CN101347126A - Sterilizing and virus-eradicating composition with improved sterilizing capability - Google Patents
Sterilizing and virus-eradicating composition with improved sterilizing capability Download PDFInfo
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- CN101347126A CN101347126A CNA2007101816125A CN200710181612A CN101347126A CN 101347126 A CN101347126 A CN 101347126A CN A2007101816125 A CNA2007101816125 A CN A2007101816125A CN 200710181612 A CN200710181612 A CN 200710181612A CN 101347126 A CN101347126 A CN 101347126A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
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Abstract
The invention relates to a bacterium and virus killing combination with improved killing performance, especially a bacterium and virus killing combination with improved killing performance comprising Potassium Monopersulfate Compound, sodium hexametaphosphate, malic acid, citric acid and tartaric acid. The combination in the invention is made from natural substances, can be biodegraded by microorganism, produces almost no environmental pollution, achieves better disinfection effect than the previously widely used VIRCON-S in extensive bacterium and virus killing under the condition of organic substance bulk processing, consequently, is suitable for the disinfection of not only barns but also carcasses and drinking water.
Description
Technical field
The present invention relates to sterilization and virucidal compositions that a kind of disinfecting power is improved, further relate to a kind of at Potassium Monopersulfate compound (potassium monopersulfate) thus and comprise sterilization and the virucidal compositions that citric acid and tartaric acid disinfecting power improve in the malic acid.
Background technology
In recent years, the pathogene that becomes problem in animal husbandry had the birds influenza virus, aphthovirus, hog cholera virus, salmonella, streptococcus etc.
Especially, the main birds influenza virus of bringing harm for poultries such as chicken, turkey, can be divided into high pathogenicity, weak pathogenicity, avirulence 3 classes according to pathogenicity, international epizootic disease affairs offices (OIE) classify high pathogenicity wherein as the A grade, classify the 1st class livestock contagious disease as in Korea S.Pathogene is an A type virus, has H serotype and N serotype.Can there be totally 135 kinds virus serotype in protein according to this two class, but till the report 15 kinds of HA, 9 kinds of NA is arranged in the whole world now by 2004.Infecting mainly is to take place when direct contact birds secretion, pin, feed carriage, utensil, equipment that can also be by the spittle, water, people, sticks in the propagation such as ight soil on the eggshell.Though symptom is different according to the pathogenicity of infected virus, the rapid minimizing of respiratory apparatus symptom and diarrhea, laying rate can appear in major part.According to circumstances, cyan disease occurs, the facial phenomenon that edema takes place or become mildewed and assemble also occurs to a side at heads such as cockscombs.
According to pathogenicity, lethality between 0～100%, but because and symptoms such as ewcastle disease, infectious laryngotracheitis, mycoplasma infection disease similar, therefore need correct diagnosis.
Though do not take place in the world later in nineteen thirty generation, nineteen eighty-three since European countries such as Belgium, France begin, taken place all in countries in the world by 2004 to comprise that the high pathogenicity birds of weak pathogenicity are malicious feels.Under the pathogenic situation of height, the mankind also can be infected, thus 6 people's death in Hong Kong in 1997, and 2004 in Vietnam's 16 people's death.Also the sense of birds poison taking place and be diffused into the whole nation in the cloudy city of North Chungchong December from 1996 to 2003 in Korea S, but owing to be weak pathogenicity, therefore do not confirm as and infect on the human body.
If the sense of birds poison takes place, then the most of country in the whole world will carry out the processing of slaughtering of entire quantity, and generation country can not export the product of raising chickens.
Therefore, we can say that the birds influenza virus is the arch-criminal of harm animal husbandry development.
Aftosa is to take place when domestic animal is infected by aphthovirus, also once occurs in Korea S, Japan in 2000, occurs in since calendar year 2001 in the worldwide such as Britain.The main area that takes place is Africa, South America, the Asia, Eastern Europe etc., August 30 calendar year 2001, the non-generation of existing OIE approval was state-owned, the U.S., Canada, northern Sino-U.S. country such as Panama and Norway (West Europe), Australia, New Zealand (Oceania) and Indonesia, more than 50 countries such as Japan (Asia), zonal approved Colombia northwest (NW) portion that has, Filipine Mindanao area etc., the country that implements vaccine has, Paraguay and Brazil, Colombian some areas, with regard to the situation of Korea S, originally only approved area, Ji Zhou island, and in September 19 calendar year 2001 and France, Holland, Ireland etc. together are recognized as the non-generation state (clear disease state) that implements vaccine, thereby have recovered original status.
