉Arabidopis thaliana
<400>118
Met Thr His Asn His Ser Asn Glu Glu Gly Ser Ile Gly Thr Ser Leu
1 5 10 15
His Gly Val Thr Ala Arg Glu Gln Val Phe Ser Phe Ser Val Asp Ala
20 25 30
Ser Ser Gln Thr Val Gln Ser Asp Asp Pro Thr Ala Lys Phe Ala Leu
35 40 45
Pro Val Asp Ser Glu His Arg Ala Lys Val Phe Asn Pro Leu Ser Phe
50 55 60
Ala Lys Pro His Met Arg Ala Phe His Leu Gly Trp Leu Ser Phe Phe
65 70 75 80
Thr Cys Phe Ile Ser Thr Phe Ala Ala Ala Pro Leu Val Pro Ile Ile
85 90 95
Arg Asp Asn Leu Asp Leu Thr Lys Thr Asp Ile Gly Asn Ala Gly Val
100 105 110
Ala Ser Val Ser Gly Ala Ile Phe Ser Arg Leu Ala Met Gly Ala Val
115 120 125
Cys Asp Leu Leu Gly Ala Arg Tyr Gly Thr Ala Phe Ser Leu Met Leu
130 135 140
Thr Ala Pro Thr Val Phe Ser Met Ser Phe Val Gly Gly Pro Ser Gly
145 150 155 160
Tyr Leu Gly Val Arg Phe Met Ile Gly Phe Cys Leu Ala Thr Phe Val
165 170 175
Ser Cys Gln Tyr Trp Thr Ser Val Met Phe Asn Gly Lys Ile Ile Gly
180 185 190
Leu Val Asn Gly Cys Ala Gly Gly Trp Gly Asp Met Gly Gly Gly Val
195 200 205
Thr Gln Leu Leu Met Pro Met Val Phe His Val Ile Lys Leu Ala Gly
210 215 220
Ala Thr Pro Phe Met Ala Trp Arg Ile Ala Phe Phe Val Pro Gly Phe
225 230 235 240
Leu Gln Val Val Met Gly Ile Leu Val Leu Ser Leu Gly Gln Asp Leu
245 250 255
Pro Asp Gly Asn Leu Ser Thr Leu Gln Lys Ser Gly Gln Val Ser Lys
260 265 270
Asp Lys Phe Ser Lys Val Phe Trp Phe Ala Val Lys Asn Tyr Arg Thr
275 280 285
Trp Ile Leu Phe Val Leu Tyr Gly Ser Ser Met Gly Ile Glu Leu Thr
290 295 300
Ile Asn Asn Val Ile Ser Gly Tyr Phe Tyr Asp Arg Phe Asn Leu Lys
305 310 315 320
Leu Gln Thr Ala Gly Ile Val Ala Ala Ser Phe Gly Met Ala Asn Phe
325 330 335
Ile Ala Arg Pro Phe Gly Gly Tyr Ala Ser Asp Val Ala Ala Arg Val
340 345 350
Phe Gly Met Arg Gly Arg Leu Trp Thr Leu Trp Ile Phe Gln Thr Val
355 360 365
Gly Ala Leu Phe Cys Ile Trp Leu Gly Arg Ala Ser Ser Leu Pro Ile
370 375 380
Ala Ile Leu Ala Met Met Leu Phe Ser Ile Gly Thr Gln Ala Ala Cys
385 390 395 400
Gly Ala Leu Phe Gly Val Ala Pro Phe Val Ser Arg Arg Ser Leu Gly
405 410 415
Leu Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Phe Gly Ser Gly Leu
420 425 430
Thr Gln Leu Leu Phe Phe Ser Ser Ala Arg Phe Ser Thr Ala Glu Gly
435 440 445
Leu Ser Leu Met Gly Val Met Ala Val Leu Cys Thr Leu Pro Val Ala
450 455 460
Phe Ile His Phe Pro Gln Trp Gly Ser Met Phe Leu Arg Pro Ser Thr
465 470 475 480
Asp Gly Glu Arg Ser Gln Glu Glu Tyr Tyr Tyr Gly Ser Glu Trp Thr
485 490 495
Glu Asn Glu Lys Gln Gln Gly Leu His Glu Gly Ser Ile Lys Phe Ala
500 505 510
Glu Asn Ser Arg Ser Glu Arg Gly Arg Lys Val Ala Leu Ala Asn Ile
515 520 525
Pro Thr Pro Glu Asn Gly Thr Pro Ser His Val
530 535
<210>119
<211>1629
<212>DNA
＜213〉Arabidopis thaliana
<400>119
atggctcaca accattctaa tgaagacggc tctattggaa cctccttgca tggagtcacg 60
gcaagggagc aagtcttctc cttctccgtc caagaagatg tcccttcatc tcaagccgtc 120
cgaacaaacg atccaacggc taagtttgcc ctaccagtgg actccgaaca tagggcaaaa 180
gtgttcaaac cactatcatt cgctaaacca catatgagag ccttccactt aggatggatc 240
tctttcttca cttgcttcat ctccaccttc gcagccgcac ctctagtccc cgtcattcgc 300
gacaatctcg acctgaccaa aaccgacatc ggaaatgctg gagttgcatc agtttccggc 360
gccattttct cgagactcgc tatgggtgct gtatgtgacc ttctaggggc acgttatgga 420
accgccttct cacttatgct tacagctcca gcagttttct ccatgtcgtt cgtagctgac 480
gcgggaagct acttagccgt aaggttcatg atcggttttt gcttagcaac gttcgtatca 540
tgtcagtact ggacgagtgt tatgttcact ggaaagatta tcggactcgt taacggatgt 600
gctggagggt ggggagatat gggaggagga gtgactcagc tactaatgcc aatggtcttc 660
cacgtcatca aactcaccgg agccactccc ttcacggctt ggaggttcgc cttcttcatc 720
cccggcattc ttcagatagt tatgggtatt ctcgttctca ctctcggcca agatcttccc 780
gatggtaacc tcagtactct ccaaaagagt ggtcaagttt ctaaagacaa attctccaag 840
gtcttttggt tcgctgtgaa aaactataga acatggatct tattcatgct ctatggattt 900
tctatgggag ttgaattaac gatcaacaac gttatatctg gatacttcta cgataggttt 960
aaccttacgc ttcacacagc tggtattata gcagccagct ttggtatggc aaacttcttt 1020
gcccgtcctt ttggtggcta cgcttcagat gtagctgcac ggctcttcgg tatgagggga 1080
cggttatgga tcttgtggat cttacaaact gttggagctc tcttttgcat ctggcttggt 1140
cgtgctagtt cactacctat agctatctta gccatgatgc ttttttccat gggcacacaa 1200
gctgcttgtg gagctctctt tggtgttgct ccttttgttt cccgccgttc tcttggactt 1260
atctcgggat taactggtgc tggtggaaat tttgggtcgg gagttactca acttcttttc 1320
ttctcttcct cgaggtttag tacggcggaa ggactatcgt tgatgggcgt tatggctgtt 1380
gtgtgctctc ttccggttgc gtttatacat tttccgcagt ggggaagcat gttcttgagg 1440
ccatcacaag atggagagaa atcaaaggaa gagcattact atggagcgga atggacagag 1500
gaagagaaga gcttaggact acacgaagga agcattaaat ttgctgaaaa cagccggtca 1560
gagagaggcc gcaaggcgat gttggctgat attccaacgc cggaaaccgg atctccggct 1620
catgtctag 1629
<210>120
<211>542
<212>PRT
＜213〉Arabidopis thaliana
<400>120
Met Ala His Asn His Ser Asn Glu Asp Gly Ser Ile Gly Thr Ser Leu
1 5 10 15
His Gly Val Thr Ala Arg Glu Gln Val Phe Ser Phe Ser Val Gln Glu
20 25 30
Asp Val Pro Ser Ser Gln Ala Val Arg Thr Asn Asp Pro Thr Ala Lys
35 40 45
Phe Ala Leu Pro Val Asp Ser Glu His Arg Ala Lys Val Phe Lys Pro
50 55 60
Leu Ser Phe Ala Lys Pro His Met Arg Ala Phe His Leu Gly Trp Ile
65 70 75 80
Ser Phe Phe Thr Cys Phe Ile Ser Thr Phe Ala Ala Ala Pro Leu Val
85 90 95
Pro Val Ile Arg Asp Asn Leu Asp Leu Thr Lys Thr Asp Ile Gly Asn
100 105 110
Ala Gly Val Ala Ser Val Ser Gly Ala Ile Phe Ser Arg Leu Ala Met
115 120 125
Gly Ala Val Cys Asp Leu Leu Gly Ala Arg Tyr Gly Thr Ala Phe Ser
130 135 140
Leu Met Leu Thr Ala Pro Ala Val Phe Ser Met Ser Phe Val Ala Asp
145 150 155 160
Ala Gly Ser Tyr Leu Ala Val Arg Phe Met Ile Gly Phe Cys Leu Ala
165 170 175
Thr Phe Val Ser Cys Gln Tyr Trp Thr Ser Val Met Phe Thr Gly Lys
180 185 190
Ile Ile Gly Leu Val Asn Gly Cys Ala Gly Gly Trp Gly Asp Met Gly
195 200 205
Gly Gly Val Thr Gln Leu Leu Met Pro Met Val Phe His Val Ile Lys
210 215 220
Leu Thr Gly Ala Thr Pro Phe Thr Ala Trp Arg Phe Ala Phe Phe Ile
225 230 235 240
Pro Gly Ile Leu Gln Ile Val Met Gly Ile Leu Val Leu Thr Leu Gly
245 250 255
Gln Asp Leu Pro Asp Gly Asn Leu Ser Thr Leu Gln Lys Ser Gly Gln
260 265 270
Val Ser Lys Asp Lys Phe Ser Lys Val Phe Trp Phe Ala Val Lys Asn
275 280 285
Tyr Arg Thr Trp Ile Leu Phe Met Leu Tyr Gly Phe Ser Met Gly Val
290 295 300
Glu Leu Thr Ile Asn Asn Val Ile Ser Gly Tyr Phe Tyr Asp Arg Phe
305 310 315 320
Asn Leu Thr Leu His Thr Ala Gly Ile Ile Ala Ala Ser Phe Gly Met
325 330 335
Ala Asn Phe Phe Ala Arg Pro Phe Gly Gly Tyr Ala Ser Asp Val Ala
340 345 350
Ala Arg Leu Phe Gly Met Arg Gly Arg Leu Trp Ile Leu Trp Ile Leu
355 360 365
Gln Thr Val Gly Ala Leu Phe Cys Ile Trp Leu Gly Arg Ala Ser Ser
370 375 380
Leu Pro Ile Ala Ile Leu Ala Met Met Leu Phe Ser Met Gly Thr Gln
385 390 395 400
Ala Ala Cys Gly Ala Leu Phe Gly Val Ala Pro Phe Val Ser Arg Arg
405 410 415
Ser Leu Gly Leu Ile Ser Gly Leu Thr Gly Ala Gly Gly Asn Phe Gly
420 425 430
Ser Gly Val Thr Gln Leu Leu Phe Phe Ser Ser Ser Arg Phe Ser Thr
435 440 445
Ala Glu Gly Leu Ser Leu Met Gly Val Met Ala Val Val Cys Ser Leu
450 455 460
Pro Val Ala Phe Ile His Phe Pro Gln Trp Gly Ser Met Phe Leu Arg
465 470 475 480
Pro Ser Gln Asp Gly Glu Lys Ser Lys Glu Glu His Tyr Tyr Gly Ala
485 490 495
Glu Trp Thr Glu Glu Glu Lys Ser Leu Gly Leu His Glu Gly Ser Ile
500 505 510
Lys Phe Ala Glu Asn Ser Arg Ser Glu Arg Gly Arg Lys Ala Met Leu
515 520 525
Ala Asp Ile Pro Thr Pro Glu Thr Gly Ser Pro Ala His Val
530 535 540
<210>121
<211>1605
<212>DNA
＜213〉Radix Dauci Sativae
<400>121
atgggtgaca tggaaggttc cccgggttcc tccatgcacg gtgttaccgg tcgtgaaccg 60
tccctggctt tctccgttgc ttcccgtatc gacacctccg ctgaattcca cgttccggtt 120
gactccgaac acaaagctaa agttttcaaa ttcaaatcct tctccccgcc gcacggtctg 180
cagttccagc tgacctggat ctccttcccg acctgcttcg tttccacctt cgctgctcgt 240
ccgctggttg ttatcatccg tgacaacctg aacccgacca aaatggacgt tggtaacgct 300
ggtgttgctt ccgttaccgg ttccatcctg tcccgtggtg ctatgggtgc tatctgcgac 360
atgctgggtc cgcgttacgg ttgcgctttc ctgatcatgc tgtccgctcc gaaagttctg 420
tgcatgtcct tcgtttcctc cgctggtggt tacgttgctg ttcgtttcat gatcggtttc 480
tccctggcta ccttcgtttc ctgccgttac tggatgtcca cctccatgca ctccgacatc 540
atcggtctgg ttaacggtac cgctgctggt tggggtaaca tgaccggtgg tgctacccag 600
ctgatcatgc cgctgctgta cgacatcatc cgtgaagctg gtgctacccc gttcaccgct 660
tggcgtatcg ctctgttcat cccgggttgg ctgcacgtta tctccggtat cctggttctg 720
accctgggtc aggacctgcc ggacggtaac gctcagaacg aagcttccct gcgtaaaaaa 780
ggtcgtgttc acaaagacaa attctccaaa atcctgcgtt acgctgctac caactaccgt 840
acctggatcc tggttctgct gtacggttac tccatgggtg ttgaactgac caccgacaac 900
gttatcgctg aatacttctt cgaccgtttc gacctgaaac tgcacacccc gggtatcatc 960
gctgctacct tcggtatggc taacctgctg gctcgtccgt tcggtggttg gtcctccgac 1020
gttgctgcta aacacttcgg tatgcgtggt cgtcactgga actcctggga cctgcagacc 1080
ctgggtggtg ttttctgcct gctgctggtt cgtgctacca cctccccgat cgacgctctg 1140
gctatcacct ggatgatcat cttctccatc ggtgctcagg ctgctaccgg tgttaccttc 1200
ggtatcatcc cgttcatctc ccgtcgttac ctgggtatca tctcccagat gaccggtgct 1260
aacggtaact tcggttccgg tcagacccag ccgctggaat tcgactccac caaatacaac 1320
accggtctgg gtctgaccta catgggtatg atgatcatcg cttgcaccct gccgccgatg 1380
ctggtttggt tctggcagga cggttccatg ttcctgccgc cgtccaaaga cccgatcaaa 1440
ggtaccgaac cggaacacta cttcggttcc gaatacaccg aagacgaaaa agttaaaggt 1500
atgcaccaga actccatcaa attcgctgaa aactcccgta ccgaattcgg taaaaaacgt 1560
gttggttccg ctccgacccc gccgaacgtt taccccaacc gtgtt 1605
<210>122
<211>535
<212>PRT
＜213〉Radix Dauci Sativae
<400>122
Met Gly Asp Met Glu Gly Ser Pro Gly Ser Ser Met His Gly Val Thr
1 5 10 15
Gly Arg Glu Pro Ser Leu Ala Phe Ser Val Ala Ser Arg Ile Asp Thr
20 25 30
Ser Ala Glu Phe His Val Pro Val Asp Ser Glu His Lys Ala Lys Val
35 40 45
Phe Lys Phe Lys Ser Phe Ser Pro Pro His Gly Leu Gln Phe Gln Leu
50 55 60
Thr Trp Ile Ser Phe Pro Thr Cys Phe Val Ser Thr Phe Ala Ala Arg
65 70 75 80
Pro Leu Val Val Ile Ile Arg Asp Asn Leu Asn Pro Thr Lys Met Asp
85 90 95
Val Gly Asn Ala Gly Val Ala Ser Val Thr Gly Ser Ile Leu Ser Arg
100 105 110
Gly Ala Met Gly Ala Ile Cys Asp Met Leu Gly Pro Arg Tyr Gly Cys
115 120 125
Ala Phe Leu Ile Met Leu Ser Ala Pro Lys Val Leu Cys Met Ser Phe
130 135 140
Val Ser Ser Ala Gly Gly Tyr Val Ala Val Arg Phe Met Ile Gly Phe
145 150 155 160
Ser Leu Ala Thr Phe Val Ser Cys Arg Tyr Trp Met Ser Thr Ser Met
165 170 175
His Ser Asp Ile Ile Gly Leu Val Asn Gly Thr Ala Ala Gly Trp Gly
180 185 190
Asn Met Thr Gly Gly Ala Thr Gln Leu Ile Met Pro Leu Leu Tyr Asp
195 200 205
Ile Ile Arg Glu Ala Gly Ala Thr Pro Phe Thr Ala Trp Arg Ile Ala
210 215 220
Leu Phe Ile Pro Gly Trp Leu His Val Ile Ser Gly Ile Leu Val Leu
225 230 235 240
Thr Leu Gly Gln Asp Leu Pro Asp Gly Asn Ala Gln Asn Glu Ala Ser
245 250 255
Leu Arg Lys Lys Gly Arg Val His Lys Asp Lys Phe Ser Lys Ile Leu
260 265 270
Arg Tyr Ala Ala Thr Asn Tyr Arg Thr Trp Ile Leu Val Leu Leu Tyr
275 280 285
Gly Tyr Ser Met Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu
290 295 300
Tyr Phe Phe Asp Arg Phe Asp Leu Lys Leu His Thr Pro Gly Ile Ile
305 310 315 320
Ala Ala Thr Phe Gly Met Ala Asn Leu Leu Ala Arg Pro Phe Gly Gly
325 330 335
Trp Ser Ser Asp Val Ala Ala Lys His Phe Gly Met Arg Gly Arg His
340 345 350
Trp Asn Ser Trp Asp Leu Gln Thr Leu Gly Gly Val Phe Cys Leu Leu
355 360 365
Leu Val Arg Ala Thr Thr Ser Pro Ile Asp Ala Leu Ala Ile Thr Trp
370 375 380
Met Ile Ile Phe Ser Ile Gly Ala Gln Ala Ala Thr Gly Val Thr Phe
385 390 395 400
Gly Ile Ile Pro Phe Ile Ser Arg Arg Tyr Leu Gly Ile Ile Ser Gln
405 410 415
Met Thr Gly Ala Asn Gly Asn Phe Gly Ser Gly Gln Thr Gln Pro Leu
420 425 430
Glu Phe Asp Ser Thr Lys Tyr Asn Thr Gly Leu Gly Leu Thr Tyr Met
435 440 445
Gly Met Met Ile Ile Ala Cys Thr Leu Pro Pro Met Leu Val Trp Phe
450 455 460
Trp Gln Asp Gly Ser Met Phe Leu Pro Pro Ser Lys Asp Pro Ile Lys
465 470 475 480
Gly Thr Glu Pro Glu His Tyr Phe Gly Ser Glu Tyr Thr Glu Asp Glu
485 490 495
Lys Val Lys Gly Met His Gln Asn Ser Ile Lys Phe Ala Glu Asn Ser
500 505 510
Arg Thr Glu Phe Gly Lys Lys Arg Val Gly Ser Ala Pro Thr Pro Pro
515 520 525
Asn Val Tyr Pro Asn Arg Val
530 535
<210>123
<211>1200
<212>DNA
＜213〉Zea mays
<400>123
cagcaagatc atcggcaccg tcaacgggct cgccgccgga tggggcaaca tgggaggcgg 60
cgccacgcag ctcatcatgc cgctcgtcta cgacgtcatc cgcaagtgcg gcgccacgcc 120
attcacggcc tggcgcctcg cctacttcgt gccgggcctc atgcacgtcg tcatgggcgt 180
cctggtgctc acgctggggc aggacctccc cgacggcaac ctcaggtcgc tgcagaagaa 240
gggcaacgtc aacaaggaca gcttctccaa ggtcatgtgg tacgccgtca tcaactaccg 300
tacctggatc ttcgtcctcc tctacggcta ctgcatgggc gtcgagctca ccaccgacaa 360
cgtcatcgcc gagtacatgt acgaccgctt cgacctcgac ctccgcgtcg ccgggaccat 420
cgccgcctgc ttcggcatgg ccaacatcgt cgcacgccct atgggcggca tcatgtcgga 480
catgggcgcg cgctactggg gcatgcgcgc tcgcctctgg aacatctgga tcctccagac 540
cgccggcggc gccttctgcc tctggctggg acgcgccagc accctccccg tctccgtcgt 600
cgccatggtg ctcttctcct tctgcgcgca ggcggcctgc ggcgccatct tcggggtcat 660
ccccttcgtc tcccgccgct ccctcggcat catctccggc atgacgggcg ccggcggcaa 720
cttcggcgcc gggctcacgc agctgctctt ctttacctcc tcgacctact ccacgggcag 780
ggggctggag tacatgggca tcatgatcat ggcgtgcacg ctgccggtgg tgttcgtgca 840
cttcccgcag tgggggtcca tgttcttccc gcccagcgcc accgccgacg aggagggcta 900
ctacgcctcc gagtggaacg acgacgagaa gagcaaggga ctccatagcg ctagcctcaa 960
gttcgccgag aacagccgct cagagcgcgg caagcgcaac gtcatccagg ccgacgccgc 1020
cgccacgccg gagcatgtct aagttcacta ctaagatgga tcgatcgacg atcacctata 1080
