CN101311727B - Fluid handling unit and fluid handling apparatus using same - Google Patents
Fluid handling unit and fluid handling apparatus using same Download PDFInfo
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- CN101311727B CN101311727B CN 200810108740 CN200810108740A CN101311727B CN 101311727 B CN101311727 B CN 101311727B CN 200810108740 CN200810108740 CN 200810108740 CN 200810108740 A CN200810108740 A CN 200810108740A CN 101311727 B CN101311727 B CN 101311727B
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Abstract
A fluid handling unit (16) has an outside large-diameter cylindrical portion (16a), an outside small-diameter cylindrical portion 16b and an inside cylindrical portion (16c), which are integral-molded so as to be integrated with each other. The inside cylindrical portion (16c) has a plurality of slits 16d, which are extend from the lower end of the inside cylindrical portion (16c) to the upper end thereof, for establishing a communication between an inside fluid housing chamber (30), which is formed in the inside cylindrical portion (16c), and an outside fluid housing chamber (28) which is formed between the inside cylindrical portion (16c) and the outside small-diameter cylindrical portion 16b. The plurality of slits 16d are designed to cause the most part of a liquid in the inside fluid housing chamber (30) to enter the outside fluid housing chamber (28) due to capillarity when the quantity of the liquid fed into the fluid handling unit 16 is not larger than a predetermined quantity, and to allow the liquid in the outside fluid housing chamber (28) to enter the inside fluid housing chamber when the quantity of the liquid fed into the fluid handling unit 16 exceeds the predetermined quantity.
Description
Technical field
The present invention relates generally to fluid processing unit and uses the fluid treating device of this unit.Specifically, the present invention relates to be used as analytical sample a sample analyzer part fluid processing unit and use the fluid treating device of this unit, described sample is such as the biological substance that represents functional materials.
Background technology
Just be used for detecting especially the classic method of biological substance such as protein, the known reaction that has the whole bag of tricks to cause Ag-Ab, it uses antibody to act on specific biological substance, in order to thus obtained reactant is implemented naked eyes identification or spectroscope measurement, thereby detects this biological substance.
As the method that quantitatively obtains reactant quantity by the antigen-antibody reaction of biological substance (such as protein), adopted some method such as ELISA (the immunosorbent chemical examination that enzyme connects) widely.In these methods, used a kind of sample analyzer that is referred to as microplate, wherein, arranging many small pit portion, they are referred to as little well (hereinafter will be called " well ") usually.The wall surface of Zhu Jing is coated the antibody with particular organisms material (target substance) effect, it is as a kind of capturing (or catching) material, utilize this trapping material to capture (or catching) target substance, detect target substance by measuring reactant, the acquisition of reactant is to utilize fluorescence, luminous or suchlike reactant between target substance and antibody antigen-antibody reaction to take place.
In the typical method of the use microplate such such as ELISA, fill such as the such liquid of sample that contains target substance or antibody response thing in the well, its as reaction solution to cause reaction.This reaction just takes place when the various compositions in the filling liquid arrive shaft bottom and inwall by the molecular diffusion motion in well.Reason for this reason, if microplate is erect, then Li Lun reaction time is depended on the diffusion time of various compositions in the interior filling liquid of well.Because molecular motion in the liquid, simultaneously with molecular collision on every side, so rate of propagation is very slow.Be about 70,000 protein if target substance is molecular weight, then (room temperature) rate of propagation is about 0.5 to 1 * 10 in dilute aqueous
-6Cm
2/ sec.Therefore, be filled in the liquid in the well, be difficult to react in the Measuring Time of reality away from the target substance of shaft bottom and inwall.In addition and since be effectively to make as surface, the shaft bottom of reactive moieties and wall surface equably contact reaction solution in order to improve reaction efficiency in the microplate, so, require a large amount of ratio of use to react the big amount of liquid of required amount of liquid.
Therefore, in the classic method such as the use microplate of ELISA, antigen-antibody reaction is only carried out at the wall surface of the well that scribbles trapping antibody.Therefore, liquid is erect, up to target substance, antibody and be fed to that contained nutrient culture media suspends in the liquid in the well, circulation and sink to arriving borehole wall surface till, so, exist the problem of reaction efficiency difference.In addition, in being subdivided into the microplate of a large amount of wells, the amount of liquid that is fed in each well is very limited, so the problem of existence is to measure sensitivity to reduce.
