CN101300958B - 一种间歇浸没式植物培养装置及其控制方法 - Google Patents
一种间歇浸没式植物培养装置及其控制方法 Download PDFInfo
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- CN101300958B CN101300958B CN2007100112628A CN200710011262A CN101300958B CN 101300958 B CN101300958 B CN 101300958B CN 2007100112628 A CN2007100112628 A CN 2007100112628A CN 200710011262 A CN200710011262 A CN 200710011262A CN 101300958 B CN101300958 B CN 101300958B
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
一种用于植物组织液体培养的装置及其控制方法，包括培养箱、灯箱、营养液存储容器；所述培养箱包括工作室、调控仓及控制装置，工作室内部具有液体培养容器、供气管，还设有温/湿度传感器、水位传感器、以及气体浓度传感器；营养液存储容器与液体培养容器之间设有上液总管路及回液总管路，上液总管路上设逆水阀和水泵，回液总管路上设电磁阀；各传感器与控制装置相连；本发明变气体驱动为液体驱动，节省能耗；通过电磁阀的开关调节养分与培养物的接触时间；通过对微环境的调控，提高组培物的质量，使外植体达到最佳的生长、分化和代谢状态，调节气体浓度建立正确的碳的库源关系；整体为箱体结构，不受时间和地点的限制，可以随时随地组织规模化生产。
Description
一种间歇浸没式植物培养装置及其控制方法
技术领域
[0001] 本发明涉及生物技术领域中植物培养的装置及其控制方法，具体地说一种间歇浸没式植物培养装置及其控制方法。
背景技术
[0002] 植物组织培养技术自诞生至今，广泛应用于作物育种、花卉、果树、蔬菜、林木及中草药的脱毒和快繁方面，具有其他技术手段无法比拟的优势，已成为现代生物技术不可或缺的技术领域。为了实现组培高效、低成本工厂化育苗，国内外许多学者就组培技术本身的改进展开了一系列研究，如培养容器和培养基质的替代、非试管苗快繁、无糖培养、液体培养，及最近几年的培养微环境调控等。而高效的商业化快繁依靠的是快速大规模繁殖及组培苗木的高质量。组培程序的机械化和自动化，能有助于其商业化应用。显然，液体培养在实现组培程序的自动化方面首当其选，而且从1994年起，欧盟国家在C0ST822和C0ST843 i 十戈1j (European Cooperation in the field of Scientificand Technical Research) (欧洲科技领域研究合作组织）支持下，连续11年对液体培养、生物反应器技术、适宜材料识别、系统集成以及相关生理学、发育学方面的内容进行了广泛研究，其中有代表性的成果是提出并研制了间歇式气-液交换生物反应器如气升式（RITA® (W096/2M84))和双瓶式 (BIT®)生物反应器，两者的特点是：1)能够保持良好的通风透气，2)连续培养减少了继代次数；幻反应器物化条件可以调控；4)省却难以循环回收的基质物料力）再生植株抗逆性显著增强，从而有效降低了人力物力成本。但气体驱动液体流动使能耗上升、气体驱动不稳定，培养液更新操作复杂，导致环境敏感型（养分和激素）物种发育困难，并且RITA® (the Recipient for Automated TemporaryImmersion System,自云力化间歇浸没系统培养容器） 结构复杂使制造成本过高，BIT® (the Twin flasks system，双瓶式系统）培养瓶冗余使单位空间利用率降低，又限制了它们的普遍应用。
