CN101250559B - Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent - Google Patents
Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent Download PDFInfo
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- CN101250559B CN101250559B CN2008100707498A CN200810070749A CN101250559B CN 101250559 B CN101250559 B CN 101250559B CN 2008100707498 A CN2008100707498 A CN 2008100707498A CN 200810070749 A CN200810070749 A CN 200810070749A CN 101250559 B CN101250559 B CN 101250559B
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- dark plum
- extracting solution
- antibacterial agent
- fermentation
- bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention relates to a method for utilizing natural antibacterial agent to control alcohol fermentation infected bacteria and provides a method for utilizing the natural antibacterial agent to control the alcohol fermentation infected bacteria, which not only can control the growth of bacterial population and can reduce the amount of organic aid which is produced by bacteria, but also can promote the growth of yeast and can increase the output of alcohol. The method comprises following steps: taking dark plum, crushing the dark plum to 20-40 mesh fine powder, adding deionized water to extract 1-240 min, centrifuging, then, collecting supernatant fluid and further condensing until the content of the dark plum in extracting solution is 0.5-1g/ML, obtaining concentrated solution, obtaining dark plum extracting solution to reserve through moist heat sterilization, and adding the dark plum extracting solution 2%-15% of the fermentation broth amount according to the volume fraction in an alcohol fermentation system. Since the dark plum contains various organic acid, the method has strong inhibitory effect to gram-positive bacteria, dark plum extracts are natural plant extracts, drug residue is not available, pollution is not available, the cost is low, and the method has economic feasibility and is suitable for industrial production with large scale.
Description
Technical field
The present invention relates to a kind of method of utilizing natural antibacterial agent control bacteria pollution of alcoholic fermentation.
Background technology
The fermentative production of fuel alcohol just develops rapidly in the whole world, and a large amount of research just is being devoted to adopt cheaply biomass to come instead of corn as fermentation substrate, but seldom is concerned about in fermentation problem such as cause damage because of microbiological contamination.The typical polluted bacteria that can run in zymamsis factory has: bacillus wehmeri, Lactobacillus plantarum, no lactic acid lactobacterium casei, lactobacterium casei, short lactobacillus, Lactobacterium acidophilum, woods lactobacillus delbruckii, Leuconostoc mesenteroides, bacillus brevis, bacillus cereus (referring to Chinese patent CN 1095415A) acetic bacteria and butyric bacteria etc.Milk-acid bacteria is a contaminated bacteria main in the zymamsis, because it can survive under acid and higher temperature conditions, and its product lactic acid also suppresses the zymic growth and ferments.The method of industrial general employing pickling is prevented and treated microbiological contamination, and the yeast suspension after being about to cultivate is transferred pH to 2.0 with acid, and keeps certain hour so that milk-acid bacteria can not survive (J.Inst.Brew., 1989,95,347).But this method is infeasible in continuously fermenting.Other method has the microbiotic of employing such as penicillin (Appl.Environ.Microbiol., 1960,8,263; Appl.Microbial.Biotechnol., 2003,62,498), tsiklomitsin (Appl.Environ.Microbiol., 1960,8,263), vancomycin (J.Ind.Microbiol.Biotechnol., 1997,18,284) etc.But the price of microbiotic costliness makes the fermentation industry profit lower, and the appearance of residues of antibiotics problem and drug tolerant bacteria makes various countries all formulate corresponding law to reduce the use microbiotic.
In recent years, polyether ionophore antibiotic (referring to Chinese patent CN 1095415A), sulphite and the hydrogen peroxide (Appl.Environ.Microbiol. of employing also arranged, 1997,63,1.), Trichlorocarbanilide (Biores.Technol., 1998,63,17.) wait the pollution of controlling milk-acid bacteria.U.S. Pat 20070036882A1 has reported from hops and has separated 6 kinds of hops acid obtaining in 2～20ppm (10
-6G/mL) growth that can lactic acid bacteria inhibiting, and then improved the output of alcohol.Adopt natural antibacterial agent control bacteria pollution of alcoholic fermentation can use method such as microbiotic to compare favourably,, and do not pollute, meet " green " demand because it not only plays and suppress assorted bacterium, promote the effect of fermentation with tradition.
Summary of the invention
Purpose of the present invention aims to provide a kind of growth of not only may command bacterial population, reduces by bacteriogenic organic acid amount, and promotes saccharomycetic growth, improves the method for utilizing natural antibacterial agent control bacteria pollution of alcoholic fermentation of alcohol output.