In order to improve the Measuring Time of measuring sensitivity and shortening ELISA etc., someone has proposed a kind of microplate that can increase the surface area of reaction surface (capturing the surface), it improves measurement sensitivity by form small irregular shape (for example, seeing the open No.9-159673 of Jap.P.) at each shaft bottom face as reaction surface.The somebody has proposed a kind of microplate that can increase the surface area of reaction surface, it improves reaction efficiency (for example, seeing the open No.2001-4628 of Jap.P.) in the trickle space by arrange small full particle (microballon) in the microflute of microplate as the solid phase of reaction.In addition, also proposed a kind of microplate that can increase the surface area of reaction surface and save sample size, it is by the depressed part that forms minor diameter in the core in each shaft bottom assign to achieve the above object (for example, seeing the open No.9-101302 of Jap.P.).
Yet in the microplate that proposes in the open No.9-159673 of Jap.P., the problem of existence is, although it can improve measurement sensitivity, it can not improve reaction efficiency.In addition, the microplate that proposes among the open No.2001-4628 of Jap.P. is not suitable for measures a large amount of samples, although it can improve reaction efficiency, because it is the microplate with micro groove structure, rather than is generally used for the microplate of ELISA and so on.In addition, in the microplate that proposes in the open No.9-101302 of Jap.P., improve reaction efficiency although it can improve the surface area of reaction surface to a certain extent and measure sensitivity, it can't sufficiently improve reaction efficiency and measure sensitivity.
Summary of the invention
Therefore, the objective of the invention is to eliminate above-mentioned problem and provide a kind of for the fluid processing unit in the fluid treating device and the fluid treating device that uses this unit, when this device was used as the device of measuring a large amount of samples, this device can improve reaction efficiency and measurement sensitivity and shorten the reaction time and Measuring Time with simple structure.
Another object of the present invention is to allow above-mentioned fluid processing unit or use the fluid treating device of this fluid processing unit can further improve analysis precision, and the reactant or the sample size that namely are used in analysis are considerably less, also can reach high precision.
In order to realize above-mentioned purpose with other, according to an aspect of the present invention, a kind of fluid processing unit comprises: vessel, and it has for the bottom and the sidepiece that wherein form the fluid containment part, and this vessel has opening in the top; The partition wall portions of extending from the bottom is used for the fluid containment of vessel partly is separated into first fluid container cavity and the second fluid containment chamber; And by partition wall portions and the communication passage that between first fluid container cavity and the second fluid containment chamber, set up to be communicated with, wherein, communication passage links to each other with the first and second fluid containment chambeies, when being not more than scheduled volume for the amount of liquid when be fed to the fluid containment part from the container body openings in, make the liquid in the first fluid container cavity can enter into the second fluid containment chamber because of capillary action, stop the liquid in the second fluid containment chamber to enter the first fluid container cavity simultaneously, and the amount of liquid when be fed to fluid containment part from the container body openings in then allows the liquid in the second fluid containment chamber to enter the first fluid container cavity during above scheduled volume.
In this fluid processing unit, the height of partition wall portions is preferably lower than the height of vessel lateral parts.Communication passage preferably includes one or more slits, and they pass through partition wall portions, and extends to its upper end from the bottom of partition wall portions.
In above-mentioned fluid processing unit, the first fluid container cavity is preferably surrounded by the second fluid containment chamber.In this case, vessel preferably has cylinder form roughly, and partition wall portions preferably has coaxial with vessel basically substantial cylindrical shape.Vessel preferably has the major diameter part of substantial cylindrical and the small diameter portion of substantial cylindrical, the small diameter portion of substantial cylindrical is arranged in the major diameter part below of substantial cylindrical, and partition wall portions preferably is arranged in the small diameter portion of substantial cylindrical.Communication passage preferably comprises a plurality of slits, and they are arranged with equidistant intervals along the circumferencial direction of partition wall portions.Partition wall portions preferably has towards interior downward-sloping upper surface.
In above-mentioned fluid processing unit, the bottom surface portions in the second fluid containment chamber is along with from the reducing of distance of first fluid container cavity and downward-sloping, and the height of the lowermost part of the second fluid containment chamber bottom surface portions is preferably substantially equal to the height of the lowermost part of first fluid container cavity bottom surface portions.Each slit at the width on that side of first fluid container cavity preferably greater than the width on second that side of fluid containment chamber.When the amount of liquid when be fed to the fluid containment part from the container body openings in was not more than scheduled volume, most of liquid preferably entered into the second fluid containment chamber in the first fluid container cavity.Fluid processing unit is integrally moulded forming preferably.
In above-mentioned fluid processing unit, when the amount of liquid when be fed to the fluid containment part from the container body openings in is not more than scheduled volume, by being applied to the capillary force in the first fluid container cavity and being applied to the poor of capillary force in the second fluid containment chamber, communication passage preferably causes the liquid in the first fluid container cavity to enter into the second fluid containment chamber, and stops the second fluid containment intracavity liquid to enter the first fluid container cavity.In this case, be applied to capillary force in the second fluid containment chamber preferably greater than the capillary force that is applied in the first fluid container cavity.
