CN101339153B - Labelling foodstuff pathogenic bacteria growth checking method - Google Patents
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Abstract
The invention discloses a rapid detection method which has the characteristics of low selectivity, no specificity and interactive sensitivity. The method can be used for accurately, sensitively, simply, economically and rapidly tracing the growth of pathogenic bacteria of food and the integrated information of metabolism thereof. In the method, bacteria are inoculated; the bacteria can be used for inactivating culture solution after being cultured for different periods of time; an intelligent chemical analysis system of multi-frequency wide-breadth pulse is used for detecting the inactivated culture; response signals are collected and analyzed; other steps also comprised in the method. The method uses an intelligent multi-frequency pulse scanner based on multi-frequency wide-breadth pulse voltammetry, and combines a three-electrode system which comprises a working electrode array, so as to collect the integrated information of the growth of pathogenic bacteria of food in the way which has the characteristics of low selectivity, no specificity and interactive sensitivity; then the method of principal component analysis is used for processing the collected information so as to trace the growth and metabolism of the pathogenic bacteria of food in the simple, accurate and sensitive way.
Description
Technical field
The present invention relates to the significantly method of pulse intelligent chemical analysis system food-borne pathogens growing state in the monitoring liquid nutrient culture media of the rapid detection technical field of spike bacterial growth situation, particularly multifrequency.
Background technology
In recent years, the diseases such as food poisoning that cause of foodstuff pathogenic bacteria are subject to society and show great attention to.Escherichia coli O 157: H7 (E.coli O157:H7), staphylococcus aureus (Staphylococcus Aureus), salmonella typhimurium (Salmonella typhimurium) etc. are considered to the maximum food-borne pathogens of several harm, they have not only caused huge economic loss, and also serious threat is to human health and safety.Therefore, in the processes such as food production, processing, storage and sale, labelling foodstuff pathogenic bacteria growth situation and rule thereof and toxic metabolite product produce the integrated information of rule, detect significant to safety in production and the health that instructs food.
At present, the detection method of all labelling foodstuff pathogenic bacteria growth situations and trend thereof, comprise traditional National Standard Method and other method for quick and new technology, all can only measure to thalline quantity or to the Changing Pattern of a certain composition such as ATP equal size, can not react in the thalli growth process the consumption situation of nutrient matrix, the integrated information of metabolic alterations rule.Therefore, it is particularly important to set up a kind of method for quick of accurate, sensitive, easy and economic labelling foodstuff pathogenic bacteria growth metabolism status integrated information.
Bacterial classification is different, and self contained enzyme system is different, and metabolic way is just not identical with metabolic pathway, and is also not identical to the product of the decomposition utilization of nutriment and generation.Even same thalline, and even the different growing stage of homophyletic even clone's thalline, in the environment of same restriction, utilize degree and metabolic product to matrix also are not quite similar, utilize multifrequency significantly the pulse sensor-based system can analyze integrated information with these variations of spike, and have the impact of the color sample of not being subjected to, turbidity, sample can be not treated, the advantages such as it is lossless to detect front and back, and instrument equipment is relatively simple.
At present, the research of useful immunosensor, quartz crystal piezoelectric sensor other biological Sensor monitoring food microorganisms report, these methods all exist key technology problems not to be resolved, thereby have limited its development and application.Be to be the domestic and international forward position of research with highly sensitive electronic tongues Fast Evaluation quality and safety of food, only be applied at present detect the fields such as heavy metal pollution in food and the agricultural product, residues of pesticides and authenticity of products identification.
Intelligent chemical analysis system (so-called electronic tongues) by the different chemical substance of sensor array (being equivalent to the tongue in the biosystem) impression, gathers various signal message input computer.Computer has replaced the cerebral function in the biosystem, carries out analyzing and processing by software, distinguishes the global feature of identification heterogeneity material.
