CN112408408A - Application of composition containing active mica in antibacterial activity - Google Patents
Application of composition containing active mica in antibacterial activity Download PDFInfo
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- CN112408408A CN112408408A CN202011403502.0A CN202011403502A CN112408408A CN 112408408 A CN112408408 A CN 112408408A CN 202011403502 A CN202011403502 A CN 202011403502A CN 112408408 A CN112408408 A CN 112408408A
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- mica
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B33/00—Silicon; Compounds thereof
- C01B33/20—Silicates
- C01B33/36—Silicates having base-exchange properties but not having molecular sieve properties
- C01B33/38—Layered base-exchange silicates, e.g. clays, micas or alkali metal silicates of kenyaite or magadiite type
- C01B33/42—Micas ; Interstratified clay-mica products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract
The invention provides an application of a composition containing active mica in bacteriostatic activity, belonging to the technical field of biology. The invention uses the compositions containing the active mica with different concentrations to act on the liquid bacterial liquid, cultures and samples, and measures the absorbance of the liquid bacterial liquid at 600nm to judge the bacteriostatic action of the composition containing the active mica. The results show that the bacterial concentration decreases with increasing amounts of the composition containing the active mica, over a range of amounts. Therefore, the composition can obviously inhibit the activity of escherichia coli and staphylococcus aureus, and has the potential of becoming a novel antibacterial drug.
Description
Technical Field
The invention relates to the technical field of biology, in particular to application of a composition containing active mica in antibacterial activity.
Background
Mica is a rock-making mineral, presents a hexagonal flaky crystal form, is one of the main rock-making minerals, and is also an important traditional Chinese medicine substance. The traditional Chinese medicine mica is silicate mineral muscovite, and is prepared by collecting, cleaning soil, and removing miscellaneous stones. Mention is made of "Ben Cao Jing Shu" (herbal Regulation): mica and stone are considered as good things as fire when they fall down and water when they fall down. Therefore, it can nourish five zang organs, benefit essence of the seed and improve vision. The Shennong Ben Cao Jing also records the efficacy of mica, and the record shows that: governing dead skin and wind-stroke with cold and heat, for example, on vehicles and ships, remove pathogenic qi, calm five internal organs, benefit sperm and improve eyesight. The prior patent CN 110628826A discloses a composition containing active mica and a biological fermentation preparation method thereof, the composition containing active mica obtained in the patent document is rich in effective active components, has the capability of degrading organic pollutants and has the health-care effect; it can also be used for preparing sunscreen, disinfectant, air purifier, etc. Therefore, the mica has stronger functions as a mineral or a traditional Chinese medicine component.
At present, the wide use of traditional antibiotics causes the drug resistance of germs to be continuously enhanced, more and more drug-resistant strains exist, and an advantageous substitute which has a wide antibacterial spectrum and is not easy to generate drug resistance is urgently needed. Based on the significant pharmaceutical value of mica in the prior art, further research into compositions containing active mica is needed.
Disclosure of Invention
The invention aims to provide an application of a composition containing active mica in bacteriostatic activity. Research shows that the active mica composition provided by the invention has obvious inhibition effect on escherichia coli and staphylococcus aureus in a certain dosage range, and the active mica composition has certain potential in preparation of antibacterial drugs.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of a composition containing active mica in preparing bacteriostatic and/or antibacterial products.
In the present invention, the bacterium is a bacterium.
In the present invention, the bacteria are Escherichia coli and Staphylococcus aureus.
In the present invention, the product is a medicament, a feed additive or a tooth desensitizer.
The invention is characterized in that the weight percentage of the active mica composition in the product is 4.5-100%.
In the invention, the active mica composition is fermentation liquor obtained by fermenting silicate minerals and plum fruits in a solvent.
In the present invention, the solvent is at least one selected from water, alcohol solvents, and ether solvents.
In the invention, the weight ratio of the silicate mineral, the plum fruit and the solvent is 2-6: 0.5-2: 3 to 8.