Above-mentioned aftosa can make the yielding ability of animal low, cause economic loss, so classify aftosa as in the international trade restriction object disease, be classified as in 15 kinds of diseases of A level in international epizootic disease affairs offices (OIE), also as first pernicious infectious disease of bending, in case the generation aftosa then is difficult to radical cure, does not also have special methods of treatment.
For Developing of Animal Industry, the needs exploitation is a kind of not only to have high elimination power to the above-mentioned pathogene of enumerating but also to pathogen and the virus that becomes various diseases, and the wide disinfectant of safety, the scope of application that can freely carry out disinfection to carcass, drinking-water etc.
Natural goods means crude former nature, and the usage amount or the suitable food of natural goods that is classified as GRAS (GenerallyRecognized As Safe) is unrestricted, and domestic in Korea S is that it is classified as natural additive, uses as food additives.
Country beyond the Korea S, the material that natural goods can freely utilize as there is no particular limitation and according to user's intention, its functional excellence, thereby be applied to healthy food and pharmaceuticals.
Malic acid is as being rich in material in natural fruit, by U.S. food medicine State Security Department (FDA) authentication for the material of safety in general (generally recognized as safe, GRAS).Malic acid also is registered the pharmaceuticals bulletin material into the food medicine Room, and the raw material that therefore also can be used as pharmaceuticals uses, and uses for a long time as additive in cold drink, fruit and vegetable tin, fruit juice, sugar/chewing gum etc.
At present, very big effort has been paid in the whole world in order to utilize natural materials to develop disinfectant, in commercially available similar disinfectant, has the VIRCON-S as the Bayer Korea of main material such as malic acid, Potassium Monopersulfate compound as representative.
Above-mentioned VIRCON-S is a disinfectant best-selling as the aftosa disinfectant, that pirbright research institute identifies, be considered to not only virus, bacterium, various pathogene such as mould are demonstrated remarkable extinction effects, and young dog, day old chick, sucking pig, cow etc. needed the disinfectant of the safety that the follower of safety also can directly spray.In the above-mentioned disinfectant of every 1kg, contain Potassium Monopersulfate compound 500g, sodium chloride 15g, malic acid 100g, and when sterilization re-uses after diluting with 50～300 times water.
But,, during situation that the repellence of pathogene strengthens in time, just should continue exploitation and development natural materials when considering because of numerous factors such as the long-term use of chemicals and environmental pollutions.Thereby in the art, the needs exploitation is a kind of not only to be compared with the commercially available disinfectant of the pathogen that can eliminate various diseases and virus, has the sterilization of high sterilizing ability and cancha, and can freely carry out the natural disinfectant agent of carcass, disinfection of drinking water etc.
Therefore, present inventors etc. have the natural disinfectant agent of high sterilizing ability and carcass disinfecting power and found that of concentrating on studies in order to prepare for various pathogene, in malic acid and Potassium Monopersulfate compound, contain citric acid and tartaric composition with specific ratios, compare with at present commercially available VIRCON-S, its disinfecting power obviously improves and keeps stability, so that finished the present invention.
Summary of the invention
The objective of the invention is to, provide a kind of and in malic acid and Potassium Monopersulfate compound, contain citric acid and tartaric sterilization and virucidal compositions with specific ratios.
For achieving the above object, the invention provides and comprise sterilization and the virucidal compositions that Potassium Monopersulfate compound, calgon, malic acid, citric acid and tartaric disinfecting power are improved.
Below, explain the present invention.
With regard to sterilization of the present invention and virucidal compositions, preferred above-mentioned composition comprises: Potassium Monopersulfate compound (potassium monopersulfate) 30～50 weight portions, calgon 1～20 weight portion, malic acid 1～10 weight portion, citric acid 1～10 weight portion, tartaric acid 1～10 weight portion.
