CN102255096A - Microbial fuel cell and preparation method thereof - Google Patents

Microbial fuel cell and preparation method thereof Download PDF

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Publication number
CN102255096A
CN102255096A CN2010101786723A CN201010178672A CN102255096A CN 102255096 A CN102255096 A CN 102255096A CN 2010101786723 A CN2010101786723 A CN 2010101786723A CN 201010178672 A CN201010178672 A CN 201010178672A CN 102255096 A CN102255096 A CN 102255096A
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fuel cell
preparation
pseudomonad
microbiological fuel
mbr
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李大平
张礼霞
张廷滔
何晓红
陶勇
王晓梅
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Chengdu Institute of Biology of CAS
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Chengdu Institute of Biology of CAS
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/30—Hydrogen technology
    • Y02E60/50—Fuel cells

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Abstract

The invention belongs to the technical field of bioenergy, and relates to a microbial fuel cell adopting pseudomonas alcaliphila sp. MBR as a catalyst, and a preparation method thereof. The microbial fuel cell adopts pseudomonas alcaliphila sp. MBR as an anode catalyst, wherein the preservation number of the pseudomonas alcaliphila sp. MBR is CGMCC NO.2318, and anode solution contains glucose, sodium citrate, cane sugar, fructose, xylose or soluble starch. The microbial fuel cell can work under aerobic, anoxic or amaerobic conditions and has the characteristics of non-harsh power production conditions, simple operation and low operation cost.