In the invention, the total fermentation time is 130-150 days.
In the present invention, the silicate mineral has a particle size of 0.5 to 0.8 cm.
The invention has the beneficial effects that: the invention provides an application of a composition containing active mica in bacteriostatic activity. The bacteriostatic action of the composition containing active mica is judged by applying 5 concentrations of 0, 0.25, 0.5, 1, 2mL of the composition containing active mica to a liquid bacterial solution, wherein the bacterial species are preferably Escherichia coli (E.coli ATCC 25922) and Staphylococcus aureus (S.aureus ATCC 25923), sufficiently culturing and sampling, and measuring the absorbance at 600 nm. The results show that the absorbance of the bacterial liquid at 600nm is obviously reduced along with the increase of the dosage of the composition containing the active mica. The results show that the composition containing the active mica has remarkable bacteriostatic activity.
Drawings
FIG. 1 is a graph showing the absorbance at 600nm of E.coli bacteria in different concentrations of active mica-containing compositions;
FIG. 2 is a graph of the absorbance at 600nm of Staphylococcus aureus in various concentrations of compositions containing active mica.
Detailed Description
The invention provides application of a composition containing active mica in preparing bacteriostatic and/or antibacterial products.
In the present invention, the bacterium is a bacterium. In the invention, the bacteriostasis and/or antibiosis test method specifically comprises the following steps: 1) preparing a bacteria culture medium and sterilizing; 2) inoculating strains into the culture medium in the step 1) for culture; 3) diluting the fermentation broth of the composition containing the active mica with different amounts to the same volume by using the culture medium in the step 1) to prepare the fermentation diluents of the composition containing the active mica with different concentrations; 4) adding the bacterial liquid obtained in the step 2) into the solution obtained in the step 3) for culture; 5) at regular intervals, the step 4) was sampled and the absorbance at 600nm was measured. The sampling in the steps 2), 3), 4) and 5) needs to be carried out in a sterile manner in a clean bench. In the present invention, the bacterial culture medium may be any liquid bacterial culture medium. In a specific embodiment of the present invention, the bacteria culture medium is preferably MHB (Mueller-Hinton Broth) culture medium, and is preferably prepared by: 21.0g of MHB medium solid was weighed out and dissolved in 1000mL of distilled water. The conditions for the bacterial culture preferably include: the temperature is 37 ℃, the rotating speed is 180rpm, and the culture time is preferably 12-20 h, more preferably 14-18 h, and most preferably 16 h. The absorbance is preferably measured by an ultraviolet-visible spectrophotometer. In the present invention, the source of the raw material used in the test method is not particularly limited, and a conventional commercially available product known to those skilled in the art may be used.
In the present invention, the bacteriostatic or antibacterial activity of the active mica composition was measured with escherichia coli and staphylococcus aureus as representatives of the bacteria. In the present invention, the species are preferably escherichia coli (e.coli ATCC 25922) and staphylococcus aureus (s.aureus ATCC 25923). In the invention, the strain bacterial liquid is frozen and stored automatically in key laboratories of the department of molecular and enzymology engineering education of Jilin university, and the freezing and storing method is preferably as follows: when the bacteria are amplified in the culture medium until the OD600 value is 0.5-0.6, 500 mu L of bacterial liquid and 500 mu L of sterilized 50% glycerol aqueous solution are uniformly mixed and frozen at-80 ℃.
In the present invention, the product is a medicament, feed additive or tooth desensitizer. In the present invention, the active mica composition is added to the drug, feed additive or tooth desensitizer. In the present invention, the specific preparation method of the drug, feed additive or tooth desensitizer is not particularly limited, and the preparation process known in the art can be employed.
In the invention, the weight percentage of the active mica composition in the product is 4-100%, preferably 9-60%, more preferably 30-40%, so that the prepared product has certain bacteriostatic and/or antibacterial activity.