According to a further aspect in the invention, a kind of fluid treating device comprises: device body; And a plurality of fluid processing units that are arranged on the device body, wherein, each unit in a plurality of fluid processing units is exactly above-mentioned fluid processing unit.
In this fluid treating device, a plurality of fluid processing units preferably are arranged to matrix at device body.In this case, a plurality of fluid processing units are together with device body molded forming integratedly.Device body preferably comprises framework and a plurality of supporting member that is arranged on the framework substantially parallelly, and a plurality of fluid processing units preferably are arranged to a row with equidistant spacing at each supporting member.In this case, a plurality of fluid processing units can integrally mouldedly form together with each supporting member.
According to the present invention, can provide a kind of for the fluid processing unit in the fluid treating device and the fluid treating device that uses this unit, when this device was used as the sample analyzer of measuring a large amount of samples, this unit can improve reaction efficiency and measurement sensitivity and shorten the reaction time and Measuring Time with simple structure.
Also can allow fluid processing unit or use the fluid treating device of this fluid processing unit further to improve analysis precision, the reactant or the sample size that namely are used in analysis are considerably less, also can reach high precision.
Description of drawings
From the accompanying drawing of the following detailed description that provides and the preferred embodiment of the present invention, will understand the present invention more completely.Yet accompanying drawing is not intended to limit the invention to specific embodiment, and accompanying drawing just is used for explaining and being convenient to understanding.
In the accompanying drawings:
Fig. 1 is the stereographic map according to the preferred embodiment of fluid treating device of the present invention;
Fig. 2 is a stereographic map, and framework and the supporting member of the device body of Fig. 1 fluid treating device is shown;
Fig. 3 is the amplification view of supporting member of the fluid processing unit of Fig. 2;
Fig. 4 is the sectional view along Fig. 3 center line IV-IV intercepting;
Fig. 5 is the stereographic map that a certain state is shown, and fluid processing unit is installed on the fluid processing unit supporting member of Fig. 2;
Fig. 6 is the amplification view of one of them fluid processing unit, and each fluid processing unit is installed in of correspondence of Fig. 1 fluid treating device and installs in the sunk part;
Fig. 7 is the sectional view along Fig. 6 center line VII-VII intercepting;
Fig. 8 A is the amplification view of one of fluid processing unit of Fig. 1 fluid treating device;
Fig. 8 B is the sectional view along Fig. 8 A center line VIIIB-VIIIB intercepting;
Fig. 8 C is the sectional view along Fig. 8 B center line VIIIC-VIIIC intercepting;
Fig. 8 D is the enlarged drawing of Fig. 8 C part;
Fig. 9 A is a planimetric map that amplifies, and a spot of liquid is shown is fed to according to the state in the preferred embodiment of fluid processing unit of the present invention, and it is corresponding to Fig. 8 A;
Fig. 9 B is sectional view, a spot of liquid is shown is fed to according to the state in the preferred embodiment of fluid processing unit of the present invention, and it is corresponding to Fig. 8 B;
Figure 10 A is the amplification view of the modification example of fluid processing unit shown in Fig. 8 A to 8D;
Figure 10 B is the sectional view along Figure 10 A center line XB-XB intercepting;
Figure 11 is the chart that shows absorptivity result measured in example and the comparison example;
Figure 12 is the stereographic map of the modification example of the fluid treating device according to the present invention.
Embodiment
Referring now to accompanying drawing, will describe the fluid treating device according to fluid processing unit of the present invention and this unit of use below in detail.
Fig. 1 to 9B shows the preferred embodiment according to fluid processing unit of the present invention and fluid treating device.For example, the fluid treating device 10 in the preferred embodiment can be used as to analyze the device that contains biological substance sample such as protein, and described biological substance has represented functional material.In general, fluid treating device 10 can be used as the sample analyzer that is referred to as little well plate, and it can implement to measure to a large amount of samples.As shown in Figure 1, fluid treating device 10 comprises: device body 12; And a plurality of fluid processing units 16 (being the individual fluid processing units in 96 (=8 * 12) in the preferred embodiment), they are installed in and are arranged to a matrix on the device body 12.