Number of patent application be 200710068869.X Patent Application Publication a kind of intelligent chemical analysis system for fluid sample, comprise sensor array, signal excitation harvester and intellectual analysis unit, signal excitation harvester is added to auxiliary electrode with predetermined excitation pulse sequence, the response signal of simultaneously collecting work electrode generation; The intellectual analysis unit comprises signal excitation harvester host computer and intelligent data analysis software, is used for response signal is processed and analyzed; The signal excitation harvester of this intelligent chemical analysis system is multifrequency pulse excitation harvester, can produce the significantly pulse electromotive force of multi-frequency section; Its action step is as follows: by Single-chip Controlling DAC D/A switch module, add to auxiliary electrode behind the pumping signal input potentiostat 1.; 2. the electric current of working electrode generation is converted to voltage signal by the programmable current follower, enters inverting amplifier; 3. by the noise in the low-pass filtering removal signal, input ADC mould/number conversion module converts becomes digital quantity to feed back to single-chip microcomputer.
Because it is the signal excitation drainage pattern that this intelligent chemical analysis system adopts the multifrequency pulse voltammetry, response signal is strong compared to existing technology, and the noise signal to noise ratio is high, and quantity of information is abundanter; Adopted simultaneously to be fit to that himself data structure characteristic uses, convenient, intelligentized intellectual analysis software have that response signal is strong, signal stabilization, and the noise signal to noise ratio is high, the characteristics such as quantity of information is abundant, resolution is high, easy operating.
Utilize this intelligent chemical analysis system to have non-specific, weak selectivity, (organic and inorganic to different component in the solution, ion and nonionic) have the height cross sensitivity characteristic, bacteria group culture liquid to different growing stage detects, process in conjunction with suitable algorithm for pattern recognition and multivariable technique pair array data, obtain nutrient solution sample qualitative, quantitative information, thereby can monitor the growing state of flora.
Summary of the invention
The object of the present invention is to provide a kind of accurate, sensitive, easy, efficient, economic labelling foodstuff pathogenic bacteria growth and the detection method of metabolism integrated information, namely a kind of with the multifrequency method of pulse intelligent chemical analysis system monitoring flora growth significantly.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of detection method of labelling foodstuff pathogenic bacteria growth is characterized in that may further comprise the steps:
A. with bacterial classification inoculation to be measured in 1% glucose broth, static cultivation 18 hours, the preparation seed liquor;
B. with seed liquor respectively equivalent transfer in the triangular flask that the 50ml1% glucose broth is housed, cultivate respectively 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 24h, 30h after, nutrient solution is carried out deactivation;
C. multifrequency significantly the signal excitation harvester host computer of pulse intelligent chemical analysis system select signal excitation harvester required voltage, pulsed frequency, interpulse period;
D. multifrequency significantly the sensor array of pulse intelligent chemical analysis system immerse in the nutrient solution, described sensor array with non-modified metal electrodes such as platinum electrode, gold electrode, palladium electrode, nickel electrode, tungsten electrode, titanium electrodes as working electrode, platinum electrode is as auxiliary electrode, and Ag/AgCl is as contrast electrode;
E. apply voltage at working electrode, each working electrode in the sensor array is carried out the pulsatile once sequence;
F. gather current responsing signal, extract summit and flex point value as the variable of test sample, with multifrequency significantly the intelligent data analysis software of pulse intelligent chemical analysis system carry out analyzing and processing.
Further, food pathogenic of the present invention can be staphylococcus aureus, and described multifrequency significantly sensor array and the pulse excitation frequency band of pulse intelligent chemical analysis system is: the 10Hz frequency band of the 100Hz frequency band of Wu electrode, the 1Hz frequency band of Pt electrode, Ag electrode, the 10Hz frequency band of Ti electrode.
Food pathogenic of the present invention can also be Escherichia coli O 157: H7, and described multifrequency significantly sensor array and the pulse excitation frequency band of pulse intelligent chemical analysis system is: the 100Hz frequency band of Au electrode, the 1Hz frequency band of Pt electrode.
Food pathogenic of the present invention can also be bacillus subtilis, and described multifrequency significantly sensor array and the pulse excitation frequency band of pulse intelligent chemical analysis system is: the 1Hz of Ba electrode, 10Hz, three frequency bands of 100Hz.
Further, the pulse height of each pulsed frequency section all from 1.0V, then change 0.2V at every turn until-1.0V, between two different frequency bands, insert the 5s time interval.
The present invention adopt multifrequency significantly pulse as exciting sweep signal, detect the Whole Response signal of foodstuff pathogenic bacteria growth process, the multifrequency that forms three electrod-array systems with multiple different non-modified metal electrode is the pulse sensor-based system significantly, the Growth and metabolism rule of the various bacterium of spike.
