CN101259197A - Medicinal composition for treating cold and preparation thereof - Google Patents

Medicinal composition for treating cold and preparation thereof Download PDF

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Publication number
CN101259197A
CN101259197A CNA2007100797610A CN200710079761A CN101259197A CN 101259197 A CN101259197 A CN 101259197A CN A2007100797610 A CNA2007100797610 A CN A2007100797610A CN 200710079761 A CN200710079761 A CN 200710079761A CN 101259197 A CN101259197 A CN 101259197A
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parts
radix
extraction
flos lonicerae
herba
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CN101259197B (en
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施少斌
郑荣波
陈凤庭
廖广群
黄晓丹
秦春梅
廖晓嘉
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WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
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WANGLAOJI PHARMACEUTICAL CO Ltd GUANGZHOU CITY
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Abstract

The present invention provides a preparation method of Chinese medicine for curing cold. The Chinese medicine is made from honeysuckle, baikal skullcap root, herba schizonepetae, cape jasmine fruit, forsythia, radix scrophularise, batryticated silkworm, radix rehmanniae, blackberry lily, ballonflower, mint, cicada slough, radix sileris and liquorice. The Chinese medicine provided by the present invention adopts the ultrasonic extraction of the materials of honeysuckle, baikal skullcap root, herba schizonepetae, cape jasmine fruit, forsythia, radix scrophularise, batryticated silkworm, radix rehmanniae, blackberry lily, ballonflower, mint, cicada slough, radix sileris and liquorice, and then the preparation is obtained.

Description

A kind of pharmaceutical composition for the treatment of flu and preparation method thereof
Technical field
The present invention relates to field of medicaments, particularly relate to a kind of pharmaceutical composition for the treatment of flu and preparation method thereof.
Background technology
Flu is a kind of commonly encountered diseases and frequently-occurring disease, and especially at spring, winter sickness rate height, its symptom is nasal obstruction, watery nasal discharge, headache, cough, heating etc., makes us feeling very uncomfortable, can have influence on normal life when serious.At present commercially available Coritab is numerous in variety, mostly is compound preparation, and compound preparation mainly is to utilize between the various medicines to have synergism, brings into play the advantage of each medicine, reaches the purpose of symptomatic treatment.Gram sense sore-throat relieving oral liquid is the preparation that a kind of treatment commonly used is caught a cold, this pharmaceutical preparation is by Radix Scutellariae, Flos Lonicerae, Herba Schizonepetae, Fructus Gardeniae (stir-fry), Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens), Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Herba Menthae, Periostracum Cicadae, Radix Saposhnikoviae, Radix Glycyrrhizae totally 14 flavor medical materials is formed, but in the preparation technology of gram sense sore-throat relieving oral liquid, Radix Scutellariae adopts ethanol extraction separately, Flos Lonicerae, Radix Saposhnikoviae, Herba Schizonepetae, Herba Menthae four Chinese medicine material altogether adopts steam distillation to extract volatile oil, the Radix Scutellariae medicinal residues, 9 flavors such as the medicinal residues behind extraction such as the Flos Lonicerae volatile oil and all the other Fructus Gardeniaes, extracting in water, extracting solution adds the ethanol precipitate with ethanol.This technology is classical traditional decoction and alcohol sedimentation technique, but whole process energy consumption is big, and oil extracting rate is lower in the actual production, operates also more backwardly, and the high temperature extraction time is long, and the thermolability composition has certain destruction.
Summary of the invention
The purpose of this invention is to provide a kind of pharmaceutical composition for the treatment of flu.
Another object of the present invention provides a kind of preparation of drug combination method for the treatment of flu.
Pharmaceutical composition of the present invention is to be made by Flos Lonicerae, Radix Scutellariae, Herba Schizonepetae, Fructus Gardeniae, Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus, Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Herba Menthae, Periostracum Cicadae, Radix Saposhnikoviae, Radix Glycyrrhizae.
The present invention treats the cold medicine compositions, adopts following method preparation:
A. 36～145 parts of extracting honeysuckles, 36～145 parts of Radix Scutellariaes, 36～145 parts of Herba Schizonepetae, 36～145 parts of Fructus Gardeniaes, 36～145 parts of Fructus Forsythiaes, 36～145 parts of Radix Scrophulariaes, 22～85 parts of Bombyx Batryticatus, 55～215 parts of Radix Rehmanniae, 11～45 parts of Rhizoma Belamcandae, 22～85 parts of Radix Platycodoniss, 22～85 parts of Herba Menthaes, 22～85 parts of Periostracum Cicadaes, 22～85 parts of Radix Saposhnikoviaes, 11～45 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 1～10 times of water gaging, soak after 0.2～3 hour, adopt steam distillation 0.5～6 hour, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 10～35Mpa, 20～90 ℃ of extraction temperature, extraction time 10～240min collect extract; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 3～15 times of water gagings or 1～95% ethanol, ultrasonic power 300～5000w, 20～90 ℃, supersound extraction 1～4 time, each 10～120 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made present composition active component.
Preferred the present invention treats the cold medicine compositions, adopts following method preparation:
A. 53.54～86.70 parts of extracting honeysuckles, 53.54～86.70 parts of Radix Scutellariaes, 53.54～86.70 parts of Herba Schizonepetae, 53.54～86.70 parts of Fructus Gardeniaes, 53.54～86.70 parts of Fructus Forsythiaes, 53.54～86.70 parts of Radix Scrophulariaes, 33.57～51.55 parts of Bombyx Batryticatus, 86.27～129.81 parts of Radix Rehmanniae, 17.54～26.70 parts of Rhizoma Belamcandae, 33.57～51.55 parts of Radix Platycodoniss, 33.57～51.55 parts of Herba Menthaes, 33.57～51.55 parts of Radix Saposhnikoviaes, 33.57～51.55 parts of Periostracum Cicadaes, 17.54～26.70 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 2～8 times of water gagings, soak after 0.5～2 hour, adopt steam distillation 2～4 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 18～25Mpa, 40～60 ℃ of extraction temperature, extraction time 60～180min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2～15mm medical material granularity, 8～12 times of water gagings or 30～60% ethanol, ultrasonic power 1000～2500w, 40～70 ℃, supersound extraction 1～3 time, each 20～60 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made present composition active component.
Best the present invention treats the cold medicine compositions, adopts following method preparation:
A. 72 parts of extracting honeysuckles, 72 parts of Radix Scutellariaes, 72 parts of Herba Schizonepetae, 72 parts of Fructus Gardeniaes, 72 parts of Fructus Forsythiaes, 72 parts of Radix Scrophulariaes, 43 parts of Bombyx Batryticatus, 108 parts of Radix Rehmanniae, 22 parts of Rhizoma Belamcandae, 43 parts of Radix Platycodoniss, 43 parts of Herba Menthaes, 43 parts of Periostracum Cicadaes, 43 parts of Radix Saposhnikoviaes, 22 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, perhaps 8mm medical material granularity is measured 40% ethanol, ultrasonic power 1200w for 9 times; 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, and relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, made present composition active component.
Present composition effective ingredient and suitable adjuvant can be made any preparation on the pharmaceutics among the present invention; The active component of medicine of the present invention can add various conventional adjuvant required when preparing different dosage form, be prepared into any common formulations as disintegrating agent, lubricant, binding agent etc. with the method for Chinese medicinal of routine, as tablet, sugar coated tablet, film coated tablet, enteric coated tablet, powder, capsule, soft capsule, pill, electuary, oral liquid, granule, drop pill, micropill, intramuscular injection agent, instillation, unguentum, ointment, plaster etc.; The best is made tablet, drop pill, oral liquid, granule, capsule, soft capsule.
The present invention treats cold medicine preparation of compositions method, and step is as follows:
A. 36～145 parts of extracting honeysuckles, 36～145 parts of Radix Scutellariaes, 36～145 parts of Herba Schizonepetae, 36～145 parts of Fructus Gardeniaes, 36～145 parts of Fructus Forsythiaes, 36～145 parts of Radix Scrophulariaes, 22～85 parts of Bombyx Batryticatus, 55～215 parts of Radix Rehmanniae, 11～45 parts of Rhizoma Belamcandae, 22～85 parts of Radix Platycodoniss, 22～85 parts of Herba Menthaes, 22～85 parts of Periostracum Cicadaes, 22～85 parts of Radix Saposhnikoviaes, 11～45 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 1～10 times of water gaging, soak after 0.2～3 hour, adopt steam distillation 0.5～6 hour, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 10～35Mpa, 20～90 ℃ of extraction temperature, extraction time 10～240min collect extract; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 3～15 times of water gagings or 1～95% ethanol, ultrasonic power 300～5000w, 20～90 ℃, supersound extraction 1～4 time, each 10～120 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made preparation with suitable adjuvant.
Preferred the present invention treats cold medicine preparation of compositions method, and step is as follows:
A. 53.54～86.70 parts of extracting honeysuckles, 53.54～86.70 parts of Radix Scutellariaes, 53.54～86.70 parts of Herba Schizonepetae, 53.54～86.70 parts of Fructus Gardeniaes, 53.54～86.70 parts of Fructus Forsythiaes, 53.54～86.70 parts of Radix Scrophulariaes, 33.57～51.55 parts of Bombyx Batryticatus, 86.27～129.81 parts of Radix Rehmanniae, 17.54～26.70 parts of Rhizoma Belamcandae, 33.57～51.55 parts of Radix Platycodoniss, 33.57～51.55 parts of Herba Menthaes, 33.57～51.55 parts of Radix Saposhnikoviaes, 33.57～51.55 parts of Periostracum Cicadaes, 17.54～26.70 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 2～8 times of water gagings, soak after 0.5～2 hour, adopt steam distillation 2～4 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 18～25Mpa, 40～60 ℃ of extraction temperature, extraction time 60～180min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2～15mm medical material granularity, 8～12 times of water gagings or 30～60% ethanol, ultrasonic power 1000～2500w, 40～70 ℃, supersound extraction 1～3 time, each 20～60 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made preparation with suitable adjuvant.