Potassium Monopersulfate compound (potassiummonopersulfate) as one of component required in this invention closes salt as three of potassium peroxymonosulfate, potassium sufate and potassium bisulfate, be as trade name "
Jade hands " material that is widely known by the people, its main component as disinfectant is used, and soluble in water, is the chlorine-free oxidant.With chlorine is that oxidant is compared, and does not have penetrating odor, and demonstrates very strong Disinfection Effect by strong oxidation.
The content of preferred above-mentioned Potassium Monopersulfate compound is 30～50 weight portions.If the content of above-mentioned Potassium Monopersulfate compound is lower than 30 weight portions, then there is the faint problem of sterilization and disinfecting power, if be higher than 50 weight portions, then exist to cross by force and bring the problem of harm for human body or domestic animal because of toxicity.
As the calgon of one of neccessary composition of the present invention, have soluble in waterly, and hydrolyzable is an orthophosphates in high-temperature water solution, in polymeric phosphate, the unique demonstration of its aqueous solution is neutral.Known its is generally used for keeping in the stabilizing agent of correlated products such as the moisture capacity of sausage, the variable color that prevents fruit juice, pH buffer, the anti-coagulating agent of pectin, protein dispersant and vegetables, fruit, beans processed goods, also can suppress the microbial growth that is caused by the metal ion inactivation.Also have, when main poultry cancha or livestock products utensil and peripheral facility thereof are metal, play prevention or eliminate the effect of metal erosion, and the surface of protection irony body and play the effect of the corrosion of prevention irony body.
In the present invention, the content of preferred above-mentioned calgon is 1～20 weight portion.If the content of above-mentioned calgon is lower than 1 weight portion, then there is prevention and eliminates the faint problem of effect that the irony body corrodes, if be higher than 20 weight portions, then there is the problem that hinders the effect that suppresses corrosion of metal.
One of neccessary composition of the present invention malic acid, be by acid its sterilization and kill the virus effect by people widely material, U.S. food medicine State Security Department (FDA) authenticates that (Generally RecognizedAs Safe is common safety GRAS), is also referred to as maleic acid (maleic acid).The chemical formula of malic acid (DL-Maleic acid) is C
4H
6O
5, molecular weight 134.09, proportion 1.601,133 ℃ of fusing points, 150 ℃ of boiling points (decomposition).Malic acid does not have hygroscopy, and its tart flavour degree is stronger approximately by 20% than citric acid, and is rich in natural fruits such as apple and grape.If acid malic acid is put in the microorganism, then microorganism draws in organic acid in the microorganism in order to keep pH raising property.Because of This move, cells of microorganisms matter becomes acidification, and enzymatic reaction and material Transfer process are brought influence, also be raising pH consumed energy, thereby microorganism is eliminated.Also have, malic acid has the smell of mitigation disinfection sanitizer and the effect of stimulation.
In the present invention, the content of preferred above-mentioned malic acid is 1～10 weight portion.If the content of malic acid is lower than 1 weight portion, then there is the faint problem of abirritation, if be higher than 10 weight portions, then there is the problem of the drug effect decline of sterilization on the contrary and disinfectant.
Citric acid is as one of necessary component of the present invention, is the acid that contains with free state acid in the nuclear of numerous plants or fruit juice, and it mainly is extensive use of with multiple uses such as the fragrant preservative agent of fruit, keeping quality synergist, pH regulator agent.Also have, in the winestone that tartaric acid is contained in when making grape wine to be precipitated, be widely used in syrup, fruit juice etc., be used for the mixing of preparation, buffer, pH regulator agent etc. as pharmaceuticals as cold drink, but not as the report of disinfectant applications.These are included the safe material in safe food additives (GRSA) catalogue of being considered to usually of assert of FDA, they play the effect of preservative and have the effect that prevents the corruption that caused by various organic matters, and the stronger drug effect of performance when together permeating with sterilization and disinfectant composition.Thereby, by these are added in the Potassium Monopersulfate compound with appropriate amount, play the effect of promoting the disinfecting power of Potassium Monopersulfate compound by acidity.