The multifrequency that the present invention adopts is the pulse intelligent chemical analysis system significantly, the sensor electrode array can comprise that platinum electrode, gold electrode, palladium electrode, nickel electrode, tungsten electrode, titanium electrode are as working electrode, platinum electrode is as auxiliary electrode, Ag/AgCl is as contrast electrode, a plurality of standard three-electrode systems of composition.
Signal excitation harvester adopts significantly pulse voltammetry of multifrequency.Multifrequency significantly pulse excitation current potential is comprised of 1Hz, 10Hz and three pulsed frequency sections of 100Hz, and being equivalent to respectively pulse interval is 1s, 0.1s, the significantly pulse voltammetry of 0.01s.The pulse height of the significantly pulse of each pulsed frequency section all adopts the identical pulse changes in amplitude, from 1.0V, then change at every turn 0.2V until-1.0V.Between two different frequency bands, insert the 5s time interval, can eliminate the sluggish interference of response between each frequency.
The intellectual analysis unit adopts statistical method relatively more commonly used in the multivariate statistical analysis, be principal component analytical method (principal component analysis, PCA) to multifrequency significantly the data of pulse sensor-based system collection process, the summit of extraction current acquisition signal and flex point value are as the variable of test sample, by the shot chart that principal component analytical method obtains, different test sample is distinguished, identified.At first, with the vectorial representative sample of row, vertically amount represents variable the data of Different electrodes different frequency section is preserved into respectively data form, carries out principal component analysis (PCA).Then, the principal component scores figure that compares Different electrodes, compare each electrode differentiation effect to sample under different frequency on the two and three dimensions plane, the electrode data lateral stacking that principal component scores figure is had complementation, reformulation is with the vectorial representative sample of row, vertically amount represents the data array of variable, the final searching has principal component scores figure and the electrode data stacked system of preferably distinguishing effect, seek to distinguish the optimal electrod-array array mode of this class sample and the optimal multifrequency pulse frequency band of electrode, finally determine that suitable 2-3 electrode and pulsed frequency section thereof are as the monitoring system of monitoring purpose bacteria growing situation.
The present invention adopt multifrequency significantly the pulse intelligent chemical analysis system bacteria group culture liquid of different growing stage is detected in real time, the response message of the multinomial overall target of complicated ingredient that constantly changes in the collection liquid nutrient culture media, carry out the relevant information analysis in conjunction with the principal component analytical method in the multivariate statistical analysis, select the sensor array and the frequency band that are suitable for monitoring bacterium, particularly staphylococcus aureus, Escherichia coli O 157: H7 and bacillus subtilis growing state, greatly strengthened precision of analysis.The sensor array and the frequency band that are suitable for monitoring the staphylococcus aureus growth situation are: the 10Hz frequency band of the 100Hz frequency band of Wu electrode, the 1Hz frequency band of Pt electrode, Ag electrode, the 10Hz frequency band of Ti electrode etc.Suitable sensor array and the frequency band of Escherichia coli O 157: H7 are: the 100Hz frequency band of Au electrode, the 1Hz frequency band of Pt electrode.The sensor array and the frequency band that are suitable for monitoring bacillus subtilis are: the 1Hz of Ba electrode, 10Hz, three frequency bands of 100Hz.
The present invention is to be the analysis of modernization detecting instrument of characteristics by a kind of solution overall quality characteristic synthetic information that can detect---based on the multifrequency of electrod-array pulse sensor-based system significantly, in conjunction with the principal component analytical method in the Multielement statistical analysis method, foodstuff pathogenic bacteria growth metabolism integrated information and Changing Pattern thereof are carried out spike detect and consist of.Have low selectivity, non-specific and mutual susceptibility, accurately, characteristics and the advantage such as sensitive, economical and quick.
Description of drawings
Fig. 1 is the significantly principal component scores figure of pulse intelligent chemical analysis system detection staphylococcus aureus different times nutrient solution of multifrequency; Wherein, (a) the Wu electrode at the principal component scores figure of 100Hz frequency band; (b) the Pt electrode is at the principal component scores figure of 1Hz frequency band; (c) the Ag electrode must be schemed in the major component of 10Hz frequency band; (d) the Ti electrode is at the principal component scores figure of 10Hz frequency band.