Further preferred the present invention treats cold medicine preparation of compositions method, and step is as follows:
A. 72 parts of extracting honeysuckles, 72 parts of Radix Scutellariaes, 72 parts of Herba Schizonepetae, 72 parts of Fructus Gardeniaes, 72 parts of Fructus Forsythiaes, 72 parts of Radix Scrophulariaes, 43 parts of Bombyx Batryticatus, 108 parts of Radix Rehmanniae, 22 parts of Rhizoma Belamcandae, 43 parts of Radix Platycodoniss, 43 parts of Herba Menthaes, 43 parts of Periostracum Cicadaes, 43 parts of Radix Saposhnikoviaes, 22 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, perhaps 8mm medical material granularity is measured 40% ethanol, ultrasonic power 1200w for 9 times; 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, and relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, added the volatile oil or the CO of vapor distillations such as the Flos Lonicerae acquisition of an amount of cosolvent mixing of above-mentioned usefulness 2Supercritical extract is made preparation with suitable adjuvant.
Best the present invention treats cold medicine preparation of compositions method, among the step b, and the volatile oil or the CO that will obtain with the vapor distillations such as Flos Lonicerae of an amount of polysorbate-80 mixing 2Supercritical extract adds adjuvants such as an amount of correctives, adds water and adjusts total amount to 1000ml, stirs evenly, filter, and embedding, sterilization, promptly.
In pharmaceutical composition of the present invention or the preparation of drug combination method, Fructus Gardeniae is a Fructus Gardeniae (parched); Bombyx Batryticatus is the processed with Rhizoma Zingiberis Recens Bombyx Batryticatus.
In pharmaceutical composition of the present invention or the preparation of drug combination method, described ethanol is 1～95% ethanol.
In preferred pharmaceutical composition of the present invention or the preparation of drug combination method, described ethanol is 30～60% ethanol.
In further preferred pharmaceutical composition of the present invention or the preparation of drug combination method, described ethanol is 35～45% ethanol.
In the best pharmaceutical composition of the present invention or preparation of drug combination method, described ethanol is 40% ethanol.
The preparation method of medicinal tablet of the present invention is got present composition active component, mixes tabletting with tablet adjuvant commonly used.
The preparation method of preferred tablet of the present invention is got present composition active component, adds one or more combination of adjuvants such as starch, dextrin, lactose, PVP, carboxymethyl starch sodium, mix homogeneously, and tabletting, coating or coating not promptly get tablet.
The preparation method of drop pill of the present invention is got present composition active component, mixes with drop pill adjuvant commonly used, makes drop pill.
The preparation method of preferred drop pill of the present invention is got present composition active component, adds the one or any combination in polyethylene glycol 6000, Macrogol 4000, the polyethylene glycol 1500, heating dissolves, adopting a speed is 15～90/min, splashes in the condensed fluid, promptly gets drop pill.
The preparation method of further preferred drop pill of the present invention; get present composition active component; add the polyethylene glycol 6000 of 1～30 times of amount: Macrogol 4000=1～5: 1～4 mixture; heating dissolves; it is 25～75/min that speed is dripped in employing; splash into liquid Paraffin, or methyl-silicone oil or methyl-silicone oil: liquid Paraffin=1～4: in 1～3 the condensed fluid, promptly get drop pill.
The capsular preparation method of the present invention is got present composition effective ingredient, and one or more the combination adjuvant that adds adjuvants such as an amount of starch, dextrin, lactose, PVP, carboxymethyl starch sodium is granulated, and in the capsule for medicine of packing into, promptly gets capsule.
The preparation method of granule of the present invention is got present composition active component, granulates with granule adjuvant commonly used, with particle drying, promptly gets granule.
The preparation method of granule is dripped in preferred the present invention; get present composition active component; the combination of one or both of adding soluble starch, cyclodextrin adjuvant; the amount of used adjuvant is 0.4～3 times of present composition effective ingredient weight; mix homogeneously, adds water and granulates, and consumption is 10～30% of medicated powder and a supplementary product consumption; with particle drying, promptly gets granule.
Preparation of soft capsule method of the present invention is got present composition active component, makes soft capsule according to the conventional preparation method of soft capsule.
The preparation method of oral liquid of the present invention is got present composition effective ingredient, adds an amount of correctives, adds water and adjusts total amount to 1000ml, stirs evenly, filter, and embedding, sterilization, promptly.
The preparation method of best oral liquid of the present invention:
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae (parched) etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, added an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
More than form when producing and to increase or to reduce according to corresponding ratio, as large-scale production can be unit with kilogram or with the ton, small-scale production can be unit with the gram also, and weight can increase or reduce, but the crude drug material weight proportion constant rate between each composition.
The present invention be on basis, obtain the former Study on extraction process of gram sense sore-throat relieving oral liquid, in order to understand the pharmacology index of biological activity evaluation of the pharmaceutical composition that medicine material medicine extraction process experimental study of the present invention and above-mentioned different extraction process obtain better, the effect of the pharmaceutical composition of the present invention that different extraction processes obtain is described.
Test example one 13 flavors (or 14 flavors) medical material is used uniform Design and is carried out ultrasonic extraction process research
Among the gram sense sore-throat relieving oral liquid preparation technology, Radix Scutellariae medicinal residues, Flos Lonicerae etc. are extracted 9 flavors such as medicinal residues and all the other Fructus Gardeniaes behind the extract, adopt decocting to boil in former technology 2 times, each 2 hours, merge and concentrate behind the decocting liquid alcohol again and be sink to and contain alcohol and measure 60%.This technology is classical traditional decoction and alcohol sedimentation technique, but whole process energy consumption is big, and the high temperature extraction time is long, and the thermolability composition has certain destruction.In the present invention, consider to adopt ultrasonic extraction to substitute former decoction and alcohol sedimentation technique, determine in the research of full side's ultrasonic extraction process, propose three schemes:
In the present invention program's 1,2,3 researchs, all, amount to the 799g research experiment according to gram sense sore-throat relieving oral liquid prescription extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g.
Scheme 1:9 flavor medicine (Radix Scutellariae is the Radix Scutellariae medicinal residues after the alcohol extraction) can be considered the water supersound extraction, and Radix Scutellariae is constant by former technology.(i.e. 14 flavor crude drug, Radix Scutellariae extracts by the former extracting method of gram sense sore-throat relieving oral liquid with ethanol earlier; Flos Lonicerae, Herba Schizonepetae, Periostracum Cicadae, Radix Saposhnikoviae supercritical extraction (SFE) will be equivalent to the SFE medicinal residues+497g medical material of 230g medical material+be equivalent to the Radix Scutellariae medicinal residues of 72g Radix Scutellariae then, adopt the water supersound extraction.)
Scheme 2:10 flavor medicine (comprising Radix Scutellariae) adopts the water supersound extraction, can simplify technology, should be one of desirable technology.(i.e. 14 flavor crude drug, Flos Lonicerae, Herba Schizonepetae, Periostracum Cicadae, Radix Saposhnikoviae supercritical extraction (SFE) then with the SFE medicinal residues+569g medical material of 230g medical material, adopt the water supersound extraction.)
Scheme 3:14 flavor medicine (comprising Radix Scutellariae) adopts certain density ethanol ultrasonic extraction, can simplify technology, should be one of desirable technology.(that is: 14 flavor crude drug, Flos Lonicerae, Herba Schizonepetae, Periostracum Cicadae, Radix Saposhnikoviae supercritical extraction (SFE) will be equivalent to the SFE medicinal residues+residue 569g medical material of 230g medical material then, employing Different concentrations of alcohol supersound extraction.)
Above scheme 1 and 3 two schemes are arranged uniform Design simultaneously, and the optimization technology that scheme 2 can adopt scheme 1 to screen is extracted, and determines the optimised process of above scheme at last in conjunction with pharmacological screening.
Uniform Design (Uniform design) is a kind of test design method from the uniform Design angle that the calm and peaceful Wang Yuan of the famous mathematician Fang Kai of China proposes, compare with the orthogonal design of " neatly comparable ", the characteristics of uniform Design are in trial stretch fully " homodisperse, " and give up " neatly comparable ", each level of related factors is dispersed in the scope of experiment, reduce experiment number, result of the test adopts regression analysis more.The uniform Design method can be used as the screening study means, is used for the Study on Modernization of Chinese medicine, study accordingly in conjunction with relevant pharmacodynamics model screening with the uniform Design method, and be a favourable instrument of new Chinese medicine exploitation.Because the factor that this research needs to investigate reaches 6～7, adopt uniform Design can reduce experiment number, the rule in the least possible test number (TN) between announcement factor and index also is a kind of application to this mathematical statistics method of uniform Design.
1.1 instrument and reagent
Instrument
Waters 600 high performance liquid chromatographs (600 quaternary gradient pumps, 2996 DAD detectors, 717 automatic samplers, column oven); The Empower work station; Mettler AE 240 type electronic balances (100,000/, Switzerland Mettler Toledo Inc.).
SCQ-2400 type ultrasonic cleaner (120L, double frequency 25KHZ, 40KHZ, power 2400W):
Sound Pu, Shanghai ultrasonic equipment factory produces.
Crude drug source and lot number:
Figure A20071007976100121
Above medical material provides by Wanglaoji Pharmaceutical Co., Ltd., Guangzhou City.
