CN101306019B - Hyper-concentrated placenta nano viable cell freeze-drying capsules and its preparation method - Google Patents
Hyper-concentrated placenta nano viable cell freeze-drying capsules and its preparation method Download PDFInfo
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Abstract
The invention provides a preparation method of hyperconcentration placental-peptide nanometer viable-cell freeze-dried powder, and a hyperconcentration placental-peptide nanometer viable-cell freeze-dried capsule which comprises the hyperconcentration placental-peptide nanometer viable-cell freeze-dried powder prepared by the preparation method. The preparation method adopts the CO2 supercritical extraction, the ultra-low-temperature freeze-drying technology, and the biological nanotechnology. The hyperconcentration placental-peptide nanometer viable-cell freeze-dried capsule is obtained by utilizing the lipidosome preparation technology.
Description
Technical field
The hyper-concentrated placenta nano viable cell freeze-drying capsules that the present invention relates to a kind of method for preparing of hyper-concentrated placenta nano viable cell freeze-drying powder and comprise it.This hyper-concentrated placenta nano viable cell freeze-drying capsules is processed through liposome technology.
Background technology
Placenta Hominis Chinese medicine is called Placenta Hominis, have regulate function of human body, build up resistance, the effect of strongly invigorating primordial QI essence and blood.Placenta extract is the medicinal ingredient that from Placenta Hominis, extracts, and contains multiple hormone and enzyme.The spissated method of steaming and decocting is adopted in the extraction of Placenta extract in the past more.The inventor is through research; A kind of method of utilizing carbon dioxide supercritical fluid extraction to extract Placenta extract is provided; See also Chinese patent 200410096974.0; The active component that the Placenta extract that utilizes this method to extract has in the Placenta extract that is extracted can not run off, and the Placenta extract in the Placenta Hominis extracts advantage more completely.
The Placenta extract that the inventor will utilize carbon dioxide supercritical extraction method to extract has been processed injection; Injection is painful, application risk is higher to being brought by the injection people but the injection product has; The shortcoming of being inconvenient to carry, and some the active gene regulatory factors and the part metabolic regulation enzyme that utilized digesting technoloy method acquisition Placenta extract to have in the Placenta Hominis originally can be because of the shortcomings of high temperature action inactivation.Therefore, for providing a kind of being more convenient for to use, injection is not painful, application risk is low, is convenient for carrying, and can fully keeps the Placenta extract product of active gene regulatory factor and metabolic regulation enzyme to have extensively and exigence.
In addition; Utilize Co 2 supercritical fluid low-temperature extraction technology (SFE) though on the extraction separation level of Placenta extract, obtained than quantum jump; Use the composition of the Placenta Hominis of this technology extraction to improve more than 1100 times than traditional handicraft; And its molecular weight that extracts composition is littler than traditional method, be more conducive to absorption of human body, and this technology great advantage is can be almost 100% to keep the physiologically active that extracts composition.But, utilize the Placenta extract of SFE technology " the active extraction " also to exist the molecular weight of product not enough little, the Placenta extract nucleus that human body finally absorbs fusion does not still reach the problem of very high level.
Therefore; The still bigger problem of molecular weight to the Placenta extract that utilizes the SFE technology to extract at present; The inventor provides a kind of Placenta extract that utilizes biological nano technology (bionanotechnicology) to extract, and the nucleus of this Placenta extract is made into the ultramicro powder that particle diameter is 1～100nm.Experiment showed, and utilize the placenta nano granule of biological nano technology preparation to have significant raising in the following areas than existing Placenta extract:
1. improve the dissolubility and the biological effectiveness of Placenta extract;
2. strengthen former powerfully, increase new effect;
3. promote traditional route of administration;
4. improve the interaction property of Placenta extract and human body protein.