In the present invention, preferred above-mentioned citric acid and tartaric content are respectively 1～10 weight portion.If citric acid and tartaric content are lower than 1～10 weight portion, then almost do not have Disinfection Effect and prevent corrupt effect, if be higher than 10 weight portions, then there is the problem of the drug effect decline of sterilization and disinfectant.
In sterilization of the present invention and virucidal compositions, for the various compositions of sterilization and disinfectant equably emulsification be scattered in the above-mentioned composition, can add dodecyl sodium sulfate again, at this moment, preferred above-mentioned dodecyl sodium sulfate content is 1～35 weight portion.
In sterilization of the present invention and virucidal compositions,, can further add pigment, ester etc. to above-mentioned composition in order to improve look perfume (or spice).
Sterilization of the present invention and virucidal compositions, by in Potassium Monopersulfate compound (potasiummonopersulfate), calgon and malic acid, adding citric acid and tartaric acid, with by calgon, neopelex, malic acid, sodium chloride and sulfamic acid (sulfamic acid) sterilizing ability that constitute, present widely used disinfectant VIRCON-S compare, demonstrating has increased about 2～4 times sterilizing ability (with reference to table 1～table 9).Thereby, because sterilization of the present invention and virucidal compositions are prepared from by natural materials, so can carry out the biological degradation of microorganism, therefore can cause environmental pollution hardly, and carrying out under a large amount of conditions of handling of organic matter, yet, therefore be not only applicable to the cancha sterilization, but also go for carcass, disinfection of drinking water etc. for the bacterium of wide range and the Disinfection Effect excellence of virus.
Sterilization of the present invention and virucidal compositions, bacteriums such as salmonella, brucella, colon bacillus, Pasteurella, staphylococcus can be eliminated, viruses such as Avian pneumo-encephalitis virus, birds influenza virus, pig aujesky's disease poison, hog cholera virus, aphthovirus can be eliminated.
Embodiment
Below, further describe the present invention by embodiment.The foregoing description is just for illustration the present invention, and scope of the present invention is not limited to embodiment.
＜embodiment 1〉preparation 1 of bactericidal composition
With Potassium Monopersulfate compound 500g, malic acid 100g, citric acid 15g, tartaric acid 10g, calgon 50g and neopelex 319g, a small amount of haematochrome and lemonene powder, powder method for making based in the Da Han official preparation general provisions has prepared disinfectant.
＜embodiment 2〉preparation 2 of bactericidal composition
Except Potassium Monopersulfate compound 500g, malic acid 100g, citric acid 100g, tartaric acid 50g, calgon 125g and neopelex 25g, the method by has similarly to Example 1 prepared disinfectant.
＜experimental example 1〉bactericidal effect
＜1-1〉measure the bactericidal effect of salmonella
From the Korea S microorganism preserve center (KCCM) obtain salmonella as the representative strain of common bacteria (Salmonella cholearsuis, KCCM41598) and be used for test.
Utilize and to have carried out autoclaved nutrient medium, test strain after carrying out 22～26 hours successive transfer culture under 37 ℃, is measured turbidity according to the McFaland method and measured the bacterium number, and to have used the concentration of bacterium be 10
8The sample that CFU/mL is above.
The composition of the foregoing description 1 is divided into treatment region 1, treatment region 2, treatment region 3, treatment region 4, treatment region 5, and treatment region 1 is made as distilled water condition (organic matter: do not have), treatment region 2 is made as hard water condition (organic matter: low concentration), treatment region 3 is made as organic matter/hard water condition (organic matter: high concentration), treatment region 4 is made as the check plot of treatment region 2 and 3, treatment region 5 is made as the check plot of treatment region 1, and utilizes distilled water, hard water, 5% organic matter dilution to dilute accordingly with each treatment region.At this moment, dilute with 1/100,1/150,1/200,1/250,1/300,1/350,1/400,1/800,1/900,1/1000,1/1100,1/1200 and 1/1400 extension rate.
