CN109295157A - A kind of Brazil's aspergillus is used for the purposes and method of cigarette mould inhibitor fungistatic effect indicator bacteria - Google Patents
A kind of Brazil's aspergillus is used for the purposes and method of cigarette mould inhibitor fungistatic effect indicator bacteria Download PDFInfo
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- CN109295157A CN109295157A CN201811245974.0A CN201811245974A CN109295157A CN 109295157 A CN109295157 A CN 109295157A CN 201811245974 A CN201811245974 A CN 201811245974A CN 109295157 A CN109295157 A CN 109295157A
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Abstract
Purposes and method the invention discloses a kind of Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria, the Brazil's aspergillus indicator bacteria ATCC reference culture number is 16404.Method of the Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria includes the following steps: that (1) activates Brazil's aspergillus；(2) Brazil's aspergillus is cultivated；(3) Brazil's aspergillus spore suspension is prepared；(4) plate containing bacterium is prepared；(5) sterile Oxford cup is placed；(6) mould inhibitor dilution is prepared；(7) mould inhibitor is added in Oxford cup；(8) culture experiment group and control group；(9) antibacterial circle diameter is measured with crossing method, records data；(10) susceptibility is divided according to average diameter of inhibition zone.Method of the invention solve the problems, such as the screening of cigarette mould inhibitor fungistatic effect indicator bacteria it is cumbersome, there may be security risk, have many advantages, such as that short, highly-safe, the representative strong and accuracy of cultivation cycle is high.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of Brazil's aspergillus refers to for cigarette mould inhibitor fungistatic effect
Show the purposes and method of bacterium.
Background technique
China is that the first leaf tobacco production big country, yield account for about the one third of Gross World Product in the world.Tobacco business is
One of the important sources of one of mainstay industry substituted and state revenue are difficult in CHINESE INDUSTRIES.Along with me
The good situation that the quickening of state's Cigarette Industrial Enterprise union and recombination and structure are persistently promoted, Cigarette Industrial Enterprise is to High Quality Tobacco
Demand is more urgent.But in cigarette sale and storage, influenced by ambient humidity, it is easy to happen mildew phenomena, from
And causes cigarette quality to reduce and even scrap.Therefore, prevention and treatment tobacco mildew is the important content of cigarette comprehensive treatment, and cigarette is mould proof
The research and development of agent are also important link needed for improving cigarette quality.During developing cigarette mould inhibitor, need one kind can
Accurate evaluation mould inhibitor to the indicator bacteria of cigarette mould inhibitory effect, with develop have targetedly new and effective cigarette it is mould proof
Agent, instead of traditional broad-spectrum antibacterial chemical anti mildew agent.The indicator bacteria should simultaneously with high security, indicative function it is good,
Cultivation cycle is short, is easy to the features such as experimental implementation.
Indicator bacteria used in mould inhibitor R&D process at present, the mostly mildew micro-organism comprising multiple categories or its mixing
Bacterium, such as contain aspergillus, Penicillium, mucor, the spore suspension of rhizopus or mixing spore suspension.Such method need to be from mould
It is easy when becoming in cigarette and screen isolated more plants of mildew micro-organisms, but separation process time and effort consuming, and making spore suspension
Existing operating error, is difficult to compare between not belonging to, and is not easy to control variable, influences the accuracy of experimental data.In addition, there is document will
As mould inhibitor fungistatic effect indicator bacteria, but mostly, the metabolite of fungi is that mycotoxin is such as yellow bent to the single plants such as aspergillus flavus fungi
Mould toxin etc. may have carcinogenic, teratogenesis and mutagenesis, and largely contact seriously threatens experimenter's in experimental implementation
Health and Environmental security.Above-mentioned indicator bacteria is unsuitable for the purposes for cigarette mould inhibitor fungistatic effect indicator bacteria.Therefore urgent
Need efficient one kind, safety, representative strong and high accuracy cigarette mould inhibitor fungistatic effect indicator bacteria.