As illustrated in fig. 1 and 2, device body 12 is made by resin material or glass material such as polystyrene (PS), polycarbonate (PC) or polymethylmethacrylate (PMMA), and comprise: the framework 11 of essentially rectangular, the center of framework has the through hole 11a of essentially rectangular, frame thickness is several millimeter, the length of framework 11 each side at several centimetres within the cm range more than ten; And a plurality of fluid processing unit supporting members 13 (being 12 fluid processing unit supporting members in this preferred embodiment) that are installed on the framework 11.In addition, the through hole 11a of framework 11 can replace with the depressed part branch that has the bottom.Perhaps, framework 11 can be standard card cage, for example is used for the framework of the microplate of SBS (biomolecule sieve association) standard.Fluid processing unit supporting member 13 can be made by transparent material.Yet if the fluid treating device in the preferred embodiment 10 is used for measuring fluorescence, fluid processing unit supporting member 13 is preferably made (for example, the black member) with the member that light is difficult to pass through, to suppress the appearance of background in measuring fluorescence process.
As shown in Figure 2, each fluid processing unit supporting member 13 comprises: elongated supporting member body 13a, and it has the roughly shape of rectangular parallelepiped, and its length is substantially equal to the width of framework 11 through hole 11a; And the rectangular outshot 13b basically that highlights of a pair of top from supporting member body 13a, they extend along the upper surface of supporting member body 13a along the longitudinal direction at the place, two ends.As shown in Figure 1, the supporting member body 13a of fluid processing unit supporting member 13 is inserted in the through hole 11a of framework 11, so that substantially parallelly be installed on the framework 11 located adjacent one anotherly, like this, outshot 13b is bearing in along the longitudinal direction on a pair of upper surface 11b of the framework 11 that extends.Therefore, assembling lifting device body 12.
Shown in Fig. 3 and 4, a plurality of diameters and the degree of depth are that the sunk part 14 (being eight sunk parts 14 in this preferred embodiment) (hereinafter will be called " sunk part 14 is installed ") of several millimeters substantial cylindrical is formed in the supporting member body 13a upper surface of each fluid processing unit supporting member 13, are arranged to a row with equidistant interval.Install in the sunk part 14 at each, one of them fluid processing unit 16 is installed as illustrated in fig. 5.
Fig. 6 to 9B is enlarged detailed, and one of them fluid processing unit 16 is shown, and in this preferred embodiment, each fluid processing unit is installed in the correspondence depression of installation sunk part 14 of fluid treating device 10.Fig. 6 is the planimetric map of one of fluid processing unit 16, and each fluid processing unit is installed in the counterpart of installation sunk part 14 of fluid treating device 10, and Fig. 7 is the sectional view along Fig. 6 center line VII-VII intercepting.Fig. 8 A is the planimetric map of one of fluid processing unit 16 of fluid treating device 10 in the preferred embodiment, and Fig. 8 B is the sectional view along Fig. 8 A center line VIIIB-VIIIB intercepting.Fig. 8 C is the sectional view along Fig. 8 B center line VIIIC-VIIIC intercepting, and 8D is the enlarged detail of Fig. 8 C part.Fig. 9 A and 9B illustrate small amount of liquid and are fed to state in the fluid processing unit 16, and Fig. 9 A is the planimetric map corresponding to Fig. 8 A, and Fig. 9 B is the sectional view corresponding to Fig. 8 B.
Each fluid processing unit 16 is made by the resin material such as polystyrene (PS), polycarbonate (PC) or polymethylmethacrylate (PMMA).Shown in Fig. 6 to 8B, each fluid processing unit 16 has the height identical with the degree of depth of corresponding installation sunk part 14 basically, and comprise the cylindrical part 16a of big overall diameter and the cylindrical part 16b of little overall diameter, and interior cylindrical part 16c, they are integratedly molded and integrally formed with each other.
The cylindrical part 16a of big overall diameter is such cylindrical part basically, and it has half height corresponding to a fluid processing unit 16, and its overall diameter is substantially equal to a corresponding interior diameter that sunk part 14 is installed.The cylindrical part 16a of big overall diameter is designed to fit into corresponding one and installs in the sunk part 14, so that being inserted into corresponding one at each fluid processing unit 16 installs in the sunk part 14 and when being installed in wherein, cylindrical part 16a is fixed on the installation sunk part 14.The bottom part of the cylindrical part 16a of big overall diameter is curved and towards interior downward-sloping extending to the cylindrical part 16b of little overall diameter, thereby is connected to the upper end of the cylindrical part 16b of little overall diameter.
The cylindrical part 16b of little overall diameter is such cylindrical part basically, and it has half height corresponding to a fluid processing unit 16, and its overall diameter is less than the overall diameter of the cylindrical part 16a of big overall diameter.The cylindrical part 16b edge of the little overall diameter axial direction identical with the cylindrical part 16a of big overall diameter extends.The bottom part of the cylindrical part 16b of little overall diameter has towards interior downward-sloping part.From this bottom towards interior downward-sloping part, bottom surface portions extends along the direction of the axial direction of the cylindrical part 16b that is substantially perpendicular to little overall diameter.The bottom side of the bottom surface portions of the cylindrical part 16b of little overall diameter has sunk part 16e, the interior diameter of cylindrical part 16c in the diameter of this sunk part is substantially equal to.