cctctttgta tgtacgaata tgccttgtta ttactgtgcg cgcgcatata caatacacgt 1140
gtgctccgtt gacatgagtt atatagcact aaaaacttct tttgaaaaaa aaaaaaaaaa 1200
<210>124
<211>346
<212>PRT
＜213〉Zea mays
<400>124
Ser Lys Ile Ile Gly Thr Val Asn Gly Leu Ala Ala Gly Trp Gly Asn
1 5 10 15
Met Gly Gly Gly Ala Thr Gln Leu Ile Met Pro Leu Val Tyr Asp Val
20 25 30
Ile Arg Lys Cys Gly Ala Thr Pro Phe Thr Ala Trp Arg Leu Ala Tyr
35 40 45
Phe Val Pro Gly Leu Met His Val Val Met Gly Val Leu Val Leu Thr
50 55 60
Leu Gly Gln Asp Leu Pro Asp Gly Asn Leu Arg Ser Leu Gln Lys Lys
65 70 75 80
Gly Asn Val Asn Lys Asp Ser Phe Ser Lys Val Met Trp Tyr Ala Val
85 90 95
Ile Asn Tyr Arg Thr Trp Ile Phe Val Leu Leu Tyr Gly Tyr Cys Met
100 105 110
Gly Val Glu Leu Thr Thr Asp Asn Val Ile Ala Glu Tyr Met Tyr Asp
115 120 125
Arg Phe Asp Leu Asp Leu Arg Val Ala Gly Thr Ile Ala Ala Cys Phe
130 135 140
Gly Met Ala Asn Ile Val Ala Arg Pro Met Gly Gly Ile Met Ser Asp
145 150 155 160
Met Gly Ala Arg Tyr Trp Gly Met Arg Ala Arg Leu Trp Asn Ile Trp
165 170 175
Ile Leu Gln Thr Ala Gly Gly Ala Phe Cys Leu Trp Leu Gly Arg Ala
180 185 190
Ser Thr Leu Pro Val Ser Val Val Ala Met Val Leu Phe Ser Phe Cys
195 200 205
Ala Gln Ala Ala Cys Gly Ala Ile Phe Gly Val Ile Pro Phe Val Ser
210 215 220
Arg Arg Ser Leu Gly Ile Ile Ser Gly Met Thr Gly Ala Gly Gly Asn
225 230 235 240
Phe Gly Ala Gly Leu Thr Gln Leu Leu Phe Phe Thr Ser Ser Thr Tyr
245 250 255
Ser Thr Gly Arg Gly Leu Glu Tyr Met Gly Ile Met Ile Met Ala Cys
260 265 270
Thr Leu Pro Val Val Phe Val His Phe Pro Gln Trp Gly Ser Met Phe
275 280 285
Phe Pro Pro Ser Ala Thr Ala Asp Glu Glu Gly Tyr Tyr Ala Ser Glu
290 295 300
Trp Asn Asp Asp Glu Lys Ser Lys Gly Leu His Ser Ala Ser Leu Lys
305 310 315 320
Phe Ala Glu Asn Ser Arg Ser Glu Arg Gly Lys Arg Asn Val Ile Gln
325 330 335
Ala Asp Ala Ala Ala Thr Pro Glu His Val
340 345
<210>125
<211>1965
<212>DNA
＜213〉rice
<400>125
ggtggaggag agtgaggacg gcccacgcgg acgagcagag agtggcgggc tcagtgtcgg 60
gctcgccgga gtccgcgcaa tccggccgtt catgcttcat tctctcggac attaacctgg 120
tttacttgta agaaagtaga gccatggact cctcatacca gcatgacaag cctctgctgg 180
atgaagagaa ctcctcgcaa gtgacccttg aatatacagg tgatggatct gtttgcatcc 240
gtgggcatcc tgctttaagg aaacatacag ggaactggaa gggttcctca ttagccatcg 300
ttttttcatt ctgctcttat ctggccttta cttcaattgt aaaaaaccta gtcagttatc 360
tcacaaaagt tctacatgaa acaaacgtgg ccgctgcaag agatgttgca acttggtcag 420
gaacaagtta tcttgcacct ctggttggag ccttcttagc tgattcatat ctggggaagt 480
actgtacaat tctgatcttc tgcacgatct tcattatcgg attgatgatg ttgcttctgt 540
cagcagcagt tccattaatc tctactggtc ctcactcatg gatcatatgg acagatccag 600
tctcttctca gaacatcata ttctttgtcg gtttgtacat ggttgcttta gggtatggtg 660
cacagtgccc ctgcatatca tcatttggtg ctgatcaatt tgatgacact gatgaaaatg 720
agagaacaaa aaagagttct tttttcaatt ggacctattt cgtagccaat gcgggctcat 780
tgatctcggg gactgttatt gtgtgggtgc aagatcacaa aggttggatc tggggtttta 840
ccatttctgc actatttgtg tatttaggtt ttggtacttt tatctttggc tcctctatgt 900
atgatttcag aaacctggag gaagcccctc ttgcgagaat atgccaggtt gttgttgctg 960
ctattcacaa acgcgataaa gatttgccat gtgattcctc agttctttat gagtttctgg 1020
ggcagagttc agcaatcgaa ggcagccgaa aattggagca tacaactgga cttaagttct 1080
ttgatagagc tgcaatggtg acaccatctg attttgaatc tgatggccta ctaaacacat 1140
ggaagatttg cacagtcact caagtggagg aactgaagat tttgatcagg atgttccccg 1200
tttgggcaac gatgatatta tttgctgcag ttctggacaa catgttttcg acattcatag 1260
aacaggggat ggtgatggag aaacacatcg gctctttcga aatacctgcg gcgtccttcc 1320
aatccattga tgtcattgct gtccttatac tagttccagt ctatgaaaga gtccttgttc 1380
cagtgttcag aaaattcact ggcagagcaa atggcattac tccactgcag cgaatgggga 1440
tcggcctgtt cttttccatg ctctccatgg tatcagcagc attggtggag agtaatcggt 1500
tgcggattgc gcaggatgaa ggtttggtgc acaggaaggt ggctgttcca atgagcatcc 1560
tgtggcaggg accccagtac ttcctgatag gcgtgggaga ggtgttctca aacattgggt 1620
taactgaatt tttctaccag gaatcaccgg acgccatgag aagcttatgt ctcgcattct 1680
cacttgctaa cgtttcggca ggaagttacc tcagctcgtt tatcgtttct cttgtgccag 1740
tgttcacagc cagagaaggc agtcctggat ggatacctga taacttgaac gaagggcatt 1800
tggatcggtt cttctggatg atggctggct tgtgtttctt gaatatgctg gcctttgtgt 1860
tctgtgccat gaggtacaaa tgtaagaagg cttcctgaac cttgttaaca ttagcaatat 1920
aatggtggtg gaaaaggaca attgtgttgc aaaaaaaaaa aaaaa 1965
<210>126
<211>584
<212>PRT
＜213〉rice
<400>126
Met Asp Ser Ser Tyr Gln His Asp Lys Pro Leu Leu Asp Glu Glu Asn
1 5 10 15
Ser Ser Gln Val Thr Leu Glu Tyr Thr Gly Asp Gly Ser Val Cys Ile
20 25 30
Arg Gly His Pro Ala Leu Arg Lys His Thr Gly Asn Trp Lys Gly Ser
35 40 45
Ser Leu Ala Ile Val Phe Ser Phe Cys Ser Tyr Leu Ala Phe Thr Ser
50 55 60
Ile Val Lys Asn Leu Val Ser Tyr Leu Thr Lys Val Leu His Glu Thr
65 70 75 80
Asn Val Ala Ala Ala Arg Asp Val Ala Thr Trp Ser Gly Thr Ser Tyr
85 90 95
Leu Ala Pro Leu Val Gly Ala Phe Leu Ala Asp Ser Tyr Leu Gly Lys
100 105 110
Tyr Cys Thr Ile Leu Ile Phe Cys Thr Ile Phe Ile Ile Gly Leu Met
115 120 125
Met Leu Leu Leu Ser Ala Ala Val Pro Leu Ile Ser Thr Gly Pro His
130 135 140
Ser Trp Ile Ile Trp Thr Asp Pro Val Ser Ser Gln Asn Ile Ile Phe
145 150 155 160
Phe Val Gly Leu Tyr Met Val Ala Leu Gly Tyr Gly Ala Gln Cys Pro
165 170 175
Cys Ile Ser Ser Phe Gly Ala Asp Gln Phe Asp Asp Thr Asp Glu Asn
180 185 190
Glu Arg Thr Lys Lys Ser Ser Phe Phe Asn Trp Thr Tyr Phe Val Ala
195 200 205
Asn Ala Gly Ser Leu Ile Ser Gly Thr Val Ile Val Trp Val Gln Asp
210 215 220
His Lys Gly Trp Ile Trp Gly Phe Thr Ile Ser Ala Leu Phe Val Tyr
225 230 235 240
Leu Gly Phe Gly Thr Phe Ile Phe Gly Ser Ser Met Tyr Asp Phe Arg
245 250 255
Asn Leu Glu Glu Ala Pro Leu Ala Arg Ile Cys Gln Val Val Val Ala
260 265 270
Ala Ile His Lys Arg Asp Lys Asp Leu Pro Cys Asp Ser Ser Val Leu
275 280 285
Tyr Glu Phe Leu Gly Gln Ser Ser Ala Ile Glu Gly Ser Arg Lys Leu
290 295 300
Glu His Thr Thr Gly Leu Lys Phe Phe Asp Arg Ala Ala Met Val Thr
305 310 315 320
Pro Ser Asp Phe Glu Ser Asp Gly Leu Leu Asn Thr Trp Lys Ile Cys
325 330 335
Thr Val Thr Gln Val Glu Glu Leu Lys Ile Leu Ile Arg Met Phe Pro
340 345 350
Val Trp Ala Thr Met Ile Leu Phe Ala Ala Val Leu Asp Asn Met Phe
355 360 365
Ser Thr Phe Ile Glu Gln Gly Met Val Met Glu Lys His Ile Gly Ser
370 375 380
Phe Glu Ile Pro Ala Ala Ser Phe Gln Ser Ile Asp Val Ile Ala Val
385 390 395 400
Leu Ile Leu Val Pro Val Tyr Glu Arg Val Leu Val Pro Val Phe Arg
405 410 415
Lys Phe Thr Gly Arg Ala Asn Gly Ile Thr Pro Leu Gln Arg Met Gly
420 425 430
Ile Gly Leu Phe Phe Ser Met Leu Ser Met Val Ser Ala Ala Leu Val
435 440 445
Glu Ser Asn Arg Leu Arg Ile Ala Gln Asp Glu Gly Leu Val His Arg
450 455 460
Lys Val Ala Val Pro Met Ser Ile Leu Trp Gln Gly Pro Gln Tyr Phe
465 470 475 480
Leu Ile Gly Val Gly Glu Val Phe Ser Asn Ile Gly Leu Thr Glu Phe
485 490 495
Phe Tyr Gln Glu Ser Pro Asp Ala Met Arg Ser Leu Cys Leu Ala Phe
500 505 510
Ser Leu Ala Asn Val Ser Ala Gly Ser Tyr Leu Ser Ser Phe Ile Val
515 520 525
Ser Leu Val Pro Val Phe Thr Ala Arg Glu Gly Ser Pro Gly Trp Ile
530 535 540
Pro Asp Asn Leu Asn Glu Gly His Leu Asp Arg Phe Phe Trp Met Met
545 550 555 560
Ala Gly Leu Cys Phe Leu Asn Met Leu Ala Phe Val Phe Cys Ala Met
565 570 575
Arg Tyr Lys Cys Lys Lys Ala Ser
580
<210>127
<211>405
<212>DNA
＜213〉Arabidopis thaliana
<400>127
tcatactcac agaacagaaa ccctaatttc ctcaaagcaa tctcgtttca tcttccgcaa 60
acgaaaccct cgagagttgt tactcctcct cctcctctgg tgcatgataa agcggcctca 120
gggtttgctg cggcgctcgt tagcgtttgt caatcgaaga attgtcttgg tcggactcaa 180
gaagatgtca gaagattgat ggagtttctt gtgggagaag aaaagaagag gaacaaggtc 240
ttagtcaatg atgttgttga gagaggtaaa tttggtaaac acttcaaggg cttggtcaag 300
atgttgattg caagaggtaa atctgggatt ctggtggatg ttttaatgga atttgagaga 360
atctgtaatg aattggtctc aaaaaaactt gtctgggttt cttga 405
<210>128
<211>135
<212>PRT
＜213〉Arabidopis thaliana
<400>128
Ala Ser Tyr Ser Gln Asn Arg Asn Pro Asn Phe Leu Lys Ala Ile Ser
1 5 10 15
Phe His Leu Pro Gln Thr Lys Pro Ser Arg Val Val Thr Pro Pro Pro
20 25 30
Pro Leu Val His Asp Lys Ala Ala Ser Gly Phe Ala Ala Ala Leu Val
35 40 45
Ser Val Cys Gln Ser Lys Asn Cys Leu Gly Arg Thr Gln Glu Asp Val
50 55 60
Arg Arg Leu Met Glu Phe Leu Val Gly Glu Glu Lys Lys Arg Asn Lys
65 70 75 80
Val Leu Val Asn Asp Val Val Glu Arg Gly Lys Phe Gly Lys His Phe
85 90 95
Lys Gly Leu Val Lys Met Leu Ile Ala Arg Gly Lys Ser Gly Ile Leu
100 105 110
Val Asp Val Leu Met Glu Phe Glu Arg Ile Cys Asn Glu Leu Val Ser
115 120 125
Lys Lys Leu Val Trp Val Ser
130 135
<210>129
<211>576
<212>DNA
＜213〉Arabidopis thaliana
<400>129
atggatactc tctcagcatc cgtttcatcc ttgaacctcc cttctcttcc tccaccaccg 60
cagccaccgt tgagatccat ctccagccgc tttaaatcca ccgtaaacgc caccacttct 120
gcttcatcta ccaatctttc aaaaccaaca tcgtcttcac cttcttcagc atcatactca 180
cagaacagaa accctaattt cctcaaagca atctcgtttc atcttccgca aacgaaaccc 240
tcgagagttg ttactcctcc tcctcctctg gtgcatgata aagcggcctc agggtttgct 300
gcggcgctcg ttagcgtttg tcaatcgaag aattgtcttg gtcggactca agaagatgtc 360
agaagattga tggagtttct tgtgggagaa gaaaagaaga ggaacaaggt cttagtcaat 420
gatgttgttg agagaggtaa atttggtaaa cacttcaagg gcttggtcaa gatgttgatt 480
gcaagaggta aatctgggat tctggtggat gttttaatgg aatttgagag aatctgtaat 540
gaattggtct caaaaaaact tgtctgggtt tcttga 576
<210>130
<211>191
<212>PRT
＜213〉Arabidopis thaliana
<400>130
Met Asp Thr Leu Ser Ala Ser Val Ser Ser Leu Asn Leu Pro Ser Leu
1 5 10 15
Pro Pro Pro Pro Gln Pro Pro Leu Arg Ser Ile Ser Ser Arg Phe Lys
20 25 30
Ser Thr Val Asn Ala Thr Thr Ser Ala Ser Ser Thr Asn Leu Ser Lys
35 40 45
Pro Thr Ser Ser Ser Pro Ser Ser Ala Ser Tyr Ser Gln Asn Arg Asn
50 55 60
Pro Asn Phe Leu Lys Ala Ile Ser Phe His Leu Pro Gln Thr Lys Pro
65 70 75 80
Ser Arg Val Val Thr Pro Pro Pro Pro Leu Val His Asp Lys Ala Ala
85 90 95
Ser Gly Phe Ala Ala Ala Leu Val Ser Val Cys Gln Ser Lys Asn Cys
100 105 110
Leu Gly Arg Thr Gln Glu Asp Val Arg Arg Leu Met Glu Phe Leu Val
115 120 125
Gly Glu Glu Lys Lys Arg Asn Lys Val Leu Val Asn Asp Val Val Glu
130 135 140
Arg Gly Lys Phe Gly Lys His Phe Lys Gly Leu Val Lys Met Leu Ile
145 150 155 160
Ala Arg Gly Lys Ser Gly Ile Leu Val Asp Val Leu Met Glu Phe Glu
165 170 175
Arg Ile Cys Asn Glu Leu Val Ser Lys Lys Leu Val Trp Val Ser
180 185 190
<210>131
<211>57
<212>PRT
＜213〉Arabidopis thaliana
<400>131
Met Asp Thr Leu Ser Ala Ser Val Ser Ser Leu Asn Leu Pro Ser Leu
1 5 10 15
Pro Pro Pro Pro Gln Pro Pro Leu Arg Ser Ile Ser Ser Arg Phe Lys
20 25 30
Ser Thr Val Asn Ala Thr Thr Ser Ala Ser Ser Thr Asn Leu Ser Lys
35 40 45
Pro Thr Ser Ser Ser Pro Ser Ser Ser
50 55
<210>132
<211>32
<212>PRT
＜213〉chlamydomonas (Chlamydomonas)
<400>132
Met Ala Met Ala Met Arg Ser Thr Phe Ala Ala Arg Val Gly Ala Lys
1 5 10 15
Pro Ala Val Arg Gly Ala Arg Pro Ala Ser Arg Met Ser Cys Met Ala
20 25 30
<210>133
<211>32
<212>PRT
＜213〉chlamydomonas
<400>133
Met Gln Val Thr Met Lys Ser Ser Ala Val Ser Gly Gln Arg Val Gly
1 5 10 15
Gly Ala Arg Val Ala Thr Arg Ser Val Arg Arg Ala Gln Leu Gln Val
20 25 30
<210>134
<211>52
<212>PRT
＜213〉Arabidopis thaliana
<400>134
Met Ala Ser Leu Met Leu Ser Leu Gly Ser Thr Ser Leu Leu Pro Arg
1 5 10 15
Glu Ile Asn Lys Asp Lys Leu Lys Leu Gly Thr Ser Ala Ser Asn Pro
20 25 30
Phe Leu Lys Ala Lys Ser Phe Ser Arg Val Thr Met Thr Val Ala Val
35 40 45
Lys Pro Ser Arg
50
<210>135
<211>54
<212>PRT
＜213〉Arabidopis thaliana
<400>135
Met Ala Thr Gln Phe Ser Ala Ser Val Ser Leu Gln Thr Ser Cys Leu
1 5 10 15
Ala Thr Thr Arg Ile Ser Phe Gln Lys Pro Ala Leu Ile Ser Asn His
20 25 30
Gly Lys Thr Asn Leu Ser Phe Asn Leu Arg Arg Ser Ile Pro Ser Arg
35 40 45
Arg Leu Ser Val Ser Cys
50
<210>136
<211>70
<212>PRT
＜213〉Arabidopis thaliana
<400>136
Met Ala Ser Ile Ala Ala Ser Ala Ser Ile Ser Leu Gln Ala Arg Pro
1 5 10 15
Arg Gln Leu Ala Ile Ala Ala Ser Gln Val Lys Ser Phe Ser Asn Gly
20 25 30
Arg Arg Ser Ser Leu Ser Phe Asn Leu Arg Gln Leu Pro Thr Arg Leu
35 40 45
Thr Val Ser Cys Ala Ala Lys Pro Glu Thr Val Asp Lys Val Cys Ala
50 55 60
Val Val Arg Lys Gln Leu
65 70
<210>137
<211>74
<212>PRT
＜213〉Arabidopis thaliana
<400>137
Met Ala Ser Ile Ala Thr Ser Ala Ser Thr Ser Leu Gln Ala Arg Pro
1 5 10 15
Arg Gln Leu Val Ile Gly Ala Lys Gln Val Lys Ser Phe Ser Tyr Gly
20 25 30
Ser Arg Ser Asn Leu Ser Phe Asn Leu Arg Gln Leu Pro Thr Arg Leu
35 40 45
Thr Val Tyr Cys Ala Ala Lys Pro Glu Thr Val Asp Lys Val Cys Ala
50 55 60
Val Val Arg Lys Gln Leu Ser Leu Lys Glu
65 70
<210>138
<211>51
<212>PRT
＜213〉Arabidopis thaliana
<400>138
Met Ala Ser Ser Ala Ala Ala Ile Val Ser Gly Ser Pro Phe Arg Ser
1 5 10 15
Ser Pro Leu Ile His Asn His His Ala Ser Arg Tyr Ala Pro Gly Ser
20 25 30
Ile Ser Val Val Ser Leu Pro Arg Gln Val Ser Arg Arg Gly Leu Ser
35 40 45
Val Lys Ser
50
<210>139
<211>100
<212>PRT
＜213〉colea
<400>139
Met Ala Thr Ala Asn Ala Leu Ser Ser Pro Ser Val Leu Cys Ser Ser
1 5 10 15
Arg Gln Gly Lys Leu Ser Gly Gly Ser Gln Gln Lys Gly Gln Arg Val
20 25 30
Ser Tyr Arg Lys Ala Asn Arg Arg Phe Ser Leu Arg Ala Asn Val Lys
35 40 45
Glu Ile Ala Phe Asp Gln Ser Ser Arg Ala Ala Leu Gln Ala Gly Ile
50 55 60
Asp Lys Leu Ala Asp Ala Val Gly Leu Thr Leu Gly Pro Arg Gly Arg
65 70 75 80
Asn Val Val Leu Asp Glu Phe Gly Ser Pro Lys Val Val Asn Asp Gly
85 90 95
Val Thr Ile Ala
100
<210>140
<211>100
<212>PRT
＜213〉barley
<400>140
Met Ala Pro Thr Val Met Ala Ser Ser Ala Thr Ser Val Ala Pro Phe
1 5 10 15
Gln Gly Leu Lys Ser Thr Ala Gly Leu Pro Val Ser Arg Arg Ser Asn
20 25 30
Ala Ser Ser Ala Ser Val Ser Asn Gly Gly Arg Ile Arg Cys Met Gln
35 40 45
Val Trp Pro Ile Glu Gly Ile Lys Lys Phe Glu Thr Leu Ser Tyr Leu