To solve the above problems, proposing the present invention.
Summary of the invention
Brazil's aspergillus (Aspergillus brasiliensis) is and aspergillus niger (Aspergillus niger) high homology
Property close relative's bacterial strain, although at present there has been no numerous studies report, its close relative's bacterial strain aspergillus niger be by U.S. FDA authenticate peace
Full strain (GRAS), aspergillus niger is because not generate toxin, cultivation cycle short for it, frequently as fermentation strain for making sauce, soy sauce
With rice wine etc..
The present invention filters out cigarette and causes the strongest one plant of bacterium of mouldiness by the cause mouldiness of 15 kinds of cigarette mildew micro-organisms of measurement
Strain Brazil's aspergillus, is used as the indicator bacteria of cigarette mould inhibitor fungistatic effect.A kind of method are as follows: by designing simulation cigarette ingredient
The model media of nutrient environment, the cigarette for measuring every plant of mildew micro-organism cause mouldiness, filter out and cause mouldiness the most outstanding one
Indicator bacteria of the strain mildew micro-organism Brazil's aspergillus as cigarette mould inhibitor fungistatic effect.By measuring the mould inhibitor under a certain concentration
The mould inhibitor under fungistatic effect and the same concentrations to Brazil's aspergillus indicator bacteria spore suspension is to 15 kinds of micro- lifes of cigarette mildew
The fungistatic effect of object spore suspension.The results show that the cause mouldiness of Brazil's aspergillus is most strong, and there are other cigarette mildew micro-organisms
Similar suppressed characteristic, can be used for the indicator bacteria of cigarette mould inhibitor fungistatic effect.It is obtained by 18SrDNA-ITS sequencing
Know, Brazil's aspergillus used and ATCC16404 reference culture are bacterial strain of the same race, can be with during development of new cigarette mould inhibitor
Directly commercially available ATCC16404 reference culture Brazil's aspergillus is used as the indicator bacteria of cigarette mould inhibitor fungistatic effect, solves current volume
The problem of screening of cigarette mould inhibitor fungistatic effect indicator bacteria is cumbersome, there may be security risks.
Brazil's aspergillus is used for the purposes of cigarette mould inhibitor fungistatic effect indicator bacteria by the present invention, and specific method is using Oxford
Agar diffusion method measures cigarette mould inhibitor inhibition zone to evaluate its fungistatic effect.
Technical scheme is as follows:
First aspect present invention discloses a kind of purposes of Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria, institute
Stating Brazil's aspergillus indicator bacteria ATCC reference culture number is 16404.
Second aspect of the present invention discloses a kind of method of Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria, packet
Include following steps:
(1) commercially available Brazil's aspergillus is activated under specified requirements；
(2) by the Brazil's aspergillus streak inoculation after activation in slant medium test tube, in static state constant-temperature constant humidity incubator
Middle culture, with 2 days for a cultivation cycle；
(3) Brazil's aspergillus spore suspension is prepared with the inclined-plane bacterium colony that step (2) culture obtains；
(4) it is trained using the preparation spore suspension of Brazil's aspergillus containing 1ml of Brazil's aspergillus spore suspension made from step (3) and 9ml
The plate containing bacterium for supporting base rocks uniform and cooling；
(5) 3 sterile Oxford cups are uniformly put into plate containing bacterium；
(6) mould inhibitor is diluted step by step by 0.45 μm of pin type membrane filtration, obtains certain density mould proof dilution agent
Liquid；
(7) the mould inhibitor dilution that step (6) obtains is added in the Oxford cup of step (5), each Oxford cup adds 200 μ
L, with equivalent with concentration mould inhibitor dilution solvent as positive controls；
(8) experimental group of step (7) and positive controls are put into constant temperature and humidity incubator and are cultivated, with 1 day for one
Cultivation cycle；
(9) antibacterial circle diameter is measured with crossing method, records data；
(10) evaluation method: it is insensitive (-) that average diameter of inhibition zone, which is less than 8mm, and 8-10mm is less sensitive (+), 10-
15mm is medium sensitivity (++), and the above are highly sensitive (+++) by 15mm.
