CN101264067A - Florfenicol capsule for silkworm - Google Patents
Florfenicol capsule for silkworm Download PDFInfo
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- CN101264067A CN101264067A CNA2008100255325A CN200810025532A CN101264067A CN 101264067 A CN101264067 A CN 101264067A CN A2008100255325 A CNA2008100255325 A CN A2008100255325A CN 200810025532 A CN200810025532 A CN 200810025532A CN 101264067 A CN101264067 A CN 101264067A
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Abstract
The invention provides a florfenicol capsule for silkworm. The florfenicol technical powder is mixed evenly with solubilization addition agent at a matching of 1 to 2 to 3 before measuring and encapsuling. When using, the materials in the capsule are dissolved by water and blended evenly in order to obtain medical solution with concentration of 50 to 100 Mug/ml. The medical solution is sprayed on the mulberry leaves at weight ratio of 1 to 10 to 20. 3-instar silkworms lick once pre day, 4-instar silkworms lick once to twice pre day, 5-instar silkworms lick three times to four times pre day since first feeding. When sick silkworm appears, the medical solution is licked once per eight hours until recovering. The florfenicol capsule has the advantages of good control effect to bacteriosis for silkworm, simple production technology, low cost, none environmental pollution and residue, good improvements of quality and yield of cocoon and good economic and social benefits.
Description
Technical field
The present invention relates to a kind of silkworm medicine, particularly a kind of florfenicol capsule for silkworm.
Background technology
In the sericulture production process, the harm of flacherie is very serious, and particularly the destruction of silkworm bacterial disease makes the silkworm raiser be difficult to control aborning, causes the significantly underproduction of Bombyx bombycis, and even does not have receipts, and the reduction of silk matter is badly damaged silkworm raiser's interests.Therefore, the control of flacherie is the important step in the sericulture.At present, medical treatment is the main means of silkworm bacterial disease control.And the significant silkworm of prevention effect of using over a long period of time has been put into the animal forbidden drugs with chloromycetin, now adopt norfloxacin and erythromycin usually, but because the erythromycin water solublity is relatively poor, quality is unstable, prevention effect is not satisfactory, is difficult to apply aborning; Norfloxacin is then relatively poor because of the prevention effect of self, is difficult to reach the purpose of killing the silkworm antibacterial.
Florfenicol is called Florfenicol again, belong to broad ectrum antibiotic, have widely distributed, the no potential induced aplastic anemia effect in good, the body that absorbs, characteristics such as safe in utilization, successively register in states such as Japan, Norway, France, Britain, Austria, Mexico and Spain, China is in April, 1994 approval listing, for country-level new veterinary drug, now be mainly used in the bacterial disease of treatment by Ministry of Agriculture's approval by the microbial pig of sensitivity, cattle, chicken and fish.
Summary of the invention
Technical problem to be solved by this invention is at the deficiencies in the prior art, provides that a kind of prevention effect is good, production technology is simple, cost is low, the florfenicol capsule for silkworm of non-environmental-pollution, to strengthen silkworm bacterial disease prevention effect.
The technical solution adopted for the present invention to solve the technical problems is: comprise former powder of florfenicol and solubilisation aids, the proportioning with 1: 2～3 is behind the mix homogeneously, in metering incapsulates.
Described solubilisation aids is a glucose powder.
The proportioning of former powder of above-mentioned florfenicol and solubilisation aids is 3: 7.
Florfenicol capsule for silkworm of the present invention, its using method is: capsule 's content is dissolved in the ordinary water, stir well, be mixed with the medicinal liquid of 50～100 μ g/ml concentration, weight ratio with 1: 10～20 is sprayed on the Folium Mori blade face, three age the silkworm first feeding rise and add food every day 1 time, four age silkworm add food every day 1～2 time, five age silkworm add food 3～4 times; When finding the disease silkworm, added food 1 time, freshen food continuously, till recovering every 8 hours.
Florfenicol capsule for silkworm of the present invention prevents and therapeutic test silkworm common bacterial septicemia:
One, test specimen:
Florfenicol capsule for silkworm is mixed with 500 μ g/ml, 200 μ g/ml, 100 μ g/ml, 50 μ g/ml, 25 μ g/ml;
The former powder of chloromycetin is mixed with 1000 μ g/ml, 500 μ g/ml, 200 μ g/ml, 100 μ g/ml, 50 μ g/ml;
The former powder of erythromycin is mixed with 500 μ g/ml, 200 μ g/ml, 100 μ g/ml, 50 μ g/ml.
Two, experimental animal:
Mountain valley with clumps of trees and bamboo silkworm: mountain valley with clumps of trees and bamboo pine * bright moon (company provides by Jiangsu Province's silkworm egg) is hybridized 3～4 days 5 ages.
Antibacterial: switching was 1 time before septicaemia bacterium, a blue or green deteriorated blood pathogenic bacteria (preserving rejuvenation by University Of Suzhou silkworm and mulberry institute physiological and pathological research department) were used.Strain of bacterium liquid preparation adds sterilized water 20ml, stirs evenly standbyly, and culture medium is the Carnis Bovis seu Bubali cream bacteria culture media.
