CN101297820A - Streptococcus faecium function signal molecule formulation and product thereof for reducing fat and slimming - Google Patents
Streptococcus faecium function signal molecule formulation and product thereof for reducing fat and slimming Download PDFInfo
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Abstract
The invention relates to a streptococcus faecium function signal molecule preparation, a streptococcus faecium bacteria strain is carried out the anaerobic fermentation at 35 to 38 DEG C and the centrifugal concentration to obtain a concentrate of streptococcus faecium bacteria, the bacterial cell wall is broken by high pressure, and the freeze-drying is carried out to obtain the preparation raw powder containing the streptococcus faecium function signal molecules. As the streptococcus faecium function signal molecules can be combined with a specific receptor at the intestinal epithelial cells, the issued signal can induce the excessive cholesterol in the body to carry out the transformation, control the activity of the cholesterol synthase, reduce the excessive cholesterol generated or absorbed in the human body, and effectively control the obesity. The streptococcus faecium function signal molecule preparation is simultaneously matched with a bifidobacterium function signal molecule preparation, a lactobacillus function signal molecule preparation and a bacteroides function signal molecule preparation to be prepared into a series of products containing various probiotics function signal molecules for lipid-lowering and weight-losing, thus fundamentally reducing the risks of coronary heart disease, atherosclerosis, heart diseases, angina, myocardial infarction, obesity and other diseases during the process of being eaten by the people.
Description
Technical field
The present invention relates to derive from the field of the pharmaceutical products of microbial material, particularly derive from the pharmaceutical products of antibacterial.
Background technology
Unite the 4th the Chinese residents nutrition and the health survey data show of issue according to health ministry, the Department of Science and Technology and State Statistics Bureau, national hypertension number of patients and dyslipidemia number have reached 1.6 hundred million; The onset diabetes number has occupied first of the world, reaches 2,380 ten thousand; Overweight and fat number amounts to 2.6 hundred million.Even more serious is, these disease incidence speed rapid growths, and rejuvenation phenomenon [1] has appearred.China 16 years old and above resident's hypertension prevalence have risen 31% over 10 years, and obesity rates has risen 97%.Subsidize and unfolded investigation discovery by Singapore's Ministry of Public Health, have the obesity child of half may suffer from sleep apnea approximately, and in the middle of the child of these obesities, also have 25%～50% may be diabetics.Studies show that obesity can reduce the life-span more than 20 years, get over obesity at an early age, the life-span of minimizing is just many more, every overweight 10～30 pounds of body weight, and the life-span will shorten 3 years [2].The hypertensive sickness rate of moderate Obese children surpasses 30%; The sickness rate of severe Obese children fatty liver also surpasses 30%, and 80% children obesity will extend to adult's stage.Therefore children obesity is laid " basis " for the adult suffers from arteriosclerosis, cardiovascular and cerebrovascular disease, liver cirrhosis, diabetes and hypertension chronic diseases just.Nearly 1/3 colon cancer, breast carcinoma, renal cancer and digestive tract cancer are all relevant with obesity, and the intravital cholesterol level of most of obese patients is higher than average index simultaneously, and the probability of suffering from cardiovascular disease also significantly improves [3-5].Cardiovascular system diseases has become one of principal element of population of China death.Therefore reduce the intravital cholesterol of people, eliminating obesity is the only way which must be passed that the mankind keep fit.
Cholesterol is a kind of lipid, it is indispensable material in each human body, cholesterol is the necessary nutritional labeling of human survival, especially in cell membrane component and hormone synthetic, plays an important role, and also be the raw material of synthesis of vitamin d 3 and the precursor of bile acid [6-8].If but body inner cholesterol too high levels is suffered from the just raising greatly of probability of diseases such as coronary heart disease, atherosclerosis, heart disease, angina pectoris, myocardial infarction and obesity, the danger that suffers stroke also increases [9] thereupon.Cause the high reason of cholesterol to have a lot, the one, because eat too much Animal fat, saccharide and protein etc., the cholesterol that absorption of human body is too much, cholesterol level also increases in the corresponding blood.The 2nd, the patient who takes broad ectrum antibiotic for a long time can make and produce Resistant strain in the body, and body cell immunity, humoral immune function descend, especially make little ecological disturbance in the intestinal, the enteral probiotics is suppressed, the ability drop of reduction cholesterol, cause the increase [10,11] of body inner cholesterol content.Over nearly 30 years, because animal husbandry is added on utilization rate and the quickening growth of animal to increase feedstuff in the feedstuff with antibacterials as growth promoter, the intravital enterococcus of animal has produced various drug resistance under the selection pressure effect of antibacterials, even the multiple drug drug resistance appearred, and the life-time service antibiotic is residual in animal products meat, egg, milk, directly threatens health.Therefore, regulate microecological balance in human body and the animal body, could fundamentally remove the mankind and be perplexed this huge difficult problem [12-15] by chronic disease.
Streptococcus faecalis (S.faecium) is exactly the probiotic bacteria that preferably comes out the normal microorganism in human body, and through test in vitro, animal experiment and human trial, verified this probiotic bacteria is nontoxic, harmless, safe, effective.U.S. food in 1989 and Drug Administration (FDA) announce that streptococcus faecalis is for can be directly used in one of strain of animal.Streptococcus faecalis can secrete a kind of material that is made of polysaccharide, protein and ribonucleic acid in growth course, these materials can promote the cholesterol decomposition metabolism, makes cholesterol be transformed into bile acid, scalable human body blood lipid level, control cholesterolemia content [16].Research think streptococcus faecalis virulence a little less than, it is the intravital normal flora of people, seldom cause human infection, its viable bacteria body is sure as a kind of curative effect of probiotics micro-ecological regulator, has been widely used in human health care and prophylactic effective ingredient in worldwide.But this goods have strict requirement, at first enough viable counts will be arranged, its less important survival period that certain hour is arranged.This has proposed strict requirement to production and selling, thereby also limits the development of these class health product.In actual applications, production circle is in order to overcome above-mentioned two restrictions, proposed again with dead thalline as this based article effective ingredient, thereby avoided the bottle neck effect of production and selling.Though second filial generation product has been avoided viable count and two bottleneck restrictions of survival period, and fatal shortcoming is also arranged, Here it is, and dead thalline is difficult to quantize, and is unfavorable for quality control, in fact is difficult to become qualified product.Except the restriction of viable count and survival period, also have the restriction of traditional sanitary standard, route of administration is single can only to be oral.Drawbacks limit such as viable count and dead bacterium self-dissolving the exploitation of many probiotic bacteria new products, also limited the reinforcement of probiotic bacteria function simultaneously.
