CN101328224B - Fructus choerospondiatis polysaccharide metal complexe and preparation thereof - Google Patents
Fructus choerospondiatis polysaccharide metal complexe and preparation thereof Download PDFInfo
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- CN101328224B CN101328224B CN2007101176241A CN200710117624A CN101328224B CN 101328224 B CN101328224 B CN 101328224B CN 2007101176241 A CN2007101176241 A CN 2007101176241A CN 200710117624 A CN200710117624 A CN 200710117624A CN 101328224 B CN101328224 B CN 101328224B
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- fructus choerospondiatis
- polysaccharide
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Abstract
The invention relates to a chemical product and a manufacturing methodthereof, namely Fructus Choerospondiatis polysaccharide metal complex and a preparation method thereof. Fructus Choerospondiatis is a conventional medicinal material of Mongolian medicine and has good curative effect on cardio-cerebrovascular diseases, and Fructus Choerospondiatis polysaccharide is a main active ingredient in the Fructus Choerospondiatis. Metal cations have important function of health care and particularly have good combination effect with polysaccharide in the human body. The method synthesizes the Fructus Choerospondiatis polysaccharide metal complex on the basis of preparation of the Fructus Choerospondiatis polysaccharide based on the understanding. As proved by experiments, the Fructus Choerospondiatis polysaccharide metal complex has good medicinal value, can be widely used in the manufacturing industry of various foodstuffs, medicines and health products, can obviously improve the medical care level of products, shorten the product volume, and reduce the toxic side effect, and has wide application prospect, thereby providing theoretical basis and technical support for development and utilization of the Fructus Choerospondiatis.
Description
Affiliated technical field
The present invention relates to the production method of a kind of chemical products and this product, i.e. fructus choerospondiatis polyose metal complexes and preparation method thereof.
Background technology
Fructus choerospondiatis is the dry mature fruit of Anacardiaceae plant Fructus Choerospondiatis, is a kind of Mongolian medicine's conventional crude drugs, and cardiovascular and cerebrovascular diseases is had better curative effect.At present, we have extracted the fructus choerospondiatis polysaccharide, and have proved that the fructus choerospondiatis polysaccharide is the important source of fructus choerospondiatis drug effect.Polysaccharide refers generally to the polymerization degree and exceeds saccharan more than 10, its molecular weight normally tens thousand of are class natural high moleculer eompounds to millions of, extensively be present in various animals, plant and the microorganism cultures.Research data proves that polysaccharide has higher pharmaceutical use and health-care effect, particularly the effect of pharmaceutical activity such as anticoagulation, antithrombotic.The intravital polysaccharide of people combines with metallic cations such as zinc, calcium, iron, selenium, is bringing into play important health-care effect., how the carrier of existing metallic cation supplement does not have nutritive value, and what have can't directly utilize, and need carry out complicated processes such as bio-transformation.Comprehensive above-mentioned information can be foretold, if the fructus choerospondiatis polysaccharide can be combined with metallics, will produce positive effect.
Summary of the invention
The objective of the invention is the fructus choerospondiatis polysaccharide is combined with the metallic cation material, obtain the fructus choerospondiatis polyose metal complexes, and propose to be suitable for industrial fructus choerospondiatis polyose metal complexes preparation method.
By a large amount of experiments, obtain the fructus choerospondiatis polyose metal complexes, its diffuse reflectance infrared spectroscopy peak data is 3423-3419 (OH), 2926-2847 (CH
2-), 1607-1621 (C=O), 1159-1132 (C-O-C), the 1098-1015 (charateristic avsorption band that β-pyranose and α-pyranose glycosidic bond C-O-H), is all arranged.
The preparation method of said fructus choerospondiatis polyose metal complexes is: polysaccharide dissolving back adds the polyose metal mixed solution, get filtrate after filtration, again filtrate dialysis is purified dialyzate, again dialyzate concentrate, alcohol is analysed, precipitate, washing, drying, obtains the pure product of polyose metal.
