CN101273708A - Molecular-assisted jujube tree artificial control crossbreeding method - Google Patents
Molecular-assisted jujube tree artificial control crossbreeding method Download PDFInfo
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- CN101273708A CN101273708A CNA2008100003733A CN200810000373A CN101273708A CN 101273708 A CN101273708 A CN 101273708A CN A2008100003733 A CNA2008100003733 A CN A2008100003733A CN 200810000373 A CN200810000373 A CN 200810000373A CN 101273708 A CN101273708 A CN 101273708A
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- jujube tree
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- 244000126002 Ziziphus vulgaris Species 0.000 title claims abstract description 33
- 235000008529 Ziziphus vulgaris Nutrition 0.000 title claims abstract description 32
- 238000000034 method Methods 0.000 title claims abstract description 17
- 238000009402 cross-breeding Methods 0.000 title claims abstract description 15
- 241000238631 Hexapoda Species 0.000 claims abstract description 17
- 230000010152 pollination Effects 0.000 claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 14
- 238000009395 breeding Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 10
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 claims abstract description 4
- 238000011156 evaluation Methods 0.000 claims abstract description 4
- 238000009394 selective breeding Methods 0.000 claims abstract description 3
- 238000009396 hybridization Methods 0.000 claims description 8
- 230000008774 maternal effect Effects 0.000 claims description 8
- 241000256844 Apis mellifera Species 0.000 claims description 7
- 238000002372 labelling Methods 0.000 claims description 4
- 239000006188 syrup Substances 0.000 claims description 3
- 235000020357 syrup Nutrition 0.000 claims description 3
- 235000006545 Ziziphus mauritiana Nutrition 0.000 claims description 2
- 230000000295 complement effect Effects 0.000 claims description 2
- 239000000284 extract Substances 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000009423 ventilation Methods 0.000 claims description 2
- 239000004575 stone Substances 0.000 claims 1
- 241001247821 Ziziphus Species 0.000 abstract description 8
- 238000009331 sowing Methods 0.000 abstract description 3
- 239000003147 molecular marker Substances 0.000 abstract 2
- 230000009897 systematic effect Effects 0.000 abstract 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 abstract 1
- 239000004677 Nylon Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000010396 two-hybrid screening Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Abstract
A molecular-assisted jujube tree artificial control cross breeding method includes such steps as artificial selection of parents, cover net control cross, auxiliary pollination by insects, molecular marker hybrid identification, etc. to obtain jujube tree hybrid progeny. According to a breeding target, parents are selected and matched, and the two parents are adjacently planted or grafted on the same jujube tree; before the parent plants bloom, covering all the parent trees by using a net which is ventilated and light-permeable but can prevent pollinating insects from entering, putting the pollinating insects which do not collect jujube pollen into the net to help pollination, and removing the pollinating insects and the net cover after the flowering period is finished; taking off all the fruits of the two parents, taking out kernels, and sowing to obtain seedlings; extracting genome DNA of each seedling and two parents, and performing hybrid identification by molecular marker technology such as AFLP; and performing systematic observation comparison and evaluation on the hybrid progeny seedlings, and breeding excellent varieties. The method is applied to jujube tree breeding practice, can break through the bottleneck of difficult emasculation in jujube tree crossbreeding, and improves jujube tree germplasm innovation and crossbreeding efficiency.
Description
Technical field
The present invention relates to the auxiliary jujube tree Artificial Control cross breeding method of a kind of molecule.
Background technology
Crossbreeding can make male parent and maternal recombination of genes realize germplasm innovation, is one of method the most commonly used in the fruit breeding.But on jujube tree, owing to spend little (diameter is only about 0.5cm), artificial emasculation difficulty, jujube tree nature fruit-setting rate is very low and the embryo abortion is serious in addition, causes being difficult to obtain the hybrid generation after the artificial hybridization.Domestic and international how tame R﹠D institution has successively carried out a large amount of jujube tree artificial pollinations and has put into practice (artificial emasculation, artificial pollination, bagging), but seldom obtains hybrid generation colony, does not cultivate new varieties by artificial hybridization as yet both at home and abroad on jujube tree so far.At present, the germplasm innovation work of jujube tree mainly is that the directionality of breeding is relatively poor by seed selection and the mutation breeding etc. of naturally growing directly from seeds, and breeding efficiency is lower.Therefore, the exploitation efficient jujube tree crossbreeding technology that can obtain the hybrid generation in a large number becomes the task of top priority.