With regard to hard water, with CaCl
20.305g and MgCl
26H
2After O 0.139g is dissolved in distilled water 1L, carry out autoclaving, take care of again, with regard to the organic matter dilution, be dissolved in hard water, take care of in 4 ℃ again so that after containing 20% (w/v) yeast extract, carry out autoclaving with regard to bacterium in 4 ℃.In dilution disinfectant and dilution during bacterium, dilute modulation with distilled water, hard water, 5% organic matter respectively after, utilize the NaOH of 1N and the HCl adjusting pH of 1N, making pH is 7., with regard to the organic matter dilution, re-use after being dissolved in sterilized hard water, with regard to virus so that contain 5% hyclone.
Next, will be on 37 ℃ of cultivate down 4ml bacteriums, mix with distilled water, hard water, the 5% organic matter dilution of 96ml accordingly with treatment region, get the 2.5ml mixed liquor afterwards, be added drop-wise in 4 ℃ the disinfectant dilution using distilled water, hard water, 5% organic matter diluted respectively, and mixed with 1: 1.The reaction of disinfectant and pathogene is to carry out with 1 minute interval in order at each test tube, and 4 ℃ of following accurate responses 30 minutes, mixes once in per 10 minutes, so that react fully.
Behind the accurate response 30 minutes, for in and the usefulness of disinfectant, take out 1.0ml immediately, join 37 ℃, 9.0ml in and mix in the medium, afterwards according to the extension rate of each disinfectant, respectively add 0.1ml to 5 test tubes that nutrient medium is housed and mix, in 37 ℃ of incubators, cultivated 48 hours afterwards.Be used for and the disinfectant composition in and medium used the medium that in nutrient medium, contains 5% deactivation horse serum.
For each disinfectant extension rate, experimentize and measured the found nutrient medium number of bacterium at 5 nutrient mediums respectively.
With regard to bacterial reproduction whether judgement, in the nutrient medium of above-mentioned 5 identical disinfectant extension rates, be identified as more than 2 breeding takes place final sterilization dilution stage as extension rate.
Measure the bactericidal effect of composition of the present invention and be illustrated in the table 1 by said method.
[table 1]
Bactericidal effect for salmonella
As shown in table 1, as seen composition of the present invention is for salmonella, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1100, has bactericidal effect always, when organic substrate concentration is high (treatment region 3), extension rate up to 1/200 has bactericidal effect always.
＜1-2〉measure staphylococcic bactericidal effect
In order to estimate disinfection effect for specific bacteria, obtain from Korea S microorganism preservation center staphylococcus (Staphylococcus aureus, KCCM40050) and be used for the test.
With＜1-1〉same method tests, and its result is illustrated in the table 2.
[table 2]
For staphylococcic bactericidal effect
As shown in table 2, as seen composition of the present invention is for staphylococcus, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1200, has bactericidal effect always, when organic substrate concentration is high (treatment region 3), extension rate up to 1/250 has bactericidal effect always.
＜1-3〉measure the bactericidal effect of colon bacillus
In order to estimate disinfection effect for specific bacteria, obtain from Korea S microorganism preservation center colon bacillus (Escherichia coli, KCCM11234) and be used for the test.
With＜1-1〉same method tests, and its result is illustrated in the table 3.
[table 3]
Bactericidal effect for colon bacillus
As shown in table 3, as seen composition of the present invention is for colon bacillus, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1000, has bactericidal effect always, when concentration is high (treatment region 3), extension rate up to 1/150 has bactericidal effect always.
＜1-4〉measure the bactericidal effect of Pasteurella
In order to estimate disinfection effect, obtain Pasteurella (Pasteurella multocida type D4) and be used for test from state-run veterinary science quarantine institute for specific bacteria.
Pasteurella is inoculated into carries out the autoclaved medium of making by the immersion liquid of brain and heart (brain hear infusion broth, BHI broth) in, and under 37 ℃, carry out 22～26 hours successive transfer culture, measure turbidity according to methods such as McFaland afterwards and measure the bacterium number, and to have used the concentration of bacterium be 10
8Bacterium on the medium more than the CFU/mL.