Three, test method and result:
1, extracorporeal bacteria inhibitor test.
Method: culture medium is dissolved to not scalding nose, add bacterium liquid, cast flat board, (d=3mm) is placed on the flat board with circular hole filter paper, every quantitatively adds variable concentrations medicament 5.0 μ l, cultivates hour for 28 ℃, measures bacteriostatic diameter, the result shows (seeing Table 1): each concentration of treatment of florfenicol all has bacteriostasis to bacterial disease, and its fungistatic effect is better than erythromycin and chloromycetin.
Table 1: florfenicol capsule for silkworm is to the inhibition test of bacteremic septicemia bacterium
2, prophylactic tria.
Add the food silkworm, edible 8 hours continuously, stop administration, respectively at 0 hour, 2 hours, 4 hours, 8 hours, puncture inoculation septicaemia bacterium, a blue or green deteriorated blood pathogenic bacteria were raised 48 hours for 28 ℃, the investigation sickness rate, result's following (seeing Table 2,3,4,5): each concentration of treatment of florfenicol all has preventive effect to bacterial disease, and it is lower than erythromycin to a blue or green septicemic cemia preventive effect, and a blue or green septicemic cemia prevention effect then is better than erythromycin significantly; When its concentration was 100 μ g/ml, promptly the preventive effect with the chloromycetin of 500 μ g/ml concentration was suitable to the prevention of septicaemia; And when its concentration is 50 μ g/ml, a blue or green septicemic cemia preventive effect promptly is better than the chloromycetin of 500 μ g/ml concentration.
Table 2: add food 0 hour preventive effect at interval to bacterial disease
Table 3: add food 2 hours preventive effects at interval to bacterial disease
Table 4: add food 4 hours preventive effects at interval to bacterial disease
Table 5: add food 8 hours preventive effects at interval to bacterial disease
3, therapeutic test
Septicaemia bacterium, a blue or green deteriorated blood pathogenic bacteria are inoculated in puncture respectively, 28 ℃ of raisings, and (treatment concentration: silkworm is used florfenicol 150 μ g/ml, 100 μ g/ml, 50 μ g/ml in adding food antibiotics at interval in 0 hour, 2 hours, 4 hours, 8 hours respectively; Chloromycetin 1000 μ g/ml; Erythromycin 100 μ g/ml.), raised 48 hours, the investigation sickness rate, result's following (seeing Table 6,7,8,9): each concentration of treatment of florfenicol is to antibacterial and sick all tool excellent curative, and can realize the zero sickness rate of septicaemia and septicaemia when concentration only is 50 μ g/ml.
Table 6: inoculate 0 hour therapeutic effect to bacterial disease
Table 7: inoculate 2 hours therapeutic effect to bacterial disease
Table 8: inoculate 4 hours therapeutic effect to bacterial disease
Table 9: inoculate 8 hours therapeutic effect to bacterial disease
Florfenicol capsule for silkworm of the present invention is tested the influence of silkworm economic indicator:
One, material: florfenicol capsule for silkworm is mixed with 500 μ g/ml concentration with distilled water.
Two, silkworm rearing: establish two districts, one district is for adding the medicine district, another district is for adding the water check plot, every district raises 100 of silkworms, plays silkworm 3 ages and begins to add continuously every day food 500 μ g/ml florfenicol capsule for silkworm solution, adds food 8 hours to 5 every days in age, the food clear water is freshened in the check plot, raise and go up a hill, every index is investigated in the production of hybrid seeds.
Three, to the influence of economic indicator: with the Bombyx bombycis of results, peel off husks, abundant mixing, be divided into two, the parameter of 50 Bombyx bombycis of investigation, result's following (seeing Table 10): 500 μ g/ml florfenicol capsule for silkworm solution not only help the growth promoter of silkworm, and can improve Bombyx bombycis output and quality.
Table 10: florfenicol capsule for silkworm is to the influence of silkworm economic indicator
| Economic indicator | Silkworm is used florfenicol | CK |
| Dead cage rate (%) | 2.1 | 2.1 |
| Cocoon weight (gram) | 1.62 | 1.61 |
| Cocoon shell weight (gram) | 0.409 | 0.408 |
| Cocoon layer rate (%) | 25.25 | 25.34 |
| Good ovum number (grain) | 471 | 474 |
| Output ovum number (grain) | 485 | 490 |
| Good ovum rate (%) | 97.11 | 96.73 |
Above-mentioned test shows, florfenicol capsule for silkworm of the present invention has good prevention effect to bacterial disease of domestic silkworm, and production technology is simple, cost is low, non-environmental-pollution and residual, helps improving the quality and yield of Bombyx bombycis, has good economic benefit and social benefit.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment one:
Extract the former powder of 75g florfenicol and 175g glucose powder respectively by 3: 7 weight ratios, behind the mix homogeneously, be sub-packed in the capsule, make 1000 florfenicol capsule for silkworm by the metering of 250mg as solubilisation aids.