Summary of the invention
In order to realize this purpose, one aspect of the present invention provides a kind of method of utilizing carbon dioxide supercritical fluid extraction method and cryogenic temperature freezing drying method for distilling to prepare the hyper-concentrated placenta nano viable cell freeze-drying powder.
Another aspect of the present invention provides a kind of hyper-concentrated placenta nano viable cell freeze-drying capsules that utilizes carbon dioxide supercritical fluid extraction method and the preparation of cryogenic temperature freezing drying method for distilling, and this hyper-concentrated placenta nano viable cell freeze-drying capsules is processed through liposome technology.
The invention provides a kind of method for preparing of hyper-concentrated placenta nano viable cell freeze-drying powder, comprising:
A) Placenta Hominis is passed through lyophilization 5～6 hours under-5 ℃～-6 ℃ condition, be developed into powder;
B) the Placenta Hominis powder that obtains is packed in the extraction kettle, feed the carbon dioxide of 30～40MPa, under 30～40 ℃ condition, extract;
C) carry out second depressurized and separate, obtain Placenta extract, one-level decompression separation condition is operating pressure 8～12MPa, and temperature is 30～35 ℃; The second depressurized separation condition be operating pressure at 6MPa, temperature is 15～25 ℃;
D) will utilize Placenta extract product homogenate in sterilized water under aseptic condition of second depressurized resulting separation, and utilize centrifuge centrifugal, centrifugal condition is 3500～4000rmp; Filter, obtain first clear liquid;
E) first clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains first ultrafiltrate, and this first ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in 1～100nm scope;
F) first ultrafiltrate of resulting particle diameter in 1～100nm scope carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
Alternatively, this method also comprises:
The high-molecular weight macromole product that h) will be trapped shifts out, utilize restricted or half restricted enzyme under 30～40 ℃ to Placenta extract in the nucleic acid or the polypeptide of macromolecule carry out the reaction of Restriction Enzyme inscribe;
The Placenta extract product that i) will after the reaction of Restriction Enzyme inscribe, obtain utilizes centrifuge centrifugal under aseptic condition, and centrifugal condition is 3500～4000rmp; Filter, obtain second clear liquid;
J) second clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains second ultrafiltrate, and this second ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in the scope of 1～100nm;
K) resulting second ultrafiltrate is carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
Preferably, further comprising: g) will be by step f) or step k) the nanometer Placenta extract lyophilized powder of the said ultra concentration that obtains is packaged in the capsule coating by certain UD under aseptic condition, obtains hyper-concentrated placenta nano viable cell freeze-drying capsules.
Preferably, capsule of the present invention utilizes liposome technology to make.
Preferably; Capsule coating of the present invention is selected from by polyvinyl alcohol, modification of chitosan, gelatin, sodium alginate, hydroxypropyl cellulose, methylcellulose, hydroxypropyl emthylcellulose, hydroxyethyl-cellulose, polyvinylpyrrolidone, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl acetal-lignocaine acetate copolymer, diethyllaminoethyl acrylic acid methyl ester .-methylmethacrylate copolymer, cellulose acetate-phthalate; Hydroxypropyl Methylcellulose Phathalate, hexahydrophthalic acid cellulose acetate, hexahydrophthalic acid hydroxypropyl emthylcellulose, PMA, polyethyl acrylate, butyl polyacrylate, polymethyl methacrylate, polyethyl methacrylate, butyl polyacrylate, PHEMA, poly hydroxy ethyl acrylate, polyacrylic acid hydroxypropyl acrylate, polymethylacrylic acid hydroxypropyl acrylate, polyacrylic acid 4-hydroxy butyl ester, polymethylacrylic acid 4-hydroxy butyl ester, and in the group of the copolymer of above material or mixture formation.
Preferably, capsule coating of the present invention be gastric solubility or enteric solubility.
The present invention also provides a kind of hyper-concentrated placenta nano viable cell freeze-drying capsules that utilizes method for preparing, comprises the hyper-concentrated placenta nano viable cell freeze-drying powder and the capsule coating that are made by above-mentioned hyper-concentrated placenta nano viable cell freeze-drying powder, preparation method thereof.
