CN101347475B - Use of Alstonia mairei extract in preparing anti-tumor medicament - Google Patents
Use of Alstonia mairei extract in preparing anti-tumor medicament Download PDFInfo
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- CN101347475B CN101347475B CN2008101194009A CN200810119400A CN101347475B CN 101347475 B CN101347475 B CN 101347475B CN 2008101194009 A CN2008101194009 A CN 2008101194009A CN 200810119400 A CN200810119400 A CN 200810119400A CN 101347475 B CN101347475 B CN 101347475B
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- alstonia
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- mairei
- colon cancer
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Abstract
The present invention discloses a new usage of alstonia extract. The new usage is the application of alstonia extract in preparing anti-tumor drugs. The extract is prepared according to the following method: taking alstonia as the raw material and extracting by water and/or organic solvent. The alstonia extract provided by the present invention plays an important role in preparing anti-tumor drugs, especially the drugs for resisting liver cancer, lung cancer and colon cancer.
Description
Technical field
The present invention relates to the application of Alstonia mairei extract in the preparation antitumor drug.
Background technology
Hepatocarcinoma is one of China's common malignancy, is that China occupies second cancer " killer ", is common in middle-aged male.Fast because of its grade of malignancy height, disease progression, patient does not generally have any discomfort in early days, but in case symptom occurs and go to a doctor, has just belonged to middle and advanced stage.So the treatment difficulty of hepatocarcinoma is big, life span only was 6 months after general patient fell ill, person " king in the cancer ".According to the survey showed that, the New Development cancer of male's front three is respectively pulmonary carcinoma, colon cancer and nasopharyngeal carcinoma at present, and the women then is breast carcinoma, colon cancer and pulmonary carcinoma, has proved absolutely the hazardness in New Development cancer group of pulmonary carcinoma and colon cancer.
From plant, seek the cancer therapy drug of high-efficiency low-toxicity, become the emphasis of domestic and international cancer therapy drug research.Effectively the anticancer compound preparation of anticancer compound and Chinese herbal medicine effective site all has important clinic value.
Cornu Caprae seu Ovis cotton (Alstonia mairei L.C.) is called Folium Alstoniaer Mairei, Radix Zanthoxyli Spinifolii (Guizhou), Ji Shetoushu (Yunnan) again, for Apocynaceae Alstonia plant, originates in Yunnan, Guizhou, Sichuan, is born under the sparse woods of height above sea level 700-1500 rice mountain region on the rock.According to " Chinese Plants will " record, the root of Cornu Caprae seu Ovis cotton, leaf smash among the people that to be used to treat knife injury hemorrhage and control sore to pieces.The application of Cornu Caprae seu Ovis cotton in the preparation antitumor drug do not see that report is arranged.
Summary of the invention
A kind of new purposes that the purpose of this invention is to provide Alstonia mairei extract.
The purposes of Alstonia mairei extract provided by the present invention is the application of Alstonia mairei extract in the preparation antitumor drug; Described Alstonia mairei extract is prepared as follows: water and/or organic solvent extract from the Cornu Caprae seu Ovis cotton and obtain Alstonia mairei extract as extractant.
Wherein, described organic solvent specifically can be methanol, ethanol, petroleum ether, chloroform, dichloromethane, ethyl acetate, n-butyl alcohol, benzene or toluene.It is 95% ethanol water that described extractant specifically can be volumn concentration.
Described extraction can be reflux, extract.
In the described extraction, the temperature of extraction can be 60-100 ℃.Described extraction is extracted once at least, and each time of extracting is 1-4 hour.
As above arbitrary described in, described tumor specifically can be hepatocarcinoma, colon cancer or pulmonary carcinoma, is preferably hepatocarcinoma or colon cancer.。
According to prepared the preventing and/or treating tumour medicine and can import body such as muscle, Intradermal, subcutaneous, vein, mucosal tissue of as above method by the method for injection, injection, collunarium, eye drip, infiltration, absorption, physics or chemistry mediation; Or mixed by other materials or wrap up the back and import body.
When needing, in said medicine, can also add one or more pharmaceutically acceptable carriers.Described carrier comprises diluent, excipient, filler, binding agent, wetting agent, disintegrating agent, absorption enhancer, surfactant, absorption carrier, lubricant of pharmaceutical field routine etc.
As above preparation prevents and/or treats tumour medicine and can make various ways such as injection, tablet, powder, granule, capsule, oral liquid, unguentum, cream.The medicine of above-mentioned various dosage forms all can be according to the conventional method preparation of pharmaceutical field.
The above-mentioned consumption that prevents and/or treats tumour medicine is generally 200-800mg Alstonia mairei extract/kg body weight/day, and be 10 to 15 days the course of treatment.
The method for preparing Alstonia mairei extract of the present invention is simple, with low cost.Experiment showed, that Alstonia mairei extract of the present invention can effectively suppress the growth of human tumor cells, as people's hepatocarcinoma, colon cancer or lung carcinoma cell.Therefore Alstonia mairei extract provided by the present invention is to the preparation antitumor drug, and especially anti-hepatocarcinoma, pulmonary carcinoma, colon cancer medicine have great importance.