Gram sense sore-throat relieving oral liquid, supersound extraction sample by former explained hereafter: self-control.
1.2 experiment content and result
1.2.1 produce 4 batch samples by former technology lab scale, set up standard diagram
Press the gram sense sore-throat relieving oral liquid of former explained hereafter respectively, prepare 4 parts of small samples, lot number is respectively 072601A, 072601B, 072602A, 072602B, above lab scale sample is carried out baicalin, jasminoidin HPLC analysis, wide spectrum chromatography, total solids test, deal with data, calculating the results are shown in Table 1 respectively.The HPLC chromatogram collection of illustrative plates unanimity of 4 batch samples, similarity result of calculation generate standard control collection of illustrative plates (common pattern) and see Fig. 1, with this standard diagram as supersound extraction research usefulness all greater than 0.99.
Table 1: the measurement result of lab scale sample
Figure A20071007976100122
1.2.213 flavor medical material (comprising the Radix Scutellariae medicinal residues) water ultrasonic extraction process research (scheme 1)
13 flavor medical materials (comprising the Radix Scutellariae medicinal residues) refer to: 4 flavor SFE medicinal residues such as Flos Lonicerae, Fructus Gardeniae (stir-fry), Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens), Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Periostracum Cicadae, Radix Glycyrrhizae.
Test method: in the prescription ratio, extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g amount to 799g.Wherein Radix Scutellariae is used earlier 65% alcohol reflux, Flos Lonicerae, Herba Schizonepetae, Periostracum Cicadae, Radix Saposhnikoviae supercritical extraction (SFE), then will (230gSFE medicinal residues+497g medical material+be equivalent to the Radix Scutellariae medicinal residues of 72g Radix Scutellariae), with medical material granularity, ultrasonic temperature, ultrasonic power, ultrasonic time, solvent load, extraction time is the investigation factor, select varying level, with total solid yield, jasminoidin content, content of baicalin, wide spectrum chromatogram similarity totally four as evaluation index, by uniform Design, adopt six factor table U 10(10 6) test, the gentle factor of test water sees Table 2, and result of the test sees Table 3, and as standard diagram, collection of illustrative plates is seen Fig. 2 with small sample in the phosphoramidite chemical evaluation.
Table 2: factor level table
Figure A20071007976100131
Figure A20071007976100141
1.2.3 full side 14 flavor medical materials adopt Different concentrations of alcohol ultrasonic extraction process research (scheme 3)
14 flavor medical materials comprise: 4 flavor SFE medicinal residues such as Flos Lonicerae, Radix Scutellariae, Fructus Gardeniae (stir-fry), Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens), Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Periostracum Cicadae, Radix Glycyrrhizae.
The research is wished the Radix Scutellariae reflux, extract, in the former technology is extracted merging with other 13 flavor water extract-alcohol precipitation, adopts the technology of ethanol ultrasonic extraction together, one of desirable technology that this imagines for us.
Method: in the prescription ratio, take by weighing 799g (230g medicinal residues+569 medical materials) at every turn, with medical material granularity, ultrasonic temperature, ultrasonic power, ultrasonic time, ethanol consumption, concentration of alcohol, extraction time is the investigation factor, select varying level, with total solid, baicalin, wide spectrum chromatogram as evaluation index, by uniform Design, adopt seven factor table U 10(10 7) test, the gentle factor of test water sees Table 4, and result of the test sees Table 5, and phosphoramidite chemical is estimated collection of illustrative plates and is seen Fig. 3.
Table 4: factor level table
Figure A20071007976100151
2 preliminary definite four optimization process routes
Adopt SAS software, respectively to the total solid yield of above two uniform Design, jasminoidin content, content of baicalin, wide spectrum chromatogram similarity the result of totally 4 indexs carry out variance analysis, multiple comparisons and regression analysis, preliminary definite following four process routes, as follows:
Technology 1:A 3B 3C 2D 3E 9F 2Technology 2:A 3B 3C 4D 3E 9F 2
Technology 3:A 3B 3C 4D 3E 9F 2Technology 4:A 2B 4C 3D 3E 7F 5G 2
It below only is the optimization process route that comes out by chemical analysis means Preliminary screening, in next step test, the method that chemistry is estimated and the pharmacologically active index screening combines will be adopted, above optimization technology is carried out demonstration test and pharmacodynamics screening, thereby further estimate the feasibility and the suitability of ultrasonic extraction process, could determine best ultrasonic extraction process like this.
2.1 new technology is carried out the chemical constituent evaluation
Take by weighing medical material in the prescription ratio, respectively according to aforementioned optimization technology, 3 parts of operation repetitives, each supersound extraction liquid sample is taken a sample respectively, dilution, be made into the need testing solution that is equivalent to contain medical material 0.04g/ml, measure the total solid yield respectively, jasminoidin content, content of baicalin, the wide spectrum chromatography, the common pattern of setting up with former technology lab scale sample carries out similarity calculating as standard diagram, simultaneously four confirmatory experiment and former technologies of optimizing process route are compared research, analysis result sees Table 6 ultrasonic demonstration test results and gathers, table 7 Product Validation result of the test gathers, and chromatogram is seen Fig. 4.
Table 6: ultrasonic demonstration test result gathers
Figure A20071007976100171
Table 7 Product Validation result of the test gathers
Analysis result is (by name with reference to the collection of illustrative plates file: former technology sample 072601B. cdf)
Figure A20071007976100181
3. result and discussion
1. parallel 3 parts respectively of above optimization technologies, the result shows, good reproducibility, stable operation, simple and feasible.
2. with standard diagram (common pattern is compared), intuitively as seen, the whole looks of the collection of illustrative plates of 14 samples are relatively more consistent, similarity result of calculation is taken jasminoidin, two indexs of content of baicalin into consideration all greater than 0.90, and the result shows, two index component contents of some supersound extraction are higher, jasminoidin content particularly, ultrasonic extraction generally is higher than former technology water extract-alcohol precipitation, and content of baicalin is also basic to be more or less the same with former technology.Above chemical analysis evaluation result shows, supersound extraction new technology operation feasible, and have and simplify technology, obviously shorten superiority such as extraction time.
The research of pharmaceutical composition of the present invention (pharmacology) index of biological activity of test example two different extraction process preparations
The purpose of this test is that the pharmacology index of biological activity of the gram sense sore-throat relieving oral liquid that adopts different extraction process preparations is studied, to be used for determining that good effect and extraction process simply restrain the preparation technology of sense sore-throat relieving oral liquid.
1. (pharmacology) index of biological activity determines
In conjunction with the function of gram sense sore-throat relieving oral liquid " wind-dispelling heat-dissipating, resolving toxin and disinhibiting the throat " cure mainly with and the treatment indication, determine to adopt following pharmacology index to carry out the pharmacodynamics evaluation: (1) suppresses resisiting influenza virus experiment in inflammatory swelling test, (3) body to influence, (2) that e. coli lipopolysaccharide causes fever in rabbits
2. the design of sample sets
(1) the gram sense sore-throat relieving oral liquid of technology 1 preparation according to the disclosed gram sense of pharmacopeia sore-throat relieving oral liquor medicine standard fabrication, is called for short technology 1.
(2) the gram sense sore-throat relieving oral liquid of technology 2 preparation: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SD volatile oil+Radix Scutellariae 65% ethanol extract+(medicinal residues behind the SD+Radix Scutellariae medicinal residues+residue 9 flavor medical materials) decocting boils extract behind the 60% ethanol precipitate with ethanol, makes gram and feels the sore-throat relieving oral liquid.Specifically, be called for short technology 2 according to embodiment 22 preparations.
(3) pharmaceutical composition of technology 3 preparations: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SFE-CO 2Extract+Radix Scutellariae 65% ethanol extract+(SFE-CO 2Back medicinal residues+Radix Scutellariae medicinal residues+residue 9 flavor medical materials) mix the back decocting and boil extract behind the 60% ethanol precipitate with ethanol, make gram sense sore-throat relieving oral liquid.Specifically, be called for short technology 3 according to embodiment 23 preparations.
(4) pharmaceutical composition of technology 4 preparations: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SFE-CO 2Extract+Radix Scutellariae 65% ethanol extract+(SFE-CO 2Back medicinal residues+Radix Scutellariae medicinal residues+residue 9 flavor medical materials) mix back water sonicated extract, make gram sense sore-throat relieving oral liquid.According to embodiment 24 preparations, be called for short technology 4.
(5) pharmaceutical composition of technology 5 preparations: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SD volatile oil+Radix Scutellariae 65% ethanol extract+(medicinal residues behind the SD+Radix Scutellariae medicinal residues+residue 9 flavor medical materials) mix back water sonicated extract, make gram sense sore-throat relieving oral liquid.According to embodiment 25 preparations, be called for short technology 5.
(6) pharmaceutical composition of technology 6 preparations: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SFE-CO 2Extract+(SFE-CO 2Medicinal residues+residue 10 flavor medical materials comprise radix scutellariae medicinal materials) mix back water sonicated extract, make gram sense sore-throat relieving oral liquid.Specifically, be called for short technology 6 according to embodiment 10 preparations.
(7) pharmaceutical composition of technology 7 preparations: (Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae four flavors) SFE-CO 2Extract+(SFE-CO 2Medicinal residues+residue 10 flavor medical materials comprise Radix Scutellariae) mix the pure sonicated extract in back, make gram sense sore-throat relieving oral liquid.Specifically, be called for short technology 7 according to embodiment 11 preparations.