We develop the little ecological product of the third generation, i.e. probiotic bacteria function signal molecular preparation again on the basis of pro-little ecological product shortcoming of two generations.The sort signal molecule is the important component part in contemporary intercellular communication (the cell to cell cammunication) system.The effect main body is to provide health signal to host cell, makes host cell produce corresponding physiological action and healthy performance.The mechanism of action of streptococcus faecium function signal molecule formulation is exactly that its function signal molecule combines with specific receptor at enterocyte, impels host cell to produce corresponding healthy physiological reaction [17,18].Streptococcus faecium function signal molecule can make the cholesterol allosteric of getting rid of through biliary tract in the liver sausage circulation, make the interior unnecessary cholesterol of body be converted into the non-absorbent coprostenol of human body, can not be absorbed by liver through vein again, block the liver sausage circulation of cholesterol, can effectively control cholesterol level in the blood plasma.Streptococcus faecium function signal molecule also can suppress the vigor that cholesterol biosynthesis rate-limiting enzyme is a 3-hydroxy-3-methyl glutaryl CoA enzyme, thereby suppresses the aggregate velocity of body inner cholesterol.Can also control simultaneously reuptaking of cholic acid in the intestinal, the cholic acid content in the liver is descended, impel unnecessary cholesterol in liver, to be converted into cholic acid, thereby reduce the content of serum cholesterol.Streptococcus faecium function signal molecule sends signal and makes enteral cholate hydrolysis, cholate after the hydrolysis can with the cholesterol generation co-precipitation in the food, thereby promote the cholesterol in the food source in feces, to shift, and under the synergism of bifidobacterium function signaling molecules, in time excrete.Streptococcus faecium function signal molecule is for preventing that cardiovascular disease such as human body artery is atherosis from having played positive effect [19].Prove that through stability and various toxicity tests streptococcus faecium function signal molecule formulation has no side effect, and have the stability of heredity.Studies have shown that in a large number that in recent years this is the huge biological preparation of New-generation healthy effect, its medical treatment, health care also will further enlarge.In addition, use the thalline important component, help the application and the popularization of qualitative, quantitative quality control and product as detecting index.
At present, the report of probiotic bacteria function signal molecule (PSM) preparation is less, and has no precedent report with probiotic bacteria function signal molecule (PSM) as the lowering blood-fat and reducing weight product.
List of references
[1] the DM891129 bacterial strain is to the influence [J] of sarsaparilla Mus hypercholesterolemia. Chinese microecology magazine, 1991,3 (4): 67～69
[2] enterococcus is to the preliminary study [J] of ecological agent to the human serum cholesterol levels. Chinese microecology magazine, 1992,4 (3): 16～19
[3] enterococcus and relevant microecologic regulator [J]. Chinese microecology magazine, 1998,10 (1): 59～61
[4] the happy Preliminary Clinical Observation result of the fat pier of ecological goods [J]. Chinese microecology magazine, 1992,4 (4): 136
[5] health and fitness information opinion [M]. publishing house of the Maritime Affairs University Of Dalian, 2006
[6] Senbaiao Science ﹠ Technology Co., Ltd., Dalian. be used to reduce the probiotics preparation and the technology of people or livestock blood clearing gallbladder sterin. application number: 200610045955[P], 2006
[7] research [J] of enterococcus DM981129 bacterial strain cholesterol reducing effect in vitro. Chinese microecology magazine, 1993,5 (1): 8～10
[8] enterococcus and relevant microecologic regulator [J]. Chinese microecology magazine, 1998,10 (1): 59～60
[9] preliminary study [J] of enterococcal separation in pig source and biological nature. Chinese microecology magazine, 2001,13 (3): 135～137
[10] enterococcus faecalis DM981129 bacterial strain is to the regulating action [J] of rat hyperlipidemia. Chinese microecology magazine, 1999,11 (6): 377～378
[11] research of enterococcus faecalis active bacteria formulation [J]. Chinese microecology magazine, 2003,15 (1): 20～21
[12] clone of Enterocin A structural gene, functional expression and structure activity study. the doctorate paper. Jilin: Jilin University, 2005
[13] the not quantitative analysis [J] of the main flora of consubstantiality fat crowd intestinal. health research, 2005,34 (6): 724～725
[14] enterococcal drug resistance and drug resistant gene detect [J]. modern practical medical science, 2004,16 (7): 385～386
[15] screening of efficient lactobacillus for degrading cholesterol and the application in fermentation milk thereof. master thesis. the Inner Mongol: Agricultural University of the Inner Mongol, 2006
[16] screening of lactobacillus for degrading cholesterol and biological characteristic research thereof. master thesis. Nanjing: Nanjing Normal University, 2005
[17] the external cholesterol-lowering activity research of human body intestinal canal probiotic bacteria [J]. microorganism journal, 2005,45 (6): 920～924
[18] enterococcal separation of human body cholesterol reducing and evaluation [J]. Shaoxing texture institute journal, 2006,26 (10): 44～47
[19] screening of probiotic bacteria and Study on Functional Properties. master thesis. Harbin: Northeast Agricultural University, 2002
Summary of the invention
Deficiency according to the above present probiotic products, in conjunction with " health and fitness information opinion " theory, the objective of the invention is to effectively extract the function signal molecule in the streptococcus faecalis, make streptococcus faecium function signal molecule formulation, cooperate bifidobacterium function signaling molecules preparation, bacillus acidophilus's function signal molecular preparation and bacteroid function signal molecular preparation simultaneously and then make the lowering blood-fat and reducing weight series of products that contain multiple probiotic bacteria function signal molecule (PSM).