50 55 60
Pro Pro Leu Ser Thr Glu Ala Leu Leu Lys Gln Val Asp Tyr Leu Ile
65 70 75 80
Arg Ser Lys Trp Val Pro Cys Leu Glu Phe Ser Lys Val Gly Phe Ile
85 90 95
Phe Arg Glu His
100
<210>141
<211>100
<212>PRT
＜213〉puncture vine clover
<400>141
Met Thr Thr Ile Phe Arg Leu Ala Ser Ser Ser Ser Pro Ser Leu Arg
1 5 10 15
His Asp Ala Thr Pro His Asn Phe His Ile Arg Lys Thr Ser Ile Ser
20 25 30
Asn Thr Phe Ser Phe Ser Ser Lys Asn Ser Leu Ser Phe Lys Arg Ile
35 40 45
Leu Thr Ser Gly Gly Ser Arg Arg Phe Ile Val Ala Ala Ser Pro Pro
50 55 60
Thr Glu Asp Ala Val Val Ala Thr Glu Pro Leu Thr Lys Gln Asp Leu
65 70 75 80
Ile Asp Tyr Leu Ala Ser Gly Cys Lys Thr Lys Asp Lys Trp Arg Ile
85 90 95
Gly Thr Glu His
100
<210>142
<211>100
<212>PRT
＜213〉spinach (Spinacia oleracea)
<400>142
Met Glu Thr Ser Met Ala Cys Cys Ser Arg Ser Ile Val Leu Pro Arg
1 5 10 15
Val Ser Pro Gln His Ser Ser Ala Leu Val Pro Ser Ser Ile Asn Leu
20 25 30
Lys Ser Leu Lys Ser Ser Ser Leu Phe Gly Glu Ser Leu Arg Met Thr
35 40 45
Thr Lys Ser Ser Val Arg Val Asn Lys Ala Lys Asn Ser Ser Leu Val
50 55 60
Thr Lys Cys Glu Leu Gly Asp Ser Leu Glu Glu Phe Leu Ala Lys Ala
65 70 75 80
Thr Thr Asp Lys Gly Leu Ile Arg Leu Met Met Cys Met Gly Glu Ala
85 90 95
Leu Arg Thr Ile
100
<210>143
<211>1236
<212>DNA
＜213〉rice
<400>143
ggtcagccaa tacattgatc cgttgccaat catgcaaagt attttggctg tggccgagtg 60
ccggaattga taattgtgtt ctgactaaat taaatgacca gaagtcgcta tcttccaatg 120
tatccgaaac ctggattaaa caatcctgtt ctgttctcta gcccctcctg catggccgga 180
ttgttttttt gacatgtttt cttgactgag gcctgtttgt tctaaacttt ttcttcaaac 240
ttttaacttt ttcatcacat cagaactttt ctacacatat aaacttttaa cttttccgtc 300
acatcgttcc aatttcaatc aaactttcaa ttttggcgtg aactaaacac accctgagtc 360
ttttattgct cctccgtacg ggttggctgg ttgagaatag gtattttcag agagaaaatc 420
tagatattgg gaggaacttg gcatgaatgg ccactatatt tagagcaatt ctacggtcct 480
tgaggaggta ccatgaggta ccaaaatttt agtgtaaatt ttagtatctc attataacta 540
ggtattatga ggtaccaaat ttacaataga aaaaatagta cttcatggta ctttcttaag 600
taccgtaaaa ttgctcctat atttaagggg atgtttatat ctatccatat ccataatttg 660
attttgataa gaaaaaatgt gagcacacca agcatgtcca tgaccttgca ctcttggctc 720
actcgtcaac tgtgaagaac ctcaaaaatg ctcaatatag ctacaggtgc ctgaaaaaat 780
aactttaaag ttttgaacat cgatttcact aaacaacaat tattatctcc ctctgaaaga 840
tgatagttta gaactctaga atcattgtcg gcggagaaag taaattattt tccccaaatt 900
tccagctatg aaaaaaccct caccaaacac catcaaacaa gagttcacca aaccgcccat 960
gcggccatgc tgtcacgcaa cgcaccgcat tgcctgatgg ccgctcgatg catgcatgct 1020
tccccgtgca catatccgac agacgcgccg tgtcagcgag ctcctcgacc gacctgtgta 1080
gcccatgcaa gcatccaccc ccgccacgta caccccctcc tcctccctac gtgtcaccgc 1140
tctctccacc tatatatgcc cacctggccc ctctcctccc atctccactt cacccgatcg 1200
cttcttcttc ttcttcgttg cattcatctt gctagc 1236
<210>144
<211>55
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm00735
<400>144
ggggacaagt ttgtacaaaa aagcaggctt cacaatggat actctctcag catcc 55
<210>145
<211>50
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm00736
<400>145
ggggaccact ttgtacaaga aagctgggtt gtatcatcaa gaaacccaga 50
<210>146
<211>1592
<212>DNA
＜213〉rice
<400>146
caaaagcaag aggaggaggc cgcggctagc gagcgagcga gagagagggg agaagaagag 60
gtgggacagc cgggagatcc atccctgtgg agaggaggga gggaggaagg aggcgttgga 120
ggaggagagg ttgaccgata gatccattgc ggagttgagt gttgatgcaa agctgattcg 180
ccatcgttta gctttttata agagatgggt tcagtagggg ttgcgccgtc tgggttaaag 240
aacagcagta gcaccagcat gggtgctgag aagttgcctg atcagatgca tgatctgaag 300
ataagggacg ataaggaagt tgaagcgact attattaacg gcaagggaac agaaaccggc 360
cacataattg tcacaactac tggtggcaga aatggtcagc cgaaacagac agttagctac 420
atggctgagc gtattgtagg gcaaggttca tttgggattg tcttccaggc aaaatgtctg 480
gagacaggtg agacagttgc tatcaagaag gttcttcagg ataagcgcta caagaaccgt 540
gagcttcaga ccatgcgcct tcttgaccac ccaaatgttg tagctctgaa gcactgtttc 600
ttctctacaa ctgagaagga tgaactgtat ctaaacttgg ttcttgagta tgtgcctgaa 660
actgttcatc gtgttgtgaa gcattacaac aagatgaacc agcgtatgcc acttatctat 720
gtgaagctgt atatgtacca gatttgtagg gcattagctt acatccataa tagcatcgga 780
gtttgccaca gagatatcaa gccacagaat cttctggtaa acccacatac ccatcaactc 840
aagctatgtg actttgggag tgcaaaagtt ctggtcaagg gagaaccgaa catatcgtac 900
atttgctccc gatactatag ggctccggag ctcatatttg gtgccaccga atacactaca 960
gctattgaca tctggtctgc tggatgtgtt cttgctgaac ttatgttagg gcagcctctg 1020
tttcctggtg aaagtggtgt agaccaactt gtggaaatca tcaaggtcct tggaacacct 1080
acaagggagg aaattaaatg catgaatcca aactataccg agttcaagtt tccacagatt 1140
aaagcacacc catggcacaa ggtattccat aaaaggttgc ctccagaagc tgttgatctt 1200
gtctctaggc tgctccagta ctcacccaac ctaagatgca ctgctgtgga agcacttgtt 1260
cacccattct ttgatgagct tcgagaccct aatgctcgcc ttccgaatgg ccgctttttg 1320
cctcctctct tcaacttcaa gcctcatgaa ctgaaaggaa tcccatcaga tattatggcg 1380
aaattgatcc cagaacatgt gaagaagcaa tgctcctatg caggagtatg agacagcttc 1440
cgcacgaccc ccctggaaat ttccatgaca agtgcccatt tcctcccccc tggacgacga 1500
tggatcgtca gcatatgcgt gcatgatggt tggtgaggat gtgaagttac gttgttgttg 1560
tgtgaccacc tagagcttga acagaaggaa aa 1592
<210>147
<211>408
<212>PRT
＜213〉rice
<400>147
Met Gly Ser Val Gly Val Ala Pro Ser Gly Leu Lys Asn Ser Ser Ser
1 5 10 15
Thr Ser Met Gly Ala Glu Lys Leu Pro Asp Gln Met His Asp Leu Lys
20 25 30
Ile Arg Asp Asp Lys Glu Val Glu Ala Thr Ile Ile Asn Gly Lys Gly
35 40 45
Thr Glu Thr Gly His Ile Ile Val Thr Thr Thr Gly Gly Arg Asn Gly
50 55 60
Gln Pro Lys Gln Thr Val Ser Tyr Met Ala Glu Arg Ile Val Gly Gln
65 70 75 80
Gly Ser Phe Gly Ile Val Phe Gln Ala Lys Cys Leu Glu Thr Gly Glu
85 90 95
Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys Asn Arg
100 105 110
Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ala Leu
115 120 125
Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu Asn
130 135 140
Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Val Lys His
145 150 155 160
Tyr Asn Lys Met Asn Gln Arg Met Pro Leu Ile Tyr Val Lys Leu Tyr
165 170 175
Met Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile Hi s Asn Ser Ile Gly
180 185 190
Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro His
195 200 205
Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu Val
210 215 220
Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala
225 230 235 240
Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp Ile
245 250 255
Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Met Leu Gly Gln Pro Leu
260 265 270
Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val
275 280 285
Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn Tyr
290 295 300
Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys Val
305 310 315 320
Phe His Lys Arg Leu Pro Pro Glu Ala Val Asp Leu Val Ser Arg Leu
325 330 335
Leu Gln Tyr Ser Pro Asn Leu Arg Cys Thr Ala Val Glu Ala Leu Val
340 345 350
His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro Asn
355 360 365
Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu Leu Lys
370 375 380
Gly Ile Pro Ser Asp Ile Met Ala Lys Leu Ile Pro Glu His Val Lys
385 390 395 400
Lys Gln Cys Ser Tyr Ala Gly Val
405
<210>148
<211>1800
<212>DNA
＜213〉rice
<400>148
ctctccgaat cctcccccgc attccgcgcc ggagctgagg agggacagcg agccagcgag 60
ggaggtgggg caatccagcg agcgccagat cgtgccgcca gccccgctcc gtcctctctc 120
cggcgaccct ccgccccgcc tgatcccgtg gtggatcaac tacaactcat ctttaggaaa 180
gcctcttgaa cattagcctt gtgtgccttt caacagtatg gcctcggtag gtgtggtgcg 240
ttcttccttg gggtttcaga acgaaacaag tacaagtggc gatgccgacc gacttccgaa 300
tgagatgagc aatatgagta taagggatga taataaggac attgacgaca tagttgtcaa 360
tggcaatggg acagaacctg gtcatgttat tgtgacaagc attgatggaa gaaatggaca 420
ggctaaacag accattagct acatggccga gcgtgtcgta ggtcatggat cctttggaac 480
tgttttccag gccaagtgcc tggaaaccgg tgagactgtg gctataaaga aggttcttca 540
agacaagaga tataagaacc gtgagctgca aacaatgcga gttcttgacc acccaaatgt 600
ggtgtcacta aagcattgtt ttttctcaaa gactgagaag gaggagcttt acctcaattt 660
ggtgcttgag tatgtgccag agactgctca ccgtgtaatt aaacattaca acaagatgaa 720
ccaacgcatg ccattgatat atgcaaaact atacatgtat cagatatgca gagctttggc 780
gtacattcac aacaccattg gcgtgtgtca cagggatatc aagccacaaa atcttctggt 840
gaacccacat actcatcagc tgaaattatg tgacttcggc agcgcgaaag tcttggtaaa 900
aggagaacca aatatttctt acatctgttc aaggtactac agagctccag agctcatatt 960
tggtgctact gaatacacaa cagcgattga tgtttggtct gccggttgtg tgcttgctga 1020
actcctcctg gggcagcctc tattccctgg cgacagtggc gttgatcagc ttgttgaaat 1080
catcaaggtt ctgggtaccc ctacacgaga ggagattaag tgcatgaacc caaattacac 1140
cgagtttaaa tttccgcaaa tcaaagctca cccatggcac aagatcttcc ataaaagaat 1200
gcctgctgaa gcagtcgatc tcgtctccag gcttttgcag tattcaccat acctccggtc 1260
cactgcttcg gaagcattga tccatccctt cttcgatgaa ctccgtgatc caaacacccg 1320
cttaccgaac ggccgtttcc ttcctcctct cttcaacttc aagccccatg aactgaaggg 1380
tatgccaatg gaattcctgg tgaagcttat ccccgaacat gctcgaaagc aatgcgcgtt 1440
tgtaggatgg tgatttctga ggtcagcatg aaaactagtt cagaatttct tcaccgtcct 1500
ccattagaaa gcagagatga accctgtgtg cagccatttg ggaaagctgg tgcatatgga 1560
agtggaacta catttttttg tccgagattc tgacgccgcg tattcttttc ccccctccca 1620
ctttgctgct gccggtgtaa ccaaaaaatc atccacggtt ctgtaaagtt gatgaagaag 1680
agtgtaaaat caggttgaaa actgaattcg atcggtttgt caagattgta gcaacatgca 1740
aggaaggatg ttgcacactt tgtatggcaa tgttcgttcg gtccaaatat ttggacatgg 1800
<210>149
<211>411
<212>PRT
＜213〉rice
<400>149
Met Ala Ser Val Gly Val Val Arg Ser Ser Leu Gly Phe Gln Asn Glu
1 5 10 15
Thr Ser Thr Ser Gly Asp Ala Asp Arg Leu Pro Asn Glu Met Ser Asn
20 25 30
Met Ser Ile Arg Asp Asp Asn Lys Asp Ile Asp Asp Ile Val Val Asn
35 40 45
Gly Asn Gly Thr Glu Pro Gly His Val Ile Val Thr Ser Ile Asp Gly
50 55 60
Arg Asn Gly Gln Ala Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val
65 70 75 80
Val Gly Hi s Gly Ser Phe Gly Thr Val Phe Gln Ala Lys Cys Leu Glu
85 90 95
Thr Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr
100 105 110
Lys Asn Arg Glu Leu Gln Thr Met Arg Val Leu Asp His Pro Asn Val
115 120 125
Val Ser Leu Lys His Cys Phe Phe Ser Lys Thr Glu Lys Glu Glu Leu
130 135 140
Tyr Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Ala His Arg Val
145 150 155 160
Ile Lys His Tyr Asn Lys Met Asn Gln Arg Met Pro Leu Ile Tyr Ala
165 170 175
Lys Leu Tyr Met Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Asn
180 185 190
Thr Ile Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val
195 200 205
Asn Pro His Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys
210 215 220
Val Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr
225 230 235 240
Tyr Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala
245 250 255
Ile Asp Val Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly
260 265 270
Gln Pro Leu Phe Pro Gly Asp Ser Gly Val Asp Gln Leu Val Glu Ile
275 280 285
Ile Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn
290 295 300
Pro Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp
305 310 315 320
His Lys Ile Phe His Lys Arg Met Pro Ala Glu Ala Val Asp Leu Val
325 330 335
Ser Arg Leu Leu Gln Tyr Ser Pro Tyr Leu Arg Ser Thr Ala Ser Glu
340 345 350
Ala Leu Ile His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg
355 360 365
Leu Pro Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His
370 375 380
Glu Leu Lys Gly Met Pro Met Glu Phe Leu Val Lys Leu Ile Pro Glu
385 390 395 400
His Ala Arg Lys Gln Cys Ala Phe Val Gly Trp
405 410
<210>150
<211>2261
<212>DNA
＜213〉rice
<400>150
ttggggcgga gagacgggag agagaggaga ggagtcgaac tagggttagt gaggtgaagg 60
ggggcgattt tgctcatcgc cctccgccta gatcgaatcc gaatcccgcc tcctcctcct 120
cctcctcctc cccccgcgcc gatcttgccc cgctccctcg ccgcagctgc gggcttgccc 180
tagcccgccg catcgccgac gtactacgcc cgctcggctc caggctccag atccggcctc 240
cagggcgtgc ccgctgctgg acggcgctct cccgcgtccg tggctacctc ctcctccggg 300
tttgcgttgc cgtcgccgaa gcgatccgtt ggtggattgt gtgtagctct gtaacaaagg 360
atcttggtcc ataggagtga accagaaggt acaaaacttc ttggctatta tgacatcggt 420
gggtctagta gattcttcat caggtttccc ggaaacgagt actagtggag ccactgatcg 480
tctgacagat gatatcagcg agatgagcat aagagataag gaagttgaag ctgtagtggt 540
cagtggcaat agtatggaca taggtcacac tattgtgaca actgttggtg gaagaaatgg 600
gcagccaaag cagacaatta gttacatcgc agagcgtgct gttgggcgtg gttcatttgg 660
agttgtcttc caggctaagt gtctagaaac tggtgagaga gttgctgtaa agaaagttct 720
tcaagatgcg agatacaaga accgggagct gcaaacgatg caagttctcg atcacccaaa 780
tgttgcatgc ttgaagcatt acttttgctc gactactgcg aaggaggagc tatacctcaa 840
cttggttctt gaatatgtgc cagaaactgt tcatcgtgtt attagacatt acaataagat 900
gagccaacgc atgccattga tttatgttaa actttatatg taccagattt gtagggcttt 960
ggcatacatt cataactgtg tgggagtatg ccacagagat ataaagccac agaatatatt 1020
ggtgaatcca cataatcatc agttgaaatt gtgtgacttt ggcagtgcaa aagtcctggt 1080
gaaaggggaa ccaaatattt catatatctg ttctagatac tacagagccc cagcactcat 1140
atttggggct acagagtaca cgacagctat tgatgtgtgg tctgctggct gtgttcttgc 1200
tgaactactt ctagggcagc ctgtttttcc tggtgacagt ggcgtcgacc aacttgttga 1260
aattatcaag gtactgggta ctccaacaag ggaagaaatc aaacatatga atccgaacta 1320
cactgagttt aagttccccc aaatcaaagc tcacccatgg cacaagatct ttcataaaag 1380
aatgccatct gaagctgtag atcttgtgtc tcggcttctg cagtactcac cacacctccg 1440
gtgcagtgca ttggaggtgt taattcatcc attcttcgat gaactacgag atccaaatgc 1500
tcgcttacca aatggccgta ctcttccccc actattcaat ttcaagcctc gtgagctgaa 1560
aggagcatca atggaatttc ttgtgaagtt ggttccacag catgctaaga agcaatgtgc 1620
cttcttagga ttatgagcag acagttcata cactaatcgg gtattacgtt ggtctacttg 1680