Preferably, culture medium prescription described in step (2) or (4) includes following ingredient: dehydrated potato powder 6.0g/L；Glucose
20.0g/L；Chloramphenicol 0.1g/L；Agar 20.0g/L.
Preferably, culture parameters described in step (2) or (8) are 28 DEG C, 85%RH.
Preferably, step (2) the Brazil's aspergillus spore suspension with 85mg/ml sterile saline the preparation method comprises the following steps: rushed
The inclined-plane culture primary surface containing bacterium colony is brushed, whirlpool concussion 30s obtains spore suspension；After 4 layers of sterile gauze filtering twice, pass through
Blood counting chamber counts, and is diluted to 1 × 105CFU/ml obtains the Brazil's aspergillus spore suspension.
Preferably, the Oxford cup specification is 8*6*10mm.
Preferably, mould inhibitor described in step (6) is the one or more of cinnamic acid, eugenol or citral.
Beneficial effects of the present invention:
1, the present invention is for the first time using Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria.Brazil's aspergillus causes mouldiness
By force, there is the similar suppressed characteristic of other cigarette mildew micro-organisms, it is micro- can accurately to indicate that cigarette mould inhibitor goes mouldy to cigarette
The inhibitory effect of biology, representative strong, accuracy height.
2, method of the invention solves cigarette mould inhibitor fungistatic effect indicator bacteria in cigarette mould inhibitor development process and screens
Cumbersome problem, can be directly using commercially available gained Brazil's aspergillus as mould inhibitor fungistatic effect indicator bacteria, it may not be necessary to carry out again
Heavy indicator bacteria screening operation.
3, method of the invention shortens the cultivation cycle of cigarette mould inhibitor fungistatic effect experiment, only needs 1 day, saves reality
Cost is tested, the R&D cycle is accelerated.And other are used to evaluate the indicator bacteria of mould inhibitor fungistatic effect, such as aspergillus flavus, mould, Mucor
Deng cultivation cycle generally at least need 5 days.
4, when method of the invention solves selection cigarette mildew micro-organism as mould inhibitor fungistatic effect indicator bacteria, fungi
The problem of secondary metabolite toxin may threaten to human health, it is highly-safe.
5, method of the invention simplifies bacteriostatic experiment operating procedure, reduces the experimental implementation error being likely to occur, mentions
High experimental data accuracy.
Specific embodiment
For a better understanding of the present invention, the content of invention is furtherd elucidate below with reference to embodiment, but of the invention interior
Appearance is not limited solely to the following examples.
Embodiment 1: mildew micro-organism causes mould performance test.
With tobacco curing juice culture medium, the method is as follows: 50.0g tobacco leaf is taken, 1000mL distilled water is added and boils, 2 layers of filtered through gauze
After twice, 18.0g agar is added, is settled to 1000mL, 121 DEG C of high pressure sterilization 15min, when being cooled to 50 DEG C or so, every 200ml training
Support base in be added 20mg chloramphenicol, rock it is uniform and cooling it is spare, obtain cigarette juice culture medium flat plate.Picking is stored in inclined-plane culture
The colony edge spore of 15 kinds of mildew separating obtained cigarette mildew micro-organisms of cigarette on base, point are planted in above-mentioned cigarette juice culture medium
The heart is inverted in 28 DEG C, cultivates 5 days in the constant temperature and humidity incubator of 85%RH condition of culture, straight with crossing method measurement bacterium colony
Diameter, every group is done 3 and is averaged in parallel, plants the cigarette juice culture medium flat plate handled as negative control group not put.As a result table
Bright, it is Brazil's aspergillus (Aspergillus respectively that the strongest 3 plants of strains of mouldiness energy are caused in 15 kinds of cigarette mildew micro-organisms
Brasiliensis), the Alternaria mould that aspergillus flavus (Aspergillus flavus) and certain plant of non-precise Identification go out, bacterium colony
Diameter is respectively 75.67 ± 0.89mm, 56.92 ± 1.09mm and 41.50 ± 1.00mm.Wherein, the cause mouldiness energy of Brazil's aspergillus
It is most strong, there is good representativeness.It is learnt by 18SrDNA-ITS sequencing, the Brazil's aspergillus (Aspergillus
It brasiliensis is) bacterial strain of the same race with commercially available ATCC16404 reference culture.