Description

A kind of microbiological fuel cell and preparation method thereof
Technical field
The invention belongs to the bioenergy technical field, being specifically related to a kind of is the microbiological fuel cell and preparation method thereof of catalyst with the pseudomonad.
Background technology
Along with the high speed development of society, and the trend of the exhaustion of fossil fuel and global warming forces countries in the world to pay much attention to the exploitation of renewable new forms of energy.(Microbial fuel cell is to utilize the electrogenesis microbe chemical energy in the organic substance to be converted into the device of electric energy as anode catalyst MFC) to microbiological fuel cell.
Organic waste with increase day by day in the problem of environmental pollution is a primary pollution source.
MFC can be implemented in the electric energy that produces cleaning when removing debirs.
Electrogenesis microbe as the MFC anode catalyst is a core.At present, the electrogenesis microbe of having found has strain more than 20, mainly concentrates on western Waksmania (Shewanella) and ground Bacillus (Geobacter).These bacterial strains only just have the electrogenesis activity under the strictly anaerobic condition, the existence of oxygen can be fought for electronics and be made its forfeiture electricity generation ability, so they are applied to MFC as catalyst and all are confined to anaerobic condition.And anaerobic condition can reduce microbe undoubtedly to organic clearance, also is unfavorable for practical application.Patent (publication number: reported CN 101320820A) that a facultative anaerobic bacterium is used for the MFC electrogenesis, do not reported the electrogenesis situation of this bacterium under aerobic condition people such as Zhou Shungui.
Pseudomonad is the facultative aerobe that occurring in nature extensively exists, existing many patent disclosures its at heavy metal biological reduction (publication number: CN101531970), biological desulphurization (CN1594549), biological denitrificaion (publication number: the purposes of aspect such as CN1460712), but the unexposed relevant purposes of pseudomonad aspect bioelectrogenesis, and, do not find that as yet pseudomonad all has electroactive report under aerobic, anoxic, the multiple condition of anaerobism through document and patent retrieval.
Summary of the invention
The purpose of this invention is to provide a kind of with a pseudomonas ( Pseudomonas alcaliphilaSp. be the microbiological fuel cell of catalyst MBR).
The present invention is achieved by the following technical programs:
Pseudomonad provided by the invention ( Pseudomonas alcaliphilaSp.) MBR is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms preservation center (being called for short CGMCC) on January 2nd, 2008, and preserving number is CGMCC No.2318.
Microbiological fuel cell of the present invention with pseudomonad ( Pseudomonas alcaliphilaSp.) MBR is as anode catalyst.
The preparation method of microbiological fuel cell of the present invention may further comprise the steps:
(1) makes up cell apparatus;
(2) preparation pseudomonad thalline is as the anode catalyst of microorganism battery
(3) configuration anolyte and catholyte;
(4) select operational mode, carry out electrogenesis and detect.
According to the described preparation of step (2) pseudomonad thalline is to be seeded to the Giltay culture medium of improvement from the inclined-plane of preserving pseudomonad, and shaking table activation thalline is 24 hours under 30 ℃, 130 rev/mins conditions, reaches the exponential growth after date and stops to cultivate.Bacterium liquid is made bacteria suspension 4 ℃, 8000 rev/mins high speed centrifugations 20 minutes with carbonate buffer solution.
The Giltay medium component of described improvement is: KNO 31.0g, KH 2PO 42.0g, MgSO 47H 20 1.0g, CaCl 22H 2O 0.2 g, FeCl 36H 2O 0.05g, natrium citricum 5.13g, distilled water 1L.
The described anolyte of step (3) is: NH 4Cl 0.53g, KH 2PO 42.0g, MgSO 47H 20 0.5g, FeCl 36H 2O 0.005g, CaCO 30.01 g, Na 2CO 33.18 g, NaHCO 35.88 g, glucose or/and natrium citricum or/and sucrose or/and fructose or/and wood sugar or/and soluble starch 2.0 g, distilled water 1L.
Catholyte is: Na 2HPO 421.84g, NaH 2PO 46.10 g, distilled water 1 L.
The described operational mode of step (4) is that aerobic condition (is that dissolved oxygen is at 0.5 mg L -1More than), anoxia condition (is that dissolved oxygen is at 0.2 ~ 0.5 mg L -1), anaerobic condition (is that dissolved oxygen is at 0.2 mg L -1Below).
Compared with prior art, the present invention has following advantage:
(1) utilize the anode catalyst of pseudomonad MBR as microbiological fuel cell, this Pseudomonas is easy to cultivate in amphimicrobe;
(2) the fuel utilization of pseudomonad MBR spectrum is wider, can utilize multiple organic substance electrogenesis such as glucose, citrate, fructose, wood sugar, sucrose, soluble starch, and oxidation operation is thorough, COD clearance height;
(3) the present invention's proposition utilizes and produces electric energy when the MFC of pseudomonad MBR inoculation all can reach the removal organic contamination under aerobic, anoxic, three kinds of conditions of anaerobism, and its electrogenesis condition is not harsh, and easy operating has reduced cost.
Description of drawings
Fig. 1 is for being the microbial fuel cell unit schematic diagram of anode catalyst with pseudomonad MBR, and wherein 1 is the anode chamber, and 2 is cathode chamber, and 3 is anode electrode, and 4 is cathode electrode, and 5 is barrier film;
Fig. 2 is the electrogenesis situation of fuel under aerobic condition for pseudomonad MBR utilizes glucose;
Fig. 3 is the electrogenesis situation of fuel under anoxia condition for pseudomonad MBR utilizes glucose;
Fig. 4 is fuel electrogenesis situation under anaerobic for pseudomonad MBR utilizes glucose;
Fig. 5 is the electrogenesis situation of fuel under anoxia condition for pseudomonad MBR utilizes fructose;
Fig. 6 is the electrogenesis situation of fuel under anoxia condition for pseudomonad MBR utilizes wood sugar;