gttccgtaga acagcatggc ctgcagtatt attgaatggg ccctaatttt gttctacgtg 1740
caccctgctt gggcatgcct tctaccatca tattctgtaa agcagatgaa gacatggagt 1800
gagtggttat tgattggggc ttgtatattg gtctgccctt gacttgagaa ctttattcac 1860
tctgctgtat caagcaagaa agaacacgta tgtatgcttg agccatattt cattccagag 1920
cctctatcag gtttcaccga ccatcagatt taagtcttcc agtttgtagg gctaagccaa 1980
aaaagttcta tgtgtgaaga gttggagaca tctgtttggt tacaagctat ttgattgtat 2040
ttgatcattc ttttgtatta acactctgat aaacttacat gagttaattc tattgttatg 2100
tgatatagtt gtgtgccact tttaaacacc taaacaactc tgttcaatgt tgaaatgttt 2160
ggcatgtatc aacatatcac ctcattattt cgaggttgat tttgtacacc tgatatctgt 2220
tagcttgaat gcttgattac aagcaaatga ttaaatattt t 2261
<210>151
<211>408
<212>PRT
＜213〉rice
<400>151
Met Thr Ser Val Gly Leu Val Asp Ser Ser Ser Gly Phe Pro Glu Thr
1 5 10 15
Ser Thr Ser Gly Ala Thr Asp Arg Leu Thr Asp Asp Ile Ser Glu Met
20 25 30
Ser Ile Arg Asp Lys Glu Val Glu Ala Val Val Val Ser Gly Asn Ser
35 40 45
Met Asp Ile Gly His Thr Ile Val Thr Thr Val Gly Gly Arg Asn Gly
50 55 60
Gln Pro Lys Gln Thr Ile Ser Tyr Ile Ala Glu Arg Ala Val Gly Arg
65 70 75 80
Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly Glu
85 90 95
Arg Val Ala Val Lys Lys Val Leu Gln Asp Ala Arg Tyr Lys Asn Arg
100 105 110
Glu Leu Gln Thr Met Gln Val Leu Asp His Pro Asn Val Ala Cys Leu
115 120 125
Lys His Tyr Phe Cys Ser Thr Thr Ala Lys Glu Glu Leu Tyr Leu Asn
130 135 140
Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Arg His
145 150 155 160
Tyr Asn Lys Met Ser Gln Arg Met Pro Leu Ile Tyr Val Lys Leu Tyr
165 170 175
Met Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Asn Cys Val Gly
180 185 190
Val Cys His Arg Asp Ile Lys Pro Gln Asn Ile Leu Val Asn Pro His
195 200 205
Asn His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu Val
210 215 220
Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala
225 230 235 240
Pro Ala Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr AlaIle Asp Val
245 250 255
Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Val
260 265 270
Phe Pro Gly Asp Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val
275 280 285
Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys His Met Asn Pro Asn Tyr
290 295 300
Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys Ile
305 310 315 320
Phe His Lys Arg Met Pro Ser Glu Ala Val Asp Leu Val Ser Arg Leu
325 330 335
Leu Gln Tyr Ser Pro His Leu Arg Cys Ser Ala Leu Glu Val Leu Ile
340 345 350
His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro Asn
355 360 365
Gly Arg Thr Leu Pro Pro Leu Phe Asn Phe Lys Pro Arg Glu Leu Lys
370 375 380
Gly Ala Ser Met Glu Phe Leu Val Lys Leu Val Pro Gln His Ala Lys
385 390 395 400
Lys Gln Cys Ala Phe Leu Gly Leu
405
<210>152
<211>1634
<212>DNA
＜213〉Arabidopis thaliana
<400>152
acaaaataat ataaagtaaa aatatttcat aattcgtctc tcaccggaaa aataaataaa 60
gttagagaga gagattcata atcatcaaac ccacaaatcc tttttttttt tttttgttaa 120
aacccacaaa tccttactcg tacggatctc tctctcttta atatcctaat tcttctcttc 180
atcgatctga gatttttacg ttttcatcgg cttgaaagtt tgaagagttt tgtagcctga 240
aaaatggcgt cagtgggtat agctcctaat cctggagcaa gagactctac tggtgttgat 300
aaattgcctg aagaaatgaa tgacatgaaa attcgtgacg ataaagaaat ggaagcgaca 360
gtggtagatg gaaatggaac agagactgga catatcattg tgactactat tggtggtaga 420
aatggccaac caaaacagac aattagctac atggctgagc gtgttgttgg tcacggatct 480
tttggtgttg tgttccaagc gaaatgtctt gagacaggag aaactgttgc gataaagaaa 540
gttttacaag ataggaggta caagaaccgt gagcttcaaa ccatgaggct acttgaccat 600
cctaatgttg tgtctctgaa acattgtttc ttctcaacca ctgaaaaaga tgagctttac 660
ctcaatcttg ttcttgagta cgttccagaa actgttcatc gtgttatcaa acactacaac 720
aaactgaatc agagaatgcc tcttatatac gtcaaacttt acacttatca gatttttaga 780
gccttatctt acattcaccg atgcattggt gtgtgtcatc gtgacataaa acctcaaaac 840
ttgttggtaa atccgcacac tcatcaagta aagctatgtg attttggaag tgcaaaagta 900
ttggtaaaag gagaaccaaa catttcctac atctgctcga ggtattacag agcacctgaa 960
cttatttttg gagcaaccga gtatacgaca gccattgatg tctggtctgc aggatgtgtt 1020
ctagctgaac tattgcttgg acagcccttg ttccctggtg agagcggtgt tgatcaactt 1080
gtagagatta tcaaggtctt gggaacgcct actagagaag aaatcaagtg catgaaccca 1140
aactacacgg aattcaaatt ccctcagatt aaagctcatc catggcacaa gattttccac 1200
aaacgcatgc ctccagaagc tgttgatttg gtctcaagac ttcttcaata ctctcctaat 1260
ctacgaagtg ccgctctcga cacattagtc cacccattct ttgatgagtt aagagaccca 1320
aacgcacgtc tacctaatgg acgtttcctt ccaccgcttt tcaacttcaa gcctcacgag 1380
ctgaaaggtg taccattgga gatggtagct aagttagtac ctgagcatgc aaggaagcag 1440
tgtccttggc tcggtttgtg atttcctctt aatgtagcat gaacacaaca aacacttctt 1500
ataaattacc tctctatgta tcaatatgtc acaaactgat atgcaccctt tgtttgttgt 1560
atgagtagag aaaaaaagag ttattactat ggttggttgg ttcataatgt aaaagcccac 1620
caagattttt tatc 1634
<210>153
<211>405
<212>PRT
＜213〉Arabidopis thaliana
<400>153
Met Ala Ser Val Gly Ile Ala Pro Asn Pro Gly Ala Arg Asp Ser Thr
1 5 10 15
Gly Val Asp Lys Leu Pro Glu Glu Met Asn Asp Met Lys Ile Arg Asp
20 25 30
Asp Lys Glu Met Glu Ala Thr Val Val Asp Gly Asn Gly Thr Glu Thr
35 40 45
Gly His Ile Ile Val Thr Thr Ile Gly Gly Arg Asn Gly Gln Pro Lys
50 55 60
Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val Gly His Gly Ser Phe
65 70 75 80
Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly Glu Thr Val Ala
85 90 95
IIe Lys Lys Val Leu Gln Asp Arg Arg Tyr Lys Asn Arg Glu Leu Gln
100 105 110
Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ser Leu Lys His Cys
115 120 125
Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu Asn Leu Val Leu
130 135 140
Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Lys His Tyr Asn Lys
145 150 155 160
Leu Asn Gln Arg Met Pro Leu Ile Tyr Val Lys Leu Tyr Thr Tyr Gln
165 170 175
Ile Phe Arg Ala Leu Ser Tyr Ile His Arg Cys Ile Gly Val Cys His
180 185 190
Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro His Thr His Gln
195 200 205
Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu Val Lys Gly Glu
210 215 220
Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala Pro Glu Leu
225 230 235 240
Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp Val Trp Ser Ala
245 250 255
Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Leu Phe Pro Gly
260 265 270
Glu Ser Gly Val Asp Gln Leu Val Glu IleIle Lys Val Leu Gly Thr
275 280 285
Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn Tyr Thr Glu Phe
290 295 300
Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys Ile Phe His Lys
305 310 315 320
Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg Leu Leu Gln Tyr
325 330 335
Ser Pro Asn Leu Arg Ser Ala Ala Leu Asp Thr Leu Val His Pro Phe
340 345 350
Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro Asn Gly Arg Phe
355 360 365
Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu Leu Lys Gly Val Pro
370 375 380
Leu Glu Met Val Ala Lys Leu Val Pro Glu His Ala Arg Lys Gln Cys
385 390 395 400
Pro Trp Leu Gly Leu
405
<210>154
<211>1669
<212>DNA
＜213〉Arabidopis thaliana
<400>154
cagaccaaca acaaaaagga gataaagaga agaggattca tcatcatcaa tcaatccttc 60
attttatgga tctactcata tcttgattct tccttctatc tctccctttt cttccatctc 120
tttttctctg ggtttccccg atttgaagag cgtgacaaag gaagaatctt ttattaaaac 180
aaattcttct gttttaatct tgggatggcc tcggtgggca tagagcctag tgccgcggtt 240
agagaatcta ctggaaacgt tactgatgct gatagattac ccgaggagat gaaggacatg 300
aaaattcaag atgataaaga aatggaagct acgattgtta atggcaatgt gactgagact 360
ggccatataa tagtaactac tataggagga agaaatggcc agccaaaaca gacaatcagt 420
tacatggcgg agcgagttgt tggacatggc tcctttggtg ttgtgtttca ggccaaatgt 480
ttagaaacag gagaaactgt tgctataaag aaagttctac aagatcggag gtacaagaat 540
cgtgagcttc aaacaatgag gctacttgac catccaaatg ttgtgtcttt gaaacattgt 600
ttcttctcta caaccgaaaa agatgagctt tatctcaact tggttctgga atacgttccg 660
gaaactgtgc accgcgtcat caaacactac aacaaactta accaacgaat gcctctcgtt 720
tacgtcaaac tttacactta tcagattttt aggtccttat cctacattca ccgatgtatc 780
ggcgtatgtc atcgagacat caaacctcaa aacttgttgg taaatccaca cactcatcaa 840
gtgaaactat gcgattttgg aagtgcgaaa gtattggtta aaggagagcc aaacatttca 900
tacatttgct cgaggtatta cagagcacct gagctcattt ttggagccac cgagtatact 960
acagccattg atgtctggtc tgcaggatgt gttctcgccg agcttcttct cgggcagcca 1020
ttgttcccgg gtgagagcgg tgttgatcaa cttgtagaga ttataaaggt tttgggaaca 1080
ccaacaaggg aagaaatcaa atgcatgaac ccgaattaca cagagttcaa atttcctcag 1140
attaaagctc atccatggca taagattttc cacaagagaa tgcctccaga agctgttgat 1200
ttggtctcaa ggcttcttca atactctccc aatctccgtt gtgctgctct tgattcattg 1260
gtccacccat tctttgacga gctaagagat ccgaatgcgc gattacccaa cggacgtttc 1320
cttccaccgc tctttaactt taagcctcat gaacttaaag gtgtgcctgt ggagatggtg 1380
gcgaagttag ttccagaaca tgcgaggaag caatgtccgt ggctcagttt gtgatttgtt 1440
ctcacctgca aacacgaaaa ctagagcaaa gcagtcgaga tattcatctc ttctcttctc 1500
tctccttctc tgtattaata ttattataat gatcatatct caatctgatg atttagtaac 1560
cctttgtttg ttgtatgagt agagaaagag tgaatcattt gtgggggtta tgatattgta 1620
taagccaaca aagattattt tttaaagaga gtttcgtgtt ttctgtctc 1669
<210>155
<211>409
<212>PRT
＜213〉Arabidopis thaliana
<400>155
Met Ala Ser Val Gly Ile Glu Pro Ser Ala Ala Val Arg Glu Ser Thr
1 5 10 15
Gly Asn Val Thr Asp Ala Asp Arg Leu Pro Glu Glu Met Lys Asp Met
20 25 30
Lys Ile Gln Asp Asp Lys Glu Met Glu Ala Thr Ile Val Asn Gly Asn
35 40 45
Val Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly Arg Asn
50 55 60
Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val Gly
65 70 75 80
His Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly
85 90 95
Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Arg Arg Tyr Lys Asn
100 105 110
Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ser
115 120 125
Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu
130 135 140
Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Lys
145 150 155 160
His Tyr Asn Lys Leu Asn Gln Arg Met Pro Leu Val Tyr Val Lys Leu
165 170 175
Tyr Thr Tyr Gln Ile Phe Arg Ser Leu Ser Tyr Ile His Arg Cys Ile
180 185 190
Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro
195 200 205
His Thr His Gln Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu
210 215 220
Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg
225 230 235 240
Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp
245 250 255
Val Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro
260 265 270
Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys
275 280 285
Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn
290 295 300
Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys
305 310 315 320
Ile Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg
325 330 335
Leu Leu Gln Tyr Ser Pro Asn Leu Arg Cys Ala Ala Leu Asp Ser Leu
340 345 350
Val His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro
355 360 365
Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu Leu
370 375 380
Lys Gly Val Pro Val Glu Met Val Ala Lys Leu Val Pro Glu His Ala
385 390 395 400
Arg Lys Gln Cys Pro Trp Leu Ser Leu
405
<210>156
<211>1496
<212>DNA
＜213〉Arabidopis thaliana
<400>156
ctcagatcga tgaagagaag aattaggatt tttacgtttt catcggcttg aaagtttgaa 60
gagttttgta gcctgaaaaa tggcgtcagt gggtatagct cctaatcctg gagcaagaga 120
ctctactggt gttgataaat tgcctgaaga aatgaatgac atgaaaattc gtgacgataa 180
agaaatggaa gcgacagtgg tagatggaaa tggaacagag actggacata tcattgtgac 240
tactattggt ggtagaaatg gccaaccaaa acagacaatt agctacatgg ctgagcgtgt 300
tgttggtcac ggatcttttg gtgttgtgtt ccaagcgaaa tgtcttgaga caggagaaac 360
tgttgcgata aagaaagttt tacaagatag gaggtacaag aaccgtgagc ttcaaaccat 420
gaggctactt gaccatccta atgttgtgtc tctgaaacat tgtttcttct caaccactga 480
aaaagatgag ctttacctca atcttgttct tgagtacgtt ccagaaactg ttcatcgtgt 540
tatcaaacac tacaacaaac tgaatcagag aatgcctctt atatacgtca aactttacac 600
ttatcagatt tttagagcct tatcttacat tcaccgatgc attggtgtgt gtcatcgtga 660
cataaaacct caaaacttgt tggtaaatcc gcacactcat caagtaaagc tatgtgattt 720
tggaagtgca aaagtattgg taaaaggaga accaaacatt tcctacatct gctcgaggta 780
ttacagagca cctgaactta tttttggagc aaccgagtat acgacagcca ttgatgtctg 840
gtctgcagga tgtgttctag ctgaactatt gcttggacag cccttgttcc ctggtgagag 900
cggtgttgat caacttgtac acattatcaa ggtcttggga acgcctacta gagaagaaat 960
caagtgcatg aacccaaact acacggaatt caaattccct cagattaaag ctcatccatg 1020
gcacaagatt ttccacaaac gcatgcctcc agaagctgtt gatttggtct caagacttct 1080
tcaatactct cctaatctac gaagtgccgc tctcgacaca ttagtccacc cattctttga 1140
tgagttaaga gacccaaacg cacgtctacc taatggacgt ttccttccac cggcttttca 1200
cttcaagcct cacgagctga aaggtgtacc attggagatg gtagctaagt tagtacctga 1260
gcatgcaagg aagcagtgtc cttggctcgg tttgtgattt cctcttaatg tagcatgaac 1320
acaacaaaca cttcttataa attacctctc tatgtatcaa tatgtcacaa actgatatgc 1380
accctttgtt tgttgtatga gtagagaaaa aaagagttat tactatggtt ggttggttca 1440
taatgtaaaa gcccaccaag attttttatc tagataaaga gtttgctaaa aaaaaa 1496
<210>157
<211>405
<212>PRT
＜213〉Arabidopis thaliana
<400>157
Met Ala Ser Val Gly Ile Ala Pro Asn Pro Gly Ala Arg Asp Ser Thr
1 5 10 15
Gly Val Asp Lys Leu Pro Glu Glu Met Asn Asp Met Lys Ile Arg Asp
20 25 30
Asp Lys Glu Met Glu Ala Thr Val Val Asp Gly Asn Gly Thr Glu Thr
35 40 45
Gly His Ile Ile Val Thr Thr Ile Gly Gly Arg Asn Gly Gln Pro Lys
50 55 60
Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val Gly His Gly Ser Phe
65 70 75 80
Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly Glu Thr Val Ala
85 90 95
Ile Lys Lys Val Leu Gln Asp Arg Arg Tyr Lys Asn Arg Glu Leu Gln
100 105 110
Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ser Leu Lys His Cys
115 120 125
Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu Asn Leu Val Leu
130 135 140
Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Lys His Tyr Asn Lys
145 150 155 160
Leu Asn Gln Arg Met Pro Leu Ile Tyr Val Lys Leu Tyr Thr Tyr Gln
165 170 175
Ile Phe Arg Ala Leu Ser Tyr Ile His Arg Cys Ile Gly Val Cys His
180 185 190
Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro His Thr His Gln
195 200 205
Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu Val Lys Gly Glu
210 215 220
Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala Pro Glu Leu
225 230 235 240
Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp Val Trp Ser Ala
245 250 255
Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro Leu Phe Pro Gly
260 265 270
Glu Ser Gly Val Asp Gln Leu Val His Ile Ile Lys Val Leu Gly Thr
275 280 285
Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn Tyr Thr Glu Phe
290 295 300
Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys Ile Phe His Lys
305 310 315 320
Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg Leu Leu Gln Tyr
325 330 335
Ser Pro Asn Leu Arg Ser Ala Ala Leu Asp Thr Leu Val His Pro Phe
340 345 350
Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro Asn Gly Arg Phe
355 360 365
Leu Pro Pro Ala Phe His Phe Lys Pro His Glu Leu Lys Gly Val Pro
370 375 380
Leu Glu Met Val Ala Lys Leu Val Pro Glu His Ala Arg Lys Gln Cys
385 390 395 400
Pro Trp Leu Gly Leu
405
<210>158
<211>1712
<212>DNA
＜213〉Arabidopis thaliana
<400>158
attggggtcc gctctctctc tctctctgcc atcagaagaa aaaaccaaac accgagacaa 60
aacacatcac gaagaagaga gagatttgcc attttcggca aggaagtgtt ttattctagg 120
gcttctctag ttgctgcgta gacaagatgg tggagtgaaa ttgcggcggt gacttttcag 180
attgacaaat tgaattaaag atcttgaata catatggctt ctgtgggaac attaccagct 240
tcttctatgg ctacaaaaca aagcaatgct tctatatgcg ctgaaaaatt acctgaaggg 300
attaatgaga tgaagataaa agatgataag gaaatggaag cagctgtggt tgatgggaat 360
ggaactgaaa caggtcacat tattgttaca actattggtg gtaaaaatgg tcagcctaaa 420
cagaccataa gttatatggc agagcgcatc gttggacaag gttcttttgg aatcgttttt 480
caggccaagt gtttagaaac gggggaaact gttgcaatca agaaagtttt gcaagacaag 540
agatacaaga atcgggagct gcagacaatg cgtcttcttg atcaccctaa tgttgtatcc 600
ctcaagcatt gttttttctc aacgactgaa aaagatgagc tctatctcaa tctggtcctt 660
gaatacgttc ctgagactgt ctatcgcgtc tcaaagcact atagtcgggc aaaccagagg 720
atgcccatta tatatgttaa actctatact tatcagattt gcagagcttt ggcttatatt 780
catggtggag taggagtctg ccacagagac ataaaaccac agaatcttct ggttaatcct 840
catacgcatc aggtcaaact atgcgatttt ggtagcgcaa aagttctggt taaaggcgag 900
ccaaacatct catacatctg ctcccgttac tatcgagcac ctgaacttat atttggagct 960
acagaatata caacaacaat tgacatatgg tctgcaggct gtgttcttgc tgaattgctt 1020
ctgggacagc ctctatttcc aggtgagagt ggagttgacc agctagttga gataataaag 1080
gttcttggaa caccaacacg ggaggaaatc aaatgcatga atccaaacta cacagaattc 1140
aaattcccgc aaataaaggc tcatccttgg cacaaaatat tccataagcg tacacctcca 1200
gaagctgtag accttgtctc aagacttctc cagtattctc caaacctcag atcaaccgct 1260
atggaggcga tagttcaccc gttcttcgat gagctacgtg atcccaatac acgtcttcct 1320
aatggtcgtg ccttgcctcc tctcttcaac tttaaacctc aagagctaaa aggagcaagt 1380
ttagagttgt tgtccaagct tatacctgac cacgcccgaa aacaatgttc cttcctcgct 1440
ctctaaatct cttcctctct ctctatatat atgtgtgtgt gtgtgtatgt acacatgcat 1500
ataatatgct tatcgtttct aagtaatgga gatagcttct caggattatc attagctttc 1560
atctttcatg tatctttgtt gtttattgtc ttatcacaac ctttgtactt tattacatac 1620
aatgattagt gtaatgtatg tgacggtctt tgactcgccg gtcgctacag ttatgttgga 1680
tactaaatta taaaataaac ttctcgctcg tc 1712
<210>159
<211>410
<212>PRT
＜213〉Arabidopis thaliana
<400>159
Met Ala Ser Val Gly Thr Leu Pro Ala Ser Ser Met Ala Thr Lys Gln
1 5 10 15
Ser Asn Ala Ser Ile Cys Ala Glu Lys Leu Pro Glu Gly Ile Asn Glu
20 25 30
Met Lys Ile Lys Asp Asp Lys Glu Met Glu Ala Ala Val Val Asp Gly
35 40 45
Asn Gly Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly Lys
50 55 60
Asn Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Ile Val
65 70 75 80
Gly Gln Gly Ser Phe Gly Ile Val Phe Gln Ala Lys Cys Leu Glu Thr
85 90 95
Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys
100 105 110
Asn Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val
115 120 125
Ser Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr
130 135 140
Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val Tyr Arg Val Ser
145 150 155 160
Lys His Tyr Ser Arg Ala Asn Gln Arg Met Pro Ile Ile Tyr Val Lys
165 170 175
Leu Tyr Thr Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Gly Gly
180 185 190
Val Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn
195 200 205
Pro His Thr His Gln Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val
210 215 220
Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr
225 230 235 240
Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Thr Ile
245 250 255
Asp Ile Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln
260 265 270
Pro Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile
275 280 285
Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro
290 295 300
Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His
305 310 315 320
Lys Ile Phe His Lys Arg Thr Pro Pro Glu Ala Val Asp Leu Val Ser
325 330 335
Arg Leu Leu Gln Tyr Ser Pro Asn Leu Arg Ser Thr Ala Met Glu Ala
340 345 350
Ile Val His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg Leu
355 360 365
Pro Asn Gly Arg Ala Leu Pro Pro Leu Phe Asn Phe Lys Pro Gln Glu
370 375 380
Leu Lys Gly Ala Ser Leu Glu Leu Leu Ser Lys Leu Ile Pro Asp His
385 390 395 400
Ala Arg Lys Gln Cys Ser Phe Leu Ala Leu
405 410
<210>160
<211>1230
<212>DNA
＜213〉Arabidopis thaliana
<400>160
atggcctcgg tgggcataga gcctagtgcc gcggttagag aatctactgg aaacgttact 60
gatgctgata gattacccga ggagatgaag gacatgaaaa ttcaagatga taaagaaatg 120
gaagctacga ttgttaatgg caatgtgact gagactggcc atataatagt aactactata 180
ggaggaagaa atggccagcc aaaacagaca atcagttaca tggcggagcg agttgttgga 240
catggctcct ttggtgttgt gtttcaggcc aaatgtttag aaacaggaga aactgttgct 300
ataaagaaag ttctacaaga tcggaggtac aagaatcgtg agcttcaaac aatgaggcta 360
cttgaccatc caaatgttgt gtctttgaaa cattgtttct tctctacaac cgaaaaagat 420
gagctttatc tcaacttggt tctggaatac gttccggaaa ctgtgcaccg cgtcatcaaa 480
cactacaaca aacttaacca acgaatgcct ctcgtttacg tcaaacttta cacttatcag 540
atttttaggt ccttatccta cattcaccga tgtatcggcg tatgtcatcg agacatcaaa 600
cctcaaaact tgttggtaaa tccacacact catcaagtga aactatgcga ttttggaagt 660
gcgaaagtat tggttaaagg agagccaaac atttcataca tttgctcgag gtattacaga 720
gcacctgagc tcatttttgg agccaccgag tatactacag ccattgatgt ctggtctgca 780
ggatgtgttc tcgccgagct tcttctcggg cagccattgt tcccgggtga gagcggtgtt 840
gatcaacttg tagagattat aaaggttttg ggaacaccaa caagggaaga aatcaaatgc 900
atgaacccga attacacaga gttcaaattt cctcagatta aagctcatcc atggcataag 960
attttccaca agagaatgcc tccagaagct gttgatttgg tctcaaggct tcttcaatac 1020
tctcccaatc tccgttgtgc tgctcttgat tcattggtcc acccattctt tgacgagcta 1080
agagatccga atgcgcgatt acccaacgga cgtttccttc caccgctctt taactttaag 1140
cctcatgaac ttaaaggtgt gcctgtggag atggtggcga agttagttcc agaacatgcg 1200
aggaagcaat gtccgtggct cagtttatga 1230
<210>161
<211>409
<212>PRT
＜213〉Arabidopis thaliana
<400>161
Met Ala Ser Val Gly Ile Glu Pro Ser Ala Ala Val Arg Glu Ser Thr
1 5 10 15
Gly Asn Val Thr Asp Ala Asp Arg Leu Pro Glu Glu Met Lys Asp Met
20 25 30
Lys Ile Gln Asp Asp Lys Glu Met Glu Ala Thr Ile Val Asn Gly Asn
35 40 45
Val Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly Arg Asn
50 55 60
Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val Gly
65 70 75 80
His Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly
85 90 95
Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Arg Arg Tyr Lys Asn
100 105 110
Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ser
115 120 125
Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu
130 135 140
Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Lys
145 150 155 160
His Tyr Asn Lys Leu Asn Gln Arg Met Pro Leu Val Tyr Val Lys Leu
165 170 175
Tyr Thr Tyr Gln Ile Phe Arg Ser Leu Ser Tyr Ile His Arg Cys Ile
180 185 190
Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro
195 200 205
His Thr His Gln Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu
210 215 220
Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg
225 230 235 240
Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp
245 250 255
Val Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro
260 265 270
Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys
275 280 285
Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn
290 295 300
Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys
305 310 315 320
Ile Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg
325 330 335
Leu Leu Gln Tyr Ser Pro Asn Leu Arg Cys Ala Ala Leu Asp Ser Leu
340 345 350
Val His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg Leu Pro
355 360 365
Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu Leu
370 375 380
Lys Gly Val Pro Val Glu Met Val Ala Lys Leu Val Pro Glu His Ala
385 390 395 400
Arg Lys Gln Cys Pro Trp Leu Ser Leu
405
<210>162
<211>1967
<212>DNA
＜213〉Zea mays
<400>162
ttgactgcag gcctcggcac ggtaggcaca aatcattttg gccgctgcgt cgcgttggga 60
gcagtattct actgtgcctg cgtcccgaca agcgaaaaga agagacgggg gagggtggga 120
gaacggatac cgaaaggggt aggagagtga gcagccagag aacagggagg tagagaggag 180
gaggaggtag aaggcgaaag aaggggaacc aaatcttgga cgggaacaca tagatttctt 240
tggtggagga aggaggaggg caacaagagg aggttacagg tagcccaata gatctactgc 300
tgttgaggga gttgatgcaa agctgagttg ctgcgcgttg gctttcttta gagatggctt 360
cagctggtgt tgccccttct gggtacaaga acagcagcag caccagcatt ggtgccgaaa 420
agttgcaaga tcacatgaac gagctaaaga ttagagatga taaggaagtt gaagcaacca 480
taattaatgg gaaagggact gaaactgggc acataattgt caccactact ggtggcaaga 540
atggtcaacc aaaacagaca gtgagctaca tggctgagcg cattgtaggt caaggttctt 600
ttgggatcgt cttccaggcc aagtgtttgg aaacgggtga gactgttgcc ataaagaagg 660
ttcttcaaga caagcgttac aagaaccgcg aactgcagac catgcgcctt cttgaccacc 720
ctaatgttgt tgctttgaag cattgcttct tttcaactac tgagaaggat gagctttatc 780
tgaacttggt ccttgagtat gttccggaga cagttcatcg agttgtgaaa catcacaaca 840
agatgcacca acgcatgcca cttatttatg tgaagcttta tatgtaccag atatgtagag 900
cattggctta cattcatggt actatcggtg tctgccacag agatattaag ccacaaaatc 960
ttctggtgaa cccacacacc caccagctta aaatatgtga ctttggtagt gcaaaagttc 1020
tggtcaaggg ggaaccaaac atatcataca tctgctcgcg atactatagg gctccagagc 1080
tcatatttgg tgccactgag tataccacag cgattgacat ttggtctgct ggatgtgttc 1140
ttgctgagct tatgctaggg cagcctttgt ttccgggtga aagtggtgtg gaccaacttg 1200
ttgaaatcat caaggtcctc ggtacgccaa caagggaaga aattaaatgc atgaacccaa 1260
attacacaga gtttaagttc ccacaaatca aagcacaccc atggcacaag gtattccaca 1320
aaaggatgcc gccagaagct gttgatctgg tctctcggct actccagtac tccccaaatc 1380
tgagatgcac tgctatggag gcacttgttc acccattctt tgatgagctt cgagatccta 1440
atactcgcct tccaaatggt cgctttttgc caccactatt caatttcaag cctcacgaac 1500
ttaaaggagt cccatcagac attgttgcga aattggttcc agaacatgcg aagaagcaat 1560
gctcttatgt tggattgtga aatgaccgcg ccttgggact ggaacctgag gtcgcaatcg 1620
tgcatttccc ctgggatgtt ggatgatctt gaggcatgcg agcctgttgt tgaagatgca 1680
aggttacgta cttgtacgac aatgtgacct gtgtagctga gtagtctatg tcgcagtgac 1740
atgtaacggc accccccttc ctactaactg acgcttactc gagattgcca tagttgatct 1800
tgtaatttgt tatagagcag tatgaatgta tttatggtag cttgaatcta tgtatggatt 1860
cacttcgttt ttccatgttt ccttgtctcc agacccagat tgctaccgta ttgtttcaga 1920
attcctagct acctgttgcc taaaaaaaaa aaaaaaaaac ctcgtgc 1967
<210>163
<211>408
<212>PRT
＜213〉Zea mays
<400>163
Met Ala Ser Ala Gly Val Ala Pro Ser Gly Tyr Lys Asn Ser Ser Ser
1 5 10 15
Thr Ser Ile Gly Ala Glu Lys Leu Gln Asp His Met Asn Glu Leu Lys
20 25 30
Ile Arg Asp Asp Lys Glu Val Glu Ala Thr Ile Ile Asn Gly Lys Gly
35 40 45
Thr Glu Thr Gly His Ile Ile Val Thr Thr Thr Gly Gly Lys Asn Gly
50 55 60
Gln Pro Lys Gln Thr Val Ser Tyr Met Ala Glu Arg Ile Val Gly Gln
65 70 75 80
Gly Ser Phe Gly Ile Val Phe Gln Ala Lys Cys Leu Glu Thr Gly Glu
85 90 95
Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys Asn Arg
100 105 110
Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val Ala Leu
115 120 125
Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr Leu Asn
130 135 140
Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Val Lys His
145 150 155 160
His Asn Lys Met His Gln Arg Met Pro Leu Ile Tyr Val Lys Leu Tyr
165 170 175
Met Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Gly ThrIle Gly
180 185 190
Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro His
195 200 205
Thr His Gln Leu Lys Ile Cys Asp Phe Gly Ser Ala Lys Val Leu Val
210 215 220
Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg Ala
225 230 235 240
Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp Ile
245 250 255
Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Met Leu Gly Gln Pro Leu
260 265 270
Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys Val
275 280 285
Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn Tyr
290 295 300
Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys Val
305 310 315 320
Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg Leu
325 330 335
Leu Gln Tyr Ser Pro Asn Leu Arg Cys Thr Ala Met Glu Ala Leu Val
340 345 350
His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg Leu Pro Asn