3 different process gram sense sore-throat relieving oral liquid causes the analgesic experiment of fever in rabbits to escherichia coli endotoxin (LPS)
3.1 test material
3.1.1 be subjected to the reagent thing: the gram sense sore-throat relieving oral liquid of seven Different Preparation is provided by Wanglaoji Pharmaceutical Co., Ltd., Guangzhou City.1.2g crude drug/ml, lot number 060911.
The dosage design: the clinical consumption of people is 48g crude drug/60kg/d, and two dosage groups of 10g, 5g crude drug/kg/d (being respectively 12.5 times and 6.25 times of amounts of the clinical consumption of people) are adopted in the analgesic experiment of rabbit respectively.Gastric infusion.
3.1.2 positive control drug: Aspirin Enteric-coated Tablets, Hefei join pharmaceutical Co. Ltd for a long time to be produced, and the used dosage of rabbit is (100mg/kg/d) gastric infusion during experiment.
3.1.3 animal: white big ear rabbit, male and female half and half.Body weight is 15 ± 2.5kg, and moving plant provides by Haidian, Beijing Tian Wang experiment, licence numbering: SCXK (capital) 2006-006.
3.1.4 instrument and reagent:
Endotoxin: Lipopolysaccharides Sigma L2800
Thermometer: WMY-01 digital thermometer Huachen Medical Meters Co Ltd, Shanghai produces.
3.2 method and result:
3.2.1 the selection of rabbit: select healthy anosis rabbit, survey body temperature every day once, continuous three days, make rabbit adapt to survey the body temperature operation, select 38.6-39.8 ℃ of body temperature, and the body temperature fluctuation 0.3 ℃ with interior person, uniform distribution is in each group of experiment at random.
3.2.2 experimental technique: get 160 of the qualified rabbit of body temperature, be divided into 16 groups by the basal body temperature stratified random, 10 every group, be respectively matched group, aspirin matched group, each high and low dose group of gram sense sore-throat relieving technology 1-7, each organizes gastric infusion, and matched group is given with the volume distilled water.After administration 1 hour, every rabbit is all from auricular vein injection escherichia coli endotoxin 250ng/kg (1ml/kg), endotoxin is attacked and was respectively organized gastric infusion in back 1 hour, after administration, respectively surveyed body temperature once in 1,2,3,4 hour, with the basal body temperature is radix, calculate the variation of each minute rabbit body temperature, carry out statistical procedures with difference T check between group.Calculate suppression ratio by following formula:
3.2.3 experimental result: see table 7 for details.
By table 7 as seen, body temperature can raise 1.59-1.88 ℃ behind the model control group endotoxin pyrogenic, after being administered once, except that technology 3 high low dosages there is no the tangible refrigeration function, all the other each technologies all had tangible refrigeration function in 1 hour behind medicine, and the refrigeration function of high dose group is better than low dose group.From separating on the thermal effect, technology 2 high dose group effects are the strongest, tangible refrigeration function all arranged in 1-4 hour after the administration, with matched group significant difference arranged relatively.It is also fine that technology 4,5,6,7 is separated thermal effect, behind the medicine 1 hour the effect the strongest, with matched group significant difference is arranged relatively.
Figure A20071007976100221
The comparison of 4 different process gram sense sore-throat relieving xylol induced mice ear swelling antiinflammatory action
4.1 experiment material
4.1.1 supply the reagent thing: the gram sense sore-throat relieving oral liquid of seven Different Preparation provides intermediate by Wanglaoji Pharmaceutical Co., Ltd., Guangzhou City, the unified preparation in this chamber.1.2g crude drug/ml, lot number 060911.
4.1.2 positive drug: hydrocortisone injection, produce lot number by Tianjin gold credit aminoacid company limited: 0501201
4.1.3 animal: kunming mice, the cleaning level, body weight 21～23g, male and female half and half are provided by Test Animal Centre, Academy of Military Medical Sciences, P.L.A, quality certification SCXK-(army) 2002-001.
4.2 method and result
4.2.1 experimental technique:
Get 224 of mices, by body weight stratified random grouping, experimentize in two batches by sex, every batch establish contrast, technology 1 is high and low, technology 2 is high and low, technology 3 is high and low, and technology 4 is high and low, and technology 5 is high and low, technology 6 is high and low, totally 16 groups of the high and low and positive drug of technology 7,14 every group, male and female half and half.Wherein high dose group is 24g crude drug/kg, is equivalent to 30 times (adult presses 60kg and calculates) of clinical adult's consumption; Low dose group is 12g crude drug/kg, is equivalent to 15 times of clinical people's consumption, by the pre-administration of statement dosage 3 days, every day 1 time.Cause the scorching same day, administration is 1 time again, and 1h behind the medicine, auris dextra only are coated with dimethylbenzene 80ul/, put to death mice after 15 minutes, get left and right auricle respectively with the 9mm card punch, and scales/electronic balance weighing calculates the swelling rate, checks through t-with Stata software and carries out statistical analysis.
4.2.2 result: see table 8 for details.
The result shows that technology 1 to technology 7 all has significant anti-acute inflammation effect, by the ordering of inhibition degree is:
High dose group: technology 6＞technology 3＞technology 7＞technology 4＞technology 5＞technology 2＞technology 1
Low dose group: technology 6＞technology 7＞technology 5＞technology 4＞technology 3＞technology 2＞technology 1
High low dosage combination and ordering are: technology 6＞technology 7＞technology 3＞technology 4＞technology 5＞technology 2＞technology 1
Table 8: the inhibitory action of different process gram sense xylol induced mice ear swelling
Figure A20071007976100231
Figure A20071007976100241
Annotate: compare with the blank group, the t-check, *: p＜0.05; *: p＜0.01; * *: p＜0.001;
4.3. conclusion: comparatively speaking, the antiphlogistic effects of technology 6 is best, and the antiphlogistic effects of technology 1 is relatively poor.But in general, each technology all has the obvious anti-inflammatory and anti effect, should take all factors into consideration various factorss such as equipment in the technology, cost, environmental protection during evaluation, needn't stick to the absolute figure in this experiment.
The comparative study of 5 different process gram sense sore-throat relieving oral liquid antivirus test
5.1 test material
5.1.1 be subjected to the reagent thing: gram sense sore-throat relieving oral liquid different process 1-7 number is provided by China Academy of Sciences Guanganmen Hospital.Contain 1.2g crude drug/ml medicinal liquid.Lot number: 060911.
The dosage design: the clinical consumption of people is 48g crude drug/60kg/d, and two dosage groups of 24g, 12g crude drug/kg/d (being respectively 30 times and 15 times of amounts of the clinical consumption of people) are adopted in experiment respectively in the mice body.Gastric infusion.
5.1.2 positive control drug: virazole, Bin Hu pharmaceutical factory in Hubei produces, lot number: 970512, the used dosage of mice is (0.07g/kg/d) gastric infusion during experiment.
5.1.3 animal: CD-1 (ICR) SPF/VAF kind mice, the male and female dual-purpose.Body weight is 14 ± 1g, and dimension tonneau China laboratory animal technology Co., Ltd provides by Beijing, licence numbering: SCXK (capital) 2002-0003.5.1.4 virus: influenza virus A-prime Mus lung adapted strain (FM1), available from Inst. of Viruses, China Preventive Medicine Science Academy, to go down to posterity by my research department ,-70 ℃ of preservations are standby.
5.2 method and result: the inhibitory action to mice influenza virus property pneumonia is got 170 of mices, is divided into 17 groups at random by body weight.Be respectively gram sense sore-throat relieving technology 1-7 high and low dose group; Virazole positive controls, viral infection matched group and normal control group.Except that the normal control group, mice is slightly anaesthetized with ether, with 15 LD 50Influenza virus drop nose infects, every 0.05ml.Infect and to begin gastric infusion the previous day, every day 1 time, each 0.2ml/10g body weight, continuous 5 days, matched group under equal conditions distilled water was irritated stomach.Dissected after taking by weighing the mice body weight on the 6th day, win full lung and weigh, calculate the lung exponential quantity, and obtain lung index suppression ratio.Lung index=[heavy (the g)/body weight (g) of lung] * 100
Figure A20071007976100251
Figure A20071007976100252
Statistical procedures is carried out in the t check between employing group as a result.
5.2.2 experimental result sees Table 9.
Table 9: gram sense sore-throat relieving is to the inhibitory action of mice influenza virus property pneumonia
Figure A20071007976100253
Compare with the normal control group: compare with the virus control group ##P＜0.01: *P＜0.01
Compare with the virus control group: *P＜0.05
Table 9 is the result show: the lung exponential quantity of technology 4 high dose group is starkly lower than the virus control group in 7 samples, with the virus control group significant difference (P＜0.01) is arranged relatively.Show that gram sense sore-throat relieving technology 4 high doses have obvious inhibitory action to the mice pneumonia that influenza virus causes.
Result and discussion
The research thinking that chemistry is estimated and biological activity (pharmacology) index combines is adopted in this research, the screening ultrasonic extraction process.In chemical evaluation study, adopt similarity evaluation, baicalin, jasminoidin content and dried cream yield totally four indexs as evaluation means, arrange Uniform Design, with medical material granularity, ultrasonic temperature, ultrasonic power, ultrasonic time, solvent consumption, solvent concentration, extraction time is the investigation factor, adopts six factor table U respectively 10(10 6) arrange 13 flavor medical material water supersound extraction tests, reach seven factor table U 10(10 7) arrange 14 flavor medical material alcohol supersound extraction tests, optimize process route.On this basis, in conjunction with the function of gram sense sore-throat relieving oral liquid " wind-dispelling heat-dissipating, resolving toxin and disinhibiting the throat " cure mainly with and the treatment indication, influence, (2) of adopting (1) that e. coli lipopolysaccharide is caused fever in rabbits suppress in inflammatory swelling test, (3) body the resisiting influenza virus experiment totally three pharmacology's indexs carry out the pharmacodynamics evaluation, finally determine ultrasound condition of the present invention.The result shows, adopts above ultrasonic extraction process practical, and is simple to operate stable, can be used as the novel technology for extracting of gram sense sore-throat relieving oral liquid.