Interior cylindrical part 16c is such cylindrical part basically, and its upper surface along the axial direction identical with the cylindrical part 16b of little overall diameter from the bottom surface portions of the cylindrical part 16b of little overall diameter extends upward.The height of interior cylindrical part 16c upper end is lower than the top of the cylindrical part 16b of little overall diameter, and the overall diameter of interior cylindrical part 16c is less than the interior diameter of the cylindrical part 16b of little overall diameter.Interior cylindrical part 16c has a plurality of slit 16d (eight slit 16d in this preferred embodiment), they basically in parallel with each other from the bottom substantial linear of interior cylindrical part 16c extend to its upper end.A plurality of slit 16d arrange equidistantly by interior cylindrical part 16c and along its circumferencial direction.The width of each slit 16d from several microns to the hundreds of micron, and the width of each the slit 16d on interior cylindrical part 16c inside surface one side is greater than the width on its outside surface one side.
In addition, in the cylindrical part 16a of big overall diameter, formation one is used as the space of injection portion 26, and this injection portion 26 is used for injecting the fluid such as liquor sample.Between the cylindrical part 16b and interior cylindrical part 16c of little overall diameter, form outer fluid container cavity 28 (for example, having the volume that is not more than about 30ul), it is the general toroidal space that can be used as reaction chamber.In interior cylindrical part 16c, form inner fluid container cavity 30, it is to can be used as the substantial cylindrical chamber of measuring the chamber.
If a spot of (for example, being not more than about 30ul) is fed in the injection portion 26 such as the such liquid of reactant, then this liquid is fed in one of inner fluid container cavity 30 and outer fluid container cavity 28 or both.(liquid level that is raised up by capillary force) Z can be expressed as Z=2Tcos θ/γ * r * g (θ: contact angle because capillary action rises, T: surface tension, γ: fluid density, r: capillary action radius, g: acceleration of gravity), so, acting on that the capillary force on the liquid is greater than acting on the capillary force on the liquid in the inner fluid container cavity 30 in the outer fluid container cavity 28, described outer fluid container cavity 28 width radially is less than the diameter of inner fluid container cavity 30.Therefore, shown in Fig. 9 A and 9B, be fed in the injection portion 26 most of liquid since capillary action to be sucked into outer fluid container cavity 28 interior and remain in the outer fluid container cavity 28 shown in Reference numeral 32.Therefore, can determine the width W 1 of each the slit 16b in the cylindrical part 16c in being formed on suitably, and the width W 2 of general toroidal outer fluid container cavity 28 (between the interior diameter of the cylindrical part 16b of little overall diameter and the overall diameter of interior cylindrical part 16c poor), like this, the most of liquid that is fed in the injection portion 26 is sucked in the outer fluid container cavity 28.
In addition, most of fluid accumulation in being fed to injection portion 26 outside in the fluid containment chamber 28 after, if make total amount of liquid (for example surpass scheduled volume by liquid additionally being fed in the injection portion 26, about 30ul), then liquid flows in the interior cylindrical part 16c by opening and/or the slit 16d on interior cylindrical part 16c top, like this, liquid can be filled in the outer fluid container cavity 28 and the inside of interior cylindrical part 16c to extend in fully in the fluid processing unit 16.
Therefore, according to the fluid processing unit 16 in this preferred embodiment, if the small amount of liquid such as reactant is fed in the injection portion 26, the most of liquid that then is fed in the injection portion 26 is sucked in the outer fluid container cavity 28, and along the circumferential direction flows in order to be held in the outer fluid container cavity 28 in the fluid containment chamber 28 outside.Therefore, even being used as reaction chamber, outer fluid container cavity 28 comes test sample by a small amount of reactant, also can improve liquid level widely increases the surface area on reaction wall surface (inner wall surface of outer fluid container cavity 28), and also can reduce the distance between sample and the reaction wall surface.Therefore, it can improve reaction efficiency and shorten the reaction time, and can reduce to be used as the quantity of reactant and reduce cost.
According to the fluid processing unit 16 in this preferred embodiment, the reaction volume that namely is used in analysis is considerably less, and this reactant also can stably remain in the outer fluid container cavity 28 that is used as reaction chamber, like this, can further improve analysis precision.In addition, if can be considerably less and make the sample concentration in the solution that contains sample very low for sample size, then because the sample in the solution can not arrive the reactive moieties of borehole wall surface, just there be traditional little well plate can not obtain some situation of stability analysis result.Yet the fluid processing unit 16 in this preferred embodiment can stably be fed to sample in the outer fluid container cavity 28 of making reaction chamber, like this, compares with the little well plate of tradition, just can further improve analysis precision.