Embodiment 2: method of the Brazil's aspergillus as cigarette mould inhibitor cinnamic acid fungistatic effect indicator bacteria
By commercially available ATCC16404 reference culture under specified requirements after (condition is provided by businessman) activation, streak inoculation
In slant medium test tube, cultivated 2 days in 28 DEG C, the static state constant-temperature constant humidity incubator of 85%RH condition of culture；Culture medium
Formula includes following ingredient: dehydrated potato powder 6.0g/L；Glucose 20.0g/L；Chloramphenicol 0.1g/L；Agar 20.0g/L.By oblique
Bacterium colony obtained by the culture medium culture of face prepares Brazil's aspergillus spore suspension: being washed away with 85mg/ml sterile saline containing bacterium colony
Inclined-plane culture primary surface, whirlpool concussion 30s obtain spore suspension；After 4 layers of sterile gauze filtering twice, pass through blood counting chamber
It counts, is diluted to 1 × 105CFU/ml.By spore suspension preparation method of the same race, it is separating obtained to prepare 15 kinds of mildew cigarette
The spore suspension of cigarette mildew micro-organism obtains cigarette mildew micro-organism Mixed Microbes spore suspension after mixed in equal amounts.By 1ml Brazil
Aspergillus spore suspension or 1ml cigarette mildew micro-organism Mixed Microbes spore suspension are added in sterile petri dish, mix with 9ml culture medium
It shakes up and cools down, plate containing bacterium is made, be uniformly put into 3 sterile Oxford cups in each plate containing bacterium.Cinnamic acid is passed through into 0.45 μ
Its concentration is used 95v/v% ethyl alcohol to be diluted to 2.50mg/ml as mould inhibitor fungistatic effect test sample by m pin type membrane filtration.
200 μ l cinnamic acid dilutions are added into above-mentioned each Oxford cup, positive controls are done with equivalent 95v/v% ethyl alcohol, every group is done 3
It is a parallel, it is put into 28 DEG C, cultivates 1 day in the static state constant-temperature constant humidity incubator of 85%RH condition of culture, measured with crossing method
Antibacterial circle diameter records data and is averaged.The result shows that 2.50mg/ml cinnamic acid acts on Brazil's aspergillus indicator bacteria
Inhibition zone be 23.15 ± 0.96mm, act on cigarette mildew Mixed Microbes inhibition zone be 27.14 ± 0.74mm.Illustrate Brazil
Aspergillus and cigarette mildew Mixed Microbes are highly sensitive (+++) to cigarette mould inhibitor cinnamic acid.
Embodiment 3: method of the Brazil's aspergillus as cigarette mould inhibitor eugenol fungistatic effect indicator bacteria
According to the method for embodiment 2, cigarette mould inhibitor is replaced with into isoconcentration eugenol by cinnamic acid.The result shows that
The inhibition zone that 2.50mg/ml eugenol acts on Brazil's aspergillus indicator bacteria is 22.03 ± 0.82mm, acts on cigarette mildew mixing
The inhibition zone of bacterium is 22.52 ± 0.52mm.Illustrate Brazil's aspergillus and cigarette mildew Mixed Microbes to cigarette mould inhibitor eugenol height
Sensitive (+++).