Description of drawings
Provide the HPLC finger printing below, be intended to further specify the present invention, but the present invention is not construed as limiting.
Fig. 1: the standard control collection of illustrative plates (common pattern) that is used for supersound extraction research
Fig. 2: 13 flavor medical material (comprising the Radix Scutellariae medicinal residues) water supersound extraction Uniform Design chromatograms
Fig. 3: 14 flavor medical material alcohol supersound extraction Uniform Design chromatograms
Fig. 4: optimize process route demonstration test and former technology comparison colours spectrogram for four
Specific embodiment
Further set forth the preparation method of medicine of the present invention by the following examples.
Embodiment 1
A. get prescription one: Flos Lonicerae 36g, Radix Scutellariae 36g, Herba Schizonepetae 36g, Fructus Gardeniae 36g, Fructus Forsythiae 36g, Radix Scrophulariae 36g, Bombyx Batryticatus 22g, Radix Rehmanniae 55g, Rhizoma Belamcandae 11g, Radix Platycodonis 22g, Herba Menthae 22g, Periostracum Cicadae 22g, Radix Saposhnikoviae 22g, Radix Glycyrrhizae 11g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 35Mpa, 90 ℃ of extraction temperature, extraction time 240min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 15 times of water gagings, ultrasonic power 5000w, 90 ℃, supersound extraction 4 times, each 120 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds extracts such as above-mentioned Flos Lonicerae, make present composition active component, add dextrin, mix homogeneously, adding concentration is 80% alcohol granulation, with particle drying, adds magnesium stearate, mix homogeneously, tabletting promptly gets tablet.
Embodiment 2
A. get prescription two: Flos Lonicerae 145g, Radix Scutellariae 145g, Herba Schizonepetae 145g, Fructus Gardeniae (parched) 145g, Fructus Forsythiae 145g, Radix Scrophulariae 145g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 85g, Radix Rehmanniae 215g, Rhizoma Belamcandae 45g, Radix Platycodonis 85g, Herba Menthae 85g, Periostracum Cicadae 85g, Radix Saposhnikoviae 85g, Radix Glycyrrhizae 45g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 10Mpa, 20 ℃ of extraction temperature, extraction time 10min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae (parched), measure ethanol, ultrasonic power 300w, 20 ℃ for 3 times, supersound extraction 1 time was extracted 120 minutes, filtered, filtrate simmer down to clear paste adds extracts such as above-mentioned Flos Lonicerae, makes present composition active component, add starch, lactose, mix homogeneously, adding concentration is 5% alcohol granulation, with particle drying, add silica gel, mix homogeneously, tabletting, coating promptly gets tablet.
Embodiment 3
A. get prescription three: Flos Lonicerae 145g, Radix Scutellariae 45g, Herba Schizonepetae 36g, Fructus Gardeniae 36g, Fructus Forsythiae 145g, Radix Scrophulariae 36g, Bombyx Batryticatus 22g, Radix Rehmanniae 55g, Rhizoma Belamcandae 11g, Radix Platycodonis 85g, Herba Menthae 85g, Periostracum Cicadae 22g, Radix Saposhnikoviae 85g, Radix Glycyrrhizae 45g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 30Mpa, 80 ℃ of extraction temperature, extraction time 200min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 14 times of water gagings, ultrasonic power 4500w, 85 ℃, supersound extraction 3 times, each 100 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste, add extracts such as above-mentioned Flos Lonicerae, make present composition active component, add PVP, carboxymethyl starch sodium, mix homogeneously, adding concentration is 75% alcohol granulation, with particle drying, adds magnesium stearate, Polyethylene Glycol, mix homogeneously, tabletting, coating or coating not promptly get tablet.
Embodiment 4
A. get prescription four: Flos Lonicerae 86.70g, Radix Scutellariae 86.70g, Herba Schizonepetae 86.70g, Fructus Gardeniae (stir-fry) 86.70g, Fructus Forsythiae 86.70g, Radix Scrophulariae 86.70g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 51.55g, Radix Rehmanniae 129.81g, Rhizoma Belamcandae 26.70g, Radix Platycodonis 51.55g, Herba Menthae 51.55g, Radix Saposhnikoviae 51.55g, Periostracum Cicadae 51.55g, Radix Glycyrrhizae 26.70g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 25Mpa, 60 ℃ of extraction temperature, extraction time 180min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 15mm medical material granularity, 12 times of water gagings, ultrasonic power 2500w, 70 ℃, supersound extraction 3 times, each 60 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds carboxymethyl starch sodium, mix homogeneously is granulated, with particle drying, add extracts such as above-mentioned Flos Lonicerae, add silica gel, mix homogeneously, mix homogeneously in the capsule for medicine of packing into, promptly gets capsule.
Embodiment 5
A. get prescription five: Flos Lonicerae 53.54g, Radix Scutellariae 53.54g, Herba Schizonepetae 53.54g, Fructus Gardeniae (stir-fry) 53.54g, Fructus Forsythiae 53.54g, Radix Scrophulariae 53.54g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 33.57g, Radix Rehmanniae 86.27g, Rhizoma Belamcandae 17.54g, Radix Platycodonis 33.57g, Herba Menthae 33.57g, Radix Saposhnikoviae 33.57g, Periostracum Cicadae 33.57g, Radix Glycyrrhizae 17.54g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 18Mpa, 40 ℃ of extraction temperature, extraction time 60min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2mm medical material granularity, 8 times of water gagings or ethanol, ultrasonic power 1000w, 40 ℃, supersound extraction 2 times, each 20 minutes, merge extractive liquid, filters filtrate simmer down to clear paste, the extracts such as Flos Lonicerae that make are added the Tween 80 mix homogeneously, it is an amount of to add water, with mulser emulsifying 10min, gets extract Emulsion; Get above-mentioned clear paste, mixing with homogenize machine homogenize 30min, adds extract Emulsion mix homogeneously again, with homogenize machine homogenize 30min, make clear paste 12.61%, extract 0.14%, substrate 25%, glycerol 1.25%, water 5%, the soft capsule content of tween 56%, described substrate is vegetable oil, Polyethylene Glycol or the Tween 80 of equivalent; Get the foregoing thing and suppress soft capsule with the rotation rolling capsule machine.
Embodiment 6
A. get prescription six: Flos Lonicerae 86.70g, Radix Scutellariae 53.54g, Herba Schizonepetae 86.70g, Fructus Gardeniae (stir-fry) 53.54g, Fructus Forsythiae 86.70g, Radix Scrophulariae 53.54g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 51.55g, Radix Rehmanniae 86.27g, Rhizoma Belamcandae 26.70g, Radix Platycodonis 33.57g, Herba Menthae 51.55g, Radix Saposhnikoviae 33.57g, Periostracum Cicadae 51.55g, Radix Glycyrrhizae 17.54g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 26Mpa, 65 ℃ of extraction temperature, extraction time 130min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 14mm medical material granularity is measured ethanol for 12 times, ultrasonic power 2400w, 65 ℃, supersound extraction 2 times, each 55 minutes, merge extractive liquid, filters filtrate simmer down to clear paste, extracts such as Flos Lonicerae add the Tween 80 mix homogeneously, it is an amount of to add water, with mulser emulsifying 10min, gets extract Emulsion; Get glycerol, substrate, clear paste of the present invention, mixing with homogenize machine homogenize 30min, adds extract Emulsion mix homogeneously again, with homogenize machine homogenize 30min, make medicated powder 12.61%, extract 0.14%, substrate 25%, glycerol 1.25%, water 5%, the soft capsule content of tween 56%, described substrate is Polyethylene Glycol; Get the foregoing thing and suppress soft capsule with the rotation rolling capsule machine.
Embodiment 7
A. get prescription seven: Flos Lonicerae 75g, Radix Scutellariae 75g, Herba Schizonepetae 75g, Fructus Gardeniae 75g, Fructus Forsythiae 75g, Radix Scrophulariae 75g, Bombyx Batryticatus 47g, Radix Rehmanniae 115g, Rhizoma Belamcandae 25g, Radix Platycodonis 47g, Herba Menthae 47g, Periostracum Cicadae 47g, Radix Saposhnikoviae 47g, Radix Glycyrrhizae 24g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 18～25Mpa, 40～60 ℃ of extraction temperature, extraction time 60～180min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2～15mm medical material granularity, 8～12 times of water gagings or ethanol, ultrasonic power 1000～2500w, 40～70 ℃, supersound extraction 1～3 time, each 20～60 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds extracts such as above-mentioned Flos Lonicerae, adds cyclodextrin, the amount of used cyclodextrin is 2 times of clear paste weight, mix homogeneously adds water and granulates, and makes granule.
Embodiment 8
A. get prescription eight: Flos Lonicerae 69g, Radix Scutellariae 69g, Herba Schizonepetae 69g, Fructus Gardeniae 69g, Fructus Forsythiae 69g, Radix Scrophulariae 69g, Bombyx Batryticatus 40g, Radix Rehmanniae 100g, Rhizoma Belamcandae 20g, Radix Platycodonis 40g, Herba Menthae 40g, Periostracum Cicadae 40g, Radix Saposhnikoviae 40g, Radix Glycyrrhizae 20g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 20Mpa, 45 ℃ of extraction temperature, extraction time 80min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 6mm medical material granularity is measured 40% ethanol for 9 times, ultrasonic power 800w, 60 ℃, supersound extraction 2 times, each 50 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds extracts such as above-mentioned Flos Lonicerae, and the adding cane sugar powder is an amount of, mix homogeneously, granulate,, promptly get granule particle drying.