Jujube flower is typical entomophilous flower.The pollen grain viscosity of jujube is big, can not pollinate by wind-force, mainly pollinates by insect pollinators such as honeybees under the natural conditions.If adjacent male parent and female parent are covered the net processing, and in net, put honeybee, be expected under the situation that effectively stops foreign pollen to be imported into, to realize artificial controlled hybridization pollination, and then filial generation identified in early days to determine hybrid by molecular marking technique.
Summary of the invention
The present invention has set up the auxiliary jujube tree Artificial Control cross breeding method of a kind of molecule, helps making up and obtaining the hybrid generation expeditiously by artificial selection father, female parent, accelerates jujube tree crossbreeding process.
The technical solution adopted for the present invention to solve the technical problems is: at first select desirable hybrid strain, with two parents (male parent and female parent) adjacent plant or grafting on same strain jujube tree, with net parents are covered realization and the isolation of other jujube tree at the florescence, put insect pollinator (honeybee etc.) in the net and promote pollination, florescence removes insect pollinator and net after finishing, in time gather fruit on the parent tree, strip kind of a benevolence, begin sowing in good time, utilize molecular marking technique to carry out hybrid identification, keep hybrid plant, further estimate.
Particular content of the present invention, i.e. the method for the auxiliary jujube tree Artificial Control crossbreeding of molecule is as follows:
(1) selects suitable male parent and female parent according to breeding objective, general selection Comprehensive Traits is good and kernel-containing rate is high, plant the full kind of benevolence or major clique is done maternal, having complementary merit with female parent and pollen amount is big, Pollen Activity is high, pollination affinity strong kind or major clique made male parent, with two parents (each other male parent and female parent) adjacent plant or grafting on same strain jujube tree;
(2) before two parental plants are about to enter the florescence, with its with ventilation and penetrating light but the net that can stop insect pollinator to enter all cover, in net, put into the insect pollinator of not gathering Chinese date pollen (such as honeybee etc.) and help pollination hybridization, in net, put into the pollination time that syrup prolongs insect pollinator simultaneously, full-bloom stage removes insect pollinator after finishing, and removes guard after the florescence finishes;
After (3) two parents go up the fruit maturation, all take off respectively, take out complete kind of benevolence;
(4) will plant benevolence and sow respectively the acquisition seedling, seedling divides individual plant to extract genomic DNA, extracts simultaneously two parents' genomic DNA, adopts AFLP or RAPD equimolecular labelling technique, determines whether each strain seedling is hybrid seedling;
(5) hybrid seedling is carried out system's observation and comparison and evaluation, the seed selection improved seeds.
The beneficial effect of patent of the present invention is, by the cover net two hybrid strains and other jujube trees are isolated, by the auxiliary pollination of insect pollinator, utilize at last whether molecular marking technique is the scientific verification of hybrid to filial generation, can effectively solve like this in the jujube tree crossbreeding because jujube flower is little, it is very difficult to castrate and hinder the problem that flower causes being difficult to obtain a large amount of hybrid generations easily.This technology is applied to the jujube tree breeding practice, can break through and castrate difficult bottleneck in the jujube tree crossbreeding, greatly accelerates jujube tree germplasm innovation progress, improves crossbreeding efficient.
Description of drawings
Fig. 1 covers the situation of putting the honeybee supplementary pollination in net and the net to two parents.
Fig. 2 is the cover network control system hybridization maternal result's of going up in back a situation.
After Fig. 3 was the jujube tree physiological fallen fruit, the maternal fruit different developmental phases of control hybridization contained the situation of benevolence.
Embodiment
Be the good seedless Zizyphus jujuba Mill kind of seed selection, select a strain to become winter in age jujube (have nuclear, kernel-containing rate height, fresh food quality are extremely gone up, Comprehensive Traits good) for maternal, the seedless little jujube of a strain that is adjacent (plant nuclear is degenerated, fruit can all eat) is male parent; Mid-May, maternal and male parent set enter florescence before, with two parents with white nylon wire (25 holes/cm
2) all cover; In net, put into the honeybee of not gathering other jujube Varieties in Pollen Morphology 1 case after entering the florescence, and in net, put into a certain amount of syrup (white sugar: water=1: 1); Full-bloom stage removes honeybee after finishing, and removes the nylon wire cover after the florescence end; After maternal fruit full maturity, take the jujube fruit, take out jujube nuclear, careful allocation kind benevolence, sowing obtains seedling 156 strains; Get the young leaflet tablet of male parent, female parent and each seedling, adopt the AFLP molecule labelling method, identify hybrid seedling 87 strains; Hybrid generation to determining has entered the field evaluation stage.