Preferably, capsule of the present invention is processed through liposome technology.
Preferably, the restricted enzyme that the present invention adopts is Mmel, TCCRAC (20/18); Eco57I, CTGAAG (16/14); Eco57MI, CTGRAG (16/14); Or Bce83I, CTTGAG (16/14).Wherein, Mmel, TCCRAC (20/18) are meant that the segment of Mmel enzyme identification is TCCRAC, and its restriction enzyme site then is positioned at the 20th nucleotide in these segment downstream; Restriction enzyme site on complementary strand then is positioned at the 18th nucleotide in these segment downstream, and other restricted enzyme has similar implication.
Alternatively, hyper-concentrated placenta nano viable cell freeze-drying capsules of the present invention also comprises pharmaceutical excipient.
The specific embodiment
Followingly according to the specific embodiment the present invention is specifically described, it only is in order more to be expressly understood the present invention, and should not be used for limiting protection scope of the present invention, all are equal to replacement and all should be included in the scope of invention.
In a specific embodiment of the present invention, a kind of method of utilizing carbon dioxide supercritical fluid extraction method and cryogenic temperature freezing drying method for distilling to prepare the hyper-concentrated placenta nano viable cell freeze-drying powder is provided, this method comprises:
A) Placenta Hominis is passed through lyophilization 5～6 hours under-5 ℃～-6 ℃ condition, be developed into powder;
B) the Placenta Hominis powder that obtains is packed in the extraction kettle, feed the carbon dioxide of 30～40MPa, under 30～40 ℃ condition, extract;
C) carry out second depressurized and separate, obtain Placenta extract, one-level decompression separation condition is operating pressure 8～12MPa, and temperature is 30～35 ℃; The second depressurized separation condition be operating pressure at 6MPa, temperature is 15～25 ℃;
D) will utilize Placenta extract product homogenate in sterilized water under aseptic condition of second depressurized resulting separation, and utilize centrifuge centrifugal, centrifugal condition is 3500～4000rmp; Filter, obtain first clear liquid;
E) first clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains first ultrafiltrate, and this first ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in 1～100nm scope;
F) first ultrafiltrate of resulting particle diameter in 1～100nm scope carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
In a preferred embodiment, this method also comprises:
The high-molecular weight macromole product that h) will be trapped shifts out, utilize restricted or half restricted enzyme under 30～40 ℃ to Placenta extract in the nucleic acid or the polypeptide of macromolecule carry out the reaction of Restriction Enzyme inscribe;
The Placenta extract product that i) will after the reaction of Restriction Enzyme inscribe, obtain utilizes centrifuge centrifugal under aseptic condition, and centrifugal condition is 3500～4000rmp; Filter, obtain second clear liquid;
J) second clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains second ultrafiltrate, and this second ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in the scope of 1～100nm;
K) resulting second ultrafiltrate is carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
In another specific embodiment; Further comprising: g) will be by step f) or step k) the nanometer Placenta extract lyophilized powder of the said ultra concentration that obtains is packaged in the capsule coating by certain UD under aseptic condition, obtains hyper-concentrated placenta nano viable cell freeze-drying capsules.
In a specific embodiment, capsule of the present invention utilizes liposome technology to make.