The preparation technology of the former powder of streptococcus faecium function signal molecule formulation of the present invention comprises:
1, streptococcus faecalis its thalline that ferments concentrates: the weight ratio that contains that the streptococcus faecalis seed liquor is inserted after having sterilized is: soy peptone 0.5%～1.0%, show in the culture medium matrix of peptone 0.5%～1.0%, glucose 0.5%, serum powder 0.5%～1.0%, potassium dihydrogen phosphate 0.25%, Carnis Bovis seu Bubali cream 0.25%, Hepar Bovis seu Bubali powder 0.15%, yeast extract 0.5%～1.0%, sodium chloride 0.3%, soluble starch 0.5%, sodium thioglycollate 1.5%, behind 35～38 ℃ of anaerobic fermentations, promptly obtain streptococcus faecalis thalline concentrate through centrifugal concentrating again.
2, cell wall breaking:, use high pressure cell disintegrating machine operating pressure 100 ± 10MPa with the broken somatic cells wall of high pressure with this streptococcus faecalis thalline concentrate adding distil water or normal saline (1: 3～1: 5).
3, drying: till the solvent-free flavor, or the trehalose that adds 100～200ml carries out lyophilization as freeze drying protectant with these goods to cell extract in 40～70 ℃ of drying under reduced pressure.The dry thing that obtains promptly gets the former powder of streptococcus faecium function signal molecule formulation through grinding, sieving.Its preparation raw powder is white to pale powder, can dissolve fast in water, and is stable down with refrigerated condition at normal temperatures.
The test item of streptococcus faecium function signal molecule formulation of the present invention and index feature are:
(1) cell wall sugar inspection takes by weighing former powder 0.01g and adds 15% n-butanol aqueous solution and respectively be mixed with 10mg/ml solution.0.5ml is in test tube in absorption, drips 0.05% anthrone sulphate reagent 1.0ml, shakes up promptly to be aeruginous.
(2) cell wall meso meso diaminopimelic acid is checked.Adopt ply of paper to analyse-development process, should show distinctive olive-green speckle, back flavescence color fades.
(3) outward appearance should be white powder.
(4) loss on drying inspection, subtracting weight loss should not be higher than 5%.
The preparing process that contains probiotic bacteria function signal molecule (PSM) lowering blood-fat and reducing weight series of products of the present invention is as follows:
Probiotic bacteria function signal molecule (PSM) preparation raw powder 0.1%～5.0%
Batching 95.0%～99.9%
Wherein the proportioning in the batching is:
Prebiotics 0.0%～46.0%
Sweeting agent 0.0%～65.0%
Adjuvant 30.0%～100.0%
Be processed into different dosage forms such as oral liquid, injection, powder, capsule, tablet or granule as required.
Wherein said probiotic bacteria function signal molecular preparation comprises streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation, bacillus acidophilus's function signal molecular preparation, bacteroid function signal molecular preparation.
Described batching comprises prebiotics, sweeting agent and adjuvant:
Described prebiotics comprises a kind of independent use or more than one mixing uses in oligofructose, oligomeric galactose, soybean oligo saccharide, oligomeric xylose, oligomeric lactulose, oligomeric dragon gallbladder sugar, oligomeric isomaltose, palatinose, cyclodextrin, newborn sugarcane powder, stachyose, cottonseed sugar, manna oligosaccharide, trehalose, inulin or the inulin oligofructose etc.As composition of food, some non-digestible carbohydrates such as patience starch, plant cell wall polysaccharides, hemicellulose, pectin and natural gum etc.; Non-digestibility oligosaccharide, some polypeptide or protein and some lipid also can be used as the candidate of prebiotics.
Sweeting agent comprises sugar sweetener and non-sugar sweetener, sugar sweetener comprises all known saccharides at present, for example sucrose, glucose, maltose, dextrin, dry powder Nulomoline, fructose, laevulose, galactose, primverose starch or with its molasses of making, syrup or the like can a kind of independent use also can more than one mix and use.Non-sugar sweetener (have sweet taste but do not have the characteristic of known sugars) comprises Sorbitol, mannitol, xylitol, hydrogenated starch hydrolysate, maltose alcohol, stevioside, aspartame, Flos Chrysanthemi essence, cyclamate and aspartame etc., can a kind of independent use also can more than one mix and use.Sugar sweetener and non-sugar sweetener also can mix use simultaneously.
Adjuvant comprises the filler that can make oral liquid, as Chinese medicine extraction liquid or Chinese medicine powders such as Radix Ginseng, Radix Angelicae Sinensis, Flos Caryophylli, Radix Polygoni Multiflori, Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi, Semen Cassiae, Ganoderma, Radix Gentianae, Radix Salviae Miltiorrhizae, Radix Glycyrrhizae, Herba Menthae, the Radix Astragali and Semen Nelumbinis; Also can comprise fruice such as Fructus Fragariae Ananssae, Fructus Mali pumilae, Fructus actinidiae chinensis, Fructus Musae, Fructus Citri junoris, Fructus Vitis viniferae, Fructus Citri grandis, Fructus Ananadis comosi and fruit, dry fruit extracting solution; Can also comprise natural food fibres, dry powder, tea powder, aminoacid, soybean lecithin, vitamin, Mel, pollen, trace element, flavorant, citric acid, Semen Maydis powder, Testa Tritici powder, soy milk powder, milk powder, coffee powder, egg albumen powder, oatmeal, black sesame powder, drinking water etc.