Claims (2)
1. the jujube tree Artificial Control cross breeding method that molecule is auxiliary is characterized in that obtaining the hybrid generation by artificial selection parent, the hybridization of cover network control system, the auxiliary pollination of insect, molecular labeling hybrid identification etc.
2. in accordance with the method for claim 1, it is characterized in that comprising following step:
(1) select suitable male parent and female parent according to breeding objective, with two parents (each other male parent and female parent) adjacent plant or grafting on same strain jujube tree;
(2) before two parental plants are about to enter the florescence, with its with ventilation and penetrating light but the net that can stop insect pollinator to enter all cover, in net, put into the insect pollinator of not gathering Chinese date pollen (such as honeybee etc.) and help pollination hybridization, in net, put into the pollination time that syrup prolongs insect pollinator simultaneously, full-bloom stage removes insect pollinator after finishing, and removes guard after the florescence finishes;
(3) two parents that in time gather go up fruit, strip out fruit stone, therefrom take out complete kind of benevolence;
(4) will plant benevolence and sow respectively the acquisition seedling, seedling divides individual plant to extract genomic DNA, extracts simultaneously two parents' genomic DNA, adopts AFLP, RAPD equimolecular labelling technique, determines whether each strain seedling is hybrid;
(5) hybrid seedling is carried out system's observation and comparison and evaluation, the seed selection improved seeds.
3. in accordance with the method for claim 1, it is characterized in that selecting comprehensive proterties good and kernel-containing rate is high, plant the full kind of benevolence or major clique do maternal, to have complementary merit with female parent and pollen amount is big, pollen vigor height, kind or major clique that pollination affinity is strong are made male parent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100003733A CN101273708B (en) | 2008-01-09 | 2008-01-09 | Molecular-assisted jujube tree artificial control crossbreeding method |
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| CN2008100003733A CN101273708B (en) | 2008-01-09 | 2008-01-09 | Molecular-assisted jujube tree artificial control crossbreeding method |
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| Publication Number | Publication Date |
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| CN101273708A true CN101273708A (en) | 2008-10-01 |
| CN101273708B CN101273708B (en) | 2012-07-18 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052566A (en) * | 2015-08-18 | 2015-11-18 | 大连民族大学 | Molecular designing method for tea-oil tree high-yield top grafting and crown changing |
| CN106665329A (en) * | 2016-12-12 | 2017-05-17 | 河北农业大学 | Method for large-scale control hybridization of jujube trees by using simple assembly net |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
| CN110150139A (en) * | 2019-06-14 | 2019-08-23 | 塔里木大学 | A method of moveable convenient jujube cross combination |
| CN115777525A (en) * | 2022-12-20 | 2023-03-14 | 商丘市农林科学院 | Peanut crossbreeding marking method |
-
2008
- 2008-01-09 CN CN2008100003733A patent/CN101273708B/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105052566A (en) * | 2015-08-18 | 2015-11-18 | 大连民族大学 | Molecular designing method for tea-oil tree high-yield top grafting and crown changing |
| CN106665329A (en) * | 2016-12-12 | 2017-05-17 | 河北农业大学 | Method for large-scale control hybridization of jujube trees by using simple assembly net |
| CN107135942A (en) * | 2017-06-04 | 2017-09-08 | 滨州市沾化区冬枣实业总公司 | A kind of breeding method of triploid winter jujube |
| CN110150139A (en) * | 2019-06-14 | 2019-08-23 | 塔里木大学 | A method of moveable convenient jujube cross combination |
| CN115777525A (en) * | 2022-12-20 | 2023-03-14 | 商丘市农林科学院 | Peanut crossbreeding marking method |
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| Publication number | Publication date |
|---|---|
| CN101273708B (en) | 2012-07-18 |
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Granted publication date: 20120718 Termination date: 20130109 |