In another specific embodiment; Capsule coating of the present invention is selected from by polyvinyl alcohol, modification of chitosan, gelatin, sodium alginate, hydroxypropyl cellulose, methylcellulose, hydroxypropyl emthylcellulose, hydroxyethyl-cellulose, polyvinylpyrrolidone, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl acetal-lignocaine acetate copolymer, diethyllaminoethyl acrylic acid methyl ester .-methylmethacrylate copolymer, cellulose acetate-phthalate; Hydroxypropyl Methylcellulose Phathalate, hexahydrophthalic acid cellulose acetate, hexahydrophthalic acid hydroxypropyl emthylcellulose, PMA, polyethyl acrylate, butyl polyacrylate, polymethyl methacrylate, polyethyl methacrylate, butyl polyacrylate, PHEMA, poly hydroxy ethyl acrylate, polyacrylic acid hydroxypropyl acrylate, polymethylacrylic acid hydroxypropyl acrylate, polyacrylic acid 4-hydroxy butyl ester, polymethylacrylic acid 4-hydroxy butyl ester, and in the group of the copolymer of above material or mixture formation.
In a specific embodiment, capsule coating of the present invention is a gastric solubility.
And in another specific embodiment, capsule coating of the present invention is an enteric solubility.
In a specific embodiment of the present invention; A kind of nanometer Placenta extract capsule that utilizes the ultra concentration of carbon dioxide supercritical fluid extraction method and the preparation of cryogenic temperature freezing drying method for distilling is provided, and this hyper-concentrated placenta nano viable cell freeze-drying capsules is processed through liposome technology.
In a specific embodiment of the present invention; A kind of hyper-concentrated placenta nano viable cell freeze-drying capsules that utilizes method for preparing is provided, has comprised the hyper-concentrated placenta nano viable cell freeze-drying powder and the capsule coating that make by above-mentioned hyper-concentrated placenta nano viable cell freeze-drying powder, preparation method thereof.
In another specific embodiment, capsule of the present invention is processed through liposome technology.Only if clear and definite qualification is arranged, the disclosed liposome technology of all prior aries can be used in the present invention.
In a preferred embodiment, the restricted enzyme that the present invention adopts is Mmel, TCCRAC (20/18); Eco57I, CTGAAG (16/14); Eco57MI, CTGRAG (16/14); Or Bce83I, CTTGAG (16/14).Wherein, Mmel, TCCRAC (20/18) are meant that the segment of Mmel enzyme identification is TCCRAC, and its restriction enzyme site then is positioned at the 20th nucleotide in these segment downstream; Restriction enzyme site on complementary strand then is positioned at the 18th nucleotide in these segment downstream, and other restricted enzyme has similar implication.
In a specific embodiment, hyper-concentrated placenta nano viable cell freeze-drying capsules of the present invention also comprises pharmaceutical excipient.Only if clear and definite qualification is arranged, the disclosed pharmaceutical excipient of all prior aries needs only the pharmaceutical excipient compatible with hyper-concentrated placenta nano viable cell freeze-drying powder of the present invention and can use in the present invention.
The method for preparing of embodiment 1 hyper-concentrated placenta nano viable cell freeze-drying powder and the capsule that makes thereof
A) with the lyophilization 5～6 hours under-5 ℃～-6 ℃ condition of fresh Placenta Hominis, be developed into powder;
B) the Placenta Hominis powder that obtains is packed in 11 the extraction kettle, feed the carbon dioxide of about 30MPa, under 30～40 ℃ condition, extract;
C) carry out second depressurized and separate, one-level decompression separation condition is operating pressure 8～12MPa, and temperature is 30～35 ℃; The second depressurized separation condition is that operating pressure is about 6MPa, and temperature is 15～25 ℃, thereby obtains Placenta extract;
D) will utilize Placenta extract product homogenate in sterilized water under aseptic condition of second depressurized resulting separation, and utilize centrifuge centrifugal, centrifugal condition is 3500～4000rmp; Filter, obtain first clear liquid;
E) first clear liquid being used molecular cut off is about 3000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains first ultrafiltrate, and this first ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in 1～100nm scope;
F) first ultrafiltrate of resulting particle diameter in 1～100nm scope carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
G) will under aseptic condition, be packaged in the capsule coating of processing by Hydroxypropyl Methylcellulose Phathalate by the said hyper-concentrated placenta nano viable cell freeze-drying powder that step f) obtains, obtain the nanometer Placenta extract capsule of the ultra concentration of enteric solubility by certain UD.
