CN101337035A - Traditional Chinese preparation with bacteriostatic action for preventing blood sugar rising and preparation method thereof - Google Patents
Traditional Chinese preparation with bacteriostatic action for preventing blood sugar rising and preparation method thereof Download PDFInfo
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- CN101337035A CN101337035A CNA2008100737262A CN200810073726A CN101337035A CN 101337035 A CN101337035 A CN 101337035A CN A2008100737262 A CNA2008100737262 A CN A2008100737262A CN 200810073726 A CN200810073726 A CN 200810073726A CN 101337035 A CN101337035 A CN 101337035A
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- caulis sacchari
- sacchari sinensis
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Abstract
The invention relates to a traditional Chinese medicine preparation for blood glucose elevation inhibition and bacteriostasis action, as well as the preparation method and the application thereof. The traditional Chinese medicine preparation is prepared by extracting and processing sugarcane top and by adopting the conventional method, and is applicable to blood glucose elevation inhibition of the diabetics and bacterial infection treatment.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, particularly relate to a kind of Chinese medicine preparation and preparation method thereof and its purposes with inhibition blood sugar increasing, bacteriostasis.
Background technology:
Leaf of Caulis Sacchari sinensis is the leaf of Caulis Sacchari sinensis Saccharum sinensis Roxb..The medical material Caulis Sacchari sinensis is the cane of grass, extensively plants in the temperate zone and the torrid areas, and all there is cultivation each province, China south.The traditional Chinese medical science thinks that the Caulis Sacchari sinensis sweet in the mouth is cool in nature, clearing away heat and promoting production of body fluid is arranged, moisturize effect such as relieve the effect of alcohol, and can be used for diseases such as calentura Tianjin wound, restlessness and thirst, regurgitation vomiting, xeropulmonary cough, constipation due to dry stool.Leaf of Caulis Sacchari sinensis is the side-product behind the harvesting sugarcane, except that containing a large amount of chlorophylls, also contains number of chemical compositions such as vitamin C, equisetic acid, Caulis Sacchari sinensis polysaccharide.Leaf of Caulis Sacchari sinensis output is very big, each province, annual China south leaf of Caulis Sacchari sinensis output is up to tens million of tons, but so a large amount of leaf of Caulis Sacchari sinensis and being underutilized, except that small part as the animal feed, most of by sugarcane grower's on-site incineration, cause the wasting of resources and serious environment pollution.Though aspect rationally the utilizing of leaf of Caulis Sacchari sinensis, done some research work and obtained certain achievement in recent years, do not seen its report aspect medicinal.The inventor explores the application of leaf of Caulis Sacchari sinensis at field of medicaments in order to attempt to excavate the medical value of leaf of Caulis Sacchari sinensis, and having carried out is the research and development research work of the Chinese medicine preparation of main material with the leaf of Caulis Sacchari sinensis.This further promotes the Caulis Sacchari sinensis industry development to have positive effect for the comprehensive utilization and the deep development of Caulis Sacchari sinensis resource.
Summary of the invention
The objective of the invention is from leaf of Caulis Sacchari sinensis, to extract active substance, and the Chinese medicine preparation raw material of and inhibition diabetes blood glucose rising antibacterial as preparation with these active substances, make Chinese medicine preparation and be applied to treat bacterial infection, inhibition diabetes blood glucose rising, thereby enlarge the development of Caulis Sacchari sinensis industry, utilize the crop genetic resource of China better, enrich the scope of Chinese herbal medicine.
The inventor finds that the extract of leaf of Caulis Sacchari sinensis has tangible bacteriostasis, and for blood sugar increasing inhibitory action is arranged by the research to leaf of Caulis Sacchari sinensis, in experiment in vitro, obtained excellent curative.
Chinese medicine preparation of the present invention, its chemical identification is as follows:
(1) gets the Chinese medicine preparation sample for preparing under the preparation method of the present invention, water-soluble, filter, get filtrate 2ml, in test tube, add alkaline cupric tartrate reagent (Fehling) 4～5ml (face time spent first liquid and second liquid mixed in equal amounts are formed), put in the boiling water bath heating 5 minutes, take out, have red precipitate to produce.
(2) get the Chinese medicine preparation sample for preparing under the preparation method of the present invention, water-soluble, filter, get filtrate 1ml in test tube, add 2～3 of 5% alpha-Naphthol reagent, shake up, other gets a dried test tube, add concentrated sulphuric acid 1ml, slowly add above-mentioned biased sample liquid along tube wall, two liquid interfaces are purplish red colour circle.