The preparation method of said fructus choerospondiatis polyose metal complexes is: get the fructus choerospondiatis polysaccharide, be dissolved in water, add the metal ion solution of 1mol/L.To between the 6-7,2-4 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialysis (dialysis tubing XH420, the scope of damming 8000--10000) is purified, with dialyzate concentrate, alcohol is analysed, precipitate, washing, drying, and the fructus choerospondiatis polyose metal material of certain color must be arranged.
The fructus choerospondiatis polyose metal complexes that adopts technique scheme to produce, can be widely used in the production of varieties of food items, medicine, healthcare products, can obviously improve the health care level of relevant product, dwindle small product size, reduce toxic side effect, demonstrate wide application prospect.
Description of drawings
Fig. 1 is the infrared spectrogram of fructus choerospondiatis Polyferose;
Fig. 2 is the infrared spectrogram of fructus choerospondiatis polysaccharide calcium;
Fig. 3 is the infrared spectrogram of fructus choerospondiatis polysaccharide selenium;
Fig. 4 is the infrared spectrogram of fructus choerospondiatis polysaccharide tungsten;
Fig. 5 is the preparation general formula block diagram of fructus choerospondiatis polyose metal.
Embodiment
First kind of embodiment: the reparation technology of fructus choerospondiatis Polyferose.
Get the fructus choerospondiatis polysaccharide, be dissolved in water, add the FeCl of 1mol/L
2Solution.To between the 6-7,4-6 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialyse (dialysis tubing XH420, the scope of damming 8000--10000) purifies, will dialyse concentrate, alcohol is analysed, precipitate, washing, drying, must be the fructus choerospondiatis Polyferose by the reddish-brown material.
The physicochemical character of products obtained therefrom: the fructus choerospondiatis Polyferose is the reddish-brown powder, do not have smell, tasteless, soluble in water, its aqueous solution is reddish brown red clear solution.Be insoluble to organic solvents such as anhydrous methanol, ethanol, ether.
The structure of product: the infrared spectrogram of fructus choerospondiatis Polyferose shown in Figure 1, shown the diffuse reflectance infrared spectroscopy peak of fructus choerospondiatis Polyferose, the characteristic peak data are 3420.83 (OH), 2853.22-2925.44 (CH
2-), 1620.28 (C=O), 1158.02 (C-O-C), 1068.14 (C-O-H), 902.97,859.41cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose and α-pyranose glycosidic bond.
Second kind of embodiment: the preparation method of fructus choerospondiatis polysaccharide calcium.
Get the fructus choerospondiatis polysaccharide, be dissolved in water, add the CaCl of 1mol/L
2Solution.To between the 6-7,4-6 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialyse (dialysis tubing XH420, the scope of damming 8000--10000) purifies, will dialyse concentrate, alcohol is analysed, precipitate, washing, drying, must be the fructus choerospondiatis Polyferose by the reddish-brown material.
The physicochemical character of products obtained therefrom: fructus choerospondiatis polysaccharide calcium is the red-brown powder, do not have smell, tasteless, soluble in water, its aqueous solution is reddish brown red clear solution.Be insoluble to organic solvents such as anhydrous methanol, ethanol, ether.
The structure of product: the infrared spectrogram of fructus choerospondiatis polysaccharide calcium shown in Figure 2, shown the diffuse reflectance infrared spectroscopy peak of fructus choerospondiatis polysaccharide calcium, the characteristic peak data are 3410.42 (OH), 2929.21 (CH
2-), 1614.10 (C=O), 1145.50 (C-O-C), 1079.13 (C-O-H), 894.32,859.41cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose glycosidic bond.
The third embodiment: the preparation method of fructus choerospondiatis polysaccharide selenium.
Get the fructus choerospondiatis polysaccharide, be dissolved in water, add the Na of 1mol/L
2SeO
3Solution.To between the 6-7,4-6 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialyse (dialysis tubing XH420, the scope of damming 8000--10000) purifies, will dialyse concentrate, alcohol is analysed, precipitate, washing, drying, must be fructus choerospondiatis polysaccharide selenium by the reddish-brown material.
The structure of product: the infrared spectrogram of fructus choerospondiatis polysaccharide selenium shown in Figure 3, shown the diffuse reflectance infrared spectroscopy peak of fructus choerospondiatis polysaccharide selenium, the characteristic peak data are 3422.74 (OH), 2924.81-2853.43 (CH
2-), 1608.66 (C=O), 1132.02 (C-O-C), and 1056.10--1016.67 (C-O-H), 951.75,881.45cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose and α-pyranose glycosidic bond.