H) molecular weight that is trapped is shifted out greater than 3000 macromole product, utilizes restricted or half restricted enzyme Mmel, TCCRAC (20/18) under 30～40 ℃ to Placenta extract in the nucleic acid or the polypeptide of macromolecule carry out the reaction of Restriction Enzyme inscribe;
The Placenta extract product that i) will after the reaction of Restriction Enzyme inscribe, obtain utilizes centrifuge centrifugal under aseptic condition, and centrifugal condition is 3500～4000rmp; Filter, obtain second clear liquid;
J) second clear liquid being used molecular cut off is about 5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains second ultrafiltrate, and this second ultrafiltrate particle diameter is nanometer Placenta extract ultrafiltrate in the scope of 1～100nm;
K) resulting second ultrafiltrate is carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the nanometer Placenta extract lyophilized powder of ultra concentration.
L) will be by step k) the nanometer Placenta extract lyophilized powder of the said ultra concentration that obtains is packaged in the capsule coating of hydroxyethyl-cellulose by certain UD under aseptic condition, obtains the hyper-concentrated placenta nano viable cell freeze-drying capsules of gastric solubility.
Embodiment 2 concentrates the capsular result of use of placenta nano viable cell
This experiment is that the object of observation is carried out with slight senilism person.
Phase I: take hyper-concentrated placenta nano viable cell freeze-drying capsules by the encapsulation of certain UD after 10～20 days (each one once a day) the experimenter.
In this phase I:
● experimenter's outward appearance shows as: facial glossy, ruddy, radiant, that skin begins is moistening, moisture is arranged, and it is flexible to press skin with hands, and spot is tentatively desalinated, and wrinkle shoals.
● experimenter's physiological performance is: feeling of fatigue is eliminated, muscle power strengthens, full of vitality, appetite increases, sleep improves and have deep sleep, sexual function obviously to strengthen.
Second stage: take hyper-concentrated placenta nano viable cell freeze-drying capsules by the encapsulation of certain UD after 30～50 days the experimenter.
In this second stage:
● experimenter's outward appearance shows as: radiant, the skin quality is obviously improved, and mainly is that skin glossiness, moisture degree, DE significantly increase, shrinking pore, and black eye disappear; Pigment begins to disappear, and facial deep layer wrinkle shoals, progressively full, and tiny wrinkle disappears, and mottle is thin out to disappearing, and the part senile plaque can disappear; Hair restoration, pitch-black shinny, thigh and calf fat reduces, and stomach fat reduces, and figure's profile beautifies; Bung fat reduces, breast enlargement, and chest measurement increases, and reinvents the charm body.
● experimenter's physiological performance is: passive protective physical fitness strengthens, and sleep quality obviously improves, and is energetic, and body constitution is whole to be improved.The patient that takes a disease can occur: climacteric syndrome doing well,improving, disappearance, and arthralgia disappears, and rheumatism is obviously improved, the dizzy person's transference cure of hypertension; Do not have and have palpitation, nothing is breathed hard, and blood pressure is steady, and refractory headache the person improve; Lumbar muscle strain obviously improves, and flu does not recur, and respiratory tract disease is clearly better, and cardiovascular disease takes a turn for the better; Blood pressure, blood pressure and blood lipoid continue to keep normally, and glucose in urine is turned out cloudy, and liver function takes a turn for the better or be normal, and tumor is controlled or dwindled.
Phase III: the experimenter take by the hyper-concentrated placenta nano viable cell freeze-drying capsules 60～80 (or 120) of certain UD encapsulation day and after.
In this phase III:
● experimenter's outward appearance shows as: wrinkle is unfolded disappearance, and mottle disappears, and skin is white touched with red, pale tender, moist flexible, beautiful gloss, and radiant, full of vigor, it is well-balanced that the figure becomes, slow down aging.