According to the fluid processing unit 16 in this preferred embodiment, for reactant being fed to outer fluid container cavity 28, even reactant is not to present along injection portion 26 inwalls, is fed to reactants in the inner fluid container cavity 30 from injection portion 26 and also can be sucked in the outer fluid container cavity 28 and maintains wherein.Therefore, no matter the reactant feed position how, reactant can automatically move in the outer fluid container cavity 28 and be maintained at wherein, like this, can easily present implementation and operation to reactant.
In addition, the fluid processing unit 16 in this preferred present embodiment, if the width of each the slit 16d on interior cylindrical part 16c inner face one side greater than the width on its outside one side, then the liquid levels in the outer fluid container cavity 28 can keep smooth basically.In addition, even be fed to the amount of liquid such as reactant very little (being not more than the volume of outer fluid container cavity 28) in the injection portion 26, also can be between a plurality of fluid processing units 16 and between the measurement operation, the variation of the liquid area of the inner wall surface of inhibition contact outer fluid container cavity 28.
In addition, according to the fluid processing unit 16 in this preferred embodiment, be fed in the injection portion 26 and be filled in fluid processing unit 16 inside (inside of injection portion 26, outer fluid container cavity 28 and inner fluid container cavity 30) afterwards at the clean solution of q.s, can easily discharge this clean solution.Therefore, the fluid processing unit 16 in this preferred embodiment has outstanding sanitary characteristics, and can reduce background in measuring process.In addition, because the height of interior cylindrical part 16c upper end is lower than the height of the cylindrical part 16a upper end of big overall diameter, so, the clean solution of q.s can be fed in the injection portion 26, so that the composition that will remove floats, like this, just can discharge these compositions by means of the device of pipette and so on.Therefore, the situation of height that equals the cylindrical part 16a upper end of big overall diameter with the height of interior cylindrical part 16c upper end is compared, and the fluid processing unit 16 in this preferred embodiment has more outstanding sanitary characteristics.
In addition, the fluid processing unit 16 in this preferred embodiment can form with methods such as injection moulding are integrally moulded, and it can easily be made.As the example of a modification of the fluid treating device 10 in this preferred embodiment, can be with the integrally moulded formation supporting member 13 of methods such as injection moulding, so that a plurality of fluid processing unit 16 is arranged in into a row equidistantly.Perhaps, as shown in figure 12, the device body 212 of plate shape can form with methods such as injection moulding are integrally moulded, so that a plurality of fluid processing unit 16 is arranged to matrix, and need not to provide the supporting member of any fluid processing unit.
Figure 10 A and 10B illustrate a kind of modification example of the fluid processing unit 16 in this preferred embodiment.Fluid processing unit 116 in this modification example has the structure identical with fluid processing unit 16 structures, exception be that the upper surface of interior cylindrical part 116c is towards interior downward-sloping.Therefore, the Reference numeral that those structure divisions identical with fluid processing unit 16 structure divisions are given is added 100, to omit it is repeated in this description.If the upper surface of interior cylindrical part 116c towards interior downward-sloping and form as this modification example inclined surface 116f, then when liquid being fed in the fluid processing unit 116 by means of pipette, the upper end of cylindrical part 116c in even the end portion of pipette collides, the end portion of pipette also can be guided in the inner fluid container cavity 130 glossily.Therefore, can prevent in cylindrical part 116c collide distortion and the breakage that causes because of pipette and interior cylindrical part 116c.
As a kind of example of the fluid processing unit 16 in this preferred embodiment, below description is used as an example of the fluid processing unit of sample analysis unit.
At first, anti-TNF-Alpha antibodies of 100ul is fed in the injection portion 26 of fluid processing unit 16, kept two hours down at 25 ℃, on the inwall of fluid processing unit 16, to capture (or catching) antibody immovably.After this, (the prominent literary composition of PBS-0.02% (Tween) 20) is fed to injection portion 26 with the clean solution of 170 μ l, then, and with its discharge and cleaning fluid processing unit 16 inside.
Then, be fed to injection portion 26 and kept 16 hours down and after blocking the inwall of fluid processing unit 16 at 4 ℃ at the obstruction solution (PBS-1%BSA) with 170 μ l, but venting should block solution.
Then, the TNF-Alpha antibodies with 100 μ l is fed to injection portion 26 and causes antigen-reactive (example reaction) 25 ℃ of following maintenances 1 hour.After this, the clean solution (PBS-0.02%Tween20) of 170 μ l is fed to injection portion 26, then, with its discharge and cleaning fluid processing unit 16 inside.