The extract that the inventor extracts from leaf of Caulis Sacchari sinensis has been made preparation by the preparation method of Chinese medicine compound preparation, is used to suppress blood sugar increasing, treatment bacterial infection.In pharmacological research, said preparation has stronger specific aim, obtains preliminary effect.The present invention simultaneously also provides the Preparation method and use of clinical suitable dosage form.
Experimental study shows: leaf of Caulis Sacchari sinensis extract of the present invention all has effect external to staphylococcus aureus, escherichia coli, wherein playing a role clearly to staphylococcus aureus; The big or middle dosage group of leaf of Caulis Sacchari sinensis extract (comparing with model group) has certain inhibitory action to the rising of mouse blood sugar.Conclusion: the leaf of Caulis Sacchari sinensis extract has tangible antibacterial action; The inductive diabetic mice of alloxan is not had tangible blood sugar reducing function, but mouse blood sugar is had certain inhibitory action.
Below be the preparation method of the clinical suitable preparation of the present invention:
1, preparation method:
(1) get leaf of Caulis Sacchari sinensis, extracting in water 2-4 time filters, and merges the water extract, concentrates, and gets leaf of Caulis Sacchari sinensis extract cream, and formulation method adds adjuvant routinely, makes Chinese medicine preparation.
(2) get leaf of Caulis Sacchari sinensis, add alcohol reflux and extract 2-4 time, filtration merges alcohol extract, concentrates and reclaims alcohol, gets leaf of Caulis Sacchari sinensis extract cream, and formulation method adds adjuvant routinely, makes Chinese medicine preparation.
(3) get leaf of Caulis Sacchari sinensis, extracting in water 2-4 time filters, merge the water extract, being concentrated into relative density is 1.05-1.15, adds the ethanol of 80%-95% in the concentrated solution, contain alcohol to medicinal liquid and measure 55%-70%, left standstill 12-72 hour, and filtered, filtrate recycling ethanol is to there not being the alcohol flavor, concentrate, get leaf of Caulis Sacchari sinensis extract cream, formulation method adds adjuvant routinely, makes Chinese medicine preparation.
The explanation of preparation method:
1) in the above-mentioned preparation method, amount of water or add pure consumption be leaf of Caulis Sacchari sinensis weight 6-12 doubly
2) alcohol in the preparation method (2) comprises ethanol, methanol, propanol, n-butyl alcohol for the used alcohol agent of conventional extraction.
3) the leaf of Caulis Sacchari sinensis cream after above-mentioned preparation method concentrates can be prepared into the clear paste that relative density is 1.20-1.35, and being used for routinely, formulation method prepares liquid preparation; Also the needs of formulation method routinely are dried to dried cream, and it is standby to beat powder, is used to make solid preparation.
2, some dosage form preparation method embodiment of Chinese medicine preparation:
Embodiment 1.
Raw material: leaf of Caulis Sacchari sinensis.
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract 4 times, each 6 times of water gagings filter, and merge the water extract, are condensed into the clear paste that relative density is 1.20-1.35, and 80 ℃ of drying under reduced pressure are pulverized, and add right amount of auxiliary materials, make tablet.
Embodiment 2.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract 2 times, each 12 times of water gagings filter, and merge the water extract, are condensed into the clear paste that relative density is 1.20-1.35, and drying is pulverized, and adds right amount of auxiliary materials, makes granule.
Embodiment 3.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: leaf of Caulis Sacchari sinensis is ground into coarse powder, decocts with water to extract 3 times, and each 8 times of water gagings filter, and merge the water extract, are condensed into the clear paste that relative density is 1.20-1.35, and drying is pulverized, and adds right amount of auxiliary materials, makes powder.
Embodiment 4.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, add alcohol reflux 2 times, each 8 times of amount ethanol, filter, merge alcohol extract, reclaim ethanol and be condensed into the clear paste that relative density is 1.2-1.35 (60 ℃ of surveys), behind glyceryl monostearate heating and melting mixing, splash in the not miscible condensed fluid, shrink condensation and make sphere or the spheric preparation of class, packing promptly gets drop pill.
Embodiment 5.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract each 6 times of water gagings 4 times, filter, merge the water extract, be condensed into the clear paste that relative density is 1.05-1.15, add 95% ethanol and transfer to and contain alcohol amount 70%, stir, left standstill 12 hours, filter, decompression filtrate recycling ethanol, and the simmer down to relative density is the clear paste (60 ℃) of 1.2-1.35,80 ℃ of drying under reduced pressure are pulverized, and add adjuvant, mixing, briquetting or adorn tea bag or fry in shallow oil in the bag then, packing promptly gets medicinal tea.
