CN101320820A - Application of enterobacter aerogenes in microbe power generation and its power generation method - Google Patents
Application of enterobacter aerogenes in microbe power generation and its power generation method Download PDFInfo
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- CN101320820A CN101320820A CNA2008100292220A CN200810029222A CN101320820A CN 101320820 A CN101320820 A CN 101320820A CN A2008100292220 A CNA2008100292220 A CN A2008100292220A CN 200810029222 A CN200810029222 A CN 200810029222A CN 101320820 A CN101320820 A CN 101320820A
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- clostridium perfringen
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- enterobacter aerogenes
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
- Y02E60/30—Hydrogen technology
- Y02E60/50—Fuel cells
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Abstract
The invention discloses application of enterobacter aerogenes in the aspect of microbe generation and power generating method. The enterobacter aerogenes can be applied to the aspect of microbe power generation as anode catalyst of microbe fuel battery. The power generating method provided by this invention at the same time includes following steps: constructing microbe fuel battery; preparing enterobacter aerogenes inoculum; preparing anode liquid containing fuel; adding the enterobacter aerogenes inoculum and prepared anode liquid into the anode chamber of the microbe fuel battery; performing power generation detection after static cultivation. The enterobacter aerogenes used in the invention belongs to the facultative anaerobe that is easy to be cultured and will not be deactivated under the existing condition of oxygen. The power generation activity can be maintained with low running cost for a long time by periodically adding culture medium. Meanwhile, the invention has wide fuel utilization range, complete biological oxidation and high electronic coefficient of recovery.
Description
Technical field
The invention belongs to environment and technical field of new energies, be specifically related to the application of clostridium perfringen aspect electricity generation by microorganism, is the electricity-generating method that anode catalyst produces electric energy with the clostridium perfringen.
Background technology
The energy exhausts that disposing with organic waste is two hang-ups that the world today faces.On the one hand, fossil fuel exhaustion is increasingly serious with the global warming problem, forces countries in the world to pay much attention to energy problem, and the research and development of renewable new forms of energy are imperative.Structure is stablized various energy resource system, has become the important component part of national strategy safety and sustainable development.On the other hand, how to handle the huge day by day organic waste of quantity, become serious social concern, the importance of organic waste resource or recovery energy also manifests day by day.
(Microbial Full Cell is to utilize the electrogenesis microbe chemical energy in the organic substance to be converted into the device of electric energy as anode catalyst MFC) to microbiological fuel cell.Compare with the conventional fuel battery, MFC replaces expensive chemical catalyst with microbe, has more advantages: 1) operating condition gentleness.Under normal temperature, normal pressure, move; 2) need not to preventing catalyst poisoning purification fuel; 3) environmental friendliness; 4) energy transformation ratio height; 5) good biocompatibility; 6) raw material sources are more extensive.In theory, all biodegradable organic substances all can be used as the fuel of MFC, and biodegradable organic substance includes organic waste water, human and animal excreta, rubbish from cooking or the like organic waste, and MFC can produce clean electric power when disposing debirs.
The electrogenesis microbe is as the anode catalyst of MFC, and oxidation of organic compounds and the speed of anode (being that anode is transmitted electronics) of reducing are the greatest factor that influences the MFC output power density.Therefore, the electrogenesis microbe is the core of MFC.At present, the electrogenesis microbe of having found has strain more than ten, mainly concentrates on Shiva Bordetella (Shewanella) and ground Bacillus (Geobacter).But as the MFC anode catalyst, there is certain defective in they: 1) belong to strict anaerobes, the existence of oxygen can make its inactivation or forfeiture electrogenesis activity, is unfavorable for bacterial strain large-scale culture and practical application; 2) the fuel utilization of most bacterial strains spectrum is narrower, can only utilize small-molecular weight organic acids such as acetate, lactic acid as electron donor (being fuel) electrogenesis.When acting as a fuel with carbohydrate or other complicated organic substance, need at first utilize zymogenic bacteria that it is degraded to simple organic acid, could electrogenesis.
Clostridium perfringen is the facultative anaerobe that occurring in nature extensively exists, and publication number is to disclose a strain in the CN101096645 application for a patent for invention to have iron reducing activity, amphimicrobian, clostridium perfringen that electron donor utilization spectrum is wider.Through document and patent retrieval, do not find to have the report of electrogenesis activity as yet about clostridium perfringen.
Summary of the invention
An object of the present invention is to overcome the deficiency that prior art exists, the application of clostridium perfringen aspect electricity generation by microorganism is provided.
Electricity-generating method when another object of the present invention provides described clostridium perfringen and is applied to electricity generation by microorganism.