355 360 365
Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu Leu Lys
370 375 380
Gly Val Pro Ser Asp Ile Val Ala Lys Leu Val Pro Glu His Ala Lys
385 390 395 400
Lys Gln Cys Ser Tyr Val Gly Leu
405
<210>164
<211>1752
<212>DNA
＜213〉Zea mays
<400>164
gcacgagccg cgccggagtt ggaggaggga gaggggacaa gctttccggc gccgacgccg 60
acgcggaccc ggcgccgaca cgatccggtg gatcaagtgc atcacacctt tagggaggcc 120
ccttggacag cagtttgtgc tgcaaattct atatagctct gtcgcagcat ggcctcggtg 180
ggcgtggcac gctcttcttt gggatttcag aatggcacaa gttctagcag tgacccagat 240
cgtcttccca acgagttggg cagtatgagc ataagggacg acaaggacgt tgaagatatt 300
gtagtcaatg gcaatggggc ggagcctggt catatcatag tgaccagcat tgatgggaga 360
aatgggcagg caaagcagac cattagttac atggctgagc gggtggtagg tcatgggtcc 420
ttcggaaccg ttttccaggc caagtgtctt gaaactggtg agaccgtagc tataaaaaag 480
gttcttcaag acaagagata caagaatcgt gagctgcaaa ccatgcgagt gcttgaccac 540
ccaaatgtgg tggctctaaa gcactgtttc ttctcaaaga ctgagaaaga ggagctttac 600
ctcaatttgg tgcttgagta tgtaccggag actgctcatc gtgtcatcaa acattacaac 660
aagatgaacc agcgcatgcc tttgatttat gcaaaactgt atatgtatca gatttgtaga 720
gccttggcat acattcacaa cagcattgga gtgtgccaca gggacattaa gccgcaaaat 780
ctcctggtta atcctcatac ccatcagcta aaattgtgtg actttggcag cgcgaaagtt 840
ctggtaaaag gcgaaccaaa catttcttac atctgttcta ggtactacag agctccagag 900
ctcatatttg gtgctactga atacacaaca gccattgatg tttggtctgc tggctgtgtg 960
ctcgctgagc tgcttctagg acagcctctg ttccctggag aaagcggtgt tgatcagctt 1020
gttgaaatca tcaaggttct gggcacaccc acacgtgaag aaattaagtg catgaatcca 1080
aattataccg agtttaaatt cccgcaaatc aaagctcacc catggcataa gatattccat 1140
aaaaggatgc ctgctgaagc ggtagatctc gtgtccaggc ttctgcagta ctcaccaaaa 1200
cttcggtcga ctgctttgga agcattggtc catccgttct ttgatgaact tcgggatcca 1260
aacacccgct taccgaatgg tcgttttctt ccgcctctct tcaattttaa gccccatgag 1320
ctgaagaacg tgccggcgga tttcatggtg aaattggtcc ctgagcatgc acggaagcaa 1380
tgtgccttcg tagggtggtg atctctggat aagaggatga cgactcgatg attagctgag 1440
gaccaagtta atgtctgtta gaaactgccg gagatcgaca ttgccagatg tggtgtggta 1500
taagataggc aatatgtgtg attatttttt gttcgaggtt atcacccccc ttgccccaga 1560
aaagatgaga agatgtcgat gtaacaagcc ctctgcgctt ctgtaagtag atgagtgttg 1620
ctgcatgccc cctgggtaca tgtatcggtt tgagcagaat tctgtttgcc tgaatcgtgc 1680
catcaccacg cagggatcca tcccttgtgt gacgatgttc agcccaaaaa aaaaaaaaaa 1740
aaaaaaaaaa aa 1752
<210>165
<211>410
<212>PRT
＜213〉Zea mays
<400>165
Met Ala Ser Val Gly Val Ala Arg Ser Ser Leu Gly Phe Gln Asn Gly
1 5 10 15
Thr Ser Ser Ser Ser Asp Pro Asp Arg Leu Pro Asn Glu Leu Gly Ser
20 25 30
Met Ser Ile Arg Asp Asp Lys Asp Val Glu Asp Ile Val Val Asn Gly
35 40 45
Asn Gly Ala Glu Pro Gly His Ile Ile Val Thr Ser Ile Asp Gly Arg
50 55 60
Asn Gly Gln Ala Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val
65 70 75 80
Gly His Gly Ser Phe Gly Thr Val Phe Gln Ala Lys Cys Leu Glu Thr
85 90 95
Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys
100 105 110
Asn Arg Glu Leu Gln Thr Met Arg Val Leu Asp His Pro Asn Val Val
115 120 125
Ala Leu Lys His Cys Phe Phe Ser Lys Thr Glu Lys Glu Glu Leu Tyr
130 135 140
Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Ala His Arg Val Ile
145 150 155 160
Lys His Tyr Asn Lys Met Asn Gln Arg Met Pro Leu Ile Tyr Ala Lys
165 170 175
Leu Tyr Met Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Asn Ser
180 185 190
Ile Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn
195 200 205
Pro His Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Val
210 215 220
Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr
225 230 235 240
Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile
245 250 255
Asp Val Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln
260 265 270
Pro Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile
275 280 285
Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro
290 295 300
Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His
305 310 315 320
Lys Ile Phe His Lys Arg Met Pro Ala Glu Ala Val Asp Leu Val Ser
325 330 335
Arg Leu Leu Gln Tyr Ser Pro Lys Leu Arg Ser Thr Ala Leu Glu Ala
340 345 350
Leu Val His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg Leu
355 360 365
Pro Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His Glu
370 375 380
Leu Lys Asn Val Pro Ala Asp Phe Met Val Lys Leu Val Pro Glu His
385 390 395 400
Ala Arg Lys Gln Cys Ala Phe Val Gly Trp
405 410
<210>166
<211>1800
<212>DNA
＜213〉alfalfa
<400>166
tttttttttt tttttttttt tcaaaatatt ttccatttgt ctcttctttc ttctttccta 60
attccgaatc ccacggattt cacttccatt caaagtcata gctagatcca atccattcca 120
ttcctctgtt tgagttgaag agttgatttg ggggttggat gttcagtttt gaagctgtgt 180
atgatctgaa aggaaataat taagtaaagt gttttgcact ttttttaaga agatatgatg 240
gcatcaggtg gtgttgcacc tgcttctgga tttatagata agaatgcaag ttcagttggt 300
gttgaaaagt tgcctgagga gatgaatgac atgaaaatta gggatgataa ggaaatggaa 360
gccgctacca ttgtagatgg aaatgggacc gaaaccggac atataattgt cacaaccatt 420
ggtggtaaaa atggccagcc aaagcagaca ataagttaca tggccgagcg tgttgttgga 480
catggatctt ttggtgtagt ttttcaggca aagtgtttgg agactggaga aactgtggct 540
ataaagaagg ttcttcaaga taagaggtac aagaaccggg aattgcaaac tatgcgcctt 600
ctggaccacc ctaatgttgt atctttgaag cactgcttct tctcaacgac tgaaaaggac 660
gagctttatc ttaacctggt gcttgaatat gttcctgaga ctgtcagccg tgtgattaga 720
cactacaaca aaatgaatca aagaatgcct atgatatatg tcaaacttta ttcttaccag 780
atttgcaggg cacttgctta tattcacaac agtattggag tatgtcacag ggatattaaa 840
cctcaaaatt tactggtcaa tcctcacacc caccaactga agatatgcga ctttggaagt 900
gctaaagtct tggtaaaagg tgaaccaaac atatcttaca tctgttctag gtactataga 960
gctcctgagc ttatattcgg tgcaactgag tacaccacag ccattgacat ctggtcagct 1020
ggttgcgtac ttggtgaact tttgcttggc cagccactgt ttcctggtga gagtggagta 1080
gaccaacttg tggaaattat caaggtttta ggcaccccaa caagggaaga aatcaagtgc 1140
atgaatccta attatacaga gtttaaattt cctcaaatca aagctcatcc atggcataag 1200
atttttcaca agagaatgcc tcctgaagct gtggatcttg tctcaagact attgcaatac 1260
tctccaaatc ttcgaagcac agctttggag gctttggttc atccattcta tgatgacgtg 1320
cgcgatccaa acactcggtt gccaaatggg cgtttccttc caccattatt taacttcaaa 1380
gtcaatgagc tcaagggagt acctgcagag atgctggtga aactggttcc acctcatgca 1440
agaaagcaat gtgccttgtt cgggtcatca tgaagcagcc ttgtgtagtt attaagtact 1500
ttcttctacc tatgtaaagg tgtatctagt caaatttcaa gtggttaaat aggttatttc 1560
tattttcttt ctgtttgatt tgttctcacc caacctacca tccaatttat tattattttt 1620
cttcctcgat gtagaagaaa gctgtgctgt ttaagaagca acttcagctt gattattact 1680
ttgtaggatc tgatgtgttc actcacctta acatgaacca ttgtttattg aagtgataga 1740
cttgatcaca acctcactga actagtagga gatgtttcaa atcttgaaaa aaaaaaaaaa 1800
<210>167
<211>412
<212>PRT
＜213〉alfalfa
<400>167
Met Met Ala Ser Gly Gly Val Ala Pro Ala Ser Gly Phe Ile Asp Lys
1 5 10 15
Asn Ala Ser Ser Val Gly Val Glu Lys Leu Pro Glu Glu Met Asn Asp
20 25 30
Met Lys Ile Arg Asp Asp Lys Glu Met Glu Ala Ala Thr Ile Val Asp
35 40 45
Gly Asn Gly Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly
50 55 60
Lys Asn Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val
65 70 75 80
Val Gly His Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu
85 90 95
Thr Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr
100 105 110
Lys Asn Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val
115 120 125
Val Ser Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu
130 135 140
Tyr Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val Ser Arg Val
145 150 155 160
Ile Arg His Tyr Asn Lys Met Asn Gln Arg Met Pro Met Ile Tyr Val
165 170 175
Lys Leu Tyr Ser Tyr Gln Ile Cys Arg Ala Leu Ala Tyr Ile His Asn
180 185 190
Ser Ile Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val
195 200 205
Asn Pro His Thr His Gln Leu Lys Ile Cys Asp Phe Gly Ser Ala Lys
210 215 220
Val Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr
225 230 235 240
Tyr Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala
245 250 255
Ile Asp Ile Trp Ser Ala Gly Cys Val Leu Gly Glu Leu Leu Leu Gly
260 265 270
Gln Pro Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile
275 280 285
Ile Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn
290 295 300
Pro Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp
305 310 315 320
His Lys Ile Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val
325 330 335
Ser Arg Leu Leu Gln Tyr Ser Pro Asn Leu Arg Ser Thr Ala Leu Glu
340 345 350
Ala Leu Val His Pro Phe Tyr Asp Asp Val Arg Asp Pro Asn Thr Arg
355 360 365
Leu Pro Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Val Asn
370 375 380
Glu Leu Lys Gly Val Pro Ala Glu Met Leu Val Lys Leu Val Pro Pro
385 390 395 400
His Ala Arg Lys Gln Cys Ala Leu Phe Gly Ser Ser
405 410
<210>168
<211>1613
<212>DNA
＜213〉alfalfa
<400>168
gagcacatgc tttggtttcg tttcgttggt ggatgagtgg ttaaaattga aagggtttca 60
gtggcaatgg cgtcggttgg tgttgcacca acttcaggtt ttagagaagt ccttggtgat 120
ggtgaaattg gtgttgatga tatattgcca gaggaaatga gtgatatgaa aattagggat 180
gatagagaaa tggaagccac cgttgttgac ggcaatggaa cggagacagg acatatcatt 240
gtcactacta ttggtggtag aaatggtcag ccaaagcaga ctataagcta tatggcagag 300
cgtgttgtag gacatggatc atttggagtt gtcttccagg ctaagtgctt ggaaactggt 360
gaaaccgtgg ctatcaaaaa ggttcttcaa gacaagaggt acaagaaccg ggaattgcaa 420
acaatgcgac tgcttgatca cccgaatgtc gtctctttaa agcattgttt cttttcaacc 480
accgaaaagg atgaactata cctgaatttg gtacttgagt atgttcctga aacagttcat 540
cgcgtgatta agcattacag caagttgaac caaaggatgc caatgattta tgtgaagctc 600
tatacatacc agatctttag agcattatct tatattcatc gttgcattgg agtctgtcat 660
cgggatatca agccccaaaa tctattggtc aatccacaca cccaccaggt taaattatgc 720
gactttggaa gtgcgaaagt cttggttaaa ggcgaaccaa atatatcgta tatatgttct 780
agatactaca gagcacccga gcttattttt ggagcaactg aatatactac tgctattgat 840
gtatggtctg ttggttgtgt tttggctgag ctgctgcttg gacagccatt gttcccaggt 900
gagagaggag ttgatcagct tgttgagatc atcaaggttc tgggaactcc gacaagagaa 960
gaaattaaat gcatgaatcc taattatacc gaatttaaat tccctcaaat caaagcacat 1020
ccatggcaca agatcttcca taagcgcatg cctgcagaag ctgttgattt ggtatcaaga 1080
ttattacaat actccccaaa cctgcggtgc caagctttag attgcttgac ccatcctttc 1140
ttcgatgagc ttcgtgaccc aaatgctcgc ttgccaactg gccgtttcct cccaccactg 1200
tttaacttca aacctcacga actgaaagga gttccagtcg agaccttgat gaaactggtt 1260
ccagagcatg cgaggaagca atgcccgttt cttggcttgt aatatgtcgt aaaatgtaac 1320
aaaactgcaa gtgttgtttc catatgaacg ttctatttga tgatatgata tttattagta 1380
tctttgttgt attcggttgc ctgtgataga aaatttagag atatatgcta cccaatatta 1440
cccaaaccct tatatgggta ttcagaatac ccttttcctg tatcacagca gattgtaaca 1500
tgcaatagaa gacaagtgtc tacaattatc taaatgttgt atcagtattt gtacttgtat 1560
ttgtatttgt ggagataatg acggattatt gcgtaaaaaa aaaaaaaaaa aaa 1613
<210>169
<211>411
<212>PRT
＜213〉alfalfa
<400>169
Met Ala Ser Val Gly Val Ala Pro Thr Ser Gly Phe Arg Glu Val Leu
1 5 10 15
Gly Asp Gly Glu Ile Gly Val Asp Asp Ile Leu Pro Glu Glu Met Ser
20 25 30
Asp Met Lys Ile Arg Asp Asp Arg Glu Met Glu Ala Thr Val Val Asp
35 40 45
Gly Asn Gly Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly
50 55 60
Arg Asn Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val
65 70 75 80
Val Gly His Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu
85 90 95
Thr Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr
100 105 110
Lys Asn Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val
115 120 125
Val Ser Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu
130 135 140
Tyr Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val
145 150 155 160
Ile Lys His Tyr Ser Lys Leu Asn Gln Arg Met Pro Met Ile Tyr Val
165 170 175
Lys Leu Tyr Thr Tyr Gln Ile Phe Arg Ala Leu Ser Tyr Ile His Arg
180 185 190
Cys Ile Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val
195 200 205
Asn Pro His Thr His Gln Val Lys Leu Cys Asp Phe Gly Ser Ala Lys
210 215 220
Val Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr
225 230 235 240
Tyr Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala
245 250 255
Ile Asp Val Trp Ser Val Gly Cys Val Leu Ala Glu Leu Leu Leu Gly
260 265 270
Gln Pro Leu Phe Pro Gly Glu Arg Gly Val Asp Gln Leu Val Glu Ile
275 280 285
Ile Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn
290 295 300
Pro Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp
305 310 315 320
His Lys Ile Phe His Lys Arg Met Pro Ala Glu Ala Val Asp Leu Val
325 330 335
Ser Arg Leu Leu Gln Tyr Ser Pro Asn Leu Arg Cys Gln Ala Leu Asp
340 345 350
Cys Leu Thr His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Ala Arg
355 360 365
Leu Pro Thr Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys Pro His
370 375 380
Glu Leu Lys Gly Val Pro Val Glu Thr Leu Met Lys Leu Val Pro Glu
385 390 395 400
His Ala Arg Lys Gln Cys Pro Phe Leu Gly Leu
405 410
<210>170
<211>1543
<212>DNA
＜213〉alfalfa
<400>170
gttcaattga agctgcaaaa aaaaaaaaaa tcttgatcta ctttggttgt gataatggca 60
acagcgggtg tagcacctgc ttctggaata gtagatgtaa atgcaagttc agctattgct 120
gttgataagt tacctgatga gattcttggc atgagaatta aggatgataa ggaaatggaa 180
gcacatgtgg tagatggaaa tagtactgaa gcaggacatg taattgtcac taccattggt 240
ggtaaaaatg gccagccaaa gcagacaata agctacatgg ctgagcgtgc tgttggacag 300
ggatcatttg gtgtagtttt ccaggctaag tgcttggaga caggtgaaac tgtggctata 360
aaaaaggttc ttcaagacaa gaggtataag aaccgggaat tgcaaacaat gcgccttctg 420
gaccacccca atgttgtaac tttgaagcat tgtttctttt caacaactga aaaagacgag 480
ctctatctta acttggtact tgagtttgtt cctgagactg tccatcgtgt gatcagacac 540
tacagcaaaa tgaatcagag gatgccattg atatacgtaa aactttattc ttaccagata 600