In test method, reaction finish the back in and the usefulness of disinfectant, take out 1.0ml immediately, join 37 ℃, 9.0ml in and mix in the medium, afterwards according to the extension rate of each disinfectant, respectively adding 0.1ml in 5 test tubes that BHI broth is housed mixes, in 37 ℃ of incubators, cultivated 48 hours afterwards, except be used for and the disinfectant composition in and medium used the medium that in BHI broth, contains 5% deactivation horse serum, with＜1-1〉same method tests, and its result is illustrated in the table 4.
[table 4]
Bactericidal effect for Pasteurella
As shown in table 4, as seen composition of the present invention is for Pasteurella, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1100, has bactericidal effect always, when organic substrate concentration is high (treatment region 3), extension rate up to 1/250 has bactericidal effect always.
＜1-5〉measure brucellar bactericidal effect
In order to estimate disinfection effect for specific bacteria, obtain brucella (Brucella ovis ATCC25840) and use from state-run veterinary science quarantine institute, with inoculation to carrying out in the autoclaved cloth Lu Shi medium (5%bovine serum contained brucellabroth) that contains 5% cow's serum, afterwards at 37 ℃, 5%CO
2Carry out 72 hours successive transfer culture in the incubator, measure turbidity according to methods such as McFaland afterwards and measured the bacterium number, and be 10 the concentration of bacterium
8Being used for more than the CFU/mL tests.
In test method, reaction finish the back in and the usefulness of disinfectant, take out 1.0ml immediately, join 37 ℃, 9.0ml in and mix in the medium, afterwards according to the extension rate of each disinfectant, respectively add 0.1ml to 5 test tubes that cloth Lu Shi medium is housed and mix, afterwards at 37 ℃, 5%CO
2Cultivated in the incubator 72 hours, except be used for and the disinfectant composition in and medium used in cloth Lu Shi medium and contained the medium of 5% deactivation horse serum, with＜1-1〉same method tests, and its result is illustrated in the table 5.
[table 5]
About brucellar bactericidal effect
As shown in table 5, as seen composition of the present invention is for brucella, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1000, has bactericidal effect always, when concentration is high (treatment region 3), extension rate up to 1/200 has bactericidal effect always.
＜experimental example 2〉effect of killing the virus
＜2-1〉measure the effect of killing the virus of birds influenza virus
In order to estimate the disinfection effect of disinfectant, used the viral H9N2 (MS96 strain) that obtains from state-run veterinary science quarantine institute for virus.
Strain is inoculated in the allantois of SPF embryonated egg of 10 ages in days, carries out successive transfer culture.Time point (virus titer 10 in the vigor maximum of the virus of having bred
7.0More than/the mL) adopt virus and be used in test.
The composition of the foregoing description 1 is divided into treatment region 1, treatment region 2, treatment region 3, prepare distilled water condition (organic matter: do not have) at treatment region 1, prepare hard water condition (organic matter: low concentration) at treatment region 2, prepare organic matter/hard water conditions (organic matter: high concentration), and utilize distilled water, hard water, 5% organic matter dilution to dilute accordingly at treatment region 3 with each treatment region.At this moment, dilute with 1/200,1/400,1/600,1/800,1/1000,1/1200,1/1400,1/1600,1/1800 and 1/2000 extension rate.
Next, the allantoic fluid 1.0ml that will contain virus, mix with distilled water, hard water and the 5% organic matter dilution (5% hyclone) of 24ml accordingly with treatment region, got 2.5ml afterwards every one minute, join in the test tube that 4 ℃ the disinfectant dilution that dilutes with distilled water, hard water, 5% organic matter dilution is housed and mix, 4 ℃ of following accurate responses 30 minutes, mixed once every 10 minutes, afterwards so that react fully.After the reaction of disinfectant finishes, in and the usefulness of disinfectant, immediately 50% non-assimilation hyclone of the thimerosal of 1 deal and 1 deal is mixed and makes its neutralization.Next, utilizing PBS is 10 with above-mentioned neutralizer as stoste and dilution
-1, 10
-2, 10
-3, 10
-4And 10
-5, for each disinfectant extension rate, neutralizer 0.2ml is inoculated in the allantois of 5 10 age in days SPF embryonated eggs, and in 37 ℃ incubator box, cultivated 5 days.Carry out ovoscopy every day, the embryonated egg of death within 24 hours after inoculating is removed from the test achievement as death by accident.By the red cell agglutination final decision viral existence whether reach virus titer.Utilize the Karber method of following mathematical expression 1 to calculate viral level.