Purpose of the present invention is achieved by the following technical programs:
Provide a kind of clostridium perfringen as the anode catalyst of microbiological fuel cell in the application aspect the electricity generation by microorganism.
Clostridium perfringen fuel utilization spectrum broad, amphimicrobian, it is characterized by: rod-short, size range is 1.0～1.7 * 0.6～0.8 μ m, bacterium colony is white in color or is yellow, amphimicrobian, can with the anode multiple organic substances such as electron acceptor, oxidation citric acid, glycerol, glucose, sucrose, starch under the anaerobic environment.Utilize it as the microbiological fuel cell that anode catalyst makes up, the electronics rate of recovery is up to 42～83%.This bacterial strain called after Enterbacter aerogenesXM02, in on March 12nd, 2007 in China Committee for Culture Collection of Microorganisms's common micro-organisms center preservation, be numbered: CGMCC 1969, have been open in the CN101096645 application for a patent for invention at publication number simultaneously.
The present invention provides the electrogenesis method of described clostridium perfringen when being applied to electricity generation by microorganism simultaneously, specifically may further comprise the steps:
(1) makes up microbiological fuel cell;
(2) preparation clostridium perfringen thalline is as the microbe fuel cell inoculation thing;
(3) preparation contains the anolyte of fuel;
(4) clostridium perfringen inoculum and the anolyte for preparing are added in the anode of microbial fuel cell chamber, leave standstill and carry out electrogenesis after the cultivation and detect.
The described preparation of step (2) clostridium perfringen somatic cells is to be seeded to the conventional LB fluid nutrient medium from the inclined-plane of preserving clostridium perfringen XM02, shaking table activation thalline is 16 hours under 30 ℃, 200 rev/mins conditions, reaching the exponential growth after date stops to cultivate, bacterium liquid was 4 ℃ of following 8000 rev/mins of high speed centrifugations 10 minutes, with 0.85% SPSS washing 2 times, be suspended in 0.85% SPSS again and prepare inoculum.
Described conventional LB Liquid Culture based component is: yeast extract 5g, and tryptone 10g, NaCl 5g, water 1000mL, pH 7.2～7.6.
The inoculation quantity of described clostridium perfringen is every liter of anolyte 10
5～10
7Individual somatic cells, the fuel that can be used as electron donor in the anolyte are citric acid, glycerol, glucose, sucrose or starch etc.
As the preferred version of present embodiment, the preparation of described anolyte may further comprise the steps:
(1) according to following proportioning preparation A liquid:
880 milliliters of deionized waters;
Sodium hydrogen phosphate 13.20 grams;
Sodium dihydrogen phosphate 2.83 grams;
Ammonium chloride 0.28 gram;
Potassium chloride 0.11 gram;
Sodium chloride 6.43 grams;
10 milliliters of trace element solutions;
10 milliliters of vitamin solutions;
Wherein, described trace element solution is prepared according to following proportioning:
1000 milliliters of deionized waters;
Sodium ethylene diamine tetracetate 1.5 grams;
Epsom salt 3.0 grams;
Manganese sulfate monohydrate 0.5 gram;
Sodium chloride 1.0 grams;
Green-vitriol 0.1 gram;
CoCL2 0.1 gram;
Calcium chloride 0.1 gram;
White vitriol 0.1 gram;
Cupric sulfate pentahydrate 0.1 gram;
Potassium aluminum sulfate dodecahydrate 0.01 gram;
Boric acid 0.01 gram;
Sodium Molybdate Dihydrate 0.01 gram;
Described vitamin solution is prepared according to following proportioning:
2.0 milligrams of biotins;
2.0 milligrams in folic acid;
The hydrochloric acid Cobastab
610.0 milligram;
5.0 milligrams of thiamine hydrochlorides;
5.0 milligrams in riboflavin;
5.0 milligrams in nicotinic acid;
5.0 milligrams of calcium pantothenates;
Cobastab
120.1 milligram;
5.0 milligrams of phosphorus aminobenzoic acids;
5.0 milligrams of lipoic acids;
(2) add fuel preparation B liquid in the deionized water;
For example in 100 ml deionized water, add 3 gram glucose, perhaps add 0.18 gram glucose, or 0.36 gram maltose, or 0.34 gram sucrose, or 3 gram starch, or the like, different B liquid prepared.
(3) A liquid, B liquid are sterilized respectively, the mixing of cooling back promptly gets anolyte.