In the present invention, the active mica composition is a fermentation broth obtained by fermenting silicate minerals and plum fruits in a solvent. In the present invention, a preferable fermentation method can be found in the method for preparing the composition containing active mica disclosed in the patent publication No. CN 110628826A entitled composition containing active mica and its biological fermentation preparation method. In the present invention, the silicate mineral is selected from at least one of granite, perlite, mullite, metamorphic rock, basalt; the mume fructus is at least one selected from mume fructus, and prune. In the present invention, the sources of the silicate minerals and the ume fruits are not particularly limited, and conventional commercially available products known to those skilled in the art may be used.
In the present invention, the solvent is at least one selected from water, alcohol solvents, and ether solvents. In the present invention, the alcohol solvent is at least one selected from methanol, ethanol, n-propanol, n-butanol, isoamyl alcohol, ethylhexanol, ethylene glycol, and propylene glycol; the ether solvent is at least one selected from methyl ether, ethyl ether, propyl ether, isopropyl ether, butyl ether, amyl ether, isoamyl ether, hexyl ether, ethyl butyl ether, methyl tert-butyl ether, ethyl vinyl ether and butyl vinyl ether. In the present invention, the source of the solvent is not particularly limited, and a conventional commercially available product well known to those skilled in the art may be used.
In the invention, the weight ratio of the silicate mineral, the plum fruit and the solvent is 2-6: 0.5-2: 3-8, and preferably 3-5: 0.8-1.5: 4 to 7.
In the invention, the total fermentation time is 130-150 days, and more preferably 135-145 days.
In the present invention, the silicate mineral preferably has a particle size of 0.5 to 0.8 cm.
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a composition containing active mica comprises the following steps: the preparation raw materials comprise, by weight, 3 parts of silicate minerals, 1 part of plum fruits and 4 parts of solvents. Crushing silicate mineral powder to 0.5-0.8 cm and cleaning with a vibrator; removing cores from mume fructus; adding silicate mineral, mume fructus and solvent into fermenter, fermenting at 50 deg.C for 135 days, and filtering to obtain composition stock solution containing active mica.
Example 2
A preparation method of a composition containing active mica comprises the following steps: the preparation raw materials comprise 5 parts of silicate minerals, 2 parts of plum fruits and 7 parts of solvents in parts by weight. Crushing silicate mineral powder to 0.5-0.8 cm and cleaning with a vibrator; removing cores from mume fructus; adding silicate mineral, mume fructus and solvent into fermenter, fermenting at 65 deg.C for 130 days, and filtering to obtain composition stock solution containing active mica.
Example 3
A preparation method of a composition containing active mica comprises the following steps: the preparation raw materials comprise 2 parts of silicate minerals, 0.5 part of plum fruits and 3 parts of solvents in parts by weight. Crushing silicate mineral powder to 0.5-0.8 cm and cleaning with a vibrator; removing cores from mume fructus; adding silicate mineral, mume fructus and solvent into fermenter, fermenting at 45 deg.C for 145 days, and filtering to obtain composition stock solution containing active mica.
Example 4
Inhibition of E.coli by stock solutions of compositions containing activated mica
21.0g of MHB medium solid was weighed out and dissolved in 1000mL of distilled water. The prepared MHB medium was sterilized by autoclaving at 121.3 deg.C for 20 min. mu.L of E.coli strain stored in a-80 ℃ refrigerator was cultured in 2mL of MHB medium at 37 ℃ and 180rpm for 16 hours. 0, 0.25, 0.5, 1, 2mL of the stock solution of example 1 containing the active mica composition was taken in a test tube and diluted to 6mL with MHB medium. To each of the solutions containing the active mica composition at different concentrations, 10. mu.L of the cultured bacterial suspension was added and cultured at 37 ℃ and 180 rpm. And taking 500 mu L of samples every 2h, taking the solution containing the active mica composition with each concentration and not containing the bacteria liquid as blank control, carrying out zero setting, and measuring the absorbance of the bacteria liquid at 600nm under corresponding concentration and at different time by using an ultraviolet-visible spectrophotometer.