During use, get 1 above-mentioned capsule, its content is dissolved in the water of 1000ml, stir, make 75 μ g/ml medicinal liquids, be sprayed on the Folium Mori blade face of 15Kg, three age the silkworm first feeding rise and add food every day 1 time, four age silkworm add food every day 1～2 time, five age silkworm add food 3～4 times every day; When finding the disease silkworm, added food 1 time, freshen food continuously, till recovering every 8 hours.
Adopt above-mentioned florfenicol capsule for silkworm and using method, red strong, HONGGUANG village has selected eight family silkworm raisers to carry out actual culture experiment in Dongtai City, Jiangsu Province, comparative test result (seeing Table 11) with 75 μ g/ml florfenicol capsule for silkworm medicinal liquids and 500 μ g/ml norfloxacin medicinal liquids, 100 μ g/ml erythromycin medicinal liquids: the silkworm of adopting the control of florfenicol medicinal liquid all is better than the silkworm of norfloxacin medicinal liquid and erythromycin medicinal liquid control on indexs such as four-moulter body weight, matured silkworm body weight, the amount of cocoon production; On the septicemia rate, then be starkly lower than the silkworm of norfloxacin medicinal liquid and erythromycin medicinal liquid control.
Table 11: actual culture experiment synopsis
Embodiment two:
Extract the former powder of 75g florfenicol and 187.5g glucose powder respectively by 3: 7.5 weight ratios, behind the mix homogeneously, be sub-packed in the capsule, make florfenicol capsule for silkworm by the metering of 250mg as solubilisation aids.
During use, get 1 above-mentioned capsule, its content is dissolved in the water of 1000ml, stir, make the medicinal liquid of 71.4 μ g/ml concentration, be sprayed on the Folium Mori blade face of 15Kg, three age the silkworm first feeding rise and add food every day 1 time, four age silkworm add food every day 1～2 time, five age silkworm add food 3～4 times every day; When finding the disease silkworm, added food 1 time, freshen food continuously, till recovering every 8 hours.
Adopt above-mentioned florfenicol capsule for silkworm and using method, red strong, HONGGUANG village has also selected eight family silkworm raisers to carry out actual culture experiment in Dongtai City, Jiangsu Province, and with the comparative test result of 71.4 μ g/ml florfenicol capsule for silkworm medicinal liquids and 500 μ g/ml norfloxacin medicinal liquids, 100 μ g/ml erythromycin medicinal liquids, substantially with embodiment one.
Claims (4)
1, florfenicol capsule for silkworm is characterized in that: the proportioning of former powder of florfenicol and solubilisation aids 1: 2～3, and behind the mix homogeneously, in metering incapsulates.
2, florfenicol capsule for silkworm according to claim 1 is characterized in that: the proportioning of former powder of above-mentioned florfenicol and solubilisation aids is 3: 7.
3, florfenicol capsule for silkworm according to claim 1 and 2 is characterized in that: described solubilisation aids is a glucose powder.
4, florfenicol capsule for silkworm according to claim 1, it is characterized in that: during use, capsule 's content is dissolved in the ordinary water, stir well, be mixed with the medicinal liquid of 50～100 μ g/ml concentration, the weight ratio with 1: 10～20 is sprayed on the Folium Mori blade face, three age the silkworm first feeding rise add every day the food 1 time, four age silkworm add food every day 1～2 time, five age silkworm add food 3～4 times every day; When finding the disease silkworm, added food 1 time, freshen food continuously, till recovering every 8 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100255325A CN101264067A (en) | 2008-05-07 | 2008-05-07 | Florfenicol capsule for silkworm |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100255325A CN101264067A (en) | 2008-05-07 | 2008-05-07 | Florfenicol capsule for silkworm |
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| CN101264067A true CN101264067A (en) | 2008-09-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2008100255325A Pending CN101264067A (en) | 2008-05-07 | 2008-05-07 | Florfenicol capsule for silkworm |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919588A (en) * | 2012-11-02 | 2013-02-13 | 韩益飞 | Flacherie-preventing feed |
| CN103828841A (en) * | 2014-03-18 | 2014-06-04 | 华南农业大学 | Medicinal composition for preventing and controlling pebrine and application of medicinal composition |
-
2008
- 2008-05-07 CN CNA2008100255325A patent/CN101264067A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102919588A (en) * | 2012-11-02 | 2013-02-13 | 韩益飞 | Flacherie-preventing feed |
| CN103828841A (en) * | 2014-03-18 | 2014-06-04 | 华南农业大学 | Medicinal composition for preventing and controlling pebrine and application of medicinal composition |
| CN103828841B (en) * | 2014-03-18 | 2015-09-30 | 华南农业大学 | A kind of pharmaceutical composition and application thereof preventing and treating pebrine disease |
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Open date: 20080917 |