CN101341929B - Method of preparing feedstuff enzyme agent - Google Patents
Method of preparing feedstuff enzyme agent Download PDFInfo
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- CN101341929B CN101341929B CN2008100716355A CN200810071635A CN101341929B CN 101341929 B CN101341929 B CN 101341929B CN 2008100716355 A CN2008100716355 A CN 2008100716355A CN 200810071635 A CN200810071635 A CN 200810071635A CN 101341929 B CN101341929 B CN 101341929B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a preparation method of feed enzyme agent, relates to biological ferment, and provides a preparation method of feed enzyme agent. Cleaned, sunned and cut fruit and vegetables are refined; water and sugar are added to be stirred; wind is added to be stirred; the product is naturally fermented, filtered and deslagged to obtain stock solution; sirup or sugar and water are added into the stock solution to be evenly stirred and aerated, to obtain primary enlarged culture solution; sirup or sugar and water are added into the primary enlarged culture solution to be evenly stirred and aerated, to obtain secondary enlarged culture solution; sirup or sugar and water are added into the primary enlarged culture solution to be evenly stirred and aerated, and green copperas monohydrate, zinc sulfate monohydrate, manganous sulfate monohydrate and salt are added in to be evenly stirred and aerated, to obtain the feed enzyme agent. The raw materials are natural fruit and vegetables which are easily available; the product is naturally fermented under normal temperature and normal pressure, so the conditions are easily controllable; the process is simple, the equipment cost is low and the cost is low; the product can be applied in livestock-raising, environment protection, water purification, etc. and is easy to be mastered by and acceptable by the peasant household.
Description
Technical field
The present invention relates to a kind of biologic ferment, especially relate to a kind of preparation method of feedstuff enzyme agent.
Background technology
Biologic ferment can be used for the processing of rubbish, as the additive of cleaning product, also can be used for the cultivation of antibody gene enzyme.
Beijing Union Univ. and Cheng Jianfang provide a kind of picture-dismounting liquid made from bioenzyme and take off the method for mounting decontamination in the patent of invention of publication number for CN1113516.This picture-dismounting liquid includes the lysozyme aqueous solution, a-amylase aqueous solution; The compound aqueous solution of the compound aqueous solution of lysozyme, carbohydrase, trypsase, bacillus subtilis neutral proteinase; The compound aqueous solution of lysozyme, carbohydrase, the compound aqueous solution of a-amylase, trypsase, bacillus subtilis neutral proteinase.It is taken off the method for mounting and is divided into dry method and wet method, and dry method is a brushing-constant temperature method, and wet method is an infusion method, can take off " the archives brick " that destroyed by dirts such as microorganism secretion thing, dust, binding agents and mount decontamination, has quick, convenient, safe, economic advantage.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of feedstuff enzyme agent.
The present invention includes following steps:
1) fruits and vegetables is cleaned, airing, chopping;
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the water that accounts for fruits and vegetables gross mass 15%～20%, add the sugar that accounts for fruits and vegetables gross mass 5%～10% again, stir, add the wine that accounts for fruits and vegetables gross mass 0.5%～0.1% again, stir, filter and remove residue after the spontaneous fermentation gets stoste;
3) in step 2) add molasses or sugar in the stoste of gained, and water is mixed thoroughly, aeration, once enlarge nutrient solution;
4) in the once expansion nutrient solution of step 3) gained, add molasses or brown sugar, and water, to mix thoroughly, aeration gets secondary and enlarges nutrient solution;
5) enlarge adding molasses or brown sugar in the nutrient solution at secondary, and water, to mix thoroughly, aeration adds ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and salt again, mixes thoroughly, and aeration gets feedstuff enzyme agent.
In step 1), at least 15 kinds of described fruit, at least 10 kinds of vegetables, vegetables can be adopted vegetables such as leaf vegetables and melon beans; Press mass ratio, fruit: vegetables=1: (0.3～0.6) is preferably fruit: vegetables=1: (0.4～0.5).
In step 2) in, described water is preferably well water, and described sugar is preferably brown sugar, the time of stirring after the sugaring is preferably 3～5 days, and described wine is preferably 38 ° of rice wine, adds the temperature that stirs after drinking and is preferably 28～35 ℃, the time of stirring is preferably 10～15 days, preferably stirs every day 2 times;
In step 3), by mass percentage, the addition of molasses or sugar is for once enlarging 25%～30% of nutrient solution gross mass, the addition of water is for once enlarging 40%～50% of nutrient solution gross mass, the temperature of aeration is preferably 28～35 ℃, the time of aeration is preferably 10～15 days, and preferably 8h/ days, described water was preferably well water.