[mathematical expression]
(L at this moment,
1Be the Log value of the minimum dilution measured,
L is the poor of Log value between the dilution,
S is the natural death % sum of virus in each dilution.)
Repeated 3 tests, measurement result is illustrated in the table 6.
[table 6]
The effect of killing the virus for the birds influenza virus
As shown in table 6, as seen composition of the present invention is for the birds influenza virus, (treatment region 1 or treatment region 2) extension rate when not having organic matter or concentration to hang down up to 1/1000, has the effect of killing the virus always, when concentration is high (treatment region 3), extension rate up to 1/400 has the effect of killing the virus always.
＜2-2〉measure the effect of killing the virus of Avian pneumo-encephalitis virus
In order to estimate disinfection effect, obtain Avian pneumo-encephalitis virus (Lasota strain) and be used for test from state-run veterinary science quarantine institute for virus.
Except reacting the back dilution is 10
-1, 10
-2, 10
-3, 10
-4, 10
-5And 10
-6In addition, with＜2-1〉same method tests, and its result is illustrated in the table 7.
[table 7]
The effect of killing the virus for Avian pneumo-encephalitis virus
As shown in table 7, as seen composition of the present invention is for Avian pneumo-encephalitis virus, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1000, has the effect of killing the virus always, when concentration is high (treatment region 3), extension rate up to 1/400 has the effect of killing the virus always.
＜2-3〉measure the effect of killing the virus of pig aujesky's disease poison
In order to estimate disinfection effect, obtain pig aujesky's disease poison (Yangsan strain) and be used for test from state-run veterinary science quarantine institute for virus.
Used successive transfer culture in the PK-15 cell line and great-hearted pig aujesky's disease poison, the content of virus when test is empty in advance, and be 10 with content
7.0Virus in the medium more than the/ml is used for test, and PK-15 cell culture medium is used in the reaction back, and it is 10 that neutralizer is diluted
-1, 10
-2, 10
-3, 10
-4, 10
-5And 10
-6For each extension rate, the reactant liquor 100 μ l that neutralized are inoculated in 5 wells on the tissue culture plate that 96 wells (well) are arranged that forms the PK-15 cell monolayer, cultivated 5 days down at 37 ℃ the inoculation back, whether the formation of usefulness microscopic examination every day CPE, except the whether existence of final decision virus, with＜2-1〉same method tests, and its result is illustrated in the table 8.
[table 8]
The effect of killing the virus for pig aujesky's disease poison
As shown in table 8, as seen composition of the present invention is for pig aujesky's disease poison, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1000, has the effect of killing the virus always, when concentration is high (treatment region 3), extension rate up to 1/400 has the effect of killing the virus always.
＜2-4〉measure the effect of killing the virus of hog cholera virus
In order to estimate disinfection effect, obtain hog cholera virus (LOM strain) and be used for test from state-run veterinary science quarantine institute for virus.
Used successive transfer culture in the PK-15 cell line and great-hearted hog cholera virus, the content of virus when test is empty in advance, and be 10 with content
7.0Virus in the medium more than the/ml is used for test, with viral liquid 1.0ml, as＜2-1 〉, mix with distilled water, hard water and the organic matter dilution (5% hyclone) of 19ml accordingly with each treatment region, got 2.5ml afterwards every one minute, join accordingly to be equipped with each treatment region and carried out in the test tube of 4 ℃ disinfectant dilution of dilution and mix with distilled water, hard water, 5% organic matter dilution, afterwards 4 ℃ of following accurate responses 30 minutes, mixed once every 10 minutes, so that react fully.With regard to control group, replace thimerosal to use distilled water.Next, if reaction finishes, then in and the usefulness of disinfectant, add reactant liquor and mixing to 37 ℃ neutralization with solution (cell culture fluid that contains 10%FBS) immediately with amount.