发明内容
[0003] 为了克服上述培养装置结构成本不能控制的缺点，本发明的目的在于提供在自动调控下，改善培养微环境条件基础上，实现液体间歇供应养分，组培植物的增殖和再生的一种用于植物组织液体培养的装置和控制方法。
[0004] 为实现上述目的本发明采用的技术方案是：一种用于植物组织液体培养的装置， 包括：培养箱，为植物组织液体培养的主要装置；灯箱，内部具有照明灯，设于培养箱的上部，对培养箱内部的照明，提供植物所需的光照；营养液存储容器与培养箱连接，为培养箱提供培养植物所需的营养液。
[0005] 所述培养箱包括工作室、调控仓及控制装置，工作室内部具有液体培养容器、供气管，还设有温/湿度传感器、水位传感器、以及气体浓度传感器；供气管通过进气管接至调控仓，液体培养容器与营养液存储容器之间设有上液总管路及回液总管路，上液总管路上设有逆水阀和水泵，回液总管路上设有回液电磁阀；上述各传感器与控制装置相连。[0006] 所述调控仓内具有加湿器水箱，该加湿器水箱底部设有风道，风道外壁上安装有加热/制冷组件；所述风道的进风口通过风机引入空气，出风口经过一电磁阀连接至工作室的供气管中。
[0007] 所述电磁阀为四通电磁阀，其第一进气口与风道的出风口相连，第一出气口通过进气管接至工作室的供气管；第二出气口接回至加湿器的水箱中，四通电磁阀的第二进气口与加湿器的水箱相连通。
[0008] 加湿器水箱内壁上具有光触媒涂层，加湿器水箱内具有发光电极。
[0009] 所述一种装置培养植物组织液体的控制方法，其特征在于具有以下步骤：
[0010] 开机，控制装置初始化； [0011 ] 设定环境变量及控制参数；
[0012] 判断上述控制参数中的控制参数执行时间是否结束；
[0013] 如没有结束，则控制装置采集并显示各传感器检测信号；
[0014] 控制装置将传感器检测信号与控制参数进行比较；
[0015] 如果传感器检测信号没有满足控制参数要求，控制装置输出控制信号启动相应的执行机构进行处理，存储检测信息；
[0016] 如果传感器检测信号满足控制参数要求，存储检测信息；
[0017] 判断控制过程是否结束；
[0018] 如没有结束信息，则返回判断上述控制参数的执行时间是否结束阶段，否则结束控制过程。
[0019] 如果判断上述控制参数的执行时间是否结束的结果为是，则返回至设定控制参数阶段。
[0020] 所述设置控制参数包括具有η套，每套控制参数包括培养植物所需的温度及湿度、所需气体浓度、按植物生长所需光照培养时间、按植物生长所需设置营养液的液位高度及液位保持时间以及每套控制参数执行时间。
[0021] 所述控制装置对传感器检测信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程；
[0022] 其中液位控制过程包括以下步骤：
[0023] 判断液体培养容器内液位是否达到设定高度；
[0024] 如没达到设定高度，则进入高水位时间段，启动水泵给液体培养容器添加培养液至设定高度；
[0025] 判断保持液位高度的保持时间是否达到；
[0026] 如果达到，则打开回水电磁阀，否则继续保持高水位。
[0027] 其中气体浓度控制过程包括以下步骤：
[0028] 判断(X)2气体浓度是否高于设定浓度；
[0029] 如果高于设定浓度，则排出气体；如果低于设定浓度，则吹入(X)2气体。
[0030] 所述温/湿度控制过程包括以下步骤：
[0031] 判断温/湿度是否高于设定温/湿度；
[0032] 如果高于设定温度，则启动制冷组件吹入冷空气；如果低于设定温度，则启动加热组件吹入热空气；[0033] 如果高于设定湿度，则不加湿，通过气体流通降低湿度；如果低于设定湿度则启动加湿装置吹入湿的空气；
[0034] 执行机构包括风机、水泵、加湿器、加热/制冷组件、灯箱以及各电磁阀。