In step 4), by mass percentage, the addition of molasses or brown sugar is 25%～35% of a secondary expansion nutrient solution gross mass, the addition of water is 35%～45% of a secondary expansion nutrient solution gross mass, best 10～12 days of the time of aeration, preferably 8h/ days, best 28～35 ℃ of the temperature of aeration, preferably be placed on shading, shady and cool place, described water is preferably well water.
In step 5), by mass percentage, the addition of molasses or brown sugar is 25%～30% of a feedstuff enzyme agent gross mass, the addition of water is 50%～65% of a feedstuff enzyme agent gross mass, the addition of ferrous sulfate monohydrate is 0.2%～0.4% of a feedstuff enzyme agent gross mass, the addition of monohydrate zinc sulphate is 0.2%～0.4% of a feedstuff enzyme agent gross mass, the addition of manganese sulfate monohydrate is 0.09%～0.1% of a feedstuff enzyme agent gross mass, the addition of salt is 0.3%～0.4% of a feedstuff enzyme agent gross mass, and described salt is preferably salt compounded of iodine (commercially available); Enlarge at secondary and to add molasses or brown sugar in the nutrient solution, and the temperature of the aeration behind the water is preferably 28～35 ℃, the time of aeration is preferably 10 days, and preferably 8h/ days, described water was preferably well water; The temperature of adding the aeration after ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and the salt again is preferably 28～35 ℃, and the time of aeration is preferably 10 days, preferably 8h/ days.
Leftover bits and pieces when molasses are sugar refinery production sucrose.
Compare with existing feed addictive, outstanding advantage of the present invention and outstanding effect are as follows:
1. raw material sources are easy to obtain in the fruits and vegetables of nature;
2. spontaneous fermentation under normal temperature, normal pressure, condition are easy to control;
3. production technology is simple, and the production equipment cost is low, and production cost is low;
4. product can be applicable to livestock-raising, environmental protection, purifies water etc., and peasant household is easy to grasp and accept, aspect livestock-raising: can improve immunity, reduce disease, strengthen premunition, improve survival rate, promote growth, reduce medication, improve quality, improve physical constitution; Aspect environmental protection: the absorbing environmental toxic gas, reduce stink, purify water, improve environment, improve environmental quality.
The specific embodiment
Following examples will the present invention is further illustrated.
Embodiment 1
1) fruits and vegetables is cleaned, airing, chopping, fruit is selected apple, plum, pears, dragon fruit, durian, wax-apple, peach, banana, lichee, longan, watermelon, "Hami" melon, pineapple, Chinese date, grape, sugarcane, pawpaw, Kiwi berry, water chestnut, strawberry and red bayberry for use, vegetables are selected Chinese cabbage, cucumber, wild cabbage, cauliflower, spinach, Kidney bean, sponge gourd, cucurbit, leaf mustard, rape, wax gourd, pumpkin, celery, three-coloured amaranth and leek for use, press mass ratio, fruit: vegetables=1: 0.3.
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the well water that accounts for fruits and vegetables gross mass 18%, add the brown sugar that accounts for fruits and vegetables gross mass 8% again, stirred 3 days, add 38 ° of rice wine that account for fruits and vegetables gross mass 0.3% again, stir, the temperature of stirring is 30 ℃, the time of stirring is 12 days, stir every day 2 times, filter and remove residue after the spontaneous fermentation gets stoste.
3) in step 2) add molasses and well water in the stoste of gained, mix thoroughly, aeration, must once enlarge nutrient solution, by mass percentage, the addition of molasses is for once enlarging 25% of nutrient solution gross mass, the addition of well water is for once enlarging 45% of nutrient solution gross mass, the temperature of aeration is 30 ℃, and the time of aeration is 13 days, preferably 8h/ days.
4) in the once expansion nutrient solution of step 3) gained, add molasses and well water, mix thoroughly, aeration gets secondary and enlarges nutrient solution, by mass percentage, the addition of molasses is 30% of a secondary expansion nutrient solution gross mass, the addition of well water is 35% of a secondary expansion nutrient solution gross mass, and the time of aeration is 11 days, preferably 8h/ days, the temperature of aeration is 32 ℃, is placed on shading, shady and cool place.