It is 10 that neutralizer is utilized the cell culture fluid dilution
-1, 10
-2, 10
-3, 10
-4, 10
-5And 10
-6After, for each extension rate, the solution 50 μ l that diluted are inoculated on 5 tissue culture plate that 96 wells (well) are arranged that formed the PK-15 cell monolayer, at 37 ℃ CO
2Cultivated 5 days in the incubator.Remove fixed cell behind the culture fluid, whether exist with regard to virus and to reach with regard to the virus titer, utilize the CSFV E2 monoclone antibody (monoclonal antibody) that engages (conjugate) FITC, whether the existence of evaluation hog cholera antigen, and finally judge.
The test triplicate carries out, and its result is illustrated in the table 9.
[table 9]
The effect of killing the virus for hog cholera virus
As shown in table 9, as seen composition of the present invention is for hog cholera virus, (treatment region 1 or treatment region 2) extension rate when not having organic matter or organic concentration to hang down up to 1/1000, has the effect of killing the virus always, when concentration is high (treatment region 3), extension rate up to 1/400 has the effect of killing the virus always.
Thereby, sterilization of the present invention and virucidal compositions, because when sterilization, if the organic matter that exists is many, then sterilize with 400 times extension rate, if organic matter is few, then can dilute is to re-use more than 1000 times, therefore with widely compare as the disinfecting power (50～300 times extension rates) of the VIRCON-S that has the disinfectant use now, demonstrate the more excellent sterilization and the effect of killing the virus, thereby be applicable to that various animals prevent disinfectant etc. with disinfectant, cancha disinfectant, aftosa, the sense of birds poison.
As described above, because sterilization of the present invention and virucidal compositions are made by natural goods, so can carry out the biological degradation of microorganism, therefore can cause environmental pollution hardly, and carrying out under a large amount of conditions of handling of organic matter, also compared with widely used VIRCON-S in the past, excellent more for the Disinfection Effect of bacterium and virus widely, thereby not only go for the cancha sterilization, but also go in carcass, the disinfection of drinking water etc.
Claims (6)
1. a sterilization and the virucidal compositions that disinfecting power is improved wherein comprises Potassium Monopersulfate compound, calgon, malic acid, citric acid and tartaric acid.
2. sterilization and virucidal compositions that disinfecting power according to claim 1 is improved is characterized in that,
Above-mentioned composition comprises Potassium Monopersulfate compound 30～50 weight portions, calgon 1～20 weight portion, malic acid 1～10 weight portion, citric acid 1～10 weight portion, tartaric acid 1～10 weight portion.
3. sterilization and virucidal compositions that disinfecting power according to claim 1 is improved is characterized in that,
Also comprise dodecyl sodium sulfate.
4. sterilization and virucidal compositions that disinfecting power according to claim 3 is improved is characterized in that,
The content of above-mentioned dodecyl sodium sulfate is 1～35 weight portion.
5. sterilization and virucidal compositions that disinfecting power according to claim 1 is improved is characterized in that,
Above-mentioned bacterium is any one that select from salmonella, brucella, colon bacillus, Pasteurella and staphylococcus.
6. sterilization and virucidal compositions that disinfecting power according to claim 1 is improved is characterized in that,
Above-mentioned virus is select from birds influenza virus, Avian pneumo-encephalitis virus, pig aujesky's disease poison and hog cholera virus, aphthovirus at least a.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020070072722 | 2007-07-20 | ||
| KR1020070072722A KR20090009442A (en) | 2007-07-20 | 2007-07-20 | Antimicrobial and antiviral composition having improved sterilizing power |
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| Publication Number | Publication Date |
|---|---|
| CN101347126A true CN101347126A (en) | 2009-01-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CNA2007101816125A Pending CN101347126A (en) | 2007-07-20 | 2007-10-19 | Sterilizing and virus-eradicating composition with improved sterilizing capability |
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| KR (1) | KR20090009442A (en) |
| CN (1) | CN101347126A (en) |
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| KR20090009442A (en) | 2009-01-23 |
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