Can add probiotic bacteria function signal molecular preparation in the products such as mineral water, milk product, soda pop, tea beverage, juice-type beverage, canned food, oral hygiene goods, instant food, instant food, cookies, puffed food, instant food, cold drink food, cake, drinks and flavoring agent to simultaneously, make the health food that contains profitable probliotics function signal molecule (PSM).
Probiotic bacteria function signal molecule (PSM) not only can be added in the human food, simultaneously also can be used as feed additive adds in the animal feed, replace traditional antibacterials as animal growth promoting agent, the utilization rate that increased feedstuff has also been avoided in animal body residual of antibiotic medicine.
Compared with prior art, the invention is characterized in:
(1) streptococcus faecium function signal molecule formulation can make the cholesterol allosteric of getting rid of through biliary tract in the liver sausage circulation, make the interior unnecessary cholesterol of body be converted into the non-absorbent coprostenol of human body, can not be absorbed by liver through vein again, block the liver sausage circulation of cholesterol, can effectively control cholesterol level in the blood plasma.
(2) streptococcus faecium function signal molecule formulation also can suppress the vigor that cholesterol biosynthesis rate-limiting enzyme is a 3-hydroxy-3-methyl glutaryl CoA enzyme, thereby suppresses the aggregate velocity of body inner cholesterol.
(3) streptococcus faecium function signal molecule formulation can also suppress reuptaking of cholic acid in the intestinal, and the cholic acid content in the liver is descended, and impels more cholesterol to be converted into cholic acid in liver, thus the content of control serum cholesterol.
(4) streptococcus faecium function signal molecule sends signal and makes enteral cholate hydrolysis, the cholate after the hydrolysis can with the cholesterol generation co-precipitation in the food, thereby promote the cholesterol in the food source in feces, to shift.
(5) streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation, bacillus acidophilus's function signal molecular preparation, bacteroid function signal molecular preparation stick on human or animal's different intestinal segments respectively, the mechanism of action of its cholesterol reducing is also different, get up to make them to play a role jointly with different mechanisms four kinds of probiotic bacteria function signal molecular combinations, have complementary advantages at intestinal segment separately.
In addition, streptococcus faecium function signal molecule formulation of the present invention and extraction process thereof, and the lowering blood-fat and reducing weight series of products that contain multiple probiotic bacteria function signal molecule have following 5 advantages:
(1) can increase the effect duration and the shelf life of product without the viable bacteria body;
(2) not only can orally also can increase route of administration, as respiratory tract spraying, dermal osmosis and injection (subcutaneous, muscle or vein) etc.;
(3) pharmacokinetics detects easily and realizes;
(4) long shelf-life;
(5) the little convenient transportation of volume.
The specific embodiment
Embodiment one:
It is the secondary Wistar male rat of 150～200g that laboratory animal adopts body weight, raise containing 1% cholesterol, and the high lipid food of 5% Adeps Sus domestica and 0.2% cholate, being divided into is 4 groups.Experimental group gives streptococcus faecium function signal molecule formulation of the present invention, is divided into high, medium and low dosage group, and every day is coloclysis 2ml respectively, observes and respectively organizes the rat body weight situation of change, and experimental result is listed in table 1.The result shows that the experimental group animal is compared the body weight difference of respectively organizing rat with matched group do not have significance (P＞0.05).
Respectively organize before and after table 1 experiment rat body weight change (g, M ± SD, n=10)
Annotate: three experimental grouies award and are tried thing enterococcus faecalis function signal molecular preparation, dosage is respectively: low dose group 225mg/kg.BW, in dosage group 450mg/kg.BW, high dose group 900mg/kg.BW is equivalent to 5,10,20 times of human body recommended intake respectively.
Embodiment two:
Rat blood serum concentration before and after the test experiments adopts elisa kit to measure the serum total cholesterol (TC) of rat, and test instrunment is: automatic biochemical analyzer.Experimental result is listed in table 2.Show that from test result when the 14th day and the 28th day, high, medium and low dosage Serum TC concentration increment significantly is lower than matched group (P＜0.05).
Respectively organize before and after table 2 experiment Serum TC concentration (mg/dl, M ± SD, n=10)
Annotate: * compares P＜0.05 with matched group
Embodiment three:
HDL-C helps preventing atherosclerosis, can think a kind of protection factor of cardiovascular system.The ratio of HDL-C/TC is meant the ratio that HDL-C occupies in TC.The ratio of rat blood serum HDL-C/TC is respectively organized in test, by phosphotungstic acid-magnesium (PTA-Mg2+) sedimentation method, adopts elisa kit to measure, and test instrunment is: automatic biochemical analyzer, the result is as shown in table 3.Experimental result shows, all there were significant differences (P＜0.05) with matched group when the 14th day and the 28th day for high, medium and low dosage group.
Table 3 is respectively organized rat blood serum HDL-C/TC ratio (%, M ± SD)
Annotate: * compares P＜0.05 with matched group
Embodiment four:
Atherogenic index (AI) is the index that the reaction cardiovascular disease is easily suffered from degree, by
Calculate, this index is high more, and the easy trouble degree of cardiovascular disease is high more, and the measurement result of rat artery hardenability value is listed in table 4.High, medium and low as can be seen from Table 4 dosage group all is lower than matched group (P＜0.05) when the 14th day and the 28th day.
Table 4 is respectively organized rat artery hardenability value (AI, M ± SD)
Annotate: * compares P＜0.05 with matched group
Therefore, according to the criterion of " the health food function assessment assessment process and the method for inspection ", can think that streptococcus faecium function signal molecule formulation has the effect of regulating rat fat.
