CN101313931A - Application of Heichou extract in preparing antineoplastic medicament - Google Patents
Application of Heichou extract in preparing antineoplastic medicament Download PDFInfo
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- CN101313931A CN101313931A CNA2008101156651A CN200810115665A CN101313931A CN 101313931 A CN101313931 A CN 101313931A CN A2008101156651 A CNA2008101156651 A CN A2008101156651A CN 200810115665 A CN200810115665 A CN 200810115665A CN 101313931 A CN101313931 A CN 101313931A
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- heichou
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- semen pharbitidis
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Abstract
The invention discloses a novel application for a pharbitis seed extract. The novel application is an application of the pharbitis seed extract in preparing an antitumor drug. The extract is prepared by the following method that: the pharbitis seed extract is extracted from the pharbitis seed by taking water and/or an organic solvent as an extracting agent. The pharbitis seed extract provided by the invention is greatly meaningful for preparing antitumor drugs, in particular drugs of resisting liver cancer, lung cancer and colon cancer.
Description
Technical field
The present invention relates to the application of a kind of Heichou extract in the preparation antitumor drug.
Background technology
Hepatocarcinoma is one of China's common malignancy, is that China occupies second cancer " killer ", is common in middle-aged male.Fast because of its grade of malignancy height, disease progression, patient does not generally have any discomfort in early days, but in case symptom occurs and go to a doctor, has just belonged to middle and advanced stage.So the treatment difficulty of hepatocarcinoma is big, life span only was 6 months after general patient fell ill, person " king in the cancer ".According to the survey showed that, the New Development cancer of male's front three is respectively pulmonary carcinoma, colon cancer and nasopharyngeal carcinoma at present, and the women then is breast carcinoma, colon cancer and pulmonary carcinoma, has proved absolutely the hazardness in New Development cancer group of pulmonary carcinoma and colon cancer.
From plant, seek the cancer therapy drug of high-efficiency low-toxicity, become the emphasis of domestic and international cancer therapy drug research.Effectively the anticancer compound preparation of anticancer compound and Chinese herbal medicine effective site all has important clinic value.
Semen Pharbitidis is the lead a cow black seed of (Pharbitis nil) of convolvulaceous plant, some that remove northwest and northeast in China outside the province, all there is distribution in most of area.According to " Chinese Plants will " record, the seed of leading a cow is a conventional Chinese medicine, name CHOUNIU (Yunnan), Semen Pharbitidis, Semen Pharbitidis, Semen Pharbitidis (black, the sub-mixing of white race), and use Semen Pharbitidis, the less usefulness of Semen Pharbitidis are used as medicine more.Semen Pharbitidis has the removing water retention by purgation diuresis, by expectorant, and parasiticidal effect.The application of Semen Pharbitidis in the preparation antitumor drug do not see that report is arranged.
Summary of the invention
A kind of new purposes that the purpose of this invention is to provide Heichou extract.
The purposes of Heichou extract provided by the present invention is the application of Heichou extract in the preparation antitumor drug; Described Heichou extract is prepared as follows: be water and/or organic solvent as extractant, from Semen Pharbitidis, extract and obtain Heichou extract.
Wherein, described organic solvent specifically can be methanol, ethanol, petroleum ether, chloroform, dichloromethane, ethyl acetate, n-butyl alcohol, benzene or toluene.
It is 95% ethanol water that described extractant specifically can be volumn concentration.
Described extraction can be reflux, extract.
The temperature of described extraction can be 60-100 ℃.
In the described extraction, extract once at least, each time of extracting is 1-4 hour.
Described tumor can be hepatocarcinoma, colon cancer or pulmonary carcinoma.
According to prepared the preventing and/or treating tumour medicine and can import body such as muscle, Intradermal, subcutaneous, vein, mucosal tissue of as above method by the method for injection, injection, collunarium, eye drip, infiltration, absorption, physics or chemistry mediation; Or mixed by other materials or wrap up the back and import body.
When needing, in said medicine, can also add one or more pharmaceutically acceptable carriers.Described carrier comprises diluent, excipient, filler, binding agent, wetting agent, disintegrating agent, absorption enhancer, surfactant, absorption carrier, lubricant of pharmaceutical field routine etc.
As above preparation prevents and/or treats tumour medicine and can make various ways such as injection, tablet, powder, granule, capsule, oral liquid, unguentum, cream.The medicine of above-mentioned various dosage forms all can be according to the conventional method preparation of pharmaceutical field.