Embodiment 6.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: leaf of Caulis Sacchari sinensis is ground into coarse powder, decocts with water to extract each 8 times of water gagings 2 times, filter, merge the water extract, be condensed into the clear paste that relative density is 1.05-1.15, add 95% ethanol and transfer to and contain alcohol amount 60%, stir, left standstill 72 hours, filter, decompression filtrate recycling ethanol, and the simmer down to relative density is the clear paste (60 ℃) of 1.2-1.35, adds refined honey and receives cream, add appropriate amount of auxiliary materials, packing promptly gets soft extract.
Embodiment 7.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract 8 times of amount of water, 6 times 2 times, filter, merge the water extract, be condensed into the clear paste that relative density is 1.05-1.15, add 85% ethanol and transfer to and contain alcohol amount 55%, stir, left standstill 48 hours, filter, decompression filtrate recycling ethanol, and the simmer down to relative density is the clear paste (60 ℃) of 1.2-1.35,80 ℃ of drying under reduced pressure are pulverized, and add right amount of auxiliary materials, mixing is divided in Capsules or is sealed in the soft capsule material, and packing promptly gets hard capsule product or soft capsule product.
Embodiment 9.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract each 8 times of water gagings 2 times, filter, merge the water extract, be condensed into the clear paste that relative density is 1.05-1.15, add 90% ethanol and transfer to the alcohol amount 65% that contains, stir, left standstill 24 hours, filter, decompression filtrate recycling ethanol, and the simmer down to relative density is the clear paste (60 ℃) of 1.2-1.35, adds right amount of auxiliary materials, makes mixture.
Embodiment 10.
Raw material: leaf of Caulis Sacchari sinensis
Method for making: get leaf of Caulis Sacchari sinensis, decoct with water and extract each 8 times of water gagings 2 times, filter, merge the water extract, be condensed into the clear paste that relative density is 1.05-1.15, add 90% ethanol and transfer to the alcohol amount 60% that contains, stir, left standstill 36 hours, filter, decompression filtrate recycling ethanol, and the simmer down to relative density is the clear paste (60 ℃) of 1.2-1.35, adds right amount of auxiliary materials, makes syrup.
3, pharmacology embodiment
(1) leaf of Caulis Sacchari sinensis extract bacteriostatic test (liquid diluting method)
It is 9 pipes that leaf of Caulis Sacchari sinensis extract medicinal liquid is made a series of doubling dilutions with the MH broth bouillon, makes the MH broth bouillon contain concentration and is respectively 1000mg/ml, 250mg/ml, 125mg/ml, 62.5mg/ml, 31.25mg/ml, 15.625mg/ml, 7.8125mg/ml, 3.9063mg/ml.The 10th pipe does not add medicinal liquid and inoculates identical strain for the MH broth bouillon and oppose and look after.Each pipe adds 100 μ l bacterium liquid (107CFU/ml), and put 35 ℃ and cultivated 24 hours, be the minimal inhibitory concentration (MIC) of this medicine with the lowest concentration of drug that the naked eyes visible growth do not occur.The result shows that the leaf of Caulis Sacchari sinensis extract is respectively 1000mg/ml, MIC125mg/ml to the MIC of escherichia coli, staphylococcus aureus.
(2) the leaf of Caulis Sacchari sinensis extract suppresses blood sugar test
Getting normal Kunming kind is that mice is a collection of, body weight 20～25g, ♀ ♂ dual-purpose, after water 12h is can't help in fasting, 8% alloxan normal saline solution of preparation is newly carried out tail vein injection by the dosage of 65mg/kg, and behind the 72h, water 8h is can't help in fasting, detect the blood glucose value of fasting 12h, the mice that deletion does not cause model.Get qualified mice and be divided into 5 groups at random, 14 every group, i.e. model group (waiting capacity NS); Positive controls (metformin 750mg/kg); 3 dosage groups of leaf of Caulis Sacchari sinensis water extract high, medium and low (30,15,7.5g/kg).Other get with batch 14 of normal mouses as blank group (waiting capacity NS).Administration group ig administration, every day 1 time, continuous 21 days.After the administration respectively at detecting blood glucose value on the the 7th, 14,21 day.Water 8h is can't help in fasting before surveying blood glucose, and blood sampling was measured after then water 2h was can't help in the continuation fasting after the administration.Choose the hyperglycemia mice of fasting glucose between 13.05 ± 7.23mmol/L, capillary glass tube with 0.9～1.1mm internal diameter is got blood from mice socket of the eye venous plexus, put room temperature and treat blood coagulation, the centrifugal 10min of 3500r/min, separation of serum, get serum 10 μ l,, measure trap (OD value) in the 505nm place by the operating procedure and the method for glucose kit.The result shows that leaf of Caulis Sacchari sinensis extract mice ig administration do not have the effect of obvious blood sugar lowering to the diabetes model due to the alloxan, show relatively that with model group the big or middle dosage group of 1 all leaf of Caulis Sacchari sinensis mouse blood sugar raises slowly behind the medicine, especially heavy dose of group has than the obvious suppression effect blood sugar increasing.