[0035] 光照时间控制过程包括以下步骤：
[0036] 判断是否达到设定的光照时间，如果达到设定的光照时间，则关掉电源；如果未达到设定的光照时间，则打开电源；
[0037] 如果传感器信号满足所设置的参数范围，则继续循环采集传感器信号。
[0038] 本发明具有以下优点：
[0039] 1.节省能耗：本发明与已有的气体驱动在间歇培养供应养分期间靠连续气体驱动来保持培养物与养分的接触方式，耗能高，相比本发明提出的液体驱动是靠水泵，通过电磁阀的开关调节养分与培养物的接触时间，变气体驱动为液体驱动，节省能耗；利用储液罐内安装的消毒措施或在培养液中附加消毒剂进行系统消毒，方便快捷，省却了高压灭菌所带来的高能耗。
[0040] 2.可以规模化生产：本发明通过微环境条件的调控，提高组培物的质量，使外植体达到最佳的生长、分化和代谢状态，整体设计为箱体结构，并且不受时间和地点的限制， 可以随时随地组织规模化生产。
[0041] 3.本发明为提高植物驯化成活率装置，由于微环境可调，因而可以用调节C02和 02浓度的控制方法提供植物生长所需的碳源，取代在培养基中附加糖作为碳源，从而建立正确的碳的库源关系，也为植物向自养方向发展创造了条件。
[0042] 5.用凝胶固体培养基组培诱导生成的根，是气生根，因为凝胶固体培养基中含有非气体相，因而在驯化过程中，原有的根在驯化基质中不起作用，只有重新长出新鲜根才能驯化成活。而用液体间歇培养，诱导生成的根，简短驯化后，就可以栽到温室或大棚地里，简化植株驯化程序，降低成本。而且培养系统保持良好的通风换气系统，因而所培养的植株抗逆性显著增强，提高了组培苗驯化成活率，从而有效降低了生产成本，并且具备现有技术中气升式培养装置的所有优点。
附图说明
[0043] 下面结合附图和实例对本发明进行详细的说明：
[0044] 图1为本发明的整体结构示意图；
[0045] 图2为本发明的调控仓及工作室结构示意图；
[0046] 图3为利用本发明进行培养的结构示意图；
[0047] 图4为本发明控制电路原理图；
[0048] 图5为本发明控制过程流程图（一）；
[0049] 图6为本发明控制过程流程图（二）。
[0050] 附图符号说明如下：1.培养箱，2.工作室，3.营养液存储容器，4.底架，M2.水泵， 6.控制装置，QNC.气体浓度传感器，8.营养液管道，WDC/SDC.温/湿度传感器，10.加湿器， 11.调控仓，12.灯箱，13.风道，14.液位显示计，15.加热/制冷组件，16.出风口，Dl.四通电磁阀，171.第一进气口，172.第一出气口，173.第二出气口，174.第二进气口，18.进气管，19.供气管，20.排气孔，21.进气孔，Ml.风机，22.过滤网，23.液体培养容器，24.逆水阀，25.上液总管路，D3.回液电磁阀，SWC.水位传感器，28.回液总管路。 具体实施方式
[0051] 下面结合附图和实施例对本发明进行详细说明：
[0052] 实施例1
[0053] 如图1、图2、图3所示，本发明装置包括：培养箱1、灯箱12及营养液存储容器3， 其中培养箱1分三层安装于一底架4上部的柜体内，培养箱1为培养植物组织液体的主要装置；灯箱12，内部具有照明灯，设于培养箱1的上部，对培养箱1内部进行照明，可抽拉式灯箱12为植物提供所需的光照；营养液存储容器3，设于培养箱1底部，为培养箱1提供培养植物所需的营养液；可抽拉式灯箱12其内具有的照明日光灯。
[0054] 本实施例采用3个以不同高度置放于底架4上培养箱1，其中每个培养箱1的工作室2中具有3个液体培养容器23，每个液体培养容器23均通过上液总管路25及回液总管路观与营养液存储容器3相连。