The present invention includes following steps:
1) gets dark plum and be crushed to 20～40 order fine powders;
2) add deionized water and extract 1～240min;
3) the centrifugal back content collecting supernatant liquor and further be concentrated into dark plum in the extracting solution is 0.5～1g/mL, concentrated solution;
4) it is standby concentrated solution to be got the dark plum extracting solution through moist heat sterilization;
5) in the fermentation system of alcohol, by volume 2%～15% of the fermentation liquid measure of per-cent adds the dark plum extracting solution.
Extraction described in the step 2 can be adopted modes such as refluxing extraction, microwave extraction, supersound extraction or supercritical extraction.
The present invention is applicable to as Angel alcohol active dried yeast, rice wine yeast, K-yeast, 1300 distillery yeasts, Sb724 distillery yeast, and the motion Zymomonas mobilis distillery yeasts such as (bacteriums) that is applied to ferment.
The present invention is applicable to starchy materials such as rice, corn, jowar, cassava, and beet syrup, sugarcane syrup, the beet waste molasses of dilution, the cane molasses of dilution, grape wine, grape wine by product, fermentation substrates such as hard cider or hard cider by product.
The present invention promotes the output of zymic growth and fermentation and then raising alcohol by add the growth that the dark plum extracting solution is controlled milk-acid bacteria in fermented liquid.Wherein,, gram-positive microorganism there is had strong inhibitory effects, and can promotes the zymic growth, and then make alcohol output improve 1.2～5.4% because dark plum contains various organic acids.And Fructus Mume extract is extracted form natural plant, and the drug residue free problem does not pollute, and wide material sources are cheap.Therefore, this method has economic feasibility, is adapted to large-scale industrial production.
Embodiment
The invention will be further described below by specific embodiment.
Embodiment 1
Get dark plum earlier and be crushed to 20 order fine powders, take by weighing 30g, the deionized water that adds 240mL carries out refluxing extraction 1.5h, and centrifugal back is collected supernatant liquor and further is concentrated into 60mL, and moist heat sterilization gets the dark plum extracting solution that concentration is 0.5g/mL (being equivalent to contain in every milliliter of extracting solution 0.5 gram dark plum) again.Cultivate rice wine yeast and lactobacillus fermentum ATCC 14931 (lactobacillus fermentum ATCC14931 can be in 30 ℃ of following 36h raised growth, and can under＞10% (v/v) alcohol, survive (referring to document: Appl.Environ.Microbiol., 2000,66,4187.) to logarithmic phase.Will corn steep liquor after the liquefaction, saccharification fermentable sugar reaches～60mg/ml, it is that the bottle that shakes of 250ml contains the 100ml fermented liquid that packing makes each volume, inserts yeast suspension and milk-acid bacteria bacteria suspension again, make yeast and milk-acid bacteria reach respectively～10
8CFU/mL (colony-forming unit in every milliliter) and～10
8CFU/mL, and then add 4ml dark plum extracting solution.Every the 12h sampling, with the amount of alcohol and lactic acid in the high-performance liquid chromatogram determination sample.Alcohol output when recording fermentation ends is 9.40mg/ml, and lactic acid production is 0.17mg/ml.Corresponding enumeration result is: the yeast cell number is by initial 1.6 * 10
8CFU/mL is 5.6 * 10 behind 12h
8CFU/mL; Be 0.6 * 10 when the milk-acid bacteria number is initial
8CFU/mL is 2.2 * 10 during fermentation ends
9CFU/mL.
Embodiment 2
Embodiment such as embodiment 1, different is the dark plum extracting solution that adds 8mL behind yeast that inserts same amount and milk-acid bacteria again.Every the 12h sampling, with the amount of alcohol and lactic acid in the high-performance liquid chromatogram determination sample.Alcohol output when recording fermentation ends is 9.60mg/ml, and lactic acid production is 0.10mg/ml.Corresponding enumeration result is: the yeast cell number is by initial 1.6 * 10
8CFU/mL reaches 3.9 * 10 behind 12h
8CFU/mL; Be 0.6 * 10 when the milk-acid bacteria number is initial
8CFU/mL is 1.6 * 10 during fermentation ends
8CFU/mL.