Fig. 2 is significantly pulse intelligent chemical analysis system detection Escherichia coli O 157 of multifrequency: the principal component scores figure of H7 different times nutrient solution; Wherein, (a) the Pt electrode at the principal component scores figure of 1Hz frequency band; (b) the Au electrode is at the principal component scores figure of 100Hz frequency band;
Fig. 3 is the significantly principal component scores figure of pulse intelligent chemical analysis system detection bacillus subtilis different times nutrient solution of multifrequency; Wherein, (a) the Ba electrode at the principal component scores figure of 1Hz frequency band; (b) the Ba electrode is at the principal component scores figure of 10Hz frequency band; (c) the Ba electrode is at the principal component scores figure of 100Hz frequency band.
Embodiment
Embodiment 1: the staphylococcus aureus growth situation analysis
In 1% glucose broth, static cultivation 18～24h prepares seed liquor with the staphylococcus aureus bacterial classification inoculation.
With seed liquor respectively equivalent transfer in the triangular flask that the 50ml1% glucose broth is housed, after cultivating respectively 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 24h, 30h under 37 ℃, to 100 ℃ of nutrient solutions, 40min water-bath deactivation.
With multifrequency significantly the nutrient solution of pulse intelligent chemical analysis system after to deactivation detect.It is working electrode that sensor array is selected Wu electrode, Pt electrode, Ag electrode, Ti electrode, and platinum electrode is as auxiliary electrode, and Ag/AgCl is as contrast electrode.
Multifrequency significantly pulse excitation current potential is comprised of 1Hz, 10Hz and three pulsed frequency sections of 100Hz, and being equivalent to respectively pulse interval is 1s, 0.1s, the significantly pulse voltammetry of 0.01s.The pulse height of the significantly pulse of each pulsed frequency section all adopts the identical pulse changes in amplitude, from 1.0V, then change at every turn 0.2V until-1.0V.Between two different frequency bands, insert the 5s time interval, can eliminate the sluggish interference of response between each frequency.
The sensor array of intelligent chemical analysis system put into cultivate behind 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 24h, the 30h and the nutrient solution of deactivation, apply voltage at working electrode, each working electrode in the sensor array is carried out the pulsatile once sequence.Follow-on test three times, data are averaged, behind the complete fluid to be measured of every detection, to electrod-array polishing, distilled water flushing; Contrast electrode cleans with distilled water, then blots with filter paper.
With principal component analytical method (principal component analysis, PCA) to multifrequency significantly the data of pulse sensor-based system collection process, the summit of extraction current acquisition signal and flex point value are as the variable of test sample, by the shot chart that principal component analytical method obtains, different test sample is distinguished, identified.At first, with the vectorial representative sample of row, vertically amount represents variable the data of Different electrodes different frequency section is preserved into respectively data form, carries out principal component analysis (PCA).Then, the principal component scores figure that compares Different electrodes, compare each electrode differentiation effect to sample under different frequency on the two and three dimensions plane, the electrode data lateral stacking that principal component scores figure is had complementation, reformulation is with the vectorial representative sample of row, vertically amount represents the data array of variable, the final searching has principal component scores figure and the electrode data stacked system of preferably distinguishing effect, seek and distinguish the optimal electrod-array array mode of this class sample and the optimal multifrequency pulse frequency band of electrode, finally determine Wu electrode 100Hz frequency band, Pt electrode 1Hz frequency band, Ag electrode 10Hz frequency band, Ti electrode 10Hz frequency band is as the monitoring system of monitoring staphylococcus aureus growth situation.
As shown in Figure 1, (a) the Wu electrode at the principal component scores figure of 100Hz frequency band; (b) the Pt electrode is at the principal component scores figure of 1Hz frequency band; (c) the Ag electrode must be schemed in the major component of 10Hz frequency band; (d) the Ti electrode is at the principal component scores figure of 10Hz frequency band.