[0055] 所述培养箱1包括工作室2、调控仓11及控制装置6，工作室2内部具有液体培养容器23、供气管19，还设有温/湿度传感器WDC/SDC、气体浓度传感器QNC以及水位传感器 SWC，上述各传感器与一控制装置6相连；供气管19通过进气管路18接至调控仓11，液体培养容器23与营养液存储容器3之间设有上液总管路25及回液总管路沈，上水管包括上液总管路25及上/回液支管，上液总管路25上设有水泵M2及逆水阀24，回水管路包括上 /回液支管及回液总管路28，回液总管路观上设有回液电磁阀D3 ；上述各传感器与控制装置6相连。
[0056] 本实施例中工作室2为长方体形状，工作室2内四角处设有四个立柱形的供气管 19，四个供气管19通过通风管18、四通电磁阀Dl与调控仓11内的加湿器10水箱相通；工作室2内设有设有(X)2气体浓度传感器QNC，工作室2还具有C02混合气体的进气孔21及排气孔20 ;C02气体瓶与混合气体瓶连通，并与高压空气在混气瓶内混合经设有电磁阀的气管与工作室2的进气孔21连通，通过气体流量计调节气体流量提供所需浓度的(X)2混合气体，控制培养箱1内混合气体浓度。
[0057] 本实施例中调控仓11内设有加湿器10、风机Ml、风道13、四通电磁阀D1，所述加湿器10的水箱底部设有风道13，风道13中安装有加热/制冷组件15(本实施例采用陶瓷片），风机Ml经过滤网22设置在风道13 —端的进风口处，自然空气在风机Ml的作用下经风道13进行加热或制冷；所述风道13的进风口通过风机Ml连续的引入自然空气，当不需要加湿的时候，出风口 16经过四通电磁阀Dl的第一进气口 171及第一出气口 172由进气管18连接至工作室2的供气管19，改变工作室2内的温度；所述四通电磁阀D1，当需要加湿的时候，空气在风机Ml的作用下经风道13进行加热或制冷，出风口 16经过四通电磁阀 Dl的第一进气口 171其第二出气口 173接回至加湿器10水箱中，加湿器10水箱输出端与四通电磁阀Dl的第二进气口 174相连通，空气加湿后经过四通电磁阀Dl的第二进气口 174 及第一出气口 172由进气管18连接至工作室2的供气管19，改变工作室2内的温湿度；调控仓11内的加湿器10采用超声波加湿器；加湿器10水箱内壁上设有具有消毒作用的光触媒涂层，加湿器10水箱内具有发光电极。光触媒在发光电极的照射产生具有超高氧化能力的氢氧自由基（OH)和氧负离子（O2-)，并与有害气体结合成无害的二氧化碳和水；风机Ml及四通电磁阀Dl与控制装置6连接。
[0058] 所述营养液存储容器3设置于底架4下部，通过上液总管路25及多个上/回水支管分别与多个液体培养容器23连接，在上液总管路25上设有水泵M2及逆水阀M，各个上 /回水支管同时通过回液总管路观接回至营养液存储容器3，该回液总管路观上设有回液电磁阀D3 ；液体培养容器23内设水位传感器SWC，用于检测水位信息，当需要营养液回流时，液体培养容器23通过控制装置6开启控制回液电磁阀D3，经上/回水支管及回液总管路观回流到营养液存储容器3中；本发明装置还可在营养液存储容器3处设有消毒装置， 其消毒装置可为紫外灯、臭氧发生器、光触媒、微过滤器等。
[0059] 控制装置6设于调控仓11的面板上，其电源插头及数据传输接口设于调控仓11 的一侧，控制装置6的控制面板上具有控制按钮SA、SB, Sc, Sd, Se及显示器xp，其中控制装置 6可通过数据传输RS-485接口与远程计算机进行通讯连接；
[0060] 如图4所示，所述控制装置6具有单片机U1，该单片机的通讯管脚A、B、通过数据传输接口与远程计算机进行通讯连接，该数据传输接口可为RS-485接口，RS-232接口， RS-422接口，蓝牙接口或红外接口，本实施例采用RS-485接口 ；单片机Ul的输入端分别接有各控制按钮\、Sb, S。、Sd, &、水位传感器SWC、温度/湿度传感器WDC/SDC及气体浓度传感器QNC ；单片机Ul的输出端分别通过第1开关Sl接至制冷/加热组件，通过第2开关接至灯箱12中的日光灯L，通过第3开关S3分别接至适度控制四通电磁阀D1、温度控制电磁阀D2、水泵M2、回液电磁阀D3、进气电磁阀D4的线圈及风机Ml。