The result of the outward appearance performance of experimenter after taking 10 months is summarised in the table 1.
Table 1
* note: occur two or more than be effectively.Because of the difference of individuality, there is difference in this product to the performance of Different Individual.
● experimenter's physiological performance is: body constitution rises comprehensively, and immunologic function significantly improves, and the human body metabolism is normal, and neuroendocrine is coordinated, and gynaecopathia obviously reduces, and is energetic, and human body is full of healthy vigor, and the women of part premature menopause can recover to pass through.
Hyper-concentrated placenta nano viable cell freeze-drying capsules of the present invention; It is to adopt the carbon dioxide supercritical fluid extraction technology to extract; And utilize biological restriction endonuclease technology to carry out the enzyme inscribe reaction of molecule; Making is become nano level Placenta extract molecule by the Placenta extract of enzyme inscribe, and the hyper-concentrated placenta nano viable cell freeze-drying capsules that utilizes cryogenic temperature freezing drying and liposome technology to obtain.
The hyper-concentrated placenta nano viable cell freeze-drying capsules that utilizes the inventive method to obtain has kept the active ingredient of Placenta Hominis to greatest extent, and the capsule that adopts liposome technology to obtain, and more can help the absorption of human body Placenta extract.Thereby improve user's skin, the U.S. breast of strong breast strengthens skin elasticity, promotes hematopoietic function, slow down aging, and human body immunity improving power, hypermnesis, endocrine regulation improves the women's physiological function.
Through check, the hyper-concentrated placenta nano viable cell freeze-drying capsules that obtains through the inventive method contains each seed amino acid, bioactive peptide, mineral, nucleic acid, various protease, the basic element of cell division, vitamin, like VB1, VB2, VB6, VE, VC etc.
The hyper-concentrated placenta nano viable cell freeze-drying capsules taking convenience that utilizes the present invention to obtain; Avoided the shortcoming of injection, be more convenient for using, injection is not painful, application risk is low; Be convenient for carrying, and can fully keep active gene regulatory factor and metabolic regulation enzyme.
Utilize hyper-concentrated placenta nano viable cell freeze-drying powder, preparation method thereof provided by the invention, can:
1, can extract the 8000 various active materials that are rich in the Placenta Hominis, extraction ratio can be up to more than 99%.
But 2, utilize the molecular weight purifies and separates of the Placenta Hominis active ingredient of this method extraction to arrive minimum 3000 dalton;
3, the level of activity and the activity substance content that utilize the ultra concentration nanometer Placenta extract that this method obtains are near the Placenta extract injection;
4, experiment proof: the Placenta extract product that utilizes the activity of the Placenta Hominis composition that this method extracts can utilize carbon dioxide supercritical fluid extraction to obtain more merely improves about 67.73%.
Claims (10)
1. the method for preparing of a hyper-concentrated placenta nano viable cell freeze-drying powder comprises:
A) Placenta Hominis is passed through lyophilization 5～6 hours under-5 ℃～-6 ℃ condition, be developed into powder;
The said Placenta Hominis powder that b) will obtain is packed in the extraction kettle, feeds the carbon dioxide of 30～40MPa, under 30～40 ℃ condition, extracts;
C) carry out second depressurized and separate, obtain Placenta extract, one-level decompression separation condition is operating pressure 8～12MPa, and temperature is 30～35 ℃; The second depressurized separation condition be operating pressure at 6MPa, temperature is 15～25 ℃;
D) separate the Placenta extract product homogenate in sterilized water under aseptic condition that obtains with said through second depressurized, utilize centrifuge centrifugal, centrifugal condition is 3500～4000rmp; Through filtering, obtain first clear liquid;
E) said first clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains first ultrafiltrate, and the particle diameter of said first ultrafiltrate is in 1～100nm scope;
The high-molecular weight macromole product that h) will be trapped shifts out, utilize restricted or half restricted enzyme under 30～40 ℃ to Placenta extract in the nucleic acid or the polypeptide of macromolecule carry out the reaction of Restriction Enzyme inscribe;
I) under aseptic condition, utilize centrifuge centrifugal the said Placenta extract product that after the reaction of Restriction Enzyme inscribe, obtains, centrifugal condition is 3500～4000rmp; Filter, obtain second clear liquid;
J) said second clear liquid being used molecular cut off is 3000～5000 daltonian ultrafilter membrane ultrafiltration 1～2 time, obtains second ultrafiltrate, and the particle diameter of said second ultrafiltrate is in the scope of 1～100nm;
K) said second ultrafiltrate that obtains is carried out cryogenic temperature freezing drying under aseptic condition and handle, obtain the hyper-concentrated placenta nano viable cell freeze-drying powder.