Embodiment five:
Streptococcus faecium function signal molecule formulation of the present invention has the effect that reduces serum cholesterol level through external and zoopery proof, and the present invention has same functions of lowering blood-fat and reducing weight by the human experimentation proof.As follows to human test's serum cholesterol, triglyceride levels step: normal control group and the 1st group were taken medicine for 4 weeks, 4 weeks of drug withdrawal, continue 4 weeks of taking medicine again, observed for 12 weeks altogether, per stage is looked into once every index before and after taking medicine, mainly be clinical ordinary circumstance and side effect etc., comprise: general sensory, appetite, every day defecation number of times, sleep, heating situation, routine blood test, urine examination, kidney merit, cholesterolemia, serum triglycerides and blood sugar level, experimental result is listed in table 5.
Table 5 human trial serum cholesterol, triglyceride levels (mmol/L)
Experimental result shows that all pill takers there is no any untoward reaction at period in a medicine and in 2 weeks of back of taking medicine, and general clinical condition is all normal.As can be seen from Table 5, the 8th when week blood TC level slightly rise, this finished for the phase I, the result before second stage is taken medicine, but its numerical value slightly descends again after second stage is taken medicine still within normal range, preceding level is close with taking medicine.As seen streptococcus faecium function signal molecule formulation of the present invention does not have obvious influence to normal blood TC level and TG level, and each stage corresponding index is nonsignificance (P＞0.05) relatively.Simultaneously proving also that through pharmaceutical research streptococcus faecium function signal molecule formulation of the present invention is nontoxic, harmless, safe, reliable, is a kind of preparation that can regulate the effect of human body cholesterol metabolism.
Embodiment six:
The former powder 0.1% of streptococcus faecium function signal molecule formulation (mass percent, down together), oligofructose 21.0%, soybean oligo saccharide 3.9%, trehalose 15.0%, sucrose 26.8%, vitamin powder 3.2%, egg albumen powder 30.0% will be made powder behind the raw material mix homogeneously, is packaged into bag or is distributed into box.
Embodiment seven:
The former powder 0.5% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation 0.5%, the former powder 0.8% of bacteroid function signal molecular preparation, inulin 32.2%, sucrose 19.0%, egg albumen powder 47.0% with making powder behind the raw material mix homogeneously or being pressed into lamellar or graininess, is distributed into box or is distributed into bag.
Embodiment eight:
The former powder 1.5% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 2.0%, the former powder 1.5% of bacillus acidophilus's function signal molecular preparation, oligomeric xylose 7.0%, laevulose 32.0%, aspartame 5.0%, defatted milk powder 51.0%, to make lamellar or graininess behind the raw material mix homogeneously, be packaged into bag or be packaged into box.
Embodiment nine:
The former powder 1.4% of streptococcus faecium function signal molecule formulation, the former powder 0.2% of bacillus acidophilus's function signal molecular preparation, the former powder 1.2% of bacteroid function signal molecular preparation, oligomeric dragon gallbladder sugar 18.2%, maltose 10.0%, mannitol 33.0%, vitamin 3.0%, soybean lecithin 8.0%, coffee powder 25.0%, to make graininess or lamellar behind the raw material mix homogeneously, be packaged into bag or be packaged into box.
Embodiment ten:
The former powder 2.1% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 1.2%, the former powder 1.3% of bacillus acidophilus's function signal molecular preparation, the former powder 0.2% of bacteroid function signal molecular preparation, palatinose 6.0%, inulin 12.0%, Oligomeric maltose 15.0%, Flos Chrysanthemi essence 3.2%, pollen 4.9%, dry powder 53.8%, trace element 0.3%, to make powder behind the raw material mix homogeneously, be packaged into bag, become box or be distributed into jar.
Embodiment 11:
The former powder 0.4% of streptococcus faecium function signal molecule formulation, the former powder 0.1% of bacillus acidophilus's function signal molecular preparation, oligofructose 8.0%, manna oligosaccharide 4.5%, Sorbitol 57.0%, coffee powder 30.0% is with the abundant mix homogeneously of raw material, make the healthy coffee infusion that contains profitable probliotics function signal molecule, be packaged into bag or be packaged into box.
Embodiment 12:
The former powder 7.4g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 2.7g, the former powder 0.9g of bacillus acidophilus's function signal molecular preparation, stachyose 5kg, galactose 2kg, natural food fibres 4kg, make probiotic bacteria function signal molecule lowering blood-fat and reducing weight preparation behind the mix homogeneously, No. 1, packing or No. 0 capsule are used for hyperlipidemia or obese people, 4～8 of adult's doses every day.
Embodiment 13:
The former powder 42.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 21.0g, the former powder 7.0g of bacillus acidophilus's function signal molecular preparation, the former powder 28.0g of bacteroid function signal molecular preparation, soybean oligo saccharide 1kg, Oligomeric maltose 1kg, oligofructose 3kg, aspartame 4.5kg, Radix Glycyrrhizae powder 2kg, Fructus Schisandrae Chinensis powder 2.5kg, fully make probiotic bacteria function signal molecule lowering blood-fat and reducing weight preparation behind the mix homogeneously, No. 1, packing or No. 0 capsule are used for hyperlipidemia or obese people, 3～6 of adult's doses every day.
Embodiment 14:
The former powder 243.0g of streptococcus faecium function signal molecule formulation, the former powder 177.0g of bacillus acidophilus's function signal molecular preparation, oligomeric lactulose 12kg, fructose 10kg, oatmeal 13kg fully makes probiotic bacteria function signal molecule lowering blood-fat and reducing weight preparation, No. 1, packing or No. 0 capsule behind the mix homogeneously, be used for hyperlipidemia or obese people, 4～8 of adult's doses every day.
Embodiment 15:
The former powder 196.0g of streptococcus faecium function signal molecule formulation, cottonseed sugar 2kg, cyclodextrin 0.8kg, glucose 1.2kg, Fruit powder 3kg fully makes behind the mix homogeneously and contains profitable probliotics function signal molecule lowering blood-fat and reducing weight preparation, be squeezed into lamellar or graininess, be distributed into box, be used for hyperlipidemia or obese people, 3～6 of per day for adults doses.