[0061]如图 5
[0062] 本发明控制方法具有以下步骤：
[0063] 开机，控制装置初始化；设定环境变量及控制参数；判断上述控制参数中的控制参数执行时间是否结束；
[0064] 如没有结束，则控制装置采集并显示各传感器检测信号；控制装置将传感器检测信号与控制参数进行比较；
[0065] 如果传感器检测信号没有满足控制参数要求，控制装置输出控制信号启动相应的执行机构进行处理，存储检测信息；如果传感器检测信号满足控制参数要求，存储检测信息；
[0066] 判断控制过程是否结束；如没有结束信息，则返回判断上述控制参数的执行时间是否结束阶段，否则结束控制过程；如果判断上述控制参数的执行时间是否结束的结果为是，则返回至设定控制参数阶段。
[0067] 所述设置控制参数包括具有η套（η为自然数，50，本实施例中η = 10，） 每套控制参数包括培养植物所需的温度及湿度、所需气体浓度、按植物生长所需光照设置光照的时间、按植物生长所需设置营养液的液位高度及液位保持时间以及每套控制参数执行时间。
[0068] 所述控制装置对传感器检测信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程；其中液位控制过程包括以下步骤：判断液体培养容器23内液位是否达到设定高度；进入高水位时间段，如没达到设定高度，则启动水泵Μ2给液体培养容器23添加培养液至设定高度；判断高水位时间段是否结束；如果没有结束，则继续保持高水位；如果有结束，则打开回液电磁阀D3，进入低水位时间段;判断低水位时间是否结束，如果没有结束，则继续保持低水位时间段；如果结束，则进入高水位时间段，实现高低液位的循环供应液体。
[0069] 所述气体浓度控制过程包括以下步骤：判断(X)2气体浓度是否高于设定浓度；如果高于设定浓度，则排出气体；如果低于设定浓度，则吹入(X)2气体。
[0070] 所述温/湿度控制过程包括以下步骤：判断温/湿度是否高于设定温/湿度；如果高于设定温度，则启动制冷组件，制冷吹入的空气；如果低于设定温度，则启动加热组件，加热吹入的空气；如果高于设定湿度，则风机吹入自然的空气；如果低于设定湿度则启动加湿装置，加湿吹入湿的空气。
[0071] 实施例2
[0072] 如图6所示，本发明提供的一种用于植物组织液体培养的装置中进行培养的控制方法，包括以下步骤：
[0073] 按控制按钮，设定环境变量及控制参数，系统巡检时间为30秒，检测一次传感器环境参数，并传递给单片机U1，单片机Ul将当前环境参数与目标控制参数进行对比；控制装置6输出控制信号启动相应的执行机构进行处理，执行机构包括风机Ml、水泵M2、加湿器 10、加热/制冷组件15、照明灯以及各电磁阀；所述控制装置6对传感器信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程。
[0074] 开机，控制装置初始化；设定环境变量及控制参数，调用EEROM内的第η套（η为自然数，1 ^η^ 50，本实施例中η = 10)控制参数循环信息；开始时间暗室；执行第1循环的目标环境参数：预定控制时间为5天；设定温度25V ；湿度40% RH ；CO2气体浓度为 700ppm ；光照时间10h，暗时间14h ；高水位时间lh，低水位时间Mi ；手动设置水位传感器高度为5cm ；
[0075] 保持温度稳定在25士2°C度左右，目的在于因幼苗比较弱，温度差不要过大；如果温度传感器测得环境温度为15°C，低于设定25°C目标值，单片机发出信号启动电子加热片，提高环境温度；如果测得环境温度为26°C，高于目标设定值，单片机发出信号启动制冷片，降低环境温度。提高或降低温度直到设定值25°C ；