Embodiment 3
Embodiment such as embodiment 1, the dark plum extracting solution changes 15mL into.Every the 12h sampling, with the amount of alcohol and lactic acid in the high-performance liquid chromatogram determination sample.Alcohol output when recording fermentation ends is 9.80mg/ml, and lactic acid production is reduced to 0mg/mL.Corresponding enumeration result is: the yeast cell number is by initial 1.6 * 10
8CFU/mL reaches 3.2 * 10 behind 12h
8CFU/mL; Be 0.6 * 10 when the milk-acid bacteria number is initial
8CFU/mL is 1.4 * 10 during fermentation ends
6CFU/mL.
Embodiment 4
Embodiment such as embodiment 1 get dark plum and are crushed to 40 order fine powders, take by weighing 60g, add the deionized water of 240mL, extract 60s at microwave power during for 700W, the centrifugal 60mL that is concentrated into, and moist heat sterilization gets the dark plum extracting solution that concentration is 1g/mL again.The dark plum extracting solution that after inserting, adds 5mL again with the yeast of embodiment 1 amount and milk-acid bacteria.Every the 12h sampling, with the amount of alcohol and lactic acid in the high-performance liquid chromatogram determination sample.Alcohol output when recording fermentation ends is 9.55mg/ml, and lactic acid production is 0.12mg/ml.Corresponding enumeration result is: the yeast cell number is by initial 1.6 * 10
8CFU/mL reaches 3.5 * 10 behind 12h
8CFU/mL; Be 0.6 * 10 when the milk-acid bacteria number is initial
8CFU/mL is 4.0 * 10 during fermentation ends
8CFU/mL.
Comparative Examples 1
Embodiment such as embodiment 1, different is yeast and the milk-acid bacteria of only inserting same amount, does not add the dark plum extracting solution.Every the 12h sampling, with the amount of alcohol and lactic acid in the high-performance liquid chromatogram determination sample.Alcohol output when recording fermentation ends is 9.30mg/ml, and lactic acid production is 0.21mg/mL.The zymic number of cells is by being initiated with 1.6 * 10
8CFU/mL reaches 3.0 * 10 behind 12h
8CFU/mL; And the number of milk-acid bacteria is 0.6 * 10 when initial
8CFU/mL is 3.9 * 10 during fermentation ends
9CFU/mL.The comparison of Comparative Examples and embodiment can see Table 1 and table 2.
Table 1 dark plum extracting solution is to the influence of lactobacter growth in the zymamsis
Table 2 dark plum extracting solution is to the influence of yeast growth in the zymamsis
Claims (2)
1. method of utilizing natural antibacterial agent control bacteria pollution of alcoholic fermentation is characterized in that may further comprise the steps:
1) gets dark plum and be crushed to 20～40 order fine powders;
2) add deionized water and extract 1～240min;
3) centrifugal back is collected supernatant liquor and is further concentrated, and to get concentration be that the dark plum extracting solution of 0.5～1g/mL is standby to moist heat sterilization again;
4) in the fermentation system of saccharomycetic alcohol, by volume 2%～15% of the fermentation liquid measure of per-cent adds the dark plum extracting solution.
2. a kind of method of utilizing natural antibacterial agent control bacteria pollution of alcoholic fermentation as claimed in claim 1 is characterized in that being extracted as refluxing extraction, microwave extraction, supersound extraction or supercritical extraction described in the step 2.
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| CN2008100707498A CN101250559B (en) | 2008-03-14 | 2008-03-14 | Method for preventing bacteria pollution of alcoholic fermentation by using natural antibacterial agent |
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| CN101250559B true CN101250559B (en) | 2010-12-29 |
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| CN112408408A (en) * | 2020-12-02 | 2021-02-26 | 吉林大学 | Application of composition containing active mica in antibacterial activity |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095415A (en) * | 1992-01-27 | 1994-11-23 | 国家造酒集团联盟 | Utilize the growth of polyether ionophore antibiotic control zymamsis bacterium |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095415A (en) * | 1992-01-27 | 1994-11-23 | 国家造酒集团联盟 | Utilize the growth of polyether ionophore antibiotic control zymamsis bacterium |
Non-Patent Citations (2)
| Title |
|---|
| Narendranath,N.V.等.Effects of lactobacilli on yeast-catalyzed ethanol fermentations.《Applied and Environmental Microbiology》.1997,第63卷(第11期),4158-4163. * |
| Narendranath,N.V.等.Relationship between pH and medium dissolved solids in terms of growth and metabolism of Lactobacilli and Saccharomyces cerevisiae during ethanol production.《Applied and Environmental Microbiology》.2005,第71卷(第5期),2239-2243. * |
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