Embodiment 16:
The former powder 241.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 285.0g, the former powder 63.0g of bacillus acidophilus's function signal molecular preparation, the former powder 116.0g of bacteroid function signal molecular preparation, inulin 0.5kg, maltose 9.5kg, tea powder 5.0kg, fully make probiotic bacteria function signal molecule lowering blood-fat and reducing weight preparation behind the mix homogeneously, be squeezed into lamellar or graininess, be distributed into bottle or be distributed into box, be used for hyperlipidemia or obese people, 2～4 of per day for adults doses.
Embodiment 17:
The former powder 1.4% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 2.2%, the former powder 0.6% of bacteroid function signal molecular preparation, oligofructose 40.0%, aspartame 3.0%, cyclamate 2.0%, the Radix Ginseng powder 3.0%, medlar powder 5.0%, drinking water is diluted to 100%, all fusedly be distributed into bottle after evenly, make and contain profitable probliotics function signal molecule oral liquid, be applicable to hyperlipidemia or obese people.
Embodiment 18:
The former powder 1.3% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 2.4%, xylitol 22.0%, the Radix Ginseng extractive solution 12.0%, Mel 7.0%, Sucus Vitis viniferae 34.0% is added into 100% with drinking water, be distributed into bottle and make and contain profitable probliotics function signal molecule oral liquid, be applicable to hyperlipidemia or obese people.
Embodiment 19:
The former powder 0.6% of streptococcus faecium function signal molecule formulation, the former powder 1.3% of bacillus bifidus bacillus function signal molecular preparation, the former powder 2.1% of bacillus acidophilus's function signal molecular preparation, glucose 33.0%, cyclamate 2.4%, Fructus Lycii extracting solution 22.0%, Radix Polygoni Multiflori extracting solution 4.0%, Herba Menthae extracting solution 5.2% is added into 100% with drinking water, be distributed into bottle and make and contain profitable probliotics function signal molecule oral liquid, be applicable to hyperlipidemia or obese people.
Embodiment 20:
The former powder 0.5% of streptococcus faecium function signal molecule formulation, the former powder 2.6% of bacillus acidophilus's function signal molecular preparation, soybean oligo saccharide 7.0%, corn syrup 33.0%, hawthorn extract 13.0%, Semen Cassiae extracting solution 7.0%, juice of Fructus Jujubae 24.0%, be diluted to 100% with drinking water, be distributed into bottle and make and contain profitable probliotics function signal molecule oral liquid, be applicable to hyperlipidemia or obese people.
Embodiment 21:
The former powder 1.5% of streptococcus faecium function signal molecule formulation, the former powder 2.4% of bacteroid function signal molecular preparation, green tea powder 30.0%, Ganoderma powder 5.0%, black sesame powder 13.5%, be diluted to 100% with drinking water, be distributed into bottle and make and contain profitable probliotics function signal molecule oral liquid, be applicable to hyperlipidemia or obese people.
Embodiment 22:
The former powder 2.3% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 0.1%, the former powder 0.3% of bacillus acidophilus's function signal molecular preparation, the former powder 1.6% of bacteroid function signal molecular preparation, inulin 39.7%, maltose 17.0%, aminoacid powder 3.0%, vitamin powder 1.4%, egg albumen powder 34.6%, fully make powdery behind the mix homogeneously, be distributed into bag or be distributed into box.
Embodiment 23:
The former powder 1.6% of streptococcus faecium function signal molecule formulation, the former powder 0.2% of bacillus acidophilus's function signal molecular preparation, oligofructose 19.2%, oligomeric xylose 7.0%, soybean oligo saccharide 5.0%, edible cellulose 36.0%, vitamin powder 2.0%, black sesame powder 16.0%, walnut powder 13.0%, fully make powdery, lamellar or graininess behind the mix homogeneously, be distributed into bag or be distributed into box.
Embodiment 24:
The former powder 1.3% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 2.5%, the former powder 0.2% of bacteroid function signal molecular preparation, oligomeric galactose 6.8%, dextrin 3.2%, maltose 16.0%, soy milk powder 49.6%, amino acid/11 5.2%, vitamin 5.2%, fully make powdery behind the mix homogeneously, be distributed into box or be distributed into bag.
Embodiment 25:
Streptococcus faecium function signal molecule 1.9%, sucrose 26.0%, edible cellulose 32.0%, walnut powder 18.0%, black sesame powder 8.0%, egg albumen powder 8.1%, red date powder 2.5%, soybean lecithin 3.2%, trace element 0.3% is fully made powdery behind the mix homogeneously, is distributed into box or is distributed into bag.
Embodiment 26:
The former powder 0.4% of streptococcus faecium function signal molecule formulation, the former powder 3.6% of bacillus acidophilus's function signal molecular preparation, the former powder 1.0% of bacteroid function signal molecular preparation, oligomeric xylose 21.0%, mannitol 5.0%, corn syrup 6.0%, red date powder 8.0%, Mel 32.0%, black sesame powder 5.6%, the Radix Ginseng powder 2.2%, oatmeal 15.2%, fully be squeezed into graininess or lamellar behind the mix homogeneously, be distributed into bag again or be distributed into box.
Embodiment 27:
Streptococcus faecium function signal molecule formulation 0.2%, bifidobacterium function signaling molecules preparation 1.4%, bacillus acidophilus's function signal molecular preparation 1.3%, oligofructose 11.0%, soybean oligo saccharide 23.0%, milk powder 63.1%, fully behind the mix homogeneously, make the health milk powder that contains profitable probliotics function signal molecular preparation, be packaged into bag or be distributed into box, also can push in flakes, make the milk slice that contains profitable probliotics function signal molecular preparation.
Embodiment 28:
The former powder 370.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 130.0g, glucose 2kg, milk 8Kg, behind the mix homogeneously high temperature sterilize, make the health milk that contains profitable probliotics function signal molecule, be distributed into bag or be distributed into box.
