CN101294181B - Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane - Google Patents
Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane Download PDFInfo
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- CN101294181B CN101294181B CN2008101233978A CN200810123397A CN101294181B CN 101294181 B CN101294181 B CN 101294181B CN 2008101233978 A CN2008101233978 A CN 2008101233978A CN 200810123397 A CN200810123397 A CN 200810123397A CN 101294181 B CN101294181 B CN 101294181B
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- production
- fermentation
- oxygen carrier
- hyaluronic acid
- dodecane
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Abstract
A method for increasing the fermentation production of hyaluronic acid by adding an oxygen carrier of dodecane belongs to the technology field of applying the oxygen carrier to the optimization of the fermentation process. The invention adopts the method of adding the oxygen carrier of dedecane (1-5%, v/v) in a certain concentration to a fermentation broth after 8 hours' fermentation, so as to increase the dissolved oxygen concentration, thereby increasing the production of the hyaluronic acid. When the concentration of the dedecane is 5%, the production of the hyaluronic acid increases from 5.0g/L to 6.0g/L by contrast (no oxygen carrier is added). The method is simple, effective and applicable to the industrial production of the hyaluronic acid and has certain significance in guiding the optimization of other high-viscosity fermentation processes.
Description
Technical field
A kind of with the method for adding oxygen carrier n-dodecane raising hyaluronic acid volume of production of fermentation production, relate to oxygen carrier and add the applied technical field of strategy in optimizing fermentation.
Background technology
Hyaluronic acid is a kind of high-molecular weight mucopolysaccharide, and its two kinds of synthetic precursor substances are glucuronic acid and N-acetylglucosamine.Hyaluronic acid can extract from animal tissues such as cockscomb, also can pass through Production by Microorganism Fermentation.Because the fermentation method cost is low, product purity is high, microbe fermentation method just progressively replaces the tissue extraction method.Hyaluronic research mainly concentrates on Optimizing Conditions of Fermentation to Production by Microorganism Fermentation at present, as Optimum of culture medium, and the optimization of DESIGN OF REACTOR optimization and fermentation pattern.Hyaluronic fermentation belongs to high viscosity fermentation, and dissolved oxygen is the bottleneck that hyaluronic acid volume of production improves, and therefore, how effectively to improve the key issue that oxyty is the hyaluronic acid fermentation process optimization.Utilize the strategy that adds oxygen carrier to improve hyaluronic output, do not see bibliographical information as yet.
Summary of the invention
The purpose of this invention is to provide a kind of method, utilize the present invention can significantly improve hyaluronic output with the strategy raising hyaluronic acid volume of production of fermentation production that adds the oxygen carrier n-dodecane.
Technical scheme of the present invention: a kind of with the method for adding oxygen carrier n-dodecane raising hyaluronic acid volume of production of fermentation production, when fermentation 8h, add the oxygen carrier n-dodecane (1%-5% of certain volume percentage concentration, v/v) in fermented liquid, improve oxyty, and then improved hyaluronic output.Hyaluronic output improves with the raising of the concentration of n-dodecane, and when n-dodecane concentration was 5%, hyaluronic output was brought up to 6.0g/L by the 5.0g/L of contrast (not adding oxygen carrier).
Beneficial effect of the present invention: utilize the present invention can significantly improve hyaluronic acid volume of production.The raising of oxyty does not improve by improving mixing speed or Ventilation Rate, but the method for passing through to add cheap oxygen carrier n-dodecane, so the energy consumption reduction, production cost also decreases.In addition, provided by the present invention have certain guidance and edify meaning other high viscosity fermentation by adding strategy that oxygen carrier improves oxyty.