From Fig. 1 (a), can find out that the Wu electrode mainly is a growth tendency that embodies at composition two at the 100Hz frequency band.(d) the 10Hz frequency band that shows the Ti electrode in has obvious growth tendency at composition two; (b) and (c), show that the 1Hz frequency band of Pt electrode and the 10Hz frequency band of Ag electrode have significant growth tendency at composition one.Sample during 0h rigidly connects kind not to be cultivated with regard to deactivation, and the sample of it and other growth period has larger dispersion, and this growth metabolism that has shown staphylococcus aureus has produced very large impact to nutrient culture media; About from 1h to 8h, the dispersion of sample is also very large, and this mainly is because staphylococcus aureus has progressed into logarithmic phase after cultivating 2h, and growth metabolism is vigorous especially to be caused; Staphylococcus aureus growth has entered stationary phase behind the 9h, and the Growth and death kept in balance is so the dispersion of the sample when 30h is arrived in the back is less.In these principal component scores figure, be easy to see the growth of staphylococcus aureus.Record the result with traditional nephelometry and compare, fit like a glove, be both at 8h-9h and progress into stationary phase, and growth and metabolism before can showing are very vigorous.Therefore multifrequency significantly the staphylococcus aureus growth situation of pulse sensor-based system in the monitoring liquid nutrient culture media be feasible.
Embodiment 2: colon bacillus 0157: the H7 growing state is analyzed
With Escherichia coli O 157: the H7 bacterial classification inoculation in 1% glucose broth, static cultivation 18～24h, preparation seed liquor.
With seed liquor respectively equivalent transfer in the triangular flask that the 50ml1% glucose broth is housed, cultivate respectively 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 24h, 30h after, nutrient solution is carried out deactivation.
With multifrequency significantly the nutrient solution of pulse intelligent chemical analysis system after to deactivation detect.It is working electrode that sensor array is selected Pt electrode, Au electrode, and platinum electrode is as auxiliary electrode, and Ag/AgCl is as contrast electrode.
Pulse voltage, pulsed frequency, interpulse period, detection method and signal analysis method are identical with specific embodiment 1.
Final definite Pt electrode 1Hz frequency band, Au electrode 100Hz frequency band are as monitoring Escherichia coli O 157: the monitoring system of H7 upgrowth situation.
As shown in Figure 2, multifrequency significantly the pulse intelligent chemical analysis system detect Escherichia coli O 157: the principal component scores figure of H7 different times nutrient solution; Wherein, (a) the Pt electrode at the principal component scores figure of 1Hz frequency band; (b) the Au electrode is at the principal component scores figure of 100Hz frequency band.
The principal component scores figure of the 100Hz frequency band of the 1Hz frequency band of Pt electrode, Au electrode is more similar in Fig. 2.All be that the dispersion on principal component scores figure is larger in front 6h, and at 6h and 7h a flex point arranged, and their dispersion is less behind the 7h.This illustrated Escherichia coli O 157: H7 to be grown in the first five hour large especially on the impact of cultivating, that is to say Escherichia coli O 157: H7 to be grown in this stage active especially; And its growth progresses into stationary phase behind the 7h.The growth that can recognize Escherichia coli O 157: H7 from shot chart has generally reached the peak about cultivation 6h-7h, the thalline breeding in the nutrient culture media is the fastest, and metabolism is the most vigorous.
Embodiment 3: the bacillus subtilis growing state is analyzed
Bacillus subtilis strain is inoculated in 1% glucose broth static cultivation 18～24h, preparation seed liquor.
With seed liquor respectively equivalent transfer in the triangular flask that 50ml 1% glucose broth is housed, cultivate respectively 0h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 13h, 24h, 30h after, nutrient solution is carried out deactivation.
With multifrequency significantly the nutrient solution of pulse intelligent chemical analysis system after to deactivation detect.It is working electrode that sensor array is selected the Ba electrode, and platinum electrode is as auxiliary electrode, and Ag/AgCl is as contrast electrode.
Pulse voltage, pulsed frequency, interpulse period, detection method and signal analysis method are identical with specific embodiment 1.
Final definite Ba electrode 1Hz frequency band, 10Hz frequency band, 100Hz frequency band are as the monitoring system of monitoring the bacillus subtilis upgrowth situation.
As shown in Figure 3, multifrequency significantly the pulse intelligent chemical analysis system detect the principal component scores figure of the different cultivation period nutrient solutions of bacillus subtilis; Wherein, (a), (b), (c) are respectively the principal component scores figure of Ba electrode on 1Hz frequency band, 10Hz frequency band, 100Hz frequency band.