Embodiment 9
A. get prescription nine: Flos Lonicerae 75g, Radix Scutellariae 69g, Herba Schizonepetae 75g, Fructus Gardeniae 69g, Fructus Forsythiae 75g, Radix Scrophulariae 75g, Bombyx Batryticatus 40, Radix Rehmanniae 115g, Rhizoma Belamcandae 25g, Radix Platycodonis 40g, Herba Menthae 40g, Periostracum Cicadae 40g, Radix Saposhnikoviae 47g, Radix Glycyrrhizae 20g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, added soluble starch, mix homogeneously, water is granulated, and with particle drying, promptly gets granule.
The preparation of embodiment 10 technologies 6
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae (parched) etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of above-mentioned usefulness an amount of polysorbate-80 mixing, added an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
The preparation of embodiment 11 technologies 7
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae (parched) etc. 10 medical materials of distinguishing the flavor of, 8mm medical material granularity, 40% ethanol of 9 times of amounts, ultrasonic power 1200w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of above-mentioned usefulness an amount of polysorbate-80 mixing, added an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
Embodiment 12
A. extracting honeysuckle 145g, Radix Scutellariae 145g, Herba Schizonepetae 145g, Fructus Gardeniae 145g, Fructus Forsythiae 145g, Radix Scrophulariae 145g, Bombyx Batryticatus 85g, Radix Rehmanniae 215g, Rhizoma Belamcandae 45g, Radix Platycodonis 85g, Herba Menthae 85g, Periostracum Cicadae 85g, Radix Saposhnikoviae 85g, Radix Glycyrrhizae 45g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 18Mpa, 70 ℃ of extraction temperature, extraction time 50min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 12mm medical material granularity, 5 times of water gagings, ultrasonic power 1500w, 40 ℃, supersound extraction 2 times, each 40 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 40 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, mix homogeneously, add Macrogol 4000, heating dissolves, and adopting a speed is 80/min, splash in the condensed fluid, promptly get drop pill.
Embodiment 13
A. extracting honeysuckle 145g, Radix Scutellariae 45g, Herba Schizonepetae 36g, Fructus Gardeniae 36g, Fructus Forsythiae 145g, Radix Scrophulariae 36g, Bombyx Batryticatus 22g, Radix Rehmanniae 55g, Rhizoma Belamcandae 11g, Radix Platycodonis 85g, Herba Menthae 85g, Periostracum Cicadae 22g, Radix Saposhnikoviae 85g, Radix Glycyrrhizae 45g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 8Mpa, 80 ℃ of extraction temperature, extraction time 150min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 8mm medical material granularity, 15 times of amount 75% ethanol, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid,, filter, relative density was 1.10～1.21 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, made present composition active component.
C. get it filled powder I, medicated powder II, extract, mix homogeneously adds polyethylene glycol 1500, and heating dissolves, and adopts that to drip speed be 85/min, splashes in the condensed fluid, promptly gets drop pill.
Embodiment 14
A. extracting honeysuckle 86.70g, Radix Scutellariae 86.70g, Herba Schizonepetae 86.70g, Fructus Gardeniae (stir-fry) 86.70g, Fructus Forsythiae 86.70g, Radix Scrophulariae 86.70g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 51.55g, Radix Rehmanniae 129.81g, Rhizoma Belamcandae 26.70g, Radix Platycodonis 51.55g, Herba Menthae 51.55g, Radix Saposhnikoviae 51.55g, Periostracum Cicadae 51.55g, Radix Glycyrrhizae 26.70g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filters, and relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, the extracts such as Flos Lonicerae that add an amount of cosolvent mixing of above-mentioned usefulness, add PVP, mix homogeneously, adding concentration is 50% alcohol granulation, with particle drying, mix homogeneously in the capsule for medicine of packing into, promptly gets capsule.
Embodiment 15
A. extracting honeysuckle 53.54g, Radix Scutellariae 53.54g, Herba Schizonepetae 53.54g, Fructus Gardeniae (stir-fry) 53.54g, Fructus Forsythiae 53.54g, Radix Scrophulariae 53.54g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 33.57g, Radix Rehmanniae 86.27g, Rhizoma Belamcandae 17.54g, Radix Platycodonis 33.57g, Herba Menthae 33.57g, Radix Saposhnikoviae 33.57g, Periostracum Cicadae 33.57g, Radix Glycyrrhizae 17.54g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues and all the other Radix Scutellariaes after the 4 flavor medical material supercritical extraction such as Flos Lonicerae, 10 flavor medical materials such as Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid,, filter, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, the polyethylene glycol 6000 that adds 10 times of amounts of medicated powder: the polyethylene glycol 6000 and the Macrogol 4000 mixture of Macrogol 4000=5: 1, heating dissolves, and adopting a speed is 65～75/min, splashes into condensed fluid (liquid Paraffin, or methyl-silicone oil: liquid Paraffin=in 4: 1, promptly get drop pill.
Embodiment 16
A. extracting honeysuckle 86.70g, Radix Scutellariae 53.54g, Herba Schizonepetae 86.70g, Fructus Gardeniae (stir-fry) 53.54g, Fructus Forsythiae 86.70g, Radix Scrophulariae 53.54g, Bombyx Batryticatus (processed with Rhizoma Zingiberis Recens) 51.55g, Radix Rehmanniae 86.27g, Rhizoma Belamcandae 26.70g, Radix Platycodonis 33.57g, Herba Menthae 51.55g, Radix Saposhnikoviae 33.57g, Periostracum Cicadae 51.55g, Radix Glycyrrhizae 17.54g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid,, filter, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, made present composition active component.
C. get it filled powder I, medicated powder II, extract, mix homogeneously, the polyethylene glycol 6000 that adds 4 times of amounts of medicated powder: the polyethylene glycol 6000 and the Macrogol 4000 mixture of Macrogol 4000=1: 1, heating dissolves, adopting a speed is 45～55/min, splash into methyl-silicone oil: in the condensed fluid of liquid Paraffin=4: 1, promptly get drop pill.
Embodiment 17
A. extracting honeysuckle 75g, Radix Scutellariae 75g, Herba Schizonepetae 75g, Fructus Gardeniae 75g, Fructus Forsythiae 75g, Radix Scrophulariae 75g, Bombyx Batryticatus 47g, Radix Rehmanniae 115g, Rhizoma Belamcandae 25g, Radix Platycodonis 47g, Herba Menthae 47g, Periostracum Cicadae 47g, Radix Saposhnikoviae 47g, Radix Glycyrrhizae 24g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, the extracts such as Flos Lonicerae that add an amount of cosolvent mixing of above-mentioned usefulness add starch, mix homogeneously, adding concentration is 75% alcohol granulation, and consumption is 8% of medicated powder and a supplementary product consumption; With particle drying, add the magnesium stearate mix homogeneously, mix homogeneously in the capsule for medicine of packing into, promptly gets capsule.
Embodiment 18
A. extracting honeysuckle 69g, Radix Scutellariae 69g, Herba Schizonepetae 69g, Fructus Gardeniae 69g, Fructus Forsythiae 69g, Radix Scrophulariae 69g, Bombyx Batryticatus 40g, Radix Rehmanniae 100g, Rhizoma Belamcandae 20g, Radix Platycodonis 40g, Herba Menthae 40g, Periostracum Cicadae 40g, Radix Saposhnikoviae 40g, Radix Glycyrrhizae 20g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 10mm medical material granularity is measured 65% ethanol for 11 times, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filters, and relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, the extracts such as Flos Lonicerae that add an amount of cosolvent mixing of above-mentioned usefulness, add an amount of cane sugar powder,, make pill with honey refining ball.
Embodiment 19
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae (parched), 10mm medical material granularity is measured 65% ethanol for 11 times, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, added an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
Embodiment 20
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 20Mpa, 75 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae (parched), 10mm medical material granularity is measured 75% ethanol for 10 times, ultrasonic power 1600w, 70 ℃, supersound extraction 3 times, each 40 minutes, merge extractive liquid, filtered, relative density was 1.06～1.25 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, added an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
Embodiment 21
A. extracting honeysuckle 36g, Radix Scutellariae 36g, Herba Schizonepetae 36g, Fructus Gardeniae 36g, Fructus Forsythiae 36g, Radix Scrophulariae 36g, Bombyx Batryticatus 22g, Radix Rehmanniae 55g, Rhizoma Belamcandae 11g, Radix Platycodonis 22g, Herba Menthae 22g, Periostracum Cicadae 22g, Radix Saposhnikoviae 22g, Radix Glycyrrhizae 11g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 24Mpa, 60 ℃ of extraction temperature, extraction time 90min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 8mm medical material granularity, 7 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 35 minutes, merge extractive liquid, filtered, relative density was 1.06～1.31 when filtrate was concentrated into 60 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, mix homogeneously, add polyethylene glycol 6000, heating dissolves, and adopting a speed is 90/min, splash in the condensed fluid, promptly get drop pill.