The above-mentioned consumption that prevents and/or treats tumour medicine is generally 200-600mg Heichou extract/kg body weight/day, and be 10 to 15 days the course of treatment.
The method for preparing Heichou extract of the present invention is simple, with low cost.Experiment showed, that Heichou extract of the present invention can effectively suppress the growth of human tumor cells, as people's hepatocarcinoma, colon cancer or lung carcinoma cell.Therefore Heichou extract provided by the present invention is to the preparation antitumor drug, and especially anti-hepatocarcinoma, pulmonary carcinoma, colon cancer medicine have great importance.
The specific embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
The preparation of embodiment 1, Heichou extract
Get Semen Pharbitidis (Beijing TongrenTang Co., Ltd) 200g, (Semen Pharbitidis: ratio of weight and number 95% ethanol) and 95% ethanol (ethanol: water=95: 5, volume ratio) mixed, reflux, extract, 3h under 60 ℃ of conditions with 1: 3 with it, sucking filtration is collected reflux extracting liquid 1 and filtering residue 1 for the first time; All filtering residues 1 that obtain are mixed with 95% ethanol, and 95% alcoholic acid amount is with for the first time identical, and reflux, extract, 3h under 60 ℃ of conditions collects reflux extracting liquid 2 and filtering residue 2 for the second time, and extracting solution 2 and extracting solution 1 are merged; All filtering residues 2 that obtain are mixed with 95% ethanol, and 95% alcoholic acid amount is identical with the first time, 60 ℃ of reflux, extract, 3h, and sucking filtration is collected reflux extracting liquid 3 for the third time; Extracting solution 1, extracting solution 2 and extracting solution 3 are merged, the extracting solution of gained is carried out vacuum decompression concentrates, vacuum drying (removing the second alcohol and water) to constant weight, obtains the 4.1g Heichou extract.
Embodiment 2, the application of Heichou extract in suppressing growth of tumour cell
The trypsinization of tumor cell (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung carcinoma cell A-549) with logarithmic growth with 0.25%, then with the DMEM complete medium suspension cell that contains 10% (volumn concentration) hyclone, make cell suspension, and respectively each tumor cell is diluted to 1.0 * 10
5Individual/mL, again it is seeded in 96 well culture plates, every hole 200uL, each tumor cell inoculate 3 holes respectively, in 37 ℃, 5%CO
2Incubator in cultivate 24h; Each hole culture fluid then inclines.
The Heichou extract that embodiment 1 is made is dissolved among the DMSO, dilutes with the DMEM complete medium then, obtains Heichou extract solution, and making the final concentration of Heichou extract is 50ug/mL, and the concentration that makes DMSO is 10% (volumn concentration).
Establishing experimental group, matched group and blank group simultaneously experimentizes:
Experimental group: Heichou extract solution is added respectively successively in the culture hole of above-mentioned tumor cell HepG-2, BEL-7402, HCT-8, A-549, inoculate 3 holes separately, every hole adds 200ul;
Matched group: add the DMEM complete medium that contains 10% (volumn concentration) DMSO in the culture hole of above-mentioned each tumor cell, inoculate 3 holes separately, every hole adds 200ul;
Blank group: the DMEM complete medium that in the empty culture hole that does not contain tumor cell, adds the 200ul that contains 10% (volumn concentration) DMSO;
The culture plate of above each group is placed 37 ℃, 5%CO simultaneously
2Incubator in cultivated 2 days.
Add 20ul freshly prepared 5mg/ml tetramethyl azo azoles salt (MTT) solution to every hole after 2 days, and at 37 ℃, 5%CO
2Condition under continue to cultivate 4h; Carefully abandon supernatant, every hole adds 200uL DMSO, and room temperature is placed 15-20min, and every 5min vibration once; On microplate reader, measure the optical density value (OD value) of each hole, by formula calculate the growth of tumour cell suppression ratio at wavelength 490nm place.Suppression ratio (%)=1-(OD
Experiment-OD
Blank)/(OD
Contrast-OD
Blank) * 100%.
Three repetitions are established in experiment, and the result takes the mean.Heichou extract is as shown in table 1 to the inhibiting result of the different tumor cell HepG-2 of people, BEL-7402, HCT-8, A-549.