The present invention provides simultaneously and has utilized the microbiological fuel cell of clostridium perfringen for anode catalyst, comprise anode, anode chamber, negative electrode and exchange membrane, connect yin, yang the two poles of the earth with lead and form external circuit, pack in the anode chamber clostridium perfringen somatic cells and the anolyte that contains fuel, sealing; Described anode adopts common carbon felt, and described negative electrode adopts the platinum plating carbon paper.Its electrogenesis principle is: organic substance acts as a fuel in the anaerobism anode chamber by the clostridium perfringen oxidation, produce electronics and proton, wherein, electronics is caught and passes to galvanic anode by clostridium perfringen, electronics arrives negative electrode by external circuit, thereby form the loop and produce electric current, and proton arrives negative electrode by exchange membrane, with the oxygen water generation reaction.
Compared with prior art, the present invention has following beneficial effect:
(1) utilize the anode catalyst of clostridium perfringen XM02 as microbiological fuel cell, this Pseudomonas is easy to cultivate in facultative anaerobe, can inactivation under the oxygen existence condition, can be by the regular culture matrix that adds, its electrogenesis activity of long term maintenance, operating cost is lower.
(2) the fuel utilization of clostridium perfringen XM02 spectrum is wider, can utilize multiple organic substance electrogenesis such as citric acid, glycerol, glucose, sucrose, starch, and oxidation operation is thorough, and the electronics rate of recovery is higher.
(3) the present invention has opened up new application for clostridium perfringen, for MFC provides a kind of new stronger electrogenesis microbe of applicability.
Description of drawings
Fig. 1 is the microbiological fuel cell structure principle chart of anode catalyst with the clostridium perfringen
The active figure of Fig. 2 clostridium perfringen electrogenesis
Fig. 3 clostridium perfringen utilizes glucose to be fuel electrogenesis situation
Fig. 4 clostridium perfringen utilizes maltose to be fuel electrogenesis situation
Fig. 5 clostridium perfringen utilizes sucrose to be fuel electrogenesis situation
Fig. 6 clostridium perfringen utilizes starch to be fuel electrogenesis situation
Embodiment
Further describe the present invention below in conjunction with the drawings and specific embodiments.
The active checking of embodiment 1 clostridium perfringen electrogenesis
(1) makes up microbiological fuel cell
Present embodiment has made up the microbiological fuel cell that utilizes the clostridium perfringen generating according to prior art and method, as shown in Figure 1, comprises anode chamber, cathode chamber, exchange membrane and external circuit four parts.With common carbon felt is anode electrode, as cathode electrode, connects yin, yang the two poles of the earth with lead with the platinum plating carbon paper.
(2) be seeded to the conventional LB fluid nutrient medium from the inclined-plane of preserving clostridium perfringen XM02, shaking table activation thalline is 16 hours under 30 ℃, 200 rev/mins conditions, reaching the exponential growth after date stops to cultivate, bacterium liquid was 4 ℃ of following 8000 rev/mins of high speed centrifugations 10 minutes, SPSS washing with 0.85% 2 times, again be suspended in 0.85% SPSS, as the microbe fuel cell inoculation thing;
(3) add the anolyte that contains fuel prepare in the anode chamber, inoculate clostridium perfringen then, make that thalline quantity reaches 5 * 10 in every liter of anolyte
6Individual, leave standstill cultivation under 30 ℃;
(4) anode, negative electrode are connected the on-line monitoring output voltage by 500 ohm of extrernal resistances.
Wherein, the compound method of anolyte (a) is as follows:
(1) preparation A liquid: add 13.197 gram sodium hydrogen phosphates, 2.831 gram sodium dihydrogen phosphates, 0.275 gram ammonium chloride, 0.11 gram potassium chloride, 6.428 gram sodium chloride, 10 milliliters of trace element solutions and 10 milliliters of vitamin solutions in 880 ml deionized water, be hybridly prepared into A liquid;
(2) preparation B liquid: add 3 gram glucose in 100 ml deionized water;
(3) A liquid, B liquid are sterilized according to laboratory, this area is conventional respectively, to be cooled to 30 ℃ of mixing.
According to the output voltage data, calculate the electronics rate of recovery and obtain the electrogenesis polarization curve, concrete outcome is seen shown in Figure 2, the result shows that maximum power density is 280mW/m
2, the electronics rate of recovery is 62.5%.
Embodiment 2 clostridium perfringen utilize the electrogenesis experiment of glucose for fuel
Experimental technique and step are with embodiment 1.
The preparation of anolyte (b):
(1) preparation A liquid, method is with embodiment 1;
(2) preparation B liquid: add 0.18 gram glucose in 100 ml deionized water;
(3) with A liquid, B liquid respectively according to the conventional sterilization in laboratory, this area, to be cooledly obtain anolyte (b) to mixed at room temperature.