5) enlarge adding molasses and well water in the nutrient solution at secondary, mix thoroughly, aeration, add ferrous sulfate monohydrate again, monohydrate zinc sulphate, manganese sulfate monohydrate and salt, mix thoroughly, aeration, get feedstuff enzyme agent, by mass percentage, the addition of molasses is 30% of a feedstuff enzyme agent gross mass, the addition of well water is 50% of a feedstuff enzyme agent gross mass, the addition of ferrous sulfate monohydrate is 0.3% of a feedstuff enzyme agent gross mass, and the addition of monohydrate zinc sulphate is 0.4% of a feedstuff enzyme agent gross mass, and the addition of manganese sulfate monohydrate is 0.095% of a feedstuff enzyme agent gross mass, the addition of salt is 0.35% of a feedstuff enzyme agent gross mass, and salt is selected commercially available salt compounded of iodine for use; The temperature that enlarges the aeration after adding molasses and well water in the nutrient solution at secondary is 30 ℃, the time of aeration is 10 days, and 8h/ days, the temperature of adding the aeration after ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and the salt again was 30 ℃, the time of aeration is 10 days, 8h/ days.
Leftover bits and pieces when molasses are sugar refinery production sucrose.
Embodiment 2
1) fruits and vegetables is cleaned, airing, chopping, fruit is selected tangerine, wax-apple, peach, banana, lichee, longan, watermelon, "Hami" melon, pineapple, Chinese date, grape, sugarcane, pawpaw, Kiwi berry, water chestnut, strawberry, red bayberry, apricot, shaddock, pears, loquat and bajiao banana for use, vegetables are selected Chinese cabbage, cucumber, wild cabbage, cauliflower, spinach, Kidney bean, sponge gourd, cucurbit, carrot, leaf mustard, rape, wax gourd, pumpkin, cucumber, celery, three-coloured amaranth and leek for use, press mass ratio, fruit: vegetables=1: 0.4.
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the well water that accounts for fruits and vegetables gross mass 17%, add the white sugar that accounts for fruits and vegetables gross mass 6% again, stirred 4 days, add the kaoliang spirit that accounts for fruits and vegetables gross mass 0.5% again, adding the temperature that stirs after drinking is 28 ℃, stirs 10 days, stirs every day 2 times, filter and remove residue after the spontaneous fermentation gets stoste.
3) in step 2) add white sugar and well water in the stoste of gained, mix thoroughly, aeration, must once enlarge nutrient solution, by mass percentage, the addition of white sugar is for once enlarging 28% of nutrient solution gross mass, the addition of well water is for once enlarging 40% of nutrient solution gross mass, the temperature of aeration is 32 ℃, and the time of aeration is 12 days, 8h/ days.
4) in the once expansion nutrient solution of step 3) gained, add brown sugar and well water, mix thoroughly, aeration gets secondary and enlarges nutrient solution, by mass percentage, the addition of brown sugar is 35% of a secondary expansion nutrient solution gross mass, the addition of well water is 38% of a secondary expansion nutrient solution gross mass, and the time of aeration is 10 days, 8h/ days, the temperature of aeration is 28 ℃, is placed on shading, shady and cool place.
5) enlarge adding brown sugar and well water in the nutrient solution at secondary, mix thoroughly, aeration, add ferrous sulfate monohydrate again, monohydrate zinc sulphate, manganese sulfate monohydrate and salt, mix thoroughly, aeration, get feedstuff enzyme agent, by mass percentage, the addition of brown sugar is 25% of a feedstuff enzyme agent gross mass, and the addition of well water is 55% of a feedstuff enzyme agent gross mass, and the addition of ferrous sulfate monohydrate is 0.2% of a feedstuff enzyme agent gross mass, the addition of monohydrate zinc sulphate is 0.35% of a feedstuff enzyme agent gross mass, the addition of manganese sulfate monohydrate is 0.097% of a feedstuff enzyme agent gross mass, and the addition of salt is 0.36% of a feedstuff enzyme agent gross mass, and salt is selected commercially available salt compounded of iodine for use, the temperature that enlarges the aeration after adding brown sugar and well water in the nutrient solution at secondary is 28 ℃, the time of aeration is 10 days, 8h/ days, adds ferrous sulfate monohydrate again, monohydrate zinc sulphate, the temperature of the aeration after manganese sulfate monohydrate and the salt is 28 ℃, the time of aeration is 10 days, 8h/ days.
Embodiment 3
1) fruits and vegetables is cleaned, airing, chopping, fruit is selected plum, pears, dragon fruit, orange, wax-apple, peach, banana, lichee, longan, watermelon, "Hami" melon, pineapple, Chinese date, grape, sugarcane, pawpaw, Kiwi berry, water chestnut, strawberry, red bayberry and apricot for use, vegetables are selected Chinese cabbage, cucumber, wild cabbage, cauliflower, spinach, Kidney bean, sponge gourd, cucurbit, leaf mustard, rape, wax gourd, pumpkin, cucumber, celery, three-coloured amaranth and leek for use, press mass ratio, fruit: vegetables=1:0.5.