Embodiment 4: method of the Brazil's aspergillus as cigarette mould inhibitor citral fungistatic effect indicator bacteria
According to the method for embodiment 2, cigarette mould inhibitor is replaced with into isoconcentration citral by cinnamic acid.The result shows that
The inhibition zone that 2.50mg/ml citral acts on Brazil's aspergillus indicator bacteria is 10.74 ± 0.58mm, acts on cigarette mildew mixing
The inhibition zone of bacterium is 13.87 ± 0.43.Illustrate that Brazil's aspergillus and cigarette mildew Mixed Microbes are quick to cigarette mould inhibitor citral moderate
Feel (++).
Embodiment described above shows that Brazil's aspergillus causes mouldiness relatively strong, and has 15 kinds of cigarette mildew micro-organisms similar
Suppressed characteristic.Use Odontothrips loti measurement inhibition zone mode evaluate several cigarette mould inhibitors act on Brazil's aspergillus, with
And the inhibition zone result sizes of 15 kinds of cigarette mildew Mixed Microbes are close, illustrate that Brazil's aspergillus can accurately indicate cigarette mould inhibitor pair
The inhibitory effect of cigarette mildew micro-organism；And because the cause mouldiness of isoconcentration Brazil's aspergillus is greater than 15 kinds of cigarette mildew Mixed Microbes
Mouldiness is caused, under the experiment condition of same concentration kinds of cigarettes mould inhibitor, the former inhibition zone is smaller than the latter.Therefore, Brazilian
Aspergillus can accurately indicate cigarette mould inhibitor to the inhibitory effect of cigarette mildew micro-organism, representative strong, accuracy height.
The foregoing is merely a prefered embodiment of the invention, is not intended to limit the invention, all in the spirit and principles in the present invention
Within any modification, equivalent substitution, improvement and etc. done, should be included within the scope of the present invention.
Claims (7)
1. a kind of purposes of Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria, which is characterized in that the Brazil's aspergillus
Indicator bacteria ATCC reference culture number is 16404.
2. a kind of method of Brazil's aspergillus as cigarette mould inhibitor fungistatic effect indicator bacteria, which comprises the steps of:
(1) commercially available Brazil's aspergillus is activated under specified requirements；
(2) it by the Brazil's aspergillus streak inoculation after activation in slant medium test tube, is trained in static state constant-temperature constant humidity incubator
It supports, with 2 days for a cultivation cycle；
(3) Brazil's aspergillus spore suspension is prepared with the inclined-plane bacterium colony that step (2) culture obtains；
(4) the preparation spore suspension of Brazil's aspergillus containing 1ml of Brazil's aspergillus spore suspension made from step (3) and 9ml culture medium are utilized
Plate containing bacterium, rock uniform and cooling；
(5) 3 sterile Oxford cups are uniformly put into plate containing bacterium；
(6) mould inhibitor is diluted step by step by 0.45 μm of pin type membrane filtration, obtains certain density mould inhibitor dilution；
(7) the mould inhibitor dilution that step (6) obtains being added in the Oxford cup of step (5), each Oxford cup adds 200 μ l, with
Equivalent is with concentration mould inhibitor dilution solvent as positive controls；
(8) experimental group of step (7) and positive controls are put into constant temperature and humidity incubator and are cultivated, be a culture with 1 day
Period；
(9) antibacterial circle diameter is measured with crossing method, records data；
(10) evaluation method: it is insensitive (-) that average diameter of inhibition zone, which is less than 8mm, and 8-10mm is less sensitive (+), 10-15mm
For medium sensitivity (++), the above are highly sensitive (+++) by 15mm.
3. according to the method described in claim 2, it is characterized in that, culture medium prescription described in step (2) or (4) includes as follows
Ingredient: dehydrated potato powder 6.0g/L；Glucose 20.0g/L；Chloramphenicol 0.1g/L；Agar 20.0g/L.
4. according to the method described in claim 2, it is characterized in that, culture parameters described in step (2) or (8) be 28 DEG C,
85%RH.