2. method for preparing according to claim 1, wherein said restricted enzyme are Mmel, TCCRAC (20/18); Eco57I, CTGAAG (16/14); Eco57MI, CTGRAG (16/14); Or Bce83I, CTTGAG (16/14).
3. the method for preparing of a hyper-concentrated placenta nano viable cell freeze-drying capsules; Comprising: g) will be by said step k according to the said method for preparing of claim 1) the said hyper-concentrated placenta nano viable cell freeze-drying powder that obtains is packaged in the capsule coating by certain UD under aseptic condition, obtains hyper-concentrated placenta nano viable cell freeze-drying capsules.
4. method for preparing according to claim 3, wherein said capsule coating is an enteric solubility.
5. method for preparing according to claim 3, wherein said capsule coating is a gastric solubility.
6. method for preparing according to claim 3; Wherein said capsule coating is selected from by polyvinyl alcohol, modification of chitosan, gelatin, sodium alginate, hydroxypropyl cellulose, methylcellulose, hydroxypropyl emthylcellulose, hydroxyethyl-cellulose, polyvinylpyrrolidone, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl acetal-lignocaine acetate copolymer, diethyllaminoethyl acrylic acid methyl ester .-methylmethacrylate copolymer, cellulose acetate-phthalate; Hydroxypropyl Methylcellulose Phathalate, hexahydrophthalic acid cellulose acetate, hexahydrophthalic acid hydroxypropyl emthylcellulose, PMA, polyethyl acrylate, butyl polyacrylate, polymethyl methacrylate, polyethyl methacrylate, butyl polyacrylate, PHEMA, poly hydroxy ethyl acrylate, polyacrylic acid hydroxypropyl acrylate, polymethylacrylic acid hydroxypropyl acrylate, polyacrylic acid 4-hydroxy butyl ester, polymethylacrylic acid 4-hydroxy butyl ester, and in the group of the copolymer of above material or admixture formation.
7. hyper-concentrated placenta nano viable cell freeze-drying capsules, said capsule comprise the hyper-concentrated placenta nano viable cell freeze-drying powder and the capsule coating of method preparation according to claim 1 and 2.
8. hyper-concentrated placenta nano viable cell freeze-drying capsules according to claim 7, said capsule further comprises excipient.
9. hyper-concentrated placenta nano viable cell freeze-drying capsules according to the preparation of the method for preparing of each described hyper-concentrated placenta nano viable cell freeze-drying capsules of claim 3～6.
10. hyper-concentrated placenta nano viable cell freeze-drying capsules according to claim 9, said capsule further comprises excipient.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289164C (en) * | 2004-12-07 | 2006-12-13 | 陈包荣 | Carbon dioxide supercritical extraction method for placenta extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1289164C (en) * | 2004-12-07 | 2006-12-13 | 陈包荣 | Carbon dioxide supercritical extraction method for placenta extract |
Non-Patent Citations (1)
| Title |
|---|
| 郭浩华，尤正荣.超滤法浓缩胎盘萃取液的研究.化工时刊.1997,11(10),28-30. * |
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