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the water that accounts for fruits and vegetables gross mass 15%, add the brown sugar that accounts for fruits and vegetables gross mass 10% again, stirred 5 days, add 38 ° of rice wine that account for fruits and vegetables gross mass 0.4% again, adding the temperature that stirs after drinking is 32 ℃, stirs 13 days, stirs every day 2 times, filter and remove residue after the spontaneous fermentation gets stoste.
3) in step 2) add brown sugar and well water in the stoste of gained, mix thoroughly, aeration, must once enlarge nutrient solution, by mass percentage, the addition of brown sugar is for once enlarging 27% of nutrient solution gross mass, the addition of well water is for once enlarging 46% of nutrient solution gross mass, the temperature of aeration is 28 ℃, and the time of aeration is 10 days, 8h/ days.
4) in the once expansion nutrient solution of step 3) gained, add brown sugar and well water, mix thoroughly, aeration gets secondary and enlarges nutrient solution, by mass percentage, the addition of brown sugar is 25% of a secondary expansion nutrient solution gross mass, the addition of well water is 42% of a secondary expansion nutrient solution gross mass, and the time of aeration is 11 days, 8h/ days, the temperature of aeration is 30 ℃, is placed on shading, shady and cool place.
5) enlarge adding molasses and well water in the nutrient solution at secondary, mix thoroughly, aeration, add ferrous sulfate monohydrate again, monohydrate zinc sulphate, manganese sulfate monohydrate and salt, mix thoroughly, aeration, get feedstuff enzyme agent, by mass percentage, the addition of molasses is 27% of a feedstuff enzyme agent gross mass, the addition of water is 60% of a feedstuff enzyme agent gross mass, the addition of ferrous sulfate monohydrate is 0.25% of a feedstuff enzyme agent gross mass, and the addition of monohydrate zinc sulphate is 0.2% of a feedstuff enzyme agent gross mass, and the addition of manganese sulfate monohydrate is 0.09% of a feedstuff enzyme agent gross mass, the addition of salt is 0.3% of a feedstuff enzyme agent gross mass, and salt is selected commercially available salt compounded of iodine for use; The temperature that enlarges the aeration after adding molasses and well water in the nutrient solution at secondary is 32 ℃, the time of aeration is 10 days, and 8h/ days, the temperature of adding the aeration after ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and the salt again was 32 ℃, the time of aeration is 10 days, 8h/ days.
Embodiment 4
1) fruits and vegetables is cleaned, airing, chopping, fruit is selected apple, plum, pears, wax-apple, peach, banana, lichee, mandarin orange, watermelon, "Hami" melon, pineapple, Chinese date, grape, sugarcane, pawpaw, Kiwi berry, water chestnut, strawberry, red bayberry, apricot, loquat and bajiao banana for use, vegetables are selected Chinese cabbage, cucumber, wild cabbage, cauliflower, spinach, Kidney bean, sponge gourd, cucurbit, leaf mustard, rape, wax gourd, pumpkin, celery, three-coloured amaranth and leek for use, press mass ratio, fruit: vegetables=1: 0.6.
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the water that accounts for fruits and vegetables gross mass 20%, add the molasses that account for fruits and vegetables gross mass 5% again, stirred 4 days, add 35 ° of kaoliang spirits that account for fruits and vegetables gross mass 0.1% again, stirred 15 days, and stirred every day 2 times, adding the temperature that stirs after drinking is 35 ℃, filter and remove residue after the spontaneous fermentation gets stoste.
3) in step 2) add molasses and water in the stoste of gained, mix thoroughly, aeration, must once enlarge nutrient solution, by mass percentage, the addition of molasses is for once enlarging 30% of nutrient solution gross mass, the addition of water is for once enlarging 50% of nutrient solution gross mass, the temperature of aeration is 35 ℃, and the time of aeration is 15 days, 8h/ days.
4) in the once expansion nutrient solution of step 3) gained, add brown sugar and water, mix thoroughly, aeration gets secondary and enlarges nutrient solution, by mass percentage, the addition of brown sugar is 28% of a secondary expansion nutrient solution gross mass, the addition of water is 45% of a secondary expansion nutrient solution gross mass, the 12 days time of aeration, 8h/ days, the temperature of aeration is 35 ℃, is placed on shading, shady and cool place.