Embodiment 29:
The former powder 0.7% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation 0.2%, the former powder 2.1% of bacteroid function signal molecular preparation, oligomeric lactulose 9.1%, xylitol 2.3%, sucrose 13.0%, oatmeal 72.6%, fully make the healthy oatmeal that contains profitable probliotics function signal molecule behind the mix homogeneously, be distributed into bag or be distributed into box.
Embodiment 30:
The former powder 3.2% of streptococcus faecium function signal molecule formulation, oligofructose 17.0%, maltose 19.0%, Fructus Fragariae Ananssae juice 60.8% is distributed into jar or is distributed into bottle.
The embodiment hentriaconta-:
The former powder 1.2% of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 1.4%, xylitol 52.4%, Radix Angelicae Sinensis powder 3.1%, Radix Polygoni Multiflori powder 4.6%, Semen Nelumbinis powder 2.9%, Fructrs Jujubae Powder 33.0%, Ganoderma powder 1.4% is fully made powder, tablet, granule or is distributed into capsule behind the mix homogeneously.
Embodiment 32:
The former powder 87.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 23.0g, the former powder 10.0g of bacillus acidophilus's function signal molecular preparation, Semen Maydis powder 10kg, fully make powder behind the mix homogeneously, mixed by 1: 100 with feedstuff as feed additive, can be used for the raising of fowl poultrys such as low-cholesterol chicken or pig.
Embodiment 33:
The former powder 29.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 7.0g, Testa Tritici powder 18kg fully makes powder behind the mix homogeneously, mixed by 1: 100 with feedstuff as feed additive, can be used for the raising of fowl poultrys such as low-cholesterol chicken or pig.
Embodiment 34:
The former powder 34.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 569.0g, the former powder 257.0g of bacillus acidophilus's function signal molecular preparation, the former powder 220.0g of bacteroid function signal molecular preparation, Testa Tritici powder 23kg, Semen Maydis powder 13kg fully makes powder behind the mix homogeneously, mixed by 1: 100 with feedstuff as feed additive, can be used for the raising of fowl poultrys such as low-cholesterol chicken or pig.
Embodiment 35:
The former powder 128.0g of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder 59.0g, the former powder 107.0g of bacteroid function signal molecular preparation, oligomeric galactose 5.6kg, Semen Maydis powder 8.4kg fully behind the mix homogeneously, mixed by 1: 1000 with drinking water as feed additive, directly drink, can be used for the raising of fowl poultrys such as low-cholesterol chicken or pig for the fowl poultry.
Embodiment 36:
The former powder 77.0g of streptococcus faecium function signal molecule formulation, the former powder 32.0g of bacteroid function signal molecular preparation, the former powder 121.0g of bacillus acidophilus's function signal molecular preparation, glucose 23.0kg, Semen Maydis powder 23.0kg fully behind the mix homogeneously, mixed by 1: 1000 with drinking water as feed additive, directly drink, can be used for the raising of fowl poultrys such as low-cholesterol chicken or pig for the fowl poultry.
Claims (7)
1. streptococcus faecium function signal molecule formulation, it is characterized in that be by the streptococcus faecalis strain through anaerobic fermentation, cell wall breaking, enzymolysis, slightly carry, dry and pulverize the preparation raw powder that forms,, can in water, dissolve fast to pale powder in vain, stable down with refrigerated condition at normal temperatures; Its index feature is:
(1) cell wall sugar inspection takes by weighing former powder 0.01g and adds 15% n-butanol aqueous solution and respectively be mixed with 10mg/ml solution.0.5ml is in test tube in absorption, drips 0.05% anthrone sulphate reagent 1.0ml, shakes up promptly to be aeruginous;
(2) cell wall meso meso diaminopimelic acid is checked.Adopt ply of paper to analyse-development process, should show distinctive olive-green speckle, back flavescence color fades;
(3) outward appearance should be white powder;
(4) loss on drying inspection, subtracting weight loss should not be higher than 5%;
2. according to the described streptococcus faecium function signal molecule formulation of claim 1, it is characterized in that containing the lowering blood-fat and reducing weight goods based on the former powder of probiotic bacteria function signal molecular preparation of streptococcus faecium function signal molecule, its contained component and quality per distribution ratio are:
The former powder 0.1～5.0 of probiotic bacteria function signal molecular preparation
Batching 95.0～99.9
Wherein: the former powder of described probiotic bacteria function signal molecular preparation is 1～4 kind in the former powder of streptococcus faecium function signal molecule formulation, bifidobacterium function signaling molecules preparation raw powder, the former powder of bacteroid function signal molecular preparation, the former powder of bacillus acidophilus's function signal molecular preparation;
Described batching comprises prebiotics, sweeting agent and adjuvant; Its mass percent is:
Prebiotics 0.0～46.0
Sweeting agent 0.0～65.0
Adjuvant 30.0～100.0
Wherein: described prebiotics comprises one or more in oligofructose, oligomeric galactose, soybean oligo saccharide, oligomeric xylose, oligomeric lactulose, oligomeric dragon gallbladder sugar, oligomeric isomaltose, palatinose, cyclodextrin, newborn sugarcane powder, stachyose, cottonseed sugar, manna oligosaccharide, trehalose, inulin or the inulin oligofructose;
Described sweeting agent comprises sugar sweetener, non-sugar sweetener or its mixture;
Described adjuvant comprises one or more in Chinese medicine extraction liquid or Chinese medicine powder, fruice and fruit, dry fruit extracting solution, natural food fibres, dry powder, tea powder, aminoacid, soybean lecithin, vitamin, Mel, pollen, trace element, flavorant, citric acid, Semen Maydis powder, Testa Tritici powder, soy milk powder, milk powder, coffee powder, egg albumen powder, oatmeal, black sesame powder, the drinking water;
Herbal species in described Chinese medicine extraction liquid or the Chinese medicine powder is to comprise in Radix Ginseng, Radix Angelicae Sinensis, Flos Caryophylli, Radix Polygoni Multiflori, Fructus Schisandrae Chinensis, Fructus Lycii, Flos Chrysanthemi, Semen Cassiae, Ganoderma, Radix Gentianae, Radix Salviae Miltiorrhizae, Radix Glycyrrhizae, Herba Menthae, the Radix Astragali and the Semen Nelumbinis one or more.