The 4th kind of embodiment: the preparation method of fructus choerospondiatis polysaccharide tungsten.
Get the fructus choerospondiatis polysaccharide, be dissolved in water, add the sodium tungstate solution of 1mol/L.To between the 6-7,4-6 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialysis (dialysis tubing XH420, the scope of damming 8000--10000) is purified, with dialyzate concentrate, alcohol is analysed, precipitate, washing, drying, the reddish-brown material be fructus choerospondiatis polysaccharide tungsten.
The structure of product: the infrared spectrogram of fructus choerospondiatis polysaccharide tungsten shown in Figure 4 has shown the diffuse reflectance infrared spectroscopy peak of fructus choerospondiatis polysaccharide tungsten, and the characteristic peak data are 3422.17 (OH), 2925.98-2847.52 (CH
2-), 1613.44 (C=O), 1151.95 (C-O-C), and 1097.24-1015.82 (C-O-H), 962.89,894.32,811.99cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose and α-pyranose glycosidic bond.
Sum up the preparation method and the constructional feature of fructus choerospondiatis polyose metal iron, calcium, selenium, tungsten, zinc, magnesium, nickel, copper, cobalt etc., can obtain the preparation general formula of following fructus choerospondiatis polyose metal, general preparation method and constructional feature.
Fig. 5 has represented the preparation general formula of fructus choerospondiatis polyose metal, and the synthesis step of fructus choerospondiatis polyose metal is as seen from the figure: the fructus choerospondiatis polysaccharide is dissolved in water, mix with metal salt solution, and the low-grade fever reaction, cooling is filtered, and filtrate is concentrated alcohol analyse, and gets crude product.Again with dissolving crude product, dialysis is purified, and reconcentration, alcohol are analysed, precipitated, drying, the pure product of fructus choerospondiatis polyose metal complexes.
The general preparation method of fructus choerospondiatis polyose metal:
Get the fructus choerospondiatis polysaccharide, be dissolved in water, add the metal ion solution of 1mol/L.To between the 6-7,4-6 hour postcooling of low-grade fever reaction, filtration concentrate alcohol with filtrate and analyse with the adjusting PH with base value of 0.5mol/L, the brown material be crude product.Again with dissolving crude product, dialysis (dialysis tubing XH420, the scope of damming 8000--10000) is purified, with dialyzate concentrate, alcohol is analysed, precipitate, washing, drying, and the fructus choerospondiatis polyose metal material of certain color must be arranged.
Constructional feature: conclude above-mentioned fructus choerospondiatis polyose metal (iron, calcium, selenium, tungsten, zinc, magnesium, nickel, copper, cobalt etc.) infrared spectrogram, the diffuse reflectance infrared spectroscopy peak data that obtains the fructus choerospondiatis polyose metal is 3423-3419 (OH), 2926-2847 (CH
2-), 1607-1621 (C=O), 1159-1132 (C-O-C), the 1098-1015 (charateristic avsorption band that β-pyranose and α-pyranose glycosidic bond C-O-H), is all arranged.
The experiment overview:
The medical care effect of polysaccharide and metallic element is confirmed by a large amount of experiments and practical application, can infer fructus choerospondiatis polysaccharide and polyose metal complexes thereof fully according to existing data and have value medical health care.However, in order further to understand the special role for human body of fructus choerospondiatis polyose metal complexes, since 2005, we have carried out preliminary discussion to the medical care effect of fructus choerospondiatis polyose metal complexes.Observe by pharmacological evaluation, toxicological experiment and crowd, proved that the fructus choerospondiatis polysaccharide is under the prerequisite that keeps the basic property of medicine of fructus choerospondiatis, have more that produce effects is fast, strong drug action, dosage are little, be convenient to processing, be convenient to transportation, special advantages such as be convenient to take, and do not find any toxic side effect.In addition, we are also according to the index of recommending in " DRIs " of Chinese Soclety of Nutrition's establishment, nutrition feature to fructus choerospondiatis Polyferose, fructus choerospondiatis polysaccharide zinc, fructus choerospondiatis polysaccharide selenium, fructus choerospondiatis polysaccharide calcium has carried out experimentation on animals and crowd's observation, the oxidation-resistance of finding the fructus choerospondiatis polyose metal obviously is better than the fructus choerospondiatis polysaccharide, has proved that polysaccharide and metal interaction are more more remarkable to the influence that human body produces than single polysaccharide.