5) enlarge adding brown sugar and water in the nutrient solution at secondary, mix thoroughly, aeration, add ferrous sulfate monohydrate again, monohydrate zinc sulphate, manganese sulfate monohydrate and salt, mix thoroughly, aeration, get feedstuff enzyme agent, by mass percentage, the addition of brown sugar is 28% of a feedstuff enzyme agent gross mass, the addition of water is 65% of a feedstuff enzyme agent gross mass, and the addition of ferrous sulfate monohydrate is 0.4% of a feedstuff enzyme agent gross mass, and the addition of monohydrate zinc sulphate is 0.3% of a feedstuff enzyme agent gross mass, the addition of manganese sulfate monohydrate is 0.1% of a feedstuff enzyme agent gross mass, the addition of salt is 0.4% of a feedstuff enzyme agent gross mass, and the temperature that enlarges the aeration after adding brown sugar and water in the nutrient solution at secondary is 35 ℃, and the time of aeration is 10 days, 8h/ days, add ferrous sulfate monohydrate again, monohydrate zinc sulphate, the temperature of the aeration after manganese sulfate monohydrate and the salt is 35 ℃, and the time of aeration is 10 days, 8h/ days.
Claims (6)
1. the preparation method of a feedstuff enzyme agent is characterized in that may further comprise the steps:
1) fruits and vegetables is cleaned, airing, chopping;
2) will clean, the fruits and vegetables defibrination behind the airing, chopping, by mass percentage, add the water that accounts for fruits and vegetables gross mass 15%～20%, add the sugar that accounts for fruits and vegetables gross mass 5%～10% again, stir, add the wine that accounts for fruits and vegetables gross mass 0.1%～0.5% again, stir, filter and remove residue after the spontaneous fermentation, get stoste, the time of stirring after the sugaring is 3～5 days, and adding the temperature that stirs after drinking is 28～35 ℃, the time of stirring is 10～15 days, stirs every day 2 times;
3) in step 2) add molasses or sugar in the stoste of gained, and water is mixed thoroughly, aeration, once enlarge nutrient solution, the temperature of aeration is 28～35 ℃, the time of aeration is 10～15 days;
4) in the once expansion nutrient solution of step 3) gained, add molasses or brown sugar, and water, to mix thoroughly, aeration gets secondary and enlarges nutrient solution, and the time of aeration is 10～12 days, and the temperature of aeration is 28～35 ℃;
5) enlarge adding molasses or brown sugar in the nutrient solution at secondary, and water, mix thoroughly, aeration, add ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and salt again, mix thoroughly, aeration, get feedstuff enzyme agent, enlarges at secondary and to add molasses or brown sugar in the nutrient solution, and the temperature of the aeration behind the water is 28～35 ℃ that the time of aeration is 10 days, the temperature of adding the aeration after ferrous sulfate monohydrate, monohydrate zinc sulphate, manganese sulfate monohydrate and the salt again is 28～35 ℃, and the time of aeration is 10 days.
2. the preparation method of a kind of feedstuff enzyme agent as claimed in claim 1 is characterized in that in step 1), at least 15 kinds of described fruit, at least 10 kinds of vegetables.
3. the preparation method of a kind of feedstuff enzyme agent as claimed in claim 1 is characterized in that pressing mass ratio in step 1), fruit: vegetables=1: 0.3～0.6.
4. the preparation method of a kind of feedstuff enzyme agent as claimed in claim 1, it is characterized in that in step 3), by mass percentage, the addition of molasses or sugar is for once enlarging 25%～30% of nutrient solution gross mass, and the addition of water is for once enlarging 40%～50% of nutrient solution gross mass.
5. the preparation method of a kind of feedstuff enzyme agent as claimed in claim 1, it is characterized in that in step 4), by mass percentage, the addition of molasses or brown sugar is 25%～35% of a secondary expansion nutrient solution gross mass, and the addition of water is 35%～45% of a secondary expansion nutrient solution gross mass.
6. the preparation method of a kind of feedstuff enzyme agent as claimed in claim 1, it is characterized in that in step 5), by mass percentage, the addition of molasses or brown sugar is 25%～30% of a feedstuff enzyme agent gross mass, the addition of water is 50%～65% of a feedstuff enzyme agent gross mass, the addition of ferrous sulfate monohydrate is 0.2%～0.4% of a feedstuff enzyme agent gross mass, the addition of monohydrate zinc sulphate is 0.2%～0.4% of a feedstuff enzyme agent gross mass, the addition of manganese sulfate monohydrate is 0.09%～0.1% of a feedstuff enzyme agent gross mass, and the addition of salt is 0.3%～0.4% of a feedstuff enzyme agent gross mass.
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