Table 1, Heichou extract are to the inhibitory action of the different tumor cells of people
| Tumor cell | Extract concentrations (ug/mL) | Suppression ratio (%) |
| HepG-2 (hepatocarcinoma) | 50 | -31.4 |
| BEL-7402 (hepatocarcinoma) | 50 | 48.18 |
| HCT-8 (colon cancer) | 50 | 36.66 |
| A-549 (pulmonary carcinoma) | 50 | 72.78 |
The inhibitory action experimental result to kinds of tumor cells of Heichou extract shows (table 1), Heichou extract when concentration is 50ug/mL to human liver cancer cell HepG-2 unrestraint effect, but BEL-7402 has better inhibited activity to hepatoma carcinoma cell, inhibitory action to lung carcinoma cell A-549 is the strongest, can reach 72.78%.This result shows that there is inhibitory action in various degree in Heichou extract to hepatocarcinoma, colon cancer, pulmonary carcinoma tumor cell, reaches as high as more than 72.78%.Therefore, Heichou extract can be used to prepare anti-hepatocarcinoma, colon cancer, lung cancer drugs.
Claims (10)
1, the application of Heichou extract in the preparation antitumor drug; Described Heichou extract is prepared as follows: be water and/or organic solvent as extractant, from Semen Pharbitidis, extract and obtain Heichou extract.
2, application according to claim 1 is characterized in that: described organic solvent is methanol, ethanol, petroleum ether, chloroform, dichloromethane, ethyl acetate, n-butyl alcohol, benzene or toluene.
3, application according to claim 1 is characterized in that: described extractant is that volumn concentration is 95% ethanol water.
4, according to arbitrary described application among the claim 1-3, it is characterized in that: the described reflux, extract, that is extracted as.
5, application according to claim 4 is characterized in that: the temperature of described extraction is 60-100 ℃.
6, according to claim 4 or 5 described application, it is characterized in that: in the described extraction, extract once at least, each time of extracting is 1-4 hour.
7, according to arbitrary described application in the claim 1 to 6, it is characterized in that: described tumor is hepatocarcinoma, colon cancer or pulmonary carcinoma.
8, a kind of Heichou extract, described Heichou extract be with volumn concentration be 95% ethanol water as extractant, from Semen Pharbitidis, extract and to obtain.
9, Heichou extract according to claim 8 is characterized in that: the described reflux, extract, that is extracted as; The temperature of described extraction is 60-100 ℃.
10, Heichou extract according to claim 9 is characterized in that: in the described extraction, extract once at least, each time of extracting is 1-4 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810115665 CN101313931B (en) | 2008-06-26 | 2008-06-26 | Application of Heichou extract in preparing antineoplastic medicament |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810115665 CN101313931B (en) | 2008-06-26 | 2008-06-26 | Application of Heichou extract in preparing antineoplastic medicament |
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| Publication Number | Publication Date |
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| CN101313931A true CN101313931A (en) | 2008-12-03 |
| CN101313931B CN101313931B (en) | 2012-12-26 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558220A (en) * | 2013-10-25 | 2015-04-29 | 匡海学 | Method for preparing galactomannan from morning glory seed |
| CN109010410A (en) * | 2018-08-03 | 2018-12-18 | 广西壮族自治区药用植物园 | For treating the drug and preparation method and application of colon cancer |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1308661C (en) * | 1987-06-26 | 1992-10-13 | Masayasu Kurono | AGENT FOR INHIBITING BINDING OF 5.alpha.-DIHYDRO-TESTOSTERONE WITH ANDROGEN RECEPTOR AS WELL AS PROCESS FOR OBTAINING SAME |
| CN1128662A (en) * | 1995-02-11 | 1996-08-14 | 晚霞工程组织委员会 | Oral liquid for curing cancer and prepn. method thereof |
| US6790464B2 (en) * | 2003-01-16 | 2004-09-14 | Healthaid Enterprise Pte. Ltd. | Herbal compositions for prostate conditions |
| CN100509041C (en) * | 2006-12-28 | 2009-07-08 | 杨长志 | Stomach-clearing pill |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104558220A (en) * | 2013-10-25 | 2015-04-29 | 匡海学 | Method for preparing galactomannan from morning glory seed |
| CN109010410A (en) * | 2018-08-03 | 2018-12-18 | 广西壮族自治区药用植物园 | For treating the drug and preparation method and application of colon cancer |
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| CN101313931B (en) | 2012-12-26 |
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