The preparation of embodiment 22 technologies 2
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Radix Scutellariae adds 65% ethanol extraction 2 times, and each 2 hours, reclaim ethanol, add water and boil in right amount, filter filtrate for later use while hot; With medicinal residues behind the Radix Scutellariae ethanol extraction and Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae is extracted medicinal residues and the Fructus Gardeniae behind the volatile oil, Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus, Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Periostracum Cicadae, Radix Glycyrrhizae decocts with water 2 times, 2 hours for the first time, 1 hour for the second time, collecting decoction, filter, it is 1.06～1.11 (70 ℃) that filtrate is concentrated into relative density, adding ethanol makes pure content reach 60% and leaves standstill, filter, filtrate recycling ethanol and to be concentrated into relative density be 1.16～1.20 (70 ℃) adds volatile oil such as above-mentioned Flos Lonicerae (adding an amount of tween 80 mixing), aqueous solution after the distillation, Radix Scutellariae ethanol extract and stevioside 0.5g, sodium benzoate 3g, add water and adjust total amount to 1000ml, mixing filters embedding, sterilization, promptly.
The preparation of embodiment 23 technologies 3
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; Radix Scutellariae adds 65% ethanol extraction 2 times, and each 2 hours, reclaim ethanol, add water and boil in right amount, filter filtrate for later use while hot; With the medicinal residues behind the 4 flavor medical material supercritical extractions such as Flos Lonicerae and Radix Scutellariae medicinal residues and residue Fructus Gardeniae Fructus Gardeniae, Fructus Forsythiae, Radix Scrophulariae, Bombyx Batryticatus, Radix Rehmanniae, Rhizoma Belamcandae, Radix Platycodonis, Periostracum Cicadae, Radix Glycyrrhizae decocts with water 2 times, 2 hours for the first time, 1 hour for the second time, collecting decoction, filter, it is 1.06～1.11 (70 ℃) that filtrate is concentrated into relative density, adding ethanol makes pure content reach 60% and leaves standstill, filter, filtrate recycling ethanol and to be concentrated into relative density be 1.16～1.20 (70 ℃), add extracts such as above-mentioned Flos Lonicerae (adding an amount of tween 80 mixing), Radix Scutellariae ethanol extract and stevioside 0.5g, sodium benzoate 3g adds water and adjusts total amount to 1000ml, mixing, filter, embedding, sterilization, promptly.
The preparation of embodiment 24 technologies 4
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; Radix Scutellariae adds 65% ethanol extraction 2 times, and each 2 hours, reclaim ethanol, add water and boil in right amount, filter filtrate for later use while hot; With medicinal residues behind the 4 flavor medical material supercritical extractions such as Flos Lonicerae and 9 flavor medical materials such as Radix Scutellariae medicinal residues and residue Fructus Gardeniae Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, added the CO such as Flos Lonicerae of an amount of polysorbate-80 mixing 2Supercritical extract, the Radix Scutellariae ethanol extract adds adjuvants such as an amount of correctives, adds water and adjusts total amount to 1000ml, stirs evenly, filter, embedding, sterilization, promptly.
The preparation of embodiment 25 technologies 5
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae (parched) 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, processed with Rhizoma Zingiberis Recens Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Radix Scutellariae adds 65% ethanol extraction 2 times, and each 2 hours, reclaim ethanol, add water and boil in right amount, filter filtrate for later use while hot; With medicinal residues behind the 4 flavor medical material supercritical extractions such as Flos Lonicerae and 9 flavor medical materials such as Radix Scutellariae medicinal residues and residue Fructus Gardeniae Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, and the Radix Scutellariae ethanol extract adds adjuvants such as an amount of correctives, add water and adjust total amount, stir evenly, filter to 1000ml, embedding, sterilization, promptly.
Embodiment 26
A. extracting honeysuckle 40g, Radix Scutellariae 140g, Herba Schizonepetae 45g, Fructus Gardeniae 45g, Fructus Forsythiae 140g, Radix Scrophulariae 40g, Bombyx Batryticatus 25g, Radix Rehmanniae 60g, Rhizoma Belamcandae 15g, Radix Platycodonis 25g, Herba Menthae 25g, Periostracum Cicadae 25g, Radix Saposhnikoviae 25g, Radix Glycyrrhizae 25g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 10 times of water gagings, soak after 3 hours, adopt steam distillation 6 hours, collect volatile oil; With medicinal residues behind the 4 flavor medical material water vapour distillation such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 15 times of water gagings, ultrasonic power 300～5000w, 20～90 ℃, supersound extraction 4 times, each 10 minutes, merge extractive liquid, filters filtrate simmer down to clear paste, aqueous solution after volatile oil (adding an amount of tween 80 mixing), the distillations such as adding Flos Lonicerae is made medicine activity component of the present invention.
Embodiment 27
A. extracting honeysuckle 145g, Radix Scutellariae 145g, Herba Schizonepetae 145g, Fructus Gardeniae 145g, Fructus Forsythiae 145g, Radix Scrophulariae 145g, Bombyx Batryticatus 85g, Radix Rehmanniae 215g, Rhizoma Belamcandae 45g, Radix Platycodonis 85g, Herba Menthae 85g, Periostracum Cicadae 85g, Radix Saposhnikoviae 85g, Radix Glycyrrhizae 45g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 2 times of water gagings, soak after 2 hours, adopt steam distillation 5 hours, collect volatile oil; With medicinal residues behind the 4 flavor medical material water vapour distillation such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10 times of amount 95% ethanol, ultrasonic power 5000w, 90 ℃, supersound extraction 3 times, each 20 minutes, merge extractive liquid, filters filtrate simmer down to clear paste, aqueous solution after volatile oil (adding an amount of tween 80 mixing), the distillations such as adding Flos Lonicerae is made medicine activity component of the present invention.
Embodiment 28
A. extracting honeysuckle 53g, Radix Scutellariae 53g, Herba Schizonepetae 55g, Fructus Gardeniae 55g, Fructus Forsythiae 55g, Radix Scrophulariae 55g, Bombyx Batryticatus 35g, Radix Rehmanniae 90g, Rhizoma Belamcandae 20g, Radix Platycodonis 35g, Herba Menthae 35g, Radix Saposhnikoviae 35g, Periostracum Cicadae 35g, Radix Glycyrrhizae 20g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 8 times of water gagings, soak after 2 hours, adopt steam distillation 4 hours, collect volatile oil; With medicinal residues behind the 4 flavor medical material water vapour distillation such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 15mm medical material granularity, 12 times of amount 60% ethanol, ultrasonic power 2500w, 65 ℃, supersound extraction 3 times, each 60 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds the volatile oil that vapor distillation such as above-mentioned Flos Lonicerae obtains, and makes tablet with suitable adjuvant.
Embodiment 29
A. extracting honeysuckle 89g, Radix Scutellariae 80g, Herba Schizonepetae 80g, Fructus Gardeniae 80g, Fructus Forsythiae 65g, Radix Scrophulariae 65g, Bombyx Batryticatus 55g, Radix Rehmanniae 120g, Rhizoma Belamcandae 25g, Radix Platycodonis 50g, Herba Menthae 50g, Radix Saposhnikoviae 50g, Periostracum Cicadae 50g, Radix Glycyrrhizae 30g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 18Mpa, 40 ℃ of extraction temperature, extraction time 60min, extract is standby; With medicinal residues behind the 4 flavor medical material supercritical extractions such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 8mm medical material granularity, 8 times of amount 30% ethanol, ultrasonic power 1000w, 40～70 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds CO such as above-mentioned Flos Lonicerae 2Supercritical extract mixes with suitable adjuvant, makes pill.
Embodiment 31
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; With medicinal residues behind the 4 flavor medical material water vapour distillation such as Flos Lonicerae and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid,, filter, relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, added volatile oil (adding an amount of tween 80 mixing) such as Flos Lonicerae, add adjuvants such as an amount of correctives, make capsule.
Embodiment 32
A. extracting honeysuckle 72g, Radix Scutellariae 72g, Herba Schizonepetae 72g, Fructus Gardeniae 72g, Fructus Forsythiae 72g, Radix Scrophulariae 72g, Bombyx Batryticatus 43g, Radix Rehmanniae 108g, Rhizoma Belamcandae 22g, Radix Platycodonis 43g, Herba Menthae 43g, Periostracum Cicadae 43g, Radix Saposhnikoviae 43g, Radix Glycyrrhizae 22g are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials adopt CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With 10 flavor medical materials such as the medicinal residues behind the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae, 8mm medical material granularity, 9 times of amount 40% ethanol, ultrasonic power 1200w; 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, and relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, added the CO such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness 2Supercritical extract is with the Flos Lonicerae CO of an amount of polysorbate-80 mixing 2Supercritical extract adds adjuvants such as an amount of correctives, makes granule.

Claims (10)

1. treat the cold medicine compositions for one kind, it is characterized in that adopting following method preparation:
A. 36～145 parts of extracting honeysuckles, 36～145 parts of Radix Scutellariaes, 36～145 parts of Herba Schizonepetae, 36～145 parts of Fructus Gardeniaes, 36～145 parts of Fructus Forsythiaes, 36～145 parts of Radix Scrophulariaes, 22～85 parts of Bombyx Batryticatus, 55～215 parts of Radix Rehmanniae, 11～45 parts of Rhizoma Belamcandae, 22～85 parts of Radix Platycodoniss, 22～85 parts of Herba Menthaes, 22～85 parts of Periostracum Cicadaes, 22～85 parts of Radix Saposhnikoviaes, 11～45 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 1～10 times of water gaging, soak after 0.2～3 hour, adopt steam distillation 0.5～6 hour, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 10～35Mpa, 20～90 ℃ of extraction temperature, extraction time 10～240min collect extract; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 3～15 times of water gagings or 1～95% ethanol, ultrasonic power 300～5000w, 20～90 ℃, supersound extraction 1～4 time, each 10～120 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made present composition active component.