Claims (6)
1, a kind of Chinese medicine preparation with inhibition blood sugar increasing, bacteriostasis is characterized in that: this Chinese medicine preparation active substance is to be refined by leaf of Caulis Sacchari sinensis to process.
2, as the production method of right 1 described Chinese medicine preparation, it is characterized in that: get leaf of Caulis Sacchari sinensis, extracting in water 2-4 time filters, and merges the water extract, concentrates, and gets leaf of Caulis Sacchari sinensis extract cream, and formulation method adds adjuvant routinely, makes Chinese medicine preparation.
3, as the production method of right 1 described Chinese medicine preparation, it is characterized in that: get leaf of Caulis Sacchari sinensis, add alcohol reflux and extract 2-4 time, filtration merges alcohol extract, reclaims alcohol, concentrates, and gets leaf of Caulis Sacchari sinensis extract cream, and formulation method adds adjuvant routinely, makes Chinese medicine preparation.
4, as the production method of right 1 described Chinese medicine preparation, it is characterized in that: get leaf of Caulis Sacchari sinensis, extracting in water 2-4 time, filter, merge the water extract, being concentrated into relative density is 1.05-1.15, the ethanol that adds 80%-95% in the concentrated solution contains alcohol to medicinal liquid and measures 55%-70%, leaves standstill 12-72 hour, filter, filtrate recycling ethanol does not extremely have the alcohol flavor, concentrates, and gets leaf of Caulis Sacchari sinensis extract cream, formulation method adds adjuvant routinely, makes Chinese medicine preparation.
5, the purposes of Chinese medicine preparation as claimed in claim 1 is characterized in that: the application of this Chinese medicine preparation when the treatment bacterial infection.
6, the purposes of Chinese medicine preparation as claimed in claim 1 is characterized in that: the application of this Chinese medicine preparation in suppressing diabetes blood glucose's rising.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100737262A CN101337035B (en) | 2008-08-08 | 2008-08-08 | Traditional Chinese preparation with bacteriostatic action for preventing blood sugar rising and preparation method thereof |
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| CN2008100737262A CN101337035B (en) | 2008-08-08 | 2008-08-08 | Traditional Chinese preparation with bacteriostatic action for preventing blood sugar rising and preparation method thereof |
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| CN101337035A true CN101337035A (en) | 2009-01-07 |
| CN101337035B CN101337035B (en) | 2011-10-12 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919391A (en) * | 2012-09-25 | 2013-02-13 | 安徽双园茶业有限公司 | Fragrance-improving needle-like black tea producing method |
| CN103007090A (en) * | 2013-01-16 | 2013-04-03 | 广西中医药大学 | Preparation and application of sugarcane leaf extractive for preventing and curing decsyed teeth and preparations |
| CN114306527A (en) * | 2021-12-31 | 2022-04-12 | 广西养利农业投资开发有限公司 | Compound broadleaf holly leaf traditional Chinese medicine composition and application thereof in preparing shampoo |
| CN114306526A (en) * | 2021-12-31 | 2022-04-12 | 广西养利农业投资开发有限公司 | Compound broadleaf holly leaf traditional Chinese medicine extract and application thereof in preparing shower gel or hand sanitizer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100467567C (en) * | 2006-01-13 | 2009-03-11 | 暨南大学 | Method fox extracting sugar-cane-leaf oxidation-resisting matter and use thereof |
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2008
- 2008-08-08 CN CN2008100737262A patent/CN101337035B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919391A (en) * | 2012-09-25 | 2013-02-13 | 安徽双园茶业有限公司 | Fragrance-improving needle-like black tea producing method |
| CN103007090A (en) * | 2013-01-16 | 2013-04-03 | 广西中医药大学 | Preparation and application of sugarcane leaf extractive for preventing and curing decsyed teeth and preparations |
| CN114306527A (en) * | 2021-12-31 | 2022-04-12 | 广西养利农业投资开发有限公司 | Compound broadleaf holly leaf traditional Chinese medicine composition and application thereof in preparing shampoo |
| CN114306526A (en) * | 2021-12-31 | 2022-04-12 | 广西养利农业投资开发有限公司 | Compound broadleaf holly leaf traditional Chinese medicine extract and application thereof in preparing shower gel or hand sanitizer |
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| CN101337035B (en) | 2011-10-12 |
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Granted publication date: 20111012 Termination date: 20210808 |