The specific embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
The preparation of embodiment 1, Alstonia mairei extract
Get Cornu Caprae seu Ovis cotton (Ningbo De Kang biological product company limited) 200g, (the Cornu Caprae seu Ovis cotton: ratio of weight and number 95% ethanol) and 95% ethanol (ethanol: water=95: 5, volume ratio) mixed, reflux, extract, 3h under 60 ℃ of conditions with 1: 3 with it, sucking filtration is collected reflux extracting liquid 1 and filtering residue 1 for the first time; All filtering residues 1 that obtain are mixed with 95% ethanol, and 95% alcoholic acid amount is extracted 3h with for the first time identical under 60 ℃ of conditions, collects reflux extracting liquid 2 and filtering residue 2 for the second time, and extracting solution 2 and extracting solution 1 are merged; All filtering residues 2 that obtain are mixed with 95% ethanol, and 95% alcoholic acid amount is identical with the first time, 60 ℃ of reflux, extract, 3h, and sucking filtration is collected reflux extracting liquid 3 for the third time; Extracting solution 1, extracting solution 2 and extracting solution 3 are merged, the extracting solution of gained is carried out vacuum decompression concentrates, vacuum drying (removing the second alcohol and water) to constant weight, obtains the 4.7g Alstonia mairei extract.
Embodiment 2, the application of Alstonia mairei extract in suppressing growth of tumour cell
The trypsinization of tumor cell (human liver cancer cell HepG-2, human liver cancer cell BEL-7402, human colon cancer cell HCT-8, human lung carcinoma cell A-549) with logarithmic growth with 0.25%, then with the DMEM complete medium suspension cell that contains 10% (volumn concentration) hyclone, make cell suspension, and respectively each tumor cell is diluted to 1.0 * 10
5Individual/mL, again it is seeded in 96 well culture plates, every hole 200uL, each tumor cell inoculate 6 holes respectively, in 37 ℃, 5%CO
2Incubator in cultivate 24h; Each hole culture fluid then inclines.
The Alstonia mairei extract that embodiment 1 is made is dissolved among the DMSO, dilute with the DMEM complete medium then, obtain the solution of Alstonia mairei extract, the final concentration of Alstonia mairei extract is 50ug/mL, wherein, the concentration of DMSO is 10% (volumn concentration).
Establish experimental group simultaneously, matched group experimentizes:
Experimental group: Alstonia mairei extract solution is added respectively successively in the culture hole of above-mentioned tumor cell HepG-2, BEL-7402, HCT-8, A-549, inoculate 3 holes separately, every hole adds 200ul;
Matched group: add the DMEM complete medium that contains 10% (volumn concentration) DMSO in the culture hole of above-mentioned each tumor cell, inoculate 3 holes separately, every hole adds 200ul;
The culture plate of above each group is placed 37 ℃, 5%CO simultaneously
2Incubator in cultivated 2 days.
Add 20ul freshly prepared 5mg/ml tetramethyl azo azoles salt (MTT) solution to every hole after 2 days, and at 37 ℃, 5%CO
2Condition under continue to cultivate 4h; Carefully abandon supernatant, every hole adds 200uL DMSO, and room temperature is placed 15-20min, and every 5min vibration once; On microplate reader, measure the optical density value (OD value) of each hole, by formula calculate the growth of tumour cell suppression ratio at wavelength 490nm place.Suppression ratio (%)=(OD
Contrast-OD
Experiment)/OD
Contrast* 100%.
Three repetitions are established in experiment, and the result takes the mean.Alstonia mairei extract is as shown in table 1 to the inhibiting result of the different tumor cell HepG-2 of people, BEL-7402, HCT-8, A-549.
Table 1, Alstonia mairei extract are to the inhibitory action of the different tumor cells of people
Alstonia mairei extract shows (seeing Table 1) to the inhibitory action experimental result of kinds of tumor cells, Alstonia mairei extract all has inhibitory action to a certain degree to human liver cancer cell BEL-7402, colon cancer cell HCT-8 when concentration is 50ug/mL, less to the inhibitory action of human liver cancer cell HepG-2 and human lung carcinoma cell A-549.Therefore, Alstonia mairei extract can be used to prepare the medicine of anti-hepatocarcinoma and colon cancer.
Claims (5)
1. the application of Alstonia mairei extract in the preparation antitumor drug; Described Alstonia mairei extract is prepared as follows: with volumn concentration be 95% ethanol water as extractant, from the Cornu Caprae seu Ovis cotton, extract and obtain Alstonia mairei extract;
Described tumor is hepatocarcinoma, colon cancer or pulmonary carcinoma.
2. application according to claim 1 is characterized in that: the described reflux, extract, that is extracted as.
3. application according to claim 2 is characterized in that: the temperature of described extraction is 60-100 ℃.
4. according to claim 2 or 3 described application, it is characterized in that: in the described extraction, extract once at least, each time of extracting is 1-4 hour.
5. application according to claim 1 is characterized in that: described tumor is hepatocarcinoma or colon cancer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008101194009A CN101347475B (en) | 2008-09-05 | 2008-09-05 | Use of Alstonia mairei extract in preparing anti-tumor medicament |
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| CN2008101194009A CN101347475B (en) | 2008-09-05 | 2008-09-05 | Use of Alstonia mairei extract in preparing anti-tumor medicament |
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| CN101347475A CN101347475A (en) | 2009-01-21 |
| CN101347475B true CN101347475B (en) | 2011-08-10 |
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| CN116969945B (en) * | 2023-09-15 | 2023-12-01 | 山东省中医药研究院 | Indole alkaloid compound and extraction method and application thereof |
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2008
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Non-Patent Citations (2)
| Title |
|---|
| 叶建华等.见血飞化学成份的研究I.《化学学报》.1989,(第47期),第1012-1016页. * |
| 李朝明,苏健,穆青,郑惠兰,吴曙光.羊角棉根的吲哚生物碱.《云南植物研究》.1998,第20卷(第2期),244-246. * |
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