Then, the antibody that indicates biotin of 100ul is fed to injection portion 26 and kept 1 hour down and cause the detections of antagonist reaction at 25 ℃, after this, the clean solution (PBS-0.02%Tween20) of 170 μ l is fed to injection portion 26, then, with its discharge and cleaning fluid processing unit 16 inside.
Then, the enzyme (HRP peroxidase streptavidin) of 100 μ l is fed to injection portion 26 and causes enzyme reaction 25 ℃ of following maintenances 20 minutes, after this, the clean solution (PBS-0.02%Tween20) of 170 μ l is fed to injection portion 26, then, with its discharge and cleaning fluid processing unit 16 inside.
Then, the nutrient culture media (TMB) of 50 μ l is fed to injection portion 26 and kept 10 minutes down and cause the nutrient culture media reaction at 25 ℃, then, the reaction of 50ul is stopped solution (1N HCl) be fed to injection portion 26 to stop reaction.Then, be light (vertical direction) irradiation inner fluid container cavity 30 along the longitudinal direction of 450nm with wavelength, to measure the reaction solution absorption intensity in the inner fluid container cavity 30.
As a comparative example, have with this preferred embodiment in the identical shaped substantial cylindrical well of the installation sunk part 14 of fluid treating device 10 be used to the measurement that realizes that this is identical.
Its result, as can be seen from Fig. 11, in the example of the fluid processing unit 16 that adopts this preferred embodiment, its absorptivity be comparative example absorptivity twice or more than the twice.Therefore, even amount of liquid (is captured or the quantity of (catching) antibody, antigen as sample, detect antibody etc.) be substantially equal to the amount of comparative example, also can improve measured intensity widely, even amount of liquid far fewer than the quantity of comparative example, also can obtain to be substantially equal to the measured intensity of comparative example quantity.
Although described the present invention for the ease of understanding the present invention better by means of preferred embodiment, should be realized that the present invention can implement in every way and not break away from principle of the present invention.Therefore, the present invention is understood to include all possible embodiment and to the various modifications of illustrated embodiment, implements these embodiment and can not break away from the principle of being set forth by attached claims of the present invention.
Claims (14)
1. fluid processing unit comprises:
Vessel, described vessel have for the bottom and the sidepiece that wherein form the fluid containment part, and described vessel has opening in the top;
Partition wall portions, described partition wall portions is extended from described bottom, is used for the described fluid containment of described vessel partly is separated into first fluid container cavity and the second fluid containment chamber; And
Communication passage, described communication passage are communicated with in order to set up between described first fluid container cavity and the described second fluid containment chamber by described partition wall portions,
Wherein, described communication passage links to each other with the described first and second fluid containment chambeies, be used for being fed to the amount of liquid of described fluid containment in partly when being not more than scheduled volume when the described opening from described vessel, make liquid in the described first fluid container cavity because capillary action enters into the described second fluid containment chamber, simultaneously stop the liquid in the described second fluid containment chamber to enter described first fluid container cavity again, and the amount of liquid in the described opening from described vessel is fed to described fluid containment part then allows the liquid in the described second fluid containment chamber to enter described first fluid container cavity during above scheduled volume; The height of described partition wall portions is lower than the height of the described sidepiece of described vessel;
Described communication passage comprises one or more slits, and described slit is by described partition wall portions, and extends to its upper end from the bottom of described partition wall portions;
Be applied to capillary force in the described second fluid containment chamber greater than the capillary force that is applied in the described first fluid container cavity.
2. fluid processing unit as claimed in claim 1 is characterized in that, described first fluid container cavity is surrounded by the described second fluid containment chamber.
3. fluid processing unit as claimed in claim 2 is characterized in that, described vessel has the substantial cylindrical shape, and described partition wall portions has and described vessel coaxial substantial cylindrical shape roughly.
4. fluid processing unit as claimed in claim 3, it is characterized in that, described vessel has the major diameter part of substantial cylindrical and the small diameter portion of substantial cylindrical, the small diameter portion of described substantial cylindrical is arranged in the major diameter part below of described substantial cylindrical, and described partition wall portions is arranged in the small diameter portion of described substantial cylindrical.
5. fluid processing unit as claimed in claim 3 is characterized in that, described communication passage comprises a plurality of slits, and described slit is arranged with equidistant intervals along the circumferencial direction of described partition wall portions.
6. fluid processing unit as claimed in claim 2 is characterized in that, the upper end of described partition wall portions has towards interior downward-sloping inside surface.