Embodiment
Comparative examples: the used bacterial classification of the present invention is streptococcus zooepidemicus Streptococcus zooepidemicusWSH-24, (or being designated as H-24) (Wen Qi, Liu Dengru, Chen Jian, stifled state become " add tensio-active agent and promote streptococcus zooepidemicus high yield hyaluronic acid ", " chemical industry progress " 2006,25 (9), 1089～1094; Chinese patent 200610098195.3 is open), changeing the inclined-plane earlier cultivated 12 hours in 37 ℃ of incubators, carried out shake flask fermentation then 14 hours, 37 ℃ of temperature, rotating speed 200rpm fermented in the 7L fermentor tank 16 hours at last, and fermentation condition is: pH 7.0, mixing speed 200rpm, Ventilation Rate 1.0vvm.Fermention medium is (g/L): sucrose: 70; Yeast powder: 25; MgSO
47H
2O:2.0; Na
2HPO
4: 6.3; K
2SO
4: 7.0.Hyaluronic molecular weight is 1,300,000, and output is 5.0g/L.
Embodiment 1: ferment tank adopts oxygen carrier to add strategy, the same comparative examples of all the other conditions.Interpolation oxygen carrier n-dodecane during fermentation 8h (1%, v/v).The result: hyaluronic acid volume of production is brought up to 5.2g/L by the 5g/L of contrast.
Embodiment 2: ferment tank adopts oxygen carrier to add strategy, the same comparative examples of all the other conditions.Interpolation oxygen carrier n-dodecane during fermentation 8h (3%, v/v).The result: hyaluronic acid volume of production is brought up to 5.6g/L by the 5g/L of contrast.
Embodiment 3: ferment tank adopts oxygen carrier to add strategy, the same comparative examples of all the other conditions.Interpolation oxygen carrier n-dodecane during fermentation 8h (5%, v/v).The result: hyaluronic acid volume of production is brought up to 6.0g/L by the 5g/L of contrast.
Claims (1)
1. one kind is improved the method for hyaluronic acid volume of production of fermentation production with adding the oxygen carrier n-dodecane, it is characterized in that when fermentation 8h, adding the oxygen carrier n-dodecane of 1%-5% concentration expressed in percentage by volume in streptococcus zooepidemicus (Streptococcus zooepidemicus) WSH-24 fermented liquid, with the raising oxyty, and then improve hyaluronic output.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101233978A CN101294181B (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101233978A CN101294181B (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101294181A CN101294181A (en) | 2008-10-29 |
| CN101294181B true CN101294181B (en) | 2010-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101233978A Expired - Fee Related CN101294181B (en) | 2008-05-26 | 2008-05-26 | Method for improving hyaluronic acid volume of production of fermentation production by adding oxygen carrier dodecane |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110551784A (en) * | 2019-09-18 | 2019-12-10 | 宁夏泰益欣生物科技有限公司 | Fermentation method for increasing content of abamectin B1a |
| CN112458309A (en) * | 2020-12-14 | 2021-03-09 | 昆明冶金研究院有限公司 | Method for improving zinc leaching rate in zinc sulfide concentrate |
| CN115960979A (en) * | 2022-12-30 | 2023-04-14 | 华熙生物科技股份有限公司 | Method for producing hyaluronic acid or salt thereof by fermentation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1978658A (en) * | 2006-12-08 | 2007-06-13 | 江南大学 | Method for improving fermentation-produced hyaluronicacid yield using intermittent high pH tolerant strategy |
-
2008
- 2008-05-26 CN CN2008101233978A patent/CN101294181B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1978658A (en) * | 2006-12-08 | 2007-06-13 | 江南大学 | Method for improving fermentation-produced hyaluronicacid yield using intermittent high pH tolerant strategy |
Non-Patent Citations (2)
| Title |
|---|
| 成霞等.高产量_高分子量透明质酸发酵条件优化.《过程工程学报》.2006,第6卷(第5期),第809-813页. * |
| 温琦等.添加表面活性剂促进兽疫链球菌高产透明质酸.《化工进展》.2006,第25卷(第9期),第1089-1094页. * |
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| CN101294181A (en) | 2008-10-29 |
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