Add 80 milliliters of anolytes (b) in the anode chamber, the inoculation clostridium perfringen makes that thalline quantity reaches 5 * 10 in every liter of anolyte
5Individual, leave standstill cultivation under 30 ℃; Anode, negative electrode connect by 500 ohm extrernal resistance, monitor its output voltage, and the result as shown in Figure 3.
Embodiment 3 clostridium perfringen utilize the experiment of maltose electrogenesis
Experimental technique and step be with embodiment 1, and the preparation of different is B liquid is to add 0.36 gram maltose in 100 ml deionized water; Output voltage changes as shown in Figure 4.
Embodiment 4 clostridium perfringen utilize the experiment of sucrose electrogenesis
Experimental technique and step be with embodiment 1, and the preparation of different is B liquid is to add 0.34 gram sucrose in 100 ml deionized water; Output voltage changes as shown in Figure 5.
Embodiment 5 clostridium perfringen utilize the experiment of starch electrogenesis
Experimental technique and step be with embodiment 1, and the preparation of different is B liquid is to add 3 gram starch in 100 ml deionized water, and output voltage changes as shown in Figure 6.
Claims (5)
1, the application of a kind of clostridium perfringen is characterized in that being applied to electricity generation by microorganism as the anode catalyst of microbiological fuel cell.
2, the described clostridium perfringen of a kind of claim 1 is applied to the electricity-generating method of electricity generation by microorganism, it is characterized in that may further comprise the steps:
(1) makes up microbiological fuel cell;
(2) preparation clostridium perfringen thalline is as the microbe fuel cell inoculation thing;
(3) preparation contains the anolyte of fuel;
(4) clostridium perfringen inoculum and the anolyte for preparing are added in the anode of microbial fuel cell chamber, leave standstill and carry out electrogenesis after the cultivation and detect.
3, be applied to the electricity-generating method of electricity generation by microorganism according to the described clostridium perfringen of claim 2, it is characterized in that the described preparation of step (2) clostridium perfringen somatic cells is to be seeded to the conventional LB fluid nutrient medium from the inclined-plane of preserving clostridium perfringen XM02, shaking table activation thalline 16h under 30 ℃, 200 rev/mins conditions, reaching the exponential growth after date stops to cultivate, bacterium liquid was 4 ℃ of following 8000 rev/mins of high speed centrifugations 10 minutes, with 0.85% SPSS washing 2 times, be suspended in 0.85% SPSS again and prepare inoculum.
4, be applied to the electricity-generating method of electricity generation by microorganism according to the described clostridium perfringen of claim 2, it is characterized in that the described fuel of described step (3) is citric acid, glycerol, glucose, sucrose or starch.
5, be applied to the electricity-generating method of electricity generation by microorganism according to the described clostridium perfringen of claim 2, it is characterized in that the described clostridium perfringen of step (3) is with every liter of anolyte 10
5～10
7The inoculum concentration of individual thalline adds in the anode of microbial fuel cell chamber.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810029222A CN100578851C (en) | 2008-07-04 | 2008-07-04 | Application of enterobacter aerogenes in microbe power generation and its power generation method |
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| CN200810029222A CN100578851C (en) | 2008-07-04 | 2008-07-04 | Application of enterobacter aerogenes in microbe power generation and its power generation method |
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| CN101320820A true CN101320820A (en) | 2008-12-10 |
| CN100578851C CN100578851C (en) | 2010-01-06 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102255096A (en) * | 2010-05-21 | 2011-11-23 | 中国科学院成都生物研究所 | Microbial fuel cell and preparation method thereof |
| CN109694837A (en) * | 2019-03-01 | 2019-04-30 | 中国科学院烟台海岸带研究所 | A kind of electricity production bacterial strain of degradable organic pollutant and its application |
-
2008
- 2008-07-04 CN CN200810029222A patent/CN100578851C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102255096A (en) * | 2010-05-21 | 2011-11-23 | 中国科学院成都生物研究所 | Microbial fuel cell and preparation method thereof |
| CN109694837A (en) * | 2019-03-01 | 2019-04-30 | 中国科学院烟台海岸带研究所 | A kind of electricity production bacterial strain of degradable organic pollutant and its application |
| CN109694837B (en) * | 2019-03-01 | 2022-05-06 | 中国科学院烟台海岸带研究所 | Electricity-generating strain for degrading organic pollutants and application thereof |
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| CN100578851C (en) | 2010-01-06 |
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