Fig. 7 is the electrogenesis situation of fuel under anoxia condition for pseudomonad MBR utilizes natrium citricum;
Fig. 8 is the electrogenesis situation of fuel under anoxia condition for pseudomonad MBR utilizes starch.
Embodiment
Further describe the present invention below in conjunction with the drawings and specific embodiments.
Embodiment 1: pseudomonad MBR utilizes glucose to be the electrogenesis situation of fuel under aerobic condition.
(1) make up microbial fuel cell unit: present embodiment has made up the microbial fuel cell unit that utilizes pseudomonad MBR generating according to prior art and method, as shown in Figure 1, comprise anode chamber, cathode chamber, barrier film and external circuit four parts, 1 is the anode chamber among the figure, 2 is cathode chamber, 3 is anode electrode, and 4 is cathode electrode, and 5 is barrier film.The volume of chamber, yin, yang the two poles of the earth is 245ml, and yin, yang the two poles of the earth are 6 cm * 5 cm carbon felts, are barrier film with the cation-exchange membrane.
(2) preparation anolyte.A component: NH 4Cl 0.53 g, KH 2PO 42.0g, MgSO 47H 20 0.5 g, FeCl 36H 2O 0.005 g, CaCO 30.01 g, Na 2CO 33.18 g, NaHCO 35.88 g, distilled water 800 mL; The B component: glucose 2.0 g, distilled water 200 mL, A, B two components are sterilized respectively, and the mixing of cooling back promptly gets anolyte (pH 9.5).Preparation catholyte (pH 7.0): Na 2HPO 421.84g, NaH 2PO 46.10 g, distilled water 1 L.
(3) be seeded to the Giltay culture medium of improvement from the inclined-plane of preserving pseudomonad, shaking table activation thalline is 24 hours under 30 ℃, 130 rev/mins conditions, reaches the exponential growth after date and stops to cultivate.Bacterium liquid is made bacteria suspension 4 ℃, 8000 rev/mins high speed centrifugations 20 minutes with carbonate buffer solution.
(4) start microbiological fuel cell MFC1, respectively the above-mentioned catholyte of the above-mentioned anolyte of 230mL and 200mL is injected the chamber, negative and positive the two poles of the earth of battery, get above-mentioned pseudomonad suspension 2mL(again and contain 3.75mg albumen) inject the anode chamber of battery, and continue the air that exposes to the sun in the anode chamber, guarantee that dissolved oxygen is at 0.5 mg L -1More than, the cathode chamber air that exposes to the sun is an electron acceptor with the dissolved oxygen.Negative and positive the two poles of the earth connect with lead respectively, external 1 kW fixed resistance.
The output voltage of on-line monitoring battery when voltage drops to below the 20mV, is changed the anolyte of battery, guarantee to change behind the liquid service conditions with the first time entry condition identical, the electrogenesis curve in two cycles of battery operation is as shown in Figure 2.Before measuring anolyte respectively and injecting with on average the phase continues 5 days weekly through the COD(of battery operation after one-period), calculating COD, to remove speed be 294 mg L -1d -1, total clearance is 68%.
Embodiment 2: pseudomonad MBR utilizes glucose to be the electrogenesis situation of fuel under anoxia condition
Experimental technique and step are basic identical with the operation of MFC1 among the embodiment 1.The gap, anode chamber of different is microbiological fuel cell MFC2 among this embodiment air that exposes to the sun makes dissolved oxygen maintain 0.2 ~ 0.5 mg L -1Its electrogenesis curve as shown in Figure 3, on average about 10 days of phase weekly, it is 179 mg L that the organic COD of anode removes speed -1d -1, total clearance is 81%.
Embodiment 3: pseudomonad MBR utilizes glucose to be fuel electrogenesis situation under anaerobic
Experimental technique and step are basic identical with the operation of MFC1 among the embodiment 1.Different is when microbiological fuel cell MFC3 starts among this embodiment, and the logical nitrogen in anode chamber 20 minutes guarantees anaerobic condition.Its electrogenesis curve as shown in Figure 4, on average about 8 days of phase weekly, it is 272 mg L that the organic COD of anode removes speed -1d -1, total clearance is 83%.
Embodiment 4: pseudomonad MBR utilizes the electrogenesis situation of fructose under anoxia condition
The experimental technique of MFC4 and step are basic identical with the operation of MFC2 among the embodiment 2 among this embodiment.Different is to utilize fructose as carbon source, gained electrogenesis curve as shown in Figure 5, on average about 9 days of phase weekly, it is 232 mg L that the organic COD of anode removes speed -1d -1, total clearance is 89%.
Embodiment 5: pseudomonad MBR utilizes wood sugar to be the electrogenesis situation of fuel under anoxia condition
The experimental technique of MFC5 and step are basic identical with the operation of MFC2 among the embodiment 2 among this embodiment.Different is to utilize wood sugar as carbon source, gained electrogenesis curve as shown in Figure 6, on average about 8 days of phase weekly, it is 219 mg L that the organic COD of anode removes speed -1d -1, total clearance is 83%.
Embodiment 6: pseudomonad MBR utilizes natrium citricum to be the electrogenesis situation of fuel under anoxia condition
The experimental technique of MFC6 and step are basic identical with the operation of MFC2 among the embodiment 2 among this embodiment.Different is to utilize natrium citricum as carbon source, gained electrogenesis curve as shown in Figure 7, on average about 8 days of phase weekly, the organic COD clearance of anode is 512.5 mg L -1d -1, always removing efficient is 82%.
Embodiment 7: pseudomonad MBR utilizes starch to be the electrogenesis situation of fuel under anoxia condition
The experimental technique of MFC7 and step are basic identical with the operation of MFC2 among the embodiment 2 among this embodiment.Different is to utilize starch as carbon source gained electrogenesis curve as shown in Figure 8, and on average about 7 days of phase weekly, the organic COD clearance of anode is 271mg L -1d -1, always removing efficient is 92%.