3. according to the described streptococcus faecium function signal molecule formulation of claim 2, it is characterized in that sugar sweetener in the described sweeting agent comprises sucrose, glucose, maltose, dextrin, dry powder Nulomoline, fructose, laevulose, galactose, primverose starch or with its molasses of making, syrup and their mixture; Described non-sugar sweetener comprises Sorbitol, mannitol, xylitol, hydrogenated starch hydrolysate, maltose alcohol, stevioside, aspartame, Flos Chrysanthemi essence, cyclamate and aspartame and their mixture.
4. according to claim 2 or 3 described streptococcus faecium function signal molecule formulations, it is characterized in that the described lowering blood-fat and reducing weight goods that contain based on the former powder of probiotic bacteria function signal molecular preparation of streptococcus faecium function signal molecule are powder, tablet, granule, water preparation, milk and milk powder, fruit juice.
5. according to the described streptococcus faecium function signal molecule formulation of claim 4, it is characterized in that the dosage form that described powder, granule are made is oral electuary, capsule or feed additive.
6. according to the described streptococcus faecium function signal molecule formulation of claim 4, it is characterized in that the dosage form that described water preparation, milk and milk powder, fruit juice are made is oral liquid, beverage, canned food.
7. the preparation method of streptococcus faecium function signal molecule formulation is characterized in that technical process is:
(1), streptococcus faecalis its thalline that ferments concentrates: the weight ratio that contains that the streptococcus faecalis seed liquor inserts after having sterilized is: soy peptone 0.5%～1.0%, show in the culture medium matrix of peptone 0.5%～1.0%, glucose 0.5%, serum powder 0.5%～1.0%, potassium dihydrogen phosphate 0.25%, Carnis Bovis seu Bubali cream 0.25%, Hepar Bovis seu Bubali powder 0.15%, yeast extract 0.5%～1.0%, sodium chloride 0.3%, soluble starch 0.5%, sodium thioglycollate 1.5%, behind 35～38 ℃ of anaerobic fermentations, promptly obtain streptococcus faecalis thalline concentrate through centrifugal concentrating again;
(2), cell wall breaking:, use high pressure cell disintegrating machine operating pressure 100 ± 10MPa with the broken somatic cells wall of high pressure with this streptococcus faecalis thalline concentrate adding distil water or normal saline (1: 3～1: 5);
(3), drying: till the solvent-free flavor, or the trehalose that adds 100～200ml carries out lyophilization as freeze drying protectant with these goods to cell extract in 40～70 ℃ of drying under reduced pressure.The dry thing that obtains promptly gets the former powder of streptococcus faecium function signal molecule formulation through grinding, sieving.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007101036918A CN101297820A (en) | 2007-04-30 | 2007-04-30 | Streptococcus faecium function signal molecule formulation and product thereof for reducing fat and slimming |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101971884A (en) * | 2010-09-10 | 2011-02-16 | 甘肃岷归中药材科技有限公司 | Micron Chinese angelica and Astragalus membranaceus instant soybean milk powder and preparation method thereof |
| CN102757922A (en) * | 2012-07-30 | 2012-10-31 | 陕西省科学院酶工程研究所 | Lactobacillus acidophilus composite freeze-drying protective agent and preparation method and usage method thereof |
| EP2581092A1 (en) * | 2010-06-08 | 2013-04-17 | Calpis Co., Ltd. | Lipid metabolism-improving agent |
| CN103392911A (en) * | 2013-08-06 | 2013-11-20 | 国家粮食局科学研究院 | Feed-use high-activity lactobacillus solid preparation and preparation method thereof |
| CN103461675A (en) * | 2013-09-23 | 2013-12-25 | 河南牧翔动物药业有限公司 | Livestock and poultry intestinal tract regulator and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2581092A1 (en) * | 2010-06-08 | 2013-04-17 | Calpis Co., Ltd. | Lipid metabolism-improving agent |
| EP2581092A4 (en) * | 2010-06-08 | 2013-12-18 | Calpis Co Ltd | Lipid metabolism-improving agent |
| US9468657B2 (en) | 2010-06-08 | 2016-10-18 | Asahi Group Holdings, Ltd. | Lactic acid bacterium agent for improving lipid metabolism |
| EP2581092B1 (en) | 2010-06-08 | 2017-04-05 | Asahi Group Holdings, Ltd. | Agent for improving lipid metabolism |
| CN101971884A (en) * | 2010-09-10 | 2011-02-16 | 甘肃岷归中药材科技有限公司 | Micron Chinese angelica and Astragalus membranaceus instant soybean milk powder and preparation method thereof |
| CN102757922A (en) * | 2012-07-30 | 2012-10-31 | 陕西省科学院酶工程研究所 | Lactobacillus acidophilus composite freeze-drying protective agent and preparation method and usage method thereof |
| CN103392911A (en) * | 2013-08-06 | 2013-11-20 | 国家粮食局科学研究院 | Feed-use high-activity lactobacillus solid preparation and preparation method thereof |
| CN103392911B (en) * | 2013-08-06 | 2015-03-18 | 国家粮食局科学研究院 | Feed-use high-activity lactobacillus solid preparation and preparation method thereof |
| CN103461675A (en) * | 2013-09-23 | 2013-12-25 | 河南牧翔动物药业有限公司 | Livestock and poultry intestinal tract regulator and preparation method thereof |
| CN103461675B (en) * | 2013-09-23 | 2015-05-20 | 河南牧翔动物药业有限公司 | Livestock and poultry intestinal tract regulator and preparation method thereof |
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