2. treat the cold medicine compositions according to claim 1, it is characterized in that adopting following method preparation:
A. 53.54～86.70 parts of extracting honeysuckles, 53.54～86.70 parts of Radix Scutellariaes, 53.54～86.70 parts of Herba Schizonepetae, 53.54～86.70 parts of Fructus Gardeniaes, 53.54～86.70 parts of Fructus Forsythiaes, 53.54～86.70 parts of Radix Scrophulariaes, 33.57～51.55 parts of Bombyx Batryticatus, 86.27～129.81 parts of Radix Rehmanniae, 17.54～26.70 parts of Rhizoma Belamcandae, 33.57～51.55 parts of Radix Platycodoniss, 33.57～51.55 parts of Herba Menthaes, 33.57～51.55 parts of Radix Saposhnikoviaes, 33.57～51.55 parts of Periostracum Cicadaes, 17.54～26.70 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 2～8 times of water gagings, soak after 0.5～2 hour, adopt steam distillation 2～4 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 18～25Mpa, 40～60 ℃ of extraction temperature, extraction time 60～180min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2～15mm medical material granularity, 8～12 times of water gagings or 30～60% ethanol, ultrasonic power 1000～2500w, 40～70 ℃, supersound extraction 1～3 time, each 20～60 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made present composition active component.
3. as treatment cold medicine compositions as described in the claim 2, it is characterized in that adopting following method preparation:
A. 72 parts of extracting honeysuckles, 72 parts of Radix Scutellariaes, 72 parts of Herba Schizonepetae, 72 parts of Fructus Gardeniaes, 72 parts of Fructus Forsythiaes, 72 parts of Radix Scrophulariaes, 43 parts of Bombyx Batryticatus, 108 parts of Radix Rehmanniae, 22 parts of Rhizoma Belamcandae, 43 parts of Radix Platycodoniss, 43 parts of Herba Menthaes, 43 parts of Periostracum Cicadaes, 43 parts of Radix Saposhnikoviaes, 22 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues after the 4 flavor medical material supercritical extraction such as Flos Lonicerae and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, perhaps 8mm medical material granularity is measured 40% ethanol, ultrasonic power 1200w for 9 times; 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, and relative density was 1.06～1.11 when filtrate was concentrated into 70 ℃, added the extracts such as Flos Lonicerae of an amount of cosolvent mixing of above-mentioned usefulness, made present composition active component.
4. treat the cold medicine compositions according to claim 1, it is characterized in that, Fructus Gardeniae is a Fructus Gardeniae (parched); Bombyx Batryticatus is the processed with Rhizoma Zingiberis Recens Bombyx Batryticatus.
As claim 1～4 arbitrary as described in treatment cold medicine compositions, it is characterized in that, get present composition active component, make preparation with suitable adjuvant.
6. as treatment cold medicine compositions as described in the claim 5, it is characterized in that getting present composition active component, make tablet, capsule, soft capsule, oral liquid, granule, drop pill with suitable adjuvant.
7. treat cold medicine preparation of compositions method for one kind, it is characterized in that step is as follows:
A. 36～145 parts of extracting honeysuckles, 36～145 parts of Radix Scutellariaes, 36～145 parts of Herba Schizonepetae, 36～145 parts of Fructus Gardeniaes, 36～145 parts of Fructus Forsythiaes, 36～145 parts of Radix Scrophulariaes, 22～85 parts of Bombyx Batryticatus, 55～215 parts of Radix Rehmanniae, 11～45 parts of Rhizoma Belamcandae, 22～85 parts of Radix Platycodoniss, 22～85 parts of Herba Menthaes, 22～85 parts of Periostracum Cicadaes, 22～85 parts of Radix Saposhnikoviaes, 11～45 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 1～10 times of water gaging, soak after 0.2～3 hour, adopt steam distillation 0.5～6 hour, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 10～35Mpa, 20～90 ℃ of extraction temperature, extraction time 10～240min collect extract; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 3～15 times of water gagings or 1～95% ethanol, ultrasonic power 300～5000w, 20～90 ℃, supersound extraction 1～4 time, each 10～120 minutes, merge extractive liquid,, filter, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made preparation with suitable adjuvant.
8. as treatment cold medicine preparation of compositions method as described in the claim 7, it is characterized in that step is as follows:
A. 53.54～86.70 parts of extracting honeysuckles, 53.54～86.70 parts of Radix Scutellariaes, 53.54～86.70 parts of Herba Schizonepetae, 53.54～86.70 parts of Fructus Gardeniaes, 53.54～86.70 parts of Fructus Forsythiaes, 53.54～86.70 parts of Radix Scrophulariaes, 33.57～51.55 parts of Bombyx Batryticatus, 86.27～129.81 parts of Radix Rehmanniae, 17.54～26.70 parts of Rhizoma Belamcandae, 33.57～51.55 parts of Radix Platycodoniss, 33.57～51.55 parts of Herba Menthaes, 33.57～51.55 parts of Radix Saposhnikoviaes, 33.57～51.55 parts of Periostracum Cicadaes, 17.54～26.70 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 2～8 times of water gagings, soak after 0.5～2 hour, adopt steam distillation 2～4 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 18～25Mpa, 40～60 ℃ of extraction temperature, extraction time 60～180min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and all the other Radix Scutellariaes, Fructus Gardeniae etc. 10 medical materials of distinguishing the flavor of, 2～15mm medical material granularity, 8～12 times of water gagings or 30～60% ethanol, ultrasonic power 1000～2500w, 40～70 ℃, supersound extraction 1～3 time, each 20～60 minutes, merge extractive liquid, filtered, filtrate simmer down to clear paste adds volatile oil or CO that vapor distillation such as above-mentioned Flos Lonicerae obtains 2Supercritical extract is made preparation with suitable adjuvant.
9. as treatment cold medicine preparation of compositions method as described in the claim 8, it is characterized in that step is as follows:
A. 72 parts of extracting honeysuckles, 72 parts of Radix Scutellariaes, 72 parts of Herba Schizonepetae, 72 parts of Fructus Gardeniaes, 72 parts of Fructus Forsythiaes, 72 parts of Radix Scrophulariaes, 43 parts of Bombyx Batryticatus, 108 parts of Radix Rehmanniae, 22 parts of Rhizoma Belamcandae, 43 parts of Radix Platycodoniss, 43 parts of Herba Menthaes, 43 parts of Periostracum Cicadaes, 43 parts of Radix Saposhnikoviaes, 22 parts in Radix Glycyrrhizae are standby;
B. extracting honeysuckle, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials merge together, add 4 times of water gagings, soak after 1 hour, adopt steam distillation 3 hours, collect volatile oil; Or with Flos Lonicerae, Herba Schizonepetae, Radix Saposhnikoviae, Herba Menthae 4 flavor medical materials employing CO 2Supercritical extraction, extracting pressure 22Mpa, 45 ℃ of extraction temperature, extraction time 120min, extract is standby; With medicinal residues behind 4 flavor medical material water vapour distillation such as Flos Lonicerae or the supercritical extraction and 10 flavor medical materials such as all the other Radix Scutellariaes, Fructus Gardeniae, 10mm medical material granularity, 11 times of water gagings, ultrasonic power 1800w, perhaps 8mm medical material granularity is measured 40% ethanol, ultrasonic power 1200w for 9 times; 60 ℃, supersound extraction 2 times, each 30 minutes, merge extractive liquid, filtered, and relative density was 1.06～1.11 clear paste when filtrate was concentrated into 70 ℃, added the volatile oil or the CO of vapor distillations such as the Flos Lonicerae acquisition of an amount of cosolvent mixing of above-mentioned usefulness 2Supercritical extract is made preparation with suitable adjuvant.
10. as treatment cold medicine preparation of compositions method as described in the claim 9, it is characterized in that among the step b volatile oil or the CO that will obtain with the vapor distillations such as Flos Lonicerae of an amount of polysorbate-80 mixing 2Supercritical extract adds adjuvants such as an amount of correctives, adds water and adjusts total amount to 1000ml, stirs evenly, filter, and embedding, sterilization, promptly.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104225651A (en) * 2014-08-26 2014-12-24 赵兰 Air freshener for preventing influenza and preparation method thereof
CN104857203A (en) * 2015-02-03 2015-08-26 吉林省通化博祥药业股份有限公司 Method for preparing compound anti-cold softgel preparation by using ultrasonic extraction
CN105311362A (en) * 2014-07-04 2016-02-10 蚌埠丰原涂山制药有限公司 Chinese herbal medicinal composition for treating hyperlipidemia, and preparation method thereof
CN105456834A (en) * 2016-01-14 2016-04-06 江西京通美联药业有限公司 Preparation method of medicine preparation for treating common cold in children
CN112316032A (en) * 2020-11-27 2021-02-05 广州王老吉药业股份有限公司 Application of traditional Chinese medicine composition in preparation of novel coronavirus resistant medicines

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105311362A (en) * 2014-07-04 2016-02-10 蚌埠丰原涂山制药有限公司 Chinese herbal medicinal composition for treating hyperlipidemia, and preparation method thereof
CN104225651A (en) * 2014-08-26 2014-12-24 赵兰 Air freshener for preventing influenza and preparation method thereof
CN104857203A (en) * 2015-02-03 2015-08-26 吉林省通化博祥药业股份有限公司 Method for preparing compound anti-cold softgel preparation by using ultrasonic extraction
CN105456834A (en) * 2016-01-14 2016-04-06 江西京通美联药业有限公司 Preparation method of medicine preparation for treating common cold in children
CN112316032A (en) * 2020-11-27 2021-02-05 广州王老吉药业股份有限公司 Application of traditional Chinese medicine composition in preparation of novel coronavirus resistant medicines

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