5. according to the method described in claim 2, it is characterized in that, step (2) the Brazil's aspergillus spore suspension preparation method
Are as follows: the inclined-plane culture primary surface containing bacterium colony is washed away with 85mg/ml sterile saline, whirlpool concussion 30s obtains spore suspension；Through
After 4 layers of sterile gauze filtering twice, is counted by blood counting chamber, be diluted to 1 × 105CFU/ml obtains the Brazil's aspergillus
Spore suspension.
6. according to the method described in claim 2, it is characterized in that, the Oxford cup specification is 8*6*10mm.
7. according to the method described in claim 2, it is characterized in that, mould inhibitor described in step (6) be cinnamic acid, eugenol or
The one or more of citral.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110031594A (en) * | 2019-05-24 | 2019-07-19 | 福建中烟工业有限责任公司 | Cigarette microorganism causes mouldiness detection method and detection device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106536747A (en) * | 2014-05-22 | 2017-03-22 | 纽勤有限公司 | Automated preservative efficacy test method and device |
| WO2017146036A1 (en) * | 2016-02-22 | 2017-08-31 | 参天製薬株式会社 | Pharmaceutical composition including dorzolamide and brimonidine |
| CN107541475A (en) * | 2017-04-25 | 2018-01-05 | 云南中烟工业有限责任公司 | One breeding oxen streptomycete and its application |
-
2018
- 2018-10-24 CN CN201811245974.0A patent/CN109295157A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106536747A (en) * | 2014-05-22 | 2017-03-22 | 纽勤有限公司 | Automated preservative efficacy test method and device |
| WO2017146036A1 (en) * | 2016-02-22 | 2017-08-31 | 参天製薬株式会社 | Pharmaceutical composition including dorzolamide and brimonidine |
| CN107541475A (en) * | 2017-04-25 | 2018-01-05 | 云南中烟工业有限责任公司 | One breeding oxen streptomycete and its application |
Non-Patent Citations (13)
| Title |
|---|
| 《化学百科全书》编辑委员会: "《化学百科全书（第18卷）》", 30 September 1998, 化学工业出版社 * |
| ANNA KOZIRÓG 等: ""Impact of Cationic and Neutral Gemini Surfactants on Conidia and Hyphal Forms of Aspergillus brasiliensis"", 《INT. J. MOL. SCI.》 * |
| D.J.BHUYAN 等: ""Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves"", 《SOUTH AFRICAN JOURNAL OF BOTANY》 * |
| NURHIDAYAH HAMZAH 等: ""Effect of surfactants on Aspergillus brasiliensis ATCC 16404 physicochemical properties"", 《JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING》 * |
| 姚粟 等: ""标准菌株ATCC 16404的分类学进展及影响"", 《食品与发酵工业》 * |
| 孟昭赫: "《食品卫生检验方法注解 微生物学部分》", 30 June 1990, 人民卫生出版社 * |
| 张成省 等: ""山东仓储片烟表面微生物区系研究"", 《中国烟草学报》 * |
| 朱桂宁 等: ""7种防霉剂对烟仓霉变微生物的抑制作用及防霉效果的初步研究"", 《广西农业生物科学》 * |
| 杨建卿 等: ""5种抑霉剂对储藏片烟霉菌的抑制效果"", 《安徽农业大学学报》 * |
| 许萍 等: ""壳聚糖对烟草抗菌活性研究"", 《化学工程师》 * |
| 谭才邓 等: ""抑菌试验中抑菌圈法的比较研究"", 《食品工业》 * |
| 赵文姬: ""云南仓储烟叶霉变及其生物防治研究"", 《中国优秀硕士学位论文全文数据库(电子期刊) 农业科技辑》 * |
| 黄福新 等: ""广西烟仓霉变发生状况调查及主要霉变因素探讨"", 《广西农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110031594A (en) * | 2019-05-24 | 2019-07-19 | 福建中烟工业有限责任公司 | Cigarette microorganism causes mouldiness detection method and detection device |
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Application publication date: 20190201 |