[0076] 前期培养要求保持较大湿度，减少干旱胁迫；湿度40% RH ；如果湿度传感器测得环境湿度为30% RH，低于设置40% RH，单片机发出信号启动加湿器同时发光电极发光，提高环境湿度；如果湿度传感器测定环境湿度为45% RH，高于设定值40% RH，单片机发出信号关闭加湿器，利用通风减少空气湿度，提高或降低湿度直到设定值40% RH ；
[0077] CO2气体浓度为700ppm ；当(X)2浓度传感器测到环境中的(X)2浓度为500ppm，低于 700ppm,单片机发出信号开通电磁阀，把(X)2混合气体通入到培养箱1 ；当(X)2浓度为SOOppm 高于预定值时，单片机发出信号关闭电磁阀，利用排风口通风减少其中的CO2浓度，提高或减少其中(X)2浓度直到与预先设定值一致；
[0078] 第一循环参数设定较短的光照，光照时间10h，暗时间14h启动时按照预先设置程序启动，先暗培养，单片机循环检测，如果暗培养3h，低于设置14h，继续暗培养，直到与设定值14h相一致时，单片机发出信号启动光培养，光培养时间为10h，当单片机检测到光培养时间达到预定值IOh时，则循环进入到下一次的暗培养；
[0079] 按植物生长所需设置营养液的液位保持时间和液位高度，前期培养要求较短浸没时间；手动设置水位传感器高度为5cm ；高水位时间lh、低水位时间他；低水位时间是培养容器内尚无营养液时间，高水位时间是利用水泵上水及水位控制时间。低水位时间结束后， 开始上液，在高水位时间内，当营养液上升到设定高度5cm，水位传感器会把水位信息传递给单片机，单片机发出信号通过控制电源，停止水泵工作，并保持一段时间，直到高水位时间结束，单片机发出控制信号打开营养液回液电磁阀26，把营养液回流到营养液存储容器 3内。
[0080] 经比较循环进行时间长度和预定运行时间长度，如果小于等于5天，则继续执行第1循环；如果大于5天，则结束本循环，开始调用EEROM内的第2循环控制参数信息，开始执行第2循环内的控制参数信息，直到第2循环内的进行时间长度满足预定运行时间长度； 则开始调用EEROM内的第3循环控制参数信息；依次循环直到接收到结束信息，培养箱1结束循环工作，培养成功完成，结束整个培养过程。
[0081] 在上述循环培养过程中可根据需求通过控制面板内的选择参数\按钮、移动键， 选择修改位置&按钮、增加键&按钮、降低键、运行键&按钮、复位键&按钮的按钮时时修改当时循环的控制参数，修改后该循环进行时间长度为该循环的初始时间长度，预定运行时间长度不变；温度、湿度、CO2气体浓度、高水位时间，低水位时间；按运行键&开始运行， 执行新的控制信息；
[0082] 总之，根据微环境参数设定，自动调节微环境中温湿度、C02浓度、02浓度和光照时间，完成培养物组织培养液的间歇供应，除了总电源开关外，其它电源开关都由控制装置控制。
[0083] 以往用凝胶固体培养基组培诱导生成的根，是气生根，因为凝胶固体培养基中含有非气体相，因而在驯化过程中，原有的根在驯化基质中不起作用，只有重新长出新鲜根才能驯化成活。而本发明用液体间歇培养，诱导生成的根，可以直接栽到温室或大棚地里，简化植株驯化程序，降低成本。而且培养系统保持良好的通风换气，因而所培养的植株抗逆性显著增强，提高了组培苗驯化成活率，从而有效降低了生产成本，并且具备现有技术中气升式培养装置的所有优点。
[0084] 从上述说明可以看出，本发明的实质是提供一种设计合理，同时又能实现液体培养、微环境可调、半自动化培养的目的；因此，本发明所说的部件不局限于上述具体形状和结构。
Claims (7)