As can be seen from Figure 3, because the sample during 0h is without cultivation, so the dispersion of it and other samples is relatively large.The 10Hz frequency band of Ba electrode and the principal component scores figure of 100Hz show that bacillus subtilis is cultivated about 4h and enter the growth logarithmic phase in 1% glucose broth.In three principal component scores figure, we can find out that the sample variation degree before the 11h is all larger, and namely the growth of bacillus subtilis is active on the impact of nutrient culture media in this time period.And the dispersion less of the sample of the time period of back illustrates that bacillus subtilis has progressed into stationary phase.
The multifrequency that the present invention adopts significantly pulse sensing intelligent chemical analysis system with routine significantly pulse be extended to three kinds of frequencies, and take principal component analytical method as data processing means, the result and the standard method (nephelometry) that obtain in the growth tendency of monitoring food-borne pathogens are basically identical.And and other method relatively, and it has, and simple in structure, stable performance, quantity of information are abundant, easily intellectuality, long service life, the exclusive characteristics such as with low cost, fast and convenient.From multifrequency staphylococcus aureus, the Escherichia coli O 157 in the pulse sensor-based system monitoring liquid nutrient culture media significantly: the result of study of H7 and bacillus subtilis growing state shows that multifrequency significantly pulse sensor-based system is having very large application prospect aspect monitoring, analysis, differentiation and the fast detecting food-borne pathogenic microorganism.
Certainly, those of ordinary skill in the art will be appreciated that, above embodiment illustrates the present invention, and be not as limitation of the invention, as long as in essential scope of the present invention, all will drop in the scope of claims of the present invention variation, the modification of the above embodiment.
Claims (1)
1. the detection method of labelling foodstuff pathogenic bacteria growth, described food pathogenic is staphylococcus aureus; It is characterized in that may further comprise the steps:
A. with the staphylococcus aureus bacterial classification inoculation in 1% glucose broth, static cultivation 18 hours, the preparation seed liquor;
B. with seed liquor respectively equivalent transfer in the triangular flask that 50ml 1% glucose broth is housed, after cultivating respectively 0h, 1h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, 12 h, 13 h, 24 h, 30 h, to 100 ℃ of nutrient solutions, 40min water-bath deactivation;
C. with multifrequency significantly the nutrient solution of pulse intelligent chemical analysis system after to deactivation detect; It is working electrode that sensor array adopts tungsten electrode, platinum electrode, titanium electrode, silver electrode, and platinum electrode is as auxiliary electrode, and Ag/AgCl is as contrast electrode; The multifrequency significantly pulsed frequency of pulse excitation voltage is comprised of 1 Hz, 10 Hz and three pulsed frequencies of 100 Hz, and being equivalent to respectively pulse interval is the significantly pulse voltammetry of 1 s, 0.1 s, 0.01 s; The pulse height of the significantly pulse of described three pulsed frequencies all adopts the identical pulse changes in amplitude, since 1.0 V, then changes 0.2V until-1.0 V at every turn; Between two of described three pulsed frequencies different frequencies, insert the 5s time interval;
D. with multifrequency significantly the sensor array of pulse intelligent chemical analysis system put into and cultivate behind 0h, 1h, 2 h, 3 h, 4 h, 5 h, 6 h, 7 h, 8 h, 9 h, 10 h, 11 h, 12 h, 13 h, 24 h, 30 h and the nutrient solution of deactivation, apply voltage at working electrode, each working electrode in the sensor array is carried out the pulsatile once sequence; The frequency of described pulse train is 1 Hz, 10 Hz and 100 Hz;
E. with principal component analytical method to multifrequency significantly the data of pulse intelligent chemical analysis system collection process, the summit of extraction current acquisition signal and flex point value are as the variable of test sample, shot chart by the principal component analytical method acquisition, different test sample is distinguished, identified, determine that tungsten electrode 100Hz frequency, platinum electrode 1Hz frequency, silver electrode 10Hz frequency, titanium electrode 10Hz frequency are as the monitoring system of monitoring the staphylococcus aureus growth situation.
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| CN105334405B (en) * | 2014-07-07 | 2019-04-09 | 北京大学深圳研究生院 | A kind of electrical characteristic detector of liquid beverage |
| CN108531542B (en) | 2018-03-19 | 2021-11-26 | 中国水产科学研究院黄海水产研究所 | Automatic drug sensitivity test device for gram-negative bacteria and application method |
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