tgtagatcac tagcttatat tcataactgt gttggagtgt ctcataggga cataaaacct 660
caaaatttac tggtcaatcc tcacacccat cagctgaagc tgtgtgactt tgggagtgca 720
aaagtcttgg tcaagggtga accaaacata tcttacatct gttcgaggta ttatagagct 780
cctgagctta tatttggtgc aactgaatac acctcagcca ttgacatttg gtcagctggc 840
tgtgtgcttg gcgaactatt gcttggccag cctctctttc ccggtgcgag tggagtagac 900
cagctcgttg aaattatcaa ggttttaggt accccaacaa gggaagaaat aaagtgtatg 960
aatcctaatt acactgagtt caaattccca caaatcaaag ctcatccatg gcacaagatc 1020
tttcgcaagc gtatgccacc ggaagctgtg gatctcgtct caagactact tcaatactct 1080
ccaaatcttc gaagcacagc tttggaggct ctggttcatc ccttctttga tgaattgcgt 1140
gatccaaata cccgcttacc aaatgggcga catcttcctc ctttatttaa cttcaaagcc 1200
aacgagctta agggagtgcc tgctgaaatg ctggtgaagt tggttccgtc tcacgcaaga 1260
aagcagtgtt ctttgtttgc gtcgtcatag acttagaatg ctgtcttgtg taaatattat 1320
ggactccctg tttgtagaat tgtatgtagc ctgtttcatg ttgttaatag tctctctttt 1380
gttatttgtt ccattaattt gtttctacca aagaaaacca cctcagttta ttataagttt 1440
caagttgatc tctcttcacg atttaattgt tctcctgatc ttcaattatt aacagattgt 1500
attaactatt aactattaac tagaacaatt gtttattcta gcg 1543
<210>171
<211>411
<212>PRT
＜213〉alfalfa
<400>171
Met Ala Thr Ala Gly Val Ala Pro Ala Ser Gly Ile Val Asp Val Asn
1 5 10 15
Ala Ser Ser Ala Ile Ala Val Asp Lys Leu Pro Asp Glu Ile Leu Gly
20 25 30
Met Arg Ile Lys Asp Asp Lys Glu Met Glu Ala His Val Val Asp Gly
35 40 45
Asn Ser Thr Glu Ala Gly His Val Ile Val Thr Thr Ile Gly Gly Lys
50 55 60
Asn Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Ala Val
65 70 75 80
Gly Gln Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr
85 90 95
Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys
100 105 110
Asn Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val
115 120 125
Thr Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr
130 135 140
Leu Asn Leu Val Leu Glu Phe Val Pro Glu Thr Val His Arg Val Ile
145 150 155 160
Arg His Tyr Ser Lys Met Asn Gln Arg Met Pro Leu Ile Tyr Val Lys
165 170 175
Leu Tyr Ser Tyr Gln Ile Cys Arg Ser Leu Ala Tyr Ile His Asn Cys
180 185 190
Val Gly Val Ser His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn
195 200 205
Pro His Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Val
210 215 220
Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr
225 230 235 240
Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Ser Ala Ile
245 250 255
Asp Ile Trp Ser Ala Gly Cys Val Leu Gly Glu Leu Leu Leu Gly Gln
260 265 270
Pro Leu Phe Pro Gly Ala Ser Gly Val Asp Gln Leu Val Glu Ile Ile
275 280 285
Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro
290 295 300
Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His
305 310 315 320
Lys Ile Phe Arg Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser
325 330 335
Arg Leu Leu Gln Tyr Ser Pro Asn Leu Arg Ser Thr Ala Leu Glu Ala
340 345 350
Leu Val His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg Leu
355 360 365
Pro Asn Gly Arg His Leu Pro Pro Leu Phe Asn Phe Lys Ala Asn Glu
370 375 380
Leu Lys Gly Val Pro Ala Glu Met Leu Val Lys Leu Val Pro Ser His
385 390 395 400
Ala Arg Lys Gln Cys Ser Leu Phe Ala Ser Ser
405 410
<210>172
<211>1724
<212>DNA
＜213〉tobacco
<400>172
tctctctcat cttccctgcg attctctctc tcaatttcag gttttctctg tttctgtgcg 60
agcctctaat gatcggtgta ttctcaatat cctgaagatt ttctgatttc atcggtgaat 120
gactttttga ggtgataata gttcgcaaaa tttcaggaaa tgacttcagt aggcttagca 180
cctgtatccg gtttgagaga atccagtagc catagtgttg gtgtagatag gctgcctgag 240
gagatgaatg acatgagaat cagggatgat aaggaaatcg aagcagctat tgtggatggt 300
aatgggactg agacaggcca tataatagtg acaactattg gtggtagaca tggtcagcca 360
aaacagacta tcagttatat ggctgaacgt attgttggac aaggatcatt tggagtggtt 420
ttccaggcaa aatgcttaga gactggtgaa actgttgcta ttaaaaaggt tcttcaagac 480
aagagatata agaacaggga gctgcagacc atgcgtcttc ttgatcaccc aaatgttgtg 540
tgcctgaagc actgcttctt ttcaacaact gagaaggatg aagtatatct taatttggtt 600
cttgaatacg tccctgaaac tgtccatcgt gttattaaac actacaataa gttgaatcaa 660
aggatgccat tgatactagt gaagctttat acatatcaga ttttcagggc attgtcttac 720
atccatcaca caattggagt gtgccacagg gacataaagc ctcagaatct tttggtgaat 780
ccacatactc accaggttaa attgtgtgac tttggaagtg ctaaagttct ggttaaagga 840
gaaccaaata tttcttacat ctgctctagg tattatagag cgcctgaact tatatttgga 900
gcaacagagt acactaccgc tattgacatc tggtctgctg gctgtgttct agccgagcta 960
cttcttgggc agcctttgtt tccgggtgaa agtggagttg atcagcttgt tgagattatt 1020
aaggtcttgg gtactcctac cagggaagaa attaaatgca tgaatccaaa ttataacgag 1080
ttcaaattcc cccaaattaa agctcatccg tggcacaaga tatttcacaa gcgcatgcct 1140
ccagaagctg ttgatctggt ttcaagacta ctgcagtact cacctaactt gcgttgcact 1200
gctttagagg cagtgaccca tgccttcttc gatgagcttc gtgatcctaa tacacgcctc 1260
ccaaatggcc gcgtccttcc ccccttgttt aactttaagg cccatgagtt aaagggtgtg 1320
tctgcagaga atctattgaa gttggttccg gagcatgcca ggaaacagtg cccgtccctt 1380
ggtttatgag ttcccactgt acggtagata taatttaagt gtaagctatg ttatttctct 1440
gtatccattt ttcccccttt gctccccaca tgtaccagtt gtctctttgt attattatcc 1500
tagtttgtaa aagcagaggt aggatgtggt ctttaacatt ccttacctcc aacactttcc 1560
tttcaccctg ttccttttat gtcctactgt tgtaactttt atgtggttaa gggtggactg 1620
ctttctatat gaactattat tttatgatga aatttcaaag tgatttttag tgaaaaaaaa 1680
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 1724
<210>173
<211>409
<212>PRT
＜213〉tobacco
<400>173
Met Thr Ser Val Gly Leu Ala Pro Val Ser Gly Leu Arg Glu Ser Ser
1 5 10 15
Ser His Ser Val Gly Val Asp Arg Leu Pro Glu Glu Met Asn Asp Met
20 25 30
Arg Ile Arg Asp Asp Lys Glu Ile Glu Ala Ala Ile Val Asp Gly Asn
35 40 45
Gly Thr Glu Thr Gly His Ile Ile Val Thr Thr Ile Gly Gly Arg His
50 55 60
Gly Gln Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Ile Val Gly
65 70 75 80
Gln Gly Ser Phe Gly Val Val Phe Gln Ala Lys Cys Leu Glu Thr Gly
85 90 95
Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys Asn
100 105 110
Arg Glu Leu Gln Thr Met Arg Leu Leu Asp His Pro Asn Val Val Cys
115 120 125
Leu Lys His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Val Tyr Leu
130 135 140
Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val His Arg Val Ile Lys
145 150 155 160
His Tyr Asn Lys Leu Asn Gln Arg Met Pro Leu Ile Leu Val Lys Leu
165 170 175
Tyr Thr Tyr Gln Ile Phe Arg Ala Leu Ser Tyr Ile His His Thr Ile
180 185 190
Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro
195 200 205
His Thr His Gln Val Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu
210 215 220
Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg
225 230 235 240
Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Thr Ala Ile Asp
245 250 255
Ile Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro
260 265 270
Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys
275 280 285
Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys Met Asn Pro Asn
290 295 300
Tyr Asn Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys
305 310 315 320
Ile Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg
325 330 335
Leu Leu Gln Tyr Ser Pro Asn Leu Arg Cys Thr Ala Leu Glu Ala Val
340 345 350
Thr His Ala Phe Phe Asp Glu Leu Arg Asp Pro Asn Thr Arg Leu Pro
355 360 365
Asn Gly Arg Val Leu Pro Pro Leu Phe Asn Phe Lys Ala His Glu Leu
370 375 380
Lys Gly Val Ser Ala Glu Asn Leu Leu Lys Leu Val Pro Glu His Ala
385 390 395 400
Arg Lys Gln Cys Pro Ser Leu Gly Leu
405
<210>174
<211>1573
<212>DNA
＜213〉wheat
<400>174
gttggtgtgg tgcgtccttc ctcgcgcttt cagaacgaca cgagtactag tggtgatgcc 60
gaccgacttc cgaacgagat gggcaatatg agcataaggg atgacaggga ccctgaggat 120
atagtagtca acggcaatgg gacggaacca ggccatatta tagtcacaag cattgaggga 180
agaaatgggc aagcaaaaca gaccattagc tacatggctg agcgtgtggt tggtaatggg 240
tcatttggaa ctgttttcca ggctaagtgt cttgaaactg gcgagacggt ggctataaag 300
aaggttcttc aagacaagag atataagaac cgtgagctgc aaacgatgcg agttcttgac 360
cacccaaatg ttgtggcttt aaagcattgt tttttctcaa agactgagaa agaggagctt 420
tacctcaacc tggtgcttga gtatgtgccg gagactgctc atcgtgtcat taagcattat 480
aacaagatga accaacgcat gccattgata tatgcaaaac tgtacatgta tcagatatgt 540
agatctttgg catacattca caacagcatt ggagtatgcc acagagacat caagcctcaa 600
aatcttctgg tgaatccaca tacgcaccaa ttgaaattat gtgacttcgg aagtgcgaaa 660
gtgttggtaa aaggagaacc aaatatttcc tatatctgtt caaggtacta tagagcccca 720
gagctcatat ttggtgctac tgaatacaca acggcaattg acgtttggtc tgctggctgt 780
gttcttgctg aactccttct aggacagcct atattccctg gcgacagtgg tgttgatcag 840
cttgttgaaa tcatcaaggt tttaggtacc cctacaagag aagaaattaa gtgcatgaat 900
ccaaattata cggagtttaa attcccacaa atcaaagctc acccatggca caagatcttc 960
cataaaagaa tgcctgctga agcagtagat cttgtctcca gactcttgca atattcacca 1020
agcctgcgtt caactgcttt ggaagcatta attcatccat tcttcgatga actccgggac 1080
ccaaacaccc gtttgccgaa cggccgtttt cttcctcccc tctttaactt taagccccat 1140
gagttgaagg gtgtgccgat ggacatcctg gtgaagctca tccctgaaca tgctcggaag 1200
aactgtgcct ttgtaggatg gtgatccgcc agacggctgc ttgaagttta gttcagaaca 1260
aatccagttg ttgtctacta gaaaccccag gagtttgaga ttgtctgcag ccacacggga 1320
tataggcgat gacacatgtg attattattc cttttctcgt ccgagacctc gatgccatgt 1380
attctttccc cctactgccg atgtaacaaa ccacccatga tactgtaagt agatgagaag 1440
tgtttcgacc gttttcccct gagctcatgt gctatgcaat gaaggatgca ccctatgtac 1500
cgccaatatt tggtccagta tttgttcatg gatcgaggcc cccaaaaaaa aaaaaaaaaa 1560
aaaaaaaaaa aaa 1573
<210>175
<211>381
<212>PRT
＜213〉wheat
<400>175
Met Gly Asn Met Ser Ile Arg Asp Asp Arg Asp Pro Glu Asp Ile Val
1 5 10 15
Val Asn Gly Asn Gly Thr Glu Pro Gly His Ile Ile Val Thr SerIle
20 25 30
Glu Gly Arg Asn Gly Gln Ala Lys Gln Thr Ile Ser Tyr Met Ala Glu
35 40 45
Arg Val Val Gly Asn Gly Ser Phe Gly Thr Val Phe Gln Ala Lys Cys
50 55 60
Leu Glu Thr Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys
65 70 75 80
Arg Tyr Lys Asn Arg Glu Leu Gln Thr Met Arg Val Leu Asp His Pro
85 90 95
Asn Val Val Ala Leu Lys His Cys Phe Phe Ser Lys Thr Glu Lys Glu
100 105 110
Glu Leu Tyr Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Ala His
115 120 125
Arg Val Ile Lys His Tyr Asn Lys Met Asn Gln Arg Met Pro Leu Ile
130 135 140
Tyr Ala Lys Leu Tyr Met Tyr Gln Ile Cys Arg Ser Leu Ala Tyr Ile
145 150 155 160
His Asn Ser Ile Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu
165 170 175
Leu Val Asn Pro His Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser
180 185 190
Ala Lys Val Leu Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser
195 200 205
Arg Tyr Tyr Arg Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr
210 215 220
Thr Ala Ile Asp Val Trp Ser Ala Gly Cys Val Leu Ala Glu Leu Leu
225 230 235 240
Leu Gly Gln Pro Ile Phe Pro Gly Asp Ser Gly Val Asp Gln Leu Val
245 250 255
Glu Ile Ile Lys Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Cys
260 265 270
Met Asn Pro Asn Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His
275 280 285
Pro Trp His Lys Ile Phe His Lys Arg Met Pro Ala Glu Ala Val Asp
290 295 300
Leu Val Ser Arg Leu Leu Gln Tyr Ser Pro Ser Leu Arg Ser Thr Ala
305 310 315 320
Leu Glu Ala Leu Ile His Pro Phe Phe Asp Glu Leu Arg Asp Pro Asn
325 330 335
Thr Arg Leu Pro Asn Gly Arg Phe Leu Pro Pro Leu Phe Asn Phe Lys
340 345 350
Pro His Glu Leu Lys Gly Val Pro Met Asp Ile Leu Val Lys Leu Ile
355 360 365
Pro Glu His Ala Arg Lys Asn Cys Ala Phe Val Gly Trp
370 375 380
<210>176
<211>1581
<212>DNA
＜213〉petunia (Petunia hybrida)
<400>176
tctctcaggt ttagggtttc gtattgtccg atcgctatac tttgaagctc tttccgatca 60
ctgttttgtg tttttgtaaa atgtgagctg ttctttagct acaggtttga agctgttaat 120
ctgtcatttg agttgtgccc attactcagt agtataagta agtttatttt tgttgctgat 180
atggcgtctg gtataatgcc ttcggctggt ggaaaacatc gaactgatgc catgcttgtt 240
gacaaacttc ccgaagaaat aaatgaaatg aagatcagag atgataaagc agaaaaggaa 300
atggaagcag ctgtagtgga tggaaatgga actgaaaaag gccacatcat cgtgacaact 360
attgggggca aaaatggtga gcctaagcag accattagtt acatggccga gcgtgttgtt 420
ggacagggtt cgtttggaat agtgttccag gccaaatgcc ttgaaactgg agaaactgtt 480
gcaataaaaa aggttttaca ggataagaga tacaagaatc gggaattgca aacaatacgc 540
cttctagatc atcctaatgt tgttgcactg aggcactgct tcttttcaac cacagaaaag 600
gatgagcttt atctgaattt ggtccttgaa tatgtaccag agactgtcta ccgtgtcttg 660
agacattaca gcaaagcaaa ccaacagatg cctatgattt atgtcaagct ctacacatat 720
cagattttca gagctttggc ctacatacac ggcataggag tctgccacag ggacatcaag 780
cctcagaatc tactggtcaa cccccacacc caccagctta agctctgcga ctttgggagt 840
gcaaaagttc tggtcaaagg cgaaccaaat atttcatata tttgttctcg ttactatcgt 900
gcacccgaac ttatattcgg agcaactgaa tacacttttg caattgacat ttggtctgtg 960
ggttgcgtcc ttgccgaact gcttctgggg cagcccctct ttcctggtga gagtggagtt 1020
gatcagcttg ttgaaataat caaggttctt ggaacaccaa ctcgggagga aatcaagagt 1080
atgaatccaa attacactga gttcaaattc ccacaaatca aagctcaccc ttggcacaaa 1140
atttttcata agcggatgcc tccagaagct gtggaccttg tgtcaaggct tctccaatat 1200
tctccaaatt tgaggtccac tgcgttggag gcttgcactc acactttctt tgatgaactc 1260
cgtgatccta agactcgcct ccctaatggt cggccattgc cacctctttt caacttcagg 1320
cctcaagagc tgaaaggagc gtcggcagac ctcttaaaca agctgatacc agaacatgct 1380
aagaagcagt gtacctttct tggtgtctag gtttatgaat gttgtgtatc tattgtatac 1440
ttgaaatatt ttgcaaccac tggttaattc tctactttgg ctaaacctgt ttgacatgtc 1500
ttcctttcca tgaaaactta taactgaaac cacattgtgt aagttgttcc tagtagtgca 1560
aatgctattt acttgaactt g 1581
<210>177
<211>409
<212>PRT
＜213〉petunia
<400>177
Met Ala Ser Gly Ile Met Pro Ser Ala Gly Gly Lys His Arg Thr Asp
1 5 10 15
Ala Met Leu Val Asp Lys Leu Pro Glu Glu Ile Asn Glu Met Lys Ile
20 25 30