Discover that Polyferose, polysaccharide calcium and polysaccharide zinc all exist with solvable state in the pH=3-12 scope, promptly solubleness is very big, and this explanation Polyferose, polysaccharide calcium and polysaccharide zinc have bioavailability preferably.The control of metal content was relatively easy when also explanation prepared the fructus choerospondiatis polyose metal simultaneously, this point, for having special meaning with an amount of absorption of guaranteeing metallic element, the supplement that become supplement, particularly calcium, iron, zinc and the selenium of body trace element for the fructus choerospondiatis polyose metal provide condition.
Claims (5)
1. fructus choerospondiatis polyose metal complexes, it is characterized in that: the diffuse reflectance infrared spectroscopy peak data of said fructus choerospondiatis polyose metal complexes is 3423-3419 (OH), 2926-2847 (CH
2-), 1607-1621 (C=O), 1159-1132 (C-O-C), the 1098-1015 (charateristic avsorption band that β-pyranose and α-pyranose glycosidic bond C-O-H), is all arranged.
2. fructus choerospondiatis polyose metal complexes according to claim 1 is characterized in that: said fructus choerospondiatis polyose metal complexes comprises the fructus choerospondiatis Polyferose, and its diffuse reflectance infrared spectroscopy peak data is 3420.83 (OH), 2853.22-2925.44 (CH
2-), 1620.28 (C=O), 1158.02 (C-O-C), 1068.14 (C-O-H), 902.97,859.41cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose and α-pyranose glycosidic bond.
3. fructus choerospondiatis polyose metal complexes according to claim 1 is characterized in that: said fructus choerospondiatis polyose metal complexes comprises fructus choerospondiatis polysaccharide selenium, and its diffuse reflectance infrared spectroscopy peak data is 3422.74 (OH), 2924.81-2853.43 (CH
2-), 1608.66 (C=O), 1132.02 (C-O-C), and 1056.10-1016.67 (C-O-H), 951.75,881.45cm
-1Near absorption peak is respectively the charateristic avsorption band of β-pyranose and α-pyranose glycosidic bond.
4. fructus choerospondiatis polyose metal complexes according to claim 1, it is characterized in that: the preparation method of said fructus choerospondiatis polyose metal complexes is: fructus choerospondiatis polysaccharide dissolving back is added metal ion solution, get the polyose metal mixed solution, get filtrate after filtration, again filtrate dialysis is purified dialyzate, again dialyzate concentrate, alcohol is analysed, precipitate, washing, drying, obtains the pure product of polyose metal.
5. fructus choerospondiatis polyose metal complexes according to claim 4, it is characterized in that: the preparation method of said fructus choerospondiatis polyose metal complexes is: get the fructus choerospondiatis polysaccharide, be dissolved in water, the metal ion solution that adds 1mol/L, with the adjusting PH with base value of 0.5mol/L between the 6-7,2-4 hour postcooling of low-grade fever reaction, filter, filtrate is concentrated alcohol analyses, get the brown material and be crude product, with dissolving crude product, use the XH420 dialysis tubing again, dialysis is purified in the scope of damming of 8000-10000, dialyzate is concentrated, alcohol is analysed, precipitation, washing, drying obtains the fructus choerospondiatis polyose metal material of certain color.
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| CN102964459A (en) * | 2012-11-02 | 2013-03-13 | 华南理工大学 | Lentinan calcium complex, and preparation method and application thereof |
| CN105344313B (en) * | 2015-11-27 | 2018-01-16 | 内蒙古民族大学 | A kind of dissolved acetylene scarvenger |
| CN106629922A (en) * | 2017-01-07 | 2017-05-10 | 内蒙古民族大学 | Organics degrading agent and preparation method thereof |
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