1. 一种用于植物组织液体培养的装置，其特征在于：包括培养箱（1)，为植物组织液体培养的主要装置；灯箱（12)，内部具有照明灯，设于培养箱（1)的上部，对培养箱（1)内部的照明，提供植物所需的光照；营养液存储容器（3)与培养箱（1)连接，为培养箱（1)提供培养植物所需的营养液； 所述培养箱（1)包括工作室O)、调控仓（11)及控制装置（6)，工作室O)内部具有液体培养容器（23)、供气管（19)，还设有温/湿度传感器（WDC/SDC)、水位传感器（SWC)、以及气体浓度传感器（QNC)；供气管（19)通过进气管（18)接至调控仓（11)，液体培养容器03) 与营养液存储容器C3)之间设有上液总管路0¾及回液总管路（¾)，上液总管路05)上设有逆水阀04)和水泵（Μ》，回液总管路08)上设有回液电磁阀（D!3)；上述各传感器与控制装置（6相连；所述调控仓（11)内具有加湿器（10)水箱，该加湿器（10)水箱底部设有风道（13)，风道（1¾外壁上安装有加热/制冷组件（1¾ ；所述风道（1¾的进风口通过风机（Ml)引入空气，出风口（16)经过一电磁阀连接至工作室O)的供气管（19)中；所述电磁阀为四通电磁阀（Dl)，其第一进气口（171)与风道（15)的出风口（16)相连， 第一出气口（17¾通过进气管（18)接至工作室O)的供气管（19)；第二出气口（173)接回至加湿器（10)的水箱中，四通电磁阀（Dl)的第二进气口（174)与加湿器（10)的水箱相连通。
2.按权利要求1所述的一种用于植物组织液体培养的装置，其特征在于：加湿器（10) 水箱内壁上具有光触媒涂层，加湿器（10)水箱内具有发光电极。
3. 一种用于权利要求1所述植物组织液体培养装置的控制方法，其特征在于具有以下步骤：开机，控制装置初始化； 设定环境变量及控制参数；判断上述控制参数中的控制参数执行时间是否结束； 如没有结束，则控制装置采集并显示各传感器检测信号； 控制装置将传感器检测信号与控制参数进行比较；如果传感器检测信号没有满足控制参数要求，控制装置输出控制信号启动相应的执行机构进行处理，存储检测信息；如果传感器检测信号满足控制参数要求，存储检测信息； 判断控制过程是否结束；如没有结束信息，则返回判断上述控制参数的执行时间是否结束阶段，否则结束控制过程。
4.按权利要求3所述植物组织液体培养装置的控制方法，其特征在于：如果判断上述控制参数的执行时间是否结束的结果为是，则返回至设定控制参数阶段。
5.按权利要求3所述植物组织液体培养装置的控制方法，其特征在于：所述设置控制参数包括η套，每套控制参数包括培养植物所需的温度及湿度、所需气体浓度、按植物生长所需光照培养时间、按植物生长所需设置营养液的液位高度及液位保持时间以及每套控制参数执行时间。
6.按权利要求3所述植物组织液体培养装置的控制方法，其特征在于：所述控制装置对传感器检测信号及控制参数进行比较包括液位控制过程、气体浓度控制过程、温/湿度控制过程、光照时间控制过程；其中液位控制过程包括以下步骤：判断液体培养容器内液位是否达到设定高度；如没达到设定高度，则进入高水位时间段，启动水泵给液体培养容器添加培养液至设定高度；判断保持液位高度的保持时间是否达到；如果达到，则打开回水电磁阀，否则继续保持高水位；其中气体浓度控制过程包括以下步骤：判断CO2气体浓度是否高于设定浓度；如果高于设定浓度，则排出气体；如果低于设定浓度，则吹入(X)2气体。
7.按权利要求6所述植物组织液体培养装置的控制方法，其特征在于：所述温/湿度控制过程包括以下步骤：判断温/湿度是否高于设定温/湿度；如果高于设定温度，则启动制冷组件吹入冷空气；如果低于设定温度，则启动加热组件吹入热空气；如果高于设定湿度，则不加湿，通过气体流通降低湿度；如果低于设定湿度则启动加湿装置吹入湿的空气；执行机构包括风机（Ml)、水泵(M2)、加湿器(10)、加热/制冷组件（15)、灯箱(12)以及各电磁阀；光照时间控制过程包括以下步骤：判断是否达到设定的光照时间，如果达到设定的光照时间，则关掉电源；如果未达到设定的光照时间，则打开电源；如果传感器信号满足所设置的参数范围，则继续循环采集传感器信号。
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