7. fluid processing unit as claimed in claim 1, it is characterized in that, the bottom surface portions in the described second fluid containment chamber being along with from the reducing of distance of described first fluid container cavity and downward-sloping, and the height of the lowermost part of the described bottom surface portions in the described second fluid containment chamber is substantially equal to the height of lowermost part of the bottom surface portions of described first fluid container cavity.
8. fluid processing unit as claimed in claim 1 is characterized in that, each described slit at the width on described that side of first fluid container cavity greater than the width on described second that side of fluid containment chamber.
9. fluid processing unit as claimed in claim 1 is characterized in that, described fluid processing unit is integrally moulded forming.
10. fluid treating device comprises:
Device body; And
A plurality of fluid processing units that are arranged on the described device body,
Wherein, each unit in described a plurality of fluid processing unit is exactly fluid processing unit as claimed in claim 1.
11. fluid treating device as claimed in claim 10 is characterized in that, described a plurality of fluid processing units are arranged to matrix at described device body.
12. fluid treating device as claimed in claim 10 is characterized in that, described a plurality of fluid processing units are together with described device body molded forming integratedly.
13. fluid treating device as claimed in claim 10, it is characterized in that, described device body comprises framework and a plurality of supporting member that is arranged on the described framework substantially parallelly, and described a plurality of fluid processing units are arranged to a row with equidistant spacing at each described supporting member.
14. fluid treating device as claimed in claim 13 is characterized in that, described supporting member is integrally moulded forms together with each for described a plurality of fluid processing units.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007-137101 | 2007-05-23 | ||
| JP2007137101 | 2007-05-23 | ||
| JP2007137101 | 2007-05-23 | ||
| JP2008009997 | 2008-01-21 | ||
| JP2008009997A JP5070069B2 (en) | 2007-05-23 | 2008-01-21 | Fluid handling unit and fluid handling apparatus using the same |
| JP2008-009997 | 2008-01-21 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101311727A CN101311727A (en) | 2008-11-26 |
| CN101311727B true CN101311727B (en) | 2013-08-28 |
Family
ID=40100450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810108740 Expired - Fee Related CN101311727B (en) | 2007-05-23 | 2008-05-23 | Fluid handling unit and fluid handling apparatus using same |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP5070069B2 (en) |
| CN (1) | CN101311727B (en) |
| AT (1) | ATE544518T1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8342041B2 (en) * | 2009-07-15 | 2013-01-01 | Protedyne Corporation | Tube for separating portions of a sample |
| CN108722504A (en) * | 2017-04-19 | 2018-11-02 | 光宝电子(广州)有限公司 | Detection device and its injection mouth structure |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6083761A (en) * | 1996-12-02 | 2000-07-04 | Glaxo Wellcome Inc. | Method and apparatus for transferring and combining reagents |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09101302A (en) * | 1995-10-06 | 1997-04-15 | Toppan Printing Co Ltd | Microplate |
| JPH09159673A (en) * | 1995-12-12 | 1997-06-20 | Toppan Printing Co Ltd | Microplate |
| ES2115521B1 (en) * | 1996-02-26 | 1999-02-16 | Grifols Grupo Sa | DEVICE FOR THE CONDUCT OF ERITROCITAR REACTIONS. |
| JP3584616B2 (en) * | 1996-07-08 | 2004-11-04 | 凸版印刷株式会社 | Microplate |
| JP2001004628A (en) * | 1999-06-18 | 2001-01-12 | Kanagawa Acad Of Sci & Technol | Immunoassay and its method |
| EP1547686A1 (en) * | 2003-12-22 | 2005-06-29 | F.Hoffmann-La Roche Ag | Microtiter plate, system and method for processing samples |
| JP3805352B1 (en) * | 2005-05-25 | 2006-08-02 | 株式会社エンプラス | Fluid handling device and fluid handling unit used therefor |
| JP4680037B2 (en) * | 2005-11-14 | 2011-05-11 | 株式会社エンプラス | Fluid handling device and fluid handling unit used therefor |
| JP4842788B2 (en) * | 2006-03-16 | 2011-12-21 | 株式会社エンプラス | Fluid handling device and fluid handling unit used therefor |
-
2008
- 2008-01-21 JP JP2008009997A patent/JP5070069B2/en not_active Expired - Fee Related
- 2008-05-16 AT AT08009104T patent/ATE544518T1/en active
- 2008-05-23 CN CN 200810108740 patent/CN101311727B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6083761A (en) * | 1996-12-02 | 2000-07-04 | Glaxo Wellcome Inc. | Method and apparatus for transferring and combining reagents |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE544518T1 (en) | 2012-02-15 |
| JP2009002927A (en) | 2009-01-08 |
| CN101311727A (en) | 2008-11-26 |
| JP5070069B2 (en) | 2012-11-07 |
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