Arg Asp Asp Lys Ala Glu Lys Glu Met Glu Ala Ala Val Val Asp Gly
35 40 45
Asn Gly Thr Glu Lys Gly His Ile Ile Val Thr Thr Ile Gly Gly Lys
50 55 60
Asn Gly Glu Pro Lys Gln Thr Ile Ser Tyr Met Ala Glu Arg Val Val
65 70 75 80
Gly Gln Gly Ser Phe Gly Ile Val Phe Gln Ala Lys Cys Leu Glu Thr
85 90 95
Gly Glu Thr Val Ala Ile Lys Lys Val Leu Gln Asp Lys Arg Tyr Lys
100 105 110
Asn Arg Glu Leu Gln Thr Ile Arg Leu Leu Asp His Pro Asn Val Val
115 120 125
Ala Leu Arg His Cys Phe Phe Ser Thr Thr Glu Lys Asp Glu Leu Tyr
130 135 140
Leu Asn Leu Val Leu Glu Tyr Val Pro Glu Thr Val Tyr Arg Val Leu
145 150 155 160
Arg His Tyr Ser Lys Ala Asn Gln Gln Met Pro Met Ile Tyr Val Lys
165 170 175
Leu Tyr Thr Tyr Gln Ile Phe Arg Ala Leu Ala Tyr Ile His Gly Ile
180 185 190
Gly Val Cys His Arg Asp Ile Lys Pro Gln Asn Leu Leu Val Asn Pro
195 200 205
His Thr His Gln Leu Lys Leu Cys Asp Phe Gly Ser Ala Lys Val Leu
210 215 220
Val Lys Gly Glu Pro Asn Ile Ser Tyr Ile Cys Ser Arg Tyr Tyr Arg
225 230 235 240
Ala Pro Glu Leu Ile Phe Gly Ala Thr Glu Tyr Thr Phe Ala Ile Asp
245 250 255
Ile Trp Ser Val Gly Cys Val Leu Ala Glu Leu Leu Leu Gly Gln Pro
260 265 270
Leu Phe Pro Gly Glu Ser Gly Val Asp Gln Leu Val Glu Ile Ile Lys
275 280 285
Val Leu Gly Thr Pro Thr Arg Glu Glu Ile Lys Ser Met Asn Pro Asn
290 295 300
Tyr Thr Glu Phe Lys Phe Pro Gln Ile Lys Ala His Pro Trp His Lys
305 310 315 320
Ile Phe His Lys Arg Met Pro Pro Glu Ala Val Asp Leu Val Ser Arg
325 330 335
Leu Leu Gln Tyr Ser Pro Asn Leu Arg Ser Thr Ala Leu Glu Ala Cys
340 345 350
Thr His Thr Phe Phe Asp Glu Leu Arg Asp Pro Lys Thr Arg Leu Pro
355 360 365
Asn Gly Arg Pro Leu Pro Pro Leu Phe Asn Phe Arg Pro Gln Glu Leu
370 375 380
Lys Gly Ala Ser Ala Asp Leu Leu Asn Lys Leu Ile Pro Glu His Ala
385 390 395 400
Lys Lys Gln Cys Thr Phe Leu Gly Val
405
<210>178
<211>2193
<212>DNA
＜213〉rice
<400>178
aatccgaaaa gtttctgcac cgttttcacc ccctaactaa caatataggg aacgtgtgct 60
aaatataaaa tgagacctta tatatgtagc gctgataact agaactatgc aagaaaaact 120
catccaccta ctttagtggc aatcgggcta aataaaaaag agtcgctaca ctagtttcgt 180
tttccttagt aattaagtgg gaaaatgaaa tcattattgc ttagaatata cgttcacatc 240
tctgtcatga agttaaatta ttcgaggtag ccataattgt catcaaactc ttcttgaata 300
aaaaaatctt tctagctgaa ctcaatgggt aaagagagag atttttttta aaaaaataga 360
atgaagatat tctgaacgta ttggcaaaga tttaaacata taattatata attttatagt 420
ttgtgcattc gtcatatcgc acatcattaa ggacatgtct tactccatcc caatttttat 480
ttagtaatta aagacaattg acttattttt attatttatc ttttttcgat tagatgcaag 540
gtacttacgc acacactttg tgctcatgtg catgtgtgag tgcacctcct caatacacgt 600
tcaactagca acacatctct aatatcactc gcctatttaa tacatttagg tagcaatatc 660
tgaattcaag cactccacca tcaccagacc acttttaata atatctaaaa tacaaaaaat 720
aattttacag aatagcatga aaagtatgaa acgaactatt taggtttttc acatacaaaa 780
aaaaaaagaa ttttgctcgt gcgcgagcgc caatctccca tattgggcac acaggcaaca 840
acagagtggc tgcccacaga acaacccaca aaaaacgatg atctaacgga ggacagcaag 900
tccgcaacaa ccttttaaca gcaggctttg cggccaggag agaggaggag aggcaaagaa 960
aaccaagcat cctcctcctc ccatctataa attcctcccc ccttttcccc tctctatata 1020
ggaggcatcc aagccaagaa gagggagagc accaaggaca cgcgactagc agaagccgag 1080
cgaccgcctt cttcgatcca tatcttccgg tcgagttctt ggtcgatctc ttccctcctc 1140
cacctcctcc tcacagggta tgtgcccttc ggttgttctt ggatttattg ttctaggttg 1200
tgtagtacgg gcgttgatgt taggaaaggg gatctgtatc tgtgatgatt cctgttcttg 1260
gatttgggat agaggggttc ttgatgttgc atgttatcgg ttcggtttga ttagtagtat 1320
ggttttcaat cgtctggaga gctctatgga aatgaaatgg tttagggtac ggaatcttgc 1380
gattttgtga gtaccttttg tttgaggtaa aatcagagca ccggtgattt tgcttggtgt 1440
aataaaagta cggttgtttg gtcctcgatt ctggtagtga tgcttctcga tttgacgaag 1500
ctatcctttg tttattccct attgaacaaa aataatccaa ctttgaagac ggtcccgttg 1560
atgagattga atgattgatt cttaagcctg tccaaaattt cgcagctggc ttgtttagat 1620
acagtagtcc ccatcacgaa attcatggaa acagttataa tcctcaggaa caggggattc 1680
cctgttcttc cgatttgctt tagtcccaga attttttttc ccaaatatct taaaaagtca 1740
ctttctggtt cagttcaatg aattgattgc tacaaataat gcttttatag cgttatccta 1800
gctgtagttc agttaatagg taatacccct atagtttagt caggagaaga acttatccga 1860
tttctgatct ccatttttaa ttatatgaaa tgaactgtag cataagcagt attcatttgg 1920
attatttttt ttattagctc tcaccccttc attattctga gctgaaagtc tggcatgaac 1980
tgtcctcaat tttgttttca aattcacatc gattatctat gcattatcct cttgtatcta 2040
cctgtagaag tttctttttg gttattcctt gactgcttga ttacagaaag aaatttatga 2100
agctgtaatc gggatagtta tactgcttgt tcttatgatt catttccttt gtgcagttct 2160
tggtgtagct tgccactttc accagcaaag ttc 2193
<210>179
<211>54
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm5797
<400>179
ggggacaagt ttgtacaaaa aagcaggctt aaacaatggg ttcagtaggg gttg 54
<210>180
<211>50
<212>DNA
＜213〉artificial sequence
<220>
＜223〉primer: prm5798
<400>180
ggggaccact ttgtacaaga aagctgggtg aagctgtctc atactcctgc 50
<210>181
<211>1243
<212>DNA
＜213〉rice
<400>181
aaaaccaccg agggacctga tctgcaccgg ttttgatagt tgagggaccc gttgtgtctg 60
gttttccgat cgagggacga aaatcggatt cggtgtaaag ttaagggacc tcagatgaac 120
ttattccgga gcatgattgg gaagggagga cataaggccc atgtcgcatg tgtttggacg 180
gtccagatct ccagatcact cagcaggatc ggccgcgttc gcgtagcacc cgcggtttga 240
ttcggcttcc cgcaaggcgg cggccggtgg ccgtgccgcc gtagcttccg ccggaagcga 300
gcacgccgcc gccgccgacc cggctctgcg tttgcaccgc cttgcacgcg atacatcggg 360
atagatagct actactctct ccgtttcaca atgtaaatca ttctactatt ttccacattc 420
atattgatgt taatgaatat agacatatat atctatttag attcattaac atcaatatga 480
atgtaggaaa tgctagaatg acttacattg tgaattgtga aatggacgaa gtacctacga 540
tggatggatg caggatcatg aaagaattaa tgcaagatcg tatctgccgc atgcaaaatc 600
ttactaattg cgctgcatat atgcatgaca gcctgcatgc gggcgtgtaa gcgtgttcat 660
ccattaggaa gtaaccttgt cattacttat accagtacta catactatat agtattgatt 720
tcatgagcaa atctacaaaa ctggaaagca ataaggaata cgggactgga aaagactcaa 780
cattaatcac caaatatttc gccttctcca gcagaatata tatctctcca tcttgatcac 840
tgtacacact gacagtgtac gcataaacgc agcagccagc ttaactgtcg tctcaccgtc 900
gcacactggc cttccatctc aggctagctt tctcagccac ccatcgtaca tgtcaactcg 960
gcgcgcgcac aggcacaaat tacgtacaaa acgcatgacc aaatcaaaac caccggagaa 1020
gaatcgctcc cgcgcgcggc ggcggcgcgc acgtacgaat gcacgcacgc acgcccaacc 1080
ccacgacacg atcgcgcgcg acgccggcga caccggccat ccacccgcgc cctcacctcg 1140
ccgactataa atacgtaggc atctgcttga tcttgtcatc catctcacca ccaaaaaaaa 1200
aggaaaaaaa aacaaaacac accaagccaa ataaaagcga caa 1243

Claims (31)

1. increase the method for plant yield with respect to corresponding wild-type plant, be included in the I class HDZip hox5 nucleic acid of introducing in the plant and expressing coding I class homeodomain leucine zipper HDZip hox5 polypeptide, randomly selection has the plant of the productive rate of increase, wherein said I class HDZip hox5 nucleic acid is the nucleic acid of coding SEQ ID NO:2
Wherein, described plant is cereal.
2. express according to the process of claim 1 wherein that described I class HDZip hox5 nucleic acid is crossed in plant, described plant is rice, corn, wheat, barley, chestnut, rye, oat or Chinese sorghum.
3. according to the process of claim 1 wherein that described I class HDZip hox5 nucleic acid effectively is connected in constitutive promoter.
4. according to the method for claim 3, wherein constitutive promoter is the GOS2 promotor.
5. according to the method for claim 4, wherein, constitutive promoter is rice GOS2 promotor.
6. according to the method for claim 5, wherein constitutive promoter is shown in SEQ ID NO:33 or SEQ ID NO:178.
7. according to the process of claim 1 wherein that the productive rate of described increase is selected from following one or more aspect: each of the full seed number of increase, the seed overall yield of increase, increase be paniculiform spend the root diameter of the harvest index (HI) of number, the full rate of seed that increases, increase, the thousand seed weight (TKW) that increases, the root length that increases or increase, wherein each aspect for corresponding wild-type plant.
8. according to the process of claim 1 wherein that the productive rate of described increase produces under the abiotic stress condition.
9. method according to Claim 8, wherein said abiotic stress is that osmotic stress, oxidative stress or ion are coerced.
10. according to the method for claim 9, wherein said osmotic stress is selected from: water is coerced and salt stress.
11. according to the method for claim 10, it is drought stress that wherein said water is coerced.
12. method according to Claim 8 wherein shows as the productive rate of the increase that is selected from following one or more aspects for the tolerance of described abiotic stress: each of the full seed number of increase, the seed overall yield of increase, increase be paniculiform spends the root diameter of the root length of TKW, increase of HI, the increase of number, the full rate of seed that increases, increase or increase, wherein each aspect is with respect to corresponding wild-type plant.
13. produce the method for transgenic plant, plant part or vegetable cell, it comprises that wherein, described plant is cereal by obtaining this plant, plant part or vegetable cell according to each method in the claim 1 to 12.
14. following construct is according to the purposes in each the method in the claim 1 to 12, described construct comprises:
(i) defined I class HDZip hox5 nucleic acid in the claim 1;
(ii) can drive one or more control sequences that (i) amplifying nucleic acid sequence is expressed; Randomly
(iii) transcription termination sequence.
15. according to the purposes of the construct of claim 14, wherein said control sequence is constitutive promoter.
16. according to the purposes of the construct of claim 15, wherein said constitutive promoter is the GOS2 promotor.
17. according to the purposes of claim 16, wherein, constitutive promoter is rice GOS2 promotor.
18. according to the purposes of claim 17, wherein, constitutive promoter is shown in SEQ ID NO:33 or SEQ ID NO:178.
19. produce the method for genetically modified plant, plant part or vegetable cell, comprise with construct and transform plant, plant part or vegetable cell, wherein said construct comprises defined I class HDZip hox5 nucleic acid in the claim 1 that is under the control of GOS2 promotor, wherein, described plant is cereal.
20. produce the method for transgenic plant that has the productive rate of increase with respect to corresponding wild-type plant, described method comprises:
(i) in plant or vegetable cell, introduce and express defined I class HDZip hox5 nucleic acid in the claim 1;
(ii) culturing plants cell under the condition that promotes plant-growth and growth,
Wherein, described plant is cereal.
21. according to the method for claim 20, the productive rate of wherein said increase takes place under the abiotic stress condition that increases.
22. according to claim 13,19 or 20 method, wherein said plant is cereal.
23. according to the method for claim 22, wherein said plant is rice, corn, wheat, barley, chestnut, rye, oat or Chinese sorghum.
24. according to claim 13,19 or 20 method, comprise the part gathered in the crops that obtains described transgenic plant.
25. according to the method for claim 24, wherein said gather in the crops the part be seed.
26. defined I class HDZip hox5 nucleic acid is in the purposes that increases with respect to corresponding wild-type plant in the plant yield in the claim 1, wherein, described plant is cereal.
27. according to the purposes of claim 26, the productive rate of wherein said increase is selected from following one or more aspect: each of the full seed number of increase, the seed overall yield of increase, increase be paniculiform spend the root diameter of the harvest index (HI) of number, the full rate of seed that increases, increase, the thousand seed weight (TKW) that increases, the root length that increases or increase, wherein each aspect for corresponding wild-type plant.
28. according to the purposes of claim 26 or 27, the productive rate of wherein said increase takes place under the abiotic stress condition that increases.
29. defined I class HDZip hox5 nucleic acid be used for to select to have the purposes of plant of the growth characteristics of improvement in the claim 1 in the procedure of breeding as molecule marker, wherein, described plant is cereal.
30. claim 1,13,19 or 20 method, wherein said I class HDZip hox5 nucleic acid is the represented nucleic acid of SEQ ID NO:1.
31. claim 14,26 or 29 purposes, wherein said I class HDZip hox5 nucleic acid is the represented nucleic acid of SEQ ID NO:1.
CN200680049144XA 2005-11-07 2006-11-07 Plants having improved growth characteristics and a method for making the same Expired - Fee Related CN101351556B (en)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
EP05110429 2005-11-07
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CN103667310A (en) * 2013-02-06 2014-03-26 中国农业科学院作物科学研究所 Arabidopsis thaliana nitrate transporter gene NRT3.1, and encoding protein and application thereof
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CN108610405B (en) * 2018-05-16 2020-11-03 中国科学院遗传与发育生物学研究所 Application of protein TaNRT2.5 in regulation and control of plant root system development
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