As shown in figure 1, the antibacterial effect on Escherichia coli is gradually increased with the increase of the amount of stock solution containing active mica composition, and the antibacterial rate can reach more than 90%.
Example 5
Inhibitory effect of composition stock solution containing active mica on staphylococcus aureus
21.0g of MHB medium solid was weighed out and dissolved in 1000mL of distilled water. The prepared MHB medium was sterilized by autoclaving at 121.3 deg.C for 20 min. Staphylococcus aureus strain (1. mu.L) stored in a refrigerator at-80 ℃ was cultured in 2mL of MHB medium at 37 ℃ and 180rpm for 16 hours. 0, 0.25, 0.5, 1, 2mL of the stock solution of example 1 containing the active mica composition was taken in a test tube and diluted to 6mL with MHB medium. To each of the solutions containing the active mica composition at different concentrations, 10. mu.L of the cultured bacterial suspension was added and cultured at 37 ℃ and 180 rpm. Taking 500 mu L of samples every 2h, taking organic germanium solution with each concentration without bacterial liquid as blank control, carrying out zero setting, and measuring the absorbance of the bacterial liquid at 600nm under corresponding concentration and different time by using an ultraviolet-visible spectrophotometer.
As shown in figure 2, the antibacterial effect on Staphylococcus aureus is gradually increased with the increase of the amount of the stock solution containing the active mica composition, and the antibacterial rate can reach more than 90%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. Application of composition containing active mica in preparing bacteriostatic and/or antibacterial products.
2. The use according to claim 1, wherein the bacteria are bacteria.
3. Use according to claim 2, wherein the bacteria are Escherichia coli and Staphylococcus aureus.
4. Use according to claim 1, wherein the product is a medicament, a feed additive or a tooth desensitizer.
5. The use according to any one of claims 1 to 4, wherein the active mica composition is present in the product in an amount of from 4.5% to 100% by weight.
6. The use according to claim 1, wherein the active mica composition is a fermentation broth obtained by fermenting silicate minerals and plum fruits in a solvent.
7. The use according to claim 1, wherein the solvent is at least one selected from the group consisting of water, alcohol solvents, and ether solvents.
8. The use according to claim 6, wherein the weight ratio of the silicate mineral, the plum fruit and the solvent is 2-6: 0.5-2: 3 to 8.
9. The use according to claim 6, wherein the fermentation time is 130 to 150 days in total.
10. The use according to claim 6, wherein the silicate-based mineral has a particle size of 0.5 to 0.8 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011403502.0A CN112408408A (en) | 2020-12-02 | 2020-12-02 | Application of composition containing active mica in antibacterial activity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011403502.0A CN112408408A (en) | 2020-12-02 | 2020-12-02 | Application of composition containing active mica in antibacterial activity |
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| CN112408408A true CN112408408A (en) | 2021-02-26 |
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| CN202011403502.0A Pending CN112408408A (en) | 2020-12-02 | 2020-12-02 | Application of composition containing active mica in antibacterial activity |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113288912A (en) * | 2021-06-24 | 2021-08-24 | 吉林大学 | Application of mica fermentation liquor in inhibiting helicobacter pylori |
| CN114191449A (en) * | 2021-12-14 | 2022-03-18 | 吉林大学 | Antibacterial gel and preparation method and application thereof |
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2020
- 2020-12-02 CN CN202011403502.0A patent/CN112408408A/en active Pending
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| KR101803069B1 (en) * | 2017-03-20 | 2017-11-29 | 한재갑 | Composition for improving livestock immunity comprising probiotics as effective component |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113288912A (en) * | 2021-06-24 | 2021-08-24 | 吉林大学 | Application of mica fermentation liquor in inhibiting helicobacter pylori |
| CN114191449A (en) * | 2021-12-14 | 2022-03-18 | 吉林大学 | Antibacterial gel and preparation method and application thereof |
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