CN101273747B - 米糠蛋白提取工艺 - Google Patents
米糠蛋白提取工艺 Download PDFInfo
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- CN101273747B CN101273747B CN2008100475560A CN200810047556A CN101273747B CN 101273747 B CN101273747 B CN 101273747B CN 2008100475560 A CN2008100475560 A CN 2008100475560A CN 200810047556 A CN200810047556 A CN 200810047556A CN 101273747 B CN101273747 B CN 101273747B
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- rice bran
- protein
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 93
- 235000009566 rice Nutrition 0.000 title claims abstract description 93
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 81
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 81
- 238000000034 method Methods 0.000 title claims abstract description 27
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 92
- 229930014626 natural product Natural products 0.000 claims abstract description 11
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 244000068988 Glycine max Species 0.000 claims description 65
- 235000010469 Glycine max Nutrition 0.000 claims description 64
- 239000002994 raw material Substances 0.000 claims description 40
- 235000013336 milk Nutrition 0.000 claims description 24
- 239000008267 milk Substances 0.000 claims description 24
- 210000004080 milk Anatomy 0.000 claims description 24
- 239000002002 slurry Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 13
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000000284 extract Substances 0.000 claims description 10
- 210000000997 thin albumen Anatomy 0.000 claims description 10
- 238000009835 boiling Methods 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 5
- 101710094902 Legumin Proteins 0.000 claims description 4
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000005303 weighing Methods 0.000 claims description 2
- FODTZLFLDFKIQH-FSVGXZBPSA-N gamma-Oryzanol (TN) Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)O[C@@H]2C([C@@H]3CC[C@H]4[C@]5(C)CC[C@@H]([C@@]5(C)CC[C@@]54C[C@@]53CC2)[C@H](C)CCC=C(C)C)(C)C)=C1 FODTZLFLDFKIQH-FSVGXZBPSA-N 0.000 abstract description 12
- 235000013305 food Nutrition 0.000 abstract description 9
- 235000015097 nutrients Nutrition 0.000 abstract description 6
- 108010082495 Dietary Plant Proteins Proteins 0.000 abstract description 2
- 239000012535 impurity Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 4
- 238000003916 acid precipitation Methods 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 238000006911 enzymatic reaction Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 34
- 239000000523 sample Substances 0.000 description 18
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 15
- 235000008696 isoflavones Nutrition 0.000 description 15
- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 8
- 238000002372 labelling Methods 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 8
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 150000002515 isoflavone derivatives Chemical class 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- 238000011084 recovery Methods 0.000 description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
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- 101100020212 Mus musculus Klhdc3 gene Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 229930003427 Vitamin E Natural products 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 2
- 238000002386 leaching Methods 0.000 description 2
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 235000017807 phytochemicals Nutrition 0.000 description 2
- 229930000223 plant secondary metabolite Natural products 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 229940071440 soy protein isolate Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229940046009 vitamin E Drugs 0.000 description 2
- 235000019165 vitamin E Nutrition 0.000 description 2
- 239000011709 vitamin E Substances 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- RLLPVAHGXHCWKJ-UHFFFAOYSA-N permethrin Chemical compound CC1(C)C(C=C(Cl)Cl)C1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 RLLPVAHGXHCWKJ-UHFFFAOYSA-N 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Landscapes
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
Description
成份 | 含量 | 测定方法(见下段) |
黄豆蛋白含量 | 52.345±0.222mg/g原料(黄豆+米糠,1∶4,w/w) | 考马斯亮蓝蛋白测定试剂盒 |
米糠蛋白含量 | 105.010±2.634mg/g原料(黄豆+米糠,1∶4,w/w) | 考马斯亮蓝蛋白测定试剂盒 |
米糠蛋白提取率 | 131.269±3.292mg/g(米糠) | 考马斯亮蓝蛋白测定试剂盒 |
γ-谷维醇(γ-oryzanols) | 2.350±0.108mg/g原料(黄豆+米糠,1∶4,w/w) | 按照论文(余尚工,方念伯.Quantification ofVitamin E and γ-Oryzanol ComponentsinRice Germ and Bran Using LC-MS/MS.J.Agric.Food Chem.2007,55:7308-7313.)所描述的方法,来测定oryzanol含量. |
总异黄酮(isoflavones) | 0.156±0.009mg/g原料(黄豆+米糠,1∶4,w/w) | 按照论文(方念伯,余尚工.Comprehensivephytochemical profile of soy protein isolate.J.Agric.Food Chem.2004,52:4012-4020)所描述的方法,来测定isoflavone含量 |
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CN2008100475560A CN101273747B (zh) | 2008-05-05 | 2008-05-05 | 米糠蛋白提取工艺 |
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CN2008100475560A CN101273747B (zh) | 2008-05-05 | 2008-05-05 | 米糠蛋白提取工艺 |
Publications (2)
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CN101273747A CN101273747A (zh) | 2008-10-01 |
CN101273747B true CN101273747B (zh) | 2012-02-15 |
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CN2008100475560A Expired - Fee Related CN101273747B (zh) | 2008-05-05 | 2008-05-05 | 米糠蛋白提取工艺 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103082171A (zh) * | 2011-10-31 | 2013-05-08 | 余尚学 | 米糠γ-谷维醇和复合维生素E营养成分的提取工艺 |
CN103099097A (zh) * | 2011-11-14 | 2013-05-15 | 余尚学 | 米糠中蛋白质、γ -谷维醇和维生素E复合营养成分的提取工艺 |
CN113057291A (zh) * | 2019-12-12 | 2021-07-02 | 杭州九阳豆业有限公司 | 一种保健豆浆粉及其制备工艺 |
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2008
- 2008-05-05 CN CN2008100475560A patent/CN101273747B/zh not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
陈正行.米糠浓缩蛋白的提取及功能性评价.《食品与发酵工业》.2000,第26卷(第4期),17-19. * |
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