Claims (8)

1. a microbiological fuel cell is characterized in that: with pseudomonad Pseudomonas alcaliphilaSp. MBR CGMCC No.2318 is an anode catalyst.
2. prepare the method for the described microbiological fuel cell of claim 1, may further comprise the steps:
(1) makes up cell apparatus;
(2) preparation pseudomonad thalline is as the anode catalyst of microorganism battery;
(3) configuration anolyte and catholyte;
(4) select operational mode, carry out electrogenesis detection and anolyte COD test.
3. the method for preparing microbiological fuel cell according to claim 2, it is characterized in that: described preparation pseudomonad thalline is to be seeded to the Giltay culture medium of improvement from the inclined-plane of preserving pseudomonad, shaking table activation thalline is 24 hours under 30 ℃, 130 rev/mins conditions, reaches the exponential growth after date and stops to cultivate; Bacterium liquid is made bacteria suspension 4 ℃, 8000 rev/mins high speed centrifugations 20 minutes with carbonate buffer solution.
4. the method for preparing microbiological fuel cell according to claim 3 is characterized in that: the composition of the Giltay culture medium of described improvement is: KNO 31.0g, KH 2PO 42.0g, MgSO 47H 20 1.0g, CaCl 22H 2O 0.2 g, FeCl 36H 2O 0.05g, natrium citricum 5.13g, distilled water 1L.
5. the preparation method of the described microbiological fuel cell of claim 2 is characterized in that: anolyte contain glucose or/and natrium citricum or/and sucrose or/and fructose or/and wood sugar or/and soluble starch.
6. according to the preparation method of claim 2 or 5 described microbiological fuel cells, it is characterized in that: described anolyte is: NH 4Cl 0.53g, KH 2PO 42.0g, MgSO 47H 20 0.5g, FeCl 36H 2O 0.005g, CaCO 30.01 g, Na 2CO 33.18 g, NaHCO 35.88 g, glucose or/and natrium citricum or/and sucrose or/and fructose or/and wood sugar or/and soluble starch 2.0 g, distilled water 1L; Catholyte is: Na 2HPO 421.84g, NaH 2PO 46.10 g, distilled water 1 L.
7. the preparation method of microbiological fuel cell according to claim 2, it is characterized in that: described operational mode is aerobic condition, anoxia condition, anaerobic condition.
8. the preparation method of microbiological fuel cell according to claim 7, it is characterized in that: described aerobic condition is that dissolved oxygen is at 0.5 mg L -1More than, anoxia condition is that dissolved oxygen is at 0.2 ~ 0.5 mg L -1, anaerobic condition is that dissolved oxygen is at 0.2 mg L -1Below.
CN2010101786723A 2010-05-21 2010-05-21 Microbial fuel cell and preparation method thereof Pending CN102255096A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103215301A (en) * 2013-01-29 2013-07-24 南京工业大学 Electricity-producing genetically engineered bacteria used in microbial fuel cell, and construction method and application thereof
CN103811791A (en) * 2014-01-29 2014-05-21 中国科学院成都生物研究所 Bioelectrochemistry device and bioelectrochemistry method for extracting reducing energy from waste and wastewater
EP2994537A4 (en) * 2013-03-15 2017-07-05 Microbial Robotics, LLC Compositions, systems and methods for protecting genetically modified organisms from unauthorized use or release into the environment
CN109755598A (en) * 2017-11-01 2019-05-14 西北农林科技大学 Charcoal mediates solid biologic membrane micro fuel cell promotor and preparation method
CN113732052A (en) * 2021-09-27 2021-12-03 中科云恒(成都)环境科技有限公司 Pseudomonas soil remediation agent and application thereof in remediation of heavy metal contaminated soil
CN115477381A (en) * 2022-08-16 2022-12-16 农业部沼气科学研究所 Preparation of oxygen-resistant multifunctional biological anode and sewage treatment method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101320820A (en) * 2008-07-04 2008-12-10 广东省生态环境与土壤研究所 Application of enterobacter aerogenes in microbe power generation and its power generation method
CN101485029A (en) * 2006-05-02 2009-07-15 宾夕法尼亚州研究基金会 Materials and configurations for scalable microbial fuel cells
CN101531970A (en) * 2008-03-12 2009-09-16 中国科学院成都生物研究所 Pseudomonas and application thereof in biological reduction and biological adsorption

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101485029A (en) * 2006-05-02 2009-07-15 宾夕法尼亚州研究基金会 Materials and configurations for scalable microbial fuel cells
CN101531970A (en) * 2008-03-12 2009-09-16 中国科学院成都生物研究所 Pseudomonas and application thereof in biological reduction and biological adsorption
CN101320820A (en) * 2008-07-04 2008-12-10 广东省生态环境与土壤研究所 Application of enterobacter aerogenes in microbe power generation and its power generation method

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103215301A (en) * 2013-01-29 2013-07-24 南京工业大学 Electricity-producing genetically engineered bacteria used in microbial fuel cell, and construction method and application thereof
CN103215301B (en) * 2013-01-29 2014-07-09 南京工业大学 Electricity-producing genetically engineered bacteria used in microbial fuel cell, and construction method and application thereof
EP2994537A4 (en) * 2013-03-15 2017-07-05 Microbial Robotics, LLC Compositions, systems and methods for protecting genetically modified organisms from unauthorized use or release into the environment
CN103811791A (en) * 2014-01-29 2014-05-21 中国科学院成都生物研究所 Bioelectrochemistry device and bioelectrochemistry method for extracting reducing energy from waste and wastewater
CN103811791B (en) * 2014-01-29 2015-12-09 中国科学院成都生物研究所 A kind of Bioelectrochemical device and method extracting also proper energy from discarded object and waste water
CN109755598A (en) * 2017-11-01 2019-05-14 西北农林科技大学 Charcoal mediates solid biologic membrane micro fuel cell promotor and preparation method
CN113732052A (en) * 2021-09-27 2021-12-03 中科云恒(成都)环境科技有限公司 Pseudomonas soil remediation agent and application thereof in remediation of heavy metal contaminated soil
CN115477381A (en) * 2022-08-16 2022-12-16 农业部沼气科学研究所 Preparation of oxygen-resistant multifunctional biological anode and sewage